Cucsat.G19237.T29 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G19237.T29
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Descriptionmetal-nicotianamine transporter YSL3-like
Locationctg36: 302800 .. 309331 (+)
RNA-Seq ExpressionCucsat.G19237.T29
SyntenyCucsat.G19237.T29
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTTAAAAAAGAAAAAGGAACCCAAAACAACATCCATTCTTAGTTGAGTTTTTGGAAAACACACCTTTGCTTCAGGTTCCAAATTCCAATCACACTCACAAATCTGCTTCAGCTTAAATTTTAAGCTTTTACTCCTTTTTTTTTTTTTCCTTTCTGTTCTCCATCAACAAGAATCTTACCACAATTTCACGTAATTTAGCATCTTAGAACACTCTTCAACCTATCATCTTTATAAGCACCGTATCCTCCTCTAGTTCATCAACTAAATCGTCCCACCCCCCTTGCCTTGCTGCTCCTACAAATCCGTTGCTGCCCTCGCCCCACCATATTCAAATAGGTTCAGTTAGTTTGATTCATTTGTTTATTTGATTTAGCTGTTTGTCTATTGTTTTGAATGGCGAAAGTTTTGGGATTCTATACCCATGCATGAATTATCAATTAGAGATTTGCTGATTGCTATACAATTGTGTGCAGAAATTATCATTATCGTGGTCATATTTTGGATACCATTGAGCAAAACAAGTAAGTTGGTTTCTCATCTATTGTTGTTGTGTCCTCCTAGATTTTAACGCACTAAATGTACTGAAATACGTACTCTCCTTCAATAATTTTCTTACAATCTAGTTAAGTTAACTAGAGAATTAGTTTTACCGTTCAAGTGAGTTTCAGTTAGTTTAACACTAATGCAATAGTACTAAAAATATAAGTAAATTTGAGGTTAAACATGTTTTTAGTTTCTAAACTTTAATTAAAATAACATAATTTAGTTTTTGAATTTAATTAGTTCATATACTTAAATTTTGTTAGAATTTAGAGTTTAGAAATTTTAGTAATTTAGTCAATGTAATTTAATCTCCAATATTAAAAAAAAAAATGGTCAATTTTTATTATTTTATGATATAGAATTTGTATTTTAAAAAAATATTGAGTCTCTAGCTAATAGTTCATTTGGGGAAGAAATCTAATTAAACCTTAATACTAGTTTCTATTGAAAGGAATTGAATGGTTACAAAATTTAAAGTAGAATGTAGCAACTGTTAGGGTTTAATTGTATCAAAATTAAAAATATATGAACAGAAATCAGAATTGTTATAAAAGTAAAGTCATGGGGGAGTTCTTGTTTAAAGTATTTTTTTAACACTGCTTTTTAAACTTTCATGGGGTTGGTTGTAAAAGGGTCCAATACAGTAGGTGAGGGAGGTTGATTGAGGTTTATTTTACAAATAAATAAGTTTTGAATTAGTTTGAAATCCTAAAATTTGGAATTCTTAGAGAGAGCATCCAAACCATATTTACATATTTGGCTTCTAACAATTTGATGCAGAAAAAGAAAAAAAAATATATATTGGGATTCTGATTACTTTTGTACACTTTTAGTGTCTTCAAAAGATATTTCTTATTTTTTTCACTAAATAAGAAATTCTCTTTCTTTCGTTCTCTCTGTTTCCTGTTATGCAAATATAAAATCATTTTAGTTAAAATATTGTTTTGATGATTTAGTTTTAGTTTTTAAAATAGAAAATTGCATTAAATCACATCAAAAATGTAGAAAAAAACACTTCATGATACTTTTTTTTTTCTACTTTTTTGCAAACTCTTCCTTTATAATTATCTAATTTTATTTGTTTGAATTATAATAGATCTTATATCTAATGCATCAAAGTCAAAATTTAATTAAATAATAGTGATTTCCATGCCATCCAAAAAACATAAACTAAATTGTTTTACATCATAAAACTATTCAAAATACATACAAATATAGCAAAATTTTATAATTTATCCATGATAGACATTTATTAGTATCTATCGACAATAAATAATAGTAATATATCAATATCTATCAACTTATATCAGTAATAACAAAAAAGAAAAAATGTAACTAAGGCATAATAAACAAATTTTGGAAAAAAATTTAGATTTATTAAATATATAGGAGCTAAAATTGAACCAAAATAACGATTTAACGAATATTTTTCAACGAGACTTCAAGTTGTATGATTCCCACGTGATGTTGAATCTATCTTGGATAATGTCAAATCTCTACTTTGCATAACCATTGTATTGGTAGAACCTCTATCGTAAATATAACAAAACTTTAAATCTAATCTTGAAAGTTTATTGGTGATAAACTATATAATAGAAATTTTATATATAGTTTAGTTTATGTATGTTAGTGGATAGATTTTGTTATGTTTTAGTTTCATAAAAGTATGAGTGAATATAATAGGAGGCAGTATTATAGAATCTGACTCGTAAATGTTGCTATAGTTGCATTCTTAAAATTATTATTAGCGTTGAAAATGTTTGTTTATGCAATTGCCTCAATACTTTCTTTGTTTGCATATTCACTTTCTATTTATATATTAGAAAAATAAGTTAAAGACTTTTAAAAACTAAAAATATAAATGTGAAGAACTGTTTTGTTCAATAGACATAATTCAAATGTTTTTGGATGGGGAGATAAAGGAAGAAACAAACATAAATTTCATTGGTTTAGAACTAAAAGAAAATGGTTATTAGCACAAGGGCCAACTTTGTAATTTACTGATATTCATCATCCTTTTCTTGTGGGGGTAACAATCAAACAAACAAATCACAAATCTCCCACTCCTTAAATTTCTTCTCTTTATTCTAAAATCTCATCTAATATTAAACTATTTATATAACTCCAATTTCTGGGTTCTTACGCTTGAAACTACGAATATTGTTTTGATATATAGCTGCTTATTCAAATGAGGAACTCCATCATAGAAGAAGTACACGAAATTGAGACTAATGAAAGTAAAGAAGAAGACAAGACTCAAAATGAAGCAGAGGATGTTAAGAAAATTGCACCTTGGACAAGACAGATTACAATCCGTGGTGTAATTGCAAGCATAGCAATAGGAATTATGTACAGTGTAATAGTGATGAAGCTTAATCTTACAACAGGTTTGGTACCAAATTTGAACGTTTCAGTTGCTCTTATTGCATTTGTGTTTATAAAGACATGGACTCTGTTGCTTGAGAAAGCTGGATTTGTATGCACTCCCTTTACACCACAGGAAAACACCGTCATTCAGACTTGTGCTGTTGCTTGTTATAGCATTGCTGTTGGAGGTGAGATAGCTATTGAAATGCTTTGTCGTTTTTTCTGTTTGAAATGTCTTCAATCTGCTGTCTGACACAGTCCACAAGTAGGAGATCTATGGATGTAGTTAACATACGTATACGATTAATGAACCACATAGTCTCTTTGTTCATCTCTCTACTGATCTTTATGTTTTTTGACAGCTGTGTTTGATGATTTTGTGTAGGTGGCTTTGGATCCTACTTGTTTGCTTTGAGCAGGAAGACGTACGAGCAAGCGGGTGTCAACATGGAAGGAAATGCTCCTGGAAGCACCAAAGAACCAGGAATTGGTTGGATCACTGGCTTCCTCTCTGTTAGTAGTTTTGTGGGACTTCTTGCCTTGGTTCCGCTTAGAAAGGTATATTTCTTTCTCATAGAAGTTATGTGTTTACTAAGTGAATCTAAATTTGTTTGTGTACATGTCACACACTCAATGTTATAAACAAACCGAGAAACAAGATGATTAATACAACAGATATAATAAAGACAAATAAACACATAGATTTGTAACTCCATGTGGTGCAAAGTTACTTACGTTTGGGATAGTGTGCTCAGTAGAAAGGAACTTCGCTAATGAAGTTTCACAATTACAATATAGTACTATAGGTAAAAAAATATTTACAAATGACTCACGAATACTCAACTCTTAGTAAAAACTTACGCTATCCATAAGCTTGAGATTCACTGGCTATTCACTGGCTACGAGAAATGCTCTACAGTTGACAGGAGTTTGAGTTCTCCTCAAACTAACTTACATAGGTTGCTCTTATGTATGAGCTCCTCTCAACTTCTCGCCGACCGAGATAAACAATTCAACTTAGGTAACAAAATGCACTCCAAAAACACACAATATGGCCAACCCTAAAAAGGAAGCAACATCTCAATATAGGTTGCTTAAAGACATTCTGAAGGTAAAAAATCATCTCCACGAAACAAAATGCTTTTAAAAGAAATTTCCAATCATCTTGCAGTAACATGATGGAGTTGAGAACTCTAGTTGGTTTTATTAATGGTGTCTTGAATTGTATTGCAGATCATGATACTAGACTATAAATTGACTTATCCAAGTGGAACTGCAACAGCTGTTCTGATTAATGGTTTCCACACACCGAAAGGAGACAAAGCAGCAAAGTATGCAATCCCTTGCTAGTTCTTCTTCATTTAAAACGAAATCGTGTGTTGAACACTTGATTTGAAAGCAACTCTAAAGAAAATTCTTCTATTCATTTCTTTGCCAGGAAACAAGTTCATGGATTCATGAAATACTTTTCATTCAGTTTTTTCTGGGCTCTATTCCAATGGTTTTATTCTGGAGGAGAAAAATGTGGGTTCTCACAGTTTCCAACATTTGGAATAAAAGCTTGGAAAGATTCGTGGGTGCTCAAAATCTTTCCTATTTTCATCTTCTTTGCCATTCTTGAACTTGGTTGGTTTCTTTCATTAAACTGATAGGCATTTATCTTTTGTAGGTTTTACTTTGATTTCAGCTTGACTTATATCGGAGCAGGGATGATATGCCCCCATCTTGTGAACTTGTCATTGCTTCTTGGTGCTGTTCTTTCTTGGGGCGTTATGTGGCCTTTAATGAAGGAACTTAAAGGGGAATGGTATCCTGGATCTCTTCCAGAGAGCAGTATGAAAAGCCTCAATGGTTACAAGGTATAAAATTGGAAACAGGCCATGAAATTTGTAGGGAATTATGTTTTATTTTATTTTGTTAGGTGTGAAATGTCTTAAATTTATTATCTAACCCTTTAAAAAACAAAGCACTGGTTTTCGTTGCTCCGTTAGCAAGTCAAGGTCTAGGGGCACTGCGCTTTAGTGGGACTCAGAGGCAACACTGTCAAAGCCTAATTGTATTCACACACATATATATAGTGTTCGCGATTTTGGCCATGGTTCAGAAGAGTGCGCTATATAAACTTTTTCACTGTGGTGCCACCTCTTAGATCTTTTTATTATTATTTTTTAAACAAAAATAACACTCTTTTTACGATGAAATGAAATCAGATTCATGTCAGTTTTCTTACACATTGTTTCGTGCAGGTATTTATATCAATTGCTTTGATACTTGGAGATGGGCTTTACCATTTCCTCAAAATTCTATATTTCACTGGTTCAAACATGTACGCAAAAGCAACCAACAAGAAGTTGAAAACATGTAAATTGATGATTCCTGATGTGCCATTCCCAAGTTTTCTTTCATTCATTATGACATGATATTTATATGGATGCAGTCCCTGATGATTCAGTCCCAACTTTCGACGATCATCGACGAAACGAAGTGTTCTTAAGAGACGGTATTCCGGTATGGGTGGCAATCACAGGGTACATCTTCTTCTCCATTGTCTCTATCATTGTAATCCCAATCATGTTCTCAGAGGTCAAATGGTACTACATAGTTGTGGCCTATACTTTGGCACCATCTCTTAGCTTTTGCAATGCCTATGGTGCTGGTCTAACTGACATGAATATGGCTTATAACTATGGGAAAGTGGCTCTGTTTGTGCTTGCTGCTATGGCTGGTAAAAATGATGGTGTAGTTGCTGGACTTGTTGGTTGTGGTTTGATCAAGTCTATTGTTTCCATTTCCTCTGATTTGATGCATGATTTCAAAACTGGCCATCTTACACTTACATCTCCAAGATCCATGCTTTTAAGCCAAGCTATTGGTACAGCCTTAGGCTGCATTGTAGCTCCTATCACATTTTATATGTTCTACAAAGCATTTGATCTCGCCAACCCAAATGGTGAGTACAAGGTCCCGTATGCAATCATATACCGAAACATGGCTATCTTAGGAGTTGAAGGCTTCTCAGCTTTGCCTCAGCATTGCTTGCAGCTATGTTATGGATTCTTTAGCTTTGCCATAGTAGCTAACTTGTTGAGAGATCTTACACTTGAGAAATTTGGGAAATGGATCCCATTGCCAATGGTCATGGCTGTGCCTTTCCTCGTCGGTGCTTATTTTGCAATCGATATGTGCATGGGGAGCTTGATCGTGTTCGTGTGGCATTATCTAAATCGTGAAAAGGCTGGATTGATGGTTCCGGCTGTTGCGTCTGGTTTGATATGTGGAGAAGGATTATGGATTCTCCCTTCTTCAATTCTTGCTTTGGCTAAGATTCATCCCCCAATTTGTATGAGTTTTTTTTCTTCTAGTAAAAGTTAAATCTAGAAAGTGCTCTTAAGTATAGAATCTTATGCATGTTCAAGATGTAGAGAAGCTGCAATCTCTTCATAAATTTTAGAATGCAGCAAGTTTCAATATAGTGTTACTGTATAAGTTAAAGGTTAATAGAATACTTTGAGAGATTGAATCAAACTACATCATTAAAACATATTCTTCACCGTAATATTGGATGCATTCAGAAATTTTAAGACGTTAACTCATTGTGGAAACTTTCAGCTACTATGGTA

Coding sequence (CDS)

ATGTACGCAAAAGCAACCAACAAGAAGTTGAAAACATTCCCTGATGATTCAGTCCCAACTTTCGACGATCATCGACGAAACGAAGTGTTCTTAAGAGACGGTATTCCGGTATGGGTGGCAATCACAGGGTACATCTTCTTCTCCATTGTCTCTATCATTGTAATCCCAATCATGTTCTCAGAGGTCAAATGGTACTACATAGTTGTGGCCTATACTTTGGCACCATCTCTTAGCTTTTGCAATGCCTATGGTGCTGGTCTAACTGACATGAATATGGCTTATAACTATGGGAAAGTGGCTCTGTTTGTGCTTGCTGCTATGGCTGGTAAAAATGATGGTGTAGTTGCTGGACTTGTTGGTTGTGGTTTGATCAAGTCTATTGTTTCCATTTCCTCTGATTTGATGCATGATTTCAAAACTGGCCATCTTACACTTACATCTCCAAGATCCATGCTTTTAAGCCAAGCTATTGGTACAGCCTTAGGCTGCATTGTAGCTCCTATCACATTTTATATGTTCTACAAAGCATTTGATCTCGCCAACCCAAATGGTGAGTACAAGGTCCCGTATGCAATCATATACCGAAACATGGCTATCTTAGGAGTTGAAGGCTTCTCAGCTTTGCCTCAGCATTGCTTGCAGCTATGTTATGGATTCTTTAGCTTTGCCATAGTAGCTAACTTGTTGAGAGATCTTACACTTGAGAAATTTGGGAAATGGATCCCATTGCCAATGGTCATGGCTGTGCCTTTCCTCGTCGGTGCTTATTTTGCAATCGATATGTGCATGGGGAGCTTGATCGTGTTCGTGTGGCATTATCTAAATCGTGAAAAGGCTGGATTGATGGTTCCGGCTGTTGCGTCTGGTTTGATATGTGGAGAAGGATTATGGATTCTCCCTTCTTCAATTCTTGCTTTGGCTAAGATTCATCCCCCAATTTGTATGAGTTTTTTTTCTTCTAGTAAAAGTTAA

Protein sequence

MYAKATNKKLKTFPDDSVPTFDDHRRNEVFLRDGIPVWVAITGYIFFSIVSIIVIPIMFSEVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALFVLAAMAGKNDGVVAGLVGCGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLANPNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKWIPLPMVMAVPFLVGAYFAIDMCMGSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILPSSILALAKIHPPICMSFFSSSKS
Homology
BLAST of Cucsat.G19237.T29 vs. ExPASy Swiss-Prot
Match: Q2EF88 (Metal-nicotianamine transporter YSL3 OS=Arabidopsis thaliana OX=3702 GN=YSL3 PE=2 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 4.3e-138
Identity = 241/324 (74.38%), Postives = 275/324 (84.88%), Query Frame = 0

Query: 1   MYAKATNKKL-KTFPDDSVPTFDDHRRNEVFLRDGIPVWVAITGYIFFSIVSIIVIPIMF 60
           MY K  N+   K+  +    +  D +R+E+F+RD IP+WVA  GY  FS+VSII IPIMF
Sbjct: 351 MYVKLNNRNSGKSNSEKDKQSIADLKRDEIFVRDSIPLWVAAVGYAAFSVVSIIAIPIMF 410

Query: 61  SEVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALFVLAAMAGKNDGVVAGLV 120
            E+KWY+IVVAY LAPSL F NAYGAGLTDMNMAYNYGKVALF+LAAMAGK +GVVAGLV
Sbjct: 411 PELKWYFIVVAYMLAPSLGFSNAYGAGLTDMNMAYNYGKVALFILAAMAGKQNGVVAGLV 470

Query: 121 GCGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDL 180
           GCGLIKSIVSISSDLMHDFKTGHLTLTSPRSML+SQAIGTA+GC+VAP+TF++FYKAFD+
Sbjct: 471 GCGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLVSQAIGTAIGCVVAPLTFFLFYKAFDV 530

Query: 181 ANPNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGK 240
            N  GEYK PYA++YRNMAILGVEGFSALPQHCLQLCYGFF+FA+ ANL+RD   +K G 
Sbjct: 531 GNQEGEYKAPYALVYRNMAILGVEGFSALPQHCLQLCYGFFAFAVAANLVRDRLPDKIGN 590

Query: 241 WIPLPMVMAVPFLVGAYFAIDMCMGSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWIL 300
           W+PLPM MAVPFLVG YFAIDMC+GSLIVF W+  +R KAGLMVPAVASGLICG+GLWIL
Sbjct: 591 WVPLPMAMAVPFLVGGYFAIDMCVGSLIVFAWNMRDRVKAGLMVPAVASGLICGDGLWIL 650

Query: 301 PSSILALAKIHPPICMSFFSSSKS 324
           PSS+LALA + PPICM F  S  S
Sbjct: 651 PSSVLALAGVRPPICMGFMPSKYS 674

BLAST of Cucsat.G19237.T29 vs. ExPASy Swiss-Prot
Match: Q6R3K9 (Metal-nicotianamine transporter YSL2 OS=Arabidopsis thaliana OX=3702 GN=YSL2 PE=1 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 5.8e-135
Identity = 228/305 (74.75%), Postives = 273/305 (89.51%), Query Frame = 0

Query: 14  PDDSVPTFDD-HRRNEVFLRDGIPVWVAITGYIFFSIVSIIVIPIMFSEVKWYYIVVAYT 73
           P+DS    D+  R NEVF+R+ IP+W+A  GY+FFS+VSII IP+MF ++KWY+++VAY 
Sbjct: 356 PEDSTKESDNLKRENEVFVRESIPLWMACVGYLFFSLVSIIAIPLMFPQLKWYFVLVAYL 415

Query: 74  LAPSLSFCNAYGAGLTDMNMAYNYGKVALFVLAAMAGKNDGVVAGLVGCGLIKSIVSISS 133
           LAPSLSFCNAYGAGLTDMNMAYNYGK ALFV+AA+AGKNDGVVAG+V CGLIKSIVS+S+
Sbjct: 416 LAPSLSFCNAYGAGLTDMNMAYNYGKAALFVMAALAGKNDGVVAGMVACGLIKSIVSVSA 475

Query: 134 DLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLANPNGEYKVPYAI 193
           DLMHDFKTGHLT TSPRSML++QAIGTA+GC+VAP+TF++FYKAFD+ N NGEYK PYA+
Sbjct: 476 DLMHDFKTGHLTQTSPRSMLVAQAIGTAIGCVVAPLTFFLFYKAFDVGNQNGEYKAPYAM 535

Query: 194 IYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKWIPLPMVMAVPFL 253
           IYRNMAI+GV+G SALP+HCL+LCYGFF+FA+ ANL RDL  +K GKWIPLPM MAVPFL
Sbjct: 536 IYRNMAIIGVQGPSALPKHCLELCYGFFAFAVAANLARDLLPDKPGKWIPLPMAMAVPFL 595

Query: 254 VGAYFAIDMCMGSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILPSSILALAKIHPP 313
           VG  FAIDMC+GSL+V+VW  +NR+KA +MVPAVASGLICG+GLWILPSS+LALAK+ PP
Sbjct: 596 VGGSFAIDMCIGSLVVYVWKKVNRKKADVMVPAVASGLICGDGLWILPSSLLALAKVRPP 655

Query: 314 ICMSF 318
           ICM+F
Sbjct: 656 ICMNF 660

BLAST of Cucsat.G19237.T29 vs. ExPASy Swiss-Prot
Match: Q7XUJ2 (Probable metal-nicotianamine transporter YSL9 OS=Oryza sativa subsp. japonica OX=39947 GN=YSL9 PE=2 SV=2)

HSP 1 Score: 470.3 bits (1209), Expect = 1.7e-131
Identity = 214/306 (69.93%), Postives = 265/306 (86.60%), Query Frame = 0

Query: 16  DSVPTFDDHRRNEVFLRDGIPVWVAITGYIFFSIVSIIVIPIMFSEVKWYYIVVAYTLAP 75
           + +P  D+  RNEVF  D IP W+A +GY+  + +++I IP+MF E+KWYY+V+AY LAP
Sbjct: 379 EDMPVLDELHRNEVFTTDNIPSWLAFSGYLGLTFIAVIAIPMMFHEMKWYYVVIAYLLAP 438

Query: 76  SLSFCNAYGAGLTDMNMAYNYGKVALFVLAAMAGKNDGVVAGLVGCGLIKSIVSISSDLM 135
           +L FCNAYGAGLTD+NMAYNYGK+ALF+LAA AGK+ GVVAGLVGCGL+KS+VSIS+DLM
Sbjct: 439 ALGFCNAYGAGLTDINMAYNYGKIALFILAAWAGKDSGVVAGLVGCGLVKSLVSISADLM 498

Query: 136 HDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLANPNGEYKVPYAIIYR 195
           HDFKTGHLTLTSPRSM+++QAIGT +GC+++P+TF++FY AFD+ NP G +K PYA++YR
Sbjct: 499 HDFKTGHLTLTSPRSMIIAQAIGTVMGCVISPLTFFLFYSAFDIGNPEGYWKAPYALVYR 558

Query: 196 NMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKWIPLPMVMAVPFLVGA 255
           NMAILGVEGFSALPQHCLQLCYGFF FA+ ANL RDL   K+G+W+PLPM M VPFLVGA
Sbjct: 559 NMAILGVEGFSALPQHCLQLCYGFFGFAVAANLTRDLCPPKYGRWVPLPMAMGVPFLVGA 618

Query: 256 YFAIDMCMGSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILPSSILALAKIHPPICM 315
            FAIDMC+GSLIVF WH +++ KA LMVPAVASGLICG+GLWI P+S+LALAKI PP+CM
Sbjct: 619 SFAIDMCIGSLIVFTWHIIDKSKAALMVPAVASGLICGDGLWIFPASLLALAKISPPMCM 678

Query: 316 SFFSSS 322
           +F S++
Sbjct: 679 AFRSTN 684

BLAST of Cucsat.G19237.T29 vs. ExPASy Swiss-Prot
Match: Q7XN54 (Probable metal-nicotianamine transporter YSL16 OS=Oryza sativa subsp. japonica OX=39947 GN=YSL16 PE=2 SV=2)

HSP 1 Score: 455.7 bits (1171), Expect = 4.4e-127
Identity = 206/303 (67.99%), Postives = 257/303 (84.82%), Query Frame = 0

Query: 15  DDSVPTFDDHRRNEVFLRDGIPVWVAITGYIFFSIVSIIVIPIMFSEVKWYYIVVAYTLA 74
           D      DD +RNEVF RD IP W+A TGY   S++++++IP+MF +VKWYY+++AY LA
Sbjct: 369 DADTMAIDDMQRNEVFNRDNIPTWMAYTGYTLLSVIAVVLIPVMFRQVKWYYVIIAYLLA 428

Query: 75  PSLSFCNAYGAGLTDMNMAYNYGKVALFVLAAMAGKNDGVVAGLVGCGLIKSIVSISSDL 134
           P+L FCNAYG GLTDMNM YNYGK+ALF+ AA AGK+DGVVAGLVGCGL+K +V IS+DL
Sbjct: 429 PALGFCNAYGTGLTDMNMGYNYGKIALFIFAAWAGKDDGVVAGLVGCGLVKQLVLISADL 488

Query: 135 MHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLANPNGEYKVPYAIIY 194
           MHDFKTGHLTLTSPRSML+ Q +GT +GC+VAP+TF++FYKAFD+ +PNG +K PYA+IY
Sbjct: 489 MHDFKTGHLTLTSPRSMLVGQVVGTLMGCVVAPLTFFLFYKAFDVGDPNGYWKAPYALIY 548

Query: 195 RNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKWIPLPMVMAVPFLVG 254
           RNMAI+GVEGFSALP+HCLQLC GFF+FA++ANL RD    ++G+++PLPM MAVPFLVG
Sbjct: 549 RNMAIIGVEGFSALPRHCLQLCAGFFAFAVLANLARDFLPRRYGRYMPLPMAMAVPFLVG 608

Query: 255 AYFAIDMCMGSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILPSSILALAKIHPPIC 314
           A FAIDMC GSL+VF+WH  + ++A L+VPAVASGLICG+G+W  PSS+LALAK+ PPIC
Sbjct: 609 ASFAIDMCAGSLVVFLWHRFDGKRAALLVPAVASGLICGDGIWTFPSSLLALAKVKPPIC 668

Query: 315 MSF 318
           M F
Sbjct: 669 MKF 671

BLAST of Cucsat.G19237.T29 vs. ExPASy Swiss-Prot
Match: Q6R3L0 (Metal-nicotianamine transporter YSL1 OS=Arabidopsis thaliana OX=3702 GN=YSL1 PE=2 SV=2)

HSP 1 Score: 439.9 bits (1130), Expect = 2.5e-122
Identity = 206/319 (64.58%), Postives = 261/319 (81.82%), Query Frame = 0

Query: 7   NKKLKTFPDD-----SVPTFDDHRRNEVFLRDGIPVWVAITGYIFFSIVSIIVIPIMFSE 66
           N +LK  P+D           D + +E FLRD IP+W A++GY+ F+ VS +V+P++F +
Sbjct: 355 NARLKNKPNDLDDVGHKKQRKDLKEDENFLRDKIPMWFAVSGYLTFAAVSTVVVPLIFPQ 414

Query: 67  VKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALFVLAAMAGKNDGVVAGLVGC 126
           +KWYY++VAY  APSL+FCNAYGAGLTD+NMAYNYGK+ LFV+AA+ G+ +GVVAGL GC
Sbjct: 415 LKWYYVIVAYIFAPSLAFCNAYGAGLTDINMAYNYGKIGLFVIAAVTGRENGVVAGLAGC 474

Query: 127 GLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLAN 186
           GLIKS+VS+S  LM DFKT H T+TSP++M  SQ IGT +GCIV P++F++FYKAFD+ N
Sbjct: 475 GLIKSVVSVSCILMQDFKTAHYTMTSPKAMFASQMIGTVVGCIVTPLSFFLFYKAFDIGN 534

Query: 187 PNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKWI 246
           PNGE+K PYA+IYRNMAILGV+GFSALP HCLQ+CYGFF FA++ N++RDLT  K G+++
Sbjct: 535 PNGEFKAPYALIYRNMAILGVQGFSALPLHCLQMCYGFFGFAVLVNVVRDLTPAKIGRFM 594

Query: 247 PLPMVMAVPFLVGAYFAIDMCMGSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILPS 306
           PLP  MAVPFLVGAYFAIDMC+G+LIVFVW  +NR+KA  MVPAVASGLICGEGLW LP+
Sbjct: 595 PLPTAMAVPFLVGAYFAIDMCVGTLIVFVWEKMNRKKAEFMVPAVASGLICGEGLWTLPA 654

Query: 307 SILALAKIHPPICMSFFSS 321
           ++LALA + PPICM F +S
Sbjct: 655 AVLALAGVKPPICMKFLAS 673

BLAST of Cucsat.G19237.T29 vs. NCBI nr
Match: XP_031736196.1 (metal-nicotianamine transporter YSL3 isoform X3 [Cucumis sativus])

HSP 1 Score: 642 bits (1656), Expect = 5.55e-229
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0

Query: 1   MYAKATNKKLKTFPDDSVPTFDDHRRNEVFLRDGIPVWVAITGYIFFSIVSIIVIPIMFS 60
           MYAKATNKKLKTFPDDSVPTFDDHRRNEVFLRDGIPVWVAITGYIFFSIVSIIVIPIMFS
Sbjct: 205 MYAKATNKKLKTFPDDSVPTFDDHRRNEVFLRDGIPVWVAITGYIFFSIVSIIVIPIMFS 264

Query: 61  EVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALFVLAAMAGKNDGVVAGLVG 120
           EVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALFVLAAMAGKNDGVVAGLVG
Sbjct: 265 EVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALFVLAAMAGKNDGVVAGLVG 324

Query: 121 CGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLA 180
           CGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLA
Sbjct: 325 CGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLA 384

Query: 181 NPNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKW 240
           NPNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKW
Sbjct: 385 NPNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKW 444

Query: 241 IPLPMVMAVPFLVGAYFAIDMCMGSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILP 300
           IPLPMVMAVPFLVGAYFAIDMCMGSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILP
Sbjct: 445 IPLPMVMAVPFLVGAYFAIDMCMGSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILP 504

Query: 301 SSILALAKIHPPICMSFFSSSKS 323
           SSILALAKIHPPICMSFFSSSKS
Sbjct: 505 SSILALAKIHPPICMSFFSSSKS 527

BLAST of Cucsat.G19237.T29 vs. NCBI nr
Match: XP_031736195.1 (metal-nicotianamine transporter YSL3 isoform X2 [Cucumis sativus] >KAE8653095.1 hypothetical protein Csa_019934 [Cucumis sativus])

HSP 1 Score: 642 bits (1656), Expect = 3.24e-227
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0

Query: 1   MYAKATNKKLKTFPDDSVPTFDDHRRNEVFLRDGIPVWVAITGYIFFSIVSIIVIPIMFS 60
           MYAKATNKKLKTFPDDSVPTFDDHRRNEVFLRDGIPVWVAITGYIFFSIVSIIVIPIMFS
Sbjct: 321 MYAKATNKKLKTFPDDSVPTFDDHRRNEVFLRDGIPVWVAITGYIFFSIVSIIVIPIMFS 380

Query: 61  EVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALFVLAAMAGKNDGVVAGLVG 120
           EVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALFVLAAMAGKNDGVVAGLVG
Sbjct: 381 EVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALFVLAAMAGKNDGVVAGLVG 440

Query: 121 CGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLA 180
           CGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLA
Sbjct: 441 CGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLA 500

Query: 181 NPNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKW 240
           NPNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKW
Sbjct: 501 NPNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKW 560

Query: 241 IPLPMVMAVPFLVGAYFAIDMCMGSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILP 300
           IPLPMVMAVPFLVGAYFAIDMCMGSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILP
Sbjct: 561 IPLPMVMAVPFLVGAYFAIDMCMGSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILP 620

Query: 301 SSILALAKIHPPICMSFFSSSKS 323
           SSILALAKIHPPICMSFFSSSKS
Sbjct: 621 SSILALAKIHPPICMSFFSSSKS 643

BLAST of Cucsat.G19237.T29 vs. NCBI nr
Match: XP_004150025.2 (metal-nicotianamine transporter YSL3 isoform X1 [Cucumis sativus])

HSP 1 Score: 642 bits (1656), Expect = 8.17e-227
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0

Query: 1   MYAKATNKKLKTFPDDSVPTFDDHRRNEVFLRDGIPVWVAITGYIFFSIVSIIVIPIMFS 60
           MYAKATNKKLKTFPDDSVPTFDDHRRNEVFLRDGIPVWVAITGYIFFSIVSIIVIPIMFS
Sbjct: 348 MYAKATNKKLKTFPDDSVPTFDDHRRNEVFLRDGIPVWVAITGYIFFSIVSIIVIPIMFS 407

Query: 61  EVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALFVLAAMAGKNDGVVAGLVG 120
           EVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALFVLAAMAGKNDGVVAGLVG
Sbjct: 408 EVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALFVLAAMAGKNDGVVAGLVG 467

Query: 121 CGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLA 180
           CGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLA
Sbjct: 468 CGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLA 527

Query: 181 NPNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKW 240
           NPNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKW
Sbjct: 528 NPNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKW 587

Query: 241 IPLPMVMAVPFLVGAYFAIDMCMGSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILP 300
           IPLPMVMAVPFLVGAYFAIDMCMGSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILP
Sbjct: 588 IPLPMVMAVPFLVGAYFAIDMCMGSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILP 647

Query: 301 SSILALAKIHPPICMSFFSSSKS 323
           SSILALAKIHPPICMSFFSSSKS
Sbjct: 648 SSILALAKIHPPICMSFFSSSKS 670

BLAST of Cucsat.G19237.T29 vs. NCBI nr
Match: KAA0046808.1 (metal-nicotianamine transporter YSL3-like isoform X1 [Cucumis melo var. makuwa] >TYK26940.1 metal-nicotianamine transporter YSL3-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 630 bits (1624), Expect = 5.90e-222
Identity = 317/323 (98.14%), Postives = 320/323 (99.07%), Query Frame = 0

Query: 1   MYAKATNKKLKTFPDDSVPTFDDHRRNEVFLRDGIPVWVAITGYIFFSIVSIIVIPIMFS 60
           MYAKATNKKLKTFPDDSV TF+DHRR EVFLRD IPVWVAITGYIFFSIVSIIVIPIMFS
Sbjct: 348 MYAKATNKKLKTFPDDSVQTFNDHRRKEVFLRDSIPVWVAITGYIFFSIVSIIVIPIMFS 407

Query: 61  EVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALFVLAAMAGKNDGVVAGLVG 120
           EVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALF+LAAMAGKNDGVVAGLVG
Sbjct: 408 EVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALFLLAAMAGKNDGVVAGLVG 467

Query: 121 CGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLA 180
           CGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLA
Sbjct: 468 CGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLA 527

Query: 181 NPNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKW 240
           NPNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKW
Sbjct: 528 NPNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKW 587

Query: 241 IPLPMVMAVPFLVGAYFAIDMCMGSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILP 300
           IPLPMVMAVPFLVGAYFAIDMC+GSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILP
Sbjct: 588 IPLPMVMAVPFLVGAYFAIDMCVGSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILP 647

Query: 301 SSILALAKIHPPICMSFFSSSKS 323
           SSILALAKIHPPICMSFFSSSKS
Sbjct: 648 SSILALAKIHPPICMSFFSSSKS 670

BLAST of Cucsat.G19237.T29 vs. NCBI nr
Match: XP_016899827.1 (PREDICTED: metal-nicotianamine transporter YSL3-like isoform X2 [Cucumis melo] >XP_016899828.1 PREDICTED: metal-nicotianamine transporter YSL3-like isoform X2 [Cucumis melo])

HSP 1 Score: 622 bits (1605), Expect = 1.22e-220
Identity = 312/318 (98.11%), Postives = 315/318 (99.06%), Query Frame = 0

Query: 1   MYAKATNKKLKTFPDDSVPTFDDHRRNEVFLRDGIPVWVAITGYIFFSIVSIIVIPIMFS 60
           MYAKATNKKLKTFPDDSV TF+DHRR EVFLRD IPVWVAITGYIFFSIVSIIVIPIMFS
Sbjct: 209 MYAKATNKKLKTFPDDSVQTFNDHRRKEVFLRDSIPVWVAITGYIFFSIVSIIVIPIMFS 268

Query: 61  EVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALFVLAAMAGKNDGVVAGLVG 120
           EVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALF+LAAMAGKNDGVVAGLVG
Sbjct: 269 EVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALFLLAAMAGKNDGVVAGLVG 328

Query: 121 CGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLA 180
           CGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLA
Sbjct: 329 CGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLA 388

Query: 181 NPNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKW 240
           NPNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKW
Sbjct: 389 NPNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKW 448

Query: 241 IPLPMVMAVPFLVGAYFAIDMCMGSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILP 300
           IPLPMVMAVPFLVGAYFAIDMC+GSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILP
Sbjct: 449 IPLPMVMAVPFLVGAYFAIDMCVGSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILP 508

Query: 301 SSILALAKIHPPICMSFF 318
           SSILALAKIHPPICMSFF
Sbjct: 509 SSILALAKIHPPICMSFF 526

BLAST of Cucsat.G19237.T29 vs. ExPASy TrEMBL
Match: A0A0A0LWG3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G329900 PE=4 SV=1)

HSP 1 Score: 642 bits (1656), Expect = 6.39e-228
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0

Query: 1   MYAKATNKKLKTFPDDSVPTFDDHRRNEVFLRDGIPVWVAITGYIFFSIVSIIVIPIMFS 60
           MYAKATNKKLKTFPDDSVPTFDDHRRNEVFLRDGIPVWVAITGYIFFSIVSIIVIPIMFS
Sbjct: 295 MYAKATNKKLKTFPDDSVPTFDDHRRNEVFLRDGIPVWVAITGYIFFSIVSIIVIPIMFS 354

Query: 61  EVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALFVLAAMAGKNDGVVAGLVG 120
           EVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALFVLAAMAGKNDGVVAGLVG
Sbjct: 355 EVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALFVLAAMAGKNDGVVAGLVG 414

Query: 121 CGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLA 180
           CGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLA
Sbjct: 415 CGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLA 474

Query: 181 NPNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKW 240
           NPNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKW
Sbjct: 475 NPNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKW 534

Query: 241 IPLPMVMAVPFLVGAYFAIDMCMGSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILP 300
           IPLPMVMAVPFLVGAYFAIDMCMGSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILP
Sbjct: 535 IPLPMVMAVPFLVGAYFAIDMCMGSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILP 594

Query: 301 SSILALAKIHPPICMSFFSSSKS 323
           SSILALAKIHPPICMSFFSSSKS
Sbjct: 595 SSILALAKIHPPICMSFFSSSKS 617

BLAST of Cucsat.G19237.T29 vs. ExPASy TrEMBL
Match: A0A5A7TVA7 (Metal-nicotianamine transporter YSL3-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold876G00230 PE=4 SV=1)

HSP 1 Score: 630 bits (1624), Expect = 2.86e-222
Identity = 317/323 (98.14%), Postives = 320/323 (99.07%), Query Frame = 0

Query: 1   MYAKATNKKLKTFPDDSVPTFDDHRRNEVFLRDGIPVWVAITGYIFFSIVSIIVIPIMFS 60
           MYAKATNKKLKTFPDDSV TF+DHRR EVFLRD IPVWVAITGYIFFSIVSIIVIPIMFS
Sbjct: 348 MYAKATNKKLKTFPDDSVQTFNDHRRKEVFLRDSIPVWVAITGYIFFSIVSIIVIPIMFS 407

Query: 61  EVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALFVLAAMAGKNDGVVAGLVG 120
           EVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALF+LAAMAGKNDGVVAGLVG
Sbjct: 408 EVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALFLLAAMAGKNDGVVAGLVG 467

Query: 121 CGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLA 180
           CGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLA
Sbjct: 468 CGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLA 527

Query: 181 NPNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKW 240
           NPNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKW
Sbjct: 528 NPNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKW 587

Query: 241 IPLPMVMAVPFLVGAYFAIDMCMGSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILP 300
           IPLPMVMAVPFLVGAYFAIDMC+GSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILP
Sbjct: 588 IPLPMVMAVPFLVGAYFAIDMCVGSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILP 647

Query: 301 SSILALAKIHPPICMSFFSSSKS 323
           SSILALAKIHPPICMSFFSSSKS
Sbjct: 648 SSILALAKIHPPICMSFFSSSKS 670

BLAST of Cucsat.G19237.T29 vs. ExPASy TrEMBL
Match: A0A1S4DV12 (metal-nicotianamine transporter YSL3-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103487454 PE=4 SV=1)

HSP 1 Score: 622 bits (1605), Expect = 5.89e-221
Identity = 312/318 (98.11%), Postives = 315/318 (99.06%), Query Frame = 0

Query: 1   MYAKATNKKLKTFPDDSVPTFDDHRRNEVFLRDGIPVWVAITGYIFFSIVSIIVIPIMFS 60
           MYAKATNKKLKTFPDDSV TF+DHRR EVFLRD IPVWVAITGYIFFSIVSIIVIPIMFS
Sbjct: 209 MYAKATNKKLKTFPDDSVQTFNDHRRKEVFLRDSIPVWVAITGYIFFSIVSIIVIPIMFS 268

Query: 61  EVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALFVLAAMAGKNDGVVAGLVG 120
           EVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALF+LAAMAGKNDGVVAGLVG
Sbjct: 269 EVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALFLLAAMAGKNDGVVAGLVG 328

Query: 121 CGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLA 180
           CGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLA
Sbjct: 329 CGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLA 388

Query: 181 NPNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKW 240
           NPNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKW
Sbjct: 389 NPNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKW 448

Query: 241 IPLPMVMAVPFLVGAYFAIDMCMGSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILP 300
           IPLPMVMAVPFLVGAYFAIDMC+GSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILP
Sbjct: 449 IPLPMVMAVPFLVGAYFAIDMCVGSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILP 508

Query: 301 SSILALAKIHPPICMSFF 318
           SSILALAKIHPPICMSFF
Sbjct: 509 SSILALAKIHPPICMSFF 526

BLAST of Cucsat.G19237.T29 vs. ExPASy TrEMBL
Match: A0A1S3BA47 (metal-nicotianamine transporter YSL3-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487454 PE=4 SV=1)

HSP 1 Score: 622 bits (1605), Expect = 6.85e-219
Identity = 312/318 (98.11%), Postives = 315/318 (99.06%), Query Frame = 0

Query: 1   MYAKATNKKLKTFPDDSVPTFDDHRRNEVFLRDGIPVWVAITGYIFFSIVSIIVIPIMFS 60
           MYAKATNKKLKTFPDDSV TF+DHRR EVFLRD IPVWVAITGYIFFSIVSIIVIPIMFS
Sbjct: 348 MYAKATNKKLKTFPDDSVQTFNDHRRKEVFLRDSIPVWVAITGYIFFSIVSIIVIPIMFS 407

Query: 61  EVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALFVLAAMAGKNDGVVAGLVG 120
           EVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALF+LAAMAGKNDGVVAGLVG
Sbjct: 408 EVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALFLLAAMAGKNDGVVAGLVG 467

Query: 121 CGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLA 180
           CGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLA
Sbjct: 468 CGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLA 527

Query: 181 NPNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKW 240
           NPNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKW
Sbjct: 528 NPNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKW 587

Query: 241 IPLPMVMAVPFLVGAYFAIDMCMGSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILP 300
           IPLPMVMAVPFLVGAYFAIDMC+GSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILP
Sbjct: 588 IPLPMVMAVPFLVGAYFAIDMCVGSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILP 647

Query: 301 SSILALAKIHPPICMSFF 318
           SSILALAKIHPPICMSFF
Sbjct: 648 SSILALAKIHPPICMSFF 665

BLAST of Cucsat.G19237.T29 vs. ExPASy TrEMBL
Match: A0A1S3C1T1 (metal-nicotianamine transporter YSL3 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103496061 PE=4 SV=1)

HSP 1 Score: 607 bits (1566), Expect = 4.66e-215
Identity = 298/320 (93.12%), Postives = 312/320 (97.50%), Query Frame = 0

Query: 1   MYAKATNKKLKTFPDDSVPTFDDHRRNEVFLRDGIPVWVAITGYIFFSIVSIIVIPIMFS 60
           MYAKATNKKLKTFPDDS+  FDDHRRNEVFLRDGIP+WVA+ GYIFFSIVSI+VIPIMF 
Sbjct: 242 MYAKATNKKLKTFPDDSIQAFDDHRRNEVFLRDGIPIWVAVIGYIFFSIVSIVVIPIMFP 301

Query: 61  EVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALFVLAAMAGKNDGVVAGLVG 120
           EVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALFVLAAMAGKNDGVVAGLVG
Sbjct: 302 EVKWYYIVVAYTLAPSLSFCNAYGAGLTDMNMAYNYGKVALFVLAAMAGKNDGVVAGLVG 361

Query: 121 CGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLSQAIGTALGCIVAPITFYMFYKAFDLA 180
           CGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLL QAIGTA+GCIVAP+TFYMFYKAFDLA
Sbjct: 362 CGLIKSIVSISSDLMHDFKTGHLTLTSPRSMLLGQAIGTAIGCIVAPVTFYMFYKAFDLA 421

Query: 181 NPNGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTLEKFGKW 240
           NP+GEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLT +KFGKW
Sbjct: 422 NPDGEYKVPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAIVANLLRDLTPKKFGKW 481

Query: 241 IPLPMVMAVPFLVGAYFAIDMCMGSLIVFVWHYLNREKAGLMVPAVASGLICGEGLWILP 300
           IPLPM MAVPFLVGAYFAIDMC+GSLIVFVWHYLNR++A LM+PAVASGLICGEGLWILP
Sbjct: 482 IPLPMAMAVPFLVGAYFAIDMCVGSLIVFVWHYLNRQRAELMIPAVASGLICGEGLWILP 541

Query: 301 SSILALAKIHPPICMSFFSS 320
           SSILALAK+HPPICM+FFSS
Sbjct: 542 SSILALAKVHPPICMNFFSS 561

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q2EF884.3e-13874.38Metal-nicotianamine transporter YSL3 OS=Arabidopsis thaliana OX=3702 GN=YSL3 PE=... [more]
Q6R3K95.8e-13574.75Metal-nicotianamine transporter YSL2 OS=Arabidopsis thaliana OX=3702 GN=YSL2 PE=... [more]
Q7XUJ21.7e-13169.93Probable metal-nicotianamine transporter YSL9 OS=Oryza sativa subsp. japonica OX... [more]
Q7XN544.4e-12767.99Probable metal-nicotianamine transporter YSL16 OS=Oryza sativa subsp. japonica O... [more]
Q6R3L02.5e-12264.58Metal-nicotianamine transporter YSL1 OS=Arabidopsis thaliana OX=3702 GN=YSL1 PE=... [more]
Match NameE-valueIdentityDescription
XP_031736196.15.55e-229100.00metal-nicotianamine transporter YSL3 isoform X3 [Cucumis sativus][more]
XP_031736195.13.24e-227100.00metal-nicotianamine transporter YSL3 isoform X2 [Cucumis sativus] >KAE8653095.1 ... [more]
XP_004150025.28.17e-227100.00metal-nicotianamine transporter YSL3 isoform X1 [Cucumis sativus][more]
KAA0046808.15.90e-22298.14metal-nicotianamine transporter YSL3-like isoform X1 [Cucumis melo var. makuwa] ... [more]
XP_016899827.11.22e-22098.11PREDICTED: metal-nicotianamine transporter YSL3-like isoform X2 [Cucumis melo] >... [more]
Match NameE-valueIdentityDescription
A0A0A0LWG36.39e-228100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G329900 PE=4 SV=1[more]
A0A5A7TVA72.86e-22298.14Metal-nicotianamine transporter YSL3-like isoform X1 OS=Cucumis melo var. makuwa... [more]
A0A1S4DV125.89e-22198.11metal-nicotianamine transporter YSL3-like isoform X2 OS=Cucumis melo OX=3656 GN=... [more]
A0A1S3BA476.85e-21998.11metal-nicotianamine transporter YSL3-like isoform X1 OS=Cucumis melo OX=3656 GN=... [more]
A0A1S3C1T14.66e-21593.13metal-nicotianamine transporter YSL3 isoform X3 OS=Cucumis melo OX=3656 GN=LOC10... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004813Oligopeptide transporter, OPT superfamilyPFAMPF03169OPTcoord: 4..304
e-value: 2.9E-64
score: 217.8
IPR004813Oligopeptide transporter, OPT superfamilyTIGRFAMTIGR00728TIGR00728coord: 9..303
e-value: 2.4E-60
score: 202.8
IPR045035Metal-nicotianamine transporter YSL-likePANTHERPTHR31645OLIGOPEPTIDE TRANSPORTER YGL114W-RELATEDcoord: 4..319
NoneNo IPR availablePANTHERPTHR31645:SF4METAL-NICOTIANAMINE TRANSPORTER YSL3coord: 4..319

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G19237Cucsat.G19237gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G19237.T29.E1Cucsat.G19237.T29.E1exon
Cucsat.G19237.T29.E2Cucsat.G19237.T29.E2exon
Cucsat.G19237.T29.E3Cucsat.G19237.T29.E3exon
Cucsat.G19237.T29.E4Cucsat.G19237.T29.E4exon
Cucsat.G19237.T29.E5Cucsat.G19237.T29.E5exon
Cucsat.G19237.T29.E6Cucsat.G19237.T29.E6exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G19237.T29.C1Cucsat.G19237.T29.C1CDS
Cucsat.G19237.T29.C2Cucsat.G19237.T29.C2CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G19237.T29Cucsat.G19237.T29-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035672 oligopeptide transmembrane transport
biological_process GO:0010039 response to iron ion
biological_process GO:0048316 seed development
biological_process GO:0033214 siderophore-dependent iron import into cell
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0035673 oligopeptide transmembrane transporter activity
molecular_function GO:0051980 iron-nicotianamine transmembrane transporter activity