Cucsat.G19511.T19 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G19511.T19
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionHeparanase-like protein 3
Locationctg4: 1463786 .. 1469150 (-)
RNA-Seq ExpressionCucsat.G19511.T19
SyntenyCucsat.G19511.T19
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GATCTTTACTTAAAATCATTCAATAAGATATGTGAATTTAAAGATCTAGACGTTTCATGATAATATCATTTGATAGACATTCACGATACTTTGTTTTACGATTTCTCTTTGTTACATCCAACCACGCACCTCAACACTAATAATTTCTTCCATCACCACCTCCCCTAGAAGAGGAAAGGAAAGGAAAACACACAAGGGTTTCTTTTCCTCTATTTCTTTAATGCAGATCCGACATTGAAATTGTTAAATGGTCCATTCATTAGCATCATTTTAACCTACATTTCTTCATTTCTTCATTTCCAAGGCTTGTATTGTATTACCATATATATATATAAACATTTCAAGTATTTCTTTTCATCACTCTTCATATTCTTCCTTTCTTTACACTCTGTCTGCTTCCCTTTATTAATGGCCAGCAAGTAGCTCTGAAATCGCTTGAAGTTTCATGGCTTCTTCCTCTTCTTCTTCTGTCTTTGGTTATTTCGCCATTTGAGAAAGTTTTTAGAAATGGGTTTTCGTTTTTGGCTTCTGCGGTTTTGTTTTTGGGTCTGTTTCTTCTTTCTCTGTTGTGGTTCAGTGAACTCAGAAGGGTTTTTGAGAGGGAGGGTTTTGATTGACGGCAAATCCGCCATTGGACGTATTGATGAAGACTTTGTTTGTGCTACGCTCGATTGGTGGCCTCCTGAGAAATGCGATTACGGAACTTGCAGTTGGGGCCAAGCTTCTCTGTTGAATCTGGTATTTGTTTTTTCTTTTCTTTCTTTTAGATTCTAATCATTTCCAAACTCATTAATGGCGATTTACTTTTCTTATGAATTACGAGAGTTCATAAATTTTGGCATCTGGGTTTCATTATTTGATATTCTTCTTTAAGTTTTCTTTATGGGTTTTTAGAGTATTTATTGCTAAAGAAAAGTGAGAATCCTTTCATCTTTTCGAACAGTCTCACTCCTCAGTTATGCATTTGGAAGATAACAAAACAAAGAAAACTCGACATTAATTGGTTTGGAGGAGATATCAAAACAAAGAAAGTTCGAAGATTAATTTTGAGTATAACTATTGGTTAGAAAATTGAACCTCTAATCTCTATCTAACAGGAGGTCATGTCAATACTACTAAATAGAGTTCATTCCGGCAAAAACTCGAAAATCAATTAAATTTCAAAAGTCTCTTAAATTATACATTTCGTTTACTTAGGTCTCGTTTGGAAAGTAGATAGCAGAAAGAAAAAAAAAAAAACCCCAATTAATTGGATTTTAAATTTGGAAGTATGTTTACAATTATTTAATGTCTTTTCTACTAGGTTCTATTGACTAAAGAATAATAATAGTTATGAGGTCACATCTACTATTAAATATACTTTCCTATAATCTATCTAGTGCTATACTATCTGAAATATTTAAATTTGTTCTGATTTCATTTTCAACTGCAGGACCTGGGCAACAACATTCTATTAAATGCAGTCAAAGGTTTGAATTCCTTTAGCTCCTGGACTGTTTTGGCTTACAGTTTTAATGAATCTAGCTTTATAAAGAAGTAAGAAAGTCTTTATGATCTGCAAAAGAATGAAATAAACCCTAGGCTTATCTAATCTATGATGATTTGTTGAAGCTAATATTTAATATCTATTATAAAAGATAGGTTTCATAGGTTCAATTTATGTGTCCAAACATAGGTTTATATCATACCTCAAGTAATTCAACGTTTTAGGGGAGAAAGATCAAAAAAGTGTTAAGAGTTGTAGAACGGAACCTTAAAGTTTGGTCCTCGGTGAGATTTCATGTTTCTTTTTTATACTTCGATTTTGAGGACTTTTTCTAATTACTCTCTAGGCAACATCATACGTAGTTGAACCTCCTTTTTCTAGTGGGGTTTTTGTGGGCTCTTCTTTCATTTTTTTTTTTTCCTGAATGAAAGAAGTTGTTTCTATTCGGGAAAAAATCATTCTACCTATGAAGTATTCTCTATATATTTGAATGTGAAAGTTAAAACTAATGTCTCTTCATGAATGGCGCAGAATTTAAACCCCTAAAGCTTAGATTGGGTGGTACTTTGCAAGATAAGATCATATATGATACTGAAGATCACCAGGAGCCATGCATTTATCTTTCAAAAAACACCTCAGAGTTATTTGGGTTCAGTCAAGGCTGCTTGCCTACTAACAGATGGGACCAACTAAATGACTTCTTCAAGAAAGGAGGGTAATAAAAAATTCATCCCATTTTCCTCGTATGAAGTTAGATTTCTCAAAAGTTGAATGCTAAAGTTGTGTAATCTTATTCAGAGCTAAGATTATCTTCGGATTAAATGCTCTCAATGGACGACAAATTGCAGCAAATGGGTGTTCTGTTGGAGCTTGGAATTACACAAATGCTGAAATGTTAATTCGTTATACTGTAAAAAAAAACTACACCATCCATGGTTGGGAGCTTGGTAAGAATTGCCAATTGTTTTCCTAATGAACAATTCAAAACCCCGAGATATGTTGGTCTACAACAATGAATTTTGGAGAGTAATTGTTCTTGTTATTTGATTTTTTCTTATGCTCAGGAAATGAATTGAGTGGGAATGGAGTTGGGACAAGAATTTTAGCAGAACAGTATGCTTCTGATACAATTGTTCTTCAAAACATGGTCCAAAGTATCTACAAGGATATCGAACCGAAACCATTAATCATAGCACCTGGAGGATTCTTTGATGAAAATTGGTTCAAAGAATTCGTTGGCAAAACTACCCCATCACTAGACGTTGTCACACACCATATATACAATCTAGGACCAGGTATCATTCATTGGCCTAGTAATACTTACATGAAGTTTCGAAAAGCAGGTAGACATTGAAGCCTTTTCTTATTAACAGGAGTCGATGAGCATCTTGTTGAAAAGATTCTTGATCCGTCCTATCTTGATGGCATGGTTGATACATTTAACAAGCTCCATGAGATCCTCAAAAACTCTCCAACTTCAGCTAAAGCGTGGGTTGGGGAATCAGGAGGGGCTTACAACAGTGGCCATAACCTGGTCACAAATGCATTCGTCTTTAGTTTCTGGTAATTTAGACTGTGAAAGACATTCATATTTGGTTCTTTCAAGACATCCATTACAAATAGAACTCTCTCATTGATGTGTAATTTATTCAGGTACTTGGACCAGCTCGGTATGGCAGCTGCTTATGATACAAAAACATACTGCAGGCAGACTTTGATTGGTGGAAACTACGGCTTACTAAACACGACAACCTTTGAACCGAATCCAGACTATTACAGGTGAAAGATTAGGTCAATATTTCTGACCTACTCCACTGCTATTAGACAATTATCTAACAATCAGCTTGCTTGTTTTTTGTTTCTTTCTTCGTTTCAGTGCACTCTTATGGCATCGCTTGATGGGAAGAAATGTTCTGTCCACGGGCTTTAATGGGACAGAGAAAATACGAGCCTATGCACATTGTTCTAAGCAATCTGTAAGTAGCAGTATATACCATGACAACCTTAAATTTGGTTTTTCTGCCTATTGCTTAAGTAAATGAAATAAACAGCTGAAAGCTAAAACTAGAAATAAAGAAATGCCACCAGTTTCTCAACTCTGATTTAACTCTCAATCTTACAGAAAGGAATCACATTACTATTGCTCAACCTAGATGGTAACAATACAGTTCATGCTGGGATTGCATTCAACAGAACTACAGCGTTGCACCATAAACACAGATCCAATAAGCATAAATCAAATATTGTTCGGATACCTCGACCTCGTGGTCTTGAAGGCGAAGCCTTCAGAGAGGAATATCATTTAACAGCCAAGGATGGAAACCTGCATAGTCAAACAGTGCTGCTAAATGGAAACATTTTGTCTGTAAATTCATCAGGGAACATACCTCCATTGGAACCTCAGCATGTAAATTCATCTGAACCAATAATGGTGGCTCCCTTTTCAATTGTATTCATTCACATACCAAATATCATTCTCCCTGCTTGCAGATAGGATAGGTTGAATGTATGGAACTGAAAATCGGTTCCAGATTGGTTATTTTCTAATAAGAGAAAACAATTGTAACATTTGGTTCATTTTTTTCAAGGTGAAAAAATAGCAAGAGTCTCTTTGAATCAACATTGACCGACGGAAAATCAATCCGTGGATGTAAGTAGCACCATAAGCAGAAGATGCAAATTTTACAGAATATATGCAACTTTCGCAGGATCACTCTGACTAACAATGAGAAAACAAAATCTCTACATGATCAGTAATACTGCTTCTTTGTGGGGAATTATACATGTATTCACTACAGATATACACAACTGAAGAACCTGATATGAAATGCAGTTGATGGATCGTATTGGAGTTCTCAGCAAATTCGTGTAATCTAACACTAATCATTCTTTGAAAAGTTTTTAGGTTTACTGCTGGCGAAGACCAACCAAACAATTGTCCCAGTTAAGTAGAAGAAGATTGATGGGGCAAACAATGAGATCTACACATAGAAAACAGGAAATTTTGAAAATGGGAAAGAAGTCAGAGGATATCAATGAAATATATCAAATGTTAAAGAAAAGGAGAAAATTGTCAACTTACACTCCAAGAATGAGTTGAATCGAGAAGAAAGCCAGTGAGAGCAACTCCTACAATGCCAGGTATTGCTCCAACGGTATTTGTAATGCCCTGAGGAGAATTGGCGAAGCTTGCTAAAATAAACCACATGAAATATTAAGAATATTGAAATGAACAGGGGTATTATAGAGCTTCAGCTTACATACCAAAAGAACACTTGCATATTCAGGTGAAATATCTTGATGAGTACAGTAGAGTCCTGCAGAAAACAGATAAAGATTTTGGTACCTTAAAGGAAATAGGAGACTTTGGTCCAATAACTTCGAAAAAGAATGTTTCAAGAATTGAAGTATCTTTGATGTAGATTTTACCTGATAATGCAAAACTAGAGAGGGCCAAGCCACTGGTTAGAATTCCTACTACCTCCCAAGGTGGCAATCCTAAATCAACAGAGGACAAAGTCATGCAAACTGCTGGGGACACAAAGGCAACTGTTTGGCAAATCTTTCTTACCTACATAAGGAAAACAGAAGAGGATTAAACAAATTATGAATGGAATGCTTCTTGAAAACAAACTGTTGGAGATCGTCTTTCCATACTTTTGTGGTATCGACACCATTGGAAATCAGGTTATCAGCTAACTGTGATGCAATGCTTGTCACAAAAATTGAAGCCAGGGGTGGCAGGATGGAAACCTGAATTGAATTATTTCTAGGAGTCATAAGAAAGTTGACGCATTACACAACCACACGGTTGCAATCAGGTAATAAAATAATGACGCGAAAATGAAGAAAAATGAGAATAGAAAAAGAGAGGTACCC

Coding sequence (CDS)

ATGGGTTTTCGTTTTTGGCTTCTGCGGTTTTGTTTTTGGGTCTGTTTCTTCTTTCTCTGTTGTGGTTCAGTGAACTCAGAAGGGTTTTTGAGAGGGAGGGTTTTGATTGACGGCAAATCCGCCATTGGACGTATTGATGAAGACTTTGTTTGTGCTACGCTCGATTGGTGGCCTCCTGAGAAATGCGATTACGGAACTTGCAGTTGGGGCCAAGCTTCTCTGTTGAATCTGGACCTGGGCAACAACATTCTATTAAATGCAGTCAAAGAATTTAAACCCCTAAAGCTTAGATTGGGTGGTACTTTGCAAGATAAGATCATATATGATACTGAAGATCACCAGGAGCCATGCATTTATCTTTCAAAAAACACCTCAGAGTTATTTGGGTTCAGTCAAGGCTGCTTGCCTACTAACAGATGGGACCAACTAAATGACTTCTTCAAGAAAGGAGGAGCTAAGATTATCTTCGGATTAAATGCTCTCAATGGACGACAAATTGCAGCAAATGGGTGTTCTGTTGGAGCTTGGAATTACACAAATGCTGAAATGTTAATTCGTTATACTGTAAAAAAAAACTACACCATCCATGGTTGGGAGCTTGGAAATGAATTGAGTGGGAATGGAGTTGGGACAAGAATTTTAGCAGAACAGTATGCTTCTGATACAATTGTTCTTCAAAACATGGTCCAAAGTATCTACAAGGATATCGAACCGAAACCATTAATCATAGCACCTGGAGGATTCTTTGATGAAAATTGGTTCAAAGAATTCGTTGGCAAAACTACCCCATCACTAGACGTTGTCACACACCATATATACAATCTAGGACCAGGAGTCGATGAGCATCTTGTTGAAAAGATTCTTGATCCGTCCTATCTTGATGGCATGGTTGATACATTTAACAAGCTCCATGAGATCCTCAAAAACTCTCCAACTTCAGCTAAAGCGTGGGTTGGGGAATCAGGAGGGGCTTACAACAGTGGCCATAACCTGGTCACAAATGCATTCGTCTTTAGTTTCTGGTAA

Protein sequence

MGFRFWLLRFCFWVCFFFLCCGSVNSEGFLRGRVLIDGKSAIGRIDEDFVCATLDWWPPEKCDYGTCSWGQASLLNLDLGNNILLNAVKEFKPLKLRLGGTLQDKIIYDTEDHQEPCIYLSKNTSELFGFSQGCLPTNRWDQLNDFFKKGGAKIIFGLNALNGRQIAANGCSVGAWNYTNAEMLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKPLIIAPGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVDTFNKLHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFW
Homology
BLAST of Cucsat.G19511.T19 vs. ExPASy Swiss-Prot
Match: Q9FZP1 (Heparanase-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=At5g34940 PE=2 SV=2)

HSP 1 Score: 457.6 bits (1176), Expect = 1.2e-127
Identity = 210/341 (61.58%), Postives = 261/341 (76.54%), Query Frame = 0

Query: 1   MGFRFWLLRFCFWVCFFFLCCGSVNSEGFLRGRVLIDGKSAIGRIDEDFVCATLDWWPPE 60
           M +R  L    F   F FL C +V+S     G V + G++A+G IDEDF+CATLDWWPPE
Sbjct: 1   MAYRQILAIVLFLCVFQFLDC-TVSSAVEENGTVFVYGRAAVGTIDEDFICATLDWWPPE 60

Query: 61  KCDYGTCSWGQASLLNLDLGNNILLNAVKEFKPLKLRLGGTLQDKIIYDTEDHQEPCIYL 120
           KCDYG+CSW  AS+LNLDL N IL NA+K F PLK+R+GGTLQD +IY+T D ++PC+  
Sbjct: 61  KCDYGSCSWDHASILNLDLNNVILQNAIKAFAPLKIRIGGTLQDIVIYETPDSKQPCLPF 120

Query: 121 SKNTSELFGFSQGCLPTNRWDQLNDFFKKGGAKIIFGLNALNGRQIAANGCSVGAWNYTN 180
           +KN+S LFG++QGCLP  RWD+LN FF+K G K+IFGLNAL+GR I +NG ++GAWNYTN
Sbjct: 121 TKNSSILFGYTQGCLPMRRWDELNAFFRKTGTKVIFGLNALSGRSIKSNGEAIGAWNYTN 180

Query: 181 AEMLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKP 240
           AE  IR+T + NYTI GWELGNEL G+GVG R+ A QYA DTI L+N+V  +YK++ P P
Sbjct: 181 AESFIRFTAENNYTIDGWELGNELCGSGVGARVGANQYAIDTINLRNIVNRVYKNVSPMP 240

Query: 241 LIIAPGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVDTF 300
           L+I PGGFF+ +WF E++ K   SL+  T HIY+LGPGVDEHL+EKIL+PSYLD    +F
Sbjct: 241 LVIGPGGFFEVDWFTEYLNKAENSLNATTRHIYDLGPGVDEHLIEKILNPSYLDQEAKSF 300

Query: 301 NKLHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFW 342
             L  I+KNS T A AWVGESGGAYNSG NLV+NAFV+SFW
Sbjct: 301 RSLKNIIKNSSTKAVAWVGESGGAYNSGRNLVSNAFVYSFW 340

BLAST of Cucsat.G19511.T19 vs. ExPASy Swiss-Prot
Match: Q8L608 (Heparanase-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At5g61250 PE=2 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 9.5e-96
Identity = 154/312 (49.36%), Postives = 211/312 (67.63%), Query Frame = 0

Query: 31  RGRVLIDGKSAIGRIDEDFVCATLDWWPPEKCDYGTCSWGQASLLNLDLGNNILLNAVKE 90
           R  ++IDG   I   DE+F+CATLDWWPPEKC+Y  C WG ASL+NL+L + +L  A++ 
Sbjct: 26  RTTLVIDGSRRIAETDENFICATLDWWPPEKCNYDQCPWGYASLINLNLASPLLAKAIQA 85

Query: 91  FKPLKLRLGGTLQDKIIYDTEDHQEPCIYLSKNTSELFGFSQGCLPTNRWDQLNDFFKKG 150
           F+ L++R+GG+LQD++IYD  D + PC    K    LFGFS+GCL   RWD++N FF   
Sbjct: 86  FRTLRIRIGGSLQDQVIYDVGDLKTPCTQFKKTDDGLFGFSEGCLYMKRWDEVNHFFNAT 145

Query: 151 GAKIIFGLNALNGRQIAANGCSVGAWNYTNAEMLIRYTVKKNYTIHGWELGNELSGNGVG 210
           GA + FGLNAL+GR         G W++TN +  + YTV K Y I  WE GNELSG+G+ 
Sbjct: 146 GAIVTFGLNALHGRNKLNGTAWGGDWDHTNTQDFMNYTVSKGYAIDSWEFGNELSGSGIW 205

Query: 211 TRILAEQYASDTIVLQNMVQSIYKDIEPKPLIIAPGGFFDENWFKEFVGKTTPS-LDVVT 270
             +  E Y  D IVL+N+++++YK+   KPL++APGGFF+E W+ E +  + P  LDV+T
Sbjct: 206 ASVSVELYGKDLIVLKNVIKNVYKNSRTKPLVVAPGGFFEEQWYSELLRLSGPGVLDVLT 265

Query: 271 HHIYNLGPGVDEHLVEKILDPSYLDGMVDTFNKLHEILKNSPTSAKAWVGESGGAYNSGH 330
           HHIYNLGPG D  LV KILDP+YL G+ + F  +++ ++     A AWVGE+GGA+NSG 
Sbjct: 266 HHIYNLGPGNDPKLVNKILDPNYLSGISELFANVNQTIQEHGPWAAAWVGEAGGAFNSGG 325

Query: 331 NLVTNAFVFSFW 342
             V+  F+ SFW
Sbjct: 326 RQVSETFINSFW 337

BLAST of Cucsat.G19511.T19 vs. ExPASy Swiss-Prot
Match: Q9FF10 (Heparanase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g07830 PE=2 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 3.0e-89
Identity = 158/343 (46.06%), Postives = 219/343 (63.85%), Query Frame = 0

Query: 1   MGFRFWLLRFCFWVCFFFLCCGSVNSEGFLRGRVLIDGKSAIGRIDEDFVCATLDWWPPE 60
           MGFR  ++   F  C   L      ++   R  ++I G   +   DE+FVCATLDWWP +
Sbjct: 1   MGFRVCVI-VVFLGC-LLLVPEKTMAQEMKRASIVIQGARRVCETDENFVCATLDWWPHD 60

Query: 61  KCDYGTCSWGQASLLNLDLGNNILLNAVKEFKPLKLRLGGTLQDKIIYDTEDHQEPCIYL 120
           KC+Y  C WG +S++N+DL   +L  A+K FKPL++R+GG+LQD++IYD  + + PC   
Sbjct: 61  KCNYDQCPWGYSSVINMDLTRPLLTKAIKAFKPLRIRIGGSLQDQVIYDVGNLKTPCRPF 120

Query: 121 SKNTSELFGFSQGCLPTNRWDQLNDFFKKGGAKIIFGLNALNGRQIAANGCSVGAWNYTN 180
            K  S LFGFS+GCL   RWD+LN F    GA + FGLNAL GR         GAW++ N
Sbjct: 121 QKMNSGLFGFSKGCLHMKRWDELNSFLTATGAVVTFGLNALRGRHKLRGKAWGGAWDHIN 180

Query: 181 AEMLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDI-EPK 240
            +  + YTV K Y I  WE GNELSG+GVG  + AE Y  D IVL++++  +YK+    K
Sbjct: 181 TQDFLNYTVSKGYVIDSWEFGNELSGSGVGASVSAELYGKDLIVLKDVINKVYKNSWLHK 240

Query: 241 PLIIAPGGFFDENWFKEFVGKTTPS-LDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVD 300
           P+++APGGF+++ W+ + +  + PS +DVVTHHIYNLG G D  LV+KI+DPSYL  +  
Sbjct: 241 PILVAPGGFYEQQWYTKLLEISGPSVVDVVTHHIYNLGSGNDPALVKKIMDPSYLSQVSK 300

Query: 301 TFNKLHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFW 342
           TF  +++ ++     A  WVGESGGAYNSG   V++ F+ SFW
Sbjct: 301 TFKDVNQTIQEHGPWASPWVGESGGAYNSGGRHVSDTFIDSFW 341

BLAST of Cucsat.G19511.T19 vs. ExPASy Swiss-Prot
Match: Q9LRC8 (Baicalin-beta-D-glucuronidase OS=Scutellaria baicalensis OX=65409 GN=SGUS PE=1 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 3.2e-83
Identity = 155/356 (43.54%), Postives = 216/356 (60.67%), Query Frame = 0

Query: 1   MGFRFWLLRFCFWVCFFFLCCGSVNSEGFLRGRVLIDGKSAIGRIDEDFVCATLDWWPPE 60
           MGF+ W    C         CG +  E  +   V I+ ++ + + DE++VCATLD WPP 
Sbjct: 1   MGFQVWQKGLCVLCFSLIFICGVIGEETTI---VKIE-ENPVAQTDENYVCATLDLWPPT 60

Query: 61  KCDYGTCSWGQASLLNLDLGNNILLNAVKEFKPLKLRLGGTLQDKIIYDTEDHQEPC-IY 120
           KC+YG C WG++S LNLDL NNI+ NAVKEF PLKLR GGTLQD+++Y T    EPC   
Sbjct: 61  KCNYGNCPWGKSSFLNLDLNNNIIRNAVKEFAPLKLRFGGTLQDRLVYQT-SRDEPCDST 120

Query: 121 LSKNTSELFGFSQGCLPTNRWDQLNDFFKKGGAKIIFGLNALNGRQIAANG--------- 180
              NT+ +  FS  CL  +RWD++N F  + G++ +FGLNAL G+ +   G         
Sbjct: 121 FYNNTNLILDFSHACLSLDRWDEINQFILETGSEAVFGLNALRGKTVEIKGIIKDGQYLG 180

Query: 181 ---CSVGAWNYTNAEMLIRYTVKKNYT-IHGWELGNELSGNGVGTRILAEQYASDTIVLQ 240
               +VG W+Y+N++ LI Y++KK Y  I GW LGNEL G+ +   +  E YA+D   L 
Sbjct: 181 ETTTAVGEWDYSNSKFLIEYSLKKGYKHIRGWTLGNELGGHTLFIGVSPEDYANDAKKLH 240

Query: 241 NMVQSIYKDIEPKPLIIAPGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEK 300
            +V+ IY+D    PLIIAPG  FD  W+ EF+ + TP L V THH+YNLG G D+ L + 
Sbjct: 241 ELVKEIYQDQGTMPLIIAPGAIFDLEWYTEFIDR-TPELHVATHHMYNLGSGGDDALKDV 300

Query: 301 ILDPSYLDGMVDT-FNKLHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFW 342
           +L  S+ D    + +  L +I+    T A AW+GE+GGA+NSG + ++N F+  FW
Sbjct: 301 LLTASFFDEATKSMYEGLQKIVNRPGTKAVAWIGEAGGAFNSGQDGISNTFINGFW 350

BLAST of Cucsat.G19511.T19 vs. ExPASy Swiss-Prot
Match: X4Y2L4 (Hyaluronoglucuronidase OS=Hirudo nipponia OX=42736 PE=1 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.7e-15
Identity = 80/322 (24.84%), Postives = 130/322 (40.37%), Query Frame = 0

Query: 34  VLIDGKSAIGRIDEDF--VCATLDWWPPEKCDYGTCSWGQASLLNLDLGNNILLNAVKEF 93
           V ID K+ I  + E F  V      + P+    G  S+       +D+ +  L   ++  
Sbjct: 6   VTIDDKNVIASVSESFHGVAFDASLFSPK----GLWSF-------VDITSPKLFKLLEGL 65

Query: 94  KPLKLRLGGTLQDKIIYDTEDHQEPCIY--LSKNTSELFGFS-------QGCLPTNRWDQ 153
            P   R+GGT  + + +D +++ +   Y      T E    +       Q  L    +D 
Sbjct: 66  SPGYFRVGGTFANWLFFDLDENNKWKDYWAFKDKTPETATITRRWLFRKQNNLKKETFDD 125

Query: 154 LNDFFKKGGAKIIFGLNA--LNGRQIAANGCSVGAWNYTNAEMLIRYTVKKNYTIH-GWE 213
           L    K    +++F LNA    G +I     S   W+ + AE L +Y V K Y  +  WE
Sbjct: 126 LVKLTKGSKMRLLFDLNAEVRTGYEIGKKMTS--TWDSSEAEKLFKYCVSKGYGDNIDWE 185

Query: 214 LGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKPLIIAPGGFFDENWFKEFVG 273
           LGNE   +      L E+   +     + V   Y  +    L+    G+   ++ K    
Sbjct: 186 LGNE--PDHTSAHNLTEKQVGEDFKALHKVLEKYPTLNKGSLVGPDVGWMGVSYVKGLAD 245

Query: 274 KTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVDTFNKLHEILKNSPTSAK-AWV 333
                +   T H Y       +  V   LD +Y   +   F+K+ ++LKNSP   K  W+
Sbjct: 246 GAGDHVTAFTLHQYYFDGNTSD--VSTYLDATYFKKLQQLFDKVKDVLKNSPHKDKPLWL 305

Query: 334 GESGGAYNSGHNLVTNAFVFSF 341
           GE+   YNSG   V++ +V  F
Sbjct: 306 GETSSGYNSGTKDVSDRYVSGF 310

BLAST of Cucsat.G19511.T19 vs. NCBI nr
Match: XP_004139281.1 (heparanase-like protein 3 [Cucumis sativus] >KAE8651538.1 hypothetical protein Csa_019474 [Cucumis sativus])

HSP 1 Score: 721 bits (1860), Expect = 1.59e-259
Identity = 341/341 (100.00%), Postives = 341/341 (100.00%), Query Frame = 0

Query: 1   MGFRFWLLRFCFWVCFFFLCCGSVNSEGFLRGRVLIDGKSAIGRIDEDFVCATLDWWPPE 60
           MGFRFWLLRFCFWVCFFFLCCGSVNSEGFLRGRVLIDGKSAIGRIDEDFVCATLDWWPPE
Sbjct: 1   MGFRFWLLRFCFWVCFFFLCCGSVNSEGFLRGRVLIDGKSAIGRIDEDFVCATLDWWPPE 60

Query: 61  KCDYGTCSWGQASLLNLDLGNNILLNAVKEFKPLKLRLGGTLQDKIIYDTEDHQEPCIYL 120
           KCDYGTCSWGQASLLNLDLGNNILLNAVKEFKPLKLRLGGTLQDKIIYDTEDHQEPCIYL
Sbjct: 61  KCDYGTCSWGQASLLNLDLGNNILLNAVKEFKPLKLRLGGTLQDKIIYDTEDHQEPCIYL 120

Query: 121 SKNTSELFGFSQGCLPTNRWDQLNDFFKKGGAKIIFGLNALNGRQIAANGCSVGAWNYTN 180
           SKNTSELFGFSQGCLPTNRWDQLNDFFKKGGAKIIFGLNALNGRQIAANGCSVGAWNYTN
Sbjct: 121 SKNTSELFGFSQGCLPTNRWDQLNDFFKKGGAKIIFGLNALNGRQIAANGCSVGAWNYTN 180

Query: 181 AEMLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKP 240
           AEMLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKP
Sbjct: 181 AEMLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKP 240

Query: 241 LIIAPGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVDTF 300
           LIIAPGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVDTF
Sbjct: 241 LIIAPGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVDTF 300

Query: 301 NKLHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFW 341
           NKLHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFW
Sbjct: 301 NKLHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFW 341

BLAST of Cucsat.G19511.T19 vs. NCBI nr
Match: XP_008457259.1 (PREDICTED: LOW QUALITY PROTEIN: heparanase-like protein 3 [Cucumis melo])

HSP 1 Score: 700 bits (1807), Expect = 1.79e-251
Identity = 326/341 (95.60%), Postives = 336/341 (98.53%), Query Frame = 0

Query: 1   MGFRFWLLRFCFWVCFFFLCCGSVNSEGFLRGRVLIDGKSAIGRIDEDFVCATLDWWPPE 60
           MGFRFWLL FCFWVCFFFLCCGSVNSEGFLRGRV IDGKSAIGRIDEDFVCATLDWWPPE
Sbjct: 1   MGFRFWLLGFCFWVCFFFLCCGSVNSEGFLRGRVFIDGKSAIGRIDEDFVCATLDWWPPE 60

Query: 61  KCDYGTCSWGQASLLNLDLGNNILLNAVKEFKPLKLRLGGTLQDKIIYDTEDHQEPCIYL 120
           KCDYGTCSWGQASLLNLDLGNNILLNAVKEFKPLKLRLGGTLQDK+IYDTEDH+EPCIY 
Sbjct: 61  KCDYGTCSWGQASLLNLDLGNNILLNAVKEFKPLKLRLGGTLQDKLIYDTEDHREPCIYF 120

Query: 121 SKNTSELFGFSQGCLPTNRWDQLNDFFKKGGAKIIFGLNALNGRQIAANGCSVGAWNYTN 180
           S+N SELFGF+QGCLPTNRWDQLNDFFKKGGAKIIFGLNALNGR+IAANGCS+GAWNYTN
Sbjct: 121 SRNASELFGFTQGCLPTNRWDQLNDFFKKGGAKIIFGLNALNGREIAANGCSIGAWNYTN 180

Query: 181 AEMLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKP 240
           AEMLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKP
Sbjct: 181 AEMLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKP 240

Query: 241 LIIAPGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVDTF 300
           LIIAPGGFFDENW+KEF+ KTTP+LDVVTHHIYNLGPGVDEHLVE+ILDPSYLDGMVDTF
Sbjct: 241 LIIAPGGFFDENWYKEFIDKTTPTLDVVTHHIYNLGPGVDEHLVERILDPSYLDGMVDTF 300

Query: 301 NKLHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFW 341
           NKLHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFW
Sbjct: 301 NKLHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFW 341

BLAST of Cucsat.G19511.T19 vs. NCBI nr
Match: XP_038891040.1 (heparanase-like protein 3 isoform X1 [Benincasa hispida])

HSP 1 Score: 661 bits (1705), Expect = 6.18e-236
Identity = 312/341 (91.50%), Postives = 326/341 (95.60%), Query Frame = 0

Query: 1   MGFRFWLLRFCFWVCFFFLCCGSVNSEGFLRGRVLIDGKSAIGRIDEDFVCATLDWWPPE 60
           MGFR WLL FCFWVCFFFL  GSVNSEGFLRGRVLIDGKSAIG IDEDFVCATLDWWPPE
Sbjct: 1   MGFRVWLLGFCFWVCFFFLSSGSVNSEGFLRGRVLIDGKSAIGGIDEDFVCATLDWWPPE 60

Query: 61  KCDYGTCSWGQASLLNLDLGNNILLNAVKEFKPLKLRLGGTLQDKIIYDTEDHQEPCIYL 120
           KCDYGTCSWGQASLLNLDLGNNILLNAVKEFKPLK+RLGG+LQDKIIYDTEDHQ+PCI  
Sbjct: 61  KCDYGTCSWGQASLLNLDLGNNILLNAVKEFKPLKIRLGGSLQDKIIYDTEDHQQPCISF 120

Query: 121 SKNTSELFGFSQGCLPTNRWDQLNDFFKKGGAKIIFGLNALNGRQIAANGCSVGAWNYTN 180
           S+N SELFGFSQGCLPTNRWDQLNDFFKKGG KIIFGLNALNGRQIAA+G +VGAWN +N
Sbjct: 121 SRNASELFGFSQGCLPTNRWDQLNDFFKKGGTKIIFGLNALNGRQIAADGSAVGAWNCSN 180

Query: 181 AEMLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKP 240
           AEMLIRYTVKKNYTIHGWELGNELSGNGVGTRI AEQYASDTI LQNMVQSIYK IEPKP
Sbjct: 181 AEMLIRYTVKKNYTIHGWELGNELSGNGVGTRISAEQYASDTIALQNMVQSIYKGIEPKP 240

Query: 241 LIIAPGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVDTF 300
           LIIAPGGFFDENWFKEF+GKTT SLDVVTHHIYNLGPGVDEHL+E+ILDPSYLDGMV+TF
Sbjct: 241 LIIAPGGFFDENWFKEFIGKTTQSLDVVTHHIYNLGPGVDEHLLERILDPSYLDGMVNTF 300

Query: 301 NKLHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFW 341
           NKLHEIL++SPTSAKAWVGE+GGAYNSGHNLVTNAFVFSFW
Sbjct: 301 NKLHEILEDSPTSAKAWVGEAGGAYNSGHNLVTNAFVFSFW 341

BLAST of Cucsat.G19511.T19 vs. NCBI nr
Match: KAA0063515.1 (heparanase-like protein 3 [Cucumis melo var. makuwa] >TYJ97842.1 heparanase-like protein 3 [Cucumis melo var. makuwa])

HSP 1 Score: 640 bits (1651), Expect = 1.30e-227
Identity = 306/342 (89.47%), Postives = 315/342 (92.11%), Query Frame = 0

Query: 24  VNSEGFLRGRVLIDGKSAIGRIDEDFVCATLDWWPPEKCDYGTCSWGQASLLNL------ 83
           VNSEGFLRGRVLIDGKSAIGRIDEDFVCATLDWWPPEKCDYGTCSWGQASLLNL      
Sbjct: 8   VNSEGFLRGRVLIDGKSAIGRIDEDFVCATLDWWPPEKCDYGTCSWGQASLLNLVFLFFF 67

Query: 84  ------------------DLGNNILLNAVKEFKPLKLRLGGTLQDKIIYDTEDHQEPCIY 143
                             DLGNNILLNAVKEFKPLKLRLGGTLQDK+IYDTEDH+EPCIY
Sbjct: 68  FISFLSSFRFQSFPNSFMDLGNNILLNAVKEFKPLKLRLGGTLQDKLIYDTEDHREPCIY 127

Query: 144 LSKNTSELFGFSQGCLPTNRWDQLNDFFKKGGAKIIFGLNALNGRQIAANGCSVGAWNYT 203
            S+N SELFGF+QGCLPTNRWDQLNDFFKKGGAKIIFGLNALNGR+IAANGCSVGAWNYT
Sbjct: 128 FSRNASELFGFTQGCLPTNRWDQLNDFFKKGGAKIIFGLNALNGREIAANGCSVGAWNYT 187

Query: 204 NAEMLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPK 263
           NAEMLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPK
Sbjct: 188 NAEMLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPK 247

Query: 264 PLIIAPGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVDT 323
           PLIIAPGGFFDENW+KEF+ KTTP+LDVVTHHIYNLGPGVDEHLVE+ILDPSYLDGMVDT
Sbjct: 248 PLIIAPGGFFDENWYKEFIDKTTPTLDVVTHHIYNLGPGVDEHLVERILDPSYLDGMVDT 307

Query: 324 FNKLHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFW 341
           FNKLHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFW
Sbjct: 308 FNKLHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFW 349

BLAST of Cucsat.G19511.T19 vs. NCBI nr
Match: XP_023537510.1 (heparanase-like protein 3 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023537511.1 heparanase-like protein 3 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 634 bits (1634), Expect = 5.00e-225
Identity = 298/348 (85.63%), Postives = 322/348 (92.53%), Query Frame = 0

Query: 1   MGFRFWLLRFCFWVCFFFLCCGSVNS-------EGFLRGRVLIDGKSAIGRIDEDFVCAT 60
           MGFR WL+ FCFWVC  FL CGSVNS       +GF+RGRVLIDGKSAIG IDEDFVCAT
Sbjct: 1   MGFRVWLVGFCFWVC--FLVCGSVNSKGLGLEEDGFVRGRVLIDGKSAIGGIDEDFVCAT 60

Query: 61  LDWWPPEKCDYGTCSWGQASLLNLDLGNNILLNAVKEFKPLKLRLGGTLQDKIIYDTEDH 120
           LDWWPPEKCDYGTCSWG+ASLLNLDLGNN+LLNAVKEFKPLKLRLGGTLQDKIIY+TEDH
Sbjct: 61  LDWWPPEKCDYGTCSWGRASLLNLDLGNNVLLNAVKEFKPLKLRLGGTLQDKIIYETEDH 120

Query: 121 QEPCIYLSKNTSELFGFSQGCLPTNRWDQLNDFFKKGGAKIIFGLNALNGRQIAANGCSV 180
           Q+ C+YLS+NTSELFG+SQGCLPT RWD+LN+FFKKGG K+IFGLNALNGRQIA++G +V
Sbjct: 121 QQSCVYLSRNTSELFGYSQGCLPTKRWDELNEFFKKGGVKVIFGLNALNGRQIASDGSAV 180

Query: 181 GAWNYTNAEMLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIY 240
           GAW++TNAE  IRYTVKKNYTIHGWELGNELSGNGVGTR+ AEQYASDTI LQNMVQSIY
Sbjct: 181 GAWDHTNAESFIRYTVKKNYTIHGWELGNELSGNGVGTRVTAEQYASDTIALQNMVQSIY 240

Query: 241 KDIEPKPLIIAPGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYL 300
           KDIE KPLII+PGGFFDENWFKEF+ KTT SLDVVTHHIYNLGPGVDEHLVE+ILDPSYL
Sbjct: 241 KDIEYKPLIISPGGFFDENWFKEFIDKTTQSLDVVTHHIYNLGPGVDEHLVERILDPSYL 300

Query: 301 DGMVDTFNKLHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFW 341
           DGM DTF KLHEIL+NSPTSAKAWVGE+GGAYNSGHNLVTNAFVFSFW
Sbjct: 301 DGMADTFYKLHEILRNSPTSAKAWVGEAGGAYNSGHNLVTNAFVFSFW 346

BLAST of Cucsat.G19511.T19 vs. ExPASy TrEMBL
Match: A0A1S3C565 (LOW QUALITY PROTEIN: heparanase-like protein 3 OS=Cucumis melo OX=3656 GN=LOC103496979 PE=3 SV=1)

HSP 1 Score: 700 bits (1807), Expect = 8.67e-252
Identity = 326/341 (95.60%), Postives = 336/341 (98.53%), Query Frame = 0

Query: 1   MGFRFWLLRFCFWVCFFFLCCGSVNSEGFLRGRVLIDGKSAIGRIDEDFVCATLDWWPPE 60
           MGFRFWLL FCFWVCFFFLCCGSVNSEGFLRGRV IDGKSAIGRIDEDFVCATLDWWPPE
Sbjct: 1   MGFRFWLLGFCFWVCFFFLCCGSVNSEGFLRGRVFIDGKSAIGRIDEDFVCATLDWWPPE 60

Query: 61  KCDYGTCSWGQASLLNLDLGNNILLNAVKEFKPLKLRLGGTLQDKIIYDTEDHQEPCIYL 120
           KCDYGTCSWGQASLLNLDLGNNILLNAVKEFKPLKLRLGGTLQDK+IYDTEDH+EPCIY 
Sbjct: 61  KCDYGTCSWGQASLLNLDLGNNILLNAVKEFKPLKLRLGGTLQDKLIYDTEDHREPCIYF 120

Query: 121 SKNTSELFGFSQGCLPTNRWDQLNDFFKKGGAKIIFGLNALNGRQIAANGCSVGAWNYTN 180
           S+N SELFGF+QGCLPTNRWDQLNDFFKKGGAKIIFGLNALNGR+IAANGCS+GAWNYTN
Sbjct: 121 SRNASELFGFTQGCLPTNRWDQLNDFFKKGGAKIIFGLNALNGREIAANGCSIGAWNYTN 180

Query: 181 AEMLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKP 240
           AEMLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKP
Sbjct: 181 AEMLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKP 240

Query: 241 LIIAPGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVDTF 300
           LIIAPGGFFDENW+KEF+ KTTP+LDVVTHHIYNLGPGVDEHLVE+ILDPSYLDGMVDTF
Sbjct: 241 LIIAPGGFFDENWYKEFIDKTTPTLDVVTHHIYNLGPGVDEHLVERILDPSYLDGMVDTF 300

Query: 301 NKLHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFW 341
           NKLHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFW
Sbjct: 301 NKLHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFW 341

BLAST of Cucsat.G19511.T19 vs. ExPASy TrEMBL
Match: A0A5A7VC89 (Heparanase-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G00540 PE=3 SV=1)

HSP 1 Score: 640 bits (1651), Expect = 6.29e-228
Identity = 306/342 (89.47%), Postives = 315/342 (92.11%), Query Frame = 0

Query: 24  VNSEGFLRGRVLIDGKSAIGRIDEDFVCATLDWWPPEKCDYGTCSWGQASLLNL------ 83
           VNSEGFLRGRVLIDGKSAIGRIDEDFVCATLDWWPPEKCDYGTCSWGQASLLNL      
Sbjct: 8   VNSEGFLRGRVLIDGKSAIGRIDEDFVCATLDWWPPEKCDYGTCSWGQASLLNLVFLFFF 67

Query: 84  ------------------DLGNNILLNAVKEFKPLKLRLGGTLQDKIIYDTEDHQEPCIY 143
                             DLGNNILLNAVKEFKPLKLRLGGTLQDK+IYDTEDH+EPCIY
Sbjct: 68  FISFLSSFRFQSFPNSFMDLGNNILLNAVKEFKPLKLRLGGTLQDKLIYDTEDHREPCIY 127

Query: 144 LSKNTSELFGFSQGCLPTNRWDQLNDFFKKGGAKIIFGLNALNGRQIAANGCSVGAWNYT 203
            S+N SELFGF+QGCLPTNRWDQLNDFFKKGGAKIIFGLNALNGR+IAANGCSVGAWNYT
Sbjct: 128 FSRNASELFGFTQGCLPTNRWDQLNDFFKKGGAKIIFGLNALNGREIAANGCSVGAWNYT 187

Query: 204 NAEMLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPK 263
           NAEMLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPK
Sbjct: 188 NAEMLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPK 247

Query: 264 PLIIAPGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVDT 323
           PLIIAPGGFFDENW+KEF+ KTTP+LDVVTHHIYNLGPGVDEHLVE+ILDPSYLDGMVDT
Sbjct: 248 PLIIAPGGFFDENWYKEFIDKTTPTLDVVTHHIYNLGPGVDEHLVERILDPSYLDGMVDT 307

Query: 324 FNKLHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFW 341
           FNKLHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFW
Sbjct: 308 FNKLHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFW 349

BLAST of Cucsat.G19511.T19 vs. ExPASy TrEMBL
Match: A0A6J1HPC5 (heparanase-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111465495 PE=3 SV=1)

HSP 1 Score: 630 bits (1624), Expect = 7.49e-224
Identity = 296/348 (85.06%), Postives = 321/348 (92.24%), Query Frame = 0

Query: 1   MGFRFWLLRFCFWVCFFFLCCGSVNS-------EGFLRGRVLIDGKSAIGRIDEDFVCAT 60
           MGFR WL+ FCFWVCF F  CGSVNS       +GF+RGRVLIDGKSAIG IDEDFVCAT
Sbjct: 1   MGFRVWLVGFCFWVCFLF--CGSVNSKGLGLEEDGFVRGRVLIDGKSAIGGIDEDFVCAT 60

Query: 61  LDWWPPEKCDYGTCSWGQASLLNLDLGNNILLNAVKEFKPLKLRLGGTLQDKIIYDTEDH 120
           LDWWPPEKCDYGTCSWG+ASLLNLDLGNN+LLNAVKEFKPLKLRLGGTLQDKIIY+TEDH
Sbjct: 61  LDWWPPEKCDYGTCSWGRASLLNLDLGNNVLLNAVKEFKPLKLRLGGTLQDKIIYETEDH 120

Query: 121 QEPCIYLSKNTSELFGFSQGCLPTNRWDQLNDFFKKGGAKIIFGLNALNGRQIAANGCSV 180
           Q+ C+YLS+NT+ELFG+SQGCLPT RWD+LN+FFKKGG K+IFGLNALNGRQIA++G +V
Sbjct: 121 QQSCVYLSRNTTELFGYSQGCLPTKRWDELNEFFKKGGVKVIFGLNALNGRQIASDGSAV 180

Query: 181 GAWNYTNAEMLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIY 240
           GAW++TNAE  IRYTVKKNYTIHGWELGNELSGNGVGTR+ AEQYASDTI LQNMVQSIY
Sbjct: 181 GAWDHTNAESFIRYTVKKNYTIHGWELGNELSGNGVGTRVTAEQYASDTIALQNMVQSIY 240

Query: 241 KDIEPKPLIIAPGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYL 300
           KDIE KPLII+PGGFFDENWFKEF+ KTT SLDVVTHHIYNLG GVDEHLVE+ILDPSYL
Sbjct: 241 KDIEYKPLIISPGGFFDENWFKEFIDKTTQSLDVVTHHIYNLGSGVDEHLVERILDPSYL 300

Query: 301 DGMVDTFNKLHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFW 341
           DGM DTF KLHEIL+NSPTSAKAWVGE+GGAYNSGHNLVTNAFVFSFW
Sbjct: 301 DGMADTFYKLHEILRNSPTSAKAWVGEAGGAYNSGHNLVTNAFVFSFW 346

BLAST of Cucsat.G19511.T19 vs. ExPASy TrEMBL
Match: A0A6J1FD96 (heparanase-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111444334 PE=3 SV=1)

HSP 1 Score: 625 bits (1613), Expect = 3.78e-222
Identity = 293/348 (84.20%), Postives = 319/348 (91.67%), Query Frame = 0

Query: 1   MGFRFWLLRFCFWVCFFFLCCGSVNS-------EGFLRGRVLIDGKSAIGRIDEDFVCAT 60
           MGFR WL+ FCFWVCF F  CGSVNS       +GF+RGRVLIDGKSAIG IDEDFVCAT
Sbjct: 1   MGFRVWLVGFCFWVCFLF--CGSVNSKGLGLEEDGFVRGRVLIDGKSAIGGIDEDFVCAT 60

Query: 61  LDWWPPEKCDYGTCSWGQASLLNLDLGNNILLNAVKEFKPLKLRLGGTLQDKIIYDTEDH 120
           LDWWPPEKCDYGTCSWG+ASLLNLDLGNN+LLNAVKEFKPLKLRLGGTLQDKIIY+TEDH
Sbjct: 61  LDWWPPEKCDYGTCSWGRASLLNLDLGNNVLLNAVKEFKPLKLRLGGTLQDKIIYETEDH 120

Query: 121 QEPCIYLSKNTSELFGFSQGCLPTNRWDQLNDFFKKGGAKIIFGLNALNGRQIAANGCSV 180
           Q+ C+YLS+NT+ELFG+SQGCL   RWD+LN+FFKKGG K+IFGLNALNGRQIA +G +V
Sbjct: 121 QQSCVYLSRNTTELFGYSQGCLSAKRWDELNEFFKKGGVKVIFGLNALNGRQIALDGSAV 180

Query: 181 GAWNYTNAEMLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIY 240
           GAW++TNAE  +RYTV+KNYTIHGWELGNELSGNGVGTR+ AEQYASDTI LQNMVQSIY
Sbjct: 181 GAWDHTNAESFVRYTVRKNYTIHGWELGNELSGNGVGTRVTAEQYASDTIALQNMVQSIY 240

Query: 241 KDIEPKPLIIAPGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYL 300
           KDIE KPLII+PGGFFDENWFKEF+ KTT SLDVVTHHIYNLGPGVDEHLVE+ILDPSYL
Sbjct: 241 KDIEYKPLIISPGGFFDENWFKEFIDKTTQSLDVVTHHIYNLGPGVDEHLVERILDPSYL 300

Query: 301 DGMVDTFNKLHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFW 341
           DGM DTF KLHEIL+NSPTSAKAWVGE+GGAYNSGHNLVTNAFVFSFW
Sbjct: 301 DGMADTFYKLHEILRNSPTSAKAWVGEAGGAYNSGHNLVTNAFVFSFW 346

BLAST of Cucsat.G19511.T19 vs. ExPASy TrEMBL
Match: A0A6J1CML0 (heparanase-like protein 3 OS=Momordica charantia OX=3673 GN=LOC111013018 PE=3 SV=1)

HSP 1 Score: 602 bits (1552), Expect = 5.18e-213
Identity = 285/340 (83.82%), Postives = 309/340 (90.88%), Query Frame = 0

Query: 8   LRFCFWVCFFFLCCGSVNSEGF------LRGRVLIDGKSAIGRIDEDFVCATLDWWPPEK 67
           + FCFWVCFFF C  SV+S+G       +RGRVLIDGKSAI  IDEDFVCATLDWWPPEK
Sbjct: 1   MGFCFWVCFFF-CFASVSSKGVEEDDGCVRGRVLIDGKSAIAGIDEDFVCATLDWWPPEK 60

Query: 68  CDYGTCSWGQASLLNLDLGNNILLNAVKEFKPLKLRLGGTLQDKIIYDTEDHQEPCIYLS 127
           CDYGTCSWG+ASLLNLDL NNILLNAVKEFKPLKLRLGG+LQDK+IYDTEDHQ+PCI  S
Sbjct: 61  CDYGTCSWGRASLLNLDLDNNILLNAVKEFKPLKLRLGGSLQDKLIYDTEDHQQPCISFS 120

Query: 128 KNTSELFGFSQGCLPTNRWDQLNDFFKKGGAKIIFGLNALNGRQIAANGCSVGAWNYTNA 187
           +NTSELFGFSQGCLPT RWDQLNDFFKKGGAKIIFGLNAL GR+IA++G ++GAWNYTNA
Sbjct: 121 RNTSELFGFSQGCLPTYRWDQLNDFFKKGGAKIIFGLNALTGRRIASDGSAIGAWNYTNA 180

Query: 188 EMLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQSIYKDIEPKPL 247
           E  IRYTVKKNYTIHGWELGNELSGNGVGTRI A+QYASDT  LQNMV+SIY+ IEPKPL
Sbjct: 181 ESFIRYTVKKNYTIHGWELGNELSGNGVGTRISADQYASDTYALQNMVRSIYQGIEPKPL 240

Query: 248 IIAPGGFFDENWFKEFVGKTTPSLDVVTHHIYNLGPGVDEHLVEKILDPSYLDGMVDTFN 307
           IIAPGGFFDENWFK+F+ KT  S DV+THHIYNLGPGVDEHLVE+ILDPSYLDGMVDTF 
Sbjct: 241 IIAPGGFFDENWFKDFIDKTXQSADVITHHIYNLGPGVDEHLVERILDPSYLDGMVDTFY 300

Query: 308 KLHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFW 341
           +LHEILK+SPTSAK WVGE+GGAYNSGHNLVTNAFVFSFW
Sbjct: 301 RLHEILKSSPTSAKPWVGEAGGAYNSGHNLVTNAFVFSFW 339

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FZP11.2e-12761.58Heparanase-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=At5g34940 PE=2 SV=2[more]
Q8L6089.5e-9649.36Heparanase-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At5g61250 PE=2 SV=1[more]
Q9FF103.0e-8946.06Heparanase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g07830 PE=2 SV=1[more]
Q9LRC83.2e-8343.54Baicalin-beta-D-glucuronidase OS=Scutellaria baicalensis OX=65409 GN=SGUS PE=1 S... [more]
X4Y2L41.7e-1524.84Hyaluronoglucuronidase OS=Hirudo nipponia OX=42736 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_004139281.11.59e-259100.00heparanase-like protein 3 [Cucumis sativus] >KAE8651538.1 hypothetical protein C... [more]
XP_008457259.11.79e-25195.60PREDICTED: LOW QUALITY PROTEIN: heparanase-like protein 3 [Cucumis melo][more]
XP_038891040.16.18e-23691.50heparanase-like protein 3 isoform X1 [Benincasa hispida][more]
KAA0063515.11.30e-22789.47heparanase-like protein 3 [Cucumis melo var. makuwa] >TYJ97842.1 heparanase-like... [more]
XP_023537510.15.00e-22585.63heparanase-like protein 3 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023537511.... [more]
Match NameE-valueIdentityDescription
A0A1S3C5658.67e-25295.60LOW QUALITY PROTEIN: heparanase-like protein 3 OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A5A7VC896.29e-22889.47Heparanase-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A6J1HPC57.49e-22485.06heparanase-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111465495 PE=3 SV=1[more]
A0A6J1FD963.78e-22284.20heparanase-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111444334 PE=3 SV=... [more]
A0A6J1CML05.18e-21383.82heparanase-like protein 3 OS=Momordica charantia OX=3673 GN=LOC111013018 PE=3 SV... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005199Glycoside hydrolase, family 79PFAMPF03662Glyco_hydro_79ncoord: 32..341
e-value: 2.3E-149
score: 496.6
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 63..341
e-value: 7.0E-56
score: 191.9
NoneNo IPR availablePANTHERPTHR14363:SF34HEPARANASE-LIKE PROTEIN 3coord: 11..341
NoneNo IPR availablePANTHERPTHR14363HEPARANASE-RELATEDcoord: 11..341
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 45..341

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G19511Cucsat.G19511gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G19511.T19.E1Cucsat.G19511.T19.E1exon
Cucsat.G19511.T19.E2Cucsat.G19511.T19.E2exon
Cucsat.G19511.T19.E3Cucsat.G19511.T19.E3exon
Cucsat.G19511.T19.E4Cucsat.G19511.T19.E4exon
Cucsat.G19511.T19.E5Cucsat.G19511.T19.E5exon
Cucsat.G19511.T19.E6Cucsat.G19511.T19.E6exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G19511.T19.C6Cucsat.G19511.T19.C6CDS
Cucsat.G19511.T19.C5Cucsat.G19511.T19.C5CDS
Cucsat.G19511.T19.C4Cucsat.G19511.T19.C4CDS
Cucsat.G19511.T19.C3Cucsat.G19511.T19.C3CDS
Cucsat.G19511.T19.C2Cucsat.G19511.T19.C2CDS
Cucsat.G19511.T19.C1Cucsat.G19511.T19.C1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G19511.T19Cucsat.G19511.T19-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016798 hydrolase activity, acting on glycosyl bonds