Cucsat.G4651.T23 (mRNA) Cucumber (B10) v3

BLAST of Cucsat.G4651.T23 vs. ExPASy Swiss-Prot
Match: P36591 (Dihydrofolate reductase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dfr1 PE=2 SV=2)

HSP 1 Score: 70.9 bits (172), Expect = 1.7e-11
Identity = 50/167 (29.94%), Postives = 74/167 (44.31%), Query Frame = 0

Query: 12  KTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSD------------FE 71
           K LCLHG+  SG +  K++      +     LHF    + +  ++D              
Sbjct: 6   KVLCLHGWIQSGPVFSKKMGSVQKYLSKYAELHFPTGPVVADEEADPNDEEEKKRLAALG 65

Query: 72  GIYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQGAMLSAALALLQ 131
           G  +   F WF   +    Y + + S+E I  YM E GPFDGL+GFSQGA + A LA + 
Sbjct: 66  GEQNGGKFGWFEVEDFKNTYGSWDESLECINQYMQEKGPFDGLIGFSQGAGIGAMLAQML 125

Query: 132 ARGV---ALTKVPKIKFVIVISGSKLQSSSLAARIAYSTSIACPSLH 164
             G       + P  KFV+ + G + +         Y+  +  PSLH
Sbjct: 126 QPGQPPNPYVQHPPFKFVVFVGGFRAEKPEFDH--FYNPKLTTPSLH 170

BLAST of Cucsat.G4651.T23 vs. ExPASy Swiss-Prot
Match: S0EES1 (Esterase FUS5 OS=Gibberella fujikuroi (strain CBS 195.34 / IMI 58289 / NRRL A-6831) OX=1279085 GN=FUS5 PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.1e-10
Identity = 43/120 (35.83%), Postives = 66/120 (55.00%), Query Frame = 0

Query: 10  KPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFID---DSIPSKGKSDFEGIYDPPY 69
           +P+ LCLHG   S  I+R Q  +  +++   F L F++   DS P  G   F   +  PY
Sbjct: 4   RPRLLCLHGGGASSQIMRVQFSKLESALRKTFQLVFLEGPLDSAPGPGVLPFFEDFG-PY 63

Query: 70  FEWFGTS-----EDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQGAMLSAALALLQAR 122
             W         E+    EN  ++I +I+++ML++GPF G+LGFSQGA  +A++ L Q R
Sbjct: 64  SCWVSDDKSLLPEEKRKEEN--NAIAYIKTFMLQYGPFAGILGFSQGARATASILLEQQR 120

BLAST of Cucsat.G4651.T23 vs. ExPASy Swiss-Prot
Match: W7MWX7 (Esterase FUS5 OS=Gibberella moniliformis (strain M3125 / FGSC 7600) OX=334819 GN=FUS5 PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.1e-10
Identity = 41/118 (34.75%), Postives = 65/118 (55.08%), Query Frame = 0

Query: 10  KPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFID---DSIPSKGKSDFEGIYDPPY 69
           +P+ LCLHG   S  I+R Q  +  +++   F L F++   DS P  G   F   +  PY
Sbjct: 4   RPRLLCLHGGGASSQIMRIQFSKLESALRKTFQLVFLEGPLDSAPGPGVLPFFKDFG-PY 63

Query: 70  FEWFGTSEDPTNYENLE---SSIEFIESYMLEHGPFDGLLGFSQGAMLSAALALLQAR 122
             W       +  E  +   ++I +I+++ML++GPF G+LGFSQGA  +A++ L Q R
Sbjct: 64  SCWVSDDRSLSPEEKRQEETNAIAYIKTFMLQYGPFAGILGFSQGARAAASILLEQQR 120

BLAST of Cucsat.G4651.T23 vs. ExPASy Swiss-Prot
Match: Q99369 (Family of serine hydrolases 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=FSH3 PE=2 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 3.6e-09
Identity = 56/173 (32.37%), Postives = 79/173 (45.66%), Query Frame = 0

Query: 10  KPKTLCLHGFRTSGAI-------LRKQVQRWPTSVLHQFHLHFID-----DSIPSKGKS- 69
           K K L LHGF  S  I       LRK +++    + +    H ID      S   KG+  
Sbjct: 4   KKKVLMLHGFVQSDKIFSAKTGGLRKNLKKLGYDLYYPCAPHSIDKKALFQSESEKGRDA 63

Query: 70  ----DFEGIYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQGAMLS 129
               +     D  Y  +F   E   +++  +    ++ +Y+LE+GPFDG++GFSQGA L 
Sbjct: 64  AKEFNTSATSDEVYGWFFRNPESFNSFQIDQKVFNYLRNYVLENGPFDGVIGFSQGAGLG 123

Query: 130 AALALLQARGVALT--KVPKIKFVIVISGSKLQSSSLAARIAYSTSIACPSLH 164
             L     R + LT  + P +KF I  SG KL+  S      Y   I  PSLH
Sbjct: 124 GYLVTDFNRILNLTDEQQPALKFFISFSGFKLEDQSYQKE--YHRIIQVPSLH 174

BLAST of Cucsat.G4651.T23 vs. ExPASy Swiss-Prot
Match: Q94AC1 (Rhodanese-like domain-containing protein 6 OS=Arabidopsis thaliana OX=3702 GN=STR6 PE=2 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 3.4e-07
Identity = 54/192 (28.12%), Postives = 82/192 (42.71%), Query Frame = 0

Query: 7   TERKPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFEGIYD--- 66
           T RK + LCLHGFR + +  + +       + +   L FID         + + IY    
Sbjct: 350 TRRKLRILCLHGFRQNASSFKGRTGSLAKKLKNIAELVFID------APHELQFIYQTAT 409

Query: 67  PP------YFEWFGTSE----------------DPTNY----ENLESSIEFIESYMLEHG 126
           PP       F W  +S+                DP  Y    E  + S+ ++++   E G
Sbjct: 410 PPSGVCNKKFAWLVSSDFDKPSETGWTVAQCQFDPLQYQTQTEGFDKSLTYLKTAFEEKG 469

Query: 127 PFDGLLGFSQGAMLSAALALLQARGVALTKVPKIKFVIVISGSKLQSSSLAARIAYSTSI 170
           PFDG+LGFSQGA ++AA+   Q +   L      +F ++ SG    +      +    SI
Sbjct: 470 PFDGILGFSQGAAMAAAVCGKQEQ---LVGEIDFRFCVLCSGF---TPWPLLEMKEKRSI 529

BLAST of Cucsat.G4651.T23 vs. NCBI nr
Match: XP_004152615.3 (dihydrofolate reductase [Cucumis sativus])

HSP 1 Score: 321 bits (822), Expect = 2.74e-109
Identity = 162/167 (97.01%), Postives = 162/167 (97.01%), Query Frame = 0

Query: 1   MASESKTERKPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFEG 60
           MASESKTERKPK LCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSD EG
Sbjct: 1   MASESKTERKPKILCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDVEG 60

Query: 61  IYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQGAMLSAALALLQA 120
           IYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQGAMLSAALALLQA
Sbjct: 61  IYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQGAMLSAALALLQA 120

Query: 121 RGVALTKVPKIKFVIVISGSKLQSSSLAARIAYSTSIACPSLHFLSN 167
           RGVALTKVPKIKFVIVIS SKLQSSSLAARI YSTSIACPSLHFLS 
Sbjct: 121 RGVALTKVPKIKFVIVISSSKLQSSSLAARIVYSTSIACPSLHFLSE 167

BLAST of Cucsat.G4651.T23 vs. NCBI nr
Match: KGN62961.2 (hypothetical protein Csa_022631 [Cucumis sativus])

HSP 1 Score: 315 bits (807), Expect = 5.28e-107
Identity = 159/167 (95.21%), Postives = 162/167 (97.01%), Query Frame = 0

Query: 1   MASESKTERKPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFEG 60
           MASESKTERKPK LCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSD EG
Sbjct: 1   MASESKTERKPKILCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDVEG 60

Query: 61  IYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQGAMLSAALALLQA 120
           IYDPPYFEWFGTSEDPT+ ENLESS+EFIESYM EHGPFDGLLGFSQGA+LSAALALLQA
Sbjct: 61  IYDPPYFEWFGTSEDPTSCENLESSLEFIESYMAEHGPFDGLLGFSQGAVLSAALALLQA 120

Query: 121 RGVALTKVPKIKFVIVISGSKLQSSSLAARIAYSTSIACPSLHFLSN 167
           RGVALTKVPKIKFVIVISGSKLQSSSLAARIAYSTSIACPSLHFLS 
Sbjct: 121 RGVALTKVPKIKFVIVISGSKLQSSSLAARIAYSTSIACPSLHFLSE 167

BLAST of Cucsat.G4651.T23 vs. NCBI nr
Match: XP_004152616.2 (esterase OVCA2 [Cucumis sativus])

HSP 1 Score: 303 bits (776), Expect = 2.78e-102
Identity = 152/167 (91.02%), Postives = 157/167 (94.01%), Query Frame = 0

Query: 1   MASESKTERKPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFEG 60
           MASE K ERKPK LCLHGFRTSGAILRKQVQRWPTS+LHQFHLHFID   PSKG+SD EG
Sbjct: 1   MASERKIERKPKILCLHGFRTSGAILRKQVQRWPTSILHQFHLHFIDGPFPSKGRSDVEG 60

Query: 61  IYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQGAMLSAALALLQA 120
           IYDPPYFEWFGTSEDPT+ ENLESS+EFIESYM EHGPFDGLLGFSQGA+LSAALALLQA
Sbjct: 61  IYDPPYFEWFGTSEDPTSCENLESSLEFIESYMAEHGPFDGLLGFSQGAVLSAALALLQA 120

Query: 121 RGVALTKVPKIKFVIVISGSKLQSSSLAARIAYSTSIACPSLHFLSN 167
           RGVALTKVPKIKFVIVISGSKLQSSSLAARIAYSTSIACPSLHFLS 
Sbjct: 121 RGVALTKVPKIKFVIVISGSKLQSSSLAARIAYSTSIACPSLHFLSE 167

BLAST of Cucsat.G4651.T23 vs. NCBI nr
Match: XP_008444881.1 (PREDICTED: esterase OVCA2-like [Cucumis melo] >KAA0065117.1 esterase OVCA2-like [Cucumis melo var. makuwa])

HSP 1 Score: 285 bits (729), Expect = 3.98e-95
Identity = 141/167 (84.43%), Postives = 153/167 (91.62%), Query Frame = 0

Query: 1   MASESKTERKPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFEG 60
           M SESK ERKP  LCLHGFRTSGAILRKQ+Q+WPTSVLHQFH HFID  +PS+GKSD EG
Sbjct: 1   MTSESKIERKPTILCLHGFRTSGAILRKQIQKWPTSVLHQFHFHFIDGPLPSQGKSDVEG 60

Query: 61  IYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQGAMLSAALALLQA 120
           IYDPPYFEWFGTS+DPT+ ENLES +EFIE+YM++HGPFDGLLGFSQGA+LSAALA LQA
Sbjct: 61  IYDPPYFEWFGTSKDPTSCENLESCLEFIENYMVKHGPFDGLLGFSQGAVLSAALAGLQA 120

Query: 121 RGVALTKVPKIKFVIVISGSKLQSSSLAARIAYSTSIACPSLHFLSN 167
           RGVALTKVPKIKFVI+ISGSKLQSSSLAA IAYS SIACPSLHFLS 
Sbjct: 121 RGVALTKVPKIKFVIIISGSKLQSSSLAAGIAYSPSIACPSLHFLSE 167

BLAST of Cucsat.G4651.T23 vs. NCBI nr
Match: XP_038884223.1 (dihydrofolate reductase-like isoform X2 [Benincasa hispida])

HSP 1 Score: 253 bits (646), Expect = 7.59e-83
Identity = 125/164 (76.22%), Postives = 138/164 (84.15%), Query Frame = 0

Query: 5   SKTERKPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFEGIYDP 64
           SK +RKPK LCLHGFRTSGAIL+KQ+++WP  VLHQFH HFID   PSKGKSD +GIYDP
Sbjct: 2   SKIQRKPKILCLHGFRTSGAILKKQIEKWPAFVLHQFHFHFIDAPFPSKGKSDVQGIYDP 61

Query: 65  PYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQGAMLSAALALLQARGVA 124
           PYFEWF  S+D T YEN ES +EFIE++ML HGPFDGLLGFSQGA L+AAL  LQA+GVA
Sbjct: 62  PYFEWFQFSKDTTGYENFESCLEFIENHMLNHGPFDGLLGFSQGAALTAALPGLQAKGVA 121

Query: 125 LTKVPKIKFVIVISGSKLQSSSLAARIAYSTSIACPSLHFLSNF 168
           L KVPKIKFVI+ISGSKL S SLAA  AY  SIACPSLHFLSNF
Sbjct: 122 LRKVPKIKFVIIISGSKLGSPSLAAEKAYFPSIACPSLHFLSNF 165

BLAST of Cucsat.G4651.T23 vs. ExPASy TrEMBL
Match: A0A0A0LLR4 (FSH1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G369790 PE=4 SV=1)

HSP 1 Score: 329 bits (843), Expect = 2.38e-113
Identity = 167/181 (92.27%), Postives = 172/181 (95.03%), Query Frame = 0

Query: 1   MASESKTERKPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFEG 60
           MASE K ERKPK LCLHGFRTSGAILRKQVQRWPTS+LHQFHLHFID   PSKG+SD EG
Sbjct: 1   MASERKIERKPKILCLHGFRTSGAILRKQVQRWPTSILHQFHLHFIDGPFPSKGRSDVEG 60

Query: 61  IYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQGAMLSAALALLQA 120
           IYDPPYFEWFGTSEDPT+ ENLESS+EFIESYM EHGPFDGLLGFSQGA+LSAALALLQA
Sbjct: 61  IYDPPYFEWFGTSEDPTSCENLESSLEFIESYMAEHGPFDGLLGFSQGAVLSAALALLQA 120

Query: 121 RGVALTKVPKIKFVIVISGSKLQSSSLAARIAYSTSIACPSLHFLSNFPASSSFSFYIFS 180
           RGVALTKVPKIKFVIVISGSKLQSSSLAARIAYSTSIACPSLHFLSNFPASSSFSFYIFS
Sbjct: 121 RGVALTKVPKIKFVIVISGSKLQSSSLAARIAYSTSIACPSLHFLSNFPASSSFSFYIFS 180

BLAST of Cucsat.G4651.T23 vs. ExPASy TrEMBL
Match: A0A5A7VDJ4 (Esterase OVCA2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004520 PE=4 SV=1)

HSP 1 Score: 285 bits (729), Expect = 1.93e-95
Identity = 141/167 (84.43%), Postives = 153/167 (91.62%), Query Frame = 0

Query: 1   MASESKTERKPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFEG 60
           M SESK ERKP  LCLHGFRTSGAILRKQ+Q+WPTSVLHQFH HFID  +PS+GKSD EG
Sbjct: 1   MTSESKIERKPTILCLHGFRTSGAILRKQIQKWPTSVLHQFHFHFIDGPLPSQGKSDVEG 60

Query: 61  IYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQGAMLSAALALLQA 120
           IYDPPYFEWFGTS+DPT+ ENLES +EFIE+YM++HGPFDGLLGFSQGA+LSAALA LQA
Sbjct: 61  IYDPPYFEWFGTSKDPTSCENLESCLEFIENYMVKHGPFDGLLGFSQGAVLSAALAGLQA 120

Query: 121 RGVALTKVPKIKFVIVISGSKLQSSSLAARIAYSTSIACPSLHFLSN 167
           RGVALTKVPKIKFVI+ISGSKLQSSSLAA IAYS SIACPSLHFLS 
Sbjct: 121 RGVALTKVPKIKFVIIISGSKLQSSSLAAGIAYSPSIACPSLHFLSE 167

BLAST of Cucsat.G4651.T23 vs. ExPASy TrEMBL
Match: A0A1S3BC58 (esterase OVCA2-like OS=Cucumis melo OX=3656 GN=LOC103488096 PE=4 SV=1)

HSP 1 Score: 285 bits (729), Expect = 1.93e-95
Identity = 141/167 (84.43%), Postives = 153/167 (91.62%), Query Frame = 0

Query: 1   MASESKTERKPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFEG 60
           M SESK ERKP  LCLHGFRTSGAILRKQ+Q+WPTSVLHQFH HFID  +PS+GKSD EG
Sbjct: 1   MTSESKIERKPTILCLHGFRTSGAILRKQIQKWPTSVLHQFHFHFIDGPLPSQGKSDVEG 60

Query: 61  IYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQGAMLSAALALLQA 120
           IYDPPYFEWFGTS+DPT+ ENLES +EFIE+YM++HGPFDGLLGFSQGA+LSAALA LQA
Sbjct: 61  IYDPPYFEWFGTSKDPTSCENLESCLEFIENYMVKHGPFDGLLGFSQGAVLSAALAGLQA 120

Query: 121 RGVALTKVPKIKFVIVISGSKLQSSSLAARIAYSTSIACPSLHFLSN 167
           RGVALTKVPKIKFVI+ISGSKLQSSSLAA IAYS SIACPSLHFLS 
Sbjct: 121 RGVALTKVPKIKFVIIISGSKLQSSSLAAGIAYSPSIACPSLHFLSE 167

BLAST of Cucsat.G4651.T23 vs. ExPASy TrEMBL
Match: A0A6J1K941 (dihydrofolate reductase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492222 PE=4 SV=1)

HSP 1 Score: 230 bits (586), Expect = 1.09e-73
Identity = 109/167 (65.27%), Postives = 139/167 (83.23%), Query Frame = 0

Query: 1   MASESKTERKPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFEG 60
           M S+S+ +RKP+ LCLHGFRTSGAIL+K V++WPTSVL+QF  HF+D   PS+GKS+ EG
Sbjct: 1   MGSKSRIQRKPRILCLHGFRTSGAILKKHVEKWPTSVLNQFDFHFMDAPFPSRGKSEVEG 60

Query: 61  IYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQGAMLSAALALLQA 120
           ++DPPY+EWF   ED  ++ N+E  ++FIE++MLEHGPFDGLLGFSQGA L+A L   QA
Sbjct: 61  LHDPPYYEWFQFHEDTADFTNVEECLQFIENHMLEHGPFDGLLGFSQGAALAALLPGFQA 120

Query: 121 RGVALTKVPKIKFVIVISGSKLQSSSLAARIAYSTSIACPSLHFLSN 167
           +GVALT+VPKIKFVI+I+G KL+S SL A+ AY++SIACPSLHFLS 
Sbjct: 121 KGVALTRVPKIKFVIIINGVKLESPSLDAQEAYASSIACPSLHFLSE 167

BLAST of Cucsat.G4651.T23 vs. ExPASy TrEMBL
Match: A0A6J1HDC8 (dihydrofolate reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111462485 PE=4 SV=1)

HSP 1 Score: 229 bits (583), Expect = 3.10e-73
Identity = 111/167 (66.47%), Postives = 136/167 (81.44%), Query Frame = 0

Query: 1   MASESKTERKPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFEG 60
           M SES  +RKP+ LCLHGFRTSGAIL+K V++WPTSVL QF  HF+D   PS+GKS+ EG
Sbjct: 1   MGSESNIQRKPRILCLHGFRTSGAILKKHVEKWPTSVLDQFDFHFMDAPFPSRGKSEVEG 60

Query: 61  IYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQGAMLSAALALLQA 120
           ++DPPYFEWF   ED  ++ N+E  ++FIE++MLEHGPFDGLLGFSQGA L+A L   QA
Sbjct: 61  LHDPPYFEWFQFHEDTADFANVEECLQFIENHMLEHGPFDGLLGFSQGAALAALLPGFQA 120

Query: 121 RGVALTKVPKIKFVIVISGSKLQSSSLAARIAYSTSIACPSLHFLSN 167
           +GVALT+VPKIKFVI+ISG KL+S SL A+ A ++SIACPSLHFLS 
Sbjct: 121 KGVALTRVPKIKFVIIISGVKLESPSLDAQEASASSIACPSLHFLSE 167

BLAST of Cucsat.G4651.T23 vs. TAIR 10
Match: AT4G24380.2 (CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 186.4 bits (472), Expect = 2.0e-47
Identity = 93/169 (55.03%), Postives = 124/169 (73.37%), Query Frame = 0

Query: 1   MASESKT-ERKPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFE 60
           M SE ++  RKP+ LCLHGFRTSG I++ Q+ +WP SV+ +  L F+D   P +GKSD E
Sbjct: 1   MGSEGRSIARKPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVE 60

Query: 61  GIYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQGAMLSAALALLQ 120
           GI+DPPY+EWF  +++ T Y N E  +E++E  M++ GPFDGL+GFSQGA+LS  L  LQ
Sbjct: 61  GIFDPPYYEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGLPGLQ 120

Query: 121 ARGVALTKVPKIKFVIVISGSKLQSSSLAARIAYSTSIACPSLHFLSNF 169
           A+G+A  KVPKIKFVI+I G+KL+S+ LA   AYS+S+   SLHFL  F
Sbjct: 121 AKGIAFQKVPKIKFVIIIGGAKLKSAKLAEN-AYSSSLETLSLHFLGTF 168

BLAST of Cucsat.G4651.T23 vs. TAIR 10
Match: AT4G24380.1 (INVOLVED IN: 10-formyltetrahydrofolate biosynthetic process, folic acid and derivative biosynthetic process; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 184.5 bits (467), Expect = 7.6e-47
Identity = 92/166 (55.42%), Postives = 123/166 (74.10%), Query Frame = 0

Query: 1   MASESKT-ERKPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFE 60
           M SE ++  RKP+ LCLHGFRTSG I++ Q+ +WP SV+ +  L F+D   P +GKSD E
Sbjct: 1   MGSEGRSIARKPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVE 60

Query: 61  GIYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQGAMLSAALALLQ 120
           GI+DPPY+EWF  +++ T Y N E  +E++E  M++ GPFDGL+GFSQGA+LS  L  LQ
Sbjct: 61  GIFDPPYYEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGLPGLQ 120

Query: 121 ARGVALTKVPKIKFVIVISGSKLQSSSLAARIAYSTSIACPSLHFL 166
           A+G+A  KVPKIKFVI+I G+KL+S+ LA   AYS+S+   SLHFL
Sbjct: 121 AKGIAFQKVPKIKFVIIIGGAKLKSAKLAEN-AYSSSLETLSLHFL 165

BLAST of Cucsat.G4651.T23 vs. TAIR 10
Match: AT5G65400.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 169.9 bits (429), Expect = 1.9e-42
Identity = 79/161 (49.07%), Postives = 112/161 (69.57%), Query Frame = 0

Query: 9   RKPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFEGIYDPPYFE 68
           + P+ LCLHGFRTSG IL+  + +WP ++L    L F+D   P+ GKSD E  +DPPY+E
Sbjct: 28  KNPRILCLHGFRTSGRILQAGIGKWPDTILRDLDLDFLDAPFPATGKSDVERFFDPPYYE 87

Query: 69  WFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQGAMLSAALALLQARGVALTKV 128
           W+  ++    Y N E  + +IE YM+++GPFDGLLGFSQGA L+AA+  +Q +G ALTKV
Sbjct: 88  WYQANKGFKEYRNFEECLAYIEDYMIKNGPFDGLLGFSQGAFLTAAIPGMQEQGSALTKV 147

Query: 129 PKIKFVIVISGSKLQ----SSSLAARIAYSTSIACPSLHFL 166
           PK+KF+++ISG+K+         AA  A+S+ + CPSLHF+
Sbjct: 148 PKVKFLVIISGAKIPGLMFGEPKAAVNAFSSPVRCPSLHFI 188

BLAST of Cucsat.G4651.T23 vs. TAIR 10
Match: AT1G09280.1 (CONTAINS InterPro DOMAIN/s: Rhodanese-like (InterPro:IPR001763), Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: Rhodanese/Cell cycle control phosphatase superfamily protein (TAIR:AT2G40760.1); Has 5925 Blast hits to 5912 proteins in 1592 species: Archae - 0; Bacteria - 2946; Metazoa - 156; Fungi - 408; Plants - 229; Viruses - 0; Other Eukaryotes - 2186 (source: NCBI BLink). )

HSP 1 Score: 56.6 bits (135), Expect = 2.4e-08
Identity = 54/192 (28.12%), Postives = 82/192 (42.71%), Query Frame = 0

Query: 7   TERKPKTLCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDFEGIYD--- 66
           T RK + LCLHGFR + +  + +       + +   L FID         + + IY    
Sbjct: 350 TRRKLRILCLHGFRQNASSFKGRTGSLAKKLKNIAELVFID------APHELQFIYQTAT 409

Query: 67  PP------YFEWFGTSE----------------DPTNY----ENLESSIEFIESYMLEHG 126
           PP       F W  +S+                DP  Y    E  + S+ ++++   E G
Sbjct: 410 PPSGVCNKKFAWLVSSDFDKPSETGWTVAQCQFDPLQYQTQTEGFDKSLTYLKTAFEEKG 469

Query: 127 PFDGLLGFSQGAMLSAALALLQARGVALTKVPKIKFVIVISGSKLQSSSLAARIAYSTSI 170
           PFDG+LGFSQGA ++AA+   Q +   L      +F ++ SG    +      +    SI
Sbjct: 470 PFDGILGFSQGAAMAAAVCGKQEQ---LVGEIDFRFCVLCSGF---TPWPLLEMKEKRSI 529