Cucsat.G19511.T27 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G19511.T27
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Heparanase-like protein 3
Location	ctg4: 1465520 .. 1469161 (-)
RNA-Seq Expression	Cucsat.G19511.T27
Synteny	Cucsat.G19511.T27

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TACTATACATCGATCTTTACTTAAAATCATTCAATAAGATATGTGAATTTAAAGATCTAGACGTTTCATGATAATATCATTTGATAGACATTCACGATACTTTGTTTTACGATTTCTCTTTGTTACATCCAACCACGCACCTCAACACTAATAATTTCTTCCATCACCACCTCCCCTAGAAGAGGAAAGGAAAGGAAAACACACAAGGGTTTCTTTTCCTCTATTTCTTTAATGCAGATCCGACATTGAAATTGTTAAATGGTCCATTCATTAGCATCATTTTAACCTACATTTCTTCATTTCTTCATTTCCAAGGCTTGTATTGTATTACCATATATATATATAAACATTTCAAGTATTTCTTTTCATCACTCTTCATATTCTTCCTTTCTTTACACTCTGTCTGCTTCCCTTTATTAATGGCCAGCAAGTAGCTCTGAAATCGCTTGAAGTTTCATGGCTTCTTCCTCTTCTTCTTCTGTCTTTGGTTATTTCGCCATTTGAGAAAGTTTTTAGAAATGGGTTTTCGTTTTTGGCTTCTGCGGTTTTGTTTTTGGGTCTGTTTCTTCTTTCTCTGTTGTGGTTCAGTGAACTCAGAAGGGTTTTTGAGAGGGAGGGTTTTGATTGACGGCAAATCCGCCATTGGACGTATTGATGAAGACTTTGTTTGTGCTACGCTCGATTGGTGGCCTCCTGAGAAATGCGATTACGGAACTTGCAGTTGGGGCCAAGCTTCTCTGTTGAATCTGGTATTTGTTTTTTCTTTTCTTTCTTTTAGATTCTAATCATTTCCAAACTCATTAATGGCGATTTACTTTTCTTATGAATTACGAGAGTTCATAAATTTTGGCATCTGGGTTTCATTATTTGATATTCTTCTTTAAGTTTTCTTTATGGGTTTTTAGAGTATTTATTGCTAAAGAAAAGTGAGAATCCTTTCATCTTTTCGAACAGTCTCACTCCTCAGTTATGCATTTGGAAGATAACAAAACAAAGAAAACTCGACATTAATTGGTTTGGAGGAGATATCAAAACAAAGAAAGTTCGAAGATTAATTTTGAGTATAACTATTGGTTAGAAAATTGAACCTCTAATCTCTATCTAACAGGAGGTCATGTCAATACTACTAAATAGAGTTCATTCCGGCAAAAACTCGAAAATCAATTAAATTTCAAAAGTCTCTTAAATTATACATTTCGTTTACTTAGGTCTCGTTTGGAAAGTAGATAGCAGAAAGAAAAAAAAAAAAACCCCAATTAATTGGATTTTAAATTTGGAAGTATGTTTACAATTATTTAATGTCTTTTCTACTAGGTTCTATTGACTAAAGAATAATAATAGTTATGAGGTCACATCTACTATTAAATATACTTTCCTATAATCTATCTAGTGCTATACTATCTGAAATATTTAAATTTGTTCTGATTTCATTTTCAACTGCAGGACCTGGGCAACAACATTCTATTAAATGCAGTCAAAGGTTTGAATTCCTTTAGCTCCTGGACTGTTTTGGCTTACAGTTTTAATGAATCTAGCTTTATAAAGAAGTAAGAAAGTCTTTATGATCTGCAAAAGAATGAAATAAACCCTAGGCTTATCTAATCTATGATGATTTGTTGAAGCTAATATTTAATATCTATTATAAAAGATAGGTTTCATAGGTTCAATTTATGTGTCCAAACATAGGTTTATATCATACCTCAAGTAATTCAACGTTTTAGGGGAGAAAGATCAAAAAAGTGTTAAGAGTTGTAGAACGGAACCTTAAAGTTTGGTCCTCGGTGAGATTTCATGTTTCTTTTTTATACTTCGATTTTGAGGACTTTTTCTAATTACTCTCTAGGCAACATCATACGTAGTTGAACCTCCTTTTTCTAGTGGGGTTTTTGTGGGCTCTTCTTTCATTTTTTTTTTTTCCTGAATGAAAGAAGTTGTTTCTATTCGGGAAAAAATCATTCTACCTATGAAGTATTCTCTATATATTTGAATGTGAAAGTTAAAACTAATGTCTCTTCATGAATGGCGCAGAATTTAAACCCCTAAAGCTTAGATTGGGTGGTACTTTGCAAGATAAGATCATATATGATACTGAAGATCACCAGGAGCCATGCATTTATCTTTCAAAAAACACCTCAGAGTTATTTGGGTTCAGTCAAGGCTGCTTGCCTACTAACAGATGGGACCAACTAAATGACTTCTTCAAGAAAGGAGGGTAATAAAAAATTCATCCCATTTTCCTCGTATGAAGTTAGATTTCTCAAAAGTTGAATGCTAAAGTTGTGTAATCTTATTCAGAGCTAAGATTATCTTCGGATTAAATGCTCTCAATGGACGACAAATTGCAGCAAATGGGTGTTCTGTTGGAGCTTGGAATTACACAAATGCTGAAATGTTAATTCGTTATACTGTAAAAAAAAACTACACCATCCATGGTTGGGAGCTTGGTAAGAATTGCCAATTGTTTTCCTAATGAACAATTCAAAACCCCGAGATATGTTGGTCTACAACAATGAATTTTGGAGAGTAATTGTTCTTGTTATTTGATTTTTTCTTATGCTCAGGAAATGAATTGAGTGGGAATGGAGTTGGGACAAGAATTTTAGCAGAACAGTATGCTTCTGATACAATTGTTCTTCAAAACATGGTCCAAAGTATCTACAAGGATATCGAACCGAAACCATTAATCATAGCACCTGGAGGATTCTTTGATGAAAATTGGTTCAAAGAATTCGTTGGCAAAACTACCCCATCACTAGACGTTGTCACACACCATATATACAATCTAGGACCAGGTATCATTCATTGGCCTAGTAATACTTACATGAAGTTTCGAAAAGCAGGTAGACATTGAAGCCTTTTCTTATTAACAGGAGTCGATGAGCATCTTGTTGAAAAGATTCTTGATCCGTCCTATCTTGATGGCATGGTTGATACATTTAACAAGCTCCATGAGATCCTCAAAAACTCTCCAACTTCAGCTAAAGCGTGGGTTGGGGAATCAGGAGGGGCTTACAACAGTGGCCATAACCTGGTCACAAATGCATTCGTCTTTAGTTTCTGGTAATTTAGACTGTGAAAGACATTCATATTTGGTTCTTTCAAGACATCCATTACAAATAGAACTCTCTCATTGATGTGTAATTTATTCAGGTACTTGGACCAGCTCGGTATGGCAGCTGCTTATGATACAAAAACATACTGCAGGCAGACTTTGATTGGTGGAAACTACGGCTTACTAAACACGACAACCTTTGAACCGAATCCAGACTATTACAGGTGAAAGATTAGGTCAATATTTCTGACCTACTCCACTGCTATTAGACAATTATCTAACAATCAGCTTGCTTGTTTTTTGTTTCTTTCTTCGTTTCAGTGCACTCTTATGGCATCGCTTGATGGGAAGAAATGTTCTGTCCACGGGCTTTAATGGGACAGAGAAAATACGAGCCTATGCACATTGTTCTAAGCAATCTGTAAGTAGCAGTATATACCATGACAACCTTAAATTTGGTTTTTCTGCCTATTGCTTAAGTAAATGAAATAAACAGCTGAAAGCTAAAACTAGAAATAAAGAAATGCCACCAGTTTCTCAACTCTGATTTAACTCTCAATCTTACAGAAAGGAATCACATTACTATTGCT

Coding sequence (CDS)

ATGTTAATTCGTTATACTGTAAAAAAAAACTACACCATCCATGGTTGGGAGCTTGGAAATGAATTGAGTGGGAATGGAGTTGGGACAAGAATTTTAGCAGAACAGTATGCTTCTGATACAATTGTTCTTCAAAACATGGTCCAAAGTATCTACAAGGATATCGAACCGAAACCATTAATCATAGCACCTGGAGGATTCTTTGATGAAAATTGGTTCAAAGAATTCGTTGGCAAAACTACCCCATCACTAGACGTTGTCACACACCATATATACAATCTAGGACCAGGAGTCGATGAGCATCTTGTTGAAAAGATTCTTGATCCGTCCTATCTTGATGGCATGGTTGATACATTTAACAAGCTCCATGAGATCCTCAAAAACTCTCCAACTTCAGCTAAAGCGTGGGTTGGGGAATCAGGAGGGGCTTACAACAGTGGCCATAACCTGGTCACAAATGCATTCGTCTTTAGTTTCTGGTACTTGGACCAGCTCGGTATGGCAGCTGCTTATGATACAAAAACATACTGCAGGCAGACTTTGATTGGTGGAAACTACGGCTTACTAAACACGACAACCTTTGAACCGAATCCAGACTATTACAGGTGA

Protein sequence

MLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKPLIIAPGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVDTFNKLHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFWYLDQLGMAAAYDTKTYCRQTLIGGNYGLLNTTTFEPNPDYYR

Homology

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BLAST of Cucsat.G19511.T27 vs. ExPASy Swiss-Prot
Match: Q9FZP1 (Heparanase-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=At5g34940 PE=2 SV=2)

HSP 1 Score: 302.8 bits (774), Expect = 3.0e-81
Identity = 136/198 (68.69%), Postives = 160/198 (80.81%), Query Frame = 0


Query: 3   IRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKPLIIA 62
           IR+T + NYTI GWELGNEL G+GVG R+ A QYA DTI L+N+V  +YK++ P PL+I 
Sbjct: 184 IRFTAENNYTIDGWELGNELCGSGVGARVGANQYAIDTINLRNIVNRVYKNVSPMPLVIG 243

Query: 63  PGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVDTFNKLH 122
           PGGFF+ +WF E++ K   SL+  T HIY+LGPGVDEHL+EKIL+PSYLD    +F  L 
Sbjct: 244 PGGFFEVDWFTEYLNKAENSLNATTRHIYDLGPGVDEHLIEKILNPSYLDQEAKSFRSLK 303

Query: 123 EILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFWYLDQLGMAAAYDTKTYCRQTLIG 182
            I+KNS T A AWVGESGGAYNSG NLV+NAFV+SFWYLDQLGMA+ YDTKTYCRQ+LIG
Sbjct: 304 NIIKNSSTKAVAWVGESGGAYNSGRNLVSNAFVYSFWYLDQLGMASLYDTKTYCRQSLIG 363

Query: 183 GNYGLLNTTTFEPNPDYY 201
           GNYGLLNTT F PNPDYY
Sbjct: 364 GNYGLLNTTNFTPNPDYY 381

[Close]

BLAST of Cucsat.G19511.T27 vs. ExPASy Swiss-Prot
Match: Q8L608 (Heparanase-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At5g61250 PE=2 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 1.4e-62
Identity = 106/199 (53.27%), Postives = 142/199 (71.36%), Query Frame = 0


Query: 3   IRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKPLIIA 62
           + YTV K Y I  WE GNELSG+G+   +  E Y  D IVL+N+++++YK+   KPL++A
Sbjct: 180 MNYTVSKGYAIDSWEFGNELSGSGIWASVSVELYGKDLIVLKNVIKNVYKNSRTKPLVVA 239

Query: 63  PGGFFDENWFKEFVGKTTPS-LDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVDTFNKL 122
           PGGFF+E W+ E +  + P  LDV+THHIYNLGPG D  LV KILDP+YL G+ + F  +
Sbjct: 240 PGGFFEEQWYSELLRLSGPGVLDVLTHHIYNLGPGNDPKLVNKILDPNYLSGISELFANV 299

Query: 123 HEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFWYLDQLGMAAAYDTKTYCRQTLI 182
           ++ ++     A AWVGE+GGA+NSG   V+  F+ SFWYLDQLG+++ ++TK YCRQ L+
Sbjct: 300 NQTIQEHGPWAAAWVGEAGGAFNSGGRQVSETFINSFWYLDQLGISSKHNTKVYCRQALV 359

Query: 183 GGNYGLLNTTTFEPNPDYY 201
           GG YGLL   TF PNPDYY
Sbjct: 360 GGFYGLLEKETFVPNPDYY 378

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BLAST of Cucsat.G19511.T27 vs. ExPASy Swiss-Prot
Match: Q9FF10 (Heparanase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g07830 PE=2 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 1.3e-60
Identity = 108/200 (54.00%), Postives = 143/200 (71.50%), Query Frame = 0


Query: 3   IRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDI-EPKPLII 62
           + YTV K Y I  WE GNELSG+GVG  + AE Y  D IVL++++  +YK+    KP+++
Sbjct: 183 LNYTVSKGYVIDSWEFGNELSGSGVGASVSAELYGKDLIVLKDVINKVYKNSWLHKPILV 242

Query: 63  APGGFFDENWFKEFVGKTTPS-LDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVDTFNK 122
           APGGF+++ W+ + +  + PS +DVVTHHIYNLG G D  LV+KI+DPSYL  +  TF  
Sbjct: 243 APGGFYEQQWYTKLLEISGPSVVDVVTHHIYNLGSGNDPALVKKIMDPSYLSQVSKTFKD 302

Query: 123 LHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFWYLDQLGMAAAYDTKTYCRQTL 182
           +++ ++     A  WVGESGGAYNSG   V++ F+ SFWYLDQLGM+A ++TK YCRQTL
Sbjct: 303 VNQTIQEHGPWASPWVGESGGAYNSGGRHVSDTFIDSFWYLDQLGMSARHNTKVYCRQTL 362

Query: 183 IGGNYGLLNTTTFEPNPDYY 201
           +GG YGLL   TF PNPDYY
Sbjct: 363 VGGFYGLLEKGTFVPNPDYY 382

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BLAST of Cucsat.G19511.T27 vs. ExPASy Swiss-Prot
Match: Q9LRC8 (Baicalin-beta-D-glucuronidase OS=Scutellaria baicalensis OX=65409 GN=SGUS PE=1 SV=1)

HSP 1 Score: 208.0 bits (528), Expect = 1.0e-52
Identity = 100/201 (49.75%), Postives = 132/201 (65.67%), Query Frame = 0


Query: 2   LIRYTVKKNYT-IHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKPLI 61
           LI Y++KK Y  I GW LGNEL G+ +   +  E YA+D   L  +V+ IY+D    PLI
Sbjct: 192 LIEYSLKKGYKHIRGWTLGNELGGHTLFIGVSPEDYANDAKKLHELVKEIYQDQGTMPLI 251

Query: 62  IAPGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVDT-FN 121
           IAPG  FD  W+ EF+ + TP L V THH+YNLG G D+ L + +L  S+ D    + + 
Sbjct: 252 IAPGAIFDLEWYTEFIDR-TPELHVATHHMYNLGSGGDDALKDVLLTASFFDEATKSMYE 311

Query: 122 KLHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFWYLDQLGMAAAYDTKTYCRQT 181
            L +I+    T A AW+GE+GGA+NSG + ++N F+  FWYL+ LG +A  DTKT+CRQT
Sbjct: 312 GLQKIVNRPGTKAVAWIGEAGGAFNSGQDGISNTFINGFWYLNMLGYSALLDTKTFCRQT 371

Query: 182 LIGGNYGLLNTTTFEPNPDYY 201
           L GGNYGLL T T+ PNPDYY
Sbjct: 372 LTGGNYGLLQTGTYIPNPDYY 391

[Close]

BLAST of Cucsat.G19511.T27 vs. ExPASy Swiss-Prot
Match: X4Y2L4 (Hyaluronoglucuronidase OS=Hirudo nipponia OX=42736 PE=1 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 3.1e-22
Identity = 65/201 (32.34%), Postives = 96/201 (47.76%), Query Frame = 0


Query: 2   LIRYTVKKNYTIH-GWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKPLI 61
           L +Y V K Y  +  WELGNE   +      L E+   +     + V   Y  +    L+
Sbjct: 156 LFKYCVSKGYGDNIDWELGNE--PDHTSAHNLTEKQVGEDFKALHKVLEKYPTLNKGSLV 215

Query: 62  IAPGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVDTFNK 121
               G+   ++ K         +   T H Y       +  V   LD +Y   +   F+K
Sbjct: 216 GPDVGWMGVSYVKGLADGAGDHVTAFTLHQYYFDGNTSD--VSTYLDATYFKKLQQLFDK 275

Query: 122 LHEILKNSPTSAK-AWVGESGGAYNSGHNLVTNAFVFSFWYLDQLGMAAAYDTKTYCRQT 181
           + ++LKNSP   K  W+GE+   YNSG   V++ +V  F  LD+LG++AA + K   RQT
Sbjct: 276 VKDVLKNSPHKDKPLWLGETSSGYNSGTKDVSDRYVSGFLTLDKLGLSAANNVKVVIRQT 335

Query: 182 LIGGNYGLLNTTTFEPNPDYY 201
           +  G YGLL+  T EPNPDY+
Sbjct: 336 IYNGYYGLLDKNTLEPNPDYW 352

[Close]

BLAST of Cucsat.G19511.T27 vs. NCBI nr
Match: XP_004139281.1 (heparanase-like protein 3 [Cucumis sativus] >KAE8651538.1 hypothetical protein Csa_019474 [Cucumis sativus])

HSP 1 Score: 421 bits (1083), Expect = 6.40e-144
Identity = 200/200 (100.00%), Postives = 200/200 (100.00%), Query Frame = 0


Query: 1   MLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKPLI 60
           MLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKPLI
Sbjct: 183 MLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKPLI 242

Query: 61  IAPGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVDTFNK 120
           IAPGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVDTFNK
Sbjct: 243 IAPGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVDTFNK 302

Query: 121 LHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFWYLDQLGMAAAYDTKTYCRQTL 180
           LHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFWYLDQLGMAAAYDTKTYCRQTL
Sbjct: 303 LHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFWYLDQLGMAAAYDTKTYCRQTL 362

Query: 181 IGGNYGLLNTTTFEPNPDYY 200
           IGGNYGLLNTTTFEPNPDYY
Sbjct: 363 IGGNYGLLNTTTFEPNPDYY 382

[Close]

BLAST of Cucsat.G19511.T27 vs. NCBI nr
Match: XP_008457259.1 (PREDICTED: LOW QUALITY PROTEIN: heparanase-like protein 3 [Cucumis melo])

HSP 1 Score: 415 bits (1066), Expect = 2.33e-141
Identity = 195/200 (97.50%), Postives = 199/200 (99.50%), Query Frame = 0


Query: 1   MLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKPLI 60
           MLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKPLI
Sbjct: 183 MLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKPLI 242

Query: 61  IAPGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVDTFNK 120
           IAPGGFFDENW+KEF+ KTTP+LDVVTHHIYNLGPGVDEHLVE+ILDPSYLDGMVDTFNK
Sbjct: 243 IAPGGFFDENWYKEFIDKTTPTLDVVTHHIYNLGPGVDEHLVERILDPSYLDGMVDTFNK 302

Query: 121 LHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFWYLDQLGMAAAYDTKTYCRQTL 180
           LHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFWYLDQLGMAAAYDTKTYCRQTL
Sbjct: 303 LHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFWYLDQLGMAAAYDTKTYCRQTL 362

Query: 181 IGGNYGLLNTTTFEPNPDYY 200
           IGGNYGLLNTTTFEPNPDYY
Sbjct: 363 IGGNYGLLNTTTFEPNPDYY 382

[Close]

BLAST of Cucsat.G19511.T27 vs. NCBI nr
Match: KAA0063515.1 (heparanase-like protein 3 [Cucumis melo var. makuwa] >TYJ97842.1 heparanase-like protein 3 [Cucumis melo var. makuwa])

HSP 1 Score: 415 bits (1066), Expect = 3.00e-141
Identity = 195/200 (97.50%), Postives = 199/200 (99.50%), Query Frame = 0


Query: 1   MLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKPLI 60
           MLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKPLI
Sbjct: 191 MLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKPLI 250

Query: 61  IAPGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVDTFNK 120
           IAPGGFFDENW+KEF+ KTTP+LDVVTHHIYNLGPGVDEHLVE+ILDPSYLDGMVDTFNK
Sbjct: 251 IAPGGFFDENWYKEFIDKTTPTLDVVTHHIYNLGPGVDEHLVERILDPSYLDGMVDTFNK 310

Query: 121 LHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFWYLDQLGMAAAYDTKTYCRQTL 180
           LHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFWYLDQLGMAAAYDTKTYCRQTL
Sbjct: 311 LHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFWYLDQLGMAAAYDTKTYCRQTL 370

Query: 181 IGGNYGLLNTTTFEPNPDYY 200
           IGGNYGLLNTTTFEPNPDYY
Sbjct: 371 IGGNYGLLNTTTFEPNPDYY 390

[Close]

BLAST of Cucsat.G19511.T27 vs. NCBI nr
Match: XP_038891041.1 (heparanase-like protein 3 isoform X2 [Benincasa hispida])

HSP 1 Score: 399 bits (1026), Expect = 1.48e-137
Identity = 187/200 (93.50%), Postives = 195/200 (97.50%), Query Frame = 0


Query: 1   MLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKPLI 60
           MLIRYTVKKNYTIHGWELGNELSGNGVGTRI AEQYASDTI LQNMVQSIYK IEPKPLI
Sbjct: 23  MLIRYTVKKNYTIHGWELGNELSGNGVGTRISAEQYASDTIALQNMVQSIYKGIEPKPLI 82

Query: 61  IAPGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVDTFNK 120
           IAPGGFFDENWFKEF+GKTT SLDVVTHHIYNLGPGVDEHL+E+ILDPSYLDGMV+TFNK
Sbjct: 83  IAPGGFFDENWFKEFIGKTTQSLDVVTHHIYNLGPGVDEHLLERILDPSYLDGMVNTFNK 142

Query: 121 LHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFWYLDQLGMAAAYDTKTYCRQTL 180
           LHEIL++SPTSAKAWVGE+GGAYNSGHNLVTNAFVFSFWYLDQLGMAA +DTKTYCRQTL
Sbjct: 143 LHEILEDSPTSAKAWVGEAGGAYNSGHNLVTNAFVFSFWYLDQLGMAATFDTKTYCRQTL 202

Query: 181 IGGNYGLLNTTTFEPNPDYY 200
           IGGNYGLLNTTTFEPNPDYY
Sbjct: 203 IGGNYGLLNTTTFEPNPDYY 222

[Close]

BLAST of Cucsat.G19511.T27 vs. NCBI nr
Match: XP_038891040.1 (heparanase-like protein 3 isoform X1 [Benincasa hispida])

HSP 1 Score: 399 bits (1026), Expect = 2.75e-135
Identity = 187/200 (93.50%), Postives = 195/200 (97.50%), Query Frame = 0


Query: 1   MLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKPLI 60
           MLIRYTVKKNYTIHGWELGNELSGNGVGTRI AEQYASDTI LQNMVQSIYK IEPKPLI
Sbjct: 183 MLIRYTVKKNYTIHGWELGNELSGNGVGTRISAEQYASDTIALQNMVQSIYKGIEPKPLI 242

Query: 61  IAPGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVDTFNK 120
           IAPGGFFDENWFKEF+GKTT SLDVVTHHIYNLGPGVDEHL+E+ILDPSYLDGMV+TFNK
Sbjct: 243 IAPGGFFDENWFKEFIGKTTQSLDVVTHHIYNLGPGVDEHLLERILDPSYLDGMVNTFNK 302

Query: 121 LHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFWYLDQLGMAAAYDTKTYCRQTL 180
           LHEIL++SPTSAKAWVGE+GGAYNSGHNLVTNAFVFSFWYLDQLGMAA +DTKTYCRQTL
Sbjct: 303 LHEILEDSPTSAKAWVGEAGGAYNSGHNLVTNAFVFSFWYLDQLGMAATFDTKTYCRQTL 362

Query: 181 IGGNYGLLNTTTFEPNPDYY 200
           IGGNYGLLNTTTFEPNPDYY
Sbjct: 363 IGGNYGLLNTTTFEPNPDYY 382

[Close]

BLAST of Cucsat.G19511.T27 vs. ExPASy TrEMBL
Match: A0A1S3C565 (LOW QUALITY PROTEIN: heparanase-like protein 3 OS=Cucumis melo OX=3656 GN=LOC103496979 PE=3 SV=1)

HSP 1 Score: 415 bits (1066), Expect = 1.13e-141
Identity = 195/200 (97.50%), Postives = 199/200 (99.50%), Query Frame = 0


Query: 1   MLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKPLI 60
           MLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKPLI
Sbjct: 183 MLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKPLI 242

Query: 61  IAPGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVDTFNK 120
           IAPGGFFDENW+KEF+ KTTP+LDVVTHHIYNLGPGVDEHLVE+ILDPSYLDGMVDTFNK
Sbjct: 243 IAPGGFFDENWYKEFIDKTTPTLDVVTHHIYNLGPGVDEHLVERILDPSYLDGMVDTFNK 302

Query: 121 LHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFWYLDQLGMAAAYDTKTYCRQTL 180
           LHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFWYLDQLGMAAAYDTKTYCRQTL
Sbjct: 303 LHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFWYLDQLGMAAAYDTKTYCRQTL 362

Query: 181 IGGNYGLLNTTTFEPNPDYY 200
           IGGNYGLLNTTTFEPNPDYY
Sbjct: 363 IGGNYGLLNTTTFEPNPDYY 382

[Close]

BLAST of Cucsat.G19511.T27 vs. ExPASy TrEMBL
Match: A0A5A7VC89 (Heparanase-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G00540 PE=3 SV=1)

HSP 1 Score: 415 bits (1066), Expect = 1.45e-141
Identity = 195/200 (97.50%), Postives = 199/200 (99.50%), Query Frame = 0


Query: 1   MLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKPLI 60
           MLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKPLI
Sbjct: 191 MLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKPLI 250

Query: 61  IAPGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVDTFNK 120
           IAPGGFFDENW+KEF+ KTTP+LDVVTHHIYNLGPGVDEHLVE+ILDPSYLDGMVDTFNK
Sbjct: 251 IAPGGFFDENWYKEFIDKTTPTLDVVTHHIYNLGPGVDEHLVERILDPSYLDGMVDTFNK 310

Query: 121 LHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFWYLDQLGMAAAYDTKTYCRQTL 180
           LHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFWYLDQLGMAAAYDTKTYCRQTL
Sbjct: 311 LHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFWYLDQLGMAAAYDTKTYCRQTL 370

Query: 181 IGGNYGLLNTTTFEPNPDYY 200
           IGGNYGLLNTTTFEPNPDYY
Sbjct: 371 IGGNYGLLNTTTFEPNPDYY 390

[Close]

BLAST of Cucsat.G19511.T27 vs. ExPASy TrEMBL
Match: A0A6J1FD96 (heparanase-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111444334 PE=3 SV=1)

HSP 1 Score: 392 bits (1007), Expect = 1.24e-132
Identity = 182/198 (91.92%), Postives = 191/198 (96.46%), Query Frame = 0


Query: 3   IRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKPLIIA 62
           +RYTV+KNYTIHGWELGNELSGNGVGTR+ AEQYASDTI LQNMVQSIYKDIE KPLII+
Sbjct: 190 VRYTVRKNYTIHGWELGNELSGNGVGTRVTAEQYASDTIALQNMVQSIYKDIEYKPLIIS 249

Query: 63  PGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVDTFNKLH 122
           PGGFFDENWFKEF+ KTT SLDVVTHHIYNLGPGVDEHLVE+ILDPSYLDGM DTF KLH
Sbjct: 250 PGGFFDENWFKEFIDKTTQSLDVVTHHIYNLGPGVDEHLVERILDPSYLDGMADTFYKLH 309

Query: 123 EILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFWYLDQLGMAAAYDTKTYCRQTLIG 182
           EIL+NSPTSAKAWVGE+GGAYNSGHNLVTNAFVFSFWYLDQLGM+AAYDTKTYCRQTLIG
Sbjct: 310 EILRNSPTSAKAWVGEAGGAYNSGHNLVTNAFVFSFWYLDQLGMSAAYDTKTYCRQTLIG 369

Query: 183 GNYGLLNTTTFEPNPDYY 200
           GNYGLLNTTTFEPNPDYY
Sbjct: 370 GNYGLLNTTTFEPNPDYY 387

[Close]

BLAST of Cucsat.G19511.T27 vs. ExPASy TrEMBL
Match: A0A6J1HPC5 (heparanase-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111465495 PE=3 SV=1)

HSP 1 Score: 389 bits (999), Expect = 1.89e-131
Identity = 182/198 (91.92%), Postives = 190/198 (95.96%), Query Frame = 0


Query: 3   IRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKPLIIA 62
           IRYTVKKNYTIHGWELGNELSGNGVGTR+ AEQYASDTI LQNMVQSIYKDIE KPLII+
Sbjct: 190 IRYTVKKNYTIHGWELGNELSGNGVGTRVTAEQYASDTIALQNMVQSIYKDIEYKPLIIS 249

Query: 63  PGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVDTFNKLH 122
           PGGFFDENWFKEF+ KTT SLDVVTHHIYNLG GVDEHLVE+ILDPSYLDGM DTF KLH
Sbjct: 250 PGGFFDENWFKEFIDKTTQSLDVVTHHIYNLGSGVDEHLVERILDPSYLDGMADTFYKLH 309

Query: 123 EILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFWYLDQLGMAAAYDTKTYCRQTLIG 182
           EIL+NSPTSAKAWVGE+GGAYNSGHNLVTNAFVFSFWYLDQLGM+AA+DTKTYCRQTLIG
Sbjct: 310 EILRNSPTSAKAWVGEAGGAYNSGHNLVTNAFVFSFWYLDQLGMSAAFDTKTYCRQTLIG 369

Query: 183 GNYGLLNTTTFEPNPDYY 200
           GNYGLLNTTTFEPNPDYY
Sbjct: 370 GNYGLLNTTTFEPNPDYY 387

[Close]

BLAST of Cucsat.G19511.T27 vs. ExPASy TrEMBL
Match: A0A6J1CML0 (heparanase-like protein 3 OS=Momordica charantia OX=3673 GN=LOC111013018 PE=3 SV=1)

HSP 1 Score: 380 bits (976), Expect = 4.61e-128
Identity = 176/198 (88.89%), Postives = 188/198 (94.95%), Query Frame = 0


Query: 3   IRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKPLIIA 62
           IRYTVKKNYTIHGWELGNELSGNGVGTRI A+QYASDT  LQNMV+SIY+ IEPKPLIIA
Sbjct: 183 IRYTVKKNYTIHGWELGNELSGNGVGTRISADQYASDTYALQNMVRSIYQGIEPKPLIIA 242

Query: 63  PGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVDTFNKLH 122
           PGGFFDENWFK+F+ KT  S DV+THHIYNLGPGVDEHLVE+ILDPSYLDGMVDTF +LH
Sbjct: 243 PGGFFDENWFKDFIDKTXQSADVITHHIYNLGPGVDEHLVERILDPSYLDGMVDTFYRLH 302

Query: 123 EILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFWYLDQLGMAAAYDTKTYCRQTLIG 182
           EILK+SPTSAK WVGE+GGAYNSGHNLVTNAFVFSFWYLDQLGM+AA+DTKTYCRQTLIG
Sbjct: 303 EILKSSPTSAKPWVGEAGGAYNSGHNLVTNAFVFSFWYLDQLGMSAAFDTKTYCRQTLIG 362

Query: 183 GNYGLLNTTTFEPNPDYY 200
           GNYGLLNTTTFEPNPDYY
Sbjct: 363 GNYGLLNTTTFEPNPDYY 380

The following BLAST results are available for this feature:

BLAST of Cucsat.G19511.T27 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FZP1	3.0e-81	68.69	Heparanase-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=At5g34940 PE=2 SV=2	[more]
Q8L608	1.4e-62	53.27	Heparanase-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At5g61250 PE=2 SV=1	[more]
Q9FF10	1.3e-60	54.00	Heparanase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g07830 PE=2 SV=1	[more]
Q9LRC8	1.0e-52	49.75	Baicalin-beta-D-glucuronidase OS=Scutellaria baicalensis OX=65409 GN=SGUS PE=1 S...	[more]
X4Y2L4	3.1e-22	32.34	Hyaluronoglucuronidase OS=Hirudo nipponia OX=42736 PE=1 SV=1	[more]

BLAST of Cucsat.G19511.T27 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004139281.1	6.40e-144	100.00	heparanase-like protein 3 [Cucumis sativus] >KAE8651538.1 hypothetical protein C...	[more]
XP_008457259.1	2.33e-141	97.50	PREDICTED: LOW QUALITY PROTEIN: heparanase-like protein 3 [Cucumis melo]	[more]
KAA0063515.1	3.00e-141	97.50	heparanase-like protein 3 [Cucumis melo var. makuwa] >TYJ97842.1 heparanase-like...	[more]
XP_038891041.1	1.48e-137	93.50	heparanase-like protein 3 isoform X2 [Benincasa hispida]	[more]
XP_038891040.1	2.75e-135	93.50	heparanase-like protein 3 isoform X1 [Benincasa hispida]	[more]

BLAST of Cucsat.G19511.T27 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C565	1.13e-141	97.50	LOW QUALITY PROTEIN: heparanase-like protein 3 OS=Cucumis melo OX=3656 GN=LOC103...	[more]
A0A5A7VC89	1.45e-141	97.50	Heparanase-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A6J1FD96	1.24e-132	91.92	heparanase-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111444334 PE=3 SV=...	[more]
A0A6J1HPC5	1.89e-131	91.92	heparanase-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111465495 PE=3 SV=1	[more]
A0A6J1CML0	4.61e-128	88.89	heparanase-like protein 3 OS=Momordica charantia OX=3673 GN=LOC111013018 PE=3 SV...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 1..201 e-value: 8.0E-45 score: 155.6
None	No IPR available	PANTHER	PTHR14363:SF34	HEPARANASE-LIKE PROTEIN 3	coord: 2..200
None	No IPR available	PANTHER	PTHR14363	HEPARANASE-RELATED	coord: 2..200
IPR005199	Glycoside hydrolase, family 79	PFAM	PF03662	Glyco_hydro_79n	coord: 2..165 e-value: 3.6E-77 score: 259.4
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 2..200

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G19511	Cucsat.G19511	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G19511.T27.E1	Cucsat.G19511.T27.E1	exon
Cucsat.G19511.T27.E2	Cucsat.G19511.T27.E2	exon
Cucsat.G19511.T27.E3	Cucsat.G19511.T27.E3	exon
Cucsat.G19511.T27.E4	Cucsat.G19511.T27.E4	exon
Cucsat.G19511.T27.E5	Cucsat.G19511.T27.E5	exon
Cucsat.G19511.T27.E6	Cucsat.G19511.T27.E6	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G19511.T27.C4	Cucsat.G19511.T27.C4	CDS
Cucsat.G19511.T27.C3	Cucsat.G19511.T27.C3	CDS
Cucsat.G19511.T27.C2	Cucsat.G19511.T27.C2	CDS
Cucsat.G19511.T27.C1	Cucsat.G19511.T27.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G19511.T27	Cucsat.G19511.T27-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016798	hydrolase activity, acting on glycosyl bonds