Cucsat.G18488.T1 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G18488.T1
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionNADPH--cytochrome P450 reductase
Locationctg3379: 2626546 .. 2631883 (+)
RNA-Seq ExpressionCucsat.G18488.T1
SyntenyCucsat.G18488.T1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TGACTGGTCGGGTTTGGTAGGCGTTGGTTCATTCACGCGCTTCTTTGCCCTTCCAATATAAACACACCCCTTCTCTTATTTCTTCTCTCTCAACGCCAACTCACAACCACCACCTCTTCTGCCACCGCCTTCCTCCACCGGAGTTCCGGCCTATTCTTCACTGCATCTATTCACATTTTTCACTCCATACTGTTACTCCCATTCTTAACCGGATTCCGGTGGTTTCTTAATTCCTTGGTCATATCCTCCACCGGATTCCAGTGAGGTTTTTTAAGTTTCTTTGGATTCGGACTCCATCATGCAATCGGAATCCAGTTCTATGAAGGCTTCTCCATTTGACTTCATGTCGGCTATAATTAAGGGCAGGATGGATCCGTCTAATTCTTCATTTCAATCGACTGGCGAGGGTGCCTCAGTTATTTTCGAGAATCGCGAGCTGGTTGCGATCTTAACTACCTCGATCGCTGTCATGATTGGCTGCTTTGTTGTTCTTGTGTGGCGAAGATCCGGAAATCGAAAAGTTAAGACTATAGAGCTTCCTAAGCCGTTGCTTGGGAAGGAGCCAGAGCCAGAAGTTGACGACGGGAAGAAGAAGGTTACGATATTCTTTGGTACGCAGACTGGTACTGCTGAAGGCTTTGCAAAGGTTTGCATGTGAAAACAATCGTAGGCGTTGAGAATTTTGTGTAACTCGTTCGTTTGTTTACTTTTTTTTTTTTTTTTTTTGGCTATTGCTTACGAGTGACTGCGATTGTTCTCAGGCTCTATCTGACGAGGCGAAAGCACGGTACGATAAGGCCAAGTTTAGAGTTGTTGATTTGGTAAGCTTCTTTTTCTTATTTTGTATCTGTGATGTTGGTGGATGGCATTTTGGCACAGTCTAGTTTTGATTGACGAACTATGGTGCTGACATTTCGAATTGGACCTTCTTGCGCAGGATGATTATGGGGCTGACGAAGATGAATACGAACAAAAATTGAAAAAGGAGTCTGTAGCTGTTTTCTTCTTGGCAACGTGAGTTTCAGCATACGCGCAAACTTTCAAACTTTTGTTTGGATCCCTCATCTTTATTTCAAGGTTTAAGTCGTCTGTTTTTAGTTTCTTTTAGTGGTTAATTGTTTATGAATGCAGGTATGGCGATGGAGAGCCCACTGATAATGCCGCAAGATTCTATAAATGGTTCACCGAGGTAAAACTTCTTTCGGCATATTTCGTTTTGTGCAAATCATTAGAATTGTTTTTCTGATCTAAATATGCATATTCATAGGGTAAAGAGAGAGGGGAATGTCTTCAGAACCTCAATTATGCAGTCTTTGGCCTTGGCAACCGACAATATGAGCATTTTAATAAGGTCTCAATGGTTCCTTCCAAATTTCTTGGCTTCAATTGTTGATTGTTGATTGTCCGTTGTCTGATTCAGATTCTGCTATGCTAACTAGATTCAACTTTTTTTTTTGGATGGTGACAAACAGATTGCAAAAGTGGTTGATGAGCTGCTTGAGACTCAGGGTATGCTCTTGCCTTCTTTCTGATGTTCATTTATTAGGAAACCGCAATGATAAAATTTCTTTGAGAACACGATTAAGCGAAGATTAATGCATCTTTGAAGATAGTTTTTATAAGTTTATTACGCTTGCCAATATAATTCTTTATTTTGTTTGCAGGTGGTAAGCGCCTTGTAAAAGTTGGACTTGGAGATGACGATCAGTGCATAGAGGATGACTTCTCTGCTTGGTATGGAGAAAGTTGATTTGTTTGCTGAGGTTTGTTAAAGAAGATTGGCAACTCATACTTTCATGGTTTACTAATTTTTTTAGGCGAGAATCATTGTGGCCTGAGTTGGATCAATTGCTTCGGGATGAGGATGATGCAGCAACTGTGACCACACCTTACACAGCTGCCATATCAGAATACCGAGTGGTATTCCATGATCCTTCAGATGTAACTGATGACAAAAAGAACTGGATGAATGCAAATGGTCATGCTGTACATGACGCACAACATCCATTCAGGTTGTGAGAAATTTGATGTGTTGTGTTTTGAGTTTAACTTGCACCTGTCTTTACCCATCTGGAAAATTTAGAAATAAGGAAAAGAAGTAGAATGAAAATCTAACTTATTGTTTTATTGCATGAAGATCTAATGTGGTTGTGAGAAAGGAGCTCCATACACCTGCGTCTGATCGTTCTTGTACTCATCTAGAGTTTGATATTTCTGAGTCTGCACTCAAGTAAGTAGCCTAGTTAATTGTTGGGATTGTTCTATATGTCTGTACTATAATTTAGTAGCATAACACCATTCTCTAACGTTTGTTCTCTTTCACCAGATATGAAACAGGGGATCATGTTGGTGTTTACTGTGAAAATTTAACCGAGACTGTTGATGAGGCTCTAAATTTATTGGGTTTGTCTCCTGAAACGTATTTCTCCATTCATACTGATAATGAGGATGGCACCCAACTAGGTGGAAGCTCTTTACCACCTCCTTTTCCATCCTGCACCCTCAGAACAGCATTGACTCGATATGCAGATCTTTTAAATTCACCCAAAAAGGTATGTTTTGGAGTTGTTGCTTCATGCTTCTGGTTCACTGGGAGATCTATCTGTCAACTGAGGTGATTAATCTAGTCTTACTTATGCCTTTTCAGTCAGCATTGCTCGCATTAGCAGCACATGCTTCAAATCCTATAGAGGCTGACCGATTAAGATATCTTGCATCACCTGCTGGGAAGGTATTGATTTCTATCAGAAAATAAATTGGACACGTTTCTGCTTCTCTAACCTCACCCACTTACTTATTTGGTTAAAAACTAAAAATCGTGTAGGATGAATATTCTCAGTCTGTGGTTGGTAGCCAGAAAAGCCTGCTTGAAGTCATGGCTGAATTTCCTTCTGCCAAGCCTCCACTTGGTGTCTTCTTTGCAGCTGTTGCACCACGTTTACAGCCTCGATTCTACTCCATATCATCATCTCCAAGGTATTTACAATTTCCCTACCCTCTCCTTTAGCATTTTTTATTGTCTATTGAAACTTCTTTAACTGGTTTGGTATATAATATCCAAGAGAATTTTGTGTGATTAGAACCAAACAATTTGTTTATTTTCATTACATAAACGGAGGCTTGCAATGTGCAATGTATAGAAGTTCTTACCTGCTCCAGTTGGTTAGAAAACTAACATTTTTTAAATTTTCTATTGTTTTGGAAAAGCTTATTATTTCTTAAAGATAAGTTGCAAGGAAGGGAATATAAGTTAGGATTTTAGGCTGAGTTGATTAGAATAAAGATTTAATGGACGACTGTTTGGTGAAATGATTTTGGAAAGAAATCACTTCTCCTAAATTGTCATTTTGTCTAGGCATATTACTGCACGATGCATCTTTCCTATCATCCATGTATCTCTCTTTTCATCTTCCTTTCTCTCCTTTTCAAAGTTCTCTTTATGCTGAATCTTCGATATCTTTGTGCCTTTAACAGGATGGCTCCATCTAGAATTCATGTTACTTGTGCTCTTGTCTATGACAAAATGCCAACTGGACGTATTCATAAAGGAATTTGCTCTACTTGGATGAAGGTATGTAAAATGAAAGACATAATCCCGAGAAGCTAGTATTTTTTCAGAGAGTTATCCAATATTAGATGAATAGCAATTGTCCTGAATGTTTAGAGTTCTGAGCATCTTTAGATGTTTATTATGTAAATGATTCATGTTAAACTTCAGATGTTATGGATCATCATACAATAGCTCGGATATCTTTTTTGCTTGATATGTTAACAAGTGAAGGTTTCCACTGGGAAATTGGTGTAGGGTTTTTTTTCTTTTTTTTGTGGGGATACAAACACACAGTGATTTCTAACCTCATTTTGATTTGTATTTTATTCTGTTTCCTGATGACTGCTTTTAAGAGATTTAGTTCATCAATTTTCTCGTTGGTTTCAGATATTAATTAGTGATTGGCAGCTCATATCATAGATTGCCTCCTTCTGGCTATGAGAATCTAATAATTATTCTGCATTTTTCAGAATTCTGTGCCCATGGAGAAAATCCATGAGTGCAGTTGGGCTCCAATTTTTGTGAGGCAATCAAACTTCAAGCTTCCTTCTGATAGTAAAGTGCCTATTATCATGGTTGGTCCTGGAACTGGATTGGCTCCTTTCAGAGGTTTCTTACAGGTTTCAACTCGCACACTCCCCTTTGATTTTCTTCTACTTGAACTTTTGCGCTTCTAGAAAATAAACAAGTCGAAATATTTTATCTTCAGGAAAGATTAGCTTTGAAAGAATCTGGAGTAGAATTGGGGCCTTCCATATTGTTCTTTGGATGCAGAAACCGTGCAATGGTATGCCAAGTTCCTAGATTCAAGCTTGTACTTCGGCTATTGAAGAAATACATAGTTTATTTATAGATTTTCAAACTCATGCTTTTTATCTACTTTTGAAGGATTATATATACGAGGATGAGCTGAACAACTTTGTCGAGACTGGTGCTCTCTCCGAGTTGGTTATCGCCTTCTCGCGTGAAGGTCCAACGAAAGAATACGTGCAACATAAAATGACAGAGAAGGTGAAAAGACAATCTTAACAACCTATATAAGATTTCGGATTCATCATGGAGGAAGATATTAAAAAGCATTTTTTTTCTCTCTTTAAATATGCAGGCGTCAGACATCTGGAATTTGATATCACAAGGTGCTTACTTATATGTATGCGGTGATGCAAAGGGAATGGCTAGAGACGTCCACAGAACTCTCCACACCATCGTGCAAGAACAGGTAGTTTCACAAGTCATAACTCAACCAATAAAGTCATATATTTTATACGCAGTCTCGAACAATCAATTGTTAGGCCTATTCGCTAACTACGGATGTCGATTTCAGGGATCTCTTGACAGCTCGAAAGCTGAGAGCATGGTGAAGAATCTACAAACGAGCGGAAGGTATCTGCGTGATGTGTGGTGATAAAACTGCTCAACCCATGTGATCTCCAGAAAGGCATAATAATTAATAAGAAAAAAGTAAATGTGGTTGAATTAAATTATTATTGAGGTTCCTATGTCAATCACTTGGATTGAGACTTTTGATGGGTCTTCTTCTTAGCGAGGCAGAGGTTATGGAATTCTCTTCTTTTCTATCTCCCCATTTTGATCTACAGCTAAACATGAAGGATTCTTTTGTTCTTGTAATTTATAAAATACAGAATGTAGTTTTGTCATTAGTTAGAGCTCTCGTTTCTCTTAGTGTTATTGTATCAACCAACAATATGGTTCAAACTTCAAATTCATATATACTTTGCTGTAATATTAAACGGATCAAATAATTTCCAATTTTGTCTTCATACTTTG

Coding sequence (CDS)

ATGCAATCGGAATCCAGTTCTATGAAGGCTTCTCCATTTGACTTCATGTCGGCTATAATTAAGGGCAGGATGGATCCGTCTAATTCTTCATTTCAATCGACTGGCGAGGGTGCCTCAGTTATTTTCGAGAATCGCGAGCTGGTTGCGATCTTAACTACCTCGATCGCTGTCATGATTGGCTGCTTTGTTGTTCTTGTGTGGCGAAGATCCGGAAATCGAAAAGTTAAGACTATAGAGCTTCCTAAGCCGTTGCTTGGGAAGGAGCCAGAGCCAGAAGTTGACGACGGGAAGAAGAAGGTTACGATATTCTTTGGTACGCAGACTGGTACTGCTGAAGGCTTTGCAAAGGCTCTATCTGACGAGGCGAAAGCACGGTACGATAAGGCCAAGTTTAGAGTTGTTGATTTGGATGATTATGGGGCTGACGAAGATGAATACGAACAAAAATTGAAAAAGGAGTCTGTAGCTGTTTTCTTCTTGGCAACGTATGGCGATGGAGAGCCCACTGATAATGCCGCAAGATTCTATAAATGGTTCACCGAGGGTAAAGAGAGAGGGGAATGTCTTCAGAACCTCAATTATGCAGTCTTTGGCCTTGGCAACCGACAATATGAGCATTTTAATAAGATTGCAAAAGTGGTTGATGAGCTGCTTGAGACTCAGGGTGGTAAGCGCCTTGTAAAAGTTGGACTTGGAGATGACGATCAGTGCATAGAGGATGACTTCTCTGCTTGGCGAGAATCATTGTGGCCTGAGTTGGATCAATTGCTTCGGGATGAGGATGATGCAGCAACTGTGACCACACCTTACACAGCTGCCATATCAGAATACCGAGTGGTATTCCATGATCCTTCAGATGTAACTGATGACAAAAAGAACTGGATGAATGCAAATGGTCATGCTGTACATGACGCACAACATCCATTCAGATCTAATGTGGTTGTGAGAAAGGAGCTCCATACACCTGCGTCTGATCGTTCTTGTACTCATCTAGAGTTTGATATTTCTGAGTCTGCACTCAAATATGAAACAGGGGATCATGTTGGTGTTTACTGTGAAAATTTAACCGAGACTGTTGATGAGGCTCTAAATTTATTGGGTTTGTCTCCTGAAACGTATTTCTCCATTCATACTGATAATGAGGATGGCACCCAACTAGGTGGAAGCTCTTTACCACCTCCTTTTCCATCCTGCACCCTCAGAACAGCATTGACTCGATATGCAGATCTTTTAAATTCACCCAAAAAGTCAGCATTGCTCGCATTAGCAGCACATGCTTCAAATCCTATAGAGGCTGACCGATTAAGATATCTTGCATCACCTGCTGGGAAGGATGAATATTCTCAGTCTGTGGTTGGTAGCCAGAAAAGCCTGCTTGAAGTCATGGCTGAATTTCCTTCTGCCAAGCCTCCACTTGGTGTCTTCTTTGCAGCTGTTGCACCACGTTTACAGCCTCGATTCTACTCCATATCATCATCTCCAAGGATGGCTCCATCTAGAATTCATGTTACTTGTGCTCTTGTCTATGACAAAATGCCAACTGGACGTATTCATAAAGGAATTTGCTCTACTTGGATGAAGAATTCTGTGCCCATGGAGAAAATCCATGAGTGCAGTTGGGCTCCAATTTTTGTGAGGCAATCAAACTTCAAGCTTCCTTCTGATAGTAAAGTGCCTATTATCATGGTTGGTCCTGGAACTGGATTGGCTCCTTTCAGAGGTTTCTTACAGGAAAGATTAGCTTTGAAAGAATCTGGAGTAGAATTGGGGCCTTCCATATTGTTCTTTGGATGCAGAAACCGTGCAATGGATTATATATACGAGGATGAGCTGAACAACTTTGTCGAGACTGGTGCTCTCTCCGAGTTGGTTATCGCCTTCTCGCGTGAAGGTCCAACGAAAGAATACGTGCAACATAAAATGACAGAGAAGGCGTCAGACATCTGGAATTTGATATCACAAGGTGCTTACTTATATGTATGCGGTGATGCAAAGGGAATGGCTAGAGACGTCCACAGAACTCTCCACACCATCGTGCAAGAACAGGGATCTCTTGACAGCTCGAAAGCTGAGAGCATGGTGAAGAATCTACAAACGAGCGGAAGGTATCTGCGTGATGTGTGGTGA

Protein sequence

MQSESSSMKASPFDFMSAIIKGRMDPSNSSFQSTGEGASVIFENRELVAILTTSIAVMIGCFVVLVWRRSGNRKVKTIELPKPLLGKEPEPEVDDGKKKVTIFFGTQTGTAEGFAKALSDEAKARYDKAKFRVVDLDDYGADEDEYEQKLKKESVAVFFLATYGDGEPTDNAARFYKWFTEGKERGECLQNLNYAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQCIEDDFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDPSDVTDDKKNWMNANGHAVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDISESALKYETGDHVGVYCENLTETVDEALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNSPKKSALLALAAHASNPIEADRLRYLASPAGKDEYSQSVVGSQKSLLEVMAEFPSAKPPLGVFFAAVAPRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEKIHECSWAPIFVRQSNFKLPSDSKVPIIMVGPGTGLAPFRGFLQERLALKESGVELGPSILFFGCRNRAMDYIYEDELNNFVETGALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGAYLYVCGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVKNLQTSGRYLRDVW
Homology
BLAST of Cucsat.G18488.T1 vs. ExPASy Swiss-Prot
Match: A0A2U1LIM9 (NADPH--cytochrome P450 reductase 1 OS=Artemisia annua OX=35608 GN=CPR1 PE=1 SV=2)

HSP 1 Score: 1088.6 bits (2814), Expect = 0.0e+00
Identity = 530/709 (74.75%), Postives = 610/709 (86.04%), Query Frame = 0

Query: 1   MQSESSSMKASPFDFMSAIIKGRMDPSNSSFQSTGEGASVIFENRELVAILTTSIAVMIG 60
           MQS ++S+K SPFD M+A++ G++    S+   T    +V  ENREL+ ILTTS+AV+IG
Sbjct: 1   MQS-TTSVKLSPFDLMTALLNGKVSFDTSNTSDTNIPLAVFMENRELLMILTTSVAVLIG 60

Query: 61  CFVVLVWRRSGNRKVKTIELPKPLLGKE-PEPEVDDGKKKVTIFFGTQTGTAEGFAKALS 120
           C VVLVWRRS +   K  E P  ++ K+  E EVDDG+KKVT+FFGTQTGTAEGFAKAL 
Sbjct: 61  CVVVLVWRRSSSAAKKAAESPVIVVPKKVTEDEVDDGRKKVTVFFGTQTGTAEGFAKALV 120

Query: 121 DEAKARYDKAKFRVVDLDDYGADEDEYEQKLKKESVAVFFLATYGDGEPTDNAARFYKWF 180
           +EAKARY+KA F+V+DLDDY A++DEYE+KLKKES+A FFLATYGDGEPTDNAARFYKWF
Sbjct: 121 EEAKARYEKAVFKVIDLDDYAAEDDEYEEKLKKESLAFFFLATYGDGEPTDNAARFYKWF 180

Query: 181 TEGKERGECLQNLNYAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQCIE 240
           TEG+E+GE L+ L YAVFGLGNRQYEHFNKIAKVVDE L  QG KRLV VG+GDDDQCIE
Sbjct: 181 TEGEEKGEWLEKLQYAVFGLGNRQYEHFNKIAKVVDEKLVEQGAKRLVPVGMGDDDQCIE 240

Query: 241 DDFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDPSDVTDDKKNWMNANG 300
           DDF+AW+E +WPELDQLLRDEDD  +V TPYTAA++EYRVVFHD  +  D  +     NG
Sbjct: 241 DDFTAWKELVWPELDQLLRDEDD-TSVATPYTAAVAEYRVVFHDKPETYDQDQ---LTNG 300

Query: 301 HAVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDISESALKYETGDHVGVYCENLTETV 360
           HAVHDAQHP RSNV V+KELH+P SDRSCTHLEFDIS + L YETGDHVGVY ENL+E V
Sbjct: 301 HAVHDAQHPCRSNVAVKKELHSPLSDRSCTHLEFDISNTGLSYETGDHVGVYVENLSEVV 360

Query: 361 DEALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNSPKKSAL 420
           DEA  L+GL P TYFS+HTDNEDGT LGG+SLPPPFP CTLR AL  YAD+L+SPKKSAL
Sbjct: 361 DEAEKLIGLPPHTYFSVHTDNEDGTPLGGASLPPPFPPCTLRKALASYADVLSSPKKSAL 420

Query: 421 LALAAHASNPIEADRLRYLASPAGKDEYSQSVVGSQKSLLEVMAEFPSAKPPLGVFFAAV 480
           LALAAHA++  EADRL++LASPAGKDEY+Q +V S +SLLEVM  FPSAKPPLGVFFA+V
Sbjct: 421 LALAAHATDSTEADRLKFLASPAGKDEYAQWIVASHRSLLEVMEAFPSAKPPLGVFFASV 480

Query: 481 APRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEKIHECS 540
           APRLQPR+YSISSSP+ AP+RIHVTCALVY++ P+GR+HKG+CSTWMKN+VPM +  +CS
Sbjct: 481 APRLQPRYYSISSSPKFAPNRIHVTCALVYEQTPSGRVHKGVCSTWMKNAVPMTESQDCS 540

Query: 541 WAPIFVRQSNFKLPSDSKVPIIMVGPGTGLAPFRGFLQERLALKESGVELGPSILFFGCR 600
           WAPI+VR SNF+LPSD KVP+IM+GPGTGLAPFRGFLQERLA KE+G ELG +ILFFGCR
Sbjct: 541 WAPIYVRTSNFRLPSDPKVPVIMIGPGTGLAPFRGFLQERLAQKEAGTELGTAILFFGCR 600

Query: 601 NRAMDYIYEDELNNFVETGALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGAYLY 660
           NR +D+IYEDELNNFVETGALSELV AFSREG TKEYVQHKMT+KASDIWNL+S+GAYLY
Sbjct: 601 NRKVDFIYEDELNNFVETGALSELVTAFSREGATKEYVQHKMTQKASDIWNLLSEGAYLY 660

Query: 661 VCGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVKNLQTSGRYLRDVW 709
           VCGDAKGMA+DVHRTLHTIVQEQGSLDSSKAE  VKNLQ +GRYLRDVW
Sbjct: 661 VCGDAKGMAKDVHRTLHTIVQEQGSLDSSKAELYVKNLQMAGRYLRDVW 704

BLAST of Cucsat.G18488.T1 vs. ExPASy Swiss-Prot
Match: Q9SUM3 (NADPH--cytochrome P450 reductase 2 OS=Arabidopsis thaliana OX=3702 GN=ATR2 PE=1 SV=1)

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 534/712 (75.00%), Postives = 611/712 (85.81%), Query Frame = 0

Query: 3   SESSSMKASPFDFMSAIIKGR----MDPSN-SSFQS-TGEGASVIFENRELVAILTTSIA 62
           S SSS   S  D M+AIIKG      DP+N S+++S   E +S++ ENR+   I+TTSIA
Sbjct: 2   SSSSSSSTSMIDLMAAIIKGEPVIVSDPANASAYESVAAELSSMLIENRQFAMIVTTSIA 61

Query: 63  VMIGCFVVLVWRRSGNRKVKTIELPKPLLGKEPEPEVDDGKKKVTIFFGTQTGTAEGFAK 122
           V+IGC V+LVWRRSG+   K +E  KPL+ K  E E+DDG+KKVTIFFGTQTGTAEGFAK
Sbjct: 62  VLIGCIVMLVWRRSGSGNSKRVEPLKPLVIKPREEEIDDGRKKVTIFFGTQTGTAEGFAK 121

Query: 123 ALSDEAKARYDKAKFRVVDLDDYGADEDEYEQKLKKESVAVFFLATYGDGEPTDNAARFY 182
           AL +EAKARY+K +F++VDLDDY AD+DEYE+KLKKE VA FFLATYGDGEPTDNAARFY
Sbjct: 122 ALGEEAKARYEKTRFKIVDLDDYAADDDEYEEKLKKEDVAFFFLATYGDGEPTDNAARFY 181

Query: 183 KWFTEGKERGECLQNLNYAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQ 242
           KWFTEG +RGE L+NL Y VFGLGNRQYEHFNK+AKVVD++L  QG +RLV+VGLGDDDQ
Sbjct: 182 KWFTEGNDRGEWLKNLKYGVFGLGNRQYEHFNKVAKVVDDILVEQGAQRLVQVGLGDDDQ 241

Query: 243 CIEDDFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDPSDVTDDKKNWMN 302
           CIEDDF+AWRE+LWPELD +LR+E D A V TPYTAA+ EYRV  HD  D   +  N  N
Sbjct: 242 CIEDDFTAWREALWPELDTILREEGDTA-VATPYTAAVLEYRVSIHDSEDAKFNDINMAN 301

Query: 303 ANGHAVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDISESALKYETGDHVGVYCENLT 362
            NG+ V DAQHP+++NV V++ELHTP SDRSC HLEFDI+ S L YETGDHVGV C+NL+
Sbjct: 302 GNGYTVFDAQHPYKANVAVKRELHTPESDRSCIHLEFDIAGSGLTYETGDHVGVLCDNLS 361

Query: 363 ETVDEALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNSPKK 422
           ETVDEAL LL +SP+TYFS+H + EDGT +  SSLPPPFP C LRTALTRYA LL+SPKK
Sbjct: 362 ETVDEALRLLDMSPDTYFSLHAEKEDGTPI-SSSLPPPFPPCNLRTALTRYACLLSSPKK 421

Query: 423 SALLALAAHASNPIEADRLRYLASPAGKDEYSQSVVGSQKSLLEVMAEFPSAKPPLGVFF 482
           SAL+ALAAHAS+P EA+RL++LASPAGKDEYS+ VV SQ+SLLEVMAEFPSAKPPLGVFF
Sbjct: 422 SALVALAAHASDPTEAERLKHLASPAGKDEYSKWVVESQRSLLEVMAEFPSAKPPLGVFF 481

Query: 483 AAVAPRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEKIH 542
           A VAPRLQPRFYSISSSP++A +RIHVTCALVY+KMPTGRIHKG+CSTWMKN+VP EK  
Sbjct: 482 AGVAPRLQPRFYSISSSPKIAETRIHVTCALVYEKMPTGRIHKGVCSTWMKNAVPYEKSE 541

Query: 543 ECSWAPIFVRQSNFKLPSDSKVPIIMVGPGTGLAPFRGFLQERLALKESGVELGPSILFF 602
            CS APIFVRQSNFKLPSDSKVPIIM+GPGTGLAPFRGFLQERLAL ESGVELGPS+LFF
Sbjct: 542 NCSSAPIFVRQSNFKLPSDSKVPIIMIGPGTGLAPFRGFLQERLALVESGVELGPSVLFF 601

Query: 603 GCRNRAMDYIYEDELNNFVETGALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGA 662
           GCRNR MD+IYE+EL  FVE+GAL+EL +AFSREGPTKEYVQHKM +KASDIWN+ISQGA
Sbjct: 602 GCRNRRMDFIYEEELQRFVESGALAELSVAFSREGPTKEYVQHKMMDKASDIWNMISQGA 661

Query: 663 YLYVCGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVKNLQTSGRYLRDVW 709
           YLYVCGDAKGMARDVHR+LHTI QEQGS+DS+KAE  VKNLQTSGRYLRDVW
Sbjct: 662 YLYVCGDAKGMARDVHRSLHTIAQEQGSMDSTKAEGFVKNLQTSGRYLRDVW 711

BLAST of Cucsat.G18488.T1 vs. ExPASy Swiss-Prot
Match: Q05001 (NADPH--cytochrome P450 reductase OS=Catharanthus roseus OX=4058 GN=CPR PE=2 SV=1)

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 532/715 (74.41%), Postives = 612/715 (85.59%), Query Frame = 0

Query: 4   ESSSMKASPFDFMSAIIKG-RMDPSNSSFQSTGEGASV---IFENRELVAILTTSIAVMI 63
           +SSS K SPF+ MSAI+KG ++D SNSS        +V   + EN+ELV ILTTS+AV+I
Sbjct: 2   DSSSEKLSPFELMSAILKGAKLDGSNSSDSGVAVSPAVMAMLLENKELVMILTTSVAVLI 61

Query: 64  GCFVVLVWRRSGNRKVKTIELPKPLLGK---EPEPEVDDGKKKVTIFFGTQTGTAEGFAK 123
           GC VVL+WRRS     K +E PK ++ K   EPE E+D+GKKK TIFFGTQTGTAEGFAK
Sbjct: 62  GCVVVLIWRRSSGSGKKVVEPPKLIVPKSVVEPE-EIDEGKKKFTIFFGTQTGTAEGFAK 121

Query: 124 ALSDEAKARYDKAKFRVVDLDDYGADEDEYEQKLKKESVAVFFLATYGDGEPTDNAARFY 183
           AL++EAKARY+KA  +V+D+DDY AD++EYE+K +KE++A F LATYGDGEPTDNAARFY
Sbjct: 122 ALAEEAKARYEKAVIKVIDIDDYAADDEEYEEKFRKETLAFFILATYGDGEPTDNAARFY 181

Query: 184 KWFTEGKERGECLQNLNYAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQ 243
           KWF EG +RG+ L+NL Y VFGLGNRQYEHFNKIAKVVDE +  QGGKR+V + LGDDDQ
Sbjct: 182 KWFVEGNDRGDWLKNLQYGVFGLGNRQYEHFNKIAKVVDEKVAEQGGKRIVPLVLGDDDQ 241

Query: 244 CIEDDFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDPSDVTDDKKNWM- 303
           CIEDDF+AWRE++WPELD LLRDEDD  TV+T YTAAI EYRVVF D SD    + N   
Sbjct: 242 CIEDDFAAWRENVWPELDNLLRDEDD-TTVSTTYTAAIPEYRVVFPDKSDSLISEANGHA 301

Query: 304 --NANGHAVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDISESALKYETGDHVGVYCE 363
              ANG+ V+DAQHP RSNV VRKELHTPASDRSCTHL+FDI+ + L Y TGDHVGVYC+
Sbjct: 302 NGYANGNTVYDAQHPCRSNVAVRKELHTPASDRSCTHLDFDIAGTGLSYGTGDHVGVYCD 361

Query: 364 NLTETVDEALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNS 423
           NL+ETV+EA  LL L PETYFS+H D EDGT L GSSLPPPFP CTLRTALTRYADLLN+
Sbjct: 362 NLSETVEEAERLLNLPPETYFSLHADKEDGTPLAGSSLPPPFPPCTLRTALTRYADLLNT 421

Query: 424 PKKSALLALAAHASNPIEADRLRYLASPAGKDEYSQSVVGSQKSLLEVMAEFPSAKPPLG 483
           PKKSALLALAA+AS+P EADRL+YLASPAGKDEY+QS+V +Q+SLLEVMAEFPSAKPPLG
Sbjct: 422 PKKSALLALAAYASDPNEADRLKYLASPAGKDEYAQSLVANQRSLLEVMAEFPSAKPPLG 481

Query: 484 VFFAAVAPRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPME 543
           VFFAA+APRLQPRFYSISSSPRMAPSRIHVTCALVY+K P GRIHKG+CSTWMKN++P+E
Sbjct: 482 VFFAAIAPRLQPRFYSISSSPRMAPSRIHVTCALVYEKTPGGRIHKGVCSTWMKNAIPLE 541

Query: 544 KIHECSWAPIFVRQSNFKLPSDSKVPIIMVGPGTGLAPFRGFLQERLALKESGVELGPSI 603
           +  +CSWAPIFVRQSNFKLP+D KVP+IM+GPGTGLAPFRGFLQERLALKE G ELG ++
Sbjct: 542 ESRDCSWAPIFVRQSNFKLPADPKVPVIMIGPGTGLAPFRGFLQERLALKEEGAELGTAV 601

Query: 604 LFFGCRNRAMDYIYEDELNNFVETGALSELVIAFSREGPTKEYVQHKMTEKASDIWNLIS 663
            FFGCRNR MDYIYEDELN+F+E GALSEL++AFSREGPTK+YVQHKM EKASDIW +IS
Sbjct: 602 FFFGCRNRKMDYIYEDELNHFLEIGALSELLVAFSREGPTKQYVQHKMAEKASDIWRMIS 661

Query: 664 QGAYLYVCGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVKNLQTSGRYLRDVW 709
            GAY+YVCGDAKGMARDVHRTLHTI QEQGS+DS++AE  VKNLQ +GRYLRDVW
Sbjct: 662 DGAYVYVCGDAKGMARDVHRTLHTIAQEQGSMDSTQAEGFVKNLQMTGRYLRDVW 714

BLAST of Cucsat.G18488.T1 vs. ExPASy Swiss-Prot
Match: A0A2U1KZS6 (NADPH--cytochrome P450 reductase 2 OS=Artemisia annua OX=35608 GN=CPR2 PE=3 SV=1)

HSP 1 Score: 1043.1 bits (2696), Expect = 1.4e-303
Identity = 505/711 (71.03%), Postives = 600/711 (84.39%), Query Frame = 0

Query: 4   ESSSMKASPFDFMSAIIKGRM-DPSNSSFQSTGEGA-SVIFENRELVAILTTSIAVMIGC 63
           ++ S++ SPFD  S+++  ++ D  N S + T   A  ++ ENR+++ + TT++AV+IGC
Sbjct: 2   QTDSVQVSPFDLASSLLNVKLTDVLNMSEELTMSPAMKMLVENRDILTLFTTTVAVLIGC 61

Query: 64  FVVLVWRRSGNRKVKT--IELPKPLLGKE--PEPEVDDGKKKVTIFFGTQTGTAEGFAKA 123
            VVLVWRRS  +K  T  +E  K ++ K+     EVDDGKK+VTI +GTQTGTAEGFAKA
Sbjct: 62  VVVLVWRRSFTKKSVTNEVETMKIVVPKKEIKHEEVDDGKKRVTILYGTQTGTAEGFAKA 121

Query: 124 LSDEAKARYDKAKFRVVDLDDYGADEDEYEQKLKKESVAVFFLATYGDGEPTDNAARFYK 183
             +EAK RY+KA F+ +DLDDY AD++EYE+K KKES+A FFLATYGDGEPTDNAARFYK
Sbjct: 122 FLEEAKVRYEKALFKAIDLDDYAADDEEYEEKFKKESLAFFFLATYGDGEPTDNAARFYK 181

Query: 184 WFTEGKERGECLQNLNYAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQC 243
           WFTEG ++GE L+ L Y VFGLGNRQYEHFNKIA VVD+ L  QG KRLV VGLGDDDQC
Sbjct: 182 WFTEGDDKGEWLKKLQYGVFGLGNRQYEHFNKIAVVVDDKLAEQGAKRLVSVGLGDDDQC 241

Query: 244 IEDDFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDPSDVTDDKKNWMNA 303
           +EDDF+AW+E +WP+LDQLLRDEDD  +V TPYTAA+ EYRVV+HD  D     ++  + 
Sbjct: 242 MEDDFTAWKELVWPQLDQLLRDEDD-MSVATPYTAAVLEYRVVYHDKPD--SSAEDHSHT 301

Query: 304 NGHAVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDISESALKYETGDHVGVYCENLTE 363
           NGHAV DAQHP RSNV  +KELHTP SDRSCTHLEFDI+ + L YETGDHVGVYCENL+E
Sbjct: 302 NGHAVPDAQHPIRSNVAFKKELHTPESDRSCTHLEFDIANTGLSYETGDHVGVYCENLSE 361

Query: 364 TVDEALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNSPKKS 423
            VDEA+ L+GL  +TYFS+H DNEDGT +GG+SLPPPFP CTLR AL RYAD+L+SPKKS
Sbjct: 362 VVDEAVRLVGLPADTYFSVHADNEDGTPIGGASLPPPFPPCTLRDALARYADVLSSPKKS 421

Query: 424 ALLALAAHASNPIEADRLRYLASPAGKDEYSQSVVGSQKSLLEVMAEFPSAKPPLGVFFA 483
           ALL LAAHAS+P EA+RL++LASPAGKDEY+Q +V SQ+SLLEVM  FPSAKPPLGVFFA
Sbjct: 422 ALLGLAAHASDPAEAERLKFLASPAGKDEYAQWIVASQRSLLEVMEAFPSAKPPLGVFFA 481

Query: 484 AVAPRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEKIHE 543
           A++PRLQPR+YSISSSP+M  ++IHVTCALVY+K P GRIHKG+CSTWMK++VPM +   
Sbjct: 482 AISPRLQPRYYSISSSPKMVENKIHVTCALVYEKTPVGRIHKGVCSTWMKDAVPMTESQV 541

Query: 544 CSWAPIFVRQSNFKLPSDSKVPIIMVGPGTGLAPFRGFLQERLALKESGVELGPSILFFG 603
            SWAPIFVR SNF+LPSD KVP+IM+GPGTGLAPFRGFLQERL+LKE+G ELG S+LFFG
Sbjct: 542 YSWAPIFVRTSNFRLPSDPKVPVIMIGPGTGLAPFRGFLQERLSLKEAGTELGSSVLFFG 601

Query: 604 CRNRAMDYIYEDELNNFVETGALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGAY 663
           CRNR +D+IYEDELNNFV+TGALSELV+AFSREGPTKEYVQHK+T+KASDIWNL+++GAY
Sbjct: 602 CRNRKVDFIYEDELNNFVKTGALSELVVAFSREGPTKEYVQHKLTQKASDIWNLLTEGAY 661

Query: 664 LYVCGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVKNLQTSGRYLRDVW 709
           LYVCGDAKGMA+DVHRTLHTIVQEQGSLDSSKAE  VKNLQ SGRYLRDVW
Sbjct: 662 LYVCGDAKGMAKDVHRTLHTIVQEQGSLDSSKAELYVKNLQMSGRYLRDVW 709

BLAST of Cucsat.G18488.T1 vs. ExPASy Swiss-Prot
Match: Q653S9 (NADPH--cytochrome P450 reductase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CPR3 PE=1 SV=1)

HSP 1 Score: 1028.9 bits (2659), Expect = 2.8e-299
Identity = 500/710 (70.42%), Postives = 588/710 (82.82%), Query Frame = 0

Query: 7   SMKASPFDFMSAIIKGRMDPSNSSFQSTGEGASVIFENRELVAILTTSIAVMIGCFVVLV 66
           +++ S  D ++A++ GR  P    +  +      + ENR L+ +LTTS+AV++GC V L+
Sbjct: 11  ALRPSALDLVAALLTGRGRPEEEGWPPS------LAENRHLIVLLTTSLAVLVGCGVALL 70

Query: 67  WRRSG-NRKVKTIELPKPLL------GKEPEPEVDDGKKKVTIFFGTQTGTAEGFAKALS 126
            RRS  +      + P+P         +E EP+ DDG+++V +FFGTQTGTAEGFAKAL+
Sbjct: 71  VRRSSISAPAVRAQEPQPRAPAPAKRKQEAEPDPDDGRQRVAVFFGTQTGTAEGFAKALA 130

Query: 127 DEAKARYDKAKFRVVDLDDYGADEDEYEQKLKKESVAVFFLATYGDGEPTDNAARFYKWF 186
           +EAK+RYDKA F+V+DLD+Y AD++EYEQKLKKE +A+FF+ATYGDGEPTDNAARFYKWF
Sbjct: 131 EEAKSRYDKAVFKVLDLDEYAADDEEYEQKLKKEIIALFFVATYGDGEPTDNAARFYKWF 190

Query: 187 TEGKERGECLQNLNYAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQCIE 246
            EG ERGE L NL + VFGLGNRQYEHFNK+ KVVD+LL  QGGKR+V +GLGDDDQCIE
Sbjct: 191 GEGNERGEWLSNLRFGVFGLGNRQYEHFNKVGKVVDQLLAEQGGKRIVPLGLGDDDQCIE 250

Query: 247 DDFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDPSDVTDDKKNWMNANG 306
           DDF+AW+E LWPELD+LLR EDD +   TPYTAAI EYRVV   P +     K++  +NG
Sbjct: 251 DDFNAWKELLWPELDKLLRVEDDKSAAPTPYTAAIPEYRVVLVKPEEAMHINKSFSLSNG 310

Query: 307 HAVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDISESALKYETGDHVGVYCENLTETV 366
           HAV+D QHP R+NV VR+ELHTPAS RSC HLEFDIS + L YETGDHVGVY EN TETV
Sbjct: 311 HAVYDIQHPCRANVAVRRELHTPASYRSCIHLEFDISGTGLTYETGDHVGVYAENCTETV 370

Query: 367 DEALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPS-CTLRTALTRYADLLNSPKKSA 426
           +E  NLLG SP+T FSIH D EDGT L G SLPPPFPS CT+ TAL RYADLL+ PKKSA
Sbjct: 371 EEVENLLGYSPDTLFSIHADQEDGTPLFGGSLPPPFPSPCTVGTALARYADLLSFPKKSA 430

Query: 427 LLALAAHASNPIEADRLRYLASPAGKDEYSQSVVGSQKSLLEVMAEFPSAKPPLGVFFAA 486
           L+ALA+HAS+P +A+RLR+LASPAGK EYSQ +V SQ+SLLEVM EFPSAKPPLGVFFAA
Sbjct: 431 LIALASHASDPKDAERLRHLASPAGKKEYSQWIVSSQRSLLEVMTEFPSAKPPLGVFFAA 490

Query: 487 VAPRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEKIHEC 546
           +APRLQPR+YSISSSPRM P+RIHVTCALVY + PTGRIHKG+CSTWMKNS+P+E+  EC
Sbjct: 491 IAPRLQPRYYSISSSPRMTPTRIHVTCALVYGQTPTGRIHKGVCSTWMKNSIPLEESQEC 550

Query: 547 SWAPIFVRQSNFKLPSDSKVPIIMVGPGTGLAPFRGFLQERLALKESGVELGPSILFFGC 606
           SWAPIFVRQSNFKLP+D  VPIIM+GPGTGLAPFRGFLQERLALKE+GVELG ++LFFGC
Sbjct: 551 SWAPIFVRQSNFKLPTDPTVPIIMIGPGTGLAPFRGFLQERLALKETGVELGHAVLFFGC 610

Query: 607 RNRAMDYIYEDELNNFVETGALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGAYL 666
           RNR MD+IYEDELNNFVETGALSEL++AFSREGP+KEYVQHKM EKA +IW++ISQG Y+
Sbjct: 611 RNRKMDFIYEDELNNFVETGALSELIVAFSREGPSKEYVQHKMAEKAPEIWSIISQGGYI 670

Query: 667 YVCGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVKNLQTSGRYLRDVW 709
           YVCGDAKGMARDVHRTLHTIVQEQGSLD+S  ES VK+LQ  GRYLRDVW
Sbjct: 671 YVCGDAKGMARDVHRTLHTIVQEQGSLDNSNTESYVKSLQMEGRYLRDVW 714

BLAST of Cucsat.G18488.T1 vs. NCBI nr
Match: XP_004146317.1 (NADPH--cytochrome P450 reductase 2 [Cucumis sativus] >AIT72028.1 cytochrome P450 reductase [Cucumis sativus] >KGN65457.1 hypothetical protein Csa_019925 [Cucumis sativus])

HSP 1 Score: 1427 bits (3693), Expect = 0.0
Identity = 708/708 (100.00%), Postives = 708/708 (100.00%), Query Frame = 0

Query: 1   MQSESSSMKASPFDFMSAIIKGRMDPSNSSFQSTGEGASVIFENRELVAILTTSIAVMIG 60
           MQSESSSMKASPFDFMSAIIKGRMDPSNSSFQSTGEGASVIFENRELVAILTTSIAVMIG
Sbjct: 1   MQSESSSMKASPFDFMSAIIKGRMDPSNSSFQSTGEGASVIFENRELVAILTTSIAVMIG 60

Query: 61  CFVVLVWRRSGNRKVKTIELPKPLLGKEPEPEVDDGKKKVTIFFGTQTGTAEGFAKALSD 120
           CFVVLVWRRSGNRKVKTIELPKPLLGKEPEPEVDDGKKKVTIFFGTQTGTAEGFAKALSD
Sbjct: 61  CFVVLVWRRSGNRKVKTIELPKPLLGKEPEPEVDDGKKKVTIFFGTQTGTAEGFAKALSD 120

Query: 121 EAKARYDKAKFRVVDLDDYGADEDEYEQKLKKESVAVFFLATYGDGEPTDNAARFYKWFT 180
           EAKARYDKAKFRVVDLDDYGADEDEYEQKLKKESVAVFFLATYGDGEPTDNAARFYKWFT
Sbjct: 121 EAKARYDKAKFRVVDLDDYGADEDEYEQKLKKESVAVFFLATYGDGEPTDNAARFYKWFT 180

Query: 181 EGKERGECLQNLNYAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQCIED 240
           EGKERGECLQNLNYAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQCIED
Sbjct: 181 EGKERGECLQNLNYAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQCIED 240

Query: 241 DFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDPSDVTDDKKNWMNANGH 300
           DFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDPSDVTDDKKNWMNANGH
Sbjct: 241 DFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDPSDVTDDKKNWMNANGH 300

Query: 301 AVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDISESALKYETGDHVGVYCENLTETVD 360
           AVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDISESALKYETGDHVGVYCENLTETVD
Sbjct: 301 AVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDISESALKYETGDHVGVYCENLTETVD 360

Query: 361 EALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNSPKKSALL 420
           EALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNSPKKSALL
Sbjct: 361 EALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNSPKKSALL 420

Query: 421 ALAAHASNPIEADRLRYLASPAGKDEYSQSVVGSQKSLLEVMAEFPSAKPPLGVFFAAVA 480
           ALAAHASNPIEADRLRYLASPAGKDEYSQSVVGSQKSLLEVMAEFPSAKPPLGVFFAAVA
Sbjct: 421 ALAAHASNPIEADRLRYLASPAGKDEYSQSVVGSQKSLLEVMAEFPSAKPPLGVFFAAVA 480

Query: 481 PRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEKIHECSW 540
           PRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEKIHECSW
Sbjct: 481 PRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEKIHECSW 540

Query: 541 APIFVRQSNFKLPSDSKVPIIMVGPGTGLAPFRGFLQERLALKESGVELGPSILFFGCRN 600
           APIFVRQSNFKLPSDSKVPIIMVGPGTGLAPFRGFLQERLALKESGVELGPSILFFGCRN
Sbjct: 541 APIFVRQSNFKLPSDSKVPIIMVGPGTGLAPFRGFLQERLALKESGVELGPSILFFGCRN 600

Query: 601 RAMDYIYEDELNNFVETGALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGAYLYV 660
           RAMDYIYEDELNNFVETGALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGAYLYV
Sbjct: 601 RAMDYIYEDELNNFVETGALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGAYLYV 660

Query: 661 CGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVKNLQTSGRYLRDVW 708
           CGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVKNLQTSGRYLRDVW
Sbjct: 661 CGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVKNLQTSGRYLRDVW 708

BLAST of Cucsat.G18488.T1 vs. NCBI nr
Match: XP_008453604.1 (PREDICTED: NADPH--cytochrome P450 reductase 2 [Cucumis melo])

HSP 1 Score: 1396 bits (3614), Expect = 0.0
Identity = 692/708 (97.74%), Postives = 699/708 (98.73%), Query Frame = 0

Query: 1   MQSESSSMKASPFDFMSAIIKGRMDPSNSSFQSTGEGASVIFENRELVAILTTSIAVMIG 60
           MQSESSSMKASP DFMSAIIKGRMDPSNSSFQSTGEGASVIFENRELVAILTTSIAVMIG
Sbjct: 1   MQSESSSMKASPLDFMSAIIKGRMDPSNSSFQSTGEGASVIFENRELVAILTTSIAVMIG 60

Query: 61  CFVVLVWRRSGNRKVKTIELPKPLLGKEPEPEVDDGKKKVTIFFGTQTGTAEGFAKALSD 120
           CFVVLVWRRSGNRKVKTIELPKPLL KEPEPEVDDGKKKVTIFFGTQTGTAEGFAKALSD
Sbjct: 61  CFVVLVWRRSGNRKVKTIELPKPLLAKEPEPEVDDGKKKVTIFFGTQTGTAEGFAKALSD 120

Query: 121 EAKARYDKAKFRVVDLDDYGADEDEYEQKLKKESVAVFFLATYGDGEPTDNAARFYKWFT 180
           EAKARYDKAKFRVVDLDDYG D+DEYEQ+LKKESVAVFFLATYGDGEPTDNAARFYKWFT
Sbjct: 121 EAKARYDKAKFRVVDLDDYGGDDDEYEQQLKKESVAVFFLATYGDGEPTDNAARFYKWFT 180

Query: 181 EGKERGECLQNLNYAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQCIED 240
           EGKERGE LQNL+YAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQCIED
Sbjct: 181 EGKERGEWLQNLSYAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQCIED 240

Query: 241 DFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDPSDVTDDKKNWMNANGH 300
           DFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDP+DVTDD KNWMNANGH
Sbjct: 241 DFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDPADVTDDNKNWMNANGH 300

Query: 301 AVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDISESALKYETGDHVGVYCENLTETVD 360
           AVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDIS SALKYETGDHVGVYCENLTETVD
Sbjct: 301 AVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDISGSALKYETGDHVGVYCENLTETVD 360

Query: 361 EALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNSPKKSALL 420
           EALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNSPKKSALL
Sbjct: 361 EALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNSPKKSALL 420

Query: 421 ALAAHASNPIEADRLRYLASPAGKDEYSQSVVGSQKSLLEVMAEFPSAKPPLGVFFAAVA 480
           ALAAHASNPIEADRLRYLASP+GKDEYSQSVVG QKSLLEVMAEFPSAKPPLGVFFAAVA
Sbjct: 421 ALAAHASNPIEADRLRYLASPSGKDEYSQSVVGCQKSLLEVMAEFPSAKPPLGVFFAAVA 480

Query: 481 PRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEKIHECSW 540
           PRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEK HECSW
Sbjct: 481 PRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEKSHECSW 540

Query: 541 APIFVRQSNFKLPSDSKVPIIMVGPGTGLAPFRGFLQERLALKESGVELGPSILFFGCRN 600
           APIFVRQSNFKLPSDSKVPIIM+GPGTGLAPFRGFLQERLALKESGVELGPSILFFGCRN
Sbjct: 541 APIFVRQSNFKLPSDSKVPIIMIGPGTGLAPFRGFLQERLALKESGVELGPSILFFGCRN 600

Query: 601 RAMDYIYEDELNNFVETGALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGAYLYV 660
           RAMDYIYEDELNNFVE GALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGAYLYV
Sbjct: 601 RAMDYIYEDELNNFVEAGALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGAYLYV 660

Query: 661 CGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVKNLQTSGRYLRDVW 708
           CGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVK+LQTSGRYLRDVW
Sbjct: 661 CGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVKSLQTSGRYLRDVW 708

BLAST of Cucsat.G18488.T1 vs. NCBI nr
Match: TYK11826.1 (NADPH--cytochrome P450 reductase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1395 bits (3611), Expect = 0.0
Identity = 692/708 (97.74%), Postives = 698/708 (98.59%), Query Frame = 0

Query: 1   MQSESSSMKASPFDFMSAIIKGRMDPSNSSFQSTGEGASVIFENRELVAILTTSIAVMIG 60
           MQSESSSMKASP DFMSAIIKGRMDPSNSSFQSTGEGASVIFENRELVAILTTSIAVMIG
Sbjct: 1   MQSESSSMKASPLDFMSAIIKGRMDPSNSSFQSTGEGASVIFENRELVAILTTSIAVMIG 60

Query: 61  CFVVLVWRRSGNRKVKTIELPKPLLGKEPEPEVDDGKKKVTIFFGTQTGTAEGFAKALSD 120
           CFVVLVWRRSGNRKVKTIELPKPLL KEPEPEVDDGKKKVTIFFGTQTGTAEGFAKALSD
Sbjct: 61  CFVVLVWRRSGNRKVKTIELPKPLLAKEPEPEVDDGKKKVTIFFGTQTGTAEGFAKALSD 120

Query: 121 EAKARYDKAKFRVVDLDDYGADEDEYEQKLKKESVAVFFLATYGDGEPTDNAARFYKWFT 180
           EAKARYDKAKFRVVDLDDYG D+DEYEQ+LKKESVAVFFLATYGDGEPTDNAARFYKWFT
Sbjct: 121 EAKARYDKAKFRVVDLDDYGGDDDEYEQQLKKESVAVFFLATYGDGEPTDNAARFYKWFT 180

Query: 181 EGKERGECLQNLNYAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQCIED 240
           EGKERGE LQNL+YAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQCIED
Sbjct: 181 EGKERGEWLQNLSYAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQCIED 240

Query: 241 DFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDPSDVTDDKKNWMNANGH 300
           DFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDP+DVTDD KNWMNANGH
Sbjct: 241 DFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDPADVTDDNKNWMNANGH 300

Query: 301 AVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDISESALKYETGDHVGVYCENLTETVD 360
           AVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDIS SALKYETGDHVGVYCENLTETVD
Sbjct: 301 AVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDISGSALKYETGDHVGVYCENLTETVD 360

Query: 361 EALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNSPKKSALL 420
           EALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNSPKKSALL
Sbjct: 361 EALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNSPKKSALL 420

Query: 421 ALAAHASNPIEADRLRYLASPAGKDEYSQSVVGSQKSLLEVMAEFPSAKPPLGVFFAAVA 480
           ALAAHASNPIEADRLRYLASPAGKDEYSQSVVG QKSLLEVMAEFPSAKPPLGVFFAAVA
Sbjct: 421 ALAAHASNPIEADRLRYLASPAGKDEYSQSVVGCQKSLLEVMAEFPSAKPPLGVFFAAVA 480

Query: 481 PRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEKIHECSW 540
           PRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEK HECSW
Sbjct: 481 PRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEKSHECSW 540

Query: 541 APIFVRQSNFKLPSDSKVPIIMVGPGTGLAPFRGFLQERLALKESGVELGPSILFFGCRN 600
           APIFVRQSNFKLPSDSKVPIIM+GPGTGLAPFRGFLQERLALKESGVELGPSILFFGCRN
Sbjct: 541 APIFVRQSNFKLPSDSKVPIIMIGPGTGLAPFRGFLQERLALKESGVELGPSILFFGCRN 600

Query: 601 RAMDYIYEDELNNFVETGALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGAYLYV 660
           RAMDYIYEDELNNFVE GALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGAYLYV
Sbjct: 601 RAMDYIYEDELNNFVEAGALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGAYLYV 660

Query: 661 CGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVKNLQTSGRYLRDVW 708
           CGDAKGMARDVHRTLHTIVQEQGSLDS KAESMVK+LQTSGRYLRDVW
Sbjct: 661 CGDAKGMARDVHRTLHTIVQEQGSLDSLKAESMVKSLQTSGRYLRDVW 708

BLAST of Cucsat.G18488.T1 vs. NCBI nr
Match: XP_038879587.1 (NADPH--cytochrome P450 reductase 1-like [Benincasa hispida])

HSP 1 Score: 1375 bits (3560), Expect = 0.0
Identity = 678/708 (95.76%), Postives = 694/708 (98.02%), Query Frame = 0

Query: 1   MQSESSSMKASPFDFMSAIIKGRMDPSNSSFQSTGEGASVIFENRELVAILTTSIAVMIG 60
           MQS+SSS+KASPF+FMSAIIKGRMDPSNSSFQSTGEGA VIFENRELVAILTTSIAVMIG
Sbjct: 1   MQSQSSSVKASPFEFMSAIIKGRMDPSNSSFQSTGEGALVIFENRELVAILTTSIAVMIG 60

Query: 61  CFVVLVWRRSGNRKVKTIELPKPLLGKEPEPEVDDGKKKVTIFFGTQTGTAEGFAKALSD 120
           CFVVLVWRRSGNR+VKT+ELPKPLL KEPEPEV+DGKKKVTIFFGTQTGTAEGFAKALSD
Sbjct: 61  CFVVLVWRRSGNRRVKTVELPKPLLAKEPEPEVEDGKKKVTIFFGTQTGTAEGFAKALSD 120

Query: 121 EAKARYDKAKFRVVDLDDYGADEDEYEQKLKKESVAVFFLATYGDGEPTDNAARFYKWFT 180
           EAKARYDKA FRVVDLDDYGAD+D+YEQKLKKESVA+FFLATYGDGEPTDN ARFYKWFT
Sbjct: 121 EAKARYDKATFRVVDLDDYGADDDDYEQKLKKESVALFFLATYGDGEPTDNTARFYKWFT 180

Query: 181 EGKERGECLQNLNYAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQCIED 240
           EGKERGE LQNL+YAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQCIED
Sbjct: 181 EGKERGEWLQNLHYAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQCIED 240

Query: 241 DFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDPSDVTDDKKNWMNANGH 300
           DF+AWRESLWPELDQLLRDEDDA TVTTPYTAAISEYRVVFHDP+DVTDD KNWMNANGH
Sbjct: 241 DFAAWRESLWPELDQLLRDEDDATTVTTPYTAAISEYRVVFHDPADVTDDNKNWMNANGH 300

Query: 301 AVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDISESALKYETGDHVGVYCENLTETVD 360
           AVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDIS SALKYETGDHVGVYCENL ETVD
Sbjct: 301 AVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDISGSALKYETGDHVGVYCENLAETVD 360

Query: 361 EALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNSPKKSALL 420
           EALNLLGLSPETYFSIHTDNEDGT LGGSSLPPPFPSCTLRTALTRYADLLNSPKKSALL
Sbjct: 361 EALNLLGLSPETYFSIHTDNEDGTPLGGSSLPPPFPSCTLRTALTRYADLLNSPKKSALL 420

Query: 421 ALAAHASNPIEADRLRYLASPAGKDEYSQSVVGSQKSLLEVMAEFPSAKPPLGVFFAAVA 480
           ALAAHASNP+EADRLRYLASPAGKDEYSQSVVGSQKSLLEVMAEFPSAKPPLGVFFAAVA
Sbjct: 421 ALAAHASNPVEADRLRYLASPAGKDEYSQSVVGSQKSLLEVMAEFPSAKPPLGVFFAAVA 480

Query: 481 PRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEKIHECSW 540
           PRLQPRFYSISSSPRMA SRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEK H+CSW
Sbjct: 481 PRLQPRFYSISSSPRMASSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEKSHDCSW 540

Query: 541 APIFVRQSNFKLPSDSKVPIIMVGPGTGLAPFRGFLQERLALKESGVELGPSILFFGCRN 600
           APIFVRQSNFKLP+DSKVPIIMVGPGTGLAPFRGFLQERLALKESGVELGPSILFFGCRN
Sbjct: 541 APIFVRQSNFKLPADSKVPIIMVGPGTGLAPFRGFLQERLALKESGVELGPSILFFGCRN 600

Query: 601 RAMDYIYEDELNNFVETGALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGAYLYV 660
           R MDYIYEDELNN+VETGALSELVIAFSREGPTKEYVQHKM EKASDIWNLISQGAYLYV
Sbjct: 601 RRMDYIYEDELNNYVETGALSELVIAFSREGPTKEYVQHKMAEKASDIWNLISQGAYLYV 660

Query: 661 CGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVKNLQTSGRYLRDVW 708
           CGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVKNLQTSGRYLRDVW
Sbjct: 661 CGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVKNLQTSGRYLRDVW 708

BLAST of Cucsat.G18488.T1 vs. NCBI nr
Match: KAA0058274.1 (NADPH--cytochrome P450 reductase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1375 bits (3558), Expect = 0.0
Identity = 681/708 (96.19%), Postives = 691/708 (97.60%), Query Frame = 0

Query: 1   MQSESSSMKASPFDFMSAIIKGRMDPSNSSFQSTGEGASVIFENRELVAILTTSIAVMIG 60
           MQSESSSMKASP DFMSAIIKGRMDPSNSSFQSTGEGASVIFENRELVAILTTSIAVMIG
Sbjct: 1   MQSESSSMKASPLDFMSAIIKGRMDPSNSSFQSTGEGASVIFENRELVAILTTSIAVMIG 60

Query: 61  CFVVLVWRRSGNRKVKTIELPKPLLGKEPEPEVDDGKKKVTIFFGTQTGTAEGFAKALSD 120
           CFVVLVWRRSGNRKVKTIELPKPLL KEPEPEVDDGKKKVTIFFGTQTGTAEGFAKALSD
Sbjct: 61  CFVVLVWRRSGNRKVKTIELPKPLLAKEPEPEVDDGKKKVTIFFGTQTGTAEGFAKALSD 120

Query: 121 EAKARYDKAKFRVVDLDDYGADEDEYEQKLKKESVAVFFLATYGDGEPTDNAARFYKWFT 180
           EAKARYDKAKFRVVDLDDYG D+DEYEQ+LKKESVAVFFLATYGDGEPTDNAARFYKWFT
Sbjct: 121 EAKARYDKAKFRVVDLDDYGGDDDEYEQQLKKESVAVFFLATYGDGEPTDNAARFYKWFT 180

Query: 181 EGKERGECLQNLNYAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQCIED 240
           EGKERGE LQNL+YAVFGLGNRQYEHFNK++ V  + L    GKRLVKVGLGDDDQCIED
Sbjct: 181 EGKERGEWLQNLSYAVFGLGNRQYEHFNKVSMVPSKFLGVNCGKRLVKVGLGDDDQCIED 240

Query: 241 DFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDPSDVTDDKKNWMNANGH 300
           DFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDP+DVTDD KNWMNANGH
Sbjct: 241 DFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDPADVTDDNKNWMNANGH 300

Query: 301 AVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDISESALKYETGDHVGVYCENLTETVD 360
           AVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDIS SALKYETGDHVGVYCENLTETVD
Sbjct: 301 AVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDISGSALKYETGDHVGVYCENLTETVD 360

Query: 361 EALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNSPKKSALL 420
           EALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNSPKKSALL
Sbjct: 361 EALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNSPKKSALL 420

Query: 421 ALAAHASNPIEADRLRYLASPAGKDEYSQSVVGSQKSLLEVMAEFPSAKPPLGVFFAAVA 480
           ALAAHASNPIEADRLRYLASP+GKDEYSQSVVG QKSLLEVMAEFPSAKPPLGVFFAAVA
Sbjct: 421 ALAAHASNPIEADRLRYLASPSGKDEYSQSVVGCQKSLLEVMAEFPSAKPPLGVFFAAVA 480

Query: 481 PRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEKIHECSW 540
           PRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEK HECSW
Sbjct: 481 PRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEKSHECSW 540

Query: 541 APIFVRQSNFKLPSDSKVPIIMVGPGTGLAPFRGFLQERLALKESGVELGPSILFFGCRN 600
           APIFVRQSNFKLPSDSKVPIIM+GPGTGLAPFRGFLQERLALKESGVELGPSILFFGCRN
Sbjct: 541 APIFVRQSNFKLPSDSKVPIIMIGPGTGLAPFRGFLQERLALKESGVELGPSILFFGCRN 600

Query: 601 RAMDYIYEDELNNFVETGALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGAYLYV 660
           RAMDYIYEDELNNFVE GALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGAYLYV
Sbjct: 601 RAMDYIYEDELNNFVEAGALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGAYLYV 660

Query: 661 CGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVKNLQTSGRYLRDVW 708
           CGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVK+LQTSGRYLRDVW
Sbjct: 661 CGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVKSLQTSGRYLRDVW 708

BLAST of Cucsat.G18488.T1 vs. ExPASy TrEMBL
Match: A0A0A0LXA1 (NADPH--cytochrome P450 reductase OS=Cucumis sativus OX=3659 GN=Csa1G423150 PE=2 SV=1)

HSP 1 Score: 1427 bits (3693), Expect = 0.0
Identity = 708/708 (100.00%), Postives = 708/708 (100.00%), Query Frame = 0

Query: 1   MQSESSSMKASPFDFMSAIIKGRMDPSNSSFQSTGEGASVIFENRELVAILTTSIAVMIG 60
           MQSESSSMKASPFDFMSAIIKGRMDPSNSSFQSTGEGASVIFENRELVAILTTSIAVMIG
Sbjct: 1   MQSESSSMKASPFDFMSAIIKGRMDPSNSSFQSTGEGASVIFENRELVAILTTSIAVMIG 60

Query: 61  CFVVLVWRRSGNRKVKTIELPKPLLGKEPEPEVDDGKKKVTIFFGTQTGTAEGFAKALSD 120
           CFVVLVWRRSGNRKVKTIELPKPLLGKEPEPEVDDGKKKVTIFFGTQTGTAEGFAKALSD
Sbjct: 61  CFVVLVWRRSGNRKVKTIELPKPLLGKEPEPEVDDGKKKVTIFFGTQTGTAEGFAKALSD 120

Query: 121 EAKARYDKAKFRVVDLDDYGADEDEYEQKLKKESVAVFFLATYGDGEPTDNAARFYKWFT 180
           EAKARYDKAKFRVVDLDDYGADEDEYEQKLKKESVAVFFLATYGDGEPTDNAARFYKWFT
Sbjct: 121 EAKARYDKAKFRVVDLDDYGADEDEYEQKLKKESVAVFFLATYGDGEPTDNAARFYKWFT 180

Query: 181 EGKERGECLQNLNYAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQCIED 240
           EGKERGECLQNLNYAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQCIED
Sbjct: 181 EGKERGECLQNLNYAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQCIED 240

Query: 241 DFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDPSDVTDDKKNWMNANGH 300
           DFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDPSDVTDDKKNWMNANGH
Sbjct: 241 DFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDPSDVTDDKKNWMNANGH 300

Query: 301 AVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDISESALKYETGDHVGVYCENLTETVD 360
           AVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDISESALKYETGDHVGVYCENLTETVD
Sbjct: 301 AVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDISESALKYETGDHVGVYCENLTETVD 360

Query: 361 EALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNSPKKSALL 420
           EALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNSPKKSALL
Sbjct: 361 EALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNSPKKSALL 420

Query: 421 ALAAHASNPIEADRLRYLASPAGKDEYSQSVVGSQKSLLEVMAEFPSAKPPLGVFFAAVA 480
           ALAAHASNPIEADRLRYLASPAGKDEYSQSVVGSQKSLLEVMAEFPSAKPPLGVFFAAVA
Sbjct: 421 ALAAHASNPIEADRLRYLASPAGKDEYSQSVVGSQKSLLEVMAEFPSAKPPLGVFFAAVA 480

Query: 481 PRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEKIHECSW 540
           PRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEKIHECSW
Sbjct: 481 PRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEKIHECSW 540

Query: 541 APIFVRQSNFKLPSDSKVPIIMVGPGTGLAPFRGFLQERLALKESGVELGPSILFFGCRN 600
           APIFVRQSNFKLPSDSKVPIIMVGPGTGLAPFRGFLQERLALKESGVELGPSILFFGCRN
Sbjct: 541 APIFVRQSNFKLPSDSKVPIIMVGPGTGLAPFRGFLQERLALKESGVELGPSILFFGCRN 600

Query: 601 RAMDYIYEDELNNFVETGALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGAYLYV 660
           RAMDYIYEDELNNFVETGALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGAYLYV
Sbjct: 601 RAMDYIYEDELNNFVETGALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGAYLYV 660

Query: 661 CGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVKNLQTSGRYLRDVW 708
           CGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVKNLQTSGRYLRDVW
Sbjct: 661 CGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVKNLQTSGRYLRDVW 708

BLAST of Cucsat.G18488.T1 vs. ExPASy TrEMBL
Match: A0A1S3BXG0 (NADPH--cytochrome P450 reductase OS=Cucumis melo OX=3656 GN=LOC103494263 PE=3 SV=1)

HSP 1 Score: 1396 bits (3614), Expect = 0.0
Identity = 692/708 (97.74%), Postives = 699/708 (98.73%), Query Frame = 0

Query: 1   MQSESSSMKASPFDFMSAIIKGRMDPSNSSFQSTGEGASVIFENRELVAILTTSIAVMIG 60
           MQSESSSMKASP DFMSAIIKGRMDPSNSSFQSTGEGASVIFENRELVAILTTSIAVMIG
Sbjct: 1   MQSESSSMKASPLDFMSAIIKGRMDPSNSSFQSTGEGASVIFENRELVAILTTSIAVMIG 60

Query: 61  CFVVLVWRRSGNRKVKTIELPKPLLGKEPEPEVDDGKKKVTIFFGTQTGTAEGFAKALSD 120
           CFVVLVWRRSGNRKVKTIELPKPLL KEPEPEVDDGKKKVTIFFGTQTGTAEGFAKALSD
Sbjct: 61  CFVVLVWRRSGNRKVKTIELPKPLLAKEPEPEVDDGKKKVTIFFGTQTGTAEGFAKALSD 120

Query: 121 EAKARYDKAKFRVVDLDDYGADEDEYEQKLKKESVAVFFLATYGDGEPTDNAARFYKWFT 180
           EAKARYDKAKFRVVDLDDYG D+DEYEQ+LKKESVAVFFLATYGDGEPTDNAARFYKWFT
Sbjct: 121 EAKARYDKAKFRVVDLDDYGGDDDEYEQQLKKESVAVFFLATYGDGEPTDNAARFYKWFT 180

Query: 181 EGKERGECLQNLNYAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQCIED 240
           EGKERGE LQNL+YAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQCIED
Sbjct: 181 EGKERGEWLQNLSYAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQCIED 240

Query: 241 DFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDPSDVTDDKKNWMNANGH 300
           DFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDP+DVTDD KNWMNANGH
Sbjct: 241 DFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDPADVTDDNKNWMNANGH 300

Query: 301 AVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDISESALKYETGDHVGVYCENLTETVD 360
           AVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDIS SALKYETGDHVGVYCENLTETVD
Sbjct: 301 AVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDISGSALKYETGDHVGVYCENLTETVD 360

Query: 361 EALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNSPKKSALL 420
           EALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNSPKKSALL
Sbjct: 361 EALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNSPKKSALL 420

Query: 421 ALAAHASNPIEADRLRYLASPAGKDEYSQSVVGSQKSLLEVMAEFPSAKPPLGVFFAAVA 480
           ALAAHASNPIEADRLRYLASP+GKDEYSQSVVG QKSLLEVMAEFPSAKPPLGVFFAAVA
Sbjct: 421 ALAAHASNPIEADRLRYLASPSGKDEYSQSVVGCQKSLLEVMAEFPSAKPPLGVFFAAVA 480

Query: 481 PRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEKIHECSW 540
           PRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEK HECSW
Sbjct: 481 PRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEKSHECSW 540

Query: 541 APIFVRQSNFKLPSDSKVPIIMVGPGTGLAPFRGFLQERLALKESGVELGPSILFFGCRN 600
           APIFVRQSNFKLPSDSKVPIIM+GPGTGLAPFRGFLQERLALKESGVELGPSILFFGCRN
Sbjct: 541 APIFVRQSNFKLPSDSKVPIIMIGPGTGLAPFRGFLQERLALKESGVELGPSILFFGCRN 600

Query: 601 RAMDYIYEDELNNFVETGALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGAYLYV 660
           RAMDYIYEDELNNFVE GALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGAYLYV
Sbjct: 601 RAMDYIYEDELNNFVEAGALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGAYLYV 660

Query: 661 CGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVKNLQTSGRYLRDVW 708
           CGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVK+LQTSGRYLRDVW
Sbjct: 661 CGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVKSLQTSGRYLRDVW 708

BLAST of Cucsat.G18488.T1 vs. ExPASy TrEMBL
Match: A0A5D3CN13 (NADPH--cytochrome P450 reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold152G00410 PE=3 SV=1)

HSP 1 Score: 1395 bits (3611), Expect = 0.0
Identity = 692/708 (97.74%), Postives = 698/708 (98.59%), Query Frame = 0

Query: 1   MQSESSSMKASPFDFMSAIIKGRMDPSNSSFQSTGEGASVIFENRELVAILTTSIAVMIG 60
           MQSESSSMKASP DFMSAIIKGRMDPSNSSFQSTGEGASVIFENRELVAILTTSIAVMIG
Sbjct: 1   MQSESSSMKASPLDFMSAIIKGRMDPSNSSFQSTGEGASVIFENRELVAILTTSIAVMIG 60

Query: 61  CFVVLVWRRSGNRKVKTIELPKPLLGKEPEPEVDDGKKKVTIFFGTQTGTAEGFAKALSD 120
           CFVVLVWRRSGNRKVKTIELPKPLL KEPEPEVDDGKKKVTIFFGTQTGTAEGFAKALSD
Sbjct: 61  CFVVLVWRRSGNRKVKTIELPKPLLAKEPEPEVDDGKKKVTIFFGTQTGTAEGFAKALSD 120

Query: 121 EAKARYDKAKFRVVDLDDYGADEDEYEQKLKKESVAVFFLATYGDGEPTDNAARFYKWFT 180
           EAKARYDKAKFRVVDLDDYG D+DEYEQ+LKKESVAVFFLATYGDGEPTDNAARFYKWFT
Sbjct: 121 EAKARYDKAKFRVVDLDDYGGDDDEYEQQLKKESVAVFFLATYGDGEPTDNAARFYKWFT 180

Query: 181 EGKERGECLQNLNYAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQCIED 240
           EGKERGE LQNL+YAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQCIED
Sbjct: 181 EGKERGEWLQNLSYAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQCIED 240

Query: 241 DFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDPSDVTDDKKNWMNANGH 300
           DFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDP+DVTDD KNWMNANGH
Sbjct: 241 DFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDPADVTDDNKNWMNANGH 300

Query: 301 AVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDISESALKYETGDHVGVYCENLTETVD 360
           AVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDIS SALKYETGDHVGVYCENLTETVD
Sbjct: 301 AVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDISGSALKYETGDHVGVYCENLTETVD 360

Query: 361 EALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNSPKKSALL 420
           EALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNSPKKSALL
Sbjct: 361 EALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNSPKKSALL 420

Query: 421 ALAAHASNPIEADRLRYLASPAGKDEYSQSVVGSQKSLLEVMAEFPSAKPPLGVFFAAVA 480
           ALAAHASNPIEADRLRYLASPAGKDEYSQSVVG QKSLLEVMAEFPSAKPPLGVFFAAVA
Sbjct: 421 ALAAHASNPIEADRLRYLASPAGKDEYSQSVVGCQKSLLEVMAEFPSAKPPLGVFFAAVA 480

Query: 481 PRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEKIHECSW 540
           PRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEK HECSW
Sbjct: 481 PRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEKSHECSW 540

Query: 541 APIFVRQSNFKLPSDSKVPIIMVGPGTGLAPFRGFLQERLALKESGVELGPSILFFGCRN 600
           APIFVRQSNFKLPSDSKVPIIM+GPGTGLAPFRGFLQERLALKESGVELGPSILFFGCRN
Sbjct: 541 APIFVRQSNFKLPSDSKVPIIMIGPGTGLAPFRGFLQERLALKESGVELGPSILFFGCRN 600

Query: 601 RAMDYIYEDELNNFVETGALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGAYLYV 660
           RAMDYIYEDELNNFVE GALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGAYLYV
Sbjct: 601 RAMDYIYEDELNNFVEAGALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGAYLYV 660

Query: 661 CGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVKNLQTSGRYLRDVW 708
           CGDAKGMARDVHRTLHTIVQEQGSLDS KAESMVK+LQTSGRYLRDVW
Sbjct: 661 CGDAKGMARDVHRTLHTIVQEQGSLDSLKAESMVKSLQTSGRYLRDVW 708

BLAST of Cucsat.G18488.T1 vs. ExPASy TrEMBL
Match: A0A5A7USX7 (NADPH--cytochrome P450 reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G006000 PE=3 SV=1)

HSP 1 Score: 1375 bits (3558), Expect = 0.0
Identity = 681/708 (96.19%), Postives = 691/708 (97.60%), Query Frame = 0

Query: 1   MQSESSSMKASPFDFMSAIIKGRMDPSNSSFQSTGEGASVIFENRELVAILTTSIAVMIG 60
           MQSESSSMKASP DFMSAIIKGRMDPSNSSFQSTGEGASVIFENRELVAILTTSIAVMIG
Sbjct: 1   MQSESSSMKASPLDFMSAIIKGRMDPSNSSFQSTGEGASVIFENRELVAILTTSIAVMIG 60

Query: 61  CFVVLVWRRSGNRKVKTIELPKPLLGKEPEPEVDDGKKKVTIFFGTQTGTAEGFAKALSD 120
           CFVVLVWRRSGNRKVKTIELPKPLL KEPEPEVDDGKKKVTIFFGTQTGTAEGFAKALSD
Sbjct: 61  CFVVLVWRRSGNRKVKTIELPKPLLAKEPEPEVDDGKKKVTIFFGTQTGTAEGFAKALSD 120

Query: 121 EAKARYDKAKFRVVDLDDYGADEDEYEQKLKKESVAVFFLATYGDGEPTDNAARFYKWFT 180
           EAKARYDKAKFRVVDLDDYG D+DEYEQ+LKKESVAVFFLATYGDGEPTDNAARFYKWFT
Sbjct: 121 EAKARYDKAKFRVVDLDDYGGDDDEYEQQLKKESVAVFFLATYGDGEPTDNAARFYKWFT 180

Query: 181 EGKERGECLQNLNYAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQCIED 240
           EGKERGE LQNL+YAVFGLGNRQYEHFNK++ V  + L    GKRLVKVGLGDDDQCIED
Sbjct: 181 EGKERGEWLQNLSYAVFGLGNRQYEHFNKVSMVPSKFLGVNCGKRLVKVGLGDDDQCIED 240

Query: 241 DFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDPSDVTDDKKNWMNANGH 300
           DFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDP+DVTDD KNWMNANGH
Sbjct: 241 DFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDPADVTDDNKNWMNANGH 300

Query: 301 AVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDISESALKYETGDHVGVYCENLTETVD 360
           AVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDIS SALKYETGDHVGVYCENLTETVD
Sbjct: 301 AVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDISGSALKYETGDHVGVYCENLTETVD 360

Query: 361 EALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNSPKKSALL 420
           EALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNSPKKSALL
Sbjct: 361 EALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNSPKKSALL 420

Query: 421 ALAAHASNPIEADRLRYLASPAGKDEYSQSVVGSQKSLLEVMAEFPSAKPPLGVFFAAVA 480
           ALAAHASNPIEADRLRYLASP+GKDEYSQSVVG QKSLLEVMAEFPSAKPPLGVFFAAVA
Sbjct: 421 ALAAHASNPIEADRLRYLASPSGKDEYSQSVVGCQKSLLEVMAEFPSAKPPLGVFFAAVA 480

Query: 481 PRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEKIHECSW 540
           PRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEK HECSW
Sbjct: 481 PRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEKSHECSW 540

Query: 541 APIFVRQSNFKLPSDSKVPIIMVGPGTGLAPFRGFLQERLALKESGVELGPSILFFGCRN 600
           APIFVRQSNFKLPSDSKVPIIM+GPGTGLAPFRGFLQERLALKESGVELGPSILFFGCRN
Sbjct: 541 APIFVRQSNFKLPSDSKVPIIMIGPGTGLAPFRGFLQERLALKESGVELGPSILFFGCRN 600

Query: 601 RAMDYIYEDELNNFVETGALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGAYLYV 660
           RAMDYIYEDELNNFVE GALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGAYLYV
Sbjct: 601 RAMDYIYEDELNNFVEAGALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGAYLYV 660

Query: 661 CGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVKNLQTSGRYLRDVW 708
           CGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVK+LQTSGRYLRDVW
Sbjct: 661 CGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVKSLQTSGRYLRDVW 708

BLAST of Cucsat.G18488.T1 vs. ExPASy TrEMBL
Match: A0A6J1JGV7 (NADPH--cytochrome P450 reductase OS=Cucurbita maxima OX=3661 GN=LOC111484987 PE=3 SV=1)

HSP 1 Score: 1337 bits (3461), Expect = 0.0
Identity = 660/708 (93.22%), Postives = 679/708 (95.90%), Query Frame = 0

Query: 1   MQSESSSMKASPFDFMSAIIKGRMDPSNSSFQSTGEGASVIFENRELVAILTTSIAVMIG 60
           MQSESSSMKASPF+FMSAIIKGRMDPSNSSFQST EGASV+FENRELVAILTTSIAVMIG
Sbjct: 1   MQSESSSMKASPFEFMSAIIKGRMDPSNSSFQSTAEGASVMFENRELVAILTTSIAVMIG 60

Query: 61  CFVVLVWRRSGNRKVKTIELPKPLLGKEPEPEVDDGKKKVTIFFGTQTGTAEGFAKALSD 120
           CFVVLVWRRSGNRKVKT+ELPKPL+ K  EPEV+DGKKKVTIFFGTQTGTAEGFAKALSD
Sbjct: 61  CFVVLVWRRSGNRKVKTVELPKPLITKGVEPEVEDGKKKVTIFFGTQTGTAEGFAKALSD 120

Query: 121 EAKARYDKAKFRVVDLDDYGADEDEYEQKLKKESVAVFFLATYGDGEPTDNAARFYKWFT 180
           EAKARYDKA FRVVDLDDYGAD+DEYEQKLK ES+AVFFLATYGDGEPTDNAARFYKWFT
Sbjct: 121 EAKARYDKAAFRVVDLDDYGADDDEYEQKLKNESIAVFFLATYGDGEPTDNAARFYKWFT 180

Query: 181 EGKERGECLQNLNYAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQCIED 240
           EGKERGE LQNLNYAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQCIED
Sbjct: 181 EGKERGEWLQNLNYAVFGLGNRQYEHFNKIAKVVDELLETQGGKRLVKVGLGDDDQCIED 240

Query: 241 DFSAWRESLWPELDQLLRDEDDAATVTTPYTAAISEYRVVFHDPSDVTDDKKNWMNANGH 300
           D +AWRE LWPELDQLLRDEDDA TVTTPYTAAISEYRVVFHDP+ V D+ KNWMNANGH
Sbjct: 241 DLAAWRELLWPELDQLLRDEDDATTVTTPYTAAISEYRVVFHDPAGVADENKNWMNANGH 300

Query: 301 AVHDAQHPFRSNVVVRKELHTPASDRSCTHLEFDISESALKYETGDHVGVYCENLTETVD 360
           AVHDAQHP RSNVVVRKELH PASDRSCTHLEFDIS SALKYETGDHVGVYCENLTETVD
Sbjct: 301 AVHDAQHPLRSNVVVRKELHMPASDRSCTHLEFDISGSALKYETGDHVGVYCENLTETVD 360

Query: 361 EALNLLGLSPETYFSIHTDNEDGTQLGGSSLPPPFPSCTLRTALTRYADLLNSPKKSALL 420
           EALNLLGLSPETYFSIHTDNEDGT L G+SLPPPFPSCTLRTAL RYADLLNSPKKSALL
Sbjct: 361 EALNLLGLSPETYFSIHTDNEDGTPLVGNSLPPPFPSCTLRTALARYADLLNSPKKSALL 420

Query: 421 ALAAHASNPIEADRLRYLASPAGKDEYSQSVVGSQKSLLEVMAEFPSAKPPLGVFFAAVA 480
           ALAAHASNP+EADRLRYLASPAGKDEYSQS+VG QKSLLEVM EFPSAKPPLGVFFAAVA
Sbjct: 421 ALAAHASNPVEADRLRYLASPAGKDEYSQSIVGCQKSLLEVMVEFPSAKPPLGVFFAAVA 480

Query: 481 PRLQPRFYSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGICSTWMKNSVPMEKIHECSW 540
           PRLQPRFYSISSSP MAPSRIHVTCALVYDKMPTGR+HKG+CSTWMKNSVPM K  ECSW
Sbjct: 481 PRLQPRFYSISSSPLMAPSRIHVTCALVYDKMPTGRVHKGVCSTWMKNSVPMGKSDECSW 540

Query: 541 APIFVRQSNFKLPSDSKVPIIMVGPGTGLAPFRGFLQERLALKESGVELGPSILFFGCRN 600
           APIFVRQSNFKLP+DSKVPIIMVGPGTGLAPFRGFLQERLALKESGV+LG SILFFGCRN
Sbjct: 541 APIFVRQSNFKLPADSKVPIIMVGPGTGLAPFRGFLQERLALKESGVQLGSSILFFGCRN 600

Query: 601 RAMDYIYEDELNNFVETGALSELVIAFSREGPTKEYVQHKMTEKASDIWNLISQGAYLYV 660
           R MDYIYEDELNN++ETGALSELVIAFSREGPTKEYVQHKM EKASDIWNLISQGAYLYV
Sbjct: 601 RGMDYIYEDELNNYLETGALSELVIAFSREGPTKEYVQHKMAEKASDIWNLISQGAYLYV 660

Query: 661 CGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVKNLQTSGRYLRDVW 708
           CGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVKNLQ SGRYLRDVW
Sbjct: 661 CGDAKGMARDVHRTLHTIVQEQGSLDSSKAESMVKNLQMSGRYLRDVW 708

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A2U1LIM90.0e+0074.75NADPH--cytochrome P450 reductase 1 OS=Artemisia annua OX=35608 GN=CPR1 PE=1 SV=2[more]
Q9SUM30.0e+0075.00NADPH--cytochrome P450 reductase 2 OS=Arabidopsis thaliana OX=3702 GN=ATR2 PE=1 ... [more]
Q050010.0e+0074.41NADPH--cytochrome P450 reductase OS=Catharanthus roseus OX=4058 GN=CPR PE=2 SV=1[more]
A0A2U1KZS61.4e-30371.03NADPH--cytochrome P450 reductase 2 OS=Artemisia annua OX=35608 GN=CPR2 PE=3 SV=1[more]
Q653S92.8e-29970.42NADPH--cytochrome P450 reductase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=C... [more]
Match NameE-valueIdentityDescription
XP_004146317.10.0100.00NADPH--cytochrome P450 reductase 2 [Cucumis sativus] >AIT72028.1 cytochrome P450... [more]
XP_008453604.10.097.74PREDICTED: NADPH--cytochrome P450 reductase 2 [Cucumis melo][more]
TYK11826.10.097.74NADPH--cytochrome P450 reductase 2 [Cucumis melo var. makuwa][more]
XP_038879587.10.095.76NADPH--cytochrome P450 reductase 1-like [Benincasa hispida][more]
KAA0058274.10.096.19NADPH--cytochrome P450 reductase 2 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
A0A0A0LXA10.0100.00NADPH--cytochrome P450 reductase OS=Cucumis sativus OX=3659 GN=Csa1G423150 PE=2 ... [more]
A0A1S3BXG00.097.74NADPH--cytochrome P450 reductase OS=Cucumis melo OX=3656 GN=LOC103494263 PE=3 SV... [more]
A0A5D3CN130.097.74NADPH--cytochrome P450 reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A5A7USX70.096.19NADPH--cytochrome P450 reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A6J1JGV70.093.22NADPH--cytochrome P450 reductase OS=Cucurbita maxima OX=3661 GN=LOC111484987 PE=... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001094Flavodoxin-likePRINTSPR00369FLAVODOXINcoord: 215..234
score: 43.59
coord: 191..201
score: 61.08
coord: 101..114
score: 53.46
coord: 157..168
score: 49.09
IPR001709Flavoprotein pyridine nucleotide cytochrome reductasePRINTSPR00371FPNCRcoord: 590..599
score: 44.25
coord: 603..614
score: 36.69
coord: 561..580
score: 61.78
coord: 342..352
score: 40.02
coord: 486..493
score: 68.1
coord: 634..650
score: 33.33
coord: 520..529
score: 36.78
coord: 657..665
score: 49.04
IPR023173NADPH-cytochrome p450 reductase, FAD-binding, alpha-helical domain superfamilyGENE3D1.20.990.10coord: 352..484
e-value: 3.5E-87
score: 293.8
IPR003097Sulfite reductase [NADPH] flavoprotein alpha-component-like, FAD-bindingPFAMPF00667FAD_binding_1coord: 304..525
e-value: 7.9E-68
score: 228.3
IPR023208NADPH-cytochrome P450 reductasePIRSFPIRSF000208P450Rcoord: 48..708
e-value: 5.4E-192
score: 637.5
IPR023208NADPH-cytochrome P450 reductaseHAMAPMF_03212NCPRcoord: 40..708
score: 31.670321
IPR029039Flavoprotein-like superfamilyGENE3D3.40.50.360coord: 85..268
e-value: 2.0E-53
score: 182.5
IPR029039Flavoprotein-like superfamilySUPERFAMILY52218Flavoproteinscoord: 92..257
IPR039261Ferredoxin-NADP reductase (FNR), nucleotide-binding domainGENE3D3.40.50.80coord: 552..708
e-value: 4.2E-57
score: 194.4
IPR039261Ferredoxin-NADP reductase (FNR), nucleotide-binding domainSUPERFAMILY52343Ferredoxin reductase-like, C-terminal NADP-linked domaincoord: 549..708
IPR008254Flavodoxin/nitric oxide synthasePFAMPF00258Flavodoxin_1coord: 102..245
e-value: 2.5E-32
score: 112.1
IPR008254Flavodoxin/nitric oxide synthasePROSITEPS50902FLAVODOXIN_LIKEcoord: 100..250
score: 33.041893
IPR001433Oxidoreductase FAD/NAD(P)-bindingPFAMPF00175NAD_binding_1coord: 562..672
e-value: 1.4E-14
score: 54.6
NoneNo IPR availableGENE3D2.40.30.10Translation factorscoord: 308..551
e-value: 3.5E-87
score: 293.8
NoneNo IPR availablePIRSRPIRSR000207-1PIRSR000207-1coord: 99..708
e-value: 1.8E-139
score: 463.6
NoneNo IPR availablePANTHERPTHR19384NITRIC OXIDE SYNTHASE-RELATEDcoord: 12..708
NoneNo IPR availablePANTHERPTHR19384:SF119NADPH--CYTOCHROME P450 REDUCTASEcoord: 12..708
NoneNo IPR availableCDDcd06204CYPORcoord: 304..708
e-value: 0.0
score: 594.235
IPR017927FAD-binding domain, ferredoxin reductase-typePROSITEPS51384FAD_FRcoord: 306..553
score: 13.674505
IPR017938Riboflavin synthase-like beta-barrelSUPERFAMILY63380Riboflavin synthase domain-likecoord: 294..550

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G18488Cucsat.G18488gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G18488.T1.E1Cucsat.G18488.T1.E1exon
Cucsat.G18488.T1.E2Cucsat.G18488.T1.E2exon
Cucsat.G18488.T1.E3Cucsat.G18488.T1.E3exon
Cucsat.G18488.T1.E4Cucsat.G18488.T1.E4exon
Cucsat.G18488.T1.E5Cucsat.G18488.T1.E5exon
Cucsat.G18488.T1.E6Cucsat.G18488.T1.E6exon
Cucsat.G18488.T1.E7Cucsat.G18488.T1.E7exon
Cucsat.G18488.T1.E8Cucsat.G18488.T1.E8exon
Cucsat.G18488.T1.E9Cucsat.G18488.T1.E9exon
Cucsat.G18488.T1.E10Cucsat.G18488.T1.E10exon
Cucsat.G18488.T1.E11Cucsat.G18488.T1.E11exon
Cucsat.G18488.T1.E12Cucsat.G18488.T1.E12exon
Cucsat.G18488.T1.E13Cucsat.G18488.T1.E13exon
Cucsat.G18488.T1.E14Cucsat.G18488.T1.E14exon
Cucsat.G18488.T1.E15Cucsat.G18488.T1.E15exon
Cucsat.G18488.T1.E16Cucsat.G18488.T1.E16exon
Cucsat.G18488.T1.E17Cucsat.G18488.T1.E17exon
Cucsat.G18488.T1.E18Cucsat.G18488.T1.E18exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G18488.T1.C1Cucsat.G18488.T1.C1CDS
Cucsat.G18488.T1.C2Cucsat.G18488.T1.C2CDS
Cucsat.G18488.T1.C3Cucsat.G18488.T1.C3CDS
Cucsat.G18488.T1.C4Cucsat.G18488.T1.C4CDS
Cucsat.G18488.T1.C5Cucsat.G18488.T1.C5CDS
Cucsat.G18488.T1.C6Cucsat.G18488.T1.C6CDS
Cucsat.G18488.T1.C7Cucsat.G18488.T1.C7CDS
Cucsat.G18488.T1.C8Cucsat.G18488.T1.C8CDS
Cucsat.G18488.T1.C9Cucsat.G18488.T1.C9CDS
Cucsat.G18488.T1.C10Cucsat.G18488.T1.C10CDS
Cucsat.G18488.T1.C11Cucsat.G18488.T1.C11CDS
Cucsat.G18488.T1.C12Cucsat.G18488.T1.C12CDS
Cucsat.G18488.T1.C13Cucsat.G18488.T1.C13CDS
Cucsat.G18488.T1.C14Cucsat.G18488.T1.C14CDS
Cucsat.G18488.T1.C15Cucsat.G18488.T1.C15CDS
Cucsat.G18488.T1.C16Cucsat.G18488.T1.C16CDS
Cucsat.G18488.T1.C17Cucsat.G18488.T1.C17CDS
Cucsat.G18488.T1.C18Cucsat.G18488.T1.C18CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G18488.T1Cucsat.G18488.T1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005829 cytosol
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0010181 FMN binding
molecular_function GO:0003958 NADPH-hemoprotein reductase activity
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0050661 NADP binding