Cucsat.G18713.T3 (mRNA) Cucumber (B10) v3

BLAST of Cucsat.G18713.T3 vs. ExPASy Swiss-Prot
Match: O15050 (TPR and ankyrin repeat-containing protein 1 OS=Homo sapiens OX=9606 GN=TRANK1 PE=2 SV=4)

HSP 1 Score: 199.5 bits (506), Expect = 2.9e-49
Identity = 199/795 (25.03%), Postives = 355/795 (44.65%), Query Frame = 0

Query: 437  LRQLFITVTLKQCLDVKERLAYLKRISNGGNVFKETQNLCKADVLDMNDVQDLLDVPNSF 496
            L Q+F+T     C +V+     L + +   + +K           +++ +QDL D     
Sbjct: 1146 LHQIFVTKNHVLCQEVQRNFIELSKSTKATSHYKPLDP-------NIHKLQDLRD----- 1205

Query: 497  DGIPANSFPLVITFRKFLIMLDRTVGDSYFIRFQ----KQWRLSGGKPKDSL-------- 556
                  +FPL +T ++ L++LD ++   +F+R +    K+  +     ++S         
Sbjct: 1206 -----ENFPLFVTSKQLLLLLDASLPKPFFLRNEDGSLKRTIIGWSAQEESTIPSWQEDE 1265

Query: 557  SRAAYNFIVSKE----------------VTVKNFASSYWSYFDSCLTYKLDAVVVFNEII 616
              A  +   S+E                VT + F +  W       T   +  +++ EI 
Sbjct: 1266 EEAEVDGDYSEEDKAVEMRTGDSDPRVYVTFEVFKNEIWPKMTKGRT-AYNPALIWKEIK 1325

Query: 617  SQIKGGLGAKDALDGRLSKLDYTRLSKGQSTLSRKQRERIYDIFLDYEKMKNAKGEYDLA 676
            S +KG   A     GRL++  Y +L + +    ++ R  IY +F  Y+++++ KG +D  
Sbjct: 1326 SFLKGSFEALSCPHGRLTEEVYKKLGRKRCPNFKEDRSEIYSLFSLYQQIRSQKGYFDEE 1385

Query: 677  DLVIDLHRRLKVFQYTGDHMDFVYVDEVQALTMMQITLLKYLCKNVNSGFVFSSNTTQTI 736
            D++ ++ RRL   +     +  +Y DE+Q  T  ++ LL     + NS F+ + +T Q+I
Sbjct: 1386 DVLYNISRRLSKLRVLPWSIHELYGDEIQDFTQAELALLMKCINDPNSMFL-TGDTAQSI 1445

Query: 737  AKGIDFRFQDIRFLFYKEFISGVKTDEKGIDAGLIKIPDILHINQNCRTQPKILQLANSV 796
             KG+ FRF D+R LF+  + S    D++     + K   I  + QN R+   IL LA+ V
Sbjct: 1446 MKGVAFRFSDLRSLFH--YASRNTIDKQ---CAVRKPKKIHQLYQNYRSHSGILNLASGV 1505

Query: 797  TDLLFRFFPRCIDIVCPETSEMSSTDFETPVLLESRKGQNMMMVLFEEGRNIPADARGYG 856
             DLL  +FP   D + P  S +   D   P +LES    ++ ++L   G         +G
Sbjct: 1506 VDLLQFYFPESFDRL-PRDSGL--FDGPKPTVLESCSVSDLAILL--RGNKRKTQPIEFG 1565

Query: 857  AKQVILVRDECARDEISSLVGNQAIIVTIMECQCMEFQDVLLYKFFNSSPLGNQWRVIYQ 916
            A QVILV +E A+++I   +G  A+++TI E + +EF DVLLY FF  S    +W++I  
Sbjct: 1566 AHQVILVANETAKEKIPEELG-LALVLTIYEAKGLEFDDVLLYNFFTDSEAYKEWKIISS 1625

Query: 917  YM---------------IEQDMLEIAPGGSPSFNQPVQLDLCWELKLLHIALTRSRRRLW 976
            +                +  D    + G S   N  +   L  ELK L+ A+TR+R  LW
Sbjct: 1626 FTPTSTDSREENRPLVEVPLDKPGSSQGRSLMVNPEMYKLLNGELKQLYTAITRARVNLW 1685

Query: 977  IYEDNQEFSNPIVDYWKKLCYVQV----KTLDYSIAQTMKVPSTKEEWSSLGLEFFSEGV 1036
            I+++N+E   P   Y+ +  +VQV    +  D+  +  +K  ST  EW + G  +     
Sbjct: 1686 IFDENREKRAPAFKYFIRRDFVQVVKTDENKDFDDSMFVKT-STPAEWIAQGDYYAKHQC 1745

Query: 1037 YGAASLCFERAEDRRRSEWARAASFCAT-----ANPQISRNALREAAEIYISLDRAEIAA 1096
            +  A+ C+++     + + A A     +      +P+  +    E A+ Y+      ++ 
Sbjct: 1746 WKVAAKCYQKGGAFEKEKLALAHDTALSMKSKKVSPKEKQLEYLELAKTYLECKEPTLSL 1805

Query: 1097 KCYIELKEYKTAAYTYLTKCGEARLEDAGDCYMLAKCYKLAAVAYSMGRCFLKFFDVCTA 1156
            KC    KE++ +A     + G+ R  DA   Y  ++CYK A       RCF +  +   A
Sbjct: 1806 KCLSYAKEFQLSA-QLCERLGKIR--DAAYFYKRSQCYKDAF------RCFEQIQEFDLA 1865

Query: 1157 ANLF---DTGLQGICSWRKYDNVDLIKKCKHIKEAWHL--FLWKGALHYHQLQNFGSMMR 1175
              ++   +   +   +  KY+ +   K     K ++    F  + A  Y        MM 
Sbjct: 1866 LKMYCQEELFEEAAIAVEKYEEMLKTKTLPISKLSYSASQFYLEAAAKYLSANKMKEMMA 1899

BLAST of Cucsat.G18713.T3 vs. ExPASy Swiss-Prot
Match: Q8BV79 (TPR and ankyrin repeat-containing protein 1 OS=Mus musculus OX=10090 GN=Trank1 PE=2 SV=3)

HSP 1 Score: 194.1 bits (492), Expect = 1.2e-47
Identity = 183/731 (25.03%), Postives = 324/731 (44.32%), Query Frame = 0

Query: 437  LRQLFITVTLKQCLDVKERLAYLKRISNGGNVFKETQNLCKADVLDMNDVQDLLDVPNSF 496
            L Q+F+T     C +V+     L + +   + +K           +++ +QDL D     
Sbjct: 1220 LHQIFVTKNHVLCQEVQRNFIELSKSTKATSHYKPLDP-------NVHKLQDLRD----- 1279

Query: 497  DGIPANSFPLVITFRKFLIMLDRTVGDSYFIR--------------FQKQWRLSGGKPKD 556
                  +FPL +T ++ L++LD ++   +F+R               Q+++ +   +  D
Sbjct: 1280 -----ENFPLFVTSKQLLLLLDASLPKPFFLRNEDGSLKRTIVGWSTQEEFSIPSWEEDD 1339

Query: 557  SLSRAAYNFIVSKE--------------VTVKNFASSYWSYFDSCLTYKLDAVVVFNEII 616
                A  N+   ++              VT + F +  W       +   +  +++ EI 
Sbjct: 1340 EEVEADGNYNEEEKATETQTGDSDPRVYVTFEVFTNEIWPKMIKGRS-SYNPALIWKEIK 1399

Query: 617  SQIKGGLGAKDALDGRLSKLDYTRLSKGQSTLSRKQRERIYDIFLDYEKMKNAKGEYDLA 676
            S +KG   A     GRL++  Y +L + +S   ++ R  IY +F  Y+++++ KG +D  
Sbjct: 1400 SFLKGSFEALSCPHGRLTEEAYKKLGRKRSPNFKEDRSEIYSLFCLYQQIRSQKGYFDEE 1459

Query: 677  DLVIDLHRRLKVFQYTGDHMDFVYVDEVQALTMMQITLLKYLCKNVNSGFVFSSNTTQTI 736
            D++ +L  RL   +     +  +Y DE+Q  T  ++ LL     + N+ F+ + +T Q+I
Sbjct: 1460 DVLYNLSWRLSKLRVLPWSIHELYGDEIQDFTQAELALLMKCINDPNAMFL-TGDTAQSI 1519

Query: 737  AKGIDFRFQDIRFLFYKEFISGVKTDEKGIDAGLIKIPDILHINQNCRTQPKILQLANSV 796
             KG+ FRF D+  LF+  + S    D++     + K   I  + QN R+   IL LA+ V
Sbjct: 1520 MKGVAFRFSDLLSLFH--YASRSTVDKQ---CAVRKPKRIHQLYQNYRSHSGILNLASGV 1579

Query: 797  TDLLFRFFPRCIDIVCPETSEMSSTDFETPVLLESRKGQNMMMVLFEEGRNIPADARGYG 856
             DLL  +FP   D + P  S +   D   P LL+S    ++ ++L   G         +G
Sbjct: 1580 VDLLQFYFPESFDRL-PRDSGL--FDGPKPTLLDSCSVSDLAILL--RGNKRKTQPIEFG 1639

Query: 857  AKQVILVRDECARDEISSLVGNQAIIVTIMECQCMEFQDVLLYKFFNSSPLGNQWRVIYQ 916
            A QVILV +E A+++I   +G  A+++T+ E + +EF DVLLY FF  S    +W++I  
Sbjct: 1640 AHQVILVANEKAKEKIPEELG-LALVLTVYEAKGLEFDDVLLYNFFTDSEAYKEWKIISS 1699

Query: 917  YMIEQDMLE--------IAPGGSPSFNQPVQLD------LCWELKLLHIALTRSRRRLWI 976
            +    D  E             SPS  + + ++      L  ELK L+ A+TR+R  LWI
Sbjct: 1700 FTPSSDSREEKWPLVDVPLERSSPSQARSLMVNPEMYKLLNGELKQLYTAITRARVNLWI 1759

Query: 977  YEDNQEFSNPIVDYWKKLCYVQV----KTLDYSIAQTMKVPSTKEEWSSLGLEFFSEGVY 1036
            +++N E   P   Y+ +  +VQV    +  D+  +  +K  ST  EW   G  +     +
Sbjct: 1760 FDENLEKRAPAFKYFIRRDFVQVVKTDENKDFDDSMFVKT-STPYEWIIQGDYYAKHQCW 1819

Query: 1037 GAASLCFERAEDRRRSEWARAASFCATA-----NPQISRNALREAAEIYISLDRAEIAAK 1096
              A+ C+++ +   + + A A            +P+       E A+ Y+  +  +++ K
Sbjct: 1820 KVAAKCYQKGDALEKEKLALAHYTALNMKSKKFSPKEKELQYLELAKTYLECNEPKLSLK 1879

Query: 1097 CYIELKEYKTAAYTYLTKCGEARLEDAGDCYMLAKCYKLAAVAYSMGRCFLKFFDVCTAA 1117
            C    KE++ +A                 C  L K  + AA  Y   +CF   F      
Sbjct: 1880 CLSYAKEFQLSAQL---------------CERLGK-IRDAAYFYKRSQCFQDAFRCFEQI 1903

BLAST of Cucsat.G18713.T3 vs. ExPASy Swiss-Prot
Match: Q92355 (Helicase sen1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sen1 PE=1 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 8.0e-15
Identity = 48/123 (39.02%), Postives = 75/123 (60.98%), Query Frame = 0

Query: 5    LDKFSIFTWRGAKTRLNVGVISFYAAQVSEIQSRLAHKYEKSHNFTVKVKSVDGFQGGEE 64
            L+KF    + G      +GVI+ Y +Q+ E++     KY KS   T+ +++VDGFQG E+
Sbjct: 1521 LNKFPDVNFTG-----RIGVITPYRSQLHELRRAFKVKYGKSFMSTIDIQTVDGFQGQEK 1580

Query: 65   DVIILTTVRSNRRKNIGFISSSQRINVALTRARHCLWIVGDATTLGNSNSEWEAVVSDAK 124
            D+I  + V+S  +  IGF+   +R+NVALTRAR  L I+G+  TL  ++  W ++V DA 
Sbjct: 1581 DIIFFSCVKSYSKHGIGFLRDFRRLNVALTRARSSLLIIGNMETL-KTDDLWGSLVDDAL 1637

Query: 125  DRQ 128
             R+
Sbjct: 1641 SRK 1637

BLAST of Cucsat.G18713.T3 vs. ExPASy Swiss-Prot
Match: Q00416 (Helicase SEN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SEN1 PE=1 SV=2)

HSP 1 Score: 81.3 bits (199), Expect = 1.2e-13
Identity = 38/108 (35.19%), Postives = 69/108 (63.89%), Query Frame = 0

Query: 22   VGVISFYAAQVSEIQSRLAHKYEKSHNFTVKVKSVDGFQGGEEDVIILTTVRSNRRK-NI 81
            +G+IS Y  Q+ +++   A  +    N ++   ++DGFQG E+++I+++ VR++  K ++
Sbjct: 1746 IGIISPYREQMQKMRKEFARYFGGMINKSIDFNTIDGFQGQEKEIILISCVRADDTKSSV 1805

Query: 82   GFISSSQRINVALTRARHCLWIVGDATTLGNSNSEWEAVVSDAKDRQC 129
            GF+   +R+NVALTRA+  +W++G   +L  S   W  ++ DAKDR C
Sbjct: 1806 GFLKDFRRMNVALTRAKTSIWVLGHQRSLAKSKL-WRDLIEDAKDRSC 1852

BLAST of Cucsat.G18713.T3 vs. ExPASy Swiss-Prot
Match: O74465 (Helicase required for RNAi-mediated heterochromatin assembly 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=hrr1 PE=1 SV=2)

HSP 1 Score: 80.9 bits (198), Expect = 1.5e-13
Identity = 42/112 (37.50%), Postives = 68/112 (60.71%), Query Frame = 0

Query: 5   LDKFSIFTWRGAKTRLNVGVISFYAAQVSEIQSRLAHKYEKSHNFTVKVKSVDGFQGGEE 64
           L +F+++          +  ++FYAAQ   I+  L+    +  +F +KV +VDG+QG E 
Sbjct: 842 LVQFAVYLINNGVEPQKITCLTFYAAQKDLIERLLSESLNREKHF-IKVATVDGYQGEEN 901

Query: 65  DVIILTTVRSNRRKNIGFISSSQRINVALTRARHCLWIVGDATTLGNSNSEW 117
           DV++L+ VR+N R  +GF+SS  R+ V+L+RAR  L+I G+A  +  SN  W
Sbjct: 902 DVVLLSLVRNNDRTEVGFLSSPHRVCVSLSRARRGLFIFGNAQLVAESNPLW 952

BLAST of Cucsat.G18713.T3 vs. NCBI nr
Match: XP_031741284.1 (uncharacterized protein LOC101212224 [Cucumis sativus] >XP_031741290.1 uncharacterized protein LOC116403818 [Cucumis sativus])

HSP 1 Score: 3205 bits (8309), Expect = 0.0
Identity = 1635/1647 (99.27%), Postives = 1639/1647 (99.51%), Query Frame = 0

Query: 4    FLDKFSIFTWRGAKTRLNVGVISFYAAQVSEIQSRLAHKYEKSHNFTVKVKSVDGFQGGE 63
            F+ K     WRGAKTRLNVGVISFYAAQVSEIQSRLAHKYEKSHNFTVKVKSVDGFQGGE
Sbjct: 743  FIRKNVYAAWRGAKTRLNVGVISFYAAQVSEIQSRLAHKYEKSHNFTVKVKSVDGFQGGE 802

Query: 64   EDVIILTTVRSNRRKNIGFISSSQRINVALTRARHCLWIVGDATTLGNSNSEWEAVVSDA 123
            EDVIILTTVRSNRRKNIGFISSSQRINVALTRARHCLWIVGDATTLGNSNSEWEAVVSDA
Sbjct: 803  EDVIILTTVRSNRRKNIGFISSSQRINVALTRARHCLWIVGDATTLGNSNSEWEAVVSDA 862

Query: 124  KDRQCYFNAAEDKDFADAIIEVKKVLLELDDLLNKDSVLFTMAQWKVLLSDSFRASFQNV 183
            KDRQCYFNAAEDKDFADAIIEVKKVLLELDDLLNKDSVLFTMAQWKVLLSDSFRASFQNV
Sbjct: 863  KDRQCYFNAAEDKDFADAIIEVKKVLLELDDLLNKDSVLFTMAQWKVLLSDSFRASFQNV 922

Query: 184  VSINQKKLILVLLLRLSCGWRPGTDYVPNLKCSNIIKCFKAEGLFIIYSLYIEKDLKYKQ 243
            VSINQKKLI+VLLLRLSCGWRPGTDYVPNLKCSNIIKCFKAEGLFIIYSLYIEKDLKYKQ
Sbjct: 923  VSINQKKLIIVLLLRLSCGWRPGTDYVPNLKCSNIIKCFKAEGLFIIYSLYIEKDLKYKQ 982

Query: 244  ILKIWDIKPLTDVKVLVECLSDIHELYTDDFLNLCKAKSHKGDLELPITWSASPDIVVYK 303
            ILKIWDIKPLTDVKVLVECLSDIHELYTDDFLNLCKAKSHKGDLELPITWSASPDIVVYK
Sbjct: 983  ILKIWDIKPLTDVKVLVECLSDIHELYTDDFLNLCKAKSHKGDLELPITWSASPDIVVYK 1042

Query: 304  DYMKAELNAILSLQGDSDDTQDITLKKKLLQMRFQSLSYQKAKHLLSGRDSKELDLPCQV 363
            DYMKAELNAILSLQGDSDDTQDITLKKKLLQMRFQSLSYQKAKHLLSGRDSKELDLPCQV
Sbjct: 1043 DYMKAELNAILSLQGDSDDTQDITLKKKLLQMRFQSLSYQKAKHLLSGRDSKELDLPCQV 1102

Query: 364  EDVELEIILVPTNAFIMGRPGSGKTAAMTVKLFMREQQQYIHPTGCSLVTRENAEVCYRN 423
            EDVELEIILVPTNAFIMGRPGSGKTAAMTVKLFMREQQQYIHPTGCSLVTRENAEVCYRN
Sbjct: 1103 EDVELEIILVPTNAFIMGRPGSGKTAAMTVKLFMREQQQYIHPTGCSLVTRENAEVCYRN 1162

Query: 424  EGGEECKKTERTVLRQLFITVTLKQCLDVKERLAYLKRISNGGNVFKETQNLCKADVLDM 483
            EGGEECKKTERTVLRQLFITVTLKQCL VKERLAYLKRISNGGNVFKETQNLCKADVLDM
Sbjct: 1163 EGGEECKKTERTVLRQLFITVTLKQCLYVKERLAYLKRISNGGNVFKETQNLCKADVLDM 1222

Query: 484  NDVQDLLDVPNSFDGIPANSFPLVITFRKFLIMLDRTVGDSYFIRFQKQWRLSGGKPKDS 543
            NDVQDLLDVPNSFDGIPANSFPLVITFRKFLIMLDRTVGDSYFIRFQKQWRLSGGKPKDS
Sbjct: 1223 NDVQDLLDVPNSFDGIPANSFPLVITFRKFLIMLDRTVGDSYFIRFQKQWRLSGGKPKDS 1282

Query: 544  LSRAAYNFIVSKEVTVKNFASSYWSYFDSCLTYKLDAVVVFNEIISQIKGGLGAKDALDG 603
            LSRAAYNFIVSKEVTVKNFASSYWSYFDSCLTYKLDAVVVFNEIISQIKGGLGAKDALDG
Sbjct: 1283 LSRAAYNFIVSKEVTVKNFASSYWSYFDSCLTYKLDAVVVFNEIISQIKGGLGAKDALDG 1342

Query: 604  RLSKLDYTRLSKGQSTLSRKQRERIYDIFLDYEKMKNAKGEYDLADLVIDLHRRLKVFQY 663
            +LSKLDYTRLSKGQSTLSRKQRERIYDIFLDYEKMKNAKGEYDLADLVIDLHRRLKVF+Y
Sbjct: 1343 KLSKLDYTRLSKGQSTLSRKQRERIYDIFLDYEKMKNAKGEYDLADLVIDLHRRLKVFRY 1402

Query: 664  TGDHMDFVYVDEVQALTMMQITLLKYLCKNVNSGFVFSSNTTQTIAKGIDFRFQDIRFLF 723
            TGDHMDFVYVDEVQALTMMQITLLKYLCKNVNSGFVFSSNTTQTIAKGIDFRFQDIRFLF
Sbjct: 1403 TGDHMDFVYVDEVQALTMMQITLLKYLCKNVNSGFVFSSNTTQTIAKGIDFRFQDIRFLF 1462

Query: 724  YKEFISGVKTDEKGIDAGLIKIPDILHINQNCRTQPKILQLANSVTDLLFRFFPRCIDIV 783
            YKEFISGVKTDEKGIDAGLIKIPDILHINQNCRTQPKILQLANSVTDLLFRFFPRCIDIV
Sbjct: 1463 YKEFISGVKTDEKGIDAGLIKIPDILHINQNCRTQPKILQLANSVTDLLFRFFPRCIDIV 1522

Query: 784  CPETSEMSSTDFETPVLLESRKGQNMMMVLFEEGRNIPADARGYGAKQVILVRDECARDE 843
            CPETSEMSSTDFETPVLLESRKGQNMMMVLFEEGRNIPADARGYGAKQVILVRDECARDE
Sbjct: 1523 CPETSEMSSTDFETPVLLESRKGQNMMMVLFEEGRNIPADARGYGAKQVILVRDECARDE 1582

Query: 844  ISSLVGNQAIIVTIMECQCMEFQDVLLYKFFNSSPLGNQWRVIYQYMIEQDMLEIAPGGS 903
            ISSLVGNQAIIVTIMECQCMEFQDVLLYKFFNSSPLGNQWRVIYQYMIEQDMLEIAPGGS
Sbjct: 1583 ISSLVGNQAIIVTIMECQCMEFQDVLLYKFFNSSPLGNQWRVIYQYMIEQDMLEIAPGGS 1642

Query: 904  PSFNQPVQLDLCWELKLLHIALTRSRRRLWIYEDNQEFSNPIVDYWKKLCYVQVKTLDYS 963
            PSFNQPVQLDLCWELKLLHIALTRSRRRLWIYEDNQEFSNPIVDYWKKLCYVQVKTLDYS
Sbjct: 1643 PSFNQPVQLDLCWELKLLHIALTRSRRRLWIYEDNQEFSNPIVDYWKKLCYVQVKTLDYS 1702

Query: 964  IAQTMKVPSTKEEWSSLGLEFFSEGVYGAASLCFERAEDRRRSEWARAASFCATANPQIS 1023
            I QTMKVPSTKEEWSSLGLEFFSEGVYGAASLCFERAEDRRRSEWARAASFCATANPQIS
Sbjct: 1703 IVQTMKVPSTKEEWSSLGLEFFSEGVYGAASLCFERAEDRRRSEWARAASFCATANPQIS 1762

Query: 1024 RNALREAAEIYISLDRAEIAAKCYIELKEYKTAAYTYLTKCGEARLEDAGDCYMLAKCYK 1083
            RNALREAAEIYISLDRAEIAAKCYIELKEYKTAAYTYLTKCGEARLEDAGDCYMLAKCYK
Sbjct: 1763 RNALREAAEIYISLDRAEIAAKCYIELKEYKTAAYTYLTKCGEARLEDAGDCYMLAKCYK 1822

Query: 1084 LAAVAYSMGRCFLKFFDVCTAANLFDTGLQGICSWRKYDNVDLIKKCKHIKEAWHLFLWK 1143
            LAAVAYSMGRCFLKFFDVCTAANLFDTGLQGICSWRKYDNVDLIKKCKHIKEAWHLFLWK
Sbjct: 1823 LAAVAYSMGRCFLKFFDVCTAANLFDTGLQGICSWRKYDNVDLIKKCKHIKEAWHLFLWK 1882

Query: 1144 GALHYHQLQNFGSMMRFVESFDSIDEKYLFLGTLGLSENKMLQEEELTISENEGFHSPGL 1203
            GALHYHQLQNFGSMMRFVESFDSIDEKYLFLGTLGLSENKMLQEEELTISENEGFHSPGL
Sbjct: 1883 GALHYHQLQNFGSMMRFVESFDSIDEKYLFLGTLGLSENKMLQEEELTISENEGFHSPGL 1942

Query: 1204 HLQPKLVSVSVHKETSQNDTKTKGKMKVANNISTAKGSSRGSKFQPKLKSVWKETTFQND 1263
            HLQPKLVSVSVHKETSQNDTKTKGKMKVANNISTAKGSSRGSKFQPKLKSVWKETTFQND
Sbjct: 1943 HLQPKLVSVSVHKETSQNDTKTKGKMKVANNISTAKGSSRGSKFQPKLKSVWKETTFQND 2002

Query: 1264 TKSKERMKVADDMFSLGLQFQSKLEFKTVAQIDTTIRGKMKVAENMSTTKGSSQGLKFQS 1323
            TKSKERMKVADDMFSLGLQFQSKLEFKTVAQIDTTIRGKMKVAENMSTTKGSSQGLKFQS
Sbjct: 2003 TKSKERMKVADDMFSLGLQFQSKLEFKTVAQIDTTIRGKMKVAENMSTTKGSSQGLKFQS 2062

Query: 1324 KIKSVWKETTSQYNTMTKEVELADNLSTAEEPLQGLQFQCKLEFETISQNDTTTRDSMEV 1383
            KIKSVWKETTSQYNTMTKEVELADNLSTAEEPLQGLQFQCKLEFETISQNDTTTRDSMEV
Sbjct: 2063 KIKSVWKETTSQYNTMTKEVELADNLSTAEEPLQGLQFQCKLEFETISQNDTTTRDSMEV 2122

Query: 1384 SEDMSIVNGSSKELKFQPKLKSIWKETQSRNGTKTTDKMKLANSISMSIANESSQGLQFK 1443
            SEDMSIVNGSSKELKFQPKLKSIWKETQSRNGTKTTDKMKLANSISMSIANESSQGLQFK
Sbjct: 2123 SEDMSIVNGSSKELKFQPKLKSIWKETQSRNGTKTTDKMKLANSISMSIANESSQGLQFK 2182

Query: 1444 SKLKTKTVSKNDTEKKDKIQVAEIMSTSEWSSHGLQFQSNQESLCMEKTSQNDSKIEDNL 1503
            SKLKTKTVSKNDTEKKDKIQVAEIMSTSEWSSHGLQFQSNQESLCMEKTSQNDSKIEDNL
Sbjct: 2183 SKLKTKTVSKNDTEKKDKIQVAEIMSTSEWSSHGLQFQSNQESLCMEKTSQNDSKIEDNL 2242

Query: 1504 TVAPFISSPKDTSYKLQFKPKSVYAKEVIAAQNDLKMEKDEVNIVNKAEASQRLQQQCNQ 1563
            TVAPFISSPKDTSYKLQFKPKSVYAKEVIAAQNDLKMEKDEVNIVNKAEASQRLQQQCNQ
Sbjct: 2243 TVAPFISSPKDTSYKLQFKPKSVYAKEVIAAQNDLKMEKDEVNIVNKAEASQRLQQQCNQ 2302

Query: 1564 KVRNAHKETTSSIDSKAKKDKMKNSVNLSEFGDSSQQLQQLQIEQKKLKNKNVDGEKGKQ 1623
            KVRNAHKETTSSIDSKAKKDKMKNSVNLSEFGDSSQQLQQLQIEQKKLKNKNVDGEKGKQ
Sbjct: 2303 KVRNAHKETTSSIDSKAKKDKMKNSVNLSEFGDSSQQLQQLQIEQKKLKNKNVDGEKGKQ 2362

Query: 1624 KVTDHKFIAKQYWRKVTENGMKFNFQK 1650
            KVTDHKFIAKQYWRKVTENGMKFNFQK
Sbjct: 2363 KVTDHKFIAKQYWRKVTENGMKFNFQK 2389

BLAST of Cucsat.G18713.T3 vs. NCBI nr
Match: KAE8648072.1 (hypothetical protein Csa_018863 [Cucumis sativus])

HSP 1 Score: 3187 bits (8263), Expect = 0.0
Identity = 1625/1630 (99.69%), Postives = 1628/1630 (99.88%), Query Frame = 0

Query: 13   WRGAKTRLNVGVISFYAAQVSEIQSRLAHKYEKSHNFTVKVKSVDGFQGGEEDVIILTTV 72
            WRGAKTRLNVGVISFYAAQVSEIQSRLAHKYEKSHNFTVKVKSVDGFQGGEEDVIILTTV
Sbjct: 706  WRGAKTRLNVGVISFYAAQVSEIQSRLAHKYEKSHNFTVKVKSVDGFQGGEEDVIILTTV 765

Query: 73   RSNRRKNIGFISSSQRINVALTRARHCLWIVGDATTLGNSNSEWEAVVSDAKDRQCYFNA 132
            RSNRRKNIGFISSSQRINVALTRARHCLWIVGDATTLGNSNSEWEAVVSDAKDRQCYFNA
Sbjct: 766  RSNRRKNIGFISSSQRINVALTRARHCLWIVGDATTLGNSNSEWEAVVSDAKDRQCYFNA 825

Query: 133  AEDKDFADAIIEVKKVLLELDDLLNKDSVLFTMAQWKVLLSDSFRASFQNVVSINQKKLI 192
            AEDKDFADAIIEVKKVLLELDDLLNKDSVLFTMAQWKVLLSDSFRASFQNVVSINQKKLI
Sbjct: 826  AEDKDFADAIIEVKKVLLELDDLLNKDSVLFTMAQWKVLLSDSFRASFQNVVSINQKKLI 885

Query: 193  LVLLLRLSCGWRPGTDYVPNLKCSNIIKCFKAEGLFIIYSLYIEKDLKYKQILKIWDIKP 252
            +VLLLRLSCGWRPGTDYVPNLKCSNIIKCFKAEGLFIIYSLYIEKDLKYKQILKIWDIKP
Sbjct: 886  IVLLLRLSCGWRPGTDYVPNLKCSNIIKCFKAEGLFIIYSLYIEKDLKYKQILKIWDIKP 945

Query: 253  LTDVKVLVECLSDIHELYTDDFLNLCKAKSHKGDLELPITWSASPDIVVYKDYMKAELNA 312
            LTDVKVLVECLSDIHELYTDDFLNLCKAKSHKGDLELPITWSASPDIVVYKDYMKAELNA
Sbjct: 946  LTDVKVLVECLSDIHELYTDDFLNLCKAKSHKGDLELPITWSASPDIVVYKDYMKAELNA 1005

Query: 313  ILSLQGDSDDTQDITLKKKLLQMRFQSLSYQKAKHLLSGRDSKELDLPCQVEDVELEIIL 372
            ILSLQGDSDDTQDITLKKKLLQMRFQSLSYQKAKHLLSGRDSKELDLPCQVEDVELEIIL
Sbjct: 1006 ILSLQGDSDDTQDITLKKKLLQMRFQSLSYQKAKHLLSGRDSKELDLPCQVEDVELEIIL 1065

Query: 373  VPTNAFIMGRPGSGKTAAMTVKLFMREQQQYIHPTGCSLVTRENAEVCYRNEGGEECKKT 432
            VPTNAFIMGRPGSGKTAAMTVKLFMREQQQYIHPTGCSLVTRENAEVCYRNEGGEECKKT
Sbjct: 1066 VPTNAFIMGRPGSGKTAAMTVKLFMREQQQYIHPTGCSLVTRENAEVCYRNEGGEECKKT 1125

Query: 433  ERTVLRQLFITVTLKQCLDVKERLAYLKRISNGGNVFKETQNLCKADVLDMNDVQDLLDV 492
            ERTVLRQLFITVTLKQCL VKERLAYLKRISNGGNVFKETQNLCKADVLDMNDVQDLLDV
Sbjct: 1126 ERTVLRQLFITVTLKQCLYVKERLAYLKRISNGGNVFKETQNLCKADVLDMNDVQDLLDV 1185

Query: 493  PNSFDGIPANSFPLVITFRKFLIMLDRTVGDSYFIRFQKQWRLSGGKPKDSLSRAAYNFI 552
            PNSFDGIPANSFPLVITFRKFLIMLDRTVGDSYFIRFQKQWRLSGGKPKDSLSRAAYNFI
Sbjct: 1186 PNSFDGIPANSFPLVITFRKFLIMLDRTVGDSYFIRFQKQWRLSGGKPKDSLSRAAYNFI 1245

Query: 553  VSKEVTVKNFASSYWSYFDSCLTYKLDAVVVFNEIISQIKGGLGAKDALDGRLSKLDYTR 612
            VSKEVTVKNFASSYWSYFDSCLTYKLDAVVVFNEIISQIKGGLGAKDALDG+LSKLDYTR
Sbjct: 1246 VSKEVTVKNFASSYWSYFDSCLTYKLDAVVVFNEIISQIKGGLGAKDALDGKLSKLDYTR 1305

Query: 613  LSKGQSTLSRKQRERIYDIFLDYEKMKNAKGEYDLADLVIDLHRRLKVFQYTGDHMDFVY 672
            LSKGQSTLSRKQRERIYDIFLDYEKMKNAKGEYDLADLVIDLHRRLKVF+YTGDHMDFVY
Sbjct: 1306 LSKGQSTLSRKQRERIYDIFLDYEKMKNAKGEYDLADLVIDLHRRLKVFRYTGDHMDFVY 1365

Query: 673  VDEVQALTMMQITLLKYLCKNVNSGFVFSSNTTQTIAKGIDFRFQDIRFLFYKEFISGVK 732
            VDEVQALTMMQITLLKYLCKNVNSGFVFSSNTTQTIAKGIDFRFQDIRFLFYKEFISGVK
Sbjct: 1366 VDEVQALTMMQITLLKYLCKNVNSGFVFSSNTTQTIAKGIDFRFQDIRFLFYKEFISGVK 1425

Query: 733  TDEKGIDAGLIKIPDILHINQNCRTQPKILQLANSVTDLLFRFFPRCIDIVCPETSEMSS 792
            TDEKGIDAGLIKIPDILHINQNCRTQPKILQLANSVTDLLFRFFPRCIDIVCPETSEMSS
Sbjct: 1426 TDEKGIDAGLIKIPDILHINQNCRTQPKILQLANSVTDLLFRFFPRCIDIVCPETSEMSS 1485

Query: 793  TDFETPVLLESRKGQNMMMVLFEEGRNIPADARGYGAKQVILVRDECARDEISSLVGNQA 852
            TDFETPVLLESRKGQNMMMVLFEEGRNIPADARGYGAKQVILVRDECARDEISSLVGNQA
Sbjct: 1486 TDFETPVLLESRKGQNMMMVLFEEGRNIPADARGYGAKQVILVRDECARDEISSLVGNQA 1545

Query: 853  IIVTIMECQCMEFQDVLLYKFFNSSPLGNQWRVIYQYMIEQDMLEIAPGGSPSFNQPVQL 912
            IIVTIMECQCMEFQDVLLYKFFNSSPLGNQWRVIYQYMIEQDMLEIAPGGSPSFNQPVQL
Sbjct: 1546 IIVTIMECQCMEFQDVLLYKFFNSSPLGNQWRVIYQYMIEQDMLEIAPGGSPSFNQPVQL 1605

Query: 913  DLCWELKLLHIALTRSRRRLWIYEDNQEFSNPIVDYWKKLCYVQVKTLDYSIAQTMKVPS 972
            DLCWELKLLHIALTRSRRRLWIYEDNQEFSNPIVDYWKKLCYVQVKTLDYSI QTMKVPS
Sbjct: 1606 DLCWELKLLHIALTRSRRRLWIYEDNQEFSNPIVDYWKKLCYVQVKTLDYSIVQTMKVPS 1665

Query: 973  TKEEWSSLGLEFFSEGVYGAASLCFERAEDRRRSEWARAASFCATANPQISRNALREAAE 1032
            TKEEWSSLGLEFFSEGVYGAASLCFERAEDRRRSEWARAASFCATANPQISRNALREAAE
Sbjct: 1666 TKEEWSSLGLEFFSEGVYGAASLCFERAEDRRRSEWARAASFCATANPQISRNALREAAE 1725

Query: 1033 IYISLDRAEIAAKCYIELKEYKTAAYTYLTKCGEARLEDAGDCYMLAKCYKLAAVAYSMG 1092
            IYISLDRAEIAAKCYIELKEYKTAAYTYLTKCGEARLEDAGDCYMLAKCYKLAAVAYSMG
Sbjct: 1726 IYISLDRAEIAAKCYIELKEYKTAAYTYLTKCGEARLEDAGDCYMLAKCYKLAAVAYSMG 1785

Query: 1093 RCFLKFFDVCTAANLFDTGLQGICSWRKYDNVDLIKKCKHIKEAWHLFLWKGALHYHQLQ 1152
            RCFLKFFDVCTAANLFDTGLQGICSWRKYDNVDLIKKCKHIKEAWHLFLWKGALHYHQLQ
Sbjct: 1786 RCFLKFFDVCTAANLFDTGLQGICSWRKYDNVDLIKKCKHIKEAWHLFLWKGALHYHQLQ 1845

Query: 1153 NFGSMMRFVESFDSIDEKYLFLGTLGLSENKMLQEEELTISENEGFHSPGLHLQPKLVSV 1212
            NFGSMMRFVESFDSIDEKYLFLGTLGLSENKMLQEEELTISENEGFHSPGLHLQPKLVSV
Sbjct: 1846 NFGSMMRFVESFDSIDEKYLFLGTLGLSENKMLQEEELTISENEGFHSPGLHLQPKLVSV 1905

Query: 1213 SVHKETSQNDTKTKGKMKVANNISTAKGSSRGSKFQPKLKSVWKETTFQNDTKSKERMKV 1272
            SVHKETSQNDTKTKGKMKVANNISTAKGSSRGSKFQPKLKSVWKETTFQNDTKSKERMKV
Sbjct: 1906 SVHKETSQNDTKTKGKMKVANNISTAKGSSRGSKFQPKLKSVWKETTFQNDTKSKERMKV 1965

Query: 1273 ADDMFSLGLQFQSKLEFKTVAQIDTTIRGKMKVAENMSTTKGSSQGLKFQSKIKSVWKET 1332
            ADDMFSLGLQFQSKLEFKTVAQIDTTIRGKMKVAENMSTTKGSSQGLKFQSKIKSVWKET
Sbjct: 1966 ADDMFSLGLQFQSKLEFKTVAQIDTTIRGKMKVAENMSTTKGSSQGLKFQSKIKSVWKET 2025

Query: 1333 TSQYNTMTKEVELADNLSTAEEPLQGLQFQCKLEFETISQNDTTTRDSMEVSEDMSIVNG 1392
            TSQYNTMTKEVELADNLSTAEEPLQGLQFQCKLEFETISQNDTTTRDSMEVSEDMSIVNG
Sbjct: 2026 TSQYNTMTKEVELADNLSTAEEPLQGLQFQCKLEFETISQNDTTTRDSMEVSEDMSIVNG 2085

Query: 1393 SSKELKFQPKLKSIWKETQSRNGTKTTDKMKLANSISMSIANESSQGLQFKSKLKTKTVS 1452
            SSKELKFQPKLKSIWKETQSRNGTKTTDKMKLANSISMSIANESSQGLQFKSKLKTKTVS
Sbjct: 2086 SSKELKFQPKLKSIWKETQSRNGTKTTDKMKLANSISMSIANESSQGLQFKSKLKTKTVS 2145

Query: 1453 KNDTEKKDKIQVAEIMSTSEWSSHGLQFQSNQESLCMEKTSQNDSKIEDNLTVAPFISSP 1512
            KNDTEKKDKIQVAEIMSTSEWSSHGLQFQSNQESLCMEKTSQNDSKIEDNLTVAPFISSP
Sbjct: 2146 KNDTEKKDKIQVAEIMSTSEWSSHGLQFQSNQESLCMEKTSQNDSKIEDNLTVAPFISSP 2205

Query: 1513 KDTSYKLQFKPKSVYAKEVIAAQNDLKMEKDEVNIVNKAEASQRLQQQCNQKVRNAHKET 1572
            KDTSYKLQFKPKSVYAKEVIAAQNDLKMEKDEVNIVNKAEASQRLQQQCNQKVRNAHKET
Sbjct: 2206 KDTSYKLQFKPKSVYAKEVIAAQNDLKMEKDEVNIVNKAEASQRLQQQCNQKVRNAHKET 2265

Query: 1573 TSSIDSKAKKDKMKNSVNLSEFGDSSQQLQQLQIEQKKLKNKNVDGEKGKQKVTDHKFIA 1632
            TSSIDSKAKKDKMKNSVNLSEFGDSSQQLQQLQIEQKKLKNKNVDGEKGKQKVTDHKFIA
Sbjct: 2266 TSSIDSKAKKDKMKNSVNLSEFGDSSQQLQQLQIEQKKLKNKNVDGEKGKQKVTDHKFIA 2325

Query: 1633 KQYWRKVTEN 1642
            KQYWRKVTEN
Sbjct: 2326 KQYWRKVTEN 2335

BLAST of Cucsat.G18713.T3 vs. NCBI nr
Match: XP_038876924.1 (uncharacterized protein LOC120069278 [Benincasa hispida] >XP_038876925.1 uncharacterized protein LOC120069278 [Benincasa hispida])

HSP 1 Score: 1891 bits (4899), Expect = 0.0
Identity = 959/1200 (79.92%), Postives = 1045/1200 (87.08%), Query Frame = 0

Query: 13   WRGAKTRLNVGVISFYAAQVSEIQSRLAHKYEKSHNFTVKVKSVDGFQGGEEDVIILTTV 72
            WR AKTRL++GVISFYAAQVS IQ RL  KYEKS  FTVKVKSVDGFQGGEEDVIIL+TV
Sbjct: 717  WRSAKTRLSIGVISFYAAQVSAIQGRLGQKYEKSDKFTVKVKSVDGFQGGEEDVIILSTV 776

Query: 73   RSNRRKNIGFISSSQRINVALTRARHCLWIVGDATTLGNSNSEWEAVVSDAKDRQCYFNA 132
            RSNRRKNIGFIS+SQRINVALTRARHCLWIVGDATTLG+SNSEWEAVVSDAKDRQCYFNA
Sbjct: 777  RSNRRKNIGFISNSQRINVALTRARHCLWIVGDATTLGDSNSEWEAVVSDAKDRQCYFNA 836

Query: 133  AEDKDFADAIIEVKKVLLELDDLLNKDSVLFTMAQWKVLLSDSFRASFQNVVSINQKKLI 192
             EDKD ADAIIEVKKVLLELDDLLNKDS LF M QWKVLLSDSFRASFQ VVSINQKK I
Sbjct: 837  EEDKDLADAIIEVKKVLLELDDLLNKDSALFKMVQWKVLLSDSFRASFQEVVSINQKKSI 896

Query: 193  LVLLLRLSCGWRPGTDYVPNLKCSNIIKCFKAEGLFIIYSLYIEKDLKYKQILKIWDIKP 252
            +VLLLRLSCGWRP T+ V N KCS+IIKC K EGLFIIYSL IEKD KYKQ+LKIWDIKP
Sbjct: 897  IVLLLRLSCGWRPETN-VSNPKCSDIIKCVKVEGLFIIYSLDIEKDSKYKQVLKIWDIKP 956

Query: 253  LTDVKVLVECLSDIHELYTDDFLNLCKAKSHKGDLELPITWSASPDIVVYKDYMKAELNA 312
            LTDVK LV+CLS+IHELYTDDFLNLCK KS KGDLELPITWSAS DIVVYKD+MKAEL+A
Sbjct: 957  LTDVKGLVDCLSNIHELYTDDFLNLCKTKSDKGDLELPITWSASHDIVVYKDHMKAELDA 1016

Query: 313  ILSLQGDSDDTQDITLKKKLLQMRFQSLSYQKAKHLLSGRDSKELDLPCQVEDVELEIIL 372
            ILSLQ DSDDT++ITLKK LLQM+FQSLSYQKAKHLLS  DSKELDLPCQVED +LEIIL
Sbjct: 1017 ILSLQADSDDTKNITLKKNLLQMKFQSLSYQKAKHLLSSHDSKELDLPCQVEDEQLEIIL 1076

Query: 373  VPTNAFIMGRPGSGKTAAMTVKLFMREQQQYIHPTGCSLVTRENAEVCYRNEGGEECKKT 432
             PT+AF+MGRP  GKTAA+T+KLFMREQQQ IHP GCS VTR+NAEVC RNEGGEECK+ 
Sbjct: 1077 CPTSAFLMGRPSYGKTAALTIKLFMREQQQQIHPEGCSEVTRQNAEVCCRNEGGEECKRI 1136

Query: 433  ERTVLRQLFITVTLKQCLDVKERLAYLKRISNGGNVFKETQNLCKADVLDMNDVQDLLDV 492
             RTVLRQLFITVTLKQCL VKE L+YLKRISNGGN+ +E Q+  K DVLDM+D QDLLDV
Sbjct: 1137 GRTVLRQLFITVTLKQCLAVKEHLSYLKRISNGGNILEENQSFNKVDVLDMDDAQDLLDV 1196

Query: 493  PNSFDGIPANSFPLVITFRKFLIMLDRTVGDSYFIRFQKQWRLSGGKPKDSLSRAAYNFI 552
            PNSFDGIP NS+PLVITFRKFL+MLDRTVGDS+  RFQKQW+LS GK +D LS A Y FI
Sbjct: 1197 PNSFDGIPFNSYPLVITFRKFLMMLDRTVGDSFLFRFQKQWKLSCGKARDPLSTAVYKFI 1256

Query: 553  VSKEVTVKNFASSYWSYFDSCLTYKLDAVVVFNEIISQIKGGLGAKDALDGRLSKLDYTR 612
             SKEVT+K FASSYWSYF   LT KLDAVVVFNEIISQIKGG+GAK+AL GRLSK+DYT 
Sbjct: 1257 GSKEVTIKRFASSYWSYFGDHLTNKLDAVVVFNEIISQIKGGIGAKEALGGRLSKVDYTG 1316

Query: 613  LSKGQSTLSRKQRERIYDIFLDYEKMKNAKGEYDLADLVIDLHRRLKVFQYTGDHMDFVY 672
            L+KGQS LSRKQRERIYDIFLDYEKMKN K EYDLAD+VIDLH RLK FQY GD MDFVY
Sbjct: 1317 LAKGQSALSRKQRERIYDIFLDYEKMKNEKREYDLADIVIDLHHRLKGFQYMGDRMDFVY 1376

Query: 673  VDEVQALTMMQITLLKYLCKNVNSGFVFSSNTTQTIAKGIDFRFQDIRFLFYKEFISGVK 732
            VDEVQALTMM I LLKYLC NV+SGFVFSSNT QTIAKGIDFRFQDIRFLFYKEF+S VK
Sbjct: 1377 VDEVQALTMMDIALLKYLCGNVSSGFVFSSNTAQTIAKGIDFRFQDIRFLFYKEFMSRVK 1436

Query: 733  TDEKGIDAGLIKIPDILHINQNCRTQPKILQLANSVTDLLFRFFPRCIDIVCPETSEMSS 792
            TDEK  DAG +KIPDILH+NQNC TQPKILQLANSVTDLLFRFFP C+DI+CPETSEMS 
Sbjct: 1437 TDEKD-DAGFLKIPDILHMNQNCCTQPKILQLANSVTDLLFRFFPWCVDILCPETSEMSP 1496

Query: 793  TDFETPVLLESRKGQNMMMVLFEEGRNIPADARGYGAKQVILVRDECARDEISSLVGNQA 852
             +FE P+L+E+ KGQNMM VLFE   NIPAD    GAKQVILVRDE  R+EIS+LVGNQA
Sbjct: 1497 ANFEAPILIENGKGQNMMTVLFEGTGNIPADTHDVGAKQVILVRDEHGRNEISNLVGNQA 1556

Query: 853  IIVTIMECQCMEFQDVLLYKFFNSSPLGNQWRVIYQYMIEQDMLEIAPGGSPSFNQPVQL 912
            I++TIMECQ +EFQDVLLY FF SSPLG+QWRVIYQYMIEQDMLEIA   SP+FNQPV++
Sbjct: 1557 IVLTIMECQSLEFQDVLLYNFFTSSPLGHQWRVIYQYMIEQDMLEIA-YNSPNFNQPVRM 1616

Query: 913  DLCWELKLLHIALTRSRRRLWIYEDNQEFSNPIVDYWKKLCYVQVKTLDYSIAQTMKVPS 972
            DLCWELKLLHIA+TR R+RLWIYEDNQEF NP+VDYWKKLCY+Q+KTLDYSI Q MK  S
Sbjct: 1617 DLCWELKLLHIAITRCRQRLWIYEDNQEFPNPMVDYWKKLCYIQIKTLDYSIVQAMKAQS 1676

Query: 973  TKEEWSSLGLEFFSEGVYGAASLCFERAEDRRRSEWARAASFCATA------NPQISRNA 1032
            TKEEWSSLGLE FSEGVYGAASLCFERAED  R EWARAAS CATA      NPQ++ NA
Sbjct: 1677 TKEEWSSLGLELFSEGVYGAASLCFERAEDGLRREWARAASLCATAGILDGSNPQMACNA 1736

Query: 1033 LREAAEIYISLDRAEIAAKCYIELKEYKTAAYTYLTKCGEARLEDAGDCYMLAKCYKLAA 1092
            LREAAEIYIS+DRAE AAKC+IELKEYK+AA  YLTKCGEA+LEDAGDCYMLA+CY+LAA
Sbjct: 1737 LREAAEIYISMDRAEAAAKCFIELKEYKSAANMYLTKCGEAKLEDAGDCYMLAECYELAA 1796

Query: 1093 VAYSMGRCFLKFFDVCTAANLFDTGLQGICSWRKYDNVDLIKKCKHIKEAWHLFLWKGAL 1152
             AYS GRCFLKF +VCT ANLFD GLQ +CSWR  ++ D I KC+ IKE WHLFL KGAL
Sbjct: 1797 GAYSRGRCFLKFLNVCTVANLFDMGLQVMCSWRNCNDDDPIVKCEDIKEVWHLFLKKGAL 1856

Query: 1153 HYHQLQNFGSMMRFVESFDSIDEKYLFLGTLGLSENKMLQEEELTISENEGFHSPGLHLQ 1206
            HYHQLQ+F  MM+FVE+FDS+DEK  FL TLG+SE  +L E+E+  S N      G+ L+
Sbjct: 1857 HYHQLQDFRFMMKFVETFDSMDEKCSFLRTLGISEKILLLEKEVEESLNIMMKKGGISLE 1913

BLAST of Cucsat.G18713.T3 vs. NCBI nr
Match: XP_023515720.1 (uncharacterized protein LOC111779783 isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1881 bits (4872), Expect = 0.0
Identity = 953/1191 (80.02%), Postives = 1042/1191 (87.49%), Query Frame = 0

Query: 13   WRGAKTRLNVGVISFYAAQVSEIQSRLAHKYEKSHNFTVKVKSVDGFQGGEEDVIILTTV 72
            WR AKTRLNVGVISFYAAQVS IQSRL HKYEKS NFTVKVKSVD FQGGEEDVIILTTV
Sbjct: 678  WRKAKTRLNVGVISFYAAQVSAIQSRLGHKYEKSGNFTVKVKSVDDFQGGEEDVIILTTV 737

Query: 73   RSNRRKNIGFISSSQRINVALTRARHCLWIVGDATTLGNSNSEWEAVVSDAKDRQCYFNA 132
            RSNRR NIGFIS+SQRINVALTRARHCLWIVGDATTLGNSNSEWE+VVS+AKDRQCYFNA
Sbjct: 738  RSNRRSNIGFISNSQRINVALTRARHCLWIVGDATTLGNSNSEWESVVSNAKDRQCYFNA 797

Query: 133  AEDKDFADAIIEVKKVLLELDDLLNKDSVLFTMAQWKVLLSDSFRASFQNVVSINQKKLI 192
             EDKD ADAII VKKVLLELDDLLNKDSVLF + QWKVLLSDSFRASFQ VVSINQKK I
Sbjct: 798  EEDKDLADAIIGVKKVLLELDDLLNKDSVLFKLVQWKVLLSDSFRASFQKVVSINQKKSI 857

Query: 193  LVLLLRLSCGWRPGTDYVPNLKCSNIIKCFKAEGLFIIYSLYIEKDLKYKQILKIWDIKP 252
            +VLLLRL+CGWRP  + V N KCSNII   K EGLFI+YSL IEKD KYKQ+LKIWDIKP
Sbjct: 858  IVLLLRLACGWRPEANSVSNTKCSNIISV-KVEGLFIVYSLDIEKDSKYKQVLKIWDIKP 917

Query: 253  LTDVKVLVECLSDIHELYTDDFLNLCKAKSHKGDLELPITWSASPDIVVYKDYMKAELNA 312
            L DVKVLVECLS+IHELYTDDFLNLCKAKSHKGDLELPITWSAS D+V+YKD+MKAEL+A
Sbjct: 918  LADVKVLVECLSNIHELYTDDFLNLCKAKSHKGDLELPITWSASLDVVMYKDHMKAELDA 977

Query: 313  ILSLQGDSDDTQDITLKKKLLQMRFQSLSYQKAKHLLSGRDSKELDLPCQVEDVELEIIL 372
            ILSLQ DSDD ++ TLKK LLQM+FQSLSY KAKHLLS  DSKELDLPCQVED +LEIIL
Sbjct: 978  ILSLQADSDDIKNSTLKKNLLQMKFQSLSYLKAKHLLSRHDSKELDLPCQVEDEQLEIIL 1037

Query: 373  VPTNAFIMGRPGSGKTAAMTVKLFMREQQQYIHPTGCSLVTRENAEVCYRNEGGEECKKT 432
             PT+AFIMGRP SGKTAA+T+KLFMREQQQ IH  GCS VTRENAEV YRN+ GE CKK 
Sbjct: 1038 FPTSAFIMGRPDSGKTAALTIKLFMREQQQQIHSGGCSQVTRENAEVGYRNDDGEACKKI 1097

Query: 433  ERTVLRQLFITVTLKQCLDVKERLAYLKRISNGGNVFKETQNLCKADVLDMNDVQDLLDV 492
            +RTVLRQLFIT TLKQC  VKE L+YLKRIS GGN+ +E Q   K  V+DM+D QDLLDV
Sbjct: 1098 DRTVLRQLFITATLKQCQAVKEHLSYLKRISTGGNILEENQKFNKVGVMDMDDAQDLLDV 1157

Query: 493  PNSFDGIPANSFPLVITFRKFLIMLDRTVGDSYFIRFQKQWRLSGGKPKDSLSRAAYNFI 552
            PNSFDGIP +S+PLVITFRKFLIM+DRTVGDS+ +RF KQW+LS GKP+D LS AAYNFI
Sbjct: 1158 PNSFDGIPFSSYPLVITFRKFLIMVDRTVGDSFLVRFLKQWKLSCGKPRDPLSTAAYNFI 1217

Query: 553  VSKEVTVKNFASSYWSYFDSCLTYKLDAVVVFNEIISQIKGGLGAKDALDGRLSKLDYTR 612
             SKEVTVK FASSYWSYFD CLT  LDAV+VFNEIISQIKGGLGAK+  DGRLSKLDYTR
Sbjct: 1218 ESKEVTVKKFASSYWSYFDGCLTNNLDAVMVFNEIISQIKGGLGAKETPDGRLSKLDYTR 1277

Query: 613  LSKGQSTLSRKQRERIYDIFLDYEKMKNAKGEYDLADLVIDLHRRLKVFQYTGDHMDFVY 672
            L+KG+STLS KQRERIYDIFLDYE+MKN KGEYDLADLVIDLH RLK  QYTGD MD+VY
Sbjct: 1278 LAKGRSTLSWKQRERIYDIFLDYERMKNEKGEYDLADLVIDLHHRLKCSQYTGDQMDYVY 1337

Query: 673  VDEVQALTMMQITLLKYLCKNVNSGFVFSSNTTQTIAKGIDFRFQDIRFLFYKEFISGVK 732
            VDEVQALTMM+I LLKYLC NV+SGFVFSSNT QTIAKGIDFRF DIRFLFYKEFIS VK
Sbjct: 1338 VDEVQALTMMEIALLKYLCGNVSSGFVFSSNTAQTIAKGIDFRFHDIRFLFYKEFISRVK 1397

Query: 733  TDEKGIDAGLIKIPDILHINQNCRTQPKILQLANSVTDLLFRFFPRCIDIVCPETSEMSS 792
            TDEK I AGL+KIPDILH+NQNC TQPKILQLA+SVTDLLFRFFP CIDI+CPETSEMSS
Sbjct: 1398 TDEKDIGAGLLKIPDILHMNQNCHTQPKILQLASSVTDLLFRFFPHCIDILCPETSEMSS 1457

Query: 793  TDFETPVLLESRKGQNMMMVLFEEGRNIPADARGYGAKQVILVRDECARDEISSLVGNQA 852
             +FETPVLLE+ KGQNMM +LF    NIPAD R +GAKQVILVRDE ARD IS+LV NQA
Sbjct: 1458 GNFETPVLLENGKGQNMMTLLFGGTGNIPADTREFGAKQVILVRDEHARDGISNLVRNQA 1517

Query: 853  IIVTIMECQCMEFQDVLLYKFFNSSPLGNQWRVIYQYMIEQDMLEIAPGGSPSFNQPVQL 912
            I++TIMECQ +EFQDVLLY FFNSSPLG+QW VIYQYMIEQDMLEIAP  SP+FNQPV +
Sbjct: 1518 IVLTIMECQSLEFQDVLLYNFFNSSPLGHQWSVIYQYMIEQDMLEIAPN-SPNFNQPVHM 1577

Query: 913  DLCWELKLLHIALTRSRRRLWIYEDNQEFSNPIVDYWKKLCYVQVKTLDYSIAQTMKVPS 972
            DLCWELKLLHIA+TRSR+RLWIYED+QEF NPIVDYWKKLCY+QVKTLDYSI QTMK PS
Sbjct: 1578 DLCWELKLLHIAITRSRQRLWIYEDSQEFPNPIVDYWKKLCYIQVKTLDYSIIQTMKAPS 1637

Query: 973  TKEEWSSLGLEFFSEGVYGAASLCFERAEDRRRSEWARAASFCATA------NPQISRNA 1032
            TKEEWSSLGLEFF EGVY AASLCFERA+DR R  WARAAS  ATA      NPQ++RNA
Sbjct: 1638 TKEEWSSLGLEFFCEGVYVAASLCFERADDRLRRAWARAASLRATACILDGSNPQMARNA 1697

Query: 1033 LREAAEIYISLDRAEIAAKCYIELKEYKTAAYTYLTKCGEARLEDAGDCYMLAKCYKLAA 1092
            L+EAAEIYIS+DRAE+AAKC+IELKEY+TAAY Y  KCGEA+LEDAGDCYMLA+CY+LAA
Sbjct: 1698 LQEAAEIYISMDRAEVAAKCFIELKEYQTAAYIYSKKCGEAKLEDAGDCYMLAECYELAA 1757

Query: 1093 VAYSMGRCFLKFFDVCTAANLFDTGLQGICSWRKY--DNVDLIKKCKHIKEAWHLFLWKG 1152
             AYS GR FLKF +VCT ANLFD GLQ +CSWRK+  D+ DLI+KC   KE WH+FL KG
Sbjct: 1758 EAYSRGRFFLKFLNVCTVANLFDMGLQVVCSWRKHCDDDDDLIEKCLDFKEIWHVFLQKG 1817

Query: 1153 ALHYHQLQNFGSMMRFVESFDSIDEKYLFLGTLGLSENKMLQEEELTISEN 1195
            ALHYHQLQ+F S+++FV+ FDS+DEK  FL TLGLSE  +L E+++    N
Sbjct: 1818 ALHYHQLQDFRSILKFVDIFDSMDEKCSFLRTLGLSEKILLLEKDVEEDTN 1866

BLAST of Cucsat.G18713.T3 vs. NCBI nr
Match: XP_023515693.1 (uncharacterized protein LOC111779783 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023515702.1 uncharacterized protein LOC111779783 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023515711.1 uncharacterized protein LOC111779783 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1881 bits (4872), Expect = 0.0
Identity = 953/1191 (80.02%), Postives = 1042/1191 (87.49%), Query Frame = 0

Query: 13   WRGAKTRLNVGVISFYAAQVSEIQSRLAHKYEKSHNFTVKVKSVDGFQGGEEDVIILTTV 72
            WR AKTRLNVGVISFYAAQVS IQSRL HKYEKS NFTVKVKSVD FQGGEEDVIILTTV
Sbjct: 717  WRKAKTRLNVGVISFYAAQVSAIQSRLGHKYEKSGNFTVKVKSVDDFQGGEEDVIILTTV 776

Query: 73   RSNRRKNIGFISSSQRINVALTRARHCLWIVGDATTLGNSNSEWEAVVSDAKDRQCYFNA 132
            RSNRR NIGFIS+SQRINVALTRARHCLWIVGDATTLGNSNSEWE+VVS+AKDRQCYFNA
Sbjct: 777  RSNRRSNIGFISNSQRINVALTRARHCLWIVGDATTLGNSNSEWESVVSNAKDRQCYFNA 836

Query: 133  AEDKDFADAIIEVKKVLLELDDLLNKDSVLFTMAQWKVLLSDSFRASFQNVVSINQKKLI 192
             EDKD ADAII VKKVLLELDDLLNKDSVLF + QWKVLLSDSFRASFQ VVSINQKK I
Sbjct: 837  EEDKDLADAIIGVKKVLLELDDLLNKDSVLFKLVQWKVLLSDSFRASFQKVVSINQKKSI 896

Query: 193  LVLLLRLSCGWRPGTDYVPNLKCSNIIKCFKAEGLFIIYSLYIEKDLKYKQILKIWDIKP 252
            +VLLLRL+CGWRP  + V N KCSNII   K EGLFI+YSL IEKD KYKQ+LKIWDIKP
Sbjct: 897  IVLLLRLACGWRPEANSVSNTKCSNIISV-KVEGLFIVYSLDIEKDSKYKQVLKIWDIKP 956

Query: 253  LTDVKVLVECLSDIHELYTDDFLNLCKAKSHKGDLELPITWSASPDIVVYKDYMKAELNA 312
            L DVKVLVECLS+IHELYTDDFLNLCKAKSHKGDLELPITWSAS D+V+YKD+MKAEL+A
Sbjct: 957  LADVKVLVECLSNIHELYTDDFLNLCKAKSHKGDLELPITWSASLDVVMYKDHMKAELDA 1016

Query: 313  ILSLQGDSDDTQDITLKKKLLQMRFQSLSYQKAKHLLSGRDSKELDLPCQVEDVELEIIL 372
            ILSLQ DSDD ++ TLKK LLQM+FQSLSY KAKHLLS  DSKELDLPCQVED +LEIIL
Sbjct: 1017 ILSLQADSDDIKNSTLKKNLLQMKFQSLSYLKAKHLLSRHDSKELDLPCQVEDEQLEIIL 1076

Query: 373  VPTNAFIMGRPGSGKTAAMTVKLFMREQQQYIHPTGCSLVTRENAEVCYRNEGGEECKKT 432
             PT+AFIMGRP SGKTAA+T+KLFMREQQQ IH  GCS VTRENAEV YRN+ GE CKK 
Sbjct: 1077 FPTSAFIMGRPDSGKTAALTIKLFMREQQQQIHSGGCSQVTRENAEVGYRNDDGEACKKI 1136

Query: 433  ERTVLRQLFITVTLKQCLDVKERLAYLKRISNGGNVFKETQNLCKADVLDMNDVQDLLDV 492
            +RTVLRQLFIT TLKQC  VKE L+YLKRIS GGN+ +E Q   K  V+DM+D QDLLDV
Sbjct: 1137 DRTVLRQLFITATLKQCQAVKEHLSYLKRISTGGNILEENQKFNKVGVMDMDDAQDLLDV 1196

Query: 493  PNSFDGIPANSFPLVITFRKFLIMLDRTVGDSYFIRFQKQWRLSGGKPKDSLSRAAYNFI 552
            PNSFDGIP +S+PLVITFRKFLIM+DRTVGDS+ +RF KQW+LS GKP+D LS AAYNFI
Sbjct: 1197 PNSFDGIPFSSYPLVITFRKFLIMVDRTVGDSFLVRFLKQWKLSCGKPRDPLSTAAYNFI 1256

Query: 553  VSKEVTVKNFASSYWSYFDSCLTYKLDAVVVFNEIISQIKGGLGAKDALDGRLSKLDYTR 612
             SKEVTVK FASSYWSYFD CLT  LDAV+VFNEIISQIKGGLGAK+  DGRLSKLDYTR
Sbjct: 1257 ESKEVTVKKFASSYWSYFDGCLTNNLDAVMVFNEIISQIKGGLGAKETPDGRLSKLDYTR 1316

Query: 613  LSKGQSTLSRKQRERIYDIFLDYEKMKNAKGEYDLADLVIDLHRRLKVFQYTGDHMDFVY 672
            L+KG+STLS KQRERIYDIFLDYE+MKN KGEYDLADLVIDLH RLK  QYTGD MD+VY
Sbjct: 1317 LAKGRSTLSWKQRERIYDIFLDYERMKNEKGEYDLADLVIDLHHRLKCSQYTGDQMDYVY 1376

Query: 673  VDEVQALTMMQITLLKYLCKNVNSGFVFSSNTTQTIAKGIDFRFQDIRFLFYKEFISGVK 732
            VDEVQALTMM+I LLKYLC NV+SGFVFSSNT QTIAKGIDFRF DIRFLFYKEFIS VK
Sbjct: 1377 VDEVQALTMMEIALLKYLCGNVSSGFVFSSNTAQTIAKGIDFRFHDIRFLFYKEFISRVK 1436

Query: 733  TDEKGIDAGLIKIPDILHINQNCRTQPKILQLANSVTDLLFRFFPRCIDIVCPETSEMSS 792
            TDEK I AGL+KIPDILH+NQNC TQPKILQLA+SVTDLLFRFFP CIDI+CPETSEMSS
Sbjct: 1437 TDEKDIGAGLLKIPDILHMNQNCHTQPKILQLASSVTDLLFRFFPHCIDILCPETSEMSS 1496

Query: 793  TDFETPVLLESRKGQNMMMVLFEEGRNIPADARGYGAKQVILVRDECARDEISSLVGNQA 852
             +FETPVLLE+ KGQNMM +LF    NIPAD R +GAKQVILVRDE ARD IS+LV NQA
Sbjct: 1497 GNFETPVLLENGKGQNMMTLLFGGTGNIPADTREFGAKQVILVRDEHARDGISNLVRNQA 1556

Query: 853  IIVTIMECQCMEFQDVLLYKFFNSSPLGNQWRVIYQYMIEQDMLEIAPGGSPSFNQPVQL 912
            I++TIMECQ +EFQDVLLY FFNSSPLG+QW VIYQYMIEQDMLEIAP  SP+FNQPV +
Sbjct: 1557 IVLTIMECQSLEFQDVLLYNFFNSSPLGHQWSVIYQYMIEQDMLEIAPN-SPNFNQPVHM 1616

Query: 913  DLCWELKLLHIALTRSRRRLWIYEDNQEFSNPIVDYWKKLCYVQVKTLDYSIAQTMKVPS 972
            DLCWELKLLHIA+TRSR+RLWIYED+QEF NPIVDYWKKLCY+QVKTLDYSI QTMK PS
Sbjct: 1617 DLCWELKLLHIAITRSRQRLWIYEDSQEFPNPIVDYWKKLCYIQVKTLDYSIIQTMKAPS 1676

Query: 973  TKEEWSSLGLEFFSEGVYGAASLCFERAEDRRRSEWARAASFCATA------NPQISRNA 1032
            TKEEWSSLGLEFF EGVY AASLCFERA+DR R  WARAAS  ATA      NPQ++RNA
Sbjct: 1677 TKEEWSSLGLEFFCEGVYVAASLCFERADDRLRRAWARAASLRATACILDGSNPQMARNA 1736

Query: 1033 LREAAEIYISLDRAEIAAKCYIELKEYKTAAYTYLTKCGEARLEDAGDCYMLAKCYKLAA 1092
            L+EAAEIYIS+DRAE+AAKC+IELKEY+TAAY Y  KCGEA+LEDAGDCYMLA+CY+LAA
Sbjct: 1737 LQEAAEIYISMDRAEVAAKCFIELKEYQTAAYIYSKKCGEAKLEDAGDCYMLAECYELAA 1796

Query: 1093 VAYSMGRCFLKFFDVCTAANLFDTGLQGICSWRKY--DNVDLIKKCKHIKEAWHLFLWKG 1152
             AYS GR FLKF +VCT ANLFD GLQ +CSWRK+  D+ DLI+KC   KE WH+FL KG
Sbjct: 1797 EAYSRGRFFLKFLNVCTVANLFDMGLQVVCSWRKHCDDDDDLIEKCLDFKEIWHVFLQKG 1856

Query: 1153 ALHYHQLQNFGSMMRFVESFDSIDEKYLFLGTLGLSENKMLQEEELTISEN 1195
            ALHYHQLQ+F S+++FV+ FDS+DEK  FL TLGLSE  +L E+++    N
Sbjct: 1857 ALHYHQLQDFRSILKFVDIFDSMDEKCSFLRTLGLSEKILLLEKDVEEDTN 1905

BLAST of Cucsat.G18713.T3 vs. ExPASy TrEMBL
Match: A0A0A0KQV9 (AAA_12 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G266850 PE=4 SV=1)

HSP 1 Score: 3205 bits (8309), Expect = 0.0
Identity = 1635/1647 (99.27%), Postives = 1639/1647 (99.51%), Query Frame = 0

Query: 4    FLDKFSIFTWRGAKTRLNVGVISFYAAQVSEIQSRLAHKYEKSHNFTVKVKSVDGFQGGE 63
            F+ K     WRGAKTRLNVGVISFYAAQVSEIQSRLAHKYEKSHNFTVKVKSVDGFQGGE
Sbjct: 6    FIRKNVYAAWRGAKTRLNVGVISFYAAQVSEIQSRLAHKYEKSHNFTVKVKSVDGFQGGE 65

Query: 64   EDVIILTTVRSNRRKNIGFISSSQRINVALTRARHCLWIVGDATTLGNSNSEWEAVVSDA 123
            EDVIILTTVRSNRRKNIGFISSSQRINVALTRARHCLWIVGDATTLGNSNSEWEAVVSDA
Sbjct: 66   EDVIILTTVRSNRRKNIGFISSSQRINVALTRARHCLWIVGDATTLGNSNSEWEAVVSDA 125

Query: 124  KDRQCYFNAAEDKDFADAIIEVKKVLLELDDLLNKDSVLFTMAQWKVLLSDSFRASFQNV 183
            KDRQCYFNAAEDKDFADAIIEVKKVLLELDDLLNKDSVLFTMAQWKVLLSDSFRASFQNV
Sbjct: 126  KDRQCYFNAAEDKDFADAIIEVKKVLLELDDLLNKDSVLFTMAQWKVLLSDSFRASFQNV 185

Query: 184  VSINQKKLILVLLLRLSCGWRPGTDYVPNLKCSNIIKCFKAEGLFIIYSLYIEKDLKYKQ 243
            VSINQKKLI+VLLLRLSCGWRPGTDYVPNLKCSNIIKCFKAEGLFIIYSLYIEKDLKYKQ
Sbjct: 186  VSINQKKLIIVLLLRLSCGWRPGTDYVPNLKCSNIIKCFKAEGLFIIYSLYIEKDLKYKQ 245

Query: 244  ILKIWDIKPLTDVKVLVECLSDIHELYTDDFLNLCKAKSHKGDLELPITWSASPDIVVYK 303
            ILKIWDIKPLTDVKVLVECLSDIHELYTDDFLNLCKAKSHKGDLELPITWSASPDIVVYK
Sbjct: 246  ILKIWDIKPLTDVKVLVECLSDIHELYTDDFLNLCKAKSHKGDLELPITWSASPDIVVYK 305

Query: 304  DYMKAELNAILSLQGDSDDTQDITLKKKLLQMRFQSLSYQKAKHLLSGRDSKELDLPCQV 363
            DYMKAELNAILSLQGDSDDTQDITLKKKLLQMRFQSLSYQKAKHLLSGRDSKELDLPCQV
Sbjct: 306  DYMKAELNAILSLQGDSDDTQDITLKKKLLQMRFQSLSYQKAKHLLSGRDSKELDLPCQV 365

Query: 364  EDVELEIILVPTNAFIMGRPGSGKTAAMTVKLFMREQQQYIHPTGCSLVTRENAEVCYRN 423
            EDVELEIILVPTNAFIMGRPGSGKTAAMTVKLFMREQQQYIHPTGCSLVTRENAEVCYRN
Sbjct: 366  EDVELEIILVPTNAFIMGRPGSGKTAAMTVKLFMREQQQYIHPTGCSLVTRENAEVCYRN 425

Query: 424  EGGEECKKTERTVLRQLFITVTLKQCLDVKERLAYLKRISNGGNVFKETQNLCKADVLDM 483
            EGGEECKKTERTVLRQLFITVTLKQCL VKERLAYLKRISNGGNVFKETQNLCKADVLDM
Sbjct: 426  EGGEECKKTERTVLRQLFITVTLKQCLYVKERLAYLKRISNGGNVFKETQNLCKADVLDM 485

Query: 484  NDVQDLLDVPNSFDGIPANSFPLVITFRKFLIMLDRTVGDSYFIRFQKQWRLSGGKPKDS 543
            NDVQDLLDVPNSFDGIPANSFPLVITFRKFLIMLDRTVGDSYFIRFQKQWRLSGGKPKDS
Sbjct: 486  NDVQDLLDVPNSFDGIPANSFPLVITFRKFLIMLDRTVGDSYFIRFQKQWRLSGGKPKDS 545

Query: 544  LSRAAYNFIVSKEVTVKNFASSYWSYFDSCLTYKLDAVVVFNEIISQIKGGLGAKDALDG 603
            LSRAAYNFIVSKEVTVKNFASSYWSYFDSCLTYKLDAVVVFNEIISQIKGGLGAKDALDG
Sbjct: 546  LSRAAYNFIVSKEVTVKNFASSYWSYFDSCLTYKLDAVVVFNEIISQIKGGLGAKDALDG 605

Query: 604  RLSKLDYTRLSKGQSTLSRKQRERIYDIFLDYEKMKNAKGEYDLADLVIDLHRRLKVFQY 663
            +LSKLDYTRLSKGQSTLSRKQRERIYDIFLDYEKMKNAKGEYDLADLVIDLHRRLKVF+Y
Sbjct: 606  KLSKLDYTRLSKGQSTLSRKQRERIYDIFLDYEKMKNAKGEYDLADLVIDLHRRLKVFRY 665

Query: 664  TGDHMDFVYVDEVQALTMMQITLLKYLCKNVNSGFVFSSNTTQTIAKGIDFRFQDIRFLF 723
            TGDHMDFVYVDEVQALTMMQITLLKYLCKNVNSGFVFSSNTTQTIAKGIDFRFQDIRFLF
Sbjct: 666  TGDHMDFVYVDEVQALTMMQITLLKYLCKNVNSGFVFSSNTTQTIAKGIDFRFQDIRFLF 725

Query: 724  YKEFISGVKTDEKGIDAGLIKIPDILHINQNCRTQPKILQLANSVTDLLFRFFPRCIDIV 783
            YKEFISGVKTDEKGIDAGLIKIPDILHINQNCRTQPKILQLANSVTDLLFRFFPRCIDIV
Sbjct: 726  YKEFISGVKTDEKGIDAGLIKIPDILHINQNCRTQPKILQLANSVTDLLFRFFPRCIDIV 785

Query: 784  CPETSEMSSTDFETPVLLESRKGQNMMMVLFEEGRNIPADARGYGAKQVILVRDECARDE 843
            CPETSEMSSTDFETPVLLESRKGQNMMMVLFEEGRNIPADARGYGAKQVILVRDECARDE
Sbjct: 786  CPETSEMSSTDFETPVLLESRKGQNMMMVLFEEGRNIPADARGYGAKQVILVRDECARDE 845

Query: 844  ISSLVGNQAIIVTIMECQCMEFQDVLLYKFFNSSPLGNQWRVIYQYMIEQDMLEIAPGGS 903
            ISSLVGNQAIIVTIMECQCMEFQDVLLYKFFNSSPLGNQWRVIYQYMIEQDMLEIAPGGS
Sbjct: 846  ISSLVGNQAIIVTIMECQCMEFQDVLLYKFFNSSPLGNQWRVIYQYMIEQDMLEIAPGGS 905

Query: 904  PSFNQPVQLDLCWELKLLHIALTRSRRRLWIYEDNQEFSNPIVDYWKKLCYVQVKTLDYS 963
            PSFNQPVQLDLCWELKLLHIALTRSRRRLWIYEDNQEFSNPIVDYWKKLCYVQVKTLDYS
Sbjct: 906  PSFNQPVQLDLCWELKLLHIALTRSRRRLWIYEDNQEFSNPIVDYWKKLCYVQVKTLDYS 965

Query: 964  IAQTMKVPSTKEEWSSLGLEFFSEGVYGAASLCFERAEDRRRSEWARAASFCATANPQIS 1023
            I QTMKVPSTKEEWSSLGLEFFSEGVYGAASLCFERAEDRRRSEWARAASFCATANPQIS
Sbjct: 966  IVQTMKVPSTKEEWSSLGLEFFSEGVYGAASLCFERAEDRRRSEWARAASFCATANPQIS 1025

Query: 1024 RNALREAAEIYISLDRAEIAAKCYIELKEYKTAAYTYLTKCGEARLEDAGDCYMLAKCYK 1083
            RNALREAAEIYISLDRAEIAAKCYIELKEYKTAAYTYLTKCGEARLEDAGDCYMLAKCYK
Sbjct: 1026 RNALREAAEIYISLDRAEIAAKCYIELKEYKTAAYTYLTKCGEARLEDAGDCYMLAKCYK 1085

Query: 1084 LAAVAYSMGRCFLKFFDVCTAANLFDTGLQGICSWRKYDNVDLIKKCKHIKEAWHLFLWK 1143
            LAAVAYSMGRCFLKFFDVCTAANLFDTGLQGICSWRKYDNVDLIKKCKHIKEAWHLFLWK
Sbjct: 1086 LAAVAYSMGRCFLKFFDVCTAANLFDTGLQGICSWRKYDNVDLIKKCKHIKEAWHLFLWK 1145

Query: 1144 GALHYHQLQNFGSMMRFVESFDSIDEKYLFLGTLGLSENKMLQEEELTISENEGFHSPGL 1203
            GALHYHQLQNFGSMMRFVESFDSIDEKYLFLGTLGLSENKMLQEEELTISENEGFHSPGL
Sbjct: 1146 GALHYHQLQNFGSMMRFVESFDSIDEKYLFLGTLGLSENKMLQEEELTISENEGFHSPGL 1205

Query: 1204 HLQPKLVSVSVHKETSQNDTKTKGKMKVANNISTAKGSSRGSKFQPKLKSVWKETTFQND 1263
            HLQPKLVSVSVHKETSQNDTKTKGKMKVANNISTAKGSSRGSKFQPKLKSVWKETTFQND
Sbjct: 1206 HLQPKLVSVSVHKETSQNDTKTKGKMKVANNISTAKGSSRGSKFQPKLKSVWKETTFQND 1265

Query: 1264 TKSKERMKVADDMFSLGLQFQSKLEFKTVAQIDTTIRGKMKVAENMSTTKGSSQGLKFQS 1323
            TKSKERMKVADDMFSLGLQFQSKLEFKTVAQIDTTIRGKMKVAENMSTTKGSSQGLKFQS
Sbjct: 1266 TKSKERMKVADDMFSLGLQFQSKLEFKTVAQIDTTIRGKMKVAENMSTTKGSSQGLKFQS 1325

Query: 1324 KIKSVWKETTSQYNTMTKEVELADNLSTAEEPLQGLQFQCKLEFETISQNDTTTRDSMEV 1383
            KIKSVWKETTSQYNTMTKEVELADNLSTAEEPLQGLQFQCKLEFETISQNDTTTRDSMEV
Sbjct: 1326 KIKSVWKETTSQYNTMTKEVELADNLSTAEEPLQGLQFQCKLEFETISQNDTTTRDSMEV 1385

Query: 1384 SEDMSIVNGSSKELKFQPKLKSIWKETQSRNGTKTTDKMKLANSISMSIANESSQGLQFK 1443
            SEDMSIVNGSSKELKFQPKLKSIWKETQSRNGTKTTDKMKLANSISMSIANESSQGLQFK
Sbjct: 1386 SEDMSIVNGSSKELKFQPKLKSIWKETQSRNGTKTTDKMKLANSISMSIANESSQGLQFK 1445

Query: 1444 SKLKTKTVSKNDTEKKDKIQVAEIMSTSEWSSHGLQFQSNQESLCMEKTSQNDSKIEDNL 1503
            SKLKTKTVSKNDTEKKDKIQVAEIMSTSEWSSHGLQFQSNQESLCMEKTSQNDSKIEDNL
Sbjct: 1446 SKLKTKTVSKNDTEKKDKIQVAEIMSTSEWSSHGLQFQSNQESLCMEKTSQNDSKIEDNL 1505

Query: 1504 TVAPFISSPKDTSYKLQFKPKSVYAKEVIAAQNDLKMEKDEVNIVNKAEASQRLQQQCNQ 1563
            TVAPFISSPKDTSYKLQFKPKSVYAKEVIAAQNDLKMEKDEVNIVNKAEASQRLQQQCNQ
Sbjct: 1506 TVAPFISSPKDTSYKLQFKPKSVYAKEVIAAQNDLKMEKDEVNIVNKAEASQRLQQQCNQ 1565

Query: 1564 KVRNAHKETTSSIDSKAKKDKMKNSVNLSEFGDSSQQLQQLQIEQKKLKNKNVDGEKGKQ 1623
            KVRNAHKETTSSIDSKAKKDKMKNSVNLSEFGDSSQQLQQLQIEQKKLKNKNVDGEKGKQ
Sbjct: 1566 KVRNAHKETTSSIDSKAKKDKMKNSVNLSEFGDSSQQLQQLQIEQKKLKNKNVDGEKGKQ 1625

Query: 1624 KVTDHKFIAKQYWRKVTENGMKFNFQK 1650
            KVTDHKFIAKQYWRKVTENGMKFNFQK
Sbjct: 1626 KVTDHKFIAKQYWRKVTENGMKFNFQK 1652

BLAST of Cucsat.G18713.T3 vs. ExPASy TrEMBL
Match: A0A6J1KCZ4 (uncharacterized protein LOC111492119 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492119 PE=4 SV=1)

HSP 1 Score: 1880 bits (4871), Expect = 0.0
Identity = 951/1202 (79.12%), Postives = 1049/1202 (87.27%), Query Frame = 0

Query: 13   WRGAKTRLNVGVISFYAAQVSEIQSRLAHKYEKSHNFTVKVKSVDGFQGGEEDVIILTTV 72
            WR AKTRLNVGVISFYAAQVS IQSRL HKYEKS NFTVKVKSVDGFQGGEEDVIILTTV
Sbjct: 678  WRKAKTRLNVGVISFYAAQVSAIQSRLGHKYEKSDNFTVKVKSVDGFQGGEEDVIILTTV 737

Query: 73   RSNRRKNIGFISSSQRINVALTRARHCLWIVGDATTLGNSNSEWEAVVSDAKDRQCYFNA 132
            RSNRR NIGFIS+SQRINVALTRARHCLWIVGDATTLGNSNSEWE+VVS+AKDRQCYFNA
Sbjct: 738  RSNRRNNIGFISNSQRINVALTRARHCLWIVGDATTLGNSNSEWESVVSNAKDRQCYFNA 797

Query: 133  AEDKDFADAIIEVKKVLLELDDLLNKDSVLFTMAQWKVLLSDSFRASFQNVVSINQKKLI 192
             EDKD ADAIIEVKKVLLELDDLLN+DSVLF + QWKVLLSDSFRASFQ VVSINQKK I
Sbjct: 798  EEDKDLADAIIEVKKVLLELDDLLNQDSVLFKLVQWKVLLSDSFRASFQKVVSINQKKSI 857

Query: 193  LVLLLRLSCGWRPGTDYVPNLKCSNIIKCFKAEGLFIIYSLYIEKDLKYKQILKIWDIKP 252
            +VLLLRL+CGWRP  + V N KCSNII   K EGLFI+YSL IEKDLKYKQ+LKIWDIKP
Sbjct: 858  IVLLLRLACGWRPEANSVSNPKCSNIISV-KVEGLFIVYSLDIEKDLKYKQVLKIWDIKP 917

Query: 253  LTDVKVLVECLSDIHELYTDDFLNLCKAKSHKGDLELPITWSASPDIVVYKDYMKAELNA 312
            L DVKVLVECLS+IHELYTDDFLNLCKAKSHKGDLELPITW AS D+V+YKD+MKAEL+A
Sbjct: 918  LADVKVLVECLSNIHELYTDDFLNLCKAKSHKGDLELPITWGASLDVVIYKDHMKAELDA 977

Query: 313  ILSLQGDSDDTQDITLKKKLLQMRFQSLSYQKAKHLLSGRDSKELDLPCQVEDVELEIIL 372
            ILSLQ DSDD ++ TLKK LLQM+FQSLSY KAKHLLS   SKELDLPCQVED +LEIIL
Sbjct: 978  ILSLQADSDDIKNGTLKKNLLQMKFQSLSYLKAKHLLSRHASKELDLPCQVEDEQLEIIL 1037

Query: 373  VPTNAFIMGRPGSGKTAAMTVKLFMREQQQYIHPTGCSLVTRENAEVCYRNEGGEECKKT 432
             PT+AFIMGRP S KTAA+T+KLFMRE+QQ IH  GCS V R+NAEV YRN+GGE CKK 
Sbjct: 1038 FPTSAFIMGRPDSRKTAALTIKLFMRERQQQIHSGGCSQVMRDNAEVGYRNDGGEACKKI 1097

Query: 433  ERTVLRQLFITVTLKQCLDVKERLAYLKRISNGGNVFKETQNLCKADVLDMNDVQDLLDV 492
            +RTVLRQLFIT TLKQC  VKE L+YLKRISNGGN+ +E Q   K  V+DM+D QDLLDV
Sbjct: 1098 DRTVLRQLFITATLKQCQAVKEHLSYLKRISNGGNILEENQKFKKVGVMDMDDAQDLLDV 1157

Query: 493  PNSFDGIPANSFPLVITFRKFLIMLDRTVGDSYFIRFQKQWRLSGGKPKDSLSRAAYNFI 552
            PNSFDGIP +S+PLVITFRKFLIM+DRTVGDS+ IRF KQW+LS GKP+D LS AAYNFI
Sbjct: 1158 PNSFDGIPFSSYPLVITFRKFLIMVDRTVGDSFLIRFLKQWKLSCGKPRDPLSTAAYNFI 1217

Query: 553  VSKEVTVKNFASSYWSYFDSCLTYKLDAVVVFNEIISQIKGGLGAKDALDGRLSKLDYTR 612
            VSKEVTVK F+S YWSYFD CLT  LDAVVVFNEIISQIKGGLGAK+  DGRLSKLDYTR
Sbjct: 1218 VSKEVTVKKFSSFYWSYFDGCLTNNLDAVVVFNEIISQIKGGLGAKETPDGRLSKLDYTR 1277

Query: 613  LSKGQSTLSRKQRERIYDIFLDYEKMKNAKGEYDLADLVIDLHRRLKVFQYTGDHMDFVY 672
            L+KG+STLSRKQRERIYDIFLDYE+MKN KGEYDLADLVIDLH RLK  QYTGD MD+VY
Sbjct: 1278 LAKGRSTLSRKQRERIYDIFLDYERMKNEKGEYDLADLVIDLHHRLKCSQYTGDQMDYVY 1337

Query: 673  VDEVQALTMMQITLLKYLCKNVNSGFVFSSNTTQTIAKGIDFRFQDIRFLFYKEFISGVK 732
            VDEVQALTMM+I LLKYLC NV+SGFVFSSNT+QTIAKGIDFRF DIRFLFYKEFIS VK
Sbjct: 1338 VDEVQALTMMEIALLKYLCGNVSSGFVFSSNTSQTIAKGIDFRFHDIRFLFYKEFISRVK 1397

Query: 733  TDEKGIDAGLIKIPDILHINQNCRTQPKILQLANSVTDLLFRFFPRCIDIVCPETSEMSS 792
            TDEK I AGL+KIPDILH+NQNC TQPKILQLANSVTDLLFRFFP CIDI+CPETSEMSS
Sbjct: 1398 TDEKDIGAGLLKIPDILHMNQNCHTQPKILQLANSVTDLLFRFFPHCIDILCPETSEMSS 1457

Query: 793  TDFETPVLLESRKGQNMMMVLFEEGRNIPADARGYGAKQVILVRDECARDEISSLVGNQA 852
             +FETPVLLE+ KGQNMM +LF    N+PAD R +GAKQVILVRDE ARD IS+LV NQA
Sbjct: 1458 GNFETPVLLENGKGQNMMTLLFGGTGNVPADTREFGAKQVILVRDEHARDGISNLVRNQA 1517

Query: 853  IIVTIMECQCMEFQDVLLYKFFNSSPLGNQWRVIYQYMIEQDMLEIAPGGSPSFNQPVQL 912
            I++TIMECQ +EFQDVL+Y FFNSSPLG+QW VIYQYMIEQDMLE+AP  SP+FNQPV +
Sbjct: 1518 IVLTIMECQSLEFQDVLVYNFFNSSPLGHQWSVIYQYMIEQDMLEMAPN-SPNFNQPVHM 1577

Query: 913  DLCWELKLLHIALTRSRRRLWIYEDNQEFSNPIVDYWKKLCYVQVKTLDYSIAQTMKVPS 972
            DLCWELKLLHIA+TRSR+RLWIYEDNQEF NPIVDYWKKLCY+QVKTLDYSI Q MK PS
Sbjct: 1578 DLCWELKLLHIAITRSRQRLWIYEDNQEFPNPIVDYWKKLCYIQVKTLDYSIIQAMKAPS 1637

Query: 973  TKEEWSSLGLEFFSEGVYGAASLCFERAEDRRRSEWARAASFCATA------NPQISRNA 1032
            TKEEWSSLGLEFF EGVY AASLCFERA+DR + EWARAAS  ATA      NPQ++RNA
Sbjct: 1638 TKEEWSSLGLEFFCEGVYVAASLCFERADDRLKREWARAASLRATACILDGSNPQMARNA 1697

Query: 1033 LREAAEIYISLDRAEIAAKCYIELKEYKTAAYTYLTKCGEARLEDAGDCYMLAKCYKLAA 1092
            L+EAAEIYIS+DRAE+AAKC+IELKEY+TAAY Y  KCGEA+LEDAGDCYMLA+CY+LAA
Sbjct: 1698 LQEAAEIYISMDRAEVAAKCFIELKEYQTAAYIYSKKCGEAKLEDAGDCYMLAECYELAA 1757

Query: 1093 VAYSMGRCFLKFFDVCTAANLFDTGLQGICSWRKY--DNVDLIKKCKHIKEAWHLFLWKG 1152
             AYS GR FLKF +VCT ANLFD GLQ ICSWRK+  D+ DLI+KC   KE WH+FL KG
Sbjct: 1758 EAYSRGRFFLKFLNVCTVANLFDMGLQVICSWRKHCDDDDDLIEKCLDFKEIWHVFLQKG 1817

Query: 1153 ALHYHQLQNFGSMMRFVESFDSIDEKYLFLGTLGLSENKMLQEEELTISENEGFHSPGLH 1206
            ALHYH+LQ+F S+++F + FDS+DEK  FL TLGLSE  +L E+++  + +      G+ 
Sbjct: 1818 ALHYHELQDFRSILKFFDIFDSMDEKCSFLRTLGLSEKILLLEKDVEDATSIIMKKEGIS 1877

BLAST of Cucsat.G18713.T3 vs. ExPASy TrEMBL
Match: A0A6J1KCY2 (uncharacterized protein LOC111492119 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492119 PE=4 SV=1)

HSP 1 Score: 1880 bits (4871), Expect = 0.0
Identity = 951/1202 (79.12%), Postives = 1049/1202 (87.27%), Query Frame = 0

Query: 13   WRGAKTRLNVGVISFYAAQVSEIQSRLAHKYEKSHNFTVKVKSVDGFQGGEEDVIILTTV 72
            WR AKTRLNVGVISFYAAQVS IQSRL HKYEKS NFTVKVKSVDGFQGGEEDVIILTTV
Sbjct: 717  WRKAKTRLNVGVISFYAAQVSAIQSRLGHKYEKSDNFTVKVKSVDGFQGGEEDVIILTTV 776

Query: 73   RSNRRKNIGFISSSQRINVALTRARHCLWIVGDATTLGNSNSEWEAVVSDAKDRQCYFNA 132
            RSNRR NIGFIS+SQRINVALTRARHCLWIVGDATTLGNSNSEWE+VVS+AKDRQCYFNA
Sbjct: 777  RSNRRNNIGFISNSQRINVALTRARHCLWIVGDATTLGNSNSEWESVVSNAKDRQCYFNA 836

Query: 133  AEDKDFADAIIEVKKVLLELDDLLNKDSVLFTMAQWKVLLSDSFRASFQNVVSINQKKLI 192
             EDKD ADAIIEVKKVLLELDDLLN+DSVLF + QWKVLLSDSFRASFQ VVSINQKK I
Sbjct: 837  EEDKDLADAIIEVKKVLLELDDLLNQDSVLFKLVQWKVLLSDSFRASFQKVVSINQKKSI 896

Query: 193  LVLLLRLSCGWRPGTDYVPNLKCSNIIKCFKAEGLFIIYSLYIEKDLKYKQILKIWDIKP 252
            +VLLLRL+CGWRP  + V N KCSNII   K EGLFI+YSL IEKDLKYKQ+LKIWDIKP
Sbjct: 897  IVLLLRLACGWRPEANSVSNPKCSNIISV-KVEGLFIVYSLDIEKDLKYKQVLKIWDIKP 956

Query: 253  LTDVKVLVECLSDIHELYTDDFLNLCKAKSHKGDLELPITWSASPDIVVYKDYMKAELNA 312
            L DVKVLVECLS+IHELYTDDFLNLCKAKSHKGDLELPITW AS D+V+YKD+MKAEL+A
Sbjct: 957  LADVKVLVECLSNIHELYTDDFLNLCKAKSHKGDLELPITWGASLDVVIYKDHMKAELDA 1016

Query: 313  ILSLQGDSDDTQDITLKKKLLQMRFQSLSYQKAKHLLSGRDSKELDLPCQVEDVELEIIL 372
            ILSLQ DSDD ++ TLKK LLQM+FQSLSY KAKHLLS   SKELDLPCQVED +LEIIL
Sbjct: 1017 ILSLQADSDDIKNGTLKKNLLQMKFQSLSYLKAKHLLSRHASKELDLPCQVEDEQLEIIL 1076

Query: 373  VPTNAFIMGRPGSGKTAAMTVKLFMREQQQYIHPTGCSLVTRENAEVCYRNEGGEECKKT 432
             PT+AFIMGRP S KTAA+T+KLFMRE+QQ IH  GCS V R+NAEV YRN+GGE CKK 
Sbjct: 1077 FPTSAFIMGRPDSRKTAALTIKLFMRERQQQIHSGGCSQVMRDNAEVGYRNDGGEACKKI 1136

Query: 433  ERTVLRQLFITVTLKQCLDVKERLAYLKRISNGGNVFKETQNLCKADVLDMNDVQDLLDV 492
            +RTVLRQLFIT TLKQC  VKE L+YLKRISNGGN+ +E Q   K  V+DM+D QDLLDV
Sbjct: 1137 DRTVLRQLFITATLKQCQAVKEHLSYLKRISNGGNILEENQKFKKVGVMDMDDAQDLLDV 1196

Query: 493  PNSFDGIPANSFPLVITFRKFLIMLDRTVGDSYFIRFQKQWRLSGGKPKDSLSRAAYNFI 552
            PNSFDGIP +S+PLVITFRKFLIM+DRTVGDS+ IRF KQW+LS GKP+D LS AAYNFI
Sbjct: 1197 PNSFDGIPFSSYPLVITFRKFLIMVDRTVGDSFLIRFLKQWKLSCGKPRDPLSTAAYNFI 1256

Query: 553  VSKEVTVKNFASSYWSYFDSCLTYKLDAVVVFNEIISQIKGGLGAKDALDGRLSKLDYTR 612
            VSKEVTVK F+S YWSYFD CLT  LDAVVVFNEIISQIKGGLGAK+  DGRLSKLDYTR
Sbjct: 1257 VSKEVTVKKFSSFYWSYFDGCLTNNLDAVVVFNEIISQIKGGLGAKETPDGRLSKLDYTR 1316

Query: 613  LSKGQSTLSRKQRERIYDIFLDYEKMKNAKGEYDLADLVIDLHRRLKVFQYTGDHMDFVY 672
            L+KG+STLSRKQRERIYDIFLDYE+MKN KGEYDLADLVIDLH RLK  QYTGD MD+VY
Sbjct: 1317 LAKGRSTLSRKQRERIYDIFLDYERMKNEKGEYDLADLVIDLHHRLKCSQYTGDQMDYVY 1376

Query: 673  VDEVQALTMMQITLLKYLCKNVNSGFVFSSNTTQTIAKGIDFRFQDIRFLFYKEFISGVK 732
            VDEVQALTMM+I LLKYLC NV+SGFVFSSNT+QTIAKGIDFRF DIRFLFYKEFIS VK
Sbjct: 1377 VDEVQALTMMEIALLKYLCGNVSSGFVFSSNTSQTIAKGIDFRFHDIRFLFYKEFISRVK 1436

Query: 733  TDEKGIDAGLIKIPDILHINQNCRTQPKILQLANSVTDLLFRFFPRCIDIVCPETSEMSS 792
            TDEK I AGL+KIPDILH+NQNC TQPKILQLANSVTDLLFRFFP CIDI+CPETSEMSS
Sbjct: 1437 TDEKDIGAGLLKIPDILHMNQNCHTQPKILQLANSVTDLLFRFFPHCIDILCPETSEMSS 1496

Query: 793  TDFETPVLLESRKGQNMMMVLFEEGRNIPADARGYGAKQVILVRDECARDEISSLVGNQA 852
             +FETPVLLE+ KGQNMM +LF    N+PAD R +GAKQVILVRDE ARD IS+LV NQA
Sbjct: 1497 GNFETPVLLENGKGQNMMTLLFGGTGNVPADTREFGAKQVILVRDEHARDGISNLVRNQA 1556

Query: 853  IIVTIMECQCMEFQDVLLYKFFNSSPLGNQWRVIYQYMIEQDMLEIAPGGSPSFNQPVQL 912
            I++TIMECQ +EFQDVL+Y FFNSSPLG+QW VIYQYMIEQDMLE+AP  SP+FNQPV +
Sbjct: 1557 IVLTIMECQSLEFQDVLVYNFFNSSPLGHQWSVIYQYMIEQDMLEMAPN-SPNFNQPVHM 1616

Query: 913  DLCWELKLLHIALTRSRRRLWIYEDNQEFSNPIVDYWKKLCYVQVKTLDYSIAQTMKVPS 972
            DLCWELKLLHIA+TRSR+RLWIYEDNQEF NPIVDYWKKLCY+QVKTLDYSI Q MK PS
Sbjct: 1617 DLCWELKLLHIAITRSRQRLWIYEDNQEFPNPIVDYWKKLCYIQVKTLDYSIIQAMKAPS 1676

Query: 973  TKEEWSSLGLEFFSEGVYGAASLCFERAEDRRRSEWARAASFCATA------NPQISRNA 1032
            TKEEWSSLGLEFF EGVY AASLCFERA+DR + EWARAAS  ATA      NPQ++RNA
Sbjct: 1677 TKEEWSSLGLEFFCEGVYVAASLCFERADDRLKREWARAASLRATACILDGSNPQMARNA 1736

Query: 1033 LREAAEIYISLDRAEIAAKCYIELKEYKTAAYTYLTKCGEARLEDAGDCYMLAKCYKLAA 1092
            L+EAAEIYIS+DRAE+AAKC+IELKEY+TAAY Y  KCGEA+LEDAGDCYMLA+CY+LAA
Sbjct: 1737 LQEAAEIYISMDRAEVAAKCFIELKEYQTAAYIYSKKCGEAKLEDAGDCYMLAECYELAA 1796

Query: 1093 VAYSMGRCFLKFFDVCTAANLFDTGLQGICSWRKY--DNVDLIKKCKHIKEAWHLFLWKG 1152
             AYS GR FLKF +VCT ANLFD GLQ ICSWRK+  D+ DLI+KC   KE WH+FL KG
Sbjct: 1797 EAYSRGRFFLKFLNVCTVANLFDMGLQVICSWRKHCDDDDDLIEKCLDFKEIWHVFLQKG 1856

Query: 1153 ALHYHQLQNFGSMMRFVESFDSIDEKYLFLGTLGLSENKMLQEEELTISENEGFHSPGLH 1206
            ALHYH+LQ+F S+++F + FDS+DEK  FL TLGLSE  +L E+++  + +      G+ 
Sbjct: 1857 ALHYHELQDFRSILKFFDIFDSMDEKCSFLRTLGLSEKILLLEKDVEDATSIIMKKEGIS 1916

BLAST of Cucsat.G18713.T3 vs. ExPASy TrEMBL
Match: A0A6J1GWV9 (uncharacterized protein LOC111458260 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458260 PE=4 SV=1)

HSP 1 Score: 1879 bits (4868), Expect = 0.0
Identity = 954/1202 (79.37%), Postives = 1046/1202 (87.02%), Query Frame = 0

Query: 13   WRGAKTRLNVGVISFYAAQVSEIQSRLAHKYEKSHNFTVKVKSVDGFQGGEEDVIILTTV 72
            WR AKTRLNVGVISFYAAQVS IQSRL HKYEKS NFTVKVKSVDGFQGGEEDVIILTTV
Sbjct: 717  WRKAKTRLNVGVISFYAAQVSAIQSRLGHKYEKSDNFTVKVKSVDGFQGGEEDVIILTTV 776

Query: 73   RSNRRKNIGFISSSQRINVALTRARHCLWIVGDATTLGNSNSEWEAVVSDAKDRQCYFNA 132
            RSNRR NIGFIS+SQRINVALTRARHCLWIVGDATTLGNSNSEWE+VVS+AKDRQCYFNA
Sbjct: 777  RSNRRNNIGFISNSQRINVALTRARHCLWIVGDATTLGNSNSEWESVVSNAKDRQCYFNA 836

Query: 133  AEDKDFADAIIEVKKVLLELDDLLNKDSVLFTMAQWKVLLSDSFRASFQNVVSINQKKLI 192
             EDKD ADAIIEVKKVLLELDDLLNKDSVLF + QWKVLLSDSFRASFQ +VSINQKK I
Sbjct: 837  EEDKDLADAIIEVKKVLLELDDLLNKDSVLFKLVQWKVLLSDSFRASFQKLVSINQKKSI 896

Query: 193  LVLLLRLSCGWRPGTDYVPNLKCSNIIKCFKAEGLFIIYSLYIEKDLKYKQILKIWDIKP 252
            +VLLLRL+CGWRP  + V N KCSNII  FK EGLFI+YSL IEKD KYKQ+LKIWDIKP
Sbjct: 897  IVLLLRLACGWRPEANSVSNTKCSNIIS-FKVEGLFIVYSLDIEKDSKYKQVLKIWDIKP 956

Query: 253  LTDVKVLVECLSDIHELYTDDFLNLCKAKSHKGDLELPITWSASPDIVVYKDYMKAELNA 312
            L DVKVLVECLS+IHELYTDDFLNLCKAKSHKGDLELPITWSAS D+V+YKD+MKAEL+A
Sbjct: 957  LADVKVLVECLSNIHELYTDDFLNLCKAKSHKGDLELPITWSASLDVVMYKDHMKAELDA 1016

Query: 313  ILSLQGDSDDTQDITLKKKLLQMRFQSLSYQKAKHLLSGRDSKELDLPCQVEDVELEIIL 372
            ILSLQ DSDD ++ TLKK LLQM+FQSLSY KAK+LLS  DSKELDLPCQVED +LEIIL
Sbjct: 1017 ILSLQADSDDIKNSTLKKNLLQMKFQSLSYLKAKYLLSRHDSKELDLPCQVEDEQLEIIL 1076

Query: 373  VPTNAFIMGRPGSGKTAAMTVKLFMREQQQYIHPTGCSLVTRENAEVCYRNEGGEECKKT 432
             PT+AFIMGRP SGKTAA+T+KLFMREQQQ IH  GCS VT ENAEV YRN+GGE CKK 
Sbjct: 1077 FPTSAFIMGRPDSGKTAALTMKLFMREQQQQIHSAGCSQVTIENAEVGYRNDGGEACKKI 1136

Query: 433  ERTVLRQLFITVTLKQCLDVKERLAYLKRISNGGNVFKETQNLCKADVLDMNDVQDLLDV 492
            +R VLRQLFIT +LK C  VKE L+YLKRIS GGN+ +E Q   K   +DM+D QDLLDV
Sbjct: 1137 DRIVLRQLFITASLKHCQAVKEHLSYLKRISTGGNLLEENQKFNKVGAMDMDDAQDLLDV 1196

Query: 493  PNSFDGIPANSFPLVITFRKFLIMLDRTVGDSYFIRFQKQWRLSGGKPKDSLSRAAYNFI 552
            PNSFDGIP +S+PLVITFRKFLIM+DRTVGDS+ +RF KQW+LS GKP+D LS AAYNFI
Sbjct: 1197 PNSFDGIPFSSYPLVITFRKFLIMVDRTVGDSFLVRFLKQWKLSCGKPRDPLSTAAYNFI 1256

Query: 553  VSKEVTVKNFASSYWSYFDSCLTYKLDAVVVFNEIISQIKGGLGAKDALDGRLSKLDYTR 612
            VSKEVTVKNFASSYWSYFD  LT  LDAVVVFNEIISQIKGGLGAK+  DGRLSKLDYTR
Sbjct: 1257 VSKEVTVKNFASSYWSYFDGRLTNNLDAVVVFNEIISQIKGGLGAKETPDGRLSKLDYTR 1316

Query: 613  LSKGQSTLSRKQRERIYDIFLDYEKMKNAKGEYDLADLVIDLHRRLKVFQYTGDHMDFVY 672
            L+KG+STLSRKQRERIYDIFLDYE+MKN KGEYDLADLVIDLH RLK  QYTGD MD+VY
Sbjct: 1317 LAKGRSTLSRKQRERIYDIFLDYERMKNEKGEYDLADLVIDLHHRLKCSQYTGDQMDYVY 1376

Query: 673  VDEVQALTMMQITLLKYLCKNVNSGFVFSSNTTQTIAKGIDFRFQDIRFLFYKEFISGVK 732
            VDEVQALTMM+I LLKYLC NV+SGFVFSSNT QTIAKGIDFRF DIRFLFYKEFIS VK
Sbjct: 1377 VDEVQALTMMEIALLKYLCGNVSSGFVFSSNTAQTIAKGIDFRFHDIRFLFYKEFISRVK 1436

Query: 733  TDEKGIDAGLIKIPDILHINQNCRTQPKILQLANSVTDLLFRFFPRCIDIVCPETSEMSS 792
             DEK I AGL+KIPDILH+NQNC TQPKILQLA+SVTDLLFRFFP CIDI+CPETSEMSS
Sbjct: 1437 ADEKDIGAGLLKIPDILHMNQNCHTQPKILQLASSVTDLLFRFFPHCIDILCPETSEMSS 1496

Query: 793  TDFETPVLLESRKGQNMMMVLFEEGRNIPADARGYGAKQVILVRDECARDEISSLVGNQA 852
             +FETPVLLE+ KGQNMM +LF    NIPAD R +GAKQVILVRDE ARD IS+LV NQA
Sbjct: 1497 GNFETPVLLENGKGQNMMTLLFGGTGNIPADTREFGAKQVILVRDEHARDGISNLVRNQA 1556

Query: 853  IIVTIMECQCMEFQDVLLYKFFNSSPLGNQWRVIYQYMIEQDMLEIAPGGSPSFNQPVQL 912
            I++TIMECQ +EFQDVLLY FFNSSPLG+QW VIYQYMIEQDMLE+AP  SP+FNQPV +
Sbjct: 1557 IVLTIMECQSLEFQDVLLYNFFNSSPLGHQWSVIYQYMIEQDMLEMAPN-SPNFNQPVHM 1616

Query: 913  DLCWELKLLHIALTRSRRRLWIYEDNQEFSNPIVDYWKKLCYVQVKTLDYSIAQTMKVPS 972
            DLCWELKLLHIA+TRSR+RLWIYEDNQEF NPIVDYWKKLCY+QVKTLDYSI Q MK PS
Sbjct: 1617 DLCWELKLLHIAITRSRQRLWIYEDNQEFPNPIVDYWKKLCYIQVKTLDYSIIQAMKAPS 1676

Query: 973  TKEEWSSLGLEFFSEGVYGAASLCFERAEDRRRSEWARAASFCATA------NPQISRNA 1032
            TKEEWSSLGLEFF EGVY AASLCFERA+DR R EWARAAS  ATA      NPQ++RNA
Sbjct: 1677 TKEEWSSLGLEFFCEGVYVAASLCFERADDRLRREWARAASLRATACILDGSNPQMARNA 1736

Query: 1033 LREAAEIYISLDRAEIAAKCYIELKEYKTAAYTYLTKCGEARLEDAGDCYMLAKCYKLAA 1092
            L+EAAEIYIS+DRAE+AAKC+IELKEY+TAAY Y  KCGEA+LEDAGDCYMLA+CY+LAA
Sbjct: 1737 LQEAAEIYISMDRAEVAAKCFIELKEYQTAAYIYSKKCGEAKLEDAGDCYMLAECYELAA 1796

Query: 1093 VAYSMGRCFLKFFDVCTAANLFDTGLQGICSWRKY--DNVDLIKKCKHIKEAWHLFLWKG 1152
             AYS GR FLKF +VCT ANLFD GLQ ICSWRK+   + DLI+KC   KE WH+FL KG
Sbjct: 1797 EAYSRGRFFLKFLNVCTVANLFDMGLQVICSWRKHCDHDDDLIEKCLDFKEIWHVFLQKG 1856

Query: 1153 ALHYHQLQNFGSMMRFVESFDSIDEKYLFLGTLGLSENKMLQEEELTISENEGFHSPGLH 1206
            ALHYHQLQ+F S+++FV+ FDS+DEK  FL TLGLSE  +L E+++    N      G+ 
Sbjct: 1857 ALHYHQLQDFRSILKFVDIFDSMDEKCSFLRTLGLSEKILLLEKDVEEDTNIIMKKEGIL 1916

BLAST of Cucsat.G18713.T3 vs. ExPASy TrEMBL
Match: A0A6J1GWN5 (uncharacterized protein LOC111458260 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111458260 PE=4 SV=1)

HSP 1 Score: 1879 bits (4868), Expect = 0.0
Identity = 954/1202 (79.37%), Postives = 1046/1202 (87.02%), Query Frame = 0

Query: 13   WRGAKTRLNVGVISFYAAQVSEIQSRLAHKYEKSHNFTVKVKSVDGFQGGEEDVIILTTV 72
            WR AKTRLNVGVISFYAAQVS IQSRL HKYEKS NFTVKVKSVDGFQGGEEDVIILTTV
Sbjct: 678  WRKAKTRLNVGVISFYAAQVSAIQSRLGHKYEKSDNFTVKVKSVDGFQGGEEDVIILTTV 737

Query: 73   RSNRRKNIGFISSSQRINVALTRARHCLWIVGDATTLGNSNSEWEAVVSDAKDRQCYFNA 132
            RSNRR NIGFIS+SQRINVALTRARHCLWIVGDATTLGNSNSEWE+VVS+AKDRQCYFNA
Sbjct: 738  RSNRRNNIGFISNSQRINVALTRARHCLWIVGDATTLGNSNSEWESVVSNAKDRQCYFNA 797

Query: 133  AEDKDFADAIIEVKKVLLELDDLLNKDSVLFTMAQWKVLLSDSFRASFQNVVSINQKKLI 192
             EDKD ADAIIEVKKVLLELDDLLNKDSVLF + QWKVLLSDSFRASFQ +VSINQKK I
Sbjct: 798  EEDKDLADAIIEVKKVLLELDDLLNKDSVLFKLVQWKVLLSDSFRASFQKLVSINQKKSI 857

Query: 193  LVLLLRLSCGWRPGTDYVPNLKCSNIIKCFKAEGLFIIYSLYIEKDLKYKQILKIWDIKP 252
            +VLLLRL+CGWRP  + V N KCSNII  FK EGLFI+YSL IEKD KYKQ+LKIWDIKP
Sbjct: 858  IVLLLRLACGWRPEANSVSNTKCSNIIS-FKVEGLFIVYSLDIEKDSKYKQVLKIWDIKP 917

Query: 253  LTDVKVLVECLSDIHELYTDDFLNLCKAKSHKGDLELPITWSASPDIVVYKDYMKAELNA 312
            L DVKVLVECLS+IHELYTDDFLNLCKAKSHKGDLELPITWSAS D+V+YKD+MKAEL+A
Sbjct: 918  LADVKVLVECLSNIHELYTDDFLNLCKAKSHKGDLELPITWSASLDVVMYKDHMKAELDA 977

Query: 313  ILSLQGDSDDTQDITLKKKLLQMRFQSLSYQKAKHLLSGRDSKELDLPCQVEDVELEIIL 372
            ILSLQ DSDD ++ TLKK LLQM+FQSLSY KAK+LLS  DSKELDLPCQVED +LEIIL
Sbjct: 978  ILSLQADSDDIKNSTLKKNLLQMKFQSLSYLKAKYLLSRHDSKELDLPCQVEDEQLEIIL 1037

Query: 373  VPTNAFIMGRPGSGKTAAMTVKLFMREQQQYIHPTGCSLVTRENAEVCYRNEGGEECKKT 432
             PT+AFIMGRP SGKTAA+T+KLFMREQQQ IH  GCS VT ENAEV YRN+GGE CKK 
Sbjct: 1038 FPTSAFIMGRPDSGKTAALTMKLFMREQQQQIHSAGCSQVTIENAEVGYRNDGGEACKKI 1097

Query: 433  ERTVLRQLFITVTLKQCLDVKERLAYLKRISNGGNVFKETQNLCKADVLDMNDVQDLLDV 492
            +R VLRQLFIT +LK C  VKE L+YLKRIS GGN+ +E Q   K   +DM+D QDLLDV
Sbjct: 1098 DRIVLRQLFITASLKHCQAVKEHLSYLKRISTGGNLLEENQKFNKVGAMDMDDAQDLLDV 1157

Query: 493  PNSFDGIPANSFPLVITFRKFLIMLDRTVGDSYFIRFQKQWRLSGGKPKDSLSRAAYNFI 552
            PNSFDGIP +S+PLVITFRKFLIM+DRTVGDS+ +RF KQW+LS GKP+D LS AAYNFI
Sbjct: 1158 PNSFDGIPFSSYPLVITFRKFLIMVDRTVGDSFLVRFLKQWKLSCGKPRDPLSTAAYNFI 1217

Query: 553  VSKEVTVKNFASSYWSYFDSCLTYKLDAVVVFNEIISQIKGGLGAKDALDGRLSKLDYTR 612
            VSKEVTVKNFASSYWSYFD  LT  LDAVVVFNEIISQIKGGLGAK+  DGRLSKLDYTR
Sbjct: 1218 VSKEVTVKNFASSYWSYFDGRLTNNLDAVVVFNEIISQIKGGLGAKETPDGRLSKLDYTR 1277

Query: 613  LSKGQSTLSRKQRERIYDIFLDYEKMKNAKGEYDLADLVIDLHRRLKVFQYTGDHMDFVY 672
            L+KG+STLSRKQRERIYDIFLDYE+MKN KGEYDLADLVIDLH RLK  QYTGD MD+VY
Sbjct: 1278 LAKGRSTLSRKQRERIYDIFLDYERMKNEKGEYDLADLVIDLHHRLKCSQYTGDQMDYVY 1337

Query: 673  VDEVQALTMMQITLLKYLCKNVNSGFVFSSNTTQTIAKGIDFRFQDIRFLFYKEFISGVK 732
            VDEVQALTMM+I LLKYLC NV+SGFVFSSNT QTIAKGIDFRF DIRFLFYKEFIS VK
Sbjct: 1338 VDEVQALTMMEIALLKYLCGNVSSGFVFSSNTAQTIAKGIDFRFHDIRFLFYKEFISRVK 1397

Query: 733  TDEKGIDAGLIKIPDILHINQNCRTQPKILQLANSVTDLLFRFFPRCIDIVCPETSEMSS 792
             DEK I AGL+KIPDILH+NQNC TQPKILQLA+SVTDLLFRFFP CIDI+CPETSEMSS
Sbjct: 1398 ADEKDIGAGLLKIPDILHMNQNCHTQPKILQLASSVTDLLFRFFPHCIDILCPETSEMSS 1457

Query: 793  TDFETPVLLESRKGQNMMMVLFEEGRNIPADARGYGAKQVILVRDECARDEISSLVGNQA 852
             +FETPVLLE+ KGQNMM +LF    NIPAD R +GAKQVILVRDE ARD IS+LV NQA
Sbjct: 1458 GNFETPVLLENGKGQNMMTLLFGGTGNIPADTREFGAKQVILVRDEHARDGISNLVRNQA 1517

Query: 853  IIVTIMECQCMEFQDVLLYKFFNSSPLGNQWRVIYQYMIEQDMLEIAPGGSPSFNQPVQL 912
            I++TIMECQ +EFQDVLLY FFNSSPLG+QW VIYQYMIEQDMLE+AP  SP+FNQPV +
Sbjct: 1518 IVLTIMECQSLEFQDVLLYNFFNSSPLGHQWSVIYQYMIEQDMLEMAPN-SPNFNQPVHM 1577

Query: 913  DLCWELKLLHIALTRSRRRLWIYEDNQEFSNPIVDYWKKLCYVQVKTLDYSIAQTMKVPS 972
            DLCWELKLLHIA+TRSR+RLWIYEDNQEF NPIVDYWKKLCY+QVKTLDYSI Q MK PS
Sbjct: 1578 DLCWELKLLHIAITRSRQRLWIYEDNQEFPNPIVDYWKKLCYIQVKTLDYSIIQAMKAPS 1637

Query: 973  TKEEWSSLGLEFFSEGVYGAASLCFERAEDRRRSEWARAASFCATA------NPQISRNA 1032
            TKEEWSSLGLEFF EGVY AASLCFERA+DR R EWARAAS  ATA      NPQ++RNA
Sbjct: 1638 TKEEWSSLGLEFFCEGVYVAASLCFERADDRLRREWARAASLRATACILDGSNPQMARNA 1697

Query: 1033 LREAAEIYISLDRAEIAAKCYIELKEYKTAAYTYLTKCGEARLEDAGDCYMLAKCYKLAA 1092
            L+EAAEIYIS+DRAE+AAKC+IELKEY+TAAY Y  KCGEA+LEDAGDCYMLA+CY+LAA
Sbjct: 1698 LQEAAEIYISMDRAEVAAKCFIELKEYQTAAYIYSKKCGEAKLEDAGDCYMLAECYELAA 1757

Query: 1093 VAYSMGRCFLKFFDVCTAANLFDTGLQGICSWRKY--DNVDLIKKCKHIKEAWHLFLWKG 1152
             AYS GR FLKF +VCT ANLFD GLQ ICSWRK+   + DLI+KC   KE WH+FL KG
Sbjct: 1758 EAYSRGRFFLKFLNVCTVANLFDMGLQVICSWRKHCDHDDDLIEKCLDFKEIWHVFLQKG 1817

Query: 1153 ALHYHQLQNFGSMMRFVESFDSIDEKYLFLGTLGLSENKMLQEEELTISENEGFHSPGLH 1206
            ALHYHQLQ+F S+++FV+ FDS+DEK  FL TLGLSE  +L E+++    N      G+ 
Sbjct: 1818 ALHYHQLQDFRSILKFVDIFDSMDEKCSFLRTLGLSEKILLLEKDVEEDTNIIMKKEGIL 1877