Cucsat.G4651.T9 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G4651.T9
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	FSH1 domain-containing protein
Location	ctg1227: 1270799 .. 1277168 (+)
RNA-Seq Expression	Cucsat.G4651.T9
Synteny	Cucsat.G4651.T9

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ACAAGAACTTGAAATATGGCAAGCGAGAGCAAAACTGAAAGGAAACCCAAGACTTTGTGCCTCCATGGCTTTCGGACTAGTGGAGCAATCTTGAGGAAGCAGGTCCAAAGATGGCCAACTTCTGTTCTTCATCAATTCCATCTCCATTTTATCGACGACTCAATCCCTTCCAAAGGAAAATCAGACTTCGAAGGAATTTACGATCCTCCCTATTTCGAGTGGTTTGGAACCAGCGAGGTCCATATTAATTGTTTTTCCTTCCTTTTTTTATTTTCAAGTCTCTCTATATTTCAAATACTGATTCATTCAATTTCTCAGGACCCGACCAATTATGAAAATTTGGAATCGAGCATTGAATTCATCGAGAGTTACATGCTTGAGCACGGACCATTCGACGGACTTCTCGGTTTTTCACAGGTAACTAAGCGTTGATTCTCTTGAATCTGATTTGGATTGAGTTAATTAATAATTTCATAATTTGAAGCTATTTTTAGGGGGCAATGTTATCGGCAGCACTGGCGTTGCTTCAAGCGAGGGTAATTAAACACAGATGGCTGGTTGTTCTTTGTTTTAATCTTTGTTTTCAATTGTCGAAATTTGAATTTTAATCGGCTTGCAGGGAGTTGCACTGACGAAGGTCCCGAAGATCAAATTCGTAATAGTAATTAGCAGTTCGAAACTGCAATCATCATCCTTGGCCGCTCGAATAGTTTATTCTACGTCTATTGCATGCCCATCCCTCCATTTTTTAAGTAACTTTCCGACATCTTCATGTTTCTCATTTTATATTTGTTTTCTTCAAAGTTCGAATCCAACCTTAAAAGAAAATTATTTAAAATATACTCTAAAAACTATTATGAATTGTAACGTTCTAAACAAAGTTATAGAGTTAGAAGATATTTTTAAAAGTTTAGGGTTTAGAGTTTACAAAAATTAATATTTACTAACTTATAACAAATCTAAAATTTTGCTATAATTTACAAATATTTTAATTTATTTTACTATTTTAAAAAATGATCCTTACAATTAGATTGAAGTGATTTTGAGATGACAACCTATACCCTCACGATCAAAACCTCCGACTTTTCTTTTTCAAAATTAAAAAAAAAAAAGAAAGAAAGACGACCTCTATCTTGTTGAGAAAGTAGAGTAAGGTAGAAAATTAATTAATTATGGTTATAATATACCACCCAATATGCTTAAATATGCAAACACTTTTTTCTTTTAATATAAAGAAAAAATACAAACAAACTATTTACTCGAAAAAAAATTATTTCATTAATTGTTTTGAAAGGTAAATATGTTAGTCTTTTTTTACAACATTTTTCCACATTGCAACGTGAGAGTTAGTTCAATATTTGAGATCGAGTCAATAAAAGTCTCGATCCGTTTGAATTGAATTAAAAAAAAAATGTTTTTAAAAATTCTTTTTTGTTAAACTCTATTTTTAAAAAACTGTTTAAAATATAGTTAAAAATTTATTTTAAATGACTTCCAAACACTATAATTCTTTTTCTAAAAACAATTTGTTTTATAAATTAAATATTTGAAATTGTAATTCAAATCAACCACTTTATTGAAAATCAAATCAATTACTGTTGATCCATTCATTTTTTAATTTTACACATTTGATTGCATTTCACATTATTAAAATTGATTTTTAAACTAGATAATTTTCAAGTAATTTATAATTAAATGACAAAACAATTTAAACCATTTTAAAATAACAGTGGTTTGCATATGTAGGTGAGGAAGACTTCTGGATGCCCAGTGGATTAAAGCTTCTACAATCATTTGTTGAACCATTAGTAATTCATCATTCTAAAGGCCACATAGTACCAAGACTTGGTAATATTTAATTTATTCACCTATCTATCTATCTACATACGTGTGGGATTTTCTTGTGGTAATTTCTATATTTATATAATTTTATTTTGCAGATGAAAAAAGTTTGAAGATTGTGAACGGATTTATCCAAAAGGTTTCAAAGTTGTGACCACGCATGAGTTTAAATAATTGAATCGAATTTACTTCTTAAATAAGTTCTCGAACTTTTATCAAAAGTATATCTTAATTAAGTACGAAGATTTAAATCGTAGAATCTTAACTTTGTTGGAATTTTATTATTACATCAACACAATACAAATATTAAAATGATAAAGTCTGAAGTATTTTTACTATTTGATAAATTTCACTACTTAATTTGATATACTAAAAGACTAATATCATTCCTCAATTTGTTAAATAAGTATAAATGTTTATTTAAAAAAAAAAAAAGAGTCCTATAAATCCGATTAATCCAAAAATAGTAATAATAATGATAAAAGCAACAAAGAATTTTCTCCAGAGATTAAATGTTTTCTTTAAATACCTAAAAATTCAGTTAACCTTATGCATTTAATTAACTTAAATAACCTTTATTACAATTTTCTAAAGAAATAAATACAAATCCCCGTAGCAACTTCATTGCTATGAAATTAACATTACTTATTAAAATCTTAAATTAATAATTTTGTATCCATCTAGAATTTTTTTTTTAATTATTATTTGAATTGAATAAGACCATTTATTAGTTATTCATAAATATTTTTGACTCCATTCCATTTACAACTTAACTCAAAATGACTTAAATTCCTTTCAAATTATACCTAATTAAGTAAGAAAATTATGTAATACATAATTCTACCTATTTAGATAAATAAAAATATAGAAATAAATGATATTGAAAAACACTGCTCATACTAATAAAAGTATCCAATTGACTCTAATGTTTGTCAGAACACTAAATTAATCTAATTCCCTAAGTGTTATAAGTATGTATTAATAATTTCGAAAAAAAAAACGTGAATCAAATTGATTTAGTCAAGAGATCCAGAGTTTGTAAAAGGTAGTTGTTAGGAATAGAACAAATATATACATATTTACAAAATATAGAAAAAGAAAAAAACGTACATAAGATTGAATGAATTTTGAATATATTTTGTAAATAGTTTTAATATTTTGTAACACGTATATTAAAACTATCTAGCTAATCTCTTTATTATTGTTGCTTTTAGTTTTATTAAATATGAAATCGATTTAAAATTAGTTTATAAGTTTTTAAAATGTTACAATTTATGTTAAGATTTTAACTTTGTTTCAATTTAGTTTCTAGAATTCAAGAAATACACTTATGACCTAAACATTTTTTTTACAAATTACTCACTTTCAATCTTTGGTATTAATTTATGTTATGCTTAAATCTTGTCTACCTTGAGTTGTGTCACAAGTGTCGAATGGTCTCGTTATTTAGCCATCTTTCCTCAGGTTGAATCTCGATATTGTTCGAACCAACTTGCAAGAAATCGTTGTATCCATATATTGCAAAACTTACTACGATTTGATGCTTAAGGTTAATATTAGAAGTAACTATTTCAACCGAGTATAAAAAAAAAAATCTAATATCATTTTTGGAGATTCTAAATTGAAATTAAAACTTTTTAAAAAAAAGTAAGTAGAAATGAATATTTATTTACTAGTTTTTATAATTGACCCCCTCACATTATCGGTGCTCCCTCGCCCCTTAATAAATCCACCTTTGGTTGATTGGGTTTTGTTTTTGCTATTTTTGTAAATAACAAAGTACGAATGCTATAAATTTAATTATCATAACTTGTTTTACTATTTTTGTAAGTGTTCCAAAATTTAAAATATAGTAATGATAAAAATATGTGATAGACGGTAGGGTAGTGTTGGTTAGAGAATACGAGCAATAAGGTTTATTACAAATAAATTTGGTTGTATTTAACATTTTTTTTTAAAAAAATTGTTGCTATATATATAATACCAATCCAATATCTAATCTTAAAACGACAATTCGTTAACTACTCTTTATAATTGACAACCCCAACCATGGGTCGAAATGTTGGCAACGTATCTCTTACCTCGTCTAAAAATTATTATAATTCAACGATGATAGCCTCAAACTGTGAAACATACTAATCAAATTATTGCCTCCTACACTACTTAACATAAACGTTTAAAGTATGAGAGATGGAACCACAAATTTTCAAGCCATCAACGATTTTCAACAGGTGGGGTCATCTTTCCTCTTACAATTCTTTCAAAAATATTCCCAAATTTCAAAACTACAACTAATCTATGAGACCTATTTAAAATGAGCAACTCCATAAACATAAAGAATGAGAACTTGAAGAATGGCAAGTGAGAGAAAAATTGAAAGGAAACCCAAAATTTTGTGCCTCCATGGCTTCCGAACTAGTGGAGCAATATTGAGGAAGCAGGTCCAAAGATGGCCAACTTCCATTCTTCATCAATTCCATCTCCATTTTATCGACGGCCCATTCCCTTCCAAAGGAAGATCAGACGTCGAAGGAATTTACGATCCTCCCTATTTCGAGTGGTTTGGAACCAGCGAGGTCCATATTAATTGCTTTTTCTTCTTCTTCTTCTTCTTTTTCAATTCTCTCTACATTTCAAATACTGATTTGTTCCATTTCTCAGGACCCGACTAGTTGTGAAAATTTGGAATCGAGCCTCGAATTCATCGAGAGTTACATGGCTGAGCACGGACCATTCGACGGACTTCTCGGTTTTTCACAGGTAATCAAGGGTTGATTCTCTTAAATCCGATTTGGATGGATTTAATTAATAATTTCATAATTTGAAGCTATTTTTAGGGGGCGGTGTTATCGGCAGCACTGGCGTTGCTTCAGGCGAGGGTAATTAAACACCGATGGCCAGTTGCTCTTCGTTTTAATCTCTGTTTTCATTTTTCGAAAATTGAATTTTAATCGGCTTGCAGGGAGTTGCACTGACGAAGGTCCCGAAGATCAAATTCGTAATAGTAATTAGCGGTTCGAAACTGCAATCATCATCCTTGGCCGCTCGAATAGCTTATTCTACATCTATTGCATGTCCATCCCTCCATTTTCTAAGTAACTTTCCGGCGTCTTCAAGTTTCTCATTTTATATTTTTTCTGTTTAAAGTTCGAATCATCTTAAAGAAAATTATTTAAAACACACTCTAAAAACTATTATAGTTATTGAATTAGAAGATATCTTGGATAGAGTTCAAAGTTTACTAATACATATGATAAGACTATTATTGATATAATTAAAATTTTGTAATGTTTTGTAAATATTTTAATTTATTTTATTATTCTTAAAAGCGACACTTACATATATATATATATTGATCTATACTTTTACTATCAAAATCTCTAACTTTTTTTTTCAATTTTTTTTTTTAAAACAACCTCTATCTTGTTGAGAAAGTAGAGAGTAGCGATGTATTATGATAGAAAATTAATTAATTATTGTTATAATATACCACCCAATATGTTTAAATATGCTAAGGGGTTTTTTTAATATAAAGAAAAAAGAAACAAACAAATTATTTACTAAAAAGGTTATTTCATTATTTTTTGGAATTTTTTTTAAATATTAAATTTTATAAAATATTTACTAAGAAGAAATGTTTTTCAACAAATTCATTTTTATTAAAATGTTTAAAATATAATTTAAAATATATTTTGAATTACACTTGAAGATATAATTTAAATGCACCATACAAAACAATAAACCATGAAAATCAAATCAATAATTTTTATTCAAAATCAAACCATCAAATGCCGTGATGATTGATTGAAAATCAAATCAATTACTCTTAGATCAATTCAATTTTTAGTTTTACTCCTTTCACATTATTAAAATTGATTTTTAAACTACTGAGATATTTTTCAAGTAATTTATAATTAAAATGACTACACAATTTAAACCACTTTAAAATATGGTAAATTTCATTTTATGAACAATAATTAAGTATATAGCAAAACTATAGACTTCTGTCATTGATAGAATTTGATAAATTTTATTATATTTGTAAATTTTTTAAAATGTTGTTATGTACTTAATTATTTTAAATCTAATTGTTAAATTTACAACTATCTCTTCAAAATACTGAGAATTGCATACGTAGGTGAGGAAGACTTCTTGATGCCCAGTGGGTTAAAGCTTCTAGAATCATTTGTTGAACCATCAATAATTAATCATTCTAAAGGCCACATAGTACCAAGACTTGGTAATATTTAATTTATTCATCTATCTATCAATCTATATACGTGCGTGTGATTTTCTTATGGTAATTTAGATATTTATATAATTTTATTTTGCAGATGAAAAAAGTTTGAAGATCGTTGACGGATTTATCCAAAAGGTTTCAAAGCTGTGACCACCTCAAACTAAAGAACACACATGGGTTTAAATAATTGAATCGAATTTACTTCTTAAATAAGCTTCCGAACTTTGTATCAAGAGTATATCTTAATTAAGTACGAGCATTTGAATTTTAAAATCCTAACTTTGTTGG

Coding sequence (CDS)

ATGGCAAGCGAGAGCAAAACTGAAAGGAAACCCAAGACTTTGTGCCTCCATGGCTTTCGGACTAGTGGAGCAATCTTGAGGAAGCAGGTCCAAAGATGGCCAACTTCTGTTCTTCATCAATTCCATCTCCATTTTATCGACGACTCAATCCCTTCCAAAGGAAAATCAGACTTCGAAGGAATTTACGATCCTCCCTATTTCGAGTGGTTTGGAACCAGCGAGGACCCGACCAATTATGAAAATTTGGAATCGAGCATTGAATTCATCGAGAGTTACATGCTTGAGCACGGACCATTCGACGGACTTCTCGGTTTTTCACAGGTAACTAAGCGTTGA

Protein sequence

MASESKTERKPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFEGIYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQVTKR

Homology

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BLAST of Cucsat.G4651.T9 vs. ExPASy Swiss-Prot
Match: S0EES1 (Esterase FUS5 OS=Gibberella fujikuroi (strain CBS 195.34 / IMI 58289 / NRRL A-6831) OX=1279085 GN=FUS5 PE=2 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 1.9e-08
Identity = 37/106 (34.91%), Postives = 57/106 (53.77%), Query Frame = 0


Query: 10  KPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFID---DSIPSKGKSDFEGIYDPPY 69
           +P+ LCLHG   S  I+R Q  +  +++   F L F++   DS P  G   F   +  PY
Sbjct: 4   RPRLLCLHGGGASSQIMRVQFSKLESALRKTFQLVFLEGPLDSAPGPGVLPFFEDFG-PY 63

Query: 70  FEWFGTS-----EDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQ 108
             W         E+    EN  ++I +I+++ML++GPF G+LGFSQ
Sbjct: 64  SCWVSDDKSLLPEEKRKEEN--NAIAYIKTFMLQYGPFAGILGFSQ 106

[Close]

BLAST of Cucsat.G4651.T9 vs. ExPASy Swiss-Prot
Match: W7MWX7 (Esterase FUS5 OS=Gibberella moniliformis (strain M3125 / FGSC 7600) OX=334819 GN=FUS5 PE=3 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 1.9e-08
Identity = 35/104 (33.65%), Postives = 56/104 (53.85%), Query Frame = 0


Query: 10  KPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFID---DSIPSKGKSDFEGIYDPPY 69
           +P+ LCLHG   S  I+R Q  +  +++   F L F++   DS P  G   F   +  PY
Sbjct: 4   RPRLLCLHGGGASSQIMRIQFSKLESALRKTFQLVFLEGPLDSAPGPGVLPFFKDFG-PY 63

Query: 70  FEWFGTSEDPTNYENLE---SSIEFIESYMLEHGPFDGLLGFSQ 108
             W       +  E  +   ++I +I+++ML++GPF G+LGFSQ
Sbjct: 64  SCWVSDDRSLSPEEKRQEETNAIAYIKTFMLQYGPFAGILGFSQ 106

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BLAST of Cucsat.G4651.T9 vs. ExPASy Swiss-Prot
Match: P36591 (Dihydrofolate reductase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dfr1 PE=2 SV=2)

HSP 1 Score: 58.9 bits (141), Expect = 4.1e-08
Identity = 34/108 (31.48%), Postives = 49/108 (45.37%), Query Frame = 0


Query: 12  KTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSD------------FE 71
           K LCLHG+  SG +  K++      +     LHF    + +  ++D              
Sbjct: 6   KVLCLHGWIQSGPVFSKKMGSVQKYLSKYAELHFPTGPVVADEEADPNDEEEKKRLAALG 65

Query: 72  GIYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQ 108
           G  +   F WF   +    Y + + S+E I  YM E GPFDGL+GFSQ
Sbjct: 66  GEQNGGKFGWFEVEDFKNTYGSWDESLECINQYMQEKGPFDGLIGFSQ 113

[Close]

BLAST of Cucsat.G4651.T9 vs. ExPASy Swiss-Prot
Match: C8VJR6 (Esterase alnB OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=alnB PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 5.1e-06
Identity = 30/97 (30.93%), Postives = 51/97 (52.58%), Query Frame = 0


Query: 11  PKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFEGIYDPPYFEWF 70
           PK LCLHG+ TS +IL+ Q+  +  +    +   F++  I  + K+   G +    F  +
Sbjct: 2   PKILCLHGYGTSASILQHQLGPFMAAADPSYEFVFLEGEIECQ-KAQGLGPFVKGPFLCY 61

Query: 71  GTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQ 108
             S  P    +++ S + I+  +   GPFDG++GFSQ
Sbjct: 62  NESFAPA---DIQESCDLIDEMIQAAGPFDGIIGFSQ 94

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BLAST of Cucsat.G4651.T9 vs. ExPASy Swiss-Prot
Match: Q99369 (Family of serine hydrolases 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=FSH3 PE=2 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 1.2e-04
Identity = 34/115 (29.57%), Postives = 53/115 (46.09%), Query Frame = 0


Query: 10  KPKTLCLHGFRTSGAI-------LRKQVQRWPTSVLHQFHLHFID-----DSIPSKGKS- 69
           K K L LHGF  S  I       LRK +++    + +    H ID      S   KG+  
Sbjct: 4   KKKVLMLHGFVQSDKIFSAKTGGLRKNLKKLGYDLYYPCAPHSIDKKALFQSESEKGRDA 63

Query: 70  ----DFEGIYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQ 108
               +     D  Y  +F   E   +++  +    ++ +Y+LE+GPFDG++GFSQ
Sbjct: 64  AKEFNTSATSDEVYGWFFRNPESFNSFQIDQKVFNYLRNYVLENGPFDGVIGFSQ 118

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BLAST of Cucsat.G4651.T9 vs. NCBI nr
Match: XP_004152615.3 (dihydrofolate reductase [Cucumis sativus])

HSP 1 Score: 223 bits (569), Expect = 5.21e-72
Identity = 105/107 (98.13%), Postives = 105/107 (98.13%), Query Frame = 0


Query: 1   MASESKTERKPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFEG 60
           MASESKTERKPK LCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSD EG
Sbjct: 1   MASESKTERKPKILCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDVEG 60

Query: 61  IYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQ 107
           IYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQ
Sbjct: 61  IYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQ 107

[Close]

BLAST of Cucsat.G4651.T9 vs. NCBI nr
Match: KGN62961.2 (hypothetical protein Csa_022631 [Cucumis sativus])

HSP 1 Score: 215 bits (548), Expect = 8.16e-69
Identity = 101/107 (94.39%), Postives = 103/107 (96.26%), Query Frame = 0


Query: 1   MASESKTERKPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFEG 60
           MASESKTERKPK LCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSD EG
Sbjct: 1   MASESKTERKPKILCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDVEG 60

Query: 61  IYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQ 107
           IYDPPYFEWFGTSEDPT+ ENLESS+EFIESYM EHGPFDGLLGFSQ
Sbjct: 61  IYDPPYFEWFGTSEDPTSCENLESSLEFIESYMAEHGPFDGLLGFSQ 107

[Close]

BLAST of Cucsat.G4651.T9 vs. NCBI nr
Match: XP_004152616.2 (esterase OVCA2 [Cucumis sativus])

HSP 1 Score: 203 bits (517), Expect = 4.22e-64
Identity = 94/107 (87.85%), Postives = 98/107 (91.59%), Query Frame = 0


Query: 1   MASESKTERKPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFEG 60
           MASE K ERKPK LCLHGFRTSGAILRKQVQRWPTS+LHQFHLHFID   PSKG+SD EG
Sbjct: 1   MASERKIERKPKILCLHGFRTSGAILRKQVQRWPTSILHQFHLHFIDGPFPSKGRSDVEG 60

Query: 61  IYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQ 107
           IYDPPYFEWFGTSEDPT+ ENLESS+EFIESYM EHGPFDGLLGFSQ
Sbjct: 61  IYDPPYFEWFGTSEDPTSCENLESSLEFIESYMAEHGPFDGLLGFSQ 107

[Close]

BLAST of Cucsat.G4651.T9 vs. NCBI nr
Match: XP_008444881.1 (PREDICTED: esterase OVCA2-like [Cucumis melo] >KAA0065117.1 esterase OVCA2-like [Cucumis melo var. makuwa])

HSP 1 Score: 194 bits (492), Expect = 2.65e-60
Identity = 87/107 (81.31%), Postives = 97/107 (90.65%), Query Frame = 0


Query: 1   MASESKTERKPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFEG 60
           M SESK ERKP  LCLHGFRTSGAILRKQ+Q+WPTSVLHQFH HFID  +PS+GKSD EG
Sbjct: 1   MTSESKIERKPTILCLHGFRTSGAILRKQIQKWPTSVLHQFHFHFIDGPLPSQGKSDVEG 60

Query: 61  IYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQ 107
           IYDPPYFEWFGTS+DPT+ ENLES +EFIE+YM++HGPFDGLLGFSQ
Sbjct: 61  IYDPPYFEWFGTSKDPTSCENLESCLEFIENYMVKHGPFDGLLGFSQ 107

[Close]

BLAST of Cucsat.G4651.T9 vs. NCBI nr
Match: XP_038884223.1 (dihydrofolate reductase-like isoform X2 [Benincasa hispida])

HSP 1 Score: 174 bits (440), Expect = 1.00e-52
Identity = 77/103 (74.76%), Postives = 87/103 (84.47%), Query Frame = 0


Query: 5   SKTERKPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFEGIYDP 64
           SK +RKPK LCLHGFRTSGAIL+KQ+++WP  VLHQFH HFID   PSKGKSD +GIYDP
Sbjct: 2   SKIQRKPKILCLHGFRTSGAILKKQIEKWPAFVLHQFHFHFIDAPFPSKGKSDVQGIYDP 61

Query: 65  PYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQ 107
           PYFEWF  S+D T YEN ES +EFIE++ML HGPFDGLLGFSQ
Sbjct: 62  PYFEWFQFSKDTTGYENFESCLEFIENHMLNHGPFDGLLGFSQ 104

[Close]

BLAST of Cucsat.G4651.T9 vs. ExPASy TrEMBL
Match: A0A0A0LPK3 (FSH1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G369780 PE=4 SV=1)

HSP 1 Score: 231 bits (589), Expect = 6.84e-77
Identity = 109/111 (98.20%), Postives = 109/111 (98.20%), Query Frame = 0


Query: 1   MASESKTERKPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFEG 60
           MASESKTERKPK LCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSD EG
Sbjct: 1   MASESKTERKPKILCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDVEG 60

Query: 61  IYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQVTKR 111
           IYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQVTKR
Sbjct: 61  IYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQVTKR 111

[Close]

BLAST of Cucsat.G4651.T9 vs. ExPASy TrEMBL
Match: A0A0A0LLR4 (FSH1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G369790 PE=4 SV=1)

HSP 1 Score: 203 bits (517), Expect = 6.44e-65
Identity = 94/107 (87.85%), Postives = 98/107 (91.59%), Query Frame = 0


Query: 1   MASESKTERKPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFEG 60
           MASE K ERKPK LCLHGFRTSGAILRKQVQRWPTS+LHQFHLHFID   PSKG+SD EG
Sbjct: 1   MASERKIERKPKILCLHGFRTSGAILRKQVQRWPTSILHQFHLHFIDGPFPSKGRSDVEG 60

Query: 61  IYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQ 107
           IYDPPYFEWFGTSEDPT+ ENLESS+EFIESYM EHGPFDGLLGFSQ
Sbjct: 61  IYDPPYFEWFGTSEDPTSCENLESSLEFIESYMAEHGPFDGLLGFSQ 107

[Close]

BLAST of Cucsat.G4651.T9 vs. ExPASy TrEMBL
Match: A0A5A7VDJ4 (Esterase OVCA2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004520 PE=4 SV=1)

HSP 1 Score: 194 bits (492), Expect = 1.29e-60
Identity = 87/107 (81.31%), Postives = 97/107 (90.65%), Query Frame = 0


Query: 1   MASESKTERKPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFEG 60
           M SESK ERKP  LCLHGFRTSGAILRKQ+Q+WPTSVLHQFH HFID  +PS+GKSD EG
Sbjct: 1   MTSESKIERKPTILCLHGFRTSGAILRKQIQKWPTSVLHQFHFHFIDGPLPSQGKSDVEG 60

Query: 61  IYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQ 107
           IYDPPYFEWFGTS+DPT+ ENLES +EFIE+YM++HGPFDGLLGFSQ
Sbjct: 61  IYDPPYFEWFGTSKDPTSCENLESCLEFIENYMVKHGPFDGLLGFSQ 107

[Close]

BLAST of Cucsat.G4651.T9 vs. ExPASy TrEMBL
Match: A0A1S3BC58 (esterase OVCA2-like OS=Cucumis melo OX=3656 GN=LOC103488096 PE=4 SV=1)

HSP 1 Score: 194 bits (492), Expect = 1.29e-60
Identity = 87/107 (81.31%), Postives = 97/107 (90.65%), Query Frame = 0


Query: 1   MASESKTERKPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFEG 60
           M SESK ERKP  LCLHGFRTSGAILRKQ+Q+WPTSVLHQFH HFID  +PS+GKSD EG
Sbjct: 1   MTSESKIERKPTILCLHGFRTSGAILRKQIQKWPTSVLHQFHFHFIDGPLPSQGKSDVEG 60

Query: 61  IYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQ 107
           IYDPPYFEWFGTS+DPT+ ENLES +EFIE+YM++HGPFDGLLGFSQ
Sbjct: 61  IYDPPYFEWFGTSKDPTSCENLESCLEFIENYMVKHGPFDGLLGFSQ 107

[Close]

BLAST of Cucsat.G4651.T9 vs. ExPASy TrEMBL
Match: A0A6J1HDC8 (dihydrofolate reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111462485 PE=4 SV=1)

HSP 1 Score: 160 bits (405), Expect = 2.05e-47
Identity = 70/107 (65.42%), Postives = 87/107 (81.31%), Query Frame = 0


Query: 1   MASESKTERKPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFEG 60
           M SES  +RKP+ LCLHGFRTSGAIL+K V++WPTSVL QF  HF+D   PS+GKS+ EG
Sbjct: 1   MGSESNIQRKPRILCLHGFRTSGAILKKHVEKWPTSVLDQFDFHFMDAPFPSRGKSEVEG 60

Query: 61  IYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQ 107
           ++DPPYFEWF   ED  ++ N+E  ++FIE++MLEHGPFDGLLGFSQ
Sbjct: 61  LHDPPYFEWFQFHEDTADFANVEECLQFIENHMLEHGPFDGLLGFSQ 107

[Close]

BLAST of Cucsat.G4651.T9 vs. TAIR 10
Match: AT4G24380.1 (INVOLVED IN: 10-formyltetrahydrofolate biosynthetic process, folic acid and derivative biosynthetic process; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 129.8 bits (325), Expect = 1.4e-30
Identity = 57/108 (52.78%), Postives = 79/108 (73.15%), Query Frame = 0


Query: 1   MASESKT-ERKPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFE 60
           M SE ++  RKP+ LCLHGFRTSG I++ Q+ +WP SV+ +  L F+D   P +GKSD E
Sbjct: 1   MGSEGRSIARKPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVE 60

Query: 61  GIYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQ 108
           GI+DPPY+EWF  +++ T Y N E  +E++E  M++ GPFDGL+GFSQ
Sbjct: 61  GIFDPPYYEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQ 108

[Close]

BLAST of Cucsat.G4651.T9 vs. TAIR 10
Match: AT4G24380.2 (CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 129.8 bits (325), Expect = 1.4e-30
Identity = 57/108 (52.78%), Postives = 79/108 (73.15%), Query Frame = 0


Query: 1   MASESKT-ERKPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFE 60
           M SE ++  RKP+ LCLHGFRTSG I++ Q+ +WP SV+ +  L F+D   P +GKSD E
Sbjct: 1   MGSEGRSIARKPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVE 60

Query: 61  GIYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQ 108
           GI+DPPY+EWF  +++ T Y N E  +E++E  M++ GPFDGL+GFSQ
Sbjct: 61  GIFDPPYYEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQ 108

[Close]

BLAST of Cucsat.G4651.T9 vs. TAIR 10
Match: AT5G65400.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 120.2 bits (300), Expect = 1.1e-27
Identity = 49/99 (49.49%), Postives = 68/99 (68.69%), Query Frame = 0


Query: 9   RKPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFEGIYDPPYFE 68
           + P+ LCLHGFRTSG IL+  + +WP ++L    L F+D   P+ GKSD E  +DPPY+E
Sbjct: 28  KNPRILCLHGFRTSGRILQAGIGKWPDTILRDLDLDFLDAPFPATGKSDVERFFDPPYYE 87

Query: 69  WFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQ 108
           W+  ++    Y N E  + +IE YM+++GPFDGLLGFSQ
Sbjct: 88  WYQANKGFKEYRNFEECLAYIEDYMIKNGPFDGLLGFSQ 126

[Close]

BLAST of Cucsat.G4651.T9 vs. TAIR 10
Match: AT1G09280.1 (CONTAINS InterPro DOMAIN/s: Rhodanese-like (InterPro:IPR001763), Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: Rhodanese/Cell cycle control phosphatase superfamily protein (TAIR:AT2G40760.1); Has 5925 Blast hits to 5912 proteins in 1592 species: Archae - 0; Bacteria - 2946; Metazoa - 156; Fungi - 408; Plants - 229; Viruses - 0; Other Eukaryotes - 2186 (source: NCBI BLink). )

HSP 1 Score: 47.0 bits (110), Expect = 1.2e-05
Identity = 37/130 (28.46%), Postives = 55/130 (42.31%), Query Frame = 0


Query: 7   TERKPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFEGIYD--- 66
           T RK + LCLHGFR + +  + +       + +   L FID         + + IY    
Sbjct: 350 TRRKLRILCLHGFRQNASSFKGRTGSLAKKLKNIAELVFID------APHELQFIYQTAT 409

Query: 67  PP------YFEWFGTSE----------------DPTNY----ENLESSIEFIESYMLEHG 108
           PP       F W  +S+                DP  Y    E  + S+ ++++   E G
Sbjct: 410 PPSGVCNKKFAWLVSSDFDKPSETGWTVAQCQFDPLQYQTQTEGFDKSLTYLKTAFEEKG 469

The following BLAST results are available for this feature:

BLAST of Cucsat.G4651.T9 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
S0EES1	1.9e-08	34.91	Esterase FUS5 OS=Gibberella fujikuroi (strain CBS 195.34 / IMI 58289 / NRRL A-68...	[more]
W7MWX7	1.9e-08	33.65	Esterase FUS5 OS=Gibberella moniliformis (strain M3125 / FGSC 7600) OX=334819 GN...	[more]
P36591	4.1e-08	31.48	Dihydrofolate reductase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) O...	[more]
C8VJR6	5.1e-06	30.93	Esterase alnB OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 /...	[more]
Q99369	1.2e-04	29.57	Family of serine hydrolases 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / ...	[more]

BLAST of Cucsat.G4651.T9 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004152615.3	5.21e-72	98.13	dihydrofolate reductase [Cucumis sativus]	[more]
KGN62961.2	8.16e-69	94.39	hypothetical protein Csa_022631 [Cucumis sativus]	[more]
XP_004152616.2	4.22e-64	87.85	esterase OVCA2 [Cucumis sativus]	[more]
XP_008444881.1	2.65e-60	81.31	PREDICTED: esterase OVCA2-like [Cucumis melo] >KAA0065117.1 esterase OVCA2-like ...	[more]
XP_038884223.1	1.00e-52	74.76	dihydrofolate reductase-like isoform X2 [Benincasa hispida]	[more]

BLAST of Cucsat.G4651.T9 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LPK3	6.84e-77	98.20	FSH1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G369780 PE=4 S...	[more]
A0A0A0LLR4	6.44e-65	87.85	FSH1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G369790 PE=4 S...	[more]
A0A5A7VDJ4	1.29e-60	81.31	Esterase OVCA2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G0...	[more]
A0A1S3BC58	1.29e-60	81.31	esterase OVCA2-like OS=Cucumis melo OX=3656 GN=LOC103488096 PE=4 SV=1	[more]
A0A6J1HDC8	2.05e-47	65.42	dihydrofolate reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111462485 PE=4 ...	[more]

BLAST of Cucsat.G4651.T9 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G24380.1	1.4e-30	52.78	INVOLVED IN: 10-formyltetrahydrofolate biosynthetic process, folic acid and deri...	[more]
AT4G24380.2	1.4e-30	52.78	CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidop...	[more]
AT5G65400.1	1.1e-27	49.49	alpha/beta-Hydrolases superfamily protein	[more]
AT1G09280.1	1.2e-05	28.46	CONTAINS InterPro DOMAIN/s: Rhodanese-like (InterPro:IPR001763), Serine hydrolas...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 6..109 e-value: 1.5E-22 score: 82.3
IPR005645	Serine hydrolase FSH	PFAM	PF03959	FSH1	coord: 9..107 e-value: 5.0E-21 score: 75.3
None	No IPR available	PANTHER	PTHR48070:SF5	DIHYDROFOLATE REDUCTASE	coord: 6..108
None	No IPR available	PANTHER	PTHR48070	ESTERASE OVCA2	coord: 6..108

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G4651	Cucsat.G4651	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G4651.T9.E1	Cucsat.G4651.T9.E1	exon
Cucsat.G4651.T9.E2	Cucsat.G4651.T9.E2	exon
Cucsat.G4651.T9.E3	Cucsat.G4651.T9.E3	exon
Cucsat.G4651.T9.E4	Cucsat.G4651.T9.E4	exon
Cucsat.G4651.T9.E5	Cucsat.G4651.T9.E5	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G4651.T9.C1	Cucsat.G4651.T9.C1	CDS
Cucsat.G4651.T9.C2	Cucsat.G4651.T9.C2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G4651.T9	Cucsat.G4651.T9-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus