Cucsat.G19362.T1 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G19362.T1
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionLong-chain-alcohol oxidase
Locationctg384: 7360 .. 10846 (+)
RNA-Seq ExpressionCucsat.G19362.T1
SyntenyCucsat.G19362.T1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGATAAAAAATCAATTAGTTCCTAAAGAAGAAATTTGTTGGACCATTTGTTTATTTCCAATTTGGCAAACATAATCTTTGCTACGTGTTTCAAATTGGATAAAGATTGGGACTCGATACTTTTTTTGGATATCAAATCCCTTTCTCTCTCTCTCTCTCTCTCCCCTTTGTTTATTTATTGCGGTATTAATCACACTTCTTTGCCGGAGCTCAGTCTAACCCCACCGGAGAGGAAATCTCAAGCTCAACAAGAACCATTAATGGAGGATACAGAAGTTCCATCTCACCCTTTATTGAGAACACGAAGAACAGACCTCAACCATGACTTCTCTTCTTCTCAAATGGAAGCCATGGCTGCCTTCTGTGAAGCCCTCATTCCCCCTGTGCCTCTTTTTAAAGAAAGCCCACTTGATCAATCTCTTCTTTCATTCTACCAATCCTCTGCTTTTCAAGCTCCAATCCCAGATGAGGTTATATTTTTACTTTTAATTCCTTTTAAGCTTTTATTCTTTCTTGTACTGAGAACTTGTTTGTTTGTTTGTTCATATGGATTTTTAGGTAGCTGACCTATTGGTGGAGAGGGCTAATCCAAAAGCTGTGTTTCTTGTGAAATTGGTTCTGAGACTTCTCTCTTTCAGAATTGGAACTTTGTTGCTTTGCGGGAATGTTTGTTTTGATTGGAGATGGCCTTTCTTTCTTAAAATTTTCTGAGATTTCTTTGGAGAAGAGAGAACGAATTCTCAAGAATTGGTCTATGGCTCACCACAAGTATACTGTACTTTTGCGAATGACTTTCATGATTATTAAAATTTTCTGCTGCTTCAAATTCTTCTCCAGGGTAACTTTTTCTTTCAATTCTACTCTGAGGTTTCTTTTTACTATAATTTTGTTTTGGTGTTCATATTCAACATTGCTTTAGAATATTCTGGTATGATTGCTAGTTTAACATTTCTTCTGCTCTGTTATTGGTAGGAGTAACAATTTGCTTTAAGAACAGATTCCTGTATGTTAACGCTTTTCTTTTTATTTTTTAAATTTTCTCTTTTTGTCCTGTATGTATAAATACTACTTCCATCCATAAGTTTCTTATTTCTGATGTAGAATGTTTTCATAATATTGAAAACTAAGCTTGTTTCCTTTTCGGAATTTGGCTAAATGTTTCCTCAAGAAATGTGAAAACGGTAGTAAAGAAATTAGAAGAAACAAGCACAATTTTCGAACAGTAAAACAAAAAACGTAAAAGTACAAAAGTGTTATCCAAGGGGGCCTTACTCATTTCTTTTCCAGTTGGCCTCTTCACTATTTGTTTTCACCTATATTGCCATTTATAGGGTTCCTCTTTTTGAATATTTAGTTAGGAGTTATAGGGTTCCTCTTTTTGGTATTGACTATTGAGTACTATTATACAGAGGATTCTTTTCTGTTATGTTTCAGATCGATGAGGGAACGAAAAACGTTGCATGGAAAGGGATGGGGTATAAAGTAGACACCCGAAAACAGTTGAACCATACCCAAAAAGAGCGGCCTCTTGAAAGGGGAATGATAGAAACTGGCAATGAAGATGATTCCACTCTTCTCCGCTCTCTTTCACAGAAAGGCCTCGAAGTAATTGAAGATAAAAAACATGGTAATGCCTATAAGATCAAATGTGATGTTGTGATTGTTGGCTCGGGTTGCGGTGGTGGAGTGGCTGCTGCGGTTCTTGCAAAATCGGGTTTGAAGGTAGTCGTTCTCGAGAAAGGGAACTATTTCGTGGCTGAAGATTATTCTTCCCTAGAAGGTCCATCCATGAATGAACTATATGAGTCAGGTGGACTCAAGTCAACTCTCGATGGGAAGGTTATGCTGTTGGCTGGAACTACTGTGGGTGGAGGCTCTGCTATAAACTGGTCTGCATCAATTAAAACTCCAAAATCCATTCTCGATGAATGGTCTACTGATTATAAAATTCCTTTGTACGGGAGCATCGATTACCAATTGGCAATGGATGCAGTGTGTGATAGAATTGGAGTGACGGAGGAATGTCCTGTAGAAAGCTTTCAAAATCAAGTTCTTCGAAAAGGATGTGAGAATCTTGGTTTAAAAGTCGACTCGGTTTCGAGAAATTCCTCAAAAGATCATTATTGTGGCTCTTGCTGTTATGGGTGTAGAGTTGGTGACAAGAAAGGGACTGATACTACTTGGCTGGTTGATGCTGTTGAATATGGTGCAGTAATCCTCACAGGCTGCCAAGCTGACAAATTCATTTTGGACAACACAAATAACACAAGTAAAAGAAAAAGATGCATAGGAGTGATTGCAACTCCGCGAAGCAAAAACATCACAAAAAAACTCCATATCGAGGCGAAAGCAACAATCTCTGCTTGTGGTTCTCTCCTGACACCCCCTCTACTTATTGCGAGCGGATTGCGTAATAAAAACATAGGCACCAATCTTCATTTGCATCCAGTTTCTTTGGTTTGGGGGTACTTCCCCGAAGAACTATCCGATTTTAAGGGTAAAATATATGAGGGAGGGCTCATTACTTCCCTGCACAAGGTAGTCTCAAAATATTCCAATAGCCATGCCATAATTGAAACCCCTGCAGTTGGACCGGCCTCATTTGCTGCACTGTTTCCATGGACTTCCGGACGCGAAATGAAAGATGCAATGGTGAAGTACTCTCGAACTGCTCACCTTTTCGCTTTGATAAGAGACCAAGGTGCAGGAGAAATAAAGGTGAAGGCCAATGTCAAGTACCGACTCAACCAAACTGATAAAGAAAATCTAAGAATCGGTTTGAGGCAAGCATTGAGAATCTTGATTGCAGCAGGAGCAACTGAGGTTGGCACGTATAGAAGTGATGGCCAAAGAATCAGATGTCAAGGTACAAACAAGGAAGAGTTGGAGGAGTTCCTTGACTCAGTTGAGGCAGTCGGGGGTCCGTTGTCGAGGCAAAAGTACTGGACCATGTACTGCTCAGCACATCAGATGAGCAGCTGCAGAATGGGAAGCACAAAGGAAGAAGGAGCAGTGGATGAAAATGGTGAAAGTTGGGAAGCAGAAGGTTTATTTGTATGTGATGGAAGTGTGTTACCAACAGCAGTTGGAGTGAATCCAATGATCACAATTCAATCCACAGCCTATTGCATCTCAAAGAAAATTGCAGAATCTTTGAACTAGAGAACAAACTTCATGATAGATGTTTCAATGTTCTTCATTTAGTATTTATTATCTAAAATAAAATAAAAATAAAAATAAAGAAAAGGAAAAGTTCTTTATTTAGTTCAATCTGTTCTTCTAGGAAAACTTTTTTCACTTACTAGTGAAAATAAATGTGATATCAAATATTTAAATTTCAGTTCAAGAATATGTTTTAGAGGTCAGTAAATATGTTTATGTGAGATCGATATGTTGTCAACTGCTATAACTAATTGTTAGTTAGAACAAATAACCAATTGTTAGTTATACCTAATTGTTGATATTTTATTTGTTCAATATATTTGTT

Coding sequence (CDS)

ATGTTTGTTTTGATTGGAGATGGCCTTTCTTTCTTAAAATTTTCTGAGATTTCTTTGGAGAAGAGAGAACGAATTCTCAAGAATTGGTCTATGGCTCACCACAAGTATACTGTACTTTTGCGAATGACTTTCATGATTATTAAAATTTTCTGCTGCTTCAAATTCTTCTCCAGGATCGATGAGGGAACGAAAAACGTTGCATGGAAAGGGATGGGGTATAAAGTAGACACCCGAAAACAGTTGAACCATACCCAAAAAGAGCGGCCTCTTGAAAGGGGAATGATAGAAACTGGCAATGAAGATGATTCCACTCTTCTCCGCTCTCTTTCACAGAAAGGCCTCGAAGTAATTGAAGATAAAAAACATGGTAATGCCTATAAGATCAAATGTGATGTTGTGATTGTTGGCTCGGGTTGCGGTGGTGGAGTGGCTGCTGCGGTTCTTGCAAAATCGGGTTTGAAGGTAGTCGTTCTCGAGAAAGGGAACTATTTCGTGGCTGAAGATTATTCTTCCCTAGAAGGTCCATCCATGAATGAACTATATGAGTCAGGTGGACTCAAGTCAACTCTCGATGGGAAGGTTATGCTGTTGGCTGGAACTACTGTGGGTGGAGGCTCTGCTATAAACTGGTCTGCATCAATTAAAACTCCAAAATCCATTCTCGATGAATGGTCTACTGATTATAAAATTCCTTTGTACGGGAGCATCGATTACCAATTGGCAATGGATGCAGTGTGTGATAGAATTGGAGTGACGGAGGAATGTCCTGTAGAAAGCTTTCAAAATCAAGTTCTTCGAAAAGGATGTGAGAATCTTGGTTTAAAAGTCGACTCGGTTTCGAGAAATTCCTCAAAAGATCATTATTGTGGCTCTTGCTGTTATGGGTGTAGAGTTGGTGACAAGAAAGGGACTGATACTACTTGGCTGGTTGATGCTGTTGAATATGGTGCAGTAATCCTCACAGGCTGCCAAGCTGACAAATTCATTTTGGACAACACAAATAACACAAGTAAAAGAAAAAGATGCATAGGAGTGATTGCAACTCCGCGAAGCAAAAACATCACAAAAAAACTCCATATCGAGGCGAAAGCAACAATCTCTGCTTGTGGTTCTCTCCTGACACCCCCTCTACTTATTGCGAGCGGATTGCGTAATAAAAACATAGGCACCAATCTTCATTTGCATCCAGTTTCTTTGGTTTGGGGGTACTTCCCCGAAGAACTATCCGATTTTAAGGGTAAAATATATGAGGGAGGGCTCATTACTTCCCTGCACAAGGTAGTCTCAAAATATTCCAATAGCCATGCCATAATTGAAACCCCTGCAGTTGGACCGGCCTCATTTGCTGCACTGTTTCCATGGACTTCCGGACGCGAAATGAAAGATGCAATGGTGAAGTACTCTCGAACTGCTCACCTTTTCGCTTTGATAAGAGACCAAGGTGCAGGAGAAATAAAGGTGAAGGCCAATGTCAAGTACCGACTCAACCAAACTGATAAAGAAAATCTAAGAATCGGTTTGAGGCAAGCATTGAGAATCTTGATTGCAGCAGGAGCAACTGAGGTTGGCACGTATAGAAGTGATGGCCAAAGAATCAGATGTCAAGGTACAAACAAGGAAGAGTTGGAGGAGTTCCTTGACTCAGTTGAGGCAGTCGGGGGTCCGTTGTCGAGGCAAAAGTACTGGACCATGTACTGCTCAGCACATCAGATGAGCAGCTGCAGAATGGGAAGCACAAAGGAAGAAGGAGCAGTGGATGAAAATGGTGAAAGTTGGGAAGCAGAAGGTTTATTTGTATGTGATGGAAGTGTGTTACCAACAGCAGTTGGAGTGAATCCAATGATCACAATTCAATCCACAGCCTATTGCATCTCAAAGAAAATTGCAGAATCTTTGAACTAG

Protein sequence

MFVLIGDGLSFLKFSEISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGMGYKVDTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCDVVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVMLLAGTTVGGGSAINWSASIKTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGVTEECPVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVDAVEYGAVILTGCQADKFILDNTNNTSKRKRCIGVIATPRSKNITKKLHIEAKATISACGSLLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSKYSNSHAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKANVKYRLNQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVEAVGGPLSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMITIQSTAYCISKKIAESLN
Homology
BLAST of Cucsat.G19362.T1 vs. ExPASy Swiss-Prot
Match: B5WWZ9 (Long-chain-alcohol oxidase FAO2 OS=Lotus japonicus OX=34305 GN=FAO2 PE=2 SV=1)

HSP 1 Score: 832.0 bits (2148), Expect = 4.5e-240
Identity = 414/626 (66.13%), Postives = 503/626 (80.35%), Query Frame = 0

Query: 13  KFSEISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGMG 72
           KFSEI LEKRE ILK WS    K  + LR+ F++ K+ C +  FSR D    N  WK +G
Sbjct: 127 KFSEIPLEKREEILKRWS--REKCWIPLRLVFVLTKLVCFYNLFSRADVNGHNPIWKAIG 186

Query: 73  YKVDTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCDV 132
           Y+VDTR++L  TQK+RPL+ G+IET  E DSTL++SL++KGLEV ED +  N YKIKCD 
Sbjct: 187 YQVDTREKL--TQKKRPLQEGLIETMYETDSTLIQSLTEKGLEVTEDLEQ-NMYKIKCDA 246

Query: 133 VIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDG 192
           VIVGSGCGGGVAAAVLA SG KV++LEKG YFV+ DYSSLEGPSMNELYESGG+  +LDG
Sbjct: 247 VIVGSGCGGGVAAAVLANSGHKVIILEKGEYFVSHDYSSLEGPSMNELYESGGILPSLDG 306

Query: 193 KVMLLAGTTVGGGSAINWSASIKTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGVT 252
           K+M+LAG+T+GG SAINWSA I+TP S+L EWS  +KIPL+ S DYQ AMD VC RIGVT
Sbjct: 307 KMMILAGSTLGGXSAINWSACIRTPDSVLREWSEKHKIPLFASPDYQSAMDTVCRRIGVT 366

Query: 253 EECPVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVDA 312
           E C  ESFQNQ+LR+GC  +G KV+ V+ NSS DHYCGSCCYGCR GDKKGT++TWLVDA
Sbjct: 367 ENCNKESFQNQILRQGCAKIGFKVEPVAINSSADHYCGSCCYGCRTGDKKGTESTWLVDA 426

Query: 313 VEYGAVILTGCQADK--FILDNTNNTSKRKRCIGVI--ATPRSKNITKKLHIEAKATISA 372
           V  GAVILTGC+A+K  F L + +N +KRK C GVI  AT RSK +TKKL IE+K TISA
Sbjct: 427 VGNGAVILTGCKAEKLNFTLKDGDNGTKRKTCSGVIASATWRSK-VTKKLQIESKVTISA 486

Query: 373 CGSLLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVV 432
           CGSL TPPL+I+SGL+N NIG NLHLHP    WGYFPE++++F G  YEGG+ITS+HKV 
Sbjct: 487 CGSLSTPPLMISSGLKNPNIGKNLHLHPCQFAWGYFPEDMTNFSGNNYEGGIITSIHKVF 546

Query: 433 SKYSNS--HAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIK 492
            + S S    IIE PA+GP SF+AL PW SG ++K+ MVKY+RTA+LFAL+RD G+GE+K
Sbjct: 547 EEDSTSTPRIIIEAPALGPGSFSALVPWVSGLDVKERMVKYARTANLFALVRDHGSGEVK 606

Query: 493 VKANVKYRLNQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFL 552
            +  + Y+L++ D+E+L+ GLR+ALRIL+AAGA EVGTYRSDGQRI+C+G  + +LEEFL
Sbjct: 607 AEGRISYKLDKIDRESLQTGLRKALRILVAAGAVEVGTYRSDGQRIKCRGIKESDLEEFL 666

Query: 553 DSVEAVGGPLSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLP 612
           DSV  VGGP SR + WT++ SAHQM+SCRM +T+EEGAVDENGESWEA+GL+VCDGSVLP
Sbjct: 667 DSVRVVGGPSSRNEVWTVFTSAHQMTSCRMSATEEEGAVDENGESWEAKGLYVCDGSVLP 726

Query: 613 TAVGVNPMITIQSTAYCISKKIAESL 633
           +AVGVNPMITIQSTAYCI+  IAESL
Sbjct: 727 SAVGVNPMITIQSTAYCIASNIAESL 746

BLAST of Cucsat.G19362.T1 vs. ExPASy Swiss-Prot
Match: Q94BP3 (Long-chain-alcohol oxidase FAO4B OS=Arabidopsis thaliana OX=3702 GN=FAO4B PE=2 SV=2)

HSP 1 Score: 771.5 bits (1991), Expect = 7.1e-222
Identity = 384/628 (61.15%), Postives = 479/628 (76.27%), Query Frame = 0

Query: 12  LKFSEISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGM 71
           LKFS++ L+KRE IL+NWS     + +  R+TF + K +  F FFS+ DE  KN A + +
Sbjct: 117 LKFSQLPLDKREAILRNWS-RQSGFLLPFRITFFLAKFYTLFYFFSQTDENLKNPALEAI 176

Query: 72  GYKVD-----TRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAY 131
           GY +D     + K+    +K RPLE+G+IET +E D T+ +SL++KG+ V  D    N Y
Sbjct: 177 GYCIDGTERSSNKKSEADEKRRPLEKGIIETMHESDVTITQSLTEKGVHVARD-DGDNVY 236

Query: 132 KIKCDVVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGL 191
           +I+CD V+VGSG GGGVAAA LAK+GLKV+VLEKGNYF A DYS LE PSM ELYE GGL
Sbjct: 237 RIRCDAVVVGSGSGGGVAAANLAKAGLKVLVLEKGNYFTAHDYSGLEVPSMLELYEKGGL 296

Query: 192 KSTLDGKVMLLAGTTVGGGSAINWSASIKTPKSILDEWSTDYKIPLYGSIDYQLAMDAVC 251
            +T+DGK MLLAG+ VGGG+A+NWSASI+TP  +L EWS   KI  +GS +YQ AMD V 
Sbjct: 297 LTTVDGKFMLLAGSAVGGGTAVNWSASIRTPDHVLQEWSEGSKIKFFGSQEYQSAMDEVT 356

Query: 252 DRIGVTEECPVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDT 311
            RIGVTE C    FQNQVLRKGCE LGL+V+SV RNS +DHYCG C YGCR G K GTD 
Sbjct: 357 IRIGVTERCVKHGFQNQVLRKGCERLGLQVESVPRNSPEDHYCGLCGYGCRAGAKNGTDQ 416

Query: 312 TWLVDAVEYGAVILTGCQADKFIL-DNTNNTSKR-KRCIGVIATPRSKNITKKLHIEAKA 371
           TWLVDAVE GAVILTG +A++F+L DNT+++++R KRC+GV A+     I KK  IEA+ 
Sbjct: 417 TWLVDAVENGAVILTGIKAERFVLVDNTSSSNERKKRCVGVFASSVGGKIGKKFIIEARV 476

Query: 372 TISACGSLLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSL 431
           T+S+ GSLLTPPL+++SGL+N NIG NL LHPV + WGYFPE+ S+F GK+YEGG+ITS+
Sbjct: 477 TVSSAGSLLTPPLMLSSGLKNPNIGRNLKLHPVLMTWGYFPEKDSEFSGKMYEGGIITSV 536

Query: 432 HKVVSKYSNSHAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGE 491
           H +    S   AI+E P +GPAS+A L PW SG ++K+ M+KY RTAHLFAL+RD G+GE
Sbjct: 537 HHMNDTESGCKAILENPLIGPASYAGLSPWVSGPDLKERMIKYGRTAHLFALVRDLGSGE 596

Query: 492 IKVKANVKYRLNQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEE 551
           + ++  V YR  + D+ENLR GLRQALR+ +AAGA EVGTYRSDGQ+++C+   KE +EE
Sbjct: 597 VMMENEVTYRTTKKDRENLRAGLRQALRVSVAAGAVEVGTYRSDGQKMKCEAITKEAMEE 656

Query: 552 FLDSVEAVGGPLSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSV 611
           FLD V+AVGG  ++ +YWT Y SAHQM SCRMG T EEGA+DENGESWEAEGLFVCDGS+
Sbjct: 657 FLDEVDAVGGVGTKGEYWTTYFSAHQMGSCRMGVTAEEGALDENGESWEAEGLFVCDGSI 716

Query: 612 LPTAVGVNPMITIQSTAYCISKKIAESL 633
           LP+AVGVNPMITIQSTAYCIS KI +SL
Sbjct: 717 LPSAVGVNPMITIQSTAYCISSKIVDSL 742

BLAST of Cucsat.G19362.T1 vs. ExPASy Swiss-Prot
Match: B5WWZ8 (Long-chain-alcohol oxidase FAO1 OS=Lotus japonicus OX=34305 GN=FAO1 PE=1 SV=1)

HSP 1 Score: 760.0 bits (1961), Expect = 2.2e-218
Identity = 364/624 (58.33%), Postives = 484/624 (77.56%), Query Frame = 0

Query: 14  FSEISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGMGY 73
           FS +SL+KRER+++ W    +++   +R+ F  +K+ C F +F+ +DE   N AWK +GY
Sbjct: 124 FSSMSLKKRERVMQWW--LKNRFITPIRLAFAYLKVLCLFAYFTWVDENGDNPAWKAIGY 183

Query: 74  KVDTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCDVV 133
           +V   ++L ++   R LE+G++ET +E +STL +SL++KGL V  D ++ N  K+KCD +
Sbjct: 184 EVSADEKLPNSSNGRLLEKGIVETMHETNSTLQQSLTEKGLNVTLDSRN-NILKVKCDAI 243

Query: 134 IVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGK 193
           +VGSGCGGGVAA+VL+ +G KVVVLEKGNYF   DYSSLEGPSM++LYE+GG+ +++D +
Sbjct: 244 VVGSGCGGGVAASVLSGAGHKVVVLEKGNYFAPRDYSSLEGPSMDQLYETGGILASVDSR 303

Query: 194 VMLLAGTTVGGGSAINWSASIKTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGVTE 253
           ++LLAG+TVGGGSA+NWSA IKTP  +L EWS ++ + L+ + +Y  AM+ VC+RIGVTE
Sbjct: 304 ILLLAGSTVGGGSAVNWSACIKTPHEVLKEWSENHNLSLFSTSEYLSAMETVCERIGVTE 363

Query: 254 ECPVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVDAV 313
            C  E FQNQVLRKGC+NLGLKVD V RNSS +H+CGSC YGC  G+K+GT  TWLVDAV
Sbjct: 364 SCTHEGFQNQVLRKGCQNLGLKVDYVPRNSSGNHFCGSCGYGCPKGEKQGTQDTWLVDAV 423

Query: 314 EYGAVILTGCQADKFILDNTNNTS-KRKRCIGVIATPRSKNITKKLHIEAKATISACGSL 373
           E GAVI+TGC+A++F+L+N  N S ++K+C+GV+A   S  +T KL IEAK TISA G+L
Sbjct: 424 ENGAVIITGCKAERFLLENNRNGSGRKKKCLGVLAKALSSRVTMKLQIEAKVTISAGGAL 483

Query: 374 LTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSK-- 433
           LTPPL+I+SGL+NKNIG NLHLHPV + WGYFPE  S+ KGKI+EGG+ITS+HKV S+  
Sbjct: 484 LTPPLMISSGLKNKNIGKNLHLHPVLMTWGYFPESTSELKGKIFEGGIITSVHKVPSRDS 543

Query: 434 --YSNSHAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVK 493
             YS++ AIIETP++GP SFA+L PW SG + K+ M+ Y RTAHL  +IRD+  G++  +
Sbjct: 544 NSYSDTRAIIETPSLGPGSFASLCPWESGLDFKERMLNYPRTAHLITIIRDKACGQVTTE 603

Query: 494 ANVKYRLNQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDS 553
             V Y+L+  DKEN++ GL+QALRIL AAGA EVGT+RSDGQR++C   ++ E+ EF+DS
Sbjct: 604 GRVSYKLSAFDKENMKCGLQQALRILKAAGAVEVGTHRSDGQRVKCGEVSENEMAEFIDS 663

Query: 554 VEAVGGPLSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTA 613
           V  + G LS  + W +Y SAHQM SCRMG  ++EGAVDENGESWEA+GLFVCD S+LPTA
Sbjct: 664 VCPMEGALSPGENWNIYTSAHQMGSCRMGMNEKEGAVDENGESWEAQGLFVCDASLLPTA 723

Query: 614 VGVNPMITIQSTAYCISKKIAESL 633
           VGVNPMITIQSTAYCIS ++ + L
Sbjct: 724 VGVNPMITIQSTAYCISNRVVDYL 744

BLAST of Cucsat.G19362.T1 vs. ExPASy Swiss-Prot
Match: Q9LW56 (Long-chain-alcohol oxidase FAO3 OS=Arabidopsis thaliana OX=3702 GN=FAO3 PE=1 SV=1)

HSP 1 Score: 750.7 bits (1937), Expect = 1.3e-215
Identity = 369/630 (58.57%), Postives = 479/630 (76.03%), Query Frame = 0

Query: 13  KFSEISLEKRERIL----KNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAW 72
           KFSE+SLEKRER+L    KNW +        +R  F+ IK+   F FFSR++   +N AW
Sbjct: 118 KFSELSLEKRERVLQKQFKNWILTP------IRAAFVYIKVAFLFCFFSRVNPNGENPAW 177

Query: 73  KGMGYKVD-TRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYK 132
           + +GY+V+    + + T  ERPLE+G++ET  E + TLL SL+ KGLE + D +H +A +
Sbjct: 178 EAIGYRVNPDENKPSETHNERPLEKGIVETMEETEQTLLESLAHKGLEAVLDTEH-DAIR 237

Query: 133 IKCDVVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLK 192
           IKCDVV+VGSG GGGVAA+VLAKSGLKVVVLEKG+YF   ++   EGP +++LYE+GG+ 
Sbjct: 238 IKCDVVVVGSGSGGGVAASVLAKSGLKVVVLEKGSYFTPSEHRPFEGPGLDKLYENGGIL 297

Query: 193 STLDGKVMLLAGTTVGGGSAINWSASIKTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCD 252
            ++DG  M+LAG TVGGGSA+NWSA IKTPKS+L EWS D  IPL+G+ +Y  AM+ V  
Sbjct: 298 PSVDGSFMVLAGATVGGGSAVNWSACIKTPKSVLQEWSEDQNIPLFGTKEYLTAMEVVWK 357

Query: 253 RIGVTEECPVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTT 312
           R+GVTE+C +ESFQNQ+LRKGCENLG  V++V RNSS+ HYCGSC YGCR GDKKG+D T
Sbjct: 358 RMGVTEKCELESFQNQILRKGCENLGFNVENVPRNSSESHYCGSCGYGCRQGDKKGSDRT 417

Query: 313 WLVDAVEYGAVILTGCQADKFILD--NTNNTSKRKRCIGVIATPRSKNITKKLHIEAKAT 372
           WLVDAV +GAVILTGC+A++FIL+   +N   K+ +C+GV+A   + NI K L IEAK T
Sbjct: 418 WLVDAVGHGAVILTGCKAERFILEKNGSNKGGKQMKCLGVMAKSLNGNIAKMLKIEAKVT 477

Query: 373 ISACGSLLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELS---DFKGKIYEGGLIT 432
           +SA G+LLTPPL+I+SGLRN+NIG NLHLHPV + WGYFP++ S    FKG  YEGG+IT
Sbjct: 478 VSAGGALLTPPLMISSGLRNRNIGKNLHLHPVLMAWGYFPDKESSNISFKGNSYEGGIIT 537

Query: 433 SLHKVVSKYSNSHAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGA 492
           S+ KV+S+ S   AIIETP +GP SF+ L PWTSG +MK  M +YSRTA L  ++RD+G+
Sbjct: 538 SVSKVLSEDSEVRAIIETPQLGPGSFSVLTPWTSGLDMKKRMARYSRTASLITIVRDRGS 597

Query: 493 GEIKVKANVKYRLNQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEEL 552
           GE+K +  + Y +++TD++NL+ GLR++LRILIAAGA EVGT+RSDGQR+ C+G N+  +
Sbjct: 598 GEVKTEGRINYTVDKTDRDNLKAGLRESLRILIAAGAEEVGTHRSDGQRLICKGVNENSI 657

Query: 553 EEFLDSVEAVGGPLSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDG 612
           +EFLDSV    G     + W +Y SAHQM SCR+G  ++EGA+D NGESWEAE LFVCD 
Sbjct: 658 QEFLDSVSTEEGAKGMTEKWNVYSSAHQMGSCRIGENEKEGAIDLNGESWEAEKLFVCDA 717

Query: 613 SVLPTAVGVNPMITIQSTAYCISKKIAESL 633
           S LP+AVGVNPMIT+ STAYCIS +IA+S+
Sbjct: 718 SALPSAVGVNPMITVMSTAYCISTRIAKSM 740

BLAST of Cucsat.G19362.T1 vs. ExPASy Swiss-Prot
Match: Q9ZWB9 (Long-chain-alcohol oxidase FAO1 OS=Arabidopsis thaliana OX=3702 GN=FAO1 PE=3 SV=2)

HSP 1 Score: 714.9 bits (1844), Expect = 7.9e-205
Identity = 358/625 (57.28%), Postives = 461/625 (73.76%), Query Frame = 0

Query: 12  LKFSEISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGM 71
           LKFSE+SLEKRE++L+ W+     Y  L R+ FM+IK    F +F+  +E ++N  W  +
Sbjct: 131 LKFSEMSLEKREKVLQRWNT--QWYNPLARIGFMMIKAIFLFYYFTWTNENSENPVWDAI 190

Query: 72  GYKVDTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCD 131
            Y V+  +  +  QKERPL+ G+IET  ED+ T+ + +  KGL+V ED++  + YKI+CD
Sbjct: 191 NYSVEIGENEDMEQKERPLDEGIIETAKEDEMTIKQRMINKGLKVTEDRER-DTYKIECD 250

Query: 132 VVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLD 191
            V+VGSGCGGGVAAA+LAKSGL+VVV+EKGNYF   DYS+LEGPSM EL+ES  L  T D
Sbjct: 251 AVVVGSGCGGGVAAAILAKSGLRVVVIEKGNYFAPRDYSALEGPSMFELFESNSLMMTHD 310

Query: 192 GKVMLLAGTTVGGGSAINWSASIKTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGV 251
           G+   +AG+TVGGGS +NW+AS+KTP +I++EWS    I +Y S  Y+ AM  VC R+GV
Sbjct: 311 GRFRFMAGSTVGGGSVVNWAASLKTPDAIIEEWSVHRGISIYSSEKYKAAMGIVCKRLGV 370

Query: 252 TEECPVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVD 311
           TE+   E FQNQ+LRKGCE LGL V  V RNS++ HYCGSC YGC  G+K+GTD+TWLVD
Sbjct: 371 TEKIIREGFQNQILRKGCEKLGLDVTIVPRNSTEKHYCGSCSYGCPTGEKRGTDSTWLVD 430

Query: 312 AVEYGAVILTGCQADKFIL-DNTNN----TSKRKRCIGVIATPRSKNITKKLHIEAKATI 371
           AV   AVILT C+A+K IL DN  N    + +RKRC+GV A+  S    KKL I AK TI
Sbjct: 431 AVNNNAVILTQCKAEKLILADNDANKREESGRRKRCLGVAAS-LSHQTRKKLQINAKVTI 490

Query: 372 SACGSLLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHK 431
            ACGSL TP LL +SGL+N NI   LH+HP+ + WGYFPE+ S+ +G  +EG ++TSLH 
Sbjct: 491 VACGSLKTPGLLASSGLKNSNISRGLHIHPIMMAWGYFPEKNSELEGAAHEGEIVTSLHY 550

Query: 432 VVSKYSNSHAI-IETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEI 491
           V    S +  I +ETPA+GP +FAAL PW SG +MK+ M KY+RTAH+FA++RD+G GE+
Sbjct: 551 VHPMDSTTPNITLETPAIGPGTFAALTPWVSGSDMKERMAKYARTAHIFAMVRDEGVGEV 610

Query: 492 KVKANVKYRLNQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEF 551
           K    VKYRL + D+ENL IGL+QALRIL+AAGA EVGTYRSDGQR++C G  +++LE F
Sbjct: 611 KGDI-VKYRLTKADEENLTIGLKQALRILVAAGAAEVGTYRSDGQRMKCDGIKQKDLEAF 670

Query: 552 LDSVEAVGGPLSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVL 611
           LD+V A  G +S  K+WT   +AHQ+  CRMG+T++EGA+D  GESWEAE L+VCD SVL
Sbjct: 671 LDTVNAPPGVVSMSKHWTQSFTAHQIGCCRMGATEKEGAIDGKGESWEAEDLYVCDASVL 730

Query: 612 PTAVGVNPMITIQSTAYCISKKIAE 631
           PTA+GVNPMIT+QSTAYCIS +IAE
Sbjct: 731 PTALGVNPMITVQSTAYCISNRIAE 750

BLAST of Cucsat.G19362.T1 vs. NCBI nr
Match: XP_004152057.1 (long-chain-alcohol oxidase FAO2 [Cucumis sativus] >KGN58167.1 hypothetical protein Csa_017539 [Cucumis sativus])

HSP 1 Score: 1252 bits (3239), Expect = 0.0
Identity = 623/623 (100.00%), Postives = 623/623 (100.00%), Query Frame = 0

Query: 11  FLKFSEISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKG 70
           FLKFSEISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKG
Sbjct: 116 FLKFSEISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKG 175

Query: 71  MGYKVDTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKC 130
           MGYKVDTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKC
Sbjct: 176 MGYKVDTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKC 235

Query: 131 DVVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTL 190
           DVVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTL
Sbjct: 236 DVVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTL 295

Query: 191 DGKVMLLAGTTVGGGSAINWSASIKTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIG 250
           DGKVMLLAGTTVGGGSAINWSASIKTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIG
Sbjct: 296 DGKVMLLAGTTVGGGSAINWSASIKTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIG 355

Query: 251 VTEECPVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLV 310
           VTEECPVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLV
Sbjct: 356 VTEECPVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLV 415

Query: 311 DAVEYGAVILTGCQADKFILDNTNNTSKRKRCIGVIATPRSKNITKKLHIEAKATISACG 370
           DAVEYGAVILTGCQADKFILDNTNNTSKRKRCIGVIATPRSKNITKKLHIEAKATISACG
Sbjct: 416 DAVEYGAVILTGCQADKFILDNTNNTSKRKRCIGVIATPRSKNITKKLHIEAKATISACG 475

Query: 371 SLLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSK 430
           SLLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSK
Sbjct: 476 SLLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSK 535

Query: 431 YSNSHAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKAN 490
           YSNSHAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKAN
Sbjct: 536 YSNSHAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKAN 595

Query: 491 VKYRLNQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVE 550
           VKYRLNQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVE
Sbjct: 596 VKYRLNQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVE 655

Query: 551 AVGGPLSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAVG 610
           AVGGPLSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAVG
Sbjct: 656 AVGGPLSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAVG 715

Query: 611 VNPMITIQSTAYCISKKIAESLN 633
           VNPMITIQSTAYCISKKIAESLN
Sbjct: 716 VNPMITIQSTAYCISKKIAESLN 738

BLAST of Cucsat.G19362.T1 vs. NCBI nr
Match: KAA0037980.1 (long-chain-alcohol oxidase FAO2-like [Cucumis melo var. makuwa])

HSP 1 Score: 1206 bits (3121), Expect = 0.0
Identity = 596/623 (95.67%), Postives = 611/623 (98.07%), Query Frame = 0

Query: 11  FLKFSEISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKG 70
           FLKFSEISLEKRE+ILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWK 
Sbjct: 116 FLKFSEISLEKREQILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKA 175

Query: 71  MGYKVDTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKC 130
           MGY+VDTRKQLNHTQKERPLERGMIETGNEDDSTL RSLSQKGLEVIEDKKHGNAYKIKC
Sbjct: 176 MGYEVDTRKQLNHTQKERPLERGMIETGNEDDSTLFRSLSQKGLEVIEDKKHGNAYKIKC 235

Query: 131 DVVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTL 190
           DVVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTL
Sbjct: 236 DVVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTL 295

Query: 191 DGKVMLLAGTTVGGGSAINWSASIKTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIG 250
           DGKVMLLAGTTVGGGSAINWSASI+TPKS+LDEWSTD+KIPLYGSIDYQLAMDAVCDRIG
Sbjct: 296 DGKVMLLAGTTVGGGSAINWSASIRTPKSVLDEWSTDHKIPLYGSIDYQLAMDAVCDRIG 355

Query: 251 VTEECPVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLV 310
           VT  C VESFQNQVLRKGCENLGLKVDSVSRNSS+DHYCGSCCYGCRVGDKKGTDTTWLV
Sbjct: 356 VTRGCRVESFQNQVLRKGCENLGLKVDSVSRNSSEDHYCGSCCYGCRVGDKKGTDTTWLV 415

Query: 311 DAVEYGAVILTGCQADKFILDNTNNTSKRKRCIGVIATPRSKNITKKLHIEAKATISACG 370
           DAV+ GAVILTGC ADKFIL+NTNNTSK+K+CIGVIATP SKN+TKKLHIEAKATISACG
Sbjct: 416 DAVKCGAVILTGCHADKFILENTNNTSKKKKCIGVIATPLSKNVTKKLHIEAKATISACG 475

Query: 371 SLLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSK 430
           SLLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLIT LH VVSK
Sbjct: 476 SLLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITCLHNVVSK 535

Query: 431 YSNSHAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKAN 490
           YSNSHAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFAL+RDQGAGEIKVKAN
Sbjct: 536 YSNSHAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALVRDQGAGEIKVKAN 595

Query: 491 VKYRLNQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVE 550
           VKYRLNQTDKENL+IGLRQALRILIAAGATE+GTYRSDGQRIRCQGT KE+LEEFLDSVE
Sbjct: 596 VKYRLNQTDKENLKIGLRQALRILIAAGATEIGTYRSDGQRIRCQGTKKEDLEEFLDSVE 655

Query: 551 AVGGPLSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAVG 610
           AVGGP SRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAVG
Sbjct: 656 AVGGPWSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAVG 715

Query: 611 VNPMITIQSTAYCISKKIAESLN 633
           VNPMITIQSTAYCISKKIAESLN
Sbjct: 716 VNPMITIQSTAYCISKKIAESLN 738

BLAST of Cucsat.G19362.T1 vs. NCBI nr
Match: XP_008447391.2 (PREDICTED: LOW QUALITY PROTEIN: long-chain-alcohol oxidase FAO2-like [Cucumis melo])

HSP 1 Score: 1204 bits (3115), Expect = 0.0
Identity = 595/623 (95.51%), Postives = 610/623 (97.91%), Query Frame = 0

Query: 11  FLKFSEISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKG 70
           FLKFSEISLEKRE+ILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWK 
Sbjct: 116 FLKFSEISLEKREQILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKA 175

Query: 71  MGYKVDTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKC 130
           MGY+VDTRKQLNHTQKERPLERGMIETGNEDDSTL RSLSQKGLEVIEDKKHGNAYKIKC
Sbjct: 176 MGYEVDTRKQLNHTQKERPLERGMIETGNEDDSTLFRSLSQKGLEVIEDKKHGNAYKIKC 235

Query: 131 DVVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTL 190
           DVVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTL
Sbjct: 236 DVVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTL 295

Query: 191 DGKVMLLAGTTVGGGSAINWSASIKTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIG 250
           DGKVMLLAGTTVGGGSAINWSASI+TPKS+LDEWSTD+KIPLYGSIDYQLAMDAVCDRIG
Sbjct: 296 DGKVMLLAGTTVGGGSAINWSASIRTPKSVLDEWSTDHKIPLYGSIDYQLAMDAVCDRIG 355

Query: 251 VTEECPVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLV 310
           VT  C VESFQNQVLRKGCENLGLKVDSVSRNSS+DHYCGSCCYGCRVGDKKGTDTTWLV
Sbjct: 356 VTRGCRVESFQNQVLRKGCENLGLKVDSVSRNSSEDHYCGSCCYGCRVGDKKGTDTTWLV 415

Query: 311 DAVEYGAVILTGCQADKFILDNTNNTSKRKRCIGVIATPRSKNITKKLHIEAKATISACG 370
           DAV+ GAVILTGC ADKFIL+NTNNTSK+ +CIGVIATP SKN+TKKLHIEAKATISACG
Sbjct: 416 DAVKCGAVILTGCHADKFILENTNNTSKKXKCIGVIATPLSKNVTKKLHIEAKATISACG 475

Query: 371 SLLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSK 430
           SLLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLIT LH VVSK
Sbjct: 476 SLLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITCLHNVVSK 535

Query: 431 YSNSHAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKAN 490
           YSNSHAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFAL+RDQGAGEIKVKAN
Sbjct: 536 YSNSHAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALVRDQGAGEIKVKAN 595

Query: 491 VKYRLNQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVE 550
           VKYRLNQTDKENL+IGLRQALRILIAAGATE+GTYRSDGQRIRCQGT KE+LEEFLDSVE
Sbjct: 596 VKYRLNQTDKENLKIGLRQALRILIAAGATEIGTYRSDGQRIRCQGTKKEDLEEFLDSVE 655

Query: 551 AVGGPLSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAVG 610
           AVGGP SRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAVG
Sbjct: 656 AVGGPWSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAVG 715

Query: 611 VNPMITIQSTAYCISKKIAESLN 633
           VNPMITIQSTAYCISKKIAESLN
Sbjct: 716 VNPMITIQSTAYCISKKIAESLN 738

BLAST of Cucsat.G19362.T1 vs. NCBI nr
Match: XP_038888257.1 (long-chain-alcohol oxidase FAO2-like [Benincasa hispida])

HSP 1 Score: 1154 bits (2985), Expect = 0.0
Identity = 572/622 (91.96%), Postives = 597/622 (95.98%), Query Frame = 0

Query: 12  LKFSEISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGM 71
           LKFSEIS+EKRE ILKNWSMAH KYTVLLRMTFMIIKIFCCFKFFSRID  TKNVAWK M
Sbjct: 117 LKFSEISVEKREEILKNWSMAHQKYTVLLRMTFMIIKIFCCFKFFSRIDGETKNVAWKAM 176

Query: 72  GYKVDTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCD 131
           GY VDTRK+LN T+KERPLERGMIETGNEDDST  +SL+ KGLEV EDKKHGNAYKIKCD
Sbjct: 177 GYHVDTRKRLNDTRKERPLERGMIETGNEDDSTFPKSLTLKGLEVTEDKKHGNAYKIKCD 236

Query: 132 VVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLD 191
           VVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLD
Sbjct: 237 VVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLD 296

Query: 192 GKVMLLAGTTVGGGSAINWSASIKTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGV 251
           GKVMLLAGTTVGGGSAINWSASI+TPKS+LDEWSTD+KIPL+ SIDYQLAMDAVCDRIGV
Sbjct: 297 GKVMLLAGTTVGGGSAINWSASIRTPKSVLDEWSTDHKIPLFRSIDYQLAMDAVCDRIGV 356

Query: 252 TEECPVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVD 311
            E C +ESFQNQVLRKGCENLGLKVD VSRNSSKDHYCGSCCYGCRVGDKKGTD+TWLVD
Sbjct: 357 IEGCSLESFQNQVLRKGCENLGLKVDLVSRNSSKDHYCGSCCYGCRVGDKKGTDSTWLVD 416

Query: 312 AVEYGAVILTGCQADKFILDNTNNTSKRKRCIGVIATPRSKNITKKLHIEAKATISACGS 371
           AVE GAVILTGCQADKFIL+NTNNTS+ KRC+GVIATP SKNITKKLHIEAKATISACGS
Sbjct: 417 AVESGAVILTGCQADKFILENTNNTSRNKRCLGVIATPLSKNITKKLHIEAKATISACGS 476

Query: 372 LLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSKY 431
           LLTPPLLIASGLRNKNIG+NLHLHPV L WGYFPEELSDF GKIYEGGLITSLHKVVSK 
Sbjct: 477 LLTPPLLIASGLRNKNIGSNLHLHPVILAWGYFPEELSDFTGKIYEGGLITSLHKVVSKD 536

Query: 432 SNSHAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKANV 491
           S+SHAIIETPA+G ASFAALFPW SGREMKDAMVKY+RTAHLFAL+RDQGAGEIKVKANV
Sbjct: 537 SHSHAIIETPALGSASFAALFPWISGREMKDAMVKYARTAHLFALVRDQGAGEIKVKANV 596

Query: 492 KYRLNQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVEA 551
           KYRLNQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRI+CQGTNKE+LEEFLDSVEA
Sbjct: 597 KYRLNQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIKCQGTNKEDLEEFLDSVEA 656

Query: 552 VGGPLSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAVGV 611
           VGGPLSR+K+WTMYCSAHQMSSC+MGSTKEEGAVDENGESWEAEGLFVCDGSVLPTA+GV
Sbjct: 657 VGGPLSREKHWTMYCSAHQMSSCKMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAIGV 716

Query: 612 NPMITIQSTAYCISKKIAESLN 633
           NPMITIQSTAYCISKKIAESLN
Sbjct: 717 NPMITIQSTAYCISKKIAESLN 738

BLAST of Cucsat.G19362.T1 vs. NCBI nr
Match: XP_022967423.1 (long-chain-alcohol oxidase FAO2-like [Cucurbita maxima] >XP_022967424.1 long-chain-alcohol oxidase FAO2-like [Cucurbita maxima])

HSP 1 Score: 1105 bits (2857), Expect = 0.0
Identity = 539/622 (86.66%), Postives = 585/622 (94.05%), Query Frame = 0

Query: 12  LKFSEISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGM 71
           LKFSEIS EKRE+ILK+WS+AHH+YTVLLRM FMIIK+FCCFKFFSRID  +KN+AW+ M
Sbjct: 117 LKFSEISPEKREQILKDWSVAHHRYTVLLRMVFMIIKMFCCFKFFSRIDRESKNIAWEAM 176

Query: 72  GYKVDTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCD 131
           GY VDTRK+LN ++KERPLERG+IETG EDDSTLLRSLS KGL V ED+KHG+ Y IKCD
Sbjct: 177 GYHVDTRKRLNDSRKERPLERGLIETGKEDDSTLLRSLSMKGLAVTEDQKHGSTYTIKCD 236

Query: 132 VVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLD 191
           VVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFV EDYSSLEGPSMNELYE+GGLKSTLD
Sbjct: 237 VVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVPEDYSSLEGPSMNELYETGGLKSTLD 296

Query: 192 GKVMLLAGTTVGGGSAINWSASIKTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGV 251
           GKVMLLAGTTVGGGSAINWSASI+TPK +L+EWST +KIPL+GS DYQ AMDAVCDRIGV
Sbjct: 297 GKVMLLAGTTVGGGSAINWSASIRTPKPVLEEWSTIHKIPLFGSFDYQSAMDAVCDRIGV 356

Query: 252 TEECPVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVD 311
           TE C +ESFQNQVLRKGCENLGLKVDSV RNSSKDHYCGSCCYGCRVGDKKGTD+TWLVD
Sbjct: 357 TEGCRLESFQNQVLRKGCENLGLKVDSVPRNSSKDHYCGSCCYGCRVGDKKGTDSTWLVD 416

Query: 312 AVEYGAVILTGCQADKFILDNTNNTSKRKRCIGVIATPRSKNITKKLHIEAKATISACGS 371
           AVE GAVILTGC+ADK +L+NTNNTS+ KRC+GVIATP SKN+TKKLHIEAKATISACGS
Sbjct: 417 AVESGAVILTGCRADKLVLENTNNTSRNKRCLGVIATPLSKNVTKKLHIEAKATISACGS 476

Query: 372 LLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSKY 431
           LLTPPLLIASGLRNKNIG+NLHLHPV L WGYFPE+LSDF GKIYEGGLITSLH VVSK 
Sbjct: 477 LLTPPLLIASGLRNKNIGSNLHLHPVILAWGYFPEQLSDFTGKIYEGGLITSLHNVVSKD 536

Query: 432 SNSHAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKANV 491
           SNSHAII+TPA+GPASFAA+FPWTSGR+MKDA+VKY+RTAHLFAL+RD+GAGEIKVKANV
Sbjct: 537 SNSHAIIQTPALGPASFAAMFPWTSGRDMKDAVVKYARTAHLFALVRDRGAGEIKVKANV 596

Query: 492 KYRLNQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVEA 551
           KYRL+QTDKENLRIGLRQALRILIAAGATEVGTYRSDGQR +  GTNKE+LEEF DSVEA
Sbjct: 597 KYRLDQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRFQFHGTNKEDLEEFFDSVEA 656

Query: 552 VGGPLSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAVGV 611
            GGP SR+KYW +YCSAHQMSSCRMGST+EEGAVDENGESWEA+GLFVCDGSVLPTAVGV
Sbjct: 657 AGGPFSREKYWNIYCSAHQMSSCRMGSTEEEGAVDENGESWEAKGLFVCDGSVLPTAVGV 716

Query: 612 NPMITIQSTAYCISKKIAESLN 633
           NPMITIQSTAYCISKK+AESLN
Sbjct: 717 NPMITIQSTAYCISKKLAESLN 738

BLAST of Cucsat.G19362.T1 vs. ExPASy TrEMBL
Match: A0A0A0LBV7 (Long-chain-alcohol oxidase OS=Cucumis sativus OX=3659 GN=Csa_3G575330 PE=3 SV=1)

HSP 1 Score: 1252 bits (3239), Expect = 0.0
Identity = 623/623 (100.00%), Postives = 623/623 (100.00%), Query Frame = 0

Query: 11  FLKFSEISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKG 70
           FLKFSEISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKG
Sbjct: 116 FLKFSEISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKG 175

Query: 71  MGYKVDTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKC 130
           MGYKVDTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKC
Sbjct: 176 MGYKVDTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKC 235

Query: 131 DVVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTL 190
           DVVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTL
Sbjct: 236 DVVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTL 295

Query: 191 DGKVMLLAGTTVGGGSAINWSASIKTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIG 250
           DGKVMLLAGTTVGGGSAINWSASIKTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIG
Sbjct: 296 DGKVMLLAGTTVGGGSAINWSASIKTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIG 355

Query: 251 VTEECPVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLV 310
           VTEECPVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLV
Sbjct: 356 VTEECPVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLV 415

Query: 311 DAVEYGAVILTGCQADKFILDNTNNTSKRKRCIGVIATPRSKNITKKLHIEAKATISACG 370
           DAVEYGAVILTGCQADKFILDNTNNTSKRKRCIGVIATPRSKNITKKLHIEAKATISACG
Sbjct: 416 DAVEYGAVILTGCQADKFILDNTNNTSKRKRCIGVIATPRSKNITKKLHIEAKATISACG 475

Query: 371 SLLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSK 430
           SLLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSK
Sbjct: 476 SLLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSK 535

Query: 431 YSNSHAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKAN 490
           YSNSHAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKAN
Sbjct: 536 YSNSHAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKAN 595

Query: 491 VKYRLNQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVE 550
           VKYRLNQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVE
Sbjct: 596 VKYRLNQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVE 655

Query: 551 AVGGPLSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAVG 610
           AVGGPLSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAVG
Sbjct: 656 AVGGPLSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAVG 715

Query: 611 VNPMITIQSTAYCISKKIAESLN 633
           VNPMITIQSTAYCISKKIAESLN
Sbjct: 716 VNPMITIQSTAYCISKKIAESLN 738

BLAST of Cucsat.G19362.T1 vs. ExPASy TrEMBL
Match: A0A5A7T3R3 (Long-chain-alcohol oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G001660 PE=3 SV=1)

HSP 1 Score: 1206 bits (3121), Expect = 0.0
Identity = 596/623 (95.67%), Postives = 611/623 (98.07%), Query Frame = 0

Query: 11  FLKFSEISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKG 70
           FLKFSEISLEKRE+ILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWK 
Sbjct: 116 FLKFSEISLEKREQILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKA 175

Query: 71  MGYKVDTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKC 130
           MGY+VDTRKQLNHTQKERPLERGMIETGNEDDSTL RSLSQKGLEVIEDKKHGNAYKIKC
Sbjct: 176 MGYEVDTRKQLNHTQKERPLERGMIETGNEDDSTLFRSLSQKGLEVIEDKKHGNAYKIKC 235

Query: 131 DVVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTL 190
           DVVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTL
Sbjct: 236 DVVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTL 295

Query: 191 DGKVMLLAGTTVGGGSAINWSASIKTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIG 250
           DGKVMLLAGTTVGGGSAINWSASI+TPKS+LDEWSTD+KIPLYGSIDYQLAMDAVCDRIG
Sbjct: 296 DGKVMLLAGTTVGGGSAINWSASIRTPKSVLDEWSTDHKIPLYGSIDYQLAMDAVCDRIG 355

Query: 251 VTEECPVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLV 310
           VT  C VESFQNQVLRKGCENLGLKVDSVSRNSS+DHYCGSCCYGCRVGDKKGTDTTWLV
Sbjct: 356 VTRGCRVESFQNQVLRKGCENLGLKVDSVSRNSSEDHYCGSCCYGCRVGDKKGTDTTWLV 415

Query: 311 DAVEYGAVILTGCQADKFILDNTNNTSKRKRCIGVIATPRSKNITKKLHIEAKATISACG 370
           DAV+ GAVILTGC ADKFIL+NTNNTSK+K+CIGVIATP SKN+TKKLHIEAKATISACG
Sbjct: 416 DAVKCGAVILTGCHADKFILENTNNTSKKKKCIGVIATPLSKNVTKKLHIEAKATISACG 475

Query: 371 SLLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSK 430
           SLLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLIT LH VVSK
Sbjct: 476 SLLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITCLHNVVSK 535

Query: 431 YSNSHAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKAN 490
           YSNSHAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFAL+RDQGAGEIKVKAN
Sbjct: 536 YSNSHAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALVRDQGAGEIKVKAN 595

Query: 491 VKYRLNQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVE 550
           VKYRLNQTDKENL+IGLRQALRILIAAGATE+GTYRSDGQRIRCQGT KE+LEEFLDSVE
Sbjct: 596 VKYRLNQTDKENLKIGLRQALRILIAAGATEIGTYRSDGQRIRCQGTKKEDLEEFLDSVE 655

Query: 551 AVGGPLSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAVG 610
           AVGGP SRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAVG
Sbjct: 656 AVGGPWSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAVG 715

Query: 611 VNPMITIQSTAYCISKKIAESLN 633
           VNPMITIQSTAYCISKKIAESLN
Sbjct: 716 VNPMITIQSTAYCISKKIAESLN 738

BLAST of Cucsat.G19362.T1 vs. ExPASy TrEMBL
Match: A0A1S3BHB1 (Long-chain-alcohol oxidase OS=Cucumis melo OX=3656 GN=LOC103489850 PE=3 SV=1)

HSP 1 Score: 1204 bits (3115), Expect = 0.0
Identity = 595/623 (95.51%), Postives = 610/623 (97.91%), Query Frame = 0

Query: 11  FLKFSEISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKG 70
           FLKFSEISLEKRE+ILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWK 
Sbjct: 116 FLKFSEISLEKREQILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKA 175

Query: 71  MGYKVDTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKC 130
           MGY+VDTRKQLNHTQKERPLERGMIETGNEDDSTL RSLSQKGLEVIEDKKHGNAYKIKC
Sbjct: 176 MGYEVDTRKQLNHTQKERPLERGMIETGNEDDSTLFRSLSQKGLEVIEDKKHGNAYKIKC 235

Query: 131 DVVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTL 190
           DVVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTL
Sbjct: 236 DVVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTL 295

Query: 191 DGKVMLLAGTTVGGGSAINWSASIKTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIG 250
           DGKVMLLAGTTVGGGSAINWSASI+TPKS+LDEWSTD+KIPLYGSIDYQLAMDAVCDRIG
Sbjct: 296 DGKVMLLAGTTVGGGSAINWSASIRTPKSVLDEWSTDHKIPLYGSIDYQLAMDAVCDRIG 355

Query: 251 VTEECPVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLV 310
           VT  C VESFQNQVLRKGCENLGLKVDSVSRNSS+DHYCGSCCYGCRVGDKKGTDTTWLV
Sbjct: 356 VTRGCRVESFQNQVLRKGCENLGLKVDSVSRNSSEDHYCGSCCYGCRVGDKKGTDTTWLV 415

Query: 311 DAVEYGAVILTGCQADKFILDNTNNTSKRKRCIGVIATPRSKNITKKLHIEAKATISACG 370
           DAV+ GAVILTGC ADKFIL+NTNNTSK+ +CIGVIATP SKN+TKKLHIEAKATISACG
Sbjct: 416 DAVKCGAVILTGCHADKFILENTNNTSKKXKCIGVIATPLSKNVTKKLHIEAKATISACG 475

Query: 371 SLLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSK 430
           SLLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLIT LH VVSK
Sbjct: 476 SLLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITCLHNVVSK 535

Query: 431 YSNSHAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKAN 490
           YSNSHAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFAL+RDQGAGEIKVKAN
Sbjct: 536 YSNSHAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALVRDQGAGEIKVKAN 595

Query: 491 VKYRLNQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVE 550
           VKYRLNQTDKENL+IGLRQALRILIAAGATE+GTYRSDGQRIRCQGT KE+LEEFLDSVE
Sbjct: 596 VKYRLNQTDKENLKIGLRQALRILIAAGATEIGTYRSDGQRIRCQGTKKEDLEEFLDSVE 655

Query: 551 AVGGPLSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAVG 610
           AVGGP SRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAVG
Sbjct: 656 AVGGPWSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAVG 715

Query: 611 VNPMITIQSTAYCISKKIAESLN 633
           VNPMITIQSTAYCISKKIAESLN
Sbjct: 716 VNPMITIQSTAYCISKKIAESLN 738

BLAST of Cucsat.G19362.T1 vs. ExPASy TrEMBL
Match: A0A6J1HWN7 (Long-chain-alcohol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111466966 PE=3 SV=1)

HSP 1 Score: 1105 bits (2857), Expect = 0.0
Identity = 539/622 (86.66%), Postives = 585/622 (94.05%), Query Frame = 0

Query: 12  LKFSEISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGM 71
           LKFSEIS EKRE+ILK+WS+AHH+YTVLLRM FMIIK+FCCFKFFSRID  +KN+AW+ M
Sbjct: 117 LKFSEISPEKREQILKDWSVAHHRYTVLLRMVFMIIKMFCCFKFFSRIDRESKNIAWEAM 176

Query: 72  GYKVDTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCD 131
           GY VDTRK+LN ++KERPLERG+IETG EDDSTLLRSLS KGL V ED+KHG+ Y IKCD
Sbjct: 177 GYHVDTRKRLNDSRKERPLERGLIETGKEDDSTLLRSLSMKGLAVTEDQKHGSTYTIKCD 236

Query: 132 VVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLD 191
           VVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFV EDYSSLEGPSMNELYE+GGLKSTLD
Sbjct: 237 VVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVPEDYSSLEGPSMNELYETGGLKSTLD 296

Query: 192 GKVMLLAGTTVGGGSAINWSASIKTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGV 251
           GKVMLLAGTTVGGGSAINWSASI+TPK +L+EWST +KIPL+GS DYQ AMDAVCDRIGV
Sbjct: 297 GKVMLLAGTTVGGGSAINWSASIRTPKPVLEEWSTIHKIPLFGSFDYQSAMDAVCDRIGV 356

Query: 252 TEECPVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVD 311
           TE C +ESFQNQVLRKGCENLGLKVDSV RNSSKDHYCGSCCYGCRVGDKKGTD+TWLVD
Sbjct: 357 TEGCRLESFQNQVLRKGCENLGLKVDSVPRNSSKDHYCGSCCYGCRVGDKKGTDSTWLVD 416

Query: 312 AVEYGAVILTGCQADKFILDNTNNTSKRKRCIGVIATPRSKNITKKLHIEAKATISACGS 371
           AVE GAVILTGC+ADK +L+NTNNTS+ KRC+GVIATP SKN+TKKLHIEAKATISACGS
Sbjct: 417 AVESGAVILTGCRADKLVLENTNNTSRNKRCLGVIATPLSKNVTKKLHIEAKATISACGS 476

Query: 372 LLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSKY 431
           LLTPPLLIASGLRNKNIG+NLHLHPV L WGYFPE+LSDF GKIYEGGLITSLH VVSK 
Sbjct: 477 LLTPPLLIASGLRNKNIGSNLHLHPVILAWGYFPEQLSDFTGKIYEGGLITSLHNVVSKD 536

Query: 432 SNSHAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKANV 491
           SNSHAII+TPA+GPASFAA+FPWTSGR+MKDA+VKY+RTAHLFAL+RD+GAGEIKVKANV
Sbjct: 537 SNSHAIIQTPALGPASFAAMFPWTSGRDMKDAVVKYARTAHLFALVRDRGAGEIKVKANV 596

Query: 492 KYRLNQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVEA 551
           KYRL+QTDKENLRIGLRQALRILIAAGATEVGTYRSDGQR +  GTNKE+LEEF DSVEA
Sbjct: 597 KYRLDQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRFQFHGTNKEDLEEFFDSVEA 656

Query: 552 VGGPLSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAVGV 611
            GGP SR+KYW +YCSAHQMSSCRMGST+EEGAVDENGESWEA+GLFVCDGSVLPTAVGV
Sbjct: 657 AGGPFSREKYWNIYCSAHQMSSCRMGSTEEEGAVDENGESWEAKGLFVCDGSVLPTAVGV 716

Query: 612 NPMITIQSTAYCISKKIAESLN 633
           NPMITIQSTAYCISKK+AESLN
Sbjct: 717 NPMITIQSTAYCISKKLAESLN 738

BLAST of Cucsat.G19362.T1 vs. ExPASy TrEMBL
Match: A0A6J1HG83 (Long-chain-alcohol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111464000 PE=3 SV=1)

HSP 1 Score: 1092 bits (2825), Expect = 0.0
Identity = 534/622 (85.85%), Postives = 581/622 (93.41%), Query Frame = 0

Query: 12  LKFSEISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGM 71
           LKFSEISLEKRE+ILK+WS+AH +YTVLLRM FMIIK+FCCFKFFS ID  +KN+AW+ M
Sbjct: 117 LKFSEISLEKREQILKDWSVAHQRYTVLLRMVFMIIKMFCCFKFFSGIDRESKNIAWEAM 176

Query: 72  GYKVDTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCD 131
           GY+VDTRK+LN T+KERPLERG+IE G EDDSTLL+SLS KGL V ED KHG+ Y IKCD
Sbjct: 177 GYRVDTRKRLNDTRKERPLERGVIEAGKEDDSTLLQSLSMKGLAVTEDPKHGSTYTIKCD 236

Query: 132 VVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLD 191
           VVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFV EDYSSLEGPSMNELYE+GGLKST+D
Sbjct: 237 VVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVPEDYSSLEGPSMNELYETGGLKSTID 296

Query: 192 GKVMLLAGTTVGGGSAINWSASIKTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGV 251
           GKVMLLAGTTVGGGSAINWSASI+TPK +L+EWST +KIPL+GS DY+ AMDAVCDRIGV
Sbjct: 297 GKVMLLAGTTVGGGSAINWSASIRTPKPVLEEWSTIHKIPLFGSFDYRSAMDAVCDRIGV 356

Query: 252 TEECPVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVD 311
           TE C +ESFQNQVLRKGCENLGLKVDSV RNSSKDHYCGSCCYGCRVGDKKGTD TWLVD
Sbjct: 357 TEGCRLESFQNQVLRKGCENLGLKVDSVPRNSSKDHYCGSCCYGCRVGDKKGTDATWLVD 416

Query: 312 AVEYGAVILTGCQADKFILDNTNNTSKRKRCIGVIATPRSKNITKKLHIEAKATISACGS 371
           AVE GAVILTGC+ADK +L+NTNNTS+ KRC+GVIAT  SKN+TKKL+IEAKATISACGS
Sbjct: 417 AVESGAVILTGCRADKLVLENTNNTSRNKRCLGVIATMLSKNVTKKLYIEAKATISACGS 476

Query: 372 LLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSKY 431
           LLTPPLLIASGLRNKNIG+NLHLHPV L WGYFPE+LSDF GKIYEGGLITSLH VVSK 
Sbjct: 477 LLTPPLLIASGLRNKNIGSNLHLHPVILAWGYFPEQLSDFTGKIYEGGLITSLHNVVSKD 536

Query: 432 SNSHAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKANV 491
           SNSHAII+TPA+GPASFAA+FPWTSGR+MKDAMVKY+RTAHLFAL+RD+GAGEIK KANV
Sbjct: 537 SNSHAIIQTPALGPASFAAMFPWTSGRDMKDAMVKYARTAHLFALVRDKGAGEIKAKANV 596

Query: 492 KYRLNQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVEA 551
           KYRL+QTDKENLRIGLRQALRILIAAGATEVGTYRS+GQR R  GTNKE+LEEFLDSVEA
Sbjct: 597 KYRLDQTDKENLRIGLRQALRILIAAGATEVGTYRSNGQRFRFHGTNKEDLEEFLDSVEA 656

Query: 552 VGGPLSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAVGV 611
            GGP SR+KYW +YCSAHQMSSCRMGST+EEGAVDENGESWEA+GLFVCDGSVLPTAVGV
Sbjct: 657 AGGPFSREKYWNIYCSAHQMSSCRMGSTEEEGAVDENGESWEAKGLFVCDGSVLPTAVGV 716

Query: 612 NPMITIQSTAYCISKKIAESLN 633
           NPMITIQSTAYCISKK+AESLN
Sbjct: 717 NPMITIQSTAYCISKKLAESLN 738

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
B5WWZ94.5e-24066.13Long-chain-alcohol oxidase FAO2 OS=Lotus japonicus OX=34305 GN=FAO2 PE=2 SV=1[more]
Q94BP37.1e-22261.15Long-chain-alcohol oxidase FAO4B OS=Arabidopsis thaliana OX=3702 GN=FAO4B PE=2 S... [more]
B5WWZ82.2e-21858.33Long-chain-alcohol oxidase FAO1 OS=Lotus japonicus OX=34305 GN=FAO1 PE=1 SV=1[more]
Q9LW561.3e-21558.57Long-chain-alcohol oxidase FAO3 OS=Arabidopsis thaliana OX=3702 GN=FAO3 PE=1 SV=... [more]
Q9ZWB97.9e-20557.28Long-chain-alcohol oxidase FAO1 OS=Arabidopsis thaliana OX=3702 GN=FAO1 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
XP_004152057.10.0100.00long-chain-alcohol oxidase FAO2 [Cucumis sativus] >KGN58167.1 hypothetical prote... [more]
KAA0037980.10.095.67long-chain-alcohol oxidase FAO2-like [Cucumis melo var. makuwa][more]
XP_008447391.20.095.51PREDICTED: LOW QUALITY PROTEIN: long-chain-alcohol oxidase FAO2-like [Cucumis me... [more]
XP_038888257.10.091.96long-chain-alcohol oxidase FAO2-like [Benincasa hispida][more]
XP_022967423.10.086.66long-chain-alcohol oxidase FAO2-like [Cucurbita maxima] >XP_022967424.1 long-cha... [more]
Match NameE-valueIdentityDescription
A0A0A0LBV70.0100.00Long-chain-alcohol oxidase OS=Cucumis sativus OX=3659 GN=Csa_3G575330 PE=3 SV=1[more]
A0A5A7T3R30.095.67Long-chain-alcohol oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
A0A1S3BHB10.095.51Long-chain-alcohol oxidase OS=Cucumis melo OX=3656 GN=LOC103489850 PE=3 SV=1[more]
A0A6J1HWN70.086.66Long-chain-alcohol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111466966 PE=3 SV=1[more]
A0A6J1HG830.085.85Long-chain-alcohol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111464000 PE=3 SV... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 469..631
e-value: 2.0E-15
score: 58.9
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 115..421
e-value: 4.2E-28
score: 100.3
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 128..632
IPR012400Long-chain-alcohol oxidasePIRSFPIRSF028937Lg_Ch_AOcoord: 3..633
e-value: 2.0E-284
score: 943.4
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPFAMPF00732GMC_oxred_Ncoord: 131..397
e-value: 1.4E-65
score: 221.6
IPR007867Glucose-methanol-choline oxidoreductase, C-terminalPFAMPF05199GMC_oxred_Ccoord: 488..623
e-value: 5.0E-23
score: 82.2
NoneNo IPR availablePANTHERPTHR46056LONG-CHAIN-ALCOHOL OXIDASEcoord: 12..633
NoneNo IPR availablePANTHERPTHR46056:SF12LONG-CHAIN-ALCOHOL OXIDASE FAO4Bcoord: 12..633

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G19362Cucsat.G19362gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G19362.T1.E1Cucsat.G19362.T1.E1exon
Cucsat.G19362.T1.E2Cucsat.G19362.T1.E2exon
Cucsat.G19362.T1.E3Cucsat.G19362.T1.E3exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G19362.T1.C1Cucsat.G19362.T1.C1CDS
Cucsat.G19362.T1.C2Cucsat.G19362.T1.C2CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G19362.T1Cucsat.G19362.T1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0046577 long-chain-alcohol oxidase activity
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors
molecular_function GO:0046593 mandelonitrile lyase activity