Cucsat.G4359.T12 (mRNA) Cucumber (B10) v3

BLAST of Cucsat.G4359.T12 vs. ExPASy Swiss-Prot
Match: P42534 (Putative polyketide hydroxylase OS=Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) OX=100226 GN=SCO5321 PE=3 SV=2)

HSP 1 Score: 162.5 bits (410), Expect = 1.4e-38
Identity = 156/545 (28.62%), Postives = 234/545 (42.94%), Query Frame = 0

Query: 38  VCSPDSLEGPCVVREKK----ILLGHECVSIDATDESVNMTASYLKEGKHVERRNISCNI 97
           +CS + LE   +         +  G E +S +A  E V       + G+H     I  + 
Sbjct: 128 LCSQNDLEPELLTHATNLGGDLRFGTELLSFEADTEGVTAIVKSRETGEHT---TIRADY 187

Query: 98  LVGADGAGSTVRRLVGIEMKGENDLQKLVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIG 157
           LV ADG  S VR  +GI   G  DL   VSI F SR L + ++ DR  ++ ++ +  A G
Sbjct: 188 LVAADGPRSPVREQLGIGQSGPGDLFHNVSITFRSRRLAD-VVGDRRFIVCYLTDENADG 247

Query: 158 VLVAHDLKQGEFILQVPFYPPQ-QNIEDFFPQMCEELIFKLVGRNLCDIDVRDVKPWIMH 217
            L+  D ++  ++   P++P Q + +EDF  + C   I + +G    D+++    PW   
Sbjct: 248 ALLPVDNRE-NWVFHAPWHPEQGETVEDFTDERCAAHIRRAIGDPDLDVEITGKAPWHAA 307

Query: 218 AEVAEKFICRQNHVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILN 277
             VA  +  R   VLLAGD+AH   P G FG NTGIQD HNLAWKLAAVL+  A  ++L+
Sbjct: 308 QRVARSY--RSGRVLLAGDSAHEMSPTGAFGSNTGIQDAHNLAWKLAAVLEGWAGEALLD 367

Query: 278 TYEMERRPIALFNTALSVKNFKAAMEVPAALGLDPKIANSVHRVVNHGLGSILSSSLQSA 337
           TY+ ERRP+A    A S +    ++E        P +A         G G+   +   + 
Sbjct: 368 TYDTERRPVA---EATSARAAHRSVEHSHPGFAPPPVAGG----GGPGAGTPGGAGRGTG 427

Query: 338 VLDGIFKIGRLQLSDTFLNVENPIGSSRLAKLRHIFDEGKS-------------LQLQFP 397
              G    G L           P G             G +              Q    
Sbjct: 428 GPGGPGGPGGLGGPGGPGGTGGPGGPGGPGGPDGPRGAGGAPGGGPGGGPGGGGPQRGIL 487

Query: 398 AEDLGFRYSEGAIIRDNNLLGGREEPTGRRRQYLP-SADPGSRLPHMNVRVLASEEIIST 457
              LG+RY  GA++       G +  T    + L  +  PGSR PH+ VR    ++ +ST
Sbjct: 488 NVALGYRYPRGAVV-------GADPATPVVPEGLDLTGAPGSRAPHLWVR--RGQDRLST 547

Query: 458 LDLVSGDKIEFLLIIAPRSESYRLAHAALKVAEEFKTSVKVCILWSANTTKIESSSKDQL 517
           LDL      + L++++  ++      AA  VA   +  +K        + ++  S    L
Sbjct: 548 LDLYE----DSLVLLSDAAQPTGWHEAAAGVAAGMRVPLK--------SYRVGGSPGADL 607

Query: 518 TPWENYIEVQEIRQSITSPSWWDVCKMTDKGAILVRPDEHIAWRVKSGISGDPNTELIGV 564
            P +                W     +T  GA+LVRPD  +AWR   G + DP + L  V
Sbjct: 608 NPDDE------------ETDWARAHGVTRGGAVLVRPDGFVAWR-SPGPAPDPESMLRQV 624

BLAST of Cucsat.G4359.T12 vs. ExPASy Swiss-Prot
Match: Q05355 (Putative polyketide hydroxylase OS=Streptomyces halstedii OX=1944 GN=schC PE=3 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 8.2e-36
Identity = 93/254 (36.61%), Postives = 136/254 (53.54%), Query Frame = 0

Query: 38  VCSPDSLEGPCVVREKKILLGHE---CVSIDATDESVNMTASYLKEGKHVERRNISCNIL 97
           +CS + LE   V+ +  + LG E      + + ++      + +K  +  E   +  + L
Sbjct: 122 LCSQNDLEP--VLLDHAVELGGEIRFSTELQSFEQDPAGVTAVIKSRRSGEHTTVRADYL 181

Query: 98  VGADGAGSTVRRLVGIEMKGENDLQKLVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGV 157
           V ADG  S VR  +GI   G  DL   VS+ F SR L E+ + DR  ++ ++ N EA G 
Sbjct: 182 VAADGPRSPVREQLGIGQSGPGDLFHNVSVTFRSRRLAEF-VGDRHFIVCYLTNPEADGA 241

Query: 158 LVAHDLKQGEFILQVPFYP-PQQNIEDFFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHA 217
           L+  D ++  ++   P+YP   + +EDF  + C + I + VG    D+++    PW    
Sbjct: 242 LLPVDNRE-NWVFHAPWYPRAARPLEDFTDERCADHIRRAVGVPDLDVEITGKAPWHAAQ 301

Query: 218 EVAEKFICRQNHVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNT 277
            VA ++  R   V LAGD+AH   P G FG NTGIQD HNLAWKLAAVL   A   +L+T
Sbjct: 302 RVARQY--RAGRVFLAGDSAHEMSPTGAFGSNTGIQDAHNLAWKLAAVLGGWAGDGLLDT 361

Query: 278 YEMERRPIALFNTA 288
           Y+ ERRP+A   TA
Sbjct: 362 YDAERRPVAEATTA 369

BLAST of Cucsat.G4359.T12 vs. ExPASy Swiss-Prot
Match: P27138 (2,4-dichlorophenol 6-monooxygenase OS=Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) OX=264198 GN=tfdB PE=3 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 1.5e-29
Identity = 141/558 (25.27%), Postives = 228/558 (40.86%), Query Frame = 0

Query: 11  SPVSVAHFSQYKLNGLLLKQLQNLGFQVCSPDSLEGPCVVREKKILLGHECVSIDATDES 70
           SP S+    Q  L  +L+K             +L+G C VR     LGHE        + 
Sbjct: 111 SPTSMCDLPQNYLEPILVKSA-----------ALDG-CKVRFDTEYLGHE--------QD 170

Query: 71  VNMTASYLKEGKHVERRNISCNILVGADGAGSTVRRLVGIEMKGENDLQKLVSIH-FFSR 130
            +  +S L++  + E   +    L+GADGA S V  +  +++  E  + K  SI+  F  
Sbjct: 171 ADGVSSRLRDRLNGEEFTVRSKYLIGADGANSRV--VSDLDLPLEGTMGKSGSINLLFEA 230

Query: 131 ELGEYLLKDRPGMLYFIF----NTEAIGVLVAHDLKQGEFILQVPFYPPQQNIEDFFPQM 190
           +L  Y +  RP +LY++     +   +G+ V   ++     L +  Y  +Q   +     
Sbjct: 231 DLDRY-VAHRPSVLYWVIQPGSDIGGLGIGVVRMVRPWNKWLAIWGYDVEQGPPEISESF 290

Query: 191 CEELIFKLVGRNLCDIDVRDVKPWIMHAEVAEKFICRQNHVLLAGDAAHRFPPAGGFGMN 250
              ++  L+G +   + +  +  W ++   A +   +Q  V  AGDA HR PP  G G N
Sbjct: 291 ARRIVHNLIGDDSVPLKIEGISTWTVNDMYATRL--QQGRVFCAGDAVHRHPPTNGLGSN 350

Query: 251 TGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIALFNTALSVKNFKAAMEVPAALGL 310
           T IQD  NLAWK+A VL   A  S+L+TY +ER PIA      + K+ +    +  ALGL
Sbjct: 351 TSIQDSFNLAWKIAMVLNGTADESLLDTYTIERAPIAKQVVCRANKSLEDFPPIAMALGL 410

Query: 311 DPKIANSVHRVVNHGLGSILSSSLQSAVLDGIFKIGRLQLSDTFLNVENPIGSSRLAKLR 370
            P+  ++            + S++      G                  P   ++  +LR
Sbjct: 411 -PQAKSA----------DEMKSNMARRKEPG------------------PEAQAQRTRLR 470

Query: 371 HIFDEGKSLQLQFPAEDLGFRYSEGAIIRDNNLLGGREEPTGRRRQYLPSADPGSRLPHM 430
                G +        ++  RY   AI+ DN+     E        +  S  PG+ +PH+
Sbjct: 471 EAI-AGTNYVYNAHGVEMNQRYDSPAIVADNS---PDEVFRDVELYHQASTRPGAPMPHV 530

Query: 431 NVRVLASEEIISTLDLVSGDKIEFLLIIAPRSESYRLAHAALKVAEEFKTSVKVCILWSA 490
            V        IST DL    K  F L       +++ A AA  V+ +   +V V I+   
Sbjct: 531 WVYASGDGHRISTKDLCG--KGNFTLFTGIGGAAWQDAAAA--VSRQLGVAVTVRIIGPG 581

Query: 491 NTTKIESSSKDQLTPWENYIEVQEIRQSITSPSWWDVCKMTDKGAILVRPDEHIAWRVKS 550
              +            ++Y +   I + I            D GAILVRPD H+A+R  S
Sbjct: 591 QAYE------------DHYGDFARISEII------------DTGAILVRPDFHVAYRATS 581

Query: 551 GISGDPNTELIGVFTTLL 564
            +  D   +L+     +L
Sbjct: 651 -LPADAAGDLVSAMRRIL 581

BLAST of Cucsat.G4359.T12 vs. ExPASy Swiss-Prot
Match: P39888 (Tetracenomycin polyketide synthesis hydroxylase TcmG OS=Streptomyces glaucescens OX=1907 GN=tcmG PE=3 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 1.8e-27
Identity = 85/255 (33.33%), Postives = 127/255 (49.80%), Query Frame = 0

Query: 39  CSPDSLEGPCVVREKKILLG-----HECVSIDATDESVNMTASYLKEGKHVERRNISCNI 98
           C  D LE   ++R++ +  G     +  +     DES  +TA+ + +    E+  +    
Sbjct: 137 CGQDRLEP--ILRDEAVRRGARIDFNTRMEAFTADES-GVTATIVDQATG-EQSTVRARY 196

Query: 99  LVGADGAGSTVRRLVGIEMKGENDLQKLVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIG 158
           L+ ADG  S VR  +GI   G   +   +S+  F  +L +  +K R  ++ ++ N +  G
Sbjct: 197 LIAADGVRSPVRETLGITRTGHGTIGNAMSV-LFKADLRD-TVKGRRFVICYLPNPDEPG 256

Query: 159 VL-------VAHDLKQGEFILQVPFYPPQQNIEDFFPQMCEELIFKLVGRNLCDIDVRDV 218
           VL       V     +  +I    F P + + E F  + C ++I    G     ++V+  
Sbjct: 257 VLQLPEVPAVLQLFDEDRWIFGFFFDPRETSPEQFTDERCAQIIRTATGLPGLPVEVQMA 316

Query: 219 KPWIMHAEVAEKFICRQNHVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIA 278
           +PW M    A  +  R   V LAGDAAH  PPAG FG N GIQD HNLAWKLAAVL+  A
Sbjct: 317 RPWEMSHNSARSY--RSGRVFLAGDAAHVHPPAGAFGANGGIQDAHNLAWKLAAVLKGTA 376

Query: 279 SPSILNTYEMERRPI 282
             ++L+TYE ER PI
Sbjct: 377 GDALLDTYEQERLPI 383

BLAST of Cucsat.G4359.T12 vs. ExPASy Swiss-Prot
Match: P31020 (Phenol 2-monooxygenase OS=Pseudomonas sp. (strain EST1001) OX=69012 GN=pheA PE=3 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 4.2e-24
Identity = 110/394 (27.92%), Postives = 157/394 (39.85%), Query Frame = 0

Query: 173 PPQQNIEDFFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEVAEKFICRQNHVLLAGDA 232
           PP+ N ++       +++  LVG    D+++     W  + + A     ++  V  AGDA
Sbjct: 278 PPKLNDDEAI-----QIVRNLVGIEDLDVEILGYSLWGNNDQYATHL--QKGRVCCAGDA 337

Query: 233 AHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIALFNTALSVKN 292
            H+ PP+ G G NT IQD +NL WKLA VL+  A P +L TY  ER PIA          
Sbjct: 338 IHKHPPSHGLGSNTSIQDSYNLCWKLACVLKGQAGPELLETYSTERAPIA---------- 397

Query: 293 FKAAMEVPAALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLDGIFKIGRLQLSDTF--- 352
                                 ++V    G   SSS    + D +  +     +D F   
Sbjct: 398 ---------------------KQIVTRANG---SSSEYKPIFDAL-GVTDATTNDEFVEK 457

Query: 353 --LNVEN-PIGSSRLAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRDNNLLGGREEPT 412
             L  EN P G+ R A LR   D  K  +      ++G  Y   A+I D        E  
Sbjct: 458 LALRKENSPEGARRRAALRAALD-NKDYEFNAQGTEIGQFYDSSAVITDGQKRPAMTEDP 517

Query: 413 GRRRQYLPSADPGSRLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAPRSESYRLAHAA 472
               Q   S  PG RLPH    +  ++E  ST D+  G +      I  ++     A AA
Sbjct: 518 MLHHQ--KSTFPGLRLPH--AWLGDAKEKYSTHDIAEGTRFTIFTGITGQA----WADAA 577

Query: 473 LKVAEEFKTSVKVCILWSANTTKIESSSKDQLTPWENYIEVQEIRQSITSPSWWDVCKMT 532
           ++VAE     +K  ++            +D    W    EV E                 
Sbjct: 578 VRVAERLGIDLKAVVIGEGQPV------QDLYGDWLRQREVDE----------------- 595

Query: 533 DKGAILVRPDEHIAWRVKSGISGDPNTELIGVFT 561
             G ILVRPD+HI WR +S ++ DP T L  V +
Sbjct: 638 -DGVILVRPDKHIGWRAQSMVA-DPETALFDVLS 595

BLAST of Cucsat.G4359.T12 vs. NCBI nr
Match: KAE8648228.1 (hypothetical protein Csa_018353 [Cucumis sativus])

HSP 1 Score: 1134 bits (2934), Expect = 0.0
Identity = 564/564 (100.00%), Postives = 564/564 (100.00%), Query Frame = 0

Query: 1    MQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLGFQVCSPDSLEGPCVVREKKILLGHE 60
            MQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLGFQVCSPDSLEGPCVVREKKILLGHE
Sbjct: 451  MQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLGFQVCSPDSLEGPCVVREKKILLGHE 510

Query: 61   CVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADGAGSTVRRLVGIEMKGENDLQK 120
            CVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADGAGSTVRRLVGIEMKGENDLQK
Sbjct: 511  CVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADGAGSTVRRLVGIEMKGENDLQK 570

Query: 121  LVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQNIED 180
            LVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQNIED
Sbjct: 571  LVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQNIED 630

Query: 181  FFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEVAEKFICRQNHVLLAGDAAHRFPPAG 240
            FFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEVAEKFICRQNHVLLAGDAAHRFPPAG
Sbjct: 631  FFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEVAEKFICRQNHVLLAGDAAHRFPPAG 690

Query: 241  GFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIALFNTALSVKNFKAAMEVP 300
            GFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIALFNTALSVKNFKAAMEVP
Sbjct: 691  GFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIALFNTALSVKNFKAAMEVP 750

Query: 301  AALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLDGIFKIGRLQLSDTFLNVENPIGSSR 360
            AALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLDGIFKIGRLQLSDTFLNVENPIGSSR
Sbjct: 751  AALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLDGIFKIGRLQLSDTFLNVENPIGSSR 810

Query: 361  LAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRDNNLLGGREEPTGRRRQYLPSADPGS 420
            LAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRDNNLLGGREEPTGRRRQYLPSADPGS
Sbjct: 811  LAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRDNNLLGGREEPTGRRRQYLPSADPGS 870

Query: 421  RLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAPRSESYRLAHAALKVAEEFKTSVKVC 480
            RLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAPRSESYRLAHAALKVAEEFKTSVKVC
Sbjct: 871  RLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAPRSESYRLAHAALKVAEEFKTSVKVC 930

Query: 481  ILWSANTTKIESSSKDQLTPWENYIEVQEIRQSITSPSWWDVCKMTDKGAILVRPDEHIA 540
            ILWSANTTKIESSSKDQLTPWENYIEVQEIRQSITSPSWWDVCKMTDKGAILVRPDEHIA
Sbjct: 931  ILWSANTTKIESSSKDQLTPWENYIEVQEIRQSITSPSWWDVCKMTDKGAILVRPDEHIA 990

Query: 541  WRVKSGISGDPNTELIGVFTTLLK 564
            WRVKSGISGDPNTELIGVFTTLLK
Sbjct: 991  WRVKSGISGDPNTELIGVFTTLLK 1014

BLAST of Cucsat.G4359.T12 vs. NCBI nr
Match: XP_004142293.1 (uncharacterized protein LOC101210766 [Cucumis sativus] >XP_031742115.1 uncharacterized protein LOC101210766 [Cucumis sativus])

HSP 1 Score: 1134 bits (2934), Expect = 0.0
Identity = 564/564 (100.00%), Postives = 564/564 (100.00%), Query Frame = 0

Query: 1   MQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLGFQVCSPDSLEGPCVVREKKILLGHE 60
           MQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLGFQVCSPDSLEGPCVVREKKILLGHE
Sbjct: 146 MQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLGFQVCSPDSLEGPCVVREKKILLGHE 205

Query: 61  CVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADGAGSTVRRLVGIEMKGENDLQK 120
           CVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADGAGSTVRRLVGIEMKGENDLQK
Sbjct: 206 CVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADGAGSTVRRLVGIEMKGENDLQK 265

Query: 121 LVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQNIED 180
           LVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQNIED
Sbjct: 266 LVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQNIED 325

Query: 181 FFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEVAEKFICRQNHVLLAGDAAHRFPPAG 240
           FFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEVAEKFICRQNHVLLAGDAAHRFPPAG
Sbjct: 326 FFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEVAEKFICRQNHVLLAGDAAHRFPPAG 385

Query: 241 GFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIALFNTALSVKNFKAAMEVP 300
           GFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIALFNTALSVKNFKAAMEVP
Sbjct: 386 GFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIALFNTALSVKNFKAAMEVP 445

Query: 301 AALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLDGIFKIGRLQLSDTFLNVENPIGSSR 360
           AALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLDGIFKIGRLQLSDTFLNVENPIGSSR
Sbjct: 446 AALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLDGIFKIGRLQLSDTFLNVENPIGSSR 505

Query: 361 LAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRDNNLLGGREEPTGRRRQYLPSADPGS 420
           LAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRDNNLLGGREEPTGRRRQYLPSADPGS
Sbjct: 506 LAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRDNNLLGGREEPTGRRRQYLPSADPGS 565

Query: 421 RLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAPRSESYRLAHAALKVAEEFKTSVKVC 480
           RLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAPRSESYRLAHAALKVAEEFKTSVKVC
Sbjct: 566 RLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAPRSESYRLAHAALKVAEEFKTSVKVC 625

Query: 481 ILWSANTTKIESSSKDQLTPWENYIEVQEIRQSITSPSWWDVCKMTDKGAILVRPDEHIA 540
           ILWSANTTKIESSSKDQLTPWENYIEVQEIRQSITSPSWWDVCKMTDKGAILVRPDEHIA
Sbjct: 626 ILWSANTTKIESSSKDQLTPWENYIEVQEIRQSITSPSWWDVCKMTDKGAILVRPDEHIA 685

Query: 541 WRVKSGISGDPNTELIGVFTTLLK 564
           WRVKSGISGDPNTELIGVFTTLLK
Sbjct: 686 WRVKSGISGDPNTELIGVFTTLLK 709

BLAST of Cucsat.G4359.T12 vs. NCBI nr
Match: XP_008454726.1 (PREDICTED: putative polyketide hydroxylase [Cucumis melo] >XP_008454728.1 PREDICTED: putative polyketide hydroxylase [Cucumis melo])

HSP 1 Score: 1078 bits (2787), Expect = 0.0
Identity = 534/564 (94.68%), Postives = 549/564 (97.34%), Query Frame = 0

Query: 1   MQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLGFQVCSPDSLEGPCVVREKKILLGHE 60
           MQPQDFEHIISPVSVAHFSQYKLN LLLKQLQNLGFQV SPDSLEGPCVVREKKILLGHE
Sbjct: 146 MQPQDFEHIISPVSVAHFSQYKLNRLLLKQLQNLGFQVGSPDSLEGPCVVREKKILLGHE 205

Query: 61  CVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADGAGSTVRRLVGIEMKGENDLQK 120
           CVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADGAGSTVRRLVG+EMKGENDLQK
Sbjct: 206 CVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADGAGSTVRRLVGVEMKGENDLQK 265

Query: 121 LVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQNIED 180
           LVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQNIED
Sbjct: 266 LVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQNIED 325

Query: 181 FFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEVAEKFICRQNHVLLAGDAAHRFPPAG 240
           F P MC ELIFKLVG+NLCDIDV+DVKPWIMHAEVAEKFIC +NHVLLAGDAAHRFPPAG
Sbjct: 326 FCPAMCNELIFKLVGQNLCDIDVQDVKPWIMHAEVAEKFICCRNHVLLAGDAAHRFPPAG 385

Query: 241 GFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIALFNTALSVKNFKAAMEVP 300
           GFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIALFNTALSVKNFKAAMEVP
Sbjct: 386 GFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIALFNTALSVKNFKAAMEVP 445

Query: 301 AALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLDGIFKIGRLQLSDTFLNVENPIGSSR 360
           AALGLDPKIANSVHRVVN+GLGSILSSSLQSAVLDGIFKIGRLQLSD FLNV+NPIGSSR
Sbjct: 446 AALGLDPKIANSVHRVVNNGLGSILSSSLQSAVLDGIFKIGRLQLSDIFLNVKNPIGSSR 505

Query: 361 LAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRDNNLLGGREEPTGRRRQYLPSADPGS 420
           LAKLRHIFDEGKSLQLQFPAEDLGFRYS+GAII DN LLGGREEPTGRRRQY+PSADPGS
Sbjct: 506 LAKLRHIFDEGKSLQLQFPAEDLGFRYSDGAIIPDNTLLGGREEPTGRRRQYIPSADPGS 565

Query: 421 RLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAPRSESYRLAHAALKVAEEFKTSVKVC 480
           RLPHMNVRVLASE+IISTLDLVSGDKIEFLLIIAPRSESY LAHA  KVAEEFKTSVKVC
Sbjct: 566 RLPHMNVRVLASEDIISTLDLVSGDKIEFLLIIAPRSESYHLAHAGFKVAEEFKTSVKVC 625

Query: 481 ILWSANTTKIESSSKDQLTPWENYIEVQEIRQSITSPSWWDVCKMTDKGAILVRPDEHIA 540
           ILWSA+TTKIESSSKD LTPWENY++V+EIRQS TSPSWWD+CKMTDKGAILVRPDEHIA
Sbjct: 626 ILWSASTTKIESSSKDLLTPWENYVDVEEIRQSTTSPSWWDICKMTDKGAILVRPDEHIA 685

Query: 541 WRVKSGISGDPNTELIGVFTTLLK 564
           WRVKSGISGDPNTEL+ VFTTLLK
Sbjct: 686 WRVKSGISGDPNTELMRVFTTLLK 709

BLAST of Cucsat.G4359.T12 vs. NCBI nr
Match: XP_038892398.1 (4-methyl-5-nitrocatechol 5-monooxygenase isoform X2 [Benincasa hispida] >XP_038892399.1 4-methyl-5-nitrocatechol 5-monooxygenase isoform X2 [Benincasa hispida] >XP_038892400.1 4-methyl-5-nitrocatechol 5-monooxygenase isoform X2 [Benincasa hispida])

HSP 1 Score: 1038 bits (2684), Expect = 0.0
Identity = 510/564 (90.43%), Postives = 540/564 (95.74%), Query Frame = 0

Query: 1   MQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLGFQVCSPDSLEGPCVVREKKILLGHE 60
           MQPQDFEHI SPVSVAHFSQYKLN LLLKQLQNLGFQVC+P+SLEGPCVVREKKILLGHE
Sbjct: 1   MQPQDFEHITSPVSVAHFSQYKLNRLLLKQLQNLGFQVCTPESLEGPCVVREKKILLGHE 60

Query: 61  CVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADGAGSTVRRLVGIEMKGENDLQK 120
           CVSIDATDE++ MTASYLKEG H++RRNISCN+LVGADGAGS+VRRLVGIEMKGENDLQK
Sbjct: 61  CVSIDATDETITMTASYLKEGTHIKRRNISCNVLVGADGAGSSVRRLVGIEMKGENDLQK 120

Query: 121 LVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQNIED 180
           LVS+HFFSRELGEYLLK+RPGMLYF+FNTEAIGVLVAHDLKQGEFILQVPFYPPQQNI D
Sbjct: 121 LVSVHFFSRELGEYLLKERPGMLYFVFNTEAIGVLVAHDLKQGEFILQVPFYPPQQNIGD 180

Query: 181 FFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEVAEKFICRQNHVLLAGDAAHRFPPAG 240
           F P+MCEELIFKLVG+NLCDIDV+DVKPWIMHAEVAEKFIC Q+ VLLAGDAAHRFPPAG
Sbjct: 181 FCPKMCEELIFKLVGQNLCDIDVQDVKPWIMHAEVAEKFICCQSRVLLAGDAAHRFPPAG 240

Query: 241 GFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIALFNTALSVKNFKAAMEVP 300
           GFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIALFNTALSVKNFKAAMEVP
Sbjct: 241 GFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIALFNTALSVKNFKAAMEVP 300

Query: 301 AALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLDGIFKIGRLQLSDTFLNVENPIGSSR 360
           AALGLDPKIANSVHRVVNHGLGSILSSS QSAVLDGIFKIGRLQLSD+FLN  NP+GSSR
Sbjct: 301 AALGLDPKIANSVHRVVNHGLGSILSSSQQSAVLDGIFKIGRLQLSDSFLNDGNPVGSSR 360

Query: 361 LAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRDNNLLGGREEPTGRRRQYLPSADPGS 420
           LAKLR IFDEGKSLQLQFPAEDLGFRYSEGAII D++LLGGREEPTGRRRQY+PSADPGS
Sbjct: 361 LAKLRQIFDEGKSLQLQFPAEDLGFRYSEGAIIPDDSLLGGREEPTGRRRQYVPSADPGS 420

Query: 421 RLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAPRSESYRLAHAALKVAEEFKTSVKVC 480
           RLPHMNVRVLASEEI STLDLVSGD ++FLLII PR ESY LAHA LKVAEEFK SV+VC
Sbjct: 421 RLPHMNVRVLASEEIFSTLDLVSGDVVQFLLIIGPRPESYCLAHATLKVAEEFKISVRVC 480

Query: 481 ILWSANTTKIESSSKDQLTPWENYIEVQEIRQSITSPSWWDVCKMTDKGAILVRPDEHIA 540
           ILWSA+TTKI+SSSK++LTPW+NYI+VQEIRQ  TSPSWWDVC+MTDKGAILVRPDEHIA
Sbjct: 481 ILWSADTTKIQSSSKEELTPWKNYIDVQEIRQWSTSPSWWDVCQMTDKGAILVRPDEHIA 540

Query: 541 WRVKSGISGDPNTELIGVFTTLLK 564
           WRVKSGISGDPNTEL  VFTTLLK
Sbjct: 541 WRVKSGISGDPNTELTRVFTTLLK 564

BLAST of Cucsat.G4359.T12 vs. NCBI nr
Match: XP_038892396.1 (2,4-dichlorophenol 6-monooxygenase isoform X1 [Benincasa hispida] >XP_038892397.1 2,4-dichlorophenol 6-monooxygenase isoform X1 [Benincasa hispida])

HSP 1 Score: 1038 bits (2684), Expect = 0.0
Identity = 510/564 (90.43%), Postives = 540/564 (95.74%), Query Frame = 0

Query: 1   MQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLGFQVCSPDSLEGPCVVREKKILLGHE 60
           MQPQDFEHI SPVSVAHFSQYKLN LLLKQLQNLGFQVC+P+SLEGPCVVREKKILLGHE
Sbjct: 138 MQPQDFEHITSPVSVAHFSQYKLNRLLLKQLQNLGFQVCTPESLEGPCVVREKKILLGHE 197

Query: 61  CVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADGAGSTVRRLVGIEMKGENDLQK 120
           CVSIDATDE++ MTASYLKEG H++RRNISCN+LVGADGAGS+VRRLVGIEMKGENDLQK
Sbjct: 198 CVSIDATDETITMTASYLKEGTHIKRRNISCNVLVGADGAGSSVRRLVGIEMKGENDLQK 257

Query: 121 LVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQNIED 180
           LVS+HFFSRELGEYLLK+RPGMLYF+FNTEAIGVLVAHDLKQGEFILQVPFYPPQQNI D
Sbjct: 258 LVSVHFFSRELGEYLLKERPGMLYFVFNTEAIGVLVAHDLKQGEFILQVPFYPPQQNIGD 317

Query: 181 FFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEVAEKFICRQNHVLLAGDAAHRFPPAG 240
           F P+MCEELIFKLVG+NLCDIDV+DVKPWIMHAEVAEKFIC Q+ VLLAGDAAHRFPPAG
Sbjct: 318 FCPKMCEELIFKLVGQNLCDIDVQDVKPWIMHAEVAEKFICCQSRVLLAGDAAHRFPPAG 377

Query: 241 GFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIALFNTALSVKNFKAAMEVP 300
           GFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIALFNTALSVKNFKAAMEVP
Sbjct: 378 GFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIALFNTALSVKNFKAAMEVP 437

Query: 301 AALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLDGIFKIGRLQLSDTFLNVENPIGSSR 360
           AALGLDPKIANSVHRVVNHGLGSILSSS QSAVLDGIFKIGRLQLSD+FLN  NP+GSSR
Sbjct: 438 AALGLDPKIANSVHRVVNHGLGSILSSSQQSAVLDGIFKIGRLQLSDSFLNDGNPVGSSR 497

Query: 361 LAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRDNNLLGGREEPTGRRRQYLPSADPGS 420
           LAKLR IFDEGKSLQLQFPAEDLGFRYSEGAII D++LLGGREEPTGRRRQY+PSADPGS
Sbjct: 498 LAKLRQIFDEGKSLQLQFPAEDLGFRYSEGAIIPDDSLLGGREEPTGRRRQYVPSADPGS 557

Query: 421 RLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAPRSESYRLAHAALKVAEEFKTSVKVC 480
           RLPHMNVRVLASEEI STLDLVSGD ++FLLII PR ESY LAHA LKVAEEFK SV+VC
Sbjct: 558 RLPHMNVRVLASEEIFSTLDLVSGDVVQFLLIIGPRPESYCLAHATLKVAEEFKISVRVC 617

Query: 481 ILWSANTTKIESSSKDQLTPWENYIEVQEIRQSITSPSWWDVCKMTDKGAILVRPDEHIA 540
           ILWSA+TTKI+SSSK++LTPW+NYI+VQEIRQ  TSPSWWDVC+MTDKGAILVRPDEHIA
Sbjct: 618 ILWSADTTKIQSSSKEELTPWKNYIDVQEIRQWSTSPSWWDVCQMTDKGAILVRPDEHIA 677

Query: 541 WRVKSGISGDPNTELIGVFTTLLK 564
           WRVKSGISGDPNTEL  VFTTLLK
Sbjct: 678 WRVKSGISGDPNTELTRVFTTLLK 701

BLAST of Cucsat.G4359.T12 vs. ExPASy TrEMBL
Match: A0A0A0KLD9 (FAD_binding_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G056620 PE=4 SV=1)

HSP 1 Score: 1134 bits (2934), Expect = 0.0
Identity = 564/564 (100.00%), Postives = 564/564 (100.00%), Query Frame = 0

Query: 1   MQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLGFQVCSPDSLEGPCVVREKKILLGHE 60
           MQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLGFQVCSPDSLEGPCVVREKKILLGHE
Sbjct: 138 MQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLGFQVCSPDSLEGPCVVREKKILLGHE 197

Query: 61  CVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADGAGSTVRRLVGIEMKGENDLQK 120
           CVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADGAGSTVRRLVGIEMKGENDLQK
Sbjct: 198 CVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADGAGSTVRRLVGIEMKGENDLQK 257

Query: 121 LVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQNIED 180
           LVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQNIED
Sbjct: 258 LVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQNIED 317

Query: 181 FFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEVAEKFICRQNHVLLAGDAAHRFPPAG 240
           FFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEVAEKFICRQNHVLLAGDAAHRFPPAG
Sbjct: 318 FFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEVAEKFICRQNHVLLAGDAAHRFPPAG 377

Query: 241 GFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIALFNTALSVKNFKAAMEVP 300
           GFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIALFNTALSVKNFKAAMEVP
Sbjct: 378 GFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIALFNTALSVKNFKAAMEVP 437

Query: 301 AALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLDGIFKIGRLQLSDTFLNVENPIGSSR 360
           AALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLDGIFKIGRLQLSDTFLNVENPIGSSR
Sbjct: 438 AALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLDGIFKIGRLQLSDTFLNVENPIGSSR 497

Query: 361 LAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRDNNLLGGREEPTGRRRQYLPSADPGS 420
           LAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRDNNLLGGREEPTGRRRQYLPSADPGS
Sbjct: 498 LAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRDNNLLGGREEPTGRRRQYLPSADPGS 557

Query: 421 RLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAPRSESYRLAHAALKVAEEFKTSVKVC 480
           RLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAPRSESYRLAHAALKVAEEFKTSVKVC
Sbjct: 558 RLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAPRSESYRLAHAALKVAEEFKTSVKVC 617

Query: 481 ILWSANTTKIESSSKDQLTPWENYIEVQEIRQSITSPSWWDVCKMTDKGAILVRPDEHIA 540
           ILWSANTTKIESSSKDQLTPWENYIEVQEIRQSITSPSWWDVCKMTDKGAILVRPDEHIA
Sbjct: 618 ILWSANTTKIESSSKDQLTPWENYIEVQEIRQSITSPSWWDVCKMTDKGAILVRPDEHIA 677

Query: 541 WRVKSGISGDPNTELIGVFTTLLK 564
           WRVKSGISGDPNTELIGVFTTLLK
Sbjct: 678 WRVKSGISGDPNTELIGVFTTLLK 701

BLAST of Cucsat.G4359.T12 vs. ExPASy TrEMBL
Match: A0A1S3BZD5 (putative polyketide hydroxylase OS=Cucumis melo OX=3656 GN=LOC103495067 PE=4 SV=1)

HSP 1 Score: 1078 bits (2787), Expect = 0.0
Identity = 534/564 (94.68%), Postives = 549/564 (97.34%), Query Frame = 0

Query: 1   MQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLGFQVCSPDSLEGPCVVREKKILLGHE 60
           MQPQDFEHIISPVSVAHFSQYKLN LLLKQLQNLGFQV SPDSLEGPCVVREKKILLGHE
Sbjct: 146 MQPQDFEHIISPVSVAHFSQYKLNRLLLKQLQNLGFQVGSPDSLEGPCVVREKKILLGHE 205

Query: 61  CVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADGAGSTVRRLVGIEMKGENDLQK 120
           CVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADGAGSTVRRLVG+EMKGENDLQK
Sbjct: 206 CVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADGAGSTVRRLVGVEMKGENDLQK 265

Query: 121 LVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQNIED 180
           LVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQNIED
Sbjct: 266 LVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQNIED 325

Query: 181 FFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEVAEKFICRQNHVLLAGDAAHRFPPAG 240
           F P MC ELIFKLVG+NLCDIDV+DVKPWIMHAEVAEKFIC +NHVLLAGDAAHRFPPAG
Sbjct: 326 FCPAMCNELIFKLVGQNLCDIDVQDVKPWIMHAEVAEKFICCRNHVLLAGDAAHRFPPAG 385

Query: 241 GFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIALFNTALSVKNFKAAMEVP 300
           GFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIALFNTALSVKNFKAAMEVP
Sbjct: 386 GFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIALFNTALSVKNFKAAMEVP 445

Query: 301 AALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLDGIFKIGRLQLSDTFLNVENPIGSSR 360
           AALGLDPKIANSVHRVVN+GLGSILSSSLQSAVLDGIFKIGRLQLSD FLNV+NPIGSSR
Sbjct: 446 AALGLDPKIANSVHRVVNNGLGSILSSSLQSAVLDGIFKIGRLQLSDIFLNVKNPIGSSR 505

Query: 361 LAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRDNNLLGGREEPTGRRRQYLPSADPGS 420
           LAKLRHIFDEGKSLQLQFPAEDLGFRYS+GAII DN LLGGREEPTGRRRQY+PSADPGS
Sbjct: 506 LAKLRHIFDEGKSLQLQFPAEDLGFRYSDGAIIPDNTLLGGREEPTGRRRQYIPSADPGS 565

Query: 421 RLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAPRSESYRLAHAALKVAEEFKTSVKVC 480
           RLPHMNVRVLASE+IISTLDLVSGDKIEFLLIIAPRSESY LAHA  KVAEEFKTSVKVC
Sbjct: 566 RLPHMNVRVLASEDIISTLDLVSGDKIEFLLIIAPRSESYHLAHAGFKVAEEFKTSVKVC 625

Query: 481 ILWSANTTKIESSSKDQLTPWENYIEVQEIRQSITSPSWWDVCKMTDKGAILVRPDEHIA 540
           ILWSA+TTKIESSSKD LTPWENY++V+EIRQS TSPSWWD+CKMTDKGAILVRPDEHIA
Sbjct: 626 ILWSASTTKIESSSKDLLTPWENYVDVEEIRQSTTSPSWWDICKMTDKGAILVRPDEHIA 685

Query: 541 WRVKSGISGDPNTELIGVFTTLLK 564
           WRVKSGISGDPNTEL+ VFTTLLK
Sbjct: 686 WRVKSGISGDPNTELMRVFTTLLK 709

BLAST of Cucsat.G4359.T12 vs. ExPASy TrEMBL
Match: A0A6J1HUM9 (uncharacterized protein LOC111466353 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466353 PE=4 SV=1)

HSP 1 Score: 999 bits (2584), Expect = 0.0
Identity = 492/565 (87.08%), Postives = 525/565 (92.92%), Query Frame = 0

Query: 1   MQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLGFQVCSPDSLEGPCVVREKKILLGHE 60
           MQPQDFEHIISPVSVAHFSQYKLN LLLKQLQNLGFQVCSPDS EGPC+VREK+IL+GHE
Sbjct: 138 MQPQDFEHIISPVSVAHFSQYKLNRLLLKQLQNLGFQVCSPDSSEGPCIVREKQILMGHE 197

Query: 61  CVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADGAGSTVRRLVGIEMKGENDLQK 120
           CVSI  TD++V MTASYLKEGKH+ERRNI  NILVGADGAGSTVRRLVGIEMKGE +LQK
Sbjct: 198 CVSIGVTDDAVTMTASYLKEGKHIERRNICSNILVGADGAGSTVRRLVGIEMKGEKNLQK 257

Query: 121 LVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQNIED 180
           LVSIHFFSRELGEYLL +RPGMLYFIFN EAIGVLVAHDLKQGEFILQVPFYPPQQNIED
Sbjct: 258 LVSIHFFSRELGEYLLTERPGMLYFIFNIEAIGVLVAHDLKQGEFILQVPFYPPQQNIED 317

Query: 181 FFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEVAEKFICRQNHVLLAGDAAHRFPPAG 240
           F P+MCEE+IF LVG NLCD+DV+DVKPWIMHAEVAEKFI  QN VLLAGDAAHRFPPAG
Sbjct: 318 FCPKMCEEIIFNLVGLNLCDVDVQDVKPWIMHAEVAEKFIACQNRVLLAGDAAHRFPPAG 377

Query: 241 GFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIALFNTALSVKNFKAAMEVP 300
           GFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMER+PIAL NTALSVKNFKAAMEVP
Sbjct: 378 GFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERKPIALLNTALSVKNFKAAMEVP 437

Query: 301 AALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLDGIFKIGRLQLSDTFLNVENPIGSSR 360
           AALGLDPKIANSVH+ VNHGLGS+LSSSLQ  VLDGIFKIGRLQLSDT LN +NP+GSSR
Sbjct: 438 AALGLDPKIANSVHQAVNHGLGSVLSSSLQRTVLDGIFKIGRLQLSDTLLNEKNPVGSSR 497

Query: 361 LAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRDNNLLGGREEPTGRRRQYLPSADPGS 420
           LAKL HIFDEGKSLQLQFPAEDLGFRYSEGA+I DNN LGGREEPTGRRR+Y+PSADPGS
Sbjct: 498 LAKLSHIFDEGKSLQLQFPAEDLGFRYSEGALIPDNNQLGGREEPTGRRRRYIPSADPGS 557

Query: 421 RLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAPRSESYRLAHAALKVAEEFKTSVKVC 480
           +LPHMNVR LAS E+ISTLDLVSGDK+EFLLIIAP  E YRLA AAL VAEEFKTSV+VC
Sbjct: 558 KLPHMNVRALASMEVISTLDLVSGDKVEFLLIIAPLPEFYRLARAALMVAEEFKTSVRVC 617

Query: 481 ILWSANTTKIESSSKDQLTPWENYIEVQEIRQSITSP-SWWDVCKMTDKGAILVRPDEHI 540
           ILWSA+ T+IESSSK++L PWENYI+VQEIRQ  TS  SWWDVC+MTDKGAILVRPDEH+
Sbjct: 618 ILWSADITRIESSSKEELAPWENYIDVQEIRQPSTSTASWWDVCQMTDKGAILVRPDEHV 677

Query: 541 AWRVKSGISGDPNTELIGVFTTLLK 564
           AWRVKSG+SGDPNTEL  VFT+LLK
Sbjct: 678 AWRVKSGVSGDPNTELRRVFTSLLK 702

BLAST of Cucsat.G4359.T12 vs. ExPASy TrEMBL
Match: A0A6J1G1R0 (uncharacterized protein LOC111449881 OS=Cucurbita moschata OX=3662 GN=LOC111449881 PE=4 SV=1)

HSP 1 Score: 998 bits (2579), Expect = 0.0
Identity = 492/565 (87.08%), Postives = 524/565 (92.74%), Query Frame = 0

Query: 1   MQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLGFQVCSPDSLEGPCVVREKKILLGHE 60
           MQPQDFEHIISPVSVAHFSQYKLN LLLKQLQNL FQVCSPDS EGPC+VREK+IL+GHE
Sbjct: 171 MQPQDFEHIISPVSVAHFSQYKLNRLLLKQLQNLEFQVCSPDSSEGPCIVREKQILMGHE 230

Query: 61  CVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADGAGSTVRRLVGIEMKGENDLQK 120
           CVSI  TD++V MTASYLKEGKH+ERRNI  NILVGADGAGSTVRRLVGIEMKGE DLQK
Sbjct: 231 CVSIGVTDDAVTMTASYLKEGKHIERRNICSNILVGADGAGSTVRRLVGIEMKGEKDLQK 290

Query: 121 LVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQNIED 180
           LVSIHFFSRELGEYLL +RPGMLYFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQNIED
Sbjct: 291 LVSIHFFSRELGEYLLTERPGMLYFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQNIED 350

Query: 181 FFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEVAEKFICRQNHVLLAGDAAHRFPPAG 240
           F P+MCEE+IF LVG NLCD+DV+DVKPWIMHAEVAEKFI  Q  VLLAGDAAHRFPPAG
Sbjct: 351 FCPKMCEEIIFDLVGLNLCDVDVQDVKPWIMHAEVAEKFISCQGRVLLAGDAAHRFPPAG 410

Query: 241 GFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIALFNTALSVKNFKAAMEVP 300
           GFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMER+PIAL NTALSVKNFKAAMEVP
Sbjct: 411 GFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERKPIALLNTALSVKNFKAAMEVP 470

Query: 301 AALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLDGIFKIGRLQLSDTFLNVENPIGSSR 360
           AALGLDPKIANSVH+ VNHGLGS+LSSSLQ  VLDGIFKIGRLQLSDT LN +NP+GSSR
Sbjct: 471 AALGLDPKIANSVHQAVNHGLGSVLSSSLQREVLDGIFKIGRLQLSDTLLNDKNPVGSSR 530

Query: 361 LAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRDNNLLGGREEPTGRRRQYLPSADPGS 420
           LAKL HIFDEGKSLQLQFPAEDLGFRYSEGA++ DNN  GGREEPTGRRR+Y+PSADPGS
Sbjct: 531 LAKLSHIFDEGKSLQLQFPAEDLGFRYSEGALLPDNNQPGGREEPTGRRRRYIPSADPGS 590

Query: 421 RLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAPRSESYRLAHAALKVAEEFKTSVKVC 480
           +LPHMNVR LAS E+ISTLDLVSGDK+EFLLIIAP  ESYRLA AAL VAEEFKTSV+VC
Sbjct: 591 KLPHMNVRALASMEVISTLDLVSGDKVEFLLIIAPLPESYRLARAALMVAEEFKTSVRVC 650

Query: 481 ILWSANTTKIESSSKDQLTPWENYIEVQEIRQSITSP-SWWDVCKMTDKGAILVRPDEHI 540
           ILWSA+ T+IESSSK++LTPWENYI+VQEIRQ  TS  SWWDVC+MTDKGAILVRPDEH+
Sbjct: 651 ILWSADITRIESSSKEELTPWENYIDVQEIRQPSTSTASWWDVCQMTDKGAILVRPDEHV 710

Query: 541 AWRVKSGISGDPNTELIGVFTTLLK 564
            WRVKSG+SGDPNTEL  VFTTLLK
Sbjct: 711 GWRVKSGVSGDPNTELRRVFTTLLK 735

BLAST of Cucsat.G4359.T12 vs. ExPASy TrEMBL
Match: A0A6J1DCK1 (uncharacterized protein LOC111019789 OS=Momordica charantia OX=3673 GN=LOC111019789 PE=4 SV=1)

HSP 1 Score: 985 bits (2547), Expect = 0.0
Identity = 485/565 (85.84%), Postives = 523/565 (92.57%), Query Frame = 0

Query: 1   MQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLGFQVCSPDSLEGPCVVREKKILLGHE 60
           MQPQDF +IISPVSVAHFSQYKLN LLLK+LQNLGFQVCSPDSLE  C+VREK+ILLGHE
Sbjct: 158 MQPQDFGNIISPVSVAHFSQYKLNRLLLKKLQNLGFQVCSPDSLEDTCIVREKQILLGHE 217

Query: 61  CVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADGAGSTVRRLVGIEMKGENDLQK 120
           CVSIDATD+SV  TASYLKEGKH ERRNI  NILVG DGAGSTVRRLVGIE+KGENDLQK
Sbjct: 218 CVSIDATDDSVTTTASYLKEGKHTERRNICSNILVGTDGAGSTVRRLVGIEIKGENDLQK 277

Query: 121 LVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQNIED 180
           LVS+HFFSRELGEYLL +RPGMLYFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQ+IED
Sbjct: 278 LVSVHFFSRELGEYLLNERPGMLYFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQSIED 337

Query: 181 FFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEVAEKFICRQNHVLLAGDAAHRFPPAG 240
           F P+MCEELIFKLVG NLCDIDV+DVKPWIMHAEVAEKFIC  N VLLAGDAAHRFPPAG
Sbjct: 338 FSPKMCEELIFKLVGLNLCDIDVQDVKPWIMHAEVAEKFICCHNRVLLAGDAAHRFPPAG 397

Query: 241 GFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIALFNTALSVKNFKAAMEVP 300
           GFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIA FNTALSVKNFKAAMEVP
Sbjct: 398 GFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIAQFNTALSVKNFKAAMEVP 457

Query: 301 AALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLDGIFKIGRLQLSDTFLNVENPIGSSR 360
           AALGLDP+IANSVH+ VNHGLGS+L SSLQS+VLDGIFKIGR+QLS++ LN +NPIGSSR
Sbjct: 458 AALGLDPRIANSVHQAVNHGLGSVLPSSLQSSVLDGIFKIGRMQLSESLLNDKNPIGSSR 517

Query: 361 LAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRDNNLLGGREEPTGRRRQYLPSADPGS 420
           LAKLR IFDEGKSLQLQFPAEDLGFRYSEGA+I DN LL G EEPTGRRRQY+PSADPGS
Sbjct: 518 LAKLRQIFDEGKSLQLQFPAEDLGFRYSEGALIPDNTLLSGPEEPTGRRRQYVPSADPGS 577

Query: 421 RLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAPRSESYRLAHAALKVA-EEFKTSVKV 480
           RLPHMNVR LASE++ISTLDLVSGDK+EFLLIIAP  ESY LA +ALK+A EEFKTSVKV
Sbjct: 578 RLPHMNVRTLASEDMISTLDLVSGDKVEFLLIIAPLPESYHLARSALKIAAEEFKTSVKV 637

Query: 481 CILWSANTTKIESSSKDQLTPWENYIEVQEIRQSITSPSWWDVCKMTDKGAILVRPDEHI 540
           C+LWSA+  ++ES S+ +LTPWENY++VQEIRQS TSPSWWDVC+MTD GAILVRPDEHI
Sbjct: 638 CVLWSADIPRVESRSQQELTPWENYMDVQEIRQSSTSPSWWDVCRMTDNGAILVRPDEHI 697

Query: 541 AWRVKSGISGDPNTELIGVFTTLLK 564
           AWRVKSG+SGD NT++  VFT LLK
Sbjct: 698 AWRVKSGVSGDLNTQMKRVFTALLK 722

BLAST of Cucsat.G4359.T12 vs. TAIR 10
Match: AT1G24340.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 756.5 bits (1952), Expect = 1.5e-218
Identity = 364/571 (63.75%), Postives = 454/571 (79.51%), Query Frame = 0

Query: 1   MQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLGFQV---CSPDSLEGPCVVREKKILL 60
           MQPQDFE ++SP SVAHFSQYKL  LLLK+L++LGF V      D LE   VV  ++IL+
Sbjct: 138 MQPQDFEKVVSPASVAHFSQYKLTNLLLKRLEDLGFHVRGSKESDGLEADSVV-ARQILM 197

Query: 61  GHECVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADGAGSTVRRLVGIEMKGEND 120
           GHECV IDA  +S+  T S+LK GKH+ +RNI C++LVGADGAGS VR+L  IEM+GE D
Sbjct: 198 GHECVGIDANKDSITATVSFLKGGKHM-KRNIQCSLLVGADGAGSAVRKLTVIEMRGERD 257

Query: 121 LQKLVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQN 180
           LQKLVS+HF SRELGEYL+ +RPGML+FIFNT+ IGVLVAHDL QGEF+LQ+P+YPPQQ+
Sbjct: 258 LQKLVSVHFMSRELGEYLISNRPGMLFFIFNTDGIGVLVAHDLLQGEFVLQIPYYPPQQS 317

Query: 181 IEDFFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEVAEKFICRQNHVLLAGDAAHRFP 240
           + DF P+MC+ LIF LVG  L D+DV D+KPW+MHAEVAEKF+C +N V+LAGDAAHRFP
Sbjct: 318 LSDFSPEMCKMLIFNLVGHELSDLDVADIKPWVMHAEVAEKFMCCENRVILAGDAAHRFP 377

Query: 241 PAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIALFNTALSVKNFKAAM 300
           PAGGFGMNTGIQD HNLAWK+AA++Q  A+ SIL TYE ERRPIAL NT+LSV+NF+AAM
Sbjct: 378 PAGGFGMNTGIQDAHNLAWKIAALVQGSANSSILKTYETERRPIALSNTSLSVQNFRAAM 437

Query: 301 EVPAALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLDGIFKIGRLQLSDTFLNVENPIG 360
            VP+ALGLDP +ANSVHR +N  +GSIL + LQ A+LD +F +GR QLS++ LN  NP+G
Sbjct: 438 SVPSALGLDPTVANSVHRFINKTVGSILPTGLQKAILDNVFALGRAQLSESLLNESNPLG 497

Query: 361 SSRLAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRDN-NLLGGREEPTGRRRQYLPSA 420
           + RL++L+ IF+ GKSLQLQFPAEDLGFRY EGAI+ DN +  G  E P+GRRR Y+P A
Sbjct: 498 NQRLSRLKSIFEGGKSLQLQFPAEDLGFRYLEGAIVPDNESEAGDPEVPSGRRRDYVPCA 557

Query: 421 DPGSRLPHMNVRVLAS---EEIISTLDLVSGDKIEFLLIIAPRSESYRLAHAALKVAEEF 480
           +PGSRLPHM V++L+    E I+STLDLVS +K+EFLLII+P  ESY LAHA  KVA+EF
Sbjct: 558 EPGSRLPHMYVKILSDSTREVIVSTLDLVSTEKVEFLLIISPLQESYELAHATFKVAKEF 617

Query: 481 KTSVKVCILWSANTTKIESSSKDQLTPWENYIEVQEI-RQSITSPSWWDVCKMTDKGAIL 540
             SVKVC++W ++   +E  S   L PWENY++V E+ +Q+    SWW +CKM+++G+IL
Sbjct: 618 MASVKVCVVWPSSDDGLERKSNSALAPWENYVDVMEVKKQNGEGTSWWSICKMSERGSIL 677

Query: 541 VRPDEHIAWRVKSGISGDPNTELIGVFTTLL 564
           VRPD+HIAWR KSGI+ DP   +  VFT +L
Sbjct: 678 VRPDQHIAWRAKSGITLDPTLHMRDVFTIIL 706