Cucsat.G378.T4 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G378.T4
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionHydroxyisourate hydrolase
Locationctg1: 8066986 .. 8071044 (-)
RNA-Seq ExpressionCucsat.G378.T4
SyntenyCucsat.G378.T4
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATAATATTTGGATAGTAAGAGTATTCAAATTGATGGATTATTCTTAAATCCTATTTATTTCTATCTAATGGTATGATAAATCATTAACTCCATGAGAGCTAATTTAATTATATAATACATAACAAATTTTAACGTTTTTTTAATTTGGTGTCTTCATGTTTATTAAATTAATTGCATTATTAGGAATCTAAACTTGATTAATATACAAACGCCGCCACCGCCAGCGCAGCTGCAGATTTCCATCTAACGAACACATCGGCTGTTGAGTGAGGCTTAGAGACTCCGACTGTAGAGAAGGAAGAACGAGTGATCGGTGGCAATAACGATGGCGTCAAATTATGATTTTGTAGAGGAAGAGTTTCAGGCATGCTGTGGAAGCTCCAAATTCGCCAAAGAAATGGTTTCTGCTTCCCCTTTTTTCTCCTTGGAACAAGCTATAGCTGCAGCTAGACACATCTGGTTCAATCAGGTCTCTATTTTTTTTTTTTCTTTTTCCTTTTTTGAGCTCTTTGTATCATCGTTTGTGATCGATGGAGACTGAAAATTTGATGTTTCTCTTTTAAAGGTCGATGTGAGTGGTTGGTTGGAAGCATTCTCTGCTCATCCACGGATTGGTGGTCAAGTCTCCAAATCATCCAATCAGACTAGTGCTCAGTTAAGACCCTGACCCTTTAAATGCTAGATTCTTAATTATCTATCTTTCTCGTAGAAATCGAGTTGGTTGAATCACTGTTATCAGTTTATCCACTTGTTGCTTACAGTTAATTGAACAAAATTTGGAATAAGATAGTATTGTCTTAGAATGCAATTGAATGCTGGAACGTGAAGCTTGACTTCAGGTTGCCTGTGAGAGAGATCTATCTATGGTTTTGATTTTAACAGATGCCAATAGCCAAGTACTGAATTTGTTTGGAAATTGGGGTTGTATGATTCTGAAGCTATTTAACTGCCCAATTTTGATGGTTCTCAATCCACTCTGAGAAAAGAGGGATTGGACCAATATGTAGCTGTCTGAAAATCAAATATTTTTAGCAGAAACAGGGAGATGTAAGGGTCTTAGTTAAGGATACTAGTTTTAGGAAGTGAGTTAGTGTATTTTCTTATAAAGAGGGAGGAGGATTGAGGGAAGGCATGCGATTGTTGTGTGATTTAGGGCTTGGGTTGGCATACTCAAGAGGGGAGGTTCCGATGTCTTATACTCGGTTTATCTTGTAGTTCCTCATCCTTTTTATCTTTCAATGTATCTTAGATTTAGGGTCTTGTTGGGTGATGTCCTAGCAGGAGACTTTAATTTCGTTTCTTCATTATAGGTTTTCGTTGTTCTTACATTTTTCCTTTCAACTGTTGATTAGATTGCTTGTATAAATATGTATGATATTGTACACGTGAAGTGACTTTTCAATGAAATTCTTCATAGATGGAGTAAGGGAGAACAGTCAACTGCAATGGCAACTGCCACTGGTTCTAGCTTACAGGTTATAGCATGCTGCCCTCTTACTGAGAAATACGAAAAAGTAGTTCGAGTCTGTTTTAGTATCATCCAATTGACTGCTTTTTTGGAAGACGGGTTTGGGTTTGAGTTATTGATGATTAATTGTCAATGATTTCTAATGTGTACTTTTGCATAATACTTGCTGGCCTTTGCAGGAGCTAGCAGAATGGAATATTCAGTATCAGCAGAAGTTTGGTTTTGTTTTTCTTATTTGTGCATCTGGAAGGAGCACTAGCGAGATTCTAGCAGAATTGAAGGTAGATCCTTCATCGAAATTCATGATGATGGTCCCTAGTATTTAAGGCTTTGTGGTTTTAATTTTTCTATTTTTGAAATTAATGCTTGTTTCCTTATGTATTATTGGCTAGTTTTTCACTTTACTTAAACACGTGGATTCTAAACAAAATTTCAAAAACAAATTTTCTTAGTCTTCAAATTTTGGTTTGGTTTCTGAAAACATGTTTAAAACGTGGGTGGTAGGTGAAAAAGACTATTGGTGGAAATATTGTGGTTAAGCATAGTTTTAAAAAAAAAAGTAAAATGGGTGTCATATAGGACCTTAGATGTTCGTTGTTTTAACCCTTTTTAGGATTGAATAGTTTCAAATAATACAATAGACTAATTTGTCTAAAGCGTATAGTGATCTCAGGAGCACCTGTTTATTAGGAAACTTCAACCAACATACCAATGAGGCGATTACATGAATTAGATAAAGGATTTCTATAATCTTTGTGTTGCTTCATCTTCTTGCTTAAAACACAGTCACTGAGTTCAATTAAATGGAATACCCTTTCATTCTTACTGGAAGACTGTATATTTGTATTGACACCCAGCTTAATAATGAGAAGCCATTGGCATTACTGTGGTGAATTGCAATAATATCAGTTACTTTTCCTAGTATTTGTTCATATTTTCGGGTCAGGGGTGCAATTCCTAAGATAATCTTGTCAGCTATCCTTAGAATTCAACTGAGGACTGGGTTTCTTAGTTGGAAGTTTGAGTTATGTTCCATTTATCTAAAACTCTAATTGACCCCTCTTCATTCGGTTGAAAATCTTTTCATCGTAGTATGAGAAAACTGTGCCTGTTGCAGAAACGGTATCCAAGTAGACCCATAGTTGAGTTTGAGATTGCTGCTCAGGAGCAAATGAAGATAACAGAACTGCGTCTTGCCAAACTTTTCTCAACCAAACAAAATACCTCTTCAACCAGAGAGAGTCATCCTCCTCCGGATTTTGCACGAAAAGTAGAAGGTAAACTTCATTTACAAATATAAAAAAAGAACTTGCAAAGAGTACTTTTATCTGTTTCATCTTTGTGTGTTCATAGCATCCTTGGAAATCCAATTCTCGTTTAATCTGCCAATTAAGAATGCATCAATTCAAAAAACAAAGAGATGAACCGATTAAAAAACTGTTTCTCAGGAGGGGGAAAAAAATTAATTCTTAAACCGGACACATTGTTTTAAGATGAAAACCATATAAGTGCTGCTATCAATAGTTACATATATTGAGCTTTCTTGATTTTTTGCATTTTAAGCAATAATTATTCATCTTAATTCTTTTAAATGTTTGGCGTATCGTTTCCGATTTGAGAGAATAATAGGATTTTTAGGTCCTATTAGAGAGGCTGAAAGAACCAGTGAGGATGTTCAGGAGGGGATCAGTTTAAAAGCAATCCTTTTAAATTATTTCCTTTACTATACCAATGTTAACTGTTAAGCTGGATGGATGAACATGACATGATCATTTCCAAGTATAATAGGTTGATTGAATTGATATGTTGTGGTAACACTCCTTCTCATTTGCTCATTTTGAAAATCTAGAAGATCGTGTGAACATTATTGGAGGGCATCTAGGTGCTACTTCTGAAGCTTCAACTGGAAAGACATCACAAATTTTAACTCGAACTCGACCCCCAATTACAACCCATGTGTTAGATGTTGCACGAGGATCTCCAGCTACAGGTATTGACGTCCGTTTGGAAAGATGGATCGGTACTCAACCATGCCCCTTATTCGGTGAGGCCGATGTTGGGGGCTGGGCACTCGAAGGGACATCAGAAACAGATAAAGATGGGAGAAGTGGGCAATTGTTGAGCATGGTTGAAGCTATAAATCCAGGAATTTACAGAATAAGTTTTAACACGGGAAAGTATTTCCCGGCTGGGTTCTTTCCGTACGTTTCCATTGTGTTCGAAATCAGGGAATCCCAGAAGTTAGAACACTTCCATGTCCCTCTCCTGCTTTCACCATTTTCATTCTCCACATATCGTGGAAGCTAGAAGTTTTCTGTGCATGTGACCGTTTCGGTGTTTCGGTGTTTCAGCAAAAGACGAATAAAGCAATGTCGATTGGTATCAAATGTTGTATAACATAAAAAAAACTCTTACGCAAATTATGTAATAAATATTTAGATATCTACATGAAGCTTGTACTCGGGCTTAAGTCTTTTTTATGTGTCTACAATGTATAATTAACTGGATAGTATTTTCAGCTTATGATTGAAAGATGGTCCTAAAGTGCAAAGTATTTGAATCTTGTTTGTAAATTTCATGGCCAAACAAAC

Coding sequence (CDS)

ATGGCGTCAAATTATGATTTTGTAGAGGAAGAGTTTCAGGCATGCTGTGGAAGCTCCAAATTCGCCAAAGAAATGGTTTCTGCTTCCCCTTTTTTCTCCTTGGAACAAGCTATAGCTGCAGCTAGACACATCTGGTTCAATCAGGTCGATGTGAGTGGTTGGTTGGAAGCATTCTCTGCTCATCCACGGATTGGTGGTCAAGTCTCCAAATCATCCAATCAGACTAGTGCTCAATGGAGTAAGGGAGAACAGTCAACTGCAATGGCAACTGCCACTGGTTCTAGCTTACAGGAGCTAGCAGAATGGAATATTCAGTATCAGCAGAAGTTTGGTTTTGTTTTTCTTATTTGTGCATCTGGAAGGAGCACTAGCGAGATTCTAGCAGAATTGAAGAAACGGTATCCAAGTAGACCCATAGTTGAGTTTGAGATTGCTGCTCAGGAGCAAATGAAGATAACAGAACTGCGTCTTGCCAAACTTTTCTCAACCAAACAAAATACCTCTTCAACCAGAGAGAGTCATCCTCCTCCGGATTTTGCACGAAAAGTAGAAGAAGATCGTGTGAACATTATTGGAGGGCATCTAGGTGCTACTTCTGAAGCTTCAACTGGAAAGACATCACAAATTTTAACTCGAACTCGACCCCCAATTACAACCCATGTGTTAGATGTTGCACGAGGATCTCCAGCTACAGGTATTGACGTCCGTTTGGAAAGATGGATCGGTACTCAACCATGCCCCTTATTCGGTGAGGCCGATGTTGGGGGCTGGGCACTCGAAGGGACATCAGAAACAGATAAAGATGGGAGAAGTGGGCAATTGTTGAGCATGGTTGAAGCTATAAATCCAGGAATTTACAGAATAAGTTTTAACACGGGAAAGTATTTCCCGGCTGGGTTCTTTCCGTACGTTTCCATTGTGTTCGAAATCAGGGAATCCCAGAAGTTAGAACACTTCCATGTCCCTCTCCTGCTTTCACCATTTTCATTCTCCACATATCGTGGAAGCTAG

Protein sequence

MASNYDFVEEEFQACCGSSKFAKEMVSASPFFSLEQAIAAARHIWFNQVDVSGWLEAFSAHPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASGRSTSEILAELKKRYPSRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFARKVEEDRVNIIGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPATGIDVRLERWIGTQPCPLFGEADVGGWALEGTSETDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGFFPYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS
Homology
BLAST of Cucsat.G378.T4 vs. ExPASy Swiss-Prot
Match: Q9LVM5 (Uric acid degradation bifunctional protein TTL OS=Arabidopsis thaliana OX=3702 GN=TTL PE=1 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 7.9e-103
Identity = 196/328 (59.76%), Postives = 242/328 (73.78%), Query Frame = 0

Query: 9   EEEFQACCGSSKFAKEMVSASPFFSLEQAIAAARHIWFNQVDVSGWLEAFSAHPRIGGQV 68
           E+E++ CCGSS+FAK+M ++ P  S ++AI  AR IWFNQV+V+ WLEAFSAHP+IG   
Sbjct: 7   EDEWKVCCGSSEFAKQMSTSGPLTS-QEAIYTARDIWFNQVNVTDWLEAFSAHPQIGNTP 66

Query: 69  SKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASGRSTSEILA 128
           S S N   A+ S  EQSTA AT + S+LQELAEWN+ Y++KFGF+F+ICASGR+ +E+L 
Sbjct: 67  SPSINSDFARRSVSEQSTAFATTSASALQELAEWNVLYKKKFGFIFIICASGRTHAEMLH 126

Query: 129 ELKKRYPSRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFARKVEEDRV 188
            LK+RY +RPIVE EIAA EQMKITELR+AKLFS K    S  +S   P   +   +DR+
Sbjct: 127 ALKERYENRPIVELEIAAMEQMKITELRMAKLFSDKAKVISETDSSSSPVSTK--PQDRL 186

Query: 189 NIIGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPATGIDVRLERWIGTQPCPL 248
            IIGGHL   +EA   K      R+RPPITTHVLDV+RG+PA G++V LE W GT   P 
Sbjct: 187 RIIGGHLNVAAEAKAPK------RSRPPITTHVLDVSRGAPAAGVEVHLEVWSGTTG-PS 246

Query: 249 FGEADVGGWALEGTSETDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGFFPYVSIVF 308
           F     G W+  GTS TD+DGRSG L+ +V+A+NPG YRISF+T KY P  FFPYVSIVF
Sbjct: 247 FVHGGGGVWSSVGTSATDRDGRSGPLMDLVDALNPGTYRISFDTAKYSPGCFFPYVSIVF 306

Query: 309 EIRESQKLEHFHVPLLLSPFSFSTYRGS 337
           ++ ESQK EHFHVPLLL+PFSFSTYRGS
Sbjct: 307 QVTESQKWEHFHVPLLLAPFSFSTYRGS 324

BLAST of Cucsat.G378.T4 vs. ExPASy Swiss-Prot
Match: Q9CRB3 (5-hydroxyisourate hydrolase OS=Mus musculus OX=10090 GN=Urah PE=1 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 7.8e-18
Identity = 58/126 (46.03%), Postives = 76/126 (60.32%), Query Frame = 0

Query: 216 PITTHVLDVARGSPATGIDVRLERWIGTQPCPLFGEADVGGWALEGTSETDKDGRSGQLL 275
           P+TTHVLD A G PA G+ +RL R           EA    W    TS T+ DGR   LL
Sbjct: 7   PLTTHVLDTASGLPAQGLCLRLSRL----------EAPCQQWMELRTSYTNLDGRCPGLL 66

Query: 276 SMVEAINPGIYRISFNTGKYF----PAGFFPYVSIVFEI-RESQKLEHFHVPLLLSPFSF 335
           +  + I PG Y++ F+T +Y+       F+PYV +VF I +E+QK   FHVPLLLSP+S+
Sbjct: 67  TPSQ-IKPGTYKLFFDTERYWKERGQESFYPYVEVVFTITKETQK---FHVPLLLSPWSY 118

Query: 336 STYRGS 337
           +TYRGS
Sbjct: 127 TTYRGS 118

BLAST of Cucsat.G378.T4 vs. ExPASy Swiss-Prot
Match: Q06S87 (5-hydroxyisourate hydrolase OS=Danio rerio OX=7955 GN=urah PE=1 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 7.3e-16
Identity = 56/155 (36.13%), Postives = 81/155 (52.26%), Query Frame = 0

Query: 186 DRVNIIGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPATGIDVRLERWIGTQP 245
           +R+  I GH+      S  K   +      P++THVL++A+G P   + + L R      
Sbjct: 2   NRLQHIRGHI-----VSADKHINMSATLLSPLSTHVLNIAQGVPGANMTIVLHRL----- 61

Query: 246 CPLFGEADVGGWALEGTSETDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPA----GFF 305
                +     W +  T  T+ DGR   L++  E    G+Y++ F TGKY+ A     F+
Sbjct: 62  -----DPVSSAWNILTTGITNDDGRCPGLITK-ENFIAGVYKMRFETGKYWDALGETCFY 121

Query: 306 PYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS 337
           PYV IVF I  +   +H+HVPLLLS FS+STYRGS
Sbjct: 122 PYVEIVFTITNTS--QHYHVPLLLSRFSYSTYRGS 138

BLAST of Cucsat.G378.T4 vs. ExPASy Swiss-Prot
Match: Q4VYA5 (5-hydroxyisourate hydrolase OS=Salmonella dublin OX=98360 GN=hiuH PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 2.1e-15
Identity = 50/124 (40.32%), Postives = 68/124 (54.84%), Query Frame = 0

Query: 217 ITTHVLDVARGSPATGIDVRLERWIGTQPCPLFGEADVGGWALEGTSETDKDGRSGQLLS 276
           ++ H+LD   G PA G++V LE            +    GW    T  TD+DGR  + L 
Sbjct: 28  LSVHILDQQTGKPAPGVEVVLE------------QKKDNGWTQLNTGHTDQDGRI-KALW 87

Query: 277 MVEAINPGIYRISFNTGKYFPA----GFFPYVSIVFEIRESQKLEHFHVPLLLSPFSFST 336
             +A  PG YR+ F TG+YF +     FFP + + F I  S+  EH+HVPLLLS + +ST
Sbjct: 88  PEKAAAPGDYRVIFKTGQYFESKKLDTFFPEIPVEFHI--SKTNEHYHVPLLLSQYGYST 136

BLAST of Cucsat.G378.T4 vs. ExPASy Swiss-Prot
Match: Q8Z7Q6 (5-hydroxyisourate hydrolase OS=Salmonella typhi OX=90370 GN=hiuH PE=3 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 2.1e-15
Identity = 50/124 (40.32%), Postives = 68/124 (54.84%), Query Frame = 0

Query: 217 ITTHVLDVARGSPATGIDVRLERWIGTQPCPLFGEADVGGWALEGTSETDKDGRSGQLLS 276
           ++ H+LD   G PA G++V LE            +    GW    T  TD+DGR  + L 
Sbjct: 28  LSVHILDQQTGKPAPGVEVVLE------------QKKDNGWTQLNTGHTDQDGRI-KALW 87

Query: 277 MVEAINPGIYRISFNTGKYFPA----GFFPYVSIVFEIRESQKLEHFHVPLLLSPFSFST 336
             +A  PG YR+ F TG+YF +     FFP + + F I  S+  EH+HVPLLLS + +ST
Sbjct: 88  PEKAAAPGDYRVIFKTGQYFESKKLDTFFPEIPVEFHI--SKTNEHYHVPLLLSQYGYST 136

BLAST of Cucsat.G378.T4 vs. NCBI nr
Match: XP_004133944.1 (uric acid degradation bifunctional protein TTL isoform X1 [Cucumis sativus] >KGN56645.1 hypothetical protein Csa_010326 [Cucumis sativus])

HSP 1 Score: 660 bits (1704), Expect = 4.16e-239
Identity = 333/336 (99.11%), Postives = 334/336 (99.40%), Query Frame = 0

Query: 1   MASNYDFVEEEFQACCGSSKFAKEMVSASPFFSLEQAIAAARHIWFNQVDVSGWLEAFSA 60
           MASNYDFVEEEFQACCGSSKFAKEMVSASPFFSLEQAIAAARHIWFNQVDVSGWLEAFSA
Sbjct: 1   MASNYDFVEEEFQACCGSSKFAKEMVSASPFFSLEQAIAAARHIWFNQVDVSGWLEAFSA 60

Query: 61  HPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASG 120
           HPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASG
Sbjct: 61  HPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASG 120

Query: 121 RSTSEILAELKKRYPSRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFA 180
           RSTSEILAELKKRYPSRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFA
Sbjct: 121 RSTSEILAELKKRYPSRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFA 180

Query: 181 RKVEEDRVNIIGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPATGIDVRLERW 240
           RKVEEDRVN+IGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPATGIDVRLERW
Sbjct: 181 RKVEEDRVNVIGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPATGIDVRLERW 240

Query: 241 IGTQPCPLFGEADVGGWALEGTSETDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGF 300
           IGTQP PLFGEADVGGWALEGTS TDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGF
Sbjct: 241 IGTQPRPLFGEADVGGWALEGTSATDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGF 300

Query: 301 FPYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS 336
           FPYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS
Sbjct: 301 FPYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS 336

BLAST of Cucsat.G378.T4 vs. NCBI nr
Match: XP_011650818.1 (uric acid degradation bifunctional protein TTL isoform X2 [Cucumis sativus])

HSP 1 Score: 654 bits (1687), Expect = 1.56e-236
Identity = 332/336 (98.81%), Postives = 333/336 (99.11%), Query Frame = 0

Query: 1   MASNYDFVEEEFQACCGSSKFAKEMVSASPFFSLEQAIAAARHIWFNQVDVSGWLEAFSA 60
           MASNYDFVEEEFQACCGSSKFAKEMVSASPFFSLEQAIAAARHIWFNQVDVSGWLEAFSA
Sbjct: 1   MASNYDFVEEEFQACCGSSKFAKEMVSASPFFSLEQAIAAARHIWFNQVDVSGWLEAFSA 60

Query: 61  HPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASG 120
           HPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASG
Sbjct: 61  HPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASG 120

Query: 121 RSTSEILAELKKRYPSRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFA 180
           RSTSEILAELKKRYPSRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFA
Sbjct: 121 RSTSEILAELKKRYPSRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFA 180

Query: 181 RKVEEDRVNIIGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPATGIDVRLERW 240
           RKVE DRVN+IGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPATGIDVRLERW
Sbjct: 181 RKVE-DRVNVIGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPATGIDVRLERW 240

Query: 241 IGTQPCPLFGEADVGGWALEGTSETDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGF 300
           IGTQP PLFGEADVGGWALEGTS TDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGF
Sbjct: 241 IGTQPRPLFGEADVGGWALEGTSATDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGF 300

Query: 301 FPYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS 336
           FPYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS
Sbjct: 301 FPYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS 335

BLAST of Cucsat.G378.T4 vs. NCBI nr
Match: XP_008438229.1 (PREDICTED: uric acid degradation bifunctional protein TTL isoform X1 [Cucumis melo])

HSP 1 Score: 647 bits (1669), Expect = 8.99e-234
Identity = 327/336 (97.32%), Postives = 329/336 (97.92%), Query Frame = 0

Query: 1   MASNYDFVEEEFQACCGSSKFAKEMVSASPFFSLEQAIAAARHIWFNQVDVSGWLEAFSA 60
           MASNYDF EEEFQACCGSSKFAKEMVSASPF SLEQAIAAARHIWFNQVDVSGWLEAFSA
Sbjct: 1   MASNYDFGEEEFQACCGSSKFAKEMVSASPFSSLEQAIAAARHIWFNQVDVSGWLEAFSA 60

Query: 61  HPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASG 120
           HPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASG
Sbjct: 61  HPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASG 120

Query: 121 RSTSEILAELKKRYPSRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFA 180
           RSTSEILAELKKRYP+RPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFA
Sbjct: 121 RSTSEILAELKKRYPNRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFA 180

Query: 181 RKVEEDRVNIIGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPATGIDVRLERW 240
           RKVEEDRVN+IGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPA GIDV LERW
Sbjct: 181 RKVEEDRVNVIGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPAAGIDVCLERW 240

Query: 241 IGTQPCPLFGEADVGGWALEGTSETDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGF 300
           IGTQP PLFGE DVGGWALEGTS TDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGF
Sbjct: 241 IGTQPRPLFGEGDVGGWALEGTSATDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGF 300

Query: 301 FPYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS 336
           FPYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS
Sbjct: 301 FPYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS 336

BLAST of Cucsat.G378.T4 vs. NCBI nr
Match: XP_008438230.1 (PREDICTED: uric acid degradation bifunctional protein TTL isoform X2 [Cucumis melo])

HSP 1 Score: 640 bits (1652), Expect = 3.38e-231
Identity = 326/336 (97.02%), Postives = 328/336 (97.62%), Query Frame = 0

Query: 1   MASNYDFVEEEFQACCGSSKFAKEMVSASPFFSLEQAIAAARHIWFNQVDVSGWLEAFSA 60
           MASNYDF EEEFQACCGSSKFAKEMVSASPF SLEQAIAAARHIWFNQVDVSGWLEAFSA
Sbjct: 1   MASNYDFGEEEFQACCGSSKFAKEMVSASPFSSLEQAIAAARHIWFNQVDVSGWLEAFSA 60

Query: 61  HPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASG 120
           HPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASG
Sbjct: 61  HPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASG 120

Query: 121 RSTSEILAELKKRYPSRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFA 180
           RSTSEILAELKKRYP+RPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFA
Sbjct: 121 RSTSEILAELKKRYPNRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFA 180

Query: 181 RKVEEDRVNIIGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPATGIDVRLERW 240
           RKVE DRVN+IGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPA GIDV LERW
Sbjct: 181 RKVE-DRVNVIGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPAAGIDVCLERW 240

Query: 241 IGTQPCPLFGEADVGGWALEGTSETDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGF 300
           IGTQP PLFGE DVGGWALEGTS TDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGF
Sbjct: 241 IGTQPRPLFGEGDVGGWALEGTSATDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGF 300

Query: 301 FPYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS 336
           FPYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS
Sbjct: 301 FPYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS 335

BLAST of Cucsat.G378.T4 vs. NCBI nr
Match: XP_038878038.1 (uric acid degradation bifunctional protein TTL isoform X1 [Benincasa hispida])

HSP 1 Score: 629 bits (1621), Expect = 1.86e-226
Identity = 316/336 (94.05%), Postives = 325/336 (96.73%), Query Frame = 0

Query: 1   MASNYDFVEEEFQACCGSSKFAKEMVSASPFFSLEQAIAAARHIWFNQVDVSGWLEAFSA 60
           MASNYDF EEEFQACCGSSKFAKEMVSASPF SLEQA+AAARHIWFNQVDV+GWLEAFSA
Sbjct: 1   MASNYDFREEEFQACCGSSKFAKEMVSASPFSSLEQAVAAARHIWFNQVDVNGWLEAFSA 60

Query: 61  HPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASG 120
           HPRIGGQVSKSS+QTSAQWSKGEQSTAMATATGSSLQELAEWN QYQQKFGFVFLICASG
Sbjct: 61  HPRIGGQVSKSSSQTSAQWSKGEQSTAMATATGSSLQELAEWNSQYQQKFGFVFLICASG 120

Query: 121 RSTSEILAELKKRYPSRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFA 180
           RSTSEILAELKKRY +RPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRES+ PPDF 
Sbjct: 121 RSTSEILAELKKRYSNRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESYSPPDFV 180

Query: 181 RKVEEDRVNIIGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPATGIDVRLERW 240
           RKVEEDRV+IIGGHLGATSEASTGKTSQ+LTRTRPPITTHVLDVARGSPA GIDVRLERW
Sbjct: 181 RKVEEDRVHIIGGHLGATSEASTGKTSQVLTRTRPPITTHVLDVARGSPAAGIDVRLERW 240

Query: 241 IGTQPCPLFGEADVGGWALEGTSETDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGF 300
           IGTQP PLFGE DVGGW LEG+S TDKDGRSGQLLSMVEA+NPGIYRISFNTGKYFPAGF
Sbjct: 241 IGTQPHPLFGEGDVGGWVLEGSSATDKDGRSGQLLSMVEALNPGIYRISFNTGKYFPAGF 300

Query: 301 FPYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS 336
           FPYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS
Sbjct: 301 FPYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS 336

BLAST of Cucsat.G378.T4 vs. ExPASy TrEMBL
Match: A0A0A0L3W8 (Hydroxyisourate hydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G127080 PE=4 SV=1)

HSP 1 Score: 660 bits (1704), Expect = 2.01e-239
Identity = 333/336 (99.11%), Postives = 334/336 (99.40%), Query Frame = 0

Query: 1   MASNYDFVEEEFQACCGSSKFAKEMVSASPFFSLEQAIAAARHIWFNQVDVSGWLEAFSA 60
           MASNYDFVEEEFQACCGSSKFAKEMVSASPFFSLEQAIAAARHIWFNQVDVSGWLEAFSA
Sbjct: 1   MASNYDFVEEEFQACCGSSKFAKEMVSASPFFSLEQAIAAARHIWFNQVDVSGWLEAFSA 60

Query: 61  HPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASG 120
           HPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASG
Sbjct: 61  HPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASG 120

Query: 121 RSTSEILAELKKRYPSRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFA 180
           RSTSEILAELKKRYPSRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFA
Sbjct: 121 RSTSEILAELKKRYPSRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFA 180

Query: 181 RKVEEDRVNIIGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPATGIDVRLERW 240
           RKVEEDRVN+IGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPATGIDVRLERW
Sbjct: 181 RKVEEDRVNVIGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPATGIDVRLERW 240

Query: 241 IGTQPCPLFGEADVGGWALEGTSETDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGF 300
           IGTQP PLFGEADVGGWALEGTS TDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGF
Sbjct: 241 IGTQPRPLFGEADVGGWALEGTSATDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGF 300

Query: 301 FPYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS 336
           FPYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS
Sbjct: 301 FPYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS 336

BLAST of Cucsat.G378.T4 vs. ExPASy TrEMBL
Match: A0A1S3AWH8 (Hydroxyisourate hydrolase OS=Cucumis melo OX=3656 GN=LOC103483398 PE=4 SV=1)

HSP 1 Score: 647 bits (1669), Expect = 4.35e-234
Identity = 327/336 (97.32%), Postives = 329/336 (97.92%), Query Frame = 0

Query: 1   MASNYDFVEEEFQACCGSSKFAKEMVSASPFFSLEQAIAAARHIWFNQVDVSGWLEAFSA 60
           MASNYDF EEEFQACCGSSKFAKEMVSASPF SLEQAIAAARHIWFNQVDVSGWLEAFSA
Sbjct: 1   MASNYDFGEEEFQACCGSSKFAKEMVSASPFSSLEQAIAAARHIWFNQVDVSGWLEAFSA 60

Query: 61  HPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASG 120
           HPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASG
Sbjct: 61  HPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASG 120

Query: 121 RSTSEILAELKKRYPSRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFA 180
           RSTSEILAELKKRYP+RPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFA
Sbjct: 121 RSTSEILAELKKRYPNRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFA 180

Query: 181 RKVEEDRVNIIGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPATGIDVRLERW 240
           RKVEEDRVN+IGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPA GIDV LERW
Sbjct: 181 RKVEEDRVNVIGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPAAGIDVCLERW 240

Query: 241 IGTQPCPLFGEADVGGWALEGTSETDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGF 300
           IGTQP PLFGE DVGGWALEGTS TDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGF
Sbjct: 241 IGTQPRPLFGEGDVGGWALEGTSATDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGF 300

Query: 301 FPYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS 336
           FPYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS
Sbjct: 301 FPYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS 336

BLAST of Cucsat.G378.T4 vs. ExPASy TrEMBL
Match: A0A1S3AVJ3 (Hydroxyisourate hydrolase OS=Cucumis melo OX=3656 GN=LOC103483398 PE=4 SV=1)

HSP 1 Score: 640 bits (1652), Expect = 1.64e-231
Identity = 326/336 (97.02%), Postives = 328/336 (97.62%), Query Frame = 0

Query: 1   MASNYDFVEEEFQACCGSSKFAKEMVSASPFFSLEQAIAAARHIWFNQVDVSGWLEAFSA 60
           MASNYDF EEEFQACCGSSKFAKEMVSASPF SLEQAIAAARHIWFNQVDVSGWLEAFSA
Sbjct: 1   MASNYDFGEEEFQACCGSSKFAKEMVSASPFSSLEQAIAAARHIWFNQVDVSGWLEAFSA 60

Query: 61  HPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASG 120
           HPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASG
Sbjct: 61  HPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASG 120

Query: 121 RSTSEILAELKKRYPSRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFA 180
           RSTSEILAELKKRYP+RPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFA
Sbjct: 121 RSTSEILAELKKRYPNRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFA 180

Query: 181 RKVEEDRVNIIGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPATGIDVRLERW 240
           RKVE DRVN+IGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPA GIDV LERW
Sbjct: 181 RKVE-DRVNVIGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPAAGIDVCLERW 240

Query: 241 IGTQPCPLFGEADVGGWALEGTSETDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGF 300
           IGTQP PLFGE DVGGWALEGTS TDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGF
Sbjct: 241 IGTQPRPLFGEGDVGGWALEGTSATDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGF 300

Query: 301 FPYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS 336
           FPYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS
Sbjct: 301 FPYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS 335

BLAST of Cucsat.G378.T4 vs. ExPASy TrEMBL
Match: A0A5D3D0I5 (Uric acid degradation bifunctional protein TTL isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003520 PE=4 SV=1)

HSP 1 Score: 604 bits (1558), Expect = 6.74e-217
Identity = 306/315 (97.14%), Postives = 308/315 (97.78%), Query Frame = 0

Query: 1   MASNYDFVEEEFQACCGSSKFAKEMVSASPFFSLEQAIAAARHIWFNQVDVSGWLEAFSA 60
           MASNYDF EEEFQACCGSSKFAKEMVSASPF SLEQAIAAARHIWFNQVDVSGWLEAFSA
Sbjct: 1   MASNYDFGEEEFQACCGSSKFAKEMVSASPFSSLEQAIAAARHIWFNQVDVSGWLEAFSA 60

Query: 61  HPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASG 120
           HPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASG
Sbjct: 61  HPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASG 120

Query: 121 RSTSEILAELKKRYPSRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFA 180
           RSTSEILAELKKRYP+RPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFA
Sbjct: 121 RSTSEILAELKKRYPNRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFA 180

Query: 181 RKVEEDRVNIIGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPATGIDVRLERW 240
           RKVEEDRVN+IGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPA GIDV LERW
Sbjct: 181 RKVEEDRVNVIGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPAAGIDVCLERW 240

Query: 241 IGTQPCPLFGEADVGGWALEGTSETDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGF 300
           IGTQP PLFGE DVGGWALEGTS TDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGF
Sbjct: 241 IGTQPRPLFGEGDVGGWALEGTSATDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGF 300

Query: 301 FPYVSIVFEIRESQK 315
           FPYVSIVFEIRESQK
Sbjct: 301 FPYVSIVFEIRESQK 315

BLAST of Cucsat.G378.T4 vs. ExPASy TrEMBL
Match: A0A6J1G375 (Hydroxyisourate hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111450364 PE=4 SV=1)

HSP 1 Score: 592 bits (1525), Expect = 3.56e-212
Identity = 300/336 (89.29%), Postives = 315/336 (93.75%), Query Frame = 0

Query: 1   MASNYDFVEEEFQACCGSSKFAKEMVSASPFFSLEQAIAAARHIWFNQVDVSGWLEAFSA 60
           MA++YDF EEEFQACCGSSKFA+EMVS SPFFSLEQA+ AAR IWFNQVDV+GWLEAFSA
Sbjct: 1   MAASYDFGEEEFQACCGSSKFAREMVSVSPFFSLEQAVDAARRIWFNQVDVNGWLEAFSA 60

Query: 61  HPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASG 120
           HP+IG Q SKSSNQTSAQWSKGEQSTA+ATATGSSLQELAEWNIQYQ+KFGF+FLICASG
Sbjct: 61  HPQIG-QASKSSNQTSAQWSKGEQSTALATATGSSLQELAEWNIQYQKKFGFIFLICASG 120

Query: 121 RSTSEILAELKKRYPSRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFA 180
           RST EILAELKKRY +RPIVEFEIAAQEQMKITELRLAKLFSTKQN SST+ES+PP  FA
Sbjct: 121 RSTDEILAELKKRYSNRPIVEFEIAAQEQMKITELRLAKLFSTKQNASSTKESYPPA-FA 180

Query: 181 RKVEEDRVNIIGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPATGIDVRLERW 240
           RKVEEDRV+IIGGHL   S ASTGKT Q LTRTRPPITTHVLDVARGSPA GIDVRLERW
Sbjct: 181 RKVEEDRVSIIGGHLSTASGASTGKTLQTLTRTRPPITTHVLDVARGSPAAGIDVRLERW 240

Query: 241 IGTQPCPLFGEADVGGWALEGTSETDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGF 300
           IGTQP PLFGE DVGGWALEG+S TDKDGRSGQLLSM+EAINPGIYRISFNTGKYFPAGF
Sbjct: 241 IGTQPRPLFGEVDVGGWALEGSSATDKDGRSGQLLSMIEAINPGIYRISFNTGKYFPAGF 300

Query: 301 FPYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS 336
           FPYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS
Sbjct: 301 FPYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS 334

BLAST of Cucsat.G378.T4 vs. TAIR 10
Match: AT5G58220.1 (transthyretin-like protein )

HSP 1 Score: 375.2 bits (962), Expect = 5.6e-104
Identity = 196/328 (59.76%), Postives = 242/328 (73.78%), Query Frame = 0

Query: 9   EEEFQACCGSSKFAKEMVSASPFFSLEQAIAAARHIWFNQVDVSGWLEAFSAHPRIGGQV 68
           E+E++ CCGSS+FAK+M ++ P  S ++AI  AR IWFNQV+V+ WLEAFSAHP+IG   
Sbjct: 7   EDEWKVCCGSSEFAKQMSTSGPLTS-QEAIYTARDIWFNQVNVTDWLEAFSAHPQIGNTP 66

Query: 69  SKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASGRSTSEILA 128
           S S N   A+ S  EQSTA AT + S+LQELAEWN+ Y++KFGF+F+ICASGR+ +E+L 
Sbjct: 67  SPSINSDFARRSVSEQSTAFATTSASALQELAEWNVLYKKKFGFIFIICASGRTHAEMLH 126

Query: 129 ELKKRYPSRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFARKVEEDRV 188
            LK+RY +RPIVE EIAA EQMKITELR+AKLFS K    S  +S   P   +   +DR+
Sbjct: 127 ALKERYENRPIVELEIAAMEQMKITELRMAKLFSDKAKVISETDSSSSPVSTK--PQDRL 186

Query: 189 NIIGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPATGIDVRLERWIGTQPCPL 248
            IIGGHL   +EA   K      R+RPPITTHVLDV+RG+PA G++V LE W GT   P 
Sbjct: 187 RIIGGHLNVAAEAKAPK------RSRPPITTHVLDVSRGAPAAGVEVHLEVWSGTTG-PS 246

Query: 249 FGEADVGGWALEGTSETDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGFFPYVSIVF 308
           F     G W+  GTS TD+DGRSG L+ +V+A+NPG YRISF+T KY P  FFPYVSIVF
Sbjct: 247 FVHGGGGVWSSVGTSATDRDGRSGPLMDLVDALNPGTYRISFDTAKYSPGCFFPYVSIVF 306

Query: 309 EIRESQKLEHFHVPLLLSPFSFSTYRGS 337
           ++ ESQK EHFHVPLLL+PFSFSTYRGS
Sbjct: 307 QVTESQKWEHFHVPLLLAPFSFSTYRGS 324

BLAST of Cucsat.G378.T4 vs. TAIR 10
Match: AT5G58220.3 (transthyretin-like protein )

HSP 1 Score: 351.7 bits (901), Expect = 6.7e-97
Identity = 188/328 (57.32%), Postives = 232/328 (70.73%), Query Frame = 0

Query: 9   EEEFQACCGSSKFAKEMVSASPFFSLEQAIAAARHIWFNQVDVSGWLEAFSAHPRIGGQV 68
           E+E++ CCGSS+FAK+M ++ P  S ++AI  AR IWFNQV+V+ WLEAFSAHP+IG   
Sbjct: 7   EDEWKVCCGSSEFAKQMSTSGPLTS-QEAIYTARDIWFNQVNVTDWLEAFSAHPQIGNTP 66

Query: 69  SKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASGRSTSEILA 128
           S S N   A+ S  EQSTA AT + S+LQELAEWN+ Y++KFGF+F+ICASGR+ +E+L 
Sbjct: 67  SPSINSDFARRSVSEQSTAFATTSASALQELAEWNVLYKKKFGFIFIICASGRTHAEMLH 126

Query: 129 ELKKRYPSRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFARKVEEDRV 188
            LK+RY +RPIVE EIAA EQMKITELR+AKLFS K    S  +S   P   +       
Sbjct: 127 ALKERYENRPIVELEIAAMEQMKITELRMAKLFSDKAKVISETDSSSSPVSTK------- 186

Query: 189 NIIGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPATGIDVRLERWIGTQPCPL 248
                     +EA   K      R+RPPITTHVLDV+RG+PA G++V LE W GT   P 
Sbjct: 187 --------PQAEAKAPK------RSRPPITTHVLDVSRGAPAAGVEVHLEVWSGTTG-PS 246

Query: 249 FGEADVGGWALEGTSETDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGFFPYVSIVF 308
           F     G W+  GTS TD+DGRSG L+ +V+A+NPG YRISF+T KY P  FFPYVSIVF
Sbjct: 247 FVHGGGGVWSSVGTSATDRDGRSGPLMDLVDALNPGTYRISFDTAKYSPGCFFPYVSIVF 306

Query: 309 EIRESQKLEHFHVPLLLSPFSFSTYRGS 337
           ++ ESQK EHFHVPLLL+PFSFSTYRGS
Sbjct: 307 QVTESQKWEHFHVPLLLAPFSFSTYRGS 311

BLAST of Cucsat.G378.T4 vs. TAIR 10
Match: AT5G58220.2 (transthyretin-like protein )

HSP 1 Score: 310.8 bits (795), Expect = 1.3e-84
Identity = 172/328 (52.44%), Postives = 212/328 (64.63%), Query Frame = 0

Query: 9   EEEFQACCGSSKFAKEMVSASPFFSLEQAIAAARHIWFNQVDVSGWLEAFSAHPRIGGQV 68
           E+E++ CCGSS+FAK+M ++ P  S ++AI  AR IWFNQV+V+ WLEAFSAHP+IG   
Sbjct: 7   EDEWKVCCGSSEFAKQMSTSGPLTS-QEAIYTARDIWFNQVNVTDWLEAFSAHPQIGNTP 66

Query: 69  SKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASGRSTSEILA 128
           S S N   A+ S  EQSTA AT + S+LQELAEWN+ Y++KFGF+F+ICASGR+ +E+L 
Sbjct: 67  SPSINSDFARRSVSEQSTAFATTSASALQELAEWNVLYKKKFGFIFIICASGRTHAEMLH 126

Query: 129 ELKKRYPSRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFARKVEEDRV 188
            LK+RY +RPIVE EIAA EQMKITELR+AKLFS K    S  +S   P           
Sbjct: 127 ALKERYENRPIVELEIAAMEQMKITELRMAKLFSDKAKVISETDSSSSP----------- 186

Query: 189 NIIGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPATGIDVRLERWIGTQPCPL 248
                                   T+P              A G++V LE W GT   P 
Sbjct: 187 ----------------------VSTKP-------------QAAGVEVHLEVWSGTTG-PS 246

Query: 249 FGEADVGGWALEGTSETDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGFFPYVSIVF 308
           F     G W+  GTS TD+DGRSG L+ +V+A+NPG YRISF+T KY P  FFPYVSIVF
Sbjct: 247 FVHGGGGVWSSVGTSATDRDGRSGPLMDLVDALNPGTYRISFDTAKYSPGCFFPYVSIVF 286

Query: 309 EIRESQKLEHFHVPLLLSPFSFSTYRGS 337
           ++ ESQK EHFHVPLLL+PFSFSTYRGS
Sbjct: 307 QVTESQKWEHFHVPLLLAPFSFSTYRGS 286

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LVM57.9e-10359.76Uric acid degradation bifunctional protein TTL OS=Arabidopsis thaliana OX=3702 G... [more]
Q9CRB37.8e-1846.035-hydroxyisourate hydrolase OS=Mus musculus OX=10090 GN=Urah PE=1 SV=1[more]
Q06S877.3e-1636.135-hydroxyisourate hydrolase OS=Danio rerio OX=7955 GN=urah PE=1 SV=1[more]
Q4VYA52.1e-1540.325-hydroxyisourate hydrolase OS=Salmonella dublin OX=98360 GN=hiuH PE=1 SV=1[more]
Q8Z7Q62.1e-1540.325-hydroxyisourate hydrolase OS=Salmonella typhi OX=90370 GN=hiuH PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004133944.14.16e-23999.11uric acid degradation bifunctional protein TTL isoform X1 [Cucumis sativus] >KGN... [more]
XP_011650818.11.56e-23698.81uric acid degradation bifunctional protein TTL isoform X2 [Cucumis sativus][more]
XP_008438229.18.99e-23497.32PREDICTED: uric acid degradation bifunctional protein TTL isoform X1 [Cucumis me... [more]
XP_008438230.13.38e-23197.02PREDICTED: uric acid degradation bifunctional protein TTL isoform X2 [Cucumis me... [more]
XP_038878038.11.86e-22694.05uric acid degradation bifunctional protein TTL isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A0A0L3W82.01e-23999.11Hydroxyisourate hydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G127080 PE=4 SV=1[more]
A0A1S3AWH84.35e-23497.32Hydroxyisourate hydrolase OS=Cucumis melo OX=3656 GN=LOC103483398 PE=4 SV=1[more]
A0A1S3AVJ31.64e-23197.02Hydroxyisourate hydrolase OS=Cucumis melo OX=3656 GN=LOC103483398 PE=4 SV=1[more]
A0A5D3D0I56.74e-21797.14Uric acid degradation bifunctional protein TTL isoform X1 OS=Cucumis melo var. m... [more]
A0A6J1G3753.56e-21289.29Hydroxyisourate hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111450364 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
AT5G58220.15.6e-10459.76transthyretin-like protein [more]
AT5G58220.36.7e-9757.32transthyretin-like protein [more]
AT5G58220.21.3e-8452.44transthyretin-like protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023416Transthyretin/hydroxyisourate hydrolase domainPFAMPF00576Transthyretincoord: 217..335
e-value: 2.5E-32
score: 111.8
IPR023416Transthyretin/hydroxyisourate hydrolase domainPANTHERPTHR10395URICASE AND TRANSTHYRETIN-RELATEDcoord: 6..336
IPR036817Transthyretin/hydroxyisourate hydrolase domain superfamilyGENE3D2.60.40.180Transthyretin/hydroxyisourate hydrolase domaincoord: 206..334
e-value: 6.0E-39
score: 134.7
IPR036817Transthyretin/hydroxyisourate hydrolase domain superfamilySUPERFAMILY49472Transthyretin (synonym: prealbumin)coord: 213..336
IPR014306Hydroxyisourate hydrolaseTIGRFAMTIGR02962TIGR02962coord: 216..336
e-value: 7.3E-29
score: 98.4
IPR014306Hydroxyisourate hydrolaseCDDcd05822TLP_HIUasecoord: 215..336
e-value: 2.01404E-48
score: 156.547
IPR018020Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylasePFAMPF09349OHCU_decarboxcoord: 7..158
e-value: 3.7E-32
score: 112.0
IPR036778Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase superfamilyGENE3D1.10.3330.10coord: 8..164
e-value: 3.0E-52
score: 179.1
IPR036778Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase superfamilySUPERFAMILY158694UraD-Likecoord: 12..162
NoneNo IPR availablePANTHERPTHR10395:SF75-HYDROXYISOURATE HYDROLASEcoord: 6..336
IPR023418Transthyretin, thyroxine binding sitePROSITEPS00768TRANSTHYRETIN_1coord: 220..235
IPR023419Transthyretin, conserved sitePROSITEPS00769TRANSTHYRETIN_2coord: 319..331

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G378Cucsat.G378gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G378.T4.E1Cucsat.G378.T4.E1exon
Cucsat.G378.T4.E2Cucsat.G378.T4.E2exon
Cucsat.G378.T4.E3Cucsat.G378.T4.E3exon
Cucsat.G378.T4.E4Cucsat.G378.T4.E4exon
Cucsat.G378.T4.E5Cucsat.G378.T4.E5exon
Cucsat.G378.T4.E6Cucsat.G378.T4.E6exon
Cucsat.G378.T4.E7Cucsat.G378.T4.E7exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G378.T4.C6Cucsat.G378.T4.C6CDS
Cucsat.G378.T4.C5Cucsat.G378.T4.C5CDS
Cucsat.G378.T4.C4Cucsat.G378.T4.C4CDS
Cucsat.G378.T4.C3Cucsat.G378.T4.C3CDS
Cucsat.G378.T4.C2Cucsat.G378.T4.C2CDS
Cucsat.G378.T4.C1Cucsat.G378.T4.C1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G378.T4Cucsat.G378.T4-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006144 purine nucleobase metabolic process
biological_process GO:0019428 allantoin biosynthetic process
biological_process GO:0009742 brassinosteroid mediated signaling pathway
biological_process GO:0001560 regulation of cell growth by extracellular stimulus
cellular_component GO:0005829 cytosol
cellular_component GO:0031234 extrinsic component of cytoplasmic side of plasma membrane
cellular_component GO:0005777 peroxisome
molecular_function GO:0033971 hydroxyisourate hydrolase activity
molecular_function GO:0051997 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase activity
molecular_function GO:0042802 identical protein binding