Cucsat.G19871.T4 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G19871.T4
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionERAD-associated E3 ubiquitin-protein ligase HRD1B-like
Locationctg4: 2363456 .. 2371361 (+)
RNA-Seq ExpressionCucsat.G19871.T4
SyntenyCucsat.G19871.T4
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATACCTATCTTTCTACATTCTTCATCTTTTCCAGATTTTCGGTTTTGTTCTCAGATCGCTCTCTACTTTCGTTACCTTCTCAGGTACTCTCCATCTTCTTCTATCTCTTTCTACAAGTCATTGTTGAACTTCGTTAGGCGACAGACGAAATGGAGCCTAAATTGCCTAATGTCTTCTGTTTTTTGTTGCAGTAAATTGGAAATTTATGTTTGGTATGAATGTTTTCTTTTGCTCGTGATTTCTGCTTCAGAATTTAGGGCTTTGCAATTGATTACGATTAATTTATGTTAGGCTTCGTATTTGGATTAGAACAAGCTCTCGGCTCTCGATAAGCTTTTGGTTATAGGTTTGGAGCTTGGCACATTGGGTTTTTTTTTCCCTTCTGATTTTCCTTTATTCTTCGTCTTAGCAATTGGAGTCCTTTGTTCTTTTTGGAATTTTTATGTATTGATCGTTGCTTGATAAGCTTGTGGTTTAGAAGTCGAGTGGTAAACATCAATGGTGAAAAGATGTATTTTTCTTAATCTTTATTTATTTATTTCTTTTGGAATTCAAACAAATGCTAGTTTCGTTGGTTCTTCTATCAACATTCCCTTCTCCAAGAAAAAACTCTTGAACCCCTTGAAGTGACCTCTTTATCATTTTTTTTTTGCTAAAAGGACTTTTGATTCCTTATCCCCGTAACCATTTTGTTTTGGAAAATTAAAGCTATAGATACTGCTTCCATCTCCAAATTTCTTACTTTGTTTTCTACTTTTTACCAATTGTTTATAAAACCAAGCCAAAACTTGAAAACTAAAAAAAGCAGCTTTCAAAAATTCGTTTTAGTTTTTGAAATTTGGCTAAGAATGCAACCATTGAAGATGCTAATCACTGTAAGAGATTGGGAGGAAATATGCTTGATTTTTCAAAAACGAAATATTACCAAATGGGGCTTTAATTTTCCAAATACACACGTTCCTTTGCAATCAATGTTCGTCAATGGTGATTGCCCAAATTATCCTTAAAAAACTCTTTGGTCGTTTCTTGCAAATGTCCTTTTATCTTATCATAAGTGCATGGTAACGATGATAATGAAACAATTTCGACTCTCTTTATGCATTATTCTCAGTTTAAATGTCTTACTGAAAGATAACTAATTCATAATATTTTCAAGATAGGTAACGATGTTATTTTAGCATCATTTAATTGTTTCATTTGGCTGACGTTTCAACTTCTCATATGGCAGTGCGGTAGAGAATGATGAAGCTGCAAACATATGCGGGGATTAGTTTTATTGCAACCATAATGGTTATCTATCATGCCTTCAACAGTAGAGGGCAGTTCTACCCAGCATTGGTCTATTTATCGACCTCCAAGATAAATCTAGTACTTCTTCTCAATATGGGTTTAGTCATCATGTGCATTTTGTGGCAGCTAACAAAAAAAATATTTCTGGGTTCTCTTAGAGAAGCAGAAGTTGAGAGGCTCAACGAGCAATCATGGAAGGAGTTGATGGAAATCCTCTTTGCCATCACTATTTTTCGGCAAGATTTTTCTGTTGCATTCCTGGGTATGGTTACGGCTCTGTTGTTGATCAAAGCTCTCCATTGGCTGGCTCAGAAGAGAGTTGAATACATGGAAACCACTCCATCGGTGCCATTGTTGTCTCATATCCGGATTGTTTCTTTCTTAGGCTTCCTCTTTGTTCTCGATAGTCTGTTTTTTTATAATTCGATAGATTCTCTGATACAGACAAGGAAGGCCTCGGTTTCCCTCTTCTTTTCATTTGAGTAAGTTTTCAATTTCCATTGGTTCATATCTTTTTCTTTCCTTTTTGTGGTCATAGTTGTTGTTATAGGAAATATACATATCATTATTATGTTGAAATTACAAAAGAGGAAAGTCCTATACAATGGATTTCAAAATGCTTTTCCAATTGACCATGAAATTTGGTTCCTATTTTGATTCTGTATTTAGACAAGTATTAGTTATGATGTTTCATAATGAAAATTTTTGAACATTTGGTACCACATTAATTTCATGGAGGTGTGGTTAGAATGTCCGAGATACCAAGGTCATGTTAGATGAACTCACAAAATTATTGTTTAAATTTGATACTTAAGTTCTTATATAGAGAATTTATGGCTCTGTCTTGTAGTCTTTGCTTGCAAGAGTTAGGTTTTTAAATATCCGATTATGCTTCTTGTGTCCATGAGAGGGTCTTATTTAAACATAAAATAAATTCAATGACTTGTTTCAGGTATATGATTCTTGCGACAACAGCTGTGTCAACATTTGTGAAATATATCTTCTATGTCAGTGATGTGCTCATGGAGGGACAATGGGAAAGGAAACCAGTCTATACATTTTACTTGGAACTTATTCGAGACTTGCTTCACTTGTCGATGTACTTGTGTTTCTTTCTTGTGATTTTCATGTAAGTAATCAAACTACACCCATGAGCTATCTCATTCTTGCGTACTGGAATTTCAGAATTAATCTCTTTCTGCATAAATGTTCTCATACTGCCGATATCTAAATGGGATTCTGATTCTTCTCAAGAAATACTATGCAAACCAAAAAAGTCAGATGTGGTTACTAGGATTCGTGTCCTTTCATGTATCAATGGCTCTCATATTTTTCTTTCATGTTAATTGTGGGAGATGGCTCATAAGTCATAGTAGGAGTGTCTTAAGAATGATAGGGTATTTCTTGTTTGTTTGGATATCCTTTACGTACCTATTATCAATCAGTTGTTTTAGAATTTTACTCCCTTAAAGATTGAAAGAGGCAGAGATTTCTGTTTACATGCACACATTGCACAAAAAAAGAATGATTTTGCTTGTAGTCTGACCAATTAATTTTTGGATATTGCTAGACTGTAAACATGGTGGAAAGTTTAGAATTCTTTTCTTTTATATGTCTGTCTTTTGAATGTGTTGTTTTCATCATCACACACACACACACATAGATATGGTCAAGTATATTTTTACACCTACTGTTTAATAATAAGGAAATAAGGAAGCTAACTAAAGCTCAAGGACAAGTTTAATGTACAACTTCCTTGTCTCTATATGCATGATGTAGCACAATGGGATAGGACATCAATCGCCATCCCATTGATGGTTTGATCCCCTACCCATGGTTGTTGAACTTTAAATAATATTTGGAGTTACATCCACACTAACTTCATTCCCATTTCATATCTTTACTGTACTGACTTTTGACTTTGCTCTTGTAGGACTTATGGTGTGCCTTTGCACTTGATCCGAGAGCTTTATGAGATCTTTAGGAATTTCAGGATTCGCATTGCAGATTACATCCGTTATAGAAAGATTACTTCAAACATGAACGATCGTTTTCCAGATGCAACGCCTGAAGAACTCAATTCGTGCGTCCAAGCTTGGTCAACTTGTTGTGTCTTTGATGCTTCCAGTATTTTTATGTTAGCTACTAAAGTTGTTTTTATGGAATTGCAGAAGTGATGCAACATGCATTATTTGTCGTGAAGAGATGACAGTTGCAAAGAAACTAGTATGTGGACACCTTTTTCATGTCCATTGTCTCCGATCTTGGTTGGAGCGACAACATACTTGTCCCACTTGTAGAGCCCTTGTTGTACCACCTGAAGGTTCAAGTACAACAAGCGGTCATCATGGAGTGCAGTCTGATGCTAACCAACAAGGTAATTGCACTTGTAACAATTCAATTCAATTTACTGCCTGGCTAAAGGGTCCTACGTCTTGTACTATTTTTAATCTGGTAAGTTTTATTAATTTCCTCCATGTGTATTTTATCTGGCTGTGGACCTTTCTGGAATCTTTAGTATGGAGAAAGTCTAAAAACTCTAGAATTCTGAAAATTTTGGACTGCATAATTATTTTTAATTGGCTTAAAATACCCCTTAAATGGGCGGTTGATAGTGGGTCTTGTTTCACTATTTATTTTTTATTCTTATTTTTCTGTACATGGAGTGCCCTTGGTGTGCTGATGGTGTGTCTTAGCATGTTAAGATACAAATAAATAATAATAAAATAGTGGACACACCCATGATAGTATTCGATGTATGTGTAAATTAGCCCCGACACTCACAAATAAATATAAAAAAAATAACGGACATGCCTAACACGTGTCCACACTGATACTTCTCCGAGCTTAGAAGTGTCGGTGCTTTATAAGTATCAGATTTGGTTTAACGTTTCAAAACTATTAAATAATCTAGATCATGAAGCAACCATTAAAAAATAAGCTATCAATATTTGCAAGTATTCTCTGTATATAATTATATGCCCCACCATCAGTGTGAAAGTTGAAATTTGAGCCTCTCAATTCATTGCATGGGTGTTGACTCTCTCTACTCCCTTCCCTCTTCTTATTTCTTTTGTTTTCATGATTGTTCTTCTTAAGAGACTGTCGTTAAAGTTGAAGTTTGTTTTATATATTTTTGTTTCTTAGCAAATTGCATTCACTCAGACCTAAAAGAAGCAGTTTCATTCTAGAACTGTATGAACTTAAGCATATTGAGAATGGATGATCATTATTGCTGAAAATGCTTTCTTTACCTAAAAAACTGTTTGAAGTCTTTTATACAATCTCATTTCTTTGGATTAACAACATTAAATGTTTGGTTGTTTTGTGAATGCTTGTAGGACTCCATTTAGTGTATCTCTGCTTTCTCTAATGGACGTCTTTGTTGCAGATATAGAACTGTTGGCTTCATTTAATTGTTTTTTCATGATGGAGGTATACCATGCTGAAACCTGGATACAATGACTGAGCTGTTTATTATGTTGATAGCAGGAACAGGAACCACAAGTTCATCTGCACAAGGTACAAGTGGCACGGGCGTTACTAATGATAATTTAAGTCATCATCAAGCTAGAGTCCGAGCTGCTGCCGCCGCTGCTTCAATATATGAGAAATCGTATGTTTATCCTTGTGCGAGCACATTAGTATGGTATGTCTTTTCATTACAAACTATGTGATCTCTGTTTTTTCTCCTTTATGTTGGTCTAGAAAGTTTACAAACATTGAGGCCATTTCTCCCTTTGCAAAGAGAAGGAAGAGTATGAAATTTTATTATTATAATTATTATAATTTGTTTATTTGTGTACAGGTCTCTTGGATATGCAGTAGTTCCTCAAGTTGAAAGACCTTTGTTTGACTCTAACAATACAGAAACAAATGGAGAGCAATCTCCCAATGGACATCCACAATACTTTTCATTTCCTGGCGGCCCTTCAAACCTGTCCTTCACTCAATTTCCCCAAAATATCTTTGTTCCTTTTCAGCCACGAGAGGGCACTGGAAACAATATAGAGGAATTGGGCAGTAGTTTAAATGTATCAGGCTCACAGCTTGAAGCTCAAGAAAAATTTCTTCAACAACAGATTGAGGTATGTTATCAACGTTACCTAGTGAGCTGTTTTGCTTGGTTAGTGCATGTTTTATTTAGTCTCTTGTTGCCGGGCCCTTGTATAATCTTCAGCTCTGCTTTTGAAGGAAAAAAGAAAAAAAAAACGTGAACAGTAGTTGACTAGTATGCACACATGGCCTTGACCATGGGGAAATAATACTCCTGAATCAATATCATTTCTCATACCCTTTTTTTTAAAAAAAATCATTTCTCATACCCTTTTCTTAAAAAAAAATCATTTCTCATACTTAGGTGATGTTTATGATGAACTCAAATGATCAGAATAACATGTCTTGATTCTCTCGTTGTCCTGGATTGACCAAACCGCTGTTTGGTTTTCCTATTTGTGCTGTATCCTGGACCTCCTGTGTCAACAACAAGAGACTGCTTTGATGTTTGCGGTTCAAAATTGGAGTACAAAAATAACTGGGCCGACCAAGATGAGGTACCCTTAACAGTTAAAAAGAAAAAACACCATTAGCAGCCCTAGTTGTTTGTGATCAAGAGATTAGGACCATTACAAGAGGGTTCGAAGTAAGCTCTCCAGAGAGAGTTATAAAATGTGTCAAATCTCAGACCTTCCTTTGAAACCAATTTATTTCTAAGAAAAACTTCCTGTGTATTCGCAACAACTATCCTTAGAATGGCAACAAATTCTACTTTCTTTAATAATTAGTCTCTATGTTGGAAATTGGGTTCAAGAAAGCTTTTCCACCATTATGTGAATAGTTGAAGGAACTCTCAAAATGCCAAAGTGCGCTTGGCTCAGTGGCGTACCTGGAGGTTAGAAGTTTAATGCCCCGTACCGTAGTTGTGCTAAAAGAAAAAATCTTCTGAGGCAGACTTACACACAGATTCTTTGGATAAAGAACTAGGTTTTAATCTTCCAAAGGAAGTATGTCTTTTGAGGATTTTCAAAATCCTAGTTTTTTTAGAAAAGAGGATACGGGGAGGAAAGAAAGGAATAAATTATGCAGAAGACTTTTCGATGGAAACACCATAAGAATGAAGGGATCACAATCAATGATTGGGAGCCCAAACATAACTTTTACTCTTTGGATAGAAGAGAATATCAGTGGAAGGAGGCTACTTATTGACTTCTCTATTGCAAAGGGCTTTATGAAAATGAAAATAAGAGGAAAGAGACCCACTTCTAGAGTTCTAGCTAACGAACTATTTGGAAATCTTTGACTAGGGATAAGAAATACCGATGCAAAAATTGCACCCAGAGGGTACTATTTGGAAGCCAATCATCTTCCTTCAATCAAACTATGCAACAATCCCTCACAATTACTTACACATAAGATAGAAAGCGAAGATGTGTAGATCAAAGGAGGAGGCCTTTTATATTCTCCTTAACATCTCCTTAACAGCCCTTGGACTTGTGCATTATAATAAGTCATGCAGGATCTCCTTGAAAACTACCTAAATCAGTGCGTCGACTCTTTGATGTAAGCCATGTGGATTGAGTTCAGGAAATACTTAACGTCTTGGAGAGAAGCCTATTCTTTTAAGCCCTTGGATATTTATCCTCCATAGATTGCACACAACTTCTCCTCACCGGCCCTTGGGAATTTCATGGAAATTCGTATGGACTCGGTTCAAAAAGATACTTTTTTGTATCTCTCCAAGTCCTTGGAGTTGTATATGCTTTGTAGATCCCTTCCAATTTTTCAATTAAAGGTTGAGTTGAGGAGATGCTTGTAGTTTTGATGGAAGAAATAAATATATAAACTTGATTTTAGATTTTTGTTCAAAAAGGGTTAAAGTTCTTCCAAACAACTCTATATTTTCTAGAAACTAGAGGTTAGAAATGATAAGTTAAGGAGGCATCTTCATAATAAATTAATTTGTACTCGATTCAATTTCAAACTAATTTCAGTAGAACAGAGCTAGGCGTAGTTGAGGGTTTTTACGAAATCAATACCTTTTTCCTTCTATTAAATTCAAAATTTCGTATTTGCCAACAGTTCCTTCAGAATCAACTGCAACTTCTCCGTAAACCAAAAGTTGAGGAAAGCTCAAGAGGAGCTAGCGAGGCAGATAAAAAAGGGAAAAGTTTAGCTATCTCACCATCATCGTCGGCGTCATTGTCTTCATCAGATTCTCATCCACCTGGAGAAACCGAAAGGGCGGTAAGATAGAATAAATGTGCATAATAATATAGTGTAATAATACGAGACTGCAGATCGACACAATGATGACATAGATATGTGTCTATTGTATACGGAAGATGTACTTGGTAACTTTTTAGAAAGCAGTCTTCCTCCCTTTTGCCATGTTGGTGGTTGGTTCGGTTTGGTTGTAAATGCTGAATTTGGTCTCTTTTAAAGCAATCGATGCGATGGTTAAGAAATGGCTTCTTTTTTTCTTTAATATCGTAGCAAAAGAAGAGAGTTTTTATTCAAAGATGTTTGGTTGTTTCTATTCTCTTAGTCTAAAAGATGCTTTTCTTGAAAAGCAATTAGATTGACATGCGCATGAATGAAAAGGCTTTGATTGTTTG

Coding sequence (CDS)

ATGATGAAGCTGCAAACATATGCGGGGATTAGTTTTATTGCAACCATAATGGTTATCTATCATGCCTTCAACAGTAGAGGGCAGTTCTACCCAGCATTGGTCTATTTATCGACCTCCAAGATAAATCTAGTACTTCTTCTCAATATGGGTTTAGTCATCATGTGCATTTTGTGGCAGCTAACAAAAAAAATATTTCTGGGTTCTCTTAGAGAAGCAGAAGTTGAGAGGCTCAACGAGCAATCATGGAAGGAGTTGATGGAAATCCTCTTTGCCATCACTATTTTTCGGCAAGATTTTTCTGTTGCATTCCTGGGTATGGTTACGGCTCTGTTGTTGATCAAAGCTCTCCATTGGCTGGCTCAGAAGAGAGTTGAATACATGGAAACCACTCCATCGGTGCCATTGTTGTCTCATATCCGGATTGTTTCTTTCTTAGGCTTCCTCTTTGTTCTCGATAGTCTGTTTTTTTATAATTCGATAGATTCTCTGATACAGACAAGGAAGGCCTCGGTTTCCCTCTTCTTTTCATTTGAGTATATGATTCTTGCGACAACAGCTGTGTCAACATTTGTGAAATATATCTTCTATGTCAGTGATGTGCTCATGGAGGGACAATGGGAAAGGAAACCAGTCTATACATTTTACTTGGAACTTATTCGAGACTTGCTTCACTTGTCGATGTACTTGTGTTTCTTTCTTGTGATTTTCATGACTTATGGTGTGCCTTTGCACTTGATCCGAGAGCTTTATGAGATCTTTAGGAATTTCAGGATTCGCATTGCAGATTACATCCGTTATAGAAAGATTACTTCAAACATGAACGATCGTTTTCCAGATGCAACGCCTGAAGAACTCAATTCAAGTGATGCAACATGCATTATTTGTCGTGAAGAGATGACAGTTGCAAAGAAACTAGTATGTGGACACCTTTTTCATGTCCATTGTCTCCGATCTTGGTTGGAGCGACAACATACTTGTCCCACTTGTAGAGCCCTTGTTGTACCACCTGAAGGTTCAAGTACAACAAGCGGTCATCATGGAGTGCAGTCTGATGCTAACCAACAAGGAACAGGAACCACAAGTTCATCTGCACAAGGTACAAGTGGCACGGGCGTTACTAATGATAATTTAAGTCATCATCAAGCTAGAGTCCGAGCTGCTGCCGCCGCTGCTTCAATATATGAGAAATCGTATGTTTATCCTTGTGCGAGCACATTAGTATGGTCTCTTGGATATGCAGTAGTTCCTCAAGTTGAAAGACCTTTGTTTGACTCTAACAATACAGAAACAAATGGAGAGCAATCTCCCAATGGACATCCACAATACTTTTCATTTCCTGGCGGCCCTTCAAACCTGTCCTTCACTCAATTTCCCCAAAATATCTTTGTTCCTTTTCAGCCACGAGAGGGCACTGGAAACAATATAGAGGAATTGGGCAGTAGTTTAAATGTATCAGGCTCACAGCTTGAAGCTCAAGAAAAATTTCTTCAACAACAGATTGAGTTCCTTCAGAATCAACTGCAACTTCTCCGTAAACCAAAAGTTGAGGAAAGCTCAAGAGGAGCTAGCGAGGCAGATAAAAAAGGGAAAAGTTTAGCTATCTCACCATCATCGTCGGCGTCATTGTCTTCATCAGATTCTCATCCACCTGGAGAAACCGAAAGGGCGGTAAGATAG

Protein sequence

MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQLTKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLAQKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYMILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYGVPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMTVAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTTSSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVERPLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGSSLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASEADKKGKSLAISPSSSASLSSSDSHPPGETERAVR
Homology
BLAST of Cucsat.G19871.T4 vs. ExPASy Swiss-Prot
Match: Q6NPT7 (ERAD-associated E3 ubiquitin-protein ligase HRD1B OS=Arabidopsis thaliana OX=3702 GN=HRD1B PE=2 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 4.6e-172
Identity = 334/535 (62.43%), Postives = 394/535 (73.64%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           M++L+ YAG+S +AT++VIYHAF+SRGQFYPA VYLSTSKINLV+LLNMGLV+M  LW L
Sbjct: 1   MIQLKVYAGLSTLATLVVIYHAFSSRGQFYPATVYLSTSKINLVVLLNMGLVLMLSLWNL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
            K +FLGSLREAEVERLNEQ+W+ELMEILFAITIFRQDFSV F+ +V  LLLIK LHW+A
Sbjct: 61  VKIVFLGSLREAEVERLNEQAWRELMEILFAITIFRQDFSVGFISLVVTLLLIKGLHWMA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180
           QKRVEY+ETTPSV LLSH+RIVSF+ FL +LD L  Y+SI  LIQ+RKAS+S+FF+FEYM
Sbjct: 121 QKRVEYIETTPSVTLLSHVRIVSFMVFLLILDCLLTYSSIQQLIQSRKASMSVFFTFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATT VS  VKY FYV+D+L EGQWE KPVYTFYLEL+RDLLHLSMYLCFFL+IFM YG
Sbjct: 181 ILATTTVSIIVKYAFYVTDMLKEGQWEGKPVYTFYLELVRDLLHLSMYLCFFLMIFMNYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           +PLHLIRELYE FRNF+IR+ DY+RYRKITSNMNDRFPDATPEEL+S+DATCIICREEMT
Sbjct: 241 LPLHLIRELYETFRNFKIRVTDYLRYRKITSNMNDRFPDATPEELSSNDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPE-GSSTTSGHHGVQSDANQQGTGT 360
            AKKLVCGHLFHVHCLRSWLERQ+TCPTCRALVVP E  +ST SG+ G   ++ QQGTGT
Sbjct: 301 SAKKLVCGHLFHVHCLRSWLERQNTCPTCRALVVPAENATSTASGNRGPHQESLQQGTGT 360

Query: 361 TSSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYP-CASTLVWSLGYAVVPQV 420
           +SS  QG+S +   ++N+S H+AR +AAA+AASIY +S VYP  A+TLVWS G +++PQ 
Sbjct: 361 SSSDGQGSSVSAAASENMSRHEARFQAAASAASIYGRSIVYPSSANTLVWSQGNSLLPQT 420

Query: 421 ERPLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEEL 480
           E                                                           
Sbjct: 421 E----------------------------------------------------------- 457

Query: 481 GSSLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASEADKKGKSLA 534
                     +EAQ +FL+ QIE L NQL+LL KP         +  D KGKS+A
Sbjct: 481 ----------VEAQRRFLESQIEVLTNQLRLLEKP---------TTVDTKGKSVA 457

BLAST of Cucsat.G19871.T4 vs. ExPASy Swiss-Prot
Match: Q9LW77 (ERAD-associated E3 ubiquitin-protein ligase HRD1A OS=Arabidopsis thaliana OX=3702 GN=HRD1A PE=2 SV=1)

HSP 1 Score: 602.4 bits (1552), Expect = 5.1e-171
Identity = 338/536 (63.06%), Postives = 403/536 (75.19%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           M++L+TYAG+SF+AT+ VIYHAF+SRGQFYPA VYLSTSKI+LVLLLNM LV+M  LW L
Sbjct: 1   MIRLRTYAGLSFMATLAVIYHAFSSRGQFYPATVYLSTSKISLVLLLNMCLVLMLSLWHL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
            K +FLGSLREAEVERLNEQ+W+ELMEILFAITIFRQDFS  FL +V  LLLIKALHWLA
Sbjct: 61  VKFVFLGSLREAEVERLNEQAWRELMEILFAITIFRQDFSSGFLPLVVTLLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180
           QKRVEY+ETTPSV  LSH RIVSF+GFL ++DSLF Y+SI  LIQ+R+ASVSLFFSFEYM
Sbjct: 121 QKRVEYIETTPSVSKLSHFRIVSFMGFLLLVDSLFMYSSIRHLIQSRQASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATT V+ FVKY+FYV+D+LM+GQWE+KPVYTFYLELIRDLLHLSMY+CFF VIFM YG
Sbjct: 181 ILATTTVAIFVKYVFYVTDMLMDGQWEKKPVYTFYLELIRDLLHLSMYICFFFVIFMNYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHL+RELYE FRNF+IR++DY+RYRKITSNMNDRFPDATPEEL +SDATCIICREEMT
Sbjct: 241 VPLHLLRELYETFRNFQIRVSDYLRYRKITSNMNDRFPDATPEELTASDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360
            AKKL+CGHLFHVHCLRSWLERQ TCPTCRALVVPPE +++ +         +QQ  GT+
Sbjct: 301 NAKKLICGHLFHVHCLRSWLERQQTCPTCRALVVPPENATSAAPGQRELHQGSQQ--GTS 360

Query: 361 SSSAQG---TSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQ 420
           SS  QG   +S  GV+N++LS H AR++AAA+AAS+Y KS VYP A+T+ WS G   VP 
Sbjct: 361 SSGNQGSEISSSAGVSNNSLSRHHARLQAAASAASVYGKSMVYPSANTVAWSSG---VPG 420

Query: 421 VERPLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEE 480
            E+   + + T           PQ+        NL                      +E 
Sbjct: 421 TEQVSTEPDQT----------LPQH--------NLP---------------------VEN 480

Query: 481 LGSSLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASEADKKGKSLA 534
             +  N+S ++LE   K L+  +E L+N+L  L   K E     A E + KGKS+A
Sbjct: 481 SHAYANMSETKLEEMRKSLETHLEILRNRLHFLETRKPE----SAGEPENKGKSVA 488

BLAST of Cucsat.G19871.T4 vs. ExPASy Swiss-Prot
Match: Q5Z880 (ERAD-associated E3 ubiquitin-protein ligase HRD1 OS=Oryza sativa subsp. japonica OX=39947 GN=HRD1 PE=2 SV=1)

HSP 1 Score: 562.0 bits (1447), Expect = 7.6e-159
Identity = 314/514 (61.09%), Postives = 377/514 (73.35%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           M++LQTYA  S +AT   +Y+AF+SR QFYPA+VYLSTSKI  VLLLN GLV MC+ WQL
Sbjct: 1   MIRLQTYAAFSLMATATAVYYAFSSREQFYPAMVYLSTSKICFVLLLNTGLVAMCVAWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
            K++FLG+LREAEVERLNEQ+W+E++EILFA+TIFRQDFSV+FL MV ALLL+KALHWLA
Sbjct: 61  VKRLFLGTLREAEVERLNEQAWREVVEILFAVTIFRQDFSVSFLAMVAALLLVKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180
           QKRVEY+ETTPSVP+LSH RIVSF+ FL V+D LF  NS+ SLI  R+ASV++FFSFEYM
Sbjct: 121 QKRVEYIETTPSVPMLSHARIVSFMLFLLVVDCLFLSNSLRSLIHKREASVAIFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILAT+ VSTFVKYIFYVSD+LMEGQWE+K VYTFYLELI DL+HLS+Y+ FF+ IF+ YG
Sbjct: 181 ILATSTVSTFVKYIFYVSDMLMEGQWEKKAVYTFYLELISDLVHLSLYMLFFIAIFLNYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYE FRNFRIRIADY+RYRKITSNMN+RFPDAT +ELN+SDATCIICREEMT
Sbjct: 241 VPLHLIRELYETFRNFRIRIADYVRYRKITSNMNERFPDATADELNASDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360
            AKKL+CGHLFHVHCLRSWLERQHTCPTCRA ++PP+   T +  HGV         G  
Sbjct: 301 TAKKLLCGHLFHVHCLRSWLERQHTCPTCRAPILPPDNGRTAARPHGVH-------PGVQ 360

Query: 361 SSSAQGTSGT-GVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVE 420
                GT G+     +N+S  QA++ AAA+AAS+Y +S+ YP A+ L     Y+  PQ  
Sbjct: 361 PVPGNGTPGSERAAGENISRRQAKLEAAASAASLYGRSFAYPPANNL---NRYSTPPQST 420

Query: 421 RPLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGN--NIEE 480
                S    T+  QSP GH    + P  P+              F  R    +     E
Sbjct: 421 SNGPQSGEASTS-NQSPKGHAT--ADPSAPT--------------FYARGAVSSVTTTRE 480

Query: 481 LGSSLNVSGSQLEAQEKFLQQQIEFLQNQLQLLR 512
           L SSL       +A E  ++ QIE LQ QLQ+ +
Sbjct: 481 LESSLQ------KAYENAIKSQIEMLQIQLQMFQ 481

BLAST of Cucsat.G19871.T4 vs. ExPASy Swiss-Prot
Match: Q803I8 (E3 ubiquitin-protein ligase synoviolin OS=Danio rerio OX=7955 GN=syvn1 PE=2 SV=2)

HSP 1 Score: 262.3 bits (669), Expect = 1.2e-68
Identity = 145/353 (41.08%), Postives = 208/353 (58.92%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           M++       S   T  V+ HA+  + QFYP +VYL+ S  ++ +L     V++ +L +L
Sbjct: 1   MVRAALVTATSLALTGAVVAHAYFLKHQFYPTVVYLTKSSPSMAVLYIQAFVLVFLLGKL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
            +K+F G LR AE+E L E+SW  + E   A T+FR DFS  F+ + T LL +K  HWLA
Sbjct: 61  MRKVFFGQLRAAEMEHLIERSWYAVTETCLAFTVFRDDFSPRFVALFTLLLFLKCFHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180
           + RV++ME +P++  + H R++S +  L V+D LF  ++  S+I TR ASV L F FEY 
Sbjct: 121 EDRVDFMERSPNISWVFHFRVLSLMVLLGVMDFLFVNHACHSII-TRGASVQLVFGFEYA 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           IL T  ++TF+KY  +  D+  E  W+ K VY  Y EL    + + +Y+ F  ++   + 
Sbjct: 181 ILMTMVLTTFIKYTLHTIDLQSENPWDNKAVYMLYTELFTGFIKVLLYMAFMTIMIKVHT 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEM- 300
            PL  IR +Y   R F+  + D I  R+   NMN  +PDATPE+L ++D  CIICREEM 
Sbjct: 241 FPLFAIRPMYLAMRQFKKAVTDAIMSRRAIRNMNTLYPDATPEDLQATDNVCIICREEMV 300

Query: 301 TVAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDA 353
           T AKKL C H+FH  CLRSW +RQ TCPTCR  V+     + T      Q+ A
Sbjct: 301 TGAKKLPCNHIFHSSCLRSWFQRQQTCPTCRMDVLRASQPNQTPAPPAAQAPA 352

BLAST of Cucsat.G19871.T4 vs. ExPASy Swiss-Prot
Match: Q86TM6 (E3 ubiquitin-protein ligase synoviolin OS=Homo sapiens OX=9606 GN=SYVN1 PE=1 SV=2)

HSP 1 Score: 260.8 bits (665), Expect = 3.6e-68
Identity = 141/331 (42.60%), Postives = 198/331 (59.82%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           M +       S   T  V+ HA+  + QFYP +VYL+ S  ++ +L     V++ +L ++
Sbjct: 1   MFRTAVMMAASLALTGAVVAHAYYLKHQFYPTVVYLTKSSPSMAVLYIQAFVLVFLLGKV 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
             K+F G LR AE+E L E+SW  + E   A T+FR DFS  F+ + T LL +K  HWLA
Sbjct: 61  MGKVFFGQLRAAEMEHLLERSWYAVTETCLAFTVFRDDFSPRFVALFTLLLFLKCFHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180
           + RV++ME +P++  L H RIVS +  L +LD LF  ++  S++ TR ASV L F FEY 
Sbjct: 121 EDRVDFMERSPNISWLFHCRIVSLMFLLGILDFLFVSHAYHSIL-TRGASVQLVFGFEYA 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           IL T  ++ F+KY+ +  D+  E  W+ K VY  Y EL    + + +Y+ F  ++   + 
Sbjct: 181 ILMTMVLTIFIKYVLHSVDLQSENPWDNKAVYMLYTELFTGFIKVLLYMAFMTIMIKVHT 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEM- 300
            PL  IR +Y   R F+  + D I  R+   NMN  +PDATPEEL + D  CIICREEM 
Sbjct: 241 FPLFAIRPMYLAMRQFKKAVTDAIMSRRAIRNMNTLYPDATPEELQAMDNVCIICREEMV 300

Query: 301 TVAKKLVCGHLFHVHCLRSWLERQHTCPTCR 331
           T AK+L C H+FH  CLRSW +RQ TCPTCR
Sbjct: 301 TGAKRLPCNHIFHTSCLRSWFQRQQTCPTCR 330

BLAST of Cucsat.G19871.T4 vs. NCBI nr
Match: XP_004139202.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1B isoform X2 [Cucumis sativus] >KGN60852.1 hypothetical protein Csa_019293 [Cucumis sativus])

HSP 1 Score: 1061 bits (2745), Expect = 0.0
Identity = 557/558 (99.82%), Postives = 558/558 (100.00%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTT+GHHGVQSDANQQGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTTGHHGVQSDANQQGTGTT 360

Query: 361 SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420
           SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER
Sbjct: 361 SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420

Query: 421 PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480
           PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS
Sbjct: 421 PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480

Query: 481 SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASEADKKGKSLAISPSSSA 540
           SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASEADKKGKSLAISPSSSA
Sbjct: 481 SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASEADKKGKSLAISPSSSA 540

Query: 541 SLSSSDSHPPGETERAVR 558
           SLSSSDSHPPGETERAVR
Sbjct: 541 SLSSSDSHPPGETERAVR 558

BLAST of Cucsat.G19871.T4 vs. NCBI nr
Match: XP_031737096.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1B isoform X1 [Cucumis sativus])

HSP 1 Score: 1057 bits (2733), Expect = 0.0
Identity = 557/559 (99.64%), Postives = 558/559 (99.82%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQ-GTGT 360
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTT+GHHGVQSDANQQ GTGT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTTGHHGVQSDANQQAGTGT 360

Query: 361 TSSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVE 420
           TSSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVE
Sbjct: 361 TSSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVE 420

Query: 421 RPLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELG 480
           RPLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELG
Sbjct: 421 RPLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELG 480

Query: 481 SSLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASEADKKGKSLAISPSSS 540
           SSLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASEADKKGKSLAISPSSS
Sbjct: 481 SSLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASEADKKGKSLAISPSSS 540

Query: 541 ASLSSSDSHPPGETERAVR 558
           ASLSSSDSHPPGETERAVR
Sbjct: 541 ASLSSSDSHPPGETERAVR 559

BLAST of Cucsat.G19871.T4 vs. NCBI nr
Match: XP_008455400.1 (PREDICTED: ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucumis melo] >XP_016902283.1 PREDICTED: ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucumis melo])

HSP 1 Score: 1055 bits (2727), Expect = 0.0
Identity = 554/558 (99.28%), Postives = 554/558 (99.28%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360

Query: 361 SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420
           SSSAQGTSGTGVTN NLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER
Sbjct: 361 SSSAQGTSGTGVTNANLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420

Query: 421 PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480
           PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS
Sbjct: 421 PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480

Query: 481 SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASEADKKGKSLAISPSSSA 540
           SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASE DKKGKSLAISPS SA
Sbjct: 481 SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASETDKKGKSLAISPSPSA 540

Query: 541 SLSSSDSHPPGETERAVR 558
           SLSSSDSHPPGETER VR
Sbjct: 541 SLSSSDSHPPGETERVVR 558

BLAST of Cucsat.G19871.T4 vs. NCBI nr
Match: KAA0063647.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucumis melo var. makuwa] >TYK18378.1 ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucumis melo var. makuwa])

HSP 1 Score: 1053 bits (2722), Expect = 0.0
Identity = 552/555 (99.46%), Postives = 553/555 (99.64%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360

Query: 361 SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420
           SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER
Sbjct: 361 SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420

Query: 421 PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480
           PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS
Sbjct: 421 PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480

Query: 481 SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASEADKKGKSLAISPSSSA 540
           SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASE DKKGKSLAISP+ SA
Sbjct: 481 SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASETDKKGKSLAISPAPSA 540

Query: 541 SLSSSDSHPPGETER 555
           SLSSSDSHPPGETER
Sbjct: 541 SLSSSDSHPPGETER 555

BLAST of Cucsat.G19871.T4 vs. NCBI nr
Match: XP_038889876.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Benincasa hispida] >XP_038889877.1 ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Benincasa hispida])

HSP 1 Score: 1001 bits (2587), Expect = 0.0
Identity = 531/557 (95.33%), Postives = 538/557 (96.59%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           MMKLQTYAGISFIATI+VIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSI+SLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSST  GHHGVQSDANQQGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHHGVQSDANQQGTGTT 360

Query: 361 SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420
           SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPC STLVWSLGYAVVPQVER
Sbjct: 361 SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCPSTLVWSLGYAVVPQVER 420

Query: 421 PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480
           PL DS+NTETNGEQ+PNGHPQYFSFP GPSNLSFTQFPQN+FVPFQPREGTGNN +ELGS
Sbjct: 421 PLPDSDNTETNGEQTPNGHPQYFSFPSGPSNLSFTQFPQNLFVPFQPREGTGNNTDELGS 480

Query: 481 SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASEADKKGKSLAISPSSSA 540
           SLNVSGSQLEAQEKFLQQ IE LQNQLQLLR+PKVEESSR A EADKKGKSLA S S   
Sbjct: 481 SLNVSGSQLEAQEKFLQQ-IEILQNQLQLLRRPKVEESSR-AGEADKKGKSLATSSSPLP 540

Query: 541 SLSSSDSHPPGETERAV 557
           S SS DSHP GETERA 
Sbjct: 541 SSSSLDSHPSGETERAA 555

BLAST of Cucsat.G19871.T4 vs. ExPASy TrEMBL
Match: A0A0A0LJG4 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G013330 PE=4 SV=1)

HSP 1 Score: 1061 bits (2745), Expect = 0.0
Identity = 557/558 (99.82%), Postives = 558/558 (100.00%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTT+GHHGVQSDANQQGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTTGHHGVQSDANQQGTGTT 360

Query: 361 SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420
           SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER
Sbjct: 361 SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420

Query: 421 PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480
           PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS
Sbjct: 421 PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480

Query: 481 SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASEADKKGKSLAISPSSSA 540
           SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASEADKKGKSLAISPSSSA
Sbjct: 481 SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASEADKKGKSLAISPSSSA 540

Query: 541 SLSSSDSHPPGETERAVR 558
           SLSSSDSHPPGETERAVR
Sbjct: 541 SLSSSDSHPPGETERAVR 558

BLAST of Cucsat.G19871.T4 vs. ExPASy TrEMBL
Match: A0A1S4E231 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucumis melo OX=3656 GN=LOC103495570 PE=4 SV=1)

HSP 1 Score: 1055 bits (2727), Expect = 0.0
Identity = 554/558 (99.28%), Postives = 554/558 (99.28%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360

Query: 361 SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420
           SSSAQGTSGTGVTN NLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER
Sbjct: 361 SSSAQGTSGTGVTNANLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420

Query: 421 PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480
           PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS
Sbjct: 421 PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480

Query: 481 SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASEADKKGKSLAISPSSSA 540
           SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASE DKKGKSLAISPS SA
Sbjct: 481 SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASETDKKGKSLAISPSPSA 540

Query: 541 SLSSSDSHPPGETERAVR 558
           SLSSSDSHPPGETER VR
Sbjct: 541 SLSSSDSHPPGETERVVR 558

BLAST of Cucsat.G19871.T4 vs. ExPASy TrEMBL
Match: A0A5A7V9K3 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G001010 PE=4 SV=1)

HSP 1 Score: 1053 bits (2722), Expect = 0.0
Identity = 552/555 (99.46%), Postives = 553/555 (99.64%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360

Query: 361 SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420
           SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER
Sbjct: 361 SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420

Query: 421 PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480
           PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS
Sbjct: 421 PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480

Query: 481 SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASEADKKGKSLAISPSSSA 540
           SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASE DKKGKSLAISP+ SA
Sbjct: 481 SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASETDKKGKSLAISPAPSA 540

Query: 541 SLSSSDSHPPGETER 555
           SLSSSDSHPPGETER
Sbjct: 541 SLSSSDSHPPGETER 555

BLAST of Cucsat.G19871.T4 vs. ExPASy TrEMBL
Match: A0A6J1J826 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucurbita maxima OX=3661 GN=LOC111482600 PE=4 SV=1)

HSP 1 Score: 942 bits (2436), Expect = 0.0
Identity = 504/547 (92.14%), Postives = 515/547 (94.15%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           MMKLQTYAGISFIATI+VIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGL+IMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLIIMCILWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSV+FLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSI+SLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDAT EELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATSEELNSSDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSST +GHHG QSDANQQGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAAGHHGPQSDANQQGTGTT 360

Query: 361 SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420
           SSSAQGTS TGV NDNLSHHQARVRAAAAAAS+YEKSYVYPCASTLVWS GYAVVPQVER
Sbjct: 361 SSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAVVPQVER 420

Query: 421 PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480
           PL DSNN E      PNGHPQ FSF GGPSNLSFTQFPQN+FVPFQPREGTGNN + LGS
Sbjct: 421 PLPDSNNIE------PNGHPQQFSFTGGPSNLSFTQFPQNLFVPFQPREGTGNNTDGLGS 480

Query: 481 SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASEADKKGKSLAISPSSSA 540
           S NVSG  LEAQE+FLQQQIE LQNQLQ LRKP+VEESS GA EADKKGKS+A S  SS 
Sbjct: 481 SQNVSGLHLEAQERFLQQQIEILQNQLQHLRKPEVEESS-GAGEADKKGKSVAFSSLSSP 540

Query: 541 SLSSSDS 547
           S S S S
Sbjct: 541 SPSPSSS 540

BLAST of Cucsat.G19871.T4 vs. ExPASy TrEMBL
Match: A0A6J1CJF5 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011717 PE=4 SV=1)

HSP 1 Score: 941 bits (2431), Expect = 0.0
Identity = 503/559 (89.98%), Postives = 522/559 (93.38%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           MMKLQTYAGISFIATI+VIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180
           QKRVEYMETTPSVPLLSHIRIVSFL FLF+LDSLFFYNSI+ LI+TRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLSFLFILDSLFFYNSIEYLIRTRKASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATTAVSTFVKYIFYVSDVLMEGQWE+KPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSST  GHHG   DANQQGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHHGSH-DANQQGTGTT 360

Query: 361 SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420
           SSSAQ +SGTGV  DNLSHHQARV+AAAAAASIYEKSYVYPCASTLVWS GYAVVPQ +R
Sbjct: 361 SSSAQDSSGTGVAGDNLSHHQARVQAAAAAASIYEKSYVYPCASTLVWSPGYAVVPQTQR 420

Query: 421 PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480
           PL DSNN E NGEQ+PNG PQYF F GGP+NLSFTQFPQN+FVPFQPREG+ NN + LGS
Sbjct: 421 PLADSNNIEPNGEQAPNGQPQYFPFTGGPANLSFTQFPQNLFVPFQPREGSVNNTDGLGS 480

Query: 481 SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASEADKKGKSLAISPSSSA 540
           S NVSG  LE QE+F+QQQIE LQ QLQLLR PKVEES+R A EADKKGKS+A SP SS+
Sbjct: 481 SQNVSGLHLEDQERFIQQQIEMLQKQLQLLRNPKVEESTR-AGEADKKGKSVASSPPSSS 540

Query: 541 SLSSSDSHPP-GETERAVR 558
           S S S+SHPP GE ER VR
Sbjct: 541 S-SVSESHPPAGEIERMVR 556

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6NPT74.6e-17262.43ERAD-associated E3 ubiquitin-protein ligase HRD1B OS=Arabidopsis thaliana OX=370... [more]
Q9LW775.1e-17163.06ERAD-associated E3 ubiquitin-protein ligase HRD1A OS=Arabidopsis thaliana OX=370... [more]
Q5Z8807.6e-15961.09ERAD-associated E3 ubiquitin-protein ligase HRD1 OS=Oryza sativa subsp. japonica... [more]
Q803I81.2e-6841.08E3 ubiquitin-protein ligase synoviolin OS=Danio rerio OX=7955 GN=syvn1 PE=2 SV=2[more]
Q86TM63.6e-6842.60E3 ubiquitin-protein ligase synoviolin OS=Homo sapiens OX=9606 GN=SYVN1 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
XP_004139202.10.099.82ERAD-associated E3 ubiquitin-protein ligase HRD1B isoform X2 [Cucumis sativus] >... [more]
XP_031737096.10.099.64ERAD-associated E3 ubiquitin-protein ligase HRD1B isoform X1 [Cucumis sativus][more]
XP_008455400.10.099.28PREDICTED: ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucumis melo]... [more]
KAA0063647.10.099.46ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucumis melo var. makuwa... [more]
XP_038889876.10.095.33ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Benincasa hispida] >XP_0... [more]
Match NameE-valueIdentityDescription
A0A0A0LJG40.099.82RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G013330 P... [more]
A0A1S4E2310.099.28ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucumis melo OX=3656 G... [more]
A0A5A7V9K30.099.46ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucumis melo var. maku... [more]
A0A6J1J8260.092.14ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucurbita maxima OX=36... [more]
A0A6J1CJF50.089.98ERAD-associated E3 ubiquitin-protein ligase HRD1B-like isoform X1 OS=Momordica c... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 486..506
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 532..550
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 424..449
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 340..375
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 513..527
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 512..558
NoneNo IPR availablePANTHERPTHR22763:SF174SUBFAMILY NOT NAMEDcoord: 1..458
NoneNo IPR availablePANTHERPTHR22763RING ZINC FINGER PROTEINcoord: 1..458
NoneNo IPR availableCDDcd16479RING-H2_synoviolincoord: 289..330
e-value: 1.41711E-27
score: 102.761
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 272..336
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 292..329
e-value: 2.0E-5
score: 34.1
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 291..330
e-value: 1.2E-10
score: 41.6
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 292..330
score: 12.415513
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 264..336
e-value: 5.4E-23
score: 82.2

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G19871Cucsat.G19871gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G19871.T4.E1Cucsat.G19871.T4.E1exon
Cucsat.G19871.T4.E2Cucsat.G19871.T4.E2exon
Cucsat.G19871.T4.E3Cucsat.G19871.T4.E3exon
Cucsat.G19871.T4.E4Cucsat.G19871.T4.E4exon
Cucsat.G19871.T4.E5Cucsat.G19871.T4.E5exon
Cucsat.G19871.T4.E6Cucsat.G19871.T4.E6exon
Cucsat.G19871.T4.E7Cucsat.G19871.T4.E7exon
Cucsat.G19871.T4.E8Cucsat.G19871.T4.E8exon
Cucsat.G19871.T4.E9Cucsat.G19871.T4.E9exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G19871.T4.C1Cucsat.G19871.T4.C1CDS
Cucsat.G19871.T4.C2Cucsat.G19871.T4.C2CDS
Cucsat.G19871.T4.C3Cucsat.G19871.T4.C3CDS
Cucsat.G19871.T4.C4Cucsat.G19871.T4.C4CDS
Cucsat.G19871.T4.C5Cucsat.G19871.T4.C5CDS
Cucsat.G19871.T4.C6Cucsat.G19871.T4.C6CDS
Cucsat.G19871.T4.C7Cucsat.G19871.T4.C7CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G19871.T4Cucsat.G19871.T4-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0061630 ubiquitin protein ligase activity