Cucsat.G16818.T25 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G16818.T25
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionUbiquitin thioesterase
Locationctg24: 1640708 .. 1644456 (-)
RNA-Seq ExpressionCucsat.G16818.T25
SyntenyCucsat.G16818.T25
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAGCTTCATGTTTTCTTATCTTGTAAGTTTAATTCTTTTAATTTATCTCATTTACTTTGCTATTATACTTTTTGTGCATCATCGAGCATCAGCTGTTACTGACTTGGCCTCTCAAATCAGGAACACATTTTAGAGTCACAAGACAAAACTGAAGTTGATCGCATCAAAACAAATGTTGAAAATTGTAGGAAAACACTTCGAAGTCTGGGATATACAGAATTTACCTTTGAAGATTTTTTTGCGGTAAATTCCTACCATTCCTTTTTTTTTTAAAAAAAAACAATTAATCTATTGCACATTATCGCCCTATGCTCTAAGCAGTATGGCTTATTTCTTCCATTCAACTGGAATTGGTGGAAGTTGAGAACTATTCATACTAGTCTCTAAATGTGTTATTGAACATACCATATATAAATATTGTGTAAGATACTTAATTAGGGAGATTGGTATGGTATTAGTAAGGGGCATAAGGGCAATTAGATTGGGAGTTGATGTATCTTGTTATAAATAGGAGAGTCAGTGAGGGAAGGTGCCCAGCAATTGTCGAGTGTTATAGGGCTTGGGATGCCATACTCAAGAGGAAGGTTCCAAGTGCCTTATATTGGATTATCTCGTAGTTCCATCTTTATCAATTTTATTGTTGTTGAGATGTTTTCTTTGCAGTTTGTGATGGTATAGTAGGTTTATTTTCCCTTTTTTGAACCTTTCCATCTAAGTATGTGCTTTCTCTCCAATTGATGGATATGAATGAGGAAATTAGACCAAACTAAAGAGGACTATAGGGGGCTGCAAGTTCAATGATGGAAAGGTGGTGATGAGAATCTATTAATGAAATTGGCCCCTCCACTTTGTAATTTCATCCATTAATGAAATTGTCTCTTAACAAAAAGAAAAAAAGTGGTGATGAGAATCTATTTTTTGTGATGCCAGATTTAACATTAACGTCATTGCAATGACATCTCATATTGAATTAACTTTGAATACGAACATTTTGTATATTATGTCTTTACCTCATCGAAGTCCAGTCTTTTTCATAACTCATATATAGACTCTAGAGCTATTCTTTAAAAATGTGTGGACCAACTTTTAAATACATTGTTCCAGATTTATACTACCTTAAAGATGTCTAGAGCCTAGACTCGTCCTTCTTTATTATTGCTGTCTTTGTTTCATTTTGGTATAAACTGGCTAGATATCCTTGGTGGTTAATGATGGGTGCCATAAATATAAATTATTTATTAATAAATATGTTTAAAATATCTTTACAGCTATTCCTTGAGCAGCTGGAAAGTGCTCTTCAAGGAAATGAATCCTCTATAAGGTGTCAATGACCTGCCCTTCTACCTTGGAATTTTGATTCCTGAATTTTTAGTTGATAGTGCAATTTTCTGTTGCCATCAATTATGAAATTTTGCCATTCATGATTGCATTTGACTCTTATTTTTCTTGGATGCAGTCATGAAGAACTTGTTATTAGGAGTCGGGACCAGTCTATCTCTGATTATGGTCAGTGATGTTTTATATTCAATATTGAATTCTCTCTTTCCATTTCTACTGACAAAATTGTTTTTCTAGTTGTTATGTTTTTCAGGTTTGTTACATCAGGTGAAATACAAAAGCGTTCAGAATTTTTTGAGCCTTTTATTATGGGATTGACAAATGGAACGGTTGATCAGGTTTCCTAAATTTTTAACCCCTATAAGTTTTGCATTATGTTTTGACTTTTATCTTTAGATTTATGTTGGAAATTCAACCCTACAAGATATTTGAAATCTAAGCTGATTTGGCTAGGTGAACCCTTTTACAAGTGTAGTTTCAAGCAGAAGCAGCTAATTGTTATAAAGCAATAATGATGATTTAGACCATGTATACCTTGGACAGGAATAAGAATGGATATTTACGATTTCCGCTCTTGTTTACTTCTTCTTGGGATTGGAATAAGTATAACCCACTTAAGAATGTAGAACCTTGCTCTTATTTTGGAGCAACATCTTTACTTCATAATAGATAACCATTCTCTCTCCTATTGCTGTTCTATGGGTTCAAAGTCTTTTCTGTGGGTCTGTTGACCTTTTTTCCTCATTCTAAAAATCTAAATGTTAACAAACAATATCGTATCAACCACCGTTTTCTTATTCCAGACTTCATAATAATTGTAGTAAACAAAGTTTCAGAAATTCTATTTCAAATCCTTTTTTTTTTTTCTATTCAATCAGACATGATGGATTGCCATATGGTTTTATTCCCTCATTAAATAAACTAAGCCTTGGGTTCATGATTTTATCATCACATTTATATTTAAATGAAATCACTTTAGCCTTATTATTTCCCTTTTTTTGAGTGCACATGTGGGGATTTAAACTTTTCAACCTTGTTTCCATTTCAAAAGAAAAAAAAAAAAAATTTAAACTTTTGACCTTTTGGTCTGCTATTATTCCCTTTTTGGTGGATCAGAATCCCAAACAACAGTAAGCCTTGGTCTTTGTGTTTGGGGGTTAACTTTTCCAAGGGTTGGTCACTTATGATGATAATTTTTAGTTTTTGGTTTGGTGGGGAAACTAGACATAGACCTGAACATGGTTGGACTCGTATTAACTTTGTAAAAAGTCAAATGAACATTTAATTTCTTATTTAAGAAGAAAGAGAACCTATTTATCATGACTTCCAATCTCATCCTCTTTGGCACAGTTCTGCAAGACAGCAGTTGAACCAATGGGTGAAGAGAGTGATCATGTACACATAATTGCCTTATCTGATGCTTTGGGTGTGCCGATTCGTGTTCTGTACCTTGATCGAAGCTCCTGTGATTCTGGTGGCCTCAGTGTAAACCACCATGATTTTGTTCCTGCTACCACTGAGGTCGCAAGTGGTAGTGCTGCCTCTGAGATAAAGATCCCTTTCATTACTTTGCTCTATCGCCCTGGTCATTATGATATCCTTTATCCAAAATGATGGTAAACTTCTCTCACGAACTTATTGGAAAATTTTCCATAGAAATATCACTTCTTCCCCACCTATTATATGCAACAGATCAAATTTAATCTTTATAAAATGTTGATGTTTAAGAATTAAGAGTATATTATGGCTGCCATATCCTCATTCCGCATGCAAAAATGATATTTTCATCTGTAGTTGTCTACCGTTATTTGCAAATTTGTTCAAGTCTTTGATTCACTTGAGGATTTTTGTTCTGGGTGGTTCAGCTTGAATTATATGTTATTTATTTCAGGGTGGTGGGGCTCTCAGAGTTGAACTGCATTGACACGGCGGACATGGATCCAAGCTTCGACAAGATTAAAGGTTGGATCTTCATAATTGCTAGATGTTGTTCTGTACTGATTTTAATTACTGATGAGCTTGTTTGAGTAAAAAAAGAAAGGAATTATATTCACCAGTTAACTGAAATTTGAAATATACATGTGAATCAGTCTTCGAGTTTTTCAGTCATCTTGCAAATAGATTTCCTTACACAATCTCTTAGAATGTTTGAGAATTGAGATATAGTATTTCAATCCAATTTCAATACAGGGACACTATTACTTGGGGTGATGTTCTTAAATTCTAATAACTTACAATTCAACCCTAGGATTTAGAGAAGTTCATATTTGTATATGTACATTTTGAGCCAAAATGGATGCATCTTGCTATGATTTCAAAAATATATTAGTAGCAGAACATATTCTCAGTGATTGAAGTTTTTGGTGTATTAATGTCTAAAAGGGCAGTACAAGCATTATTATTTGGTGAC

Coding sequence (CDS)

AGCTTCATGTTTTCTTATCTTCTATTCCTTGAGCAGCTGGAAAGTGCTCTTCAAGGAAATGAATCCTCTATAAGTCATGAAGAACTTGTTATTAGGAGTCGGGACCAGTCTATCTCTGATTATGTTGTTATGTTTTTCAGGTTTGTTACATCAGGTGAAATACAAAAGCGTTCAGAATTTTTTGAGCCTTTTATTATGGGATTGACAAATGGAACGGTTGATCAGTTCTGCAAGACAGCAGTTGAACCAATGGGTGAAGAGAGTGATCATGTACACATAATTGCCTTATCTGATGCTTTGGGTGTGCCGATTCGTGTTCTGTACCTTGATCGAAGCTCCTGTGATTCTGGTGGCCTCAGTGTAAACCACCATGATTTTGTTCCTGCTACCACTGAGGTCGCAAGTGGTAGTGCTGCCTCTGAGATAAAGATCCCTTTCATTACTTTGCTCTATCGCCCTGGTCATTATGATATCCTTTATCCAAAATGA

Protein sequence

SFMFSYLLFLEQLESALQGNESSISHEELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVASGSAASEIKIPFITLLYRPGHYDILYPK
Homology
BLAST of Cucsat.G16818.T25 vs. ExPASy Swiss-Prot
Match: Q8LG98 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 1 OS=Arabidopsis thaliana OX=3702 GN=OTU1 PE=1 SV=2)

HSP 1 Score: 235.7 bits (600), Expect = 3.6e-61
Identity = 113/157 (71.97%), Postives = 132/157 (84.08%), Query Frame = 0

Query: 6   YLLFLEQLESALQGNESSISHEELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFI 65
           + LFLEQL+  LQG E SIS++ELV RSRDQS+SDY+VMFFRFVT+G+I+ R++FFEPFI
Sbjct: 142 FALFLEQLDDILQGTEESISYDELVNRSRDQSVSDYIVMFFRFVTAGDIRTRADFFEPFI 201

Query: 66  MGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSVNHHD 125
            GL+N TVDQFCK++VEPMGEESDH+HI ALSDALGV IRV+YLDRSSCDSGG++VNHHD
Sbjct: 202 TGLSNATVDQFCKSSVEPMGEESDHIHITALSDALGVAIRVVYLDRSSCDSGGVTVNHHD 261

Query: 126 FVPATTEVASGSAASEIKIPFITLLYRPGHYDILYPK 163
           FVP      +     E   PFITLLYRPGHYDILYPK
Sbjct: 262 FVPVG---ITNEKDEEASAPFITLLYRPGHYDILYPK 295

BLAST of Cucsat.G16818.T25 vs. ExPASy Swiss-Prot
Match: Q96FW1 (Ubiquitin thioesterase OTUB1 OS=Homo sapiens OX=9606 GN=OTUB1 PE=1 SV=2)

HSP 1 Score: 111.3 bits (277), Expect = 1.0e-23
Identity = 66/140 (47.14%), Postives = 85/140 (60.71%), Query Frame = 0

Query: 21  ESSISHEELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTA 80
           E   S  +L+    DQS SDY+V++ R +TSG +Q+ S+FFE FI G    TV +FC+  
Sbjct: 150 EKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEG--GRTVKEFCQQE 209

Query: 81  VEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVASGSAAS 140
           VEPM +ESDH+HIIAL+ AL V I+V Y+DR      G + N H F P  +E        
Sbjct: 210 VEPMCKESDHIHIIALAQALSVSIQVEYMDRGE----GGTTNPHIF-PEGSE-------- 269

Query: 141 EIKIPFITLLYRPGHYDILY 161
               P + LLYRPGHYDILY
Sbjct: 270 ----PKVYLLYRPGHYDILY 270

BLAST of Cucsat.G16818.T25 vs. ExPASy Swiss-Prot
Match: Q7TQI3 (Ubiquitin thioesterase OTUB1 OS=Mus musculus OX=10090 GN=Otub1 PE=1 SV=2)

HSP 1 Score: 111.3 bits (277), Expect = 1.0e-23
Identity = 66/140 (47.14%), Postives = 85/140 (60.71%), Query Frame = 0

Query: 21  ESSISHEELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTA 80
           E   S  +L+    DQS SDY+V++ R +TSG +Q+ S+FFE FI G    TV +FC+  
Sbjct: 150 EKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEG--GRTVKEFCQQE 209

Query: 81  VEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVASGSAAS 140
           VEPM +ESDH+HIIAL+ AL V I+V Y+DR      G + N H F P  +E        
Sbjct: 210 VEPMCKESDHIHIIALAQALSVSIQVEYMDRGE----GGTTNPHVF-PEGSE-------- 269

Query: 141 EIKIPFITLLYRPGHYDILY 161
               P + LLYRPGHYDILY
Sbjct: 270 ----PKVYLLYRPGHYDILY 270

BLAST of Cucsat.G16818.T25 vs. ExPASy Swiss-Prot
Match: B2RYG6 (Ubiquitin thioesterase OTUB1 OS=Rattus norvegicus OX=10116 GN=Otub1 PE=1 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.0e-23
Identity = 66/140 (47.14%), Postives = 85/140 (60.71%), Query Frame = 0

Query: 21  ESSISHEELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTA 80
           E   S  +L+    DQS SDY+V++ R +TSG +Q+ S+FFE FI G    TV +FC+  
Sbjct: 150 EKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEG--GRTVKEFCQQE 209

Query: 81  VEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVASGSAAS 140
           VEPM +ESDH+HIIAL+ AL V I+V Y+DR      G + N H F P  +E        
Sbjct: 210 VEPMCKESDHIHIIALAQALSVSIQVEYMDRGE----GGTTNPHVF-PEGSE-------- 269

Query: 141 EIKIPFITLLYRPGHYDILY 161
               P + LLYRPGHYDILY
Sbjct: 270 ----PKVYLLYRPGHYDILY 270

BLAST of Cucsat.G16818.T25 vs. ExPASy Swiss-Prot
Match: Q9VL00 (Ubiquitin thioesterase otubain-like OS=Drosophila melanogaster OX=7227 GN=CG4968 PE=2 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 5.6e-22
Identity = 65/160 (40.62%), Postives = 95/160 (59.38%), Query Frame = 0

Query: 2   FMFSYLLFLEQLESALQGNESSISHEELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFF 61
           F  +++  ++++     G  S++  +EL     +Q  SDYVV++ R +TSG++Q+ ++F+
Sbjct: 122 FHETFMEVIQRVSPDNAGGHSTV-QDELHKIFNEQGYSDYVVVYLRLITSGKLQEEADFY 181

Query: 62  EPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSV 121
           + FI G  + T++ F    VEPM +ESDH+HIIAL  ALG  +RV YLDR      G +V
Sbjct: 182 QNFIEG--DLTIEAFRHLEVEPMYKESDHIHIIALCTALGAGVRVEYLDRGE----GGTV 241

Query: 122 NHHDFVPATTEVASGSAASEIKIPFITLLYRPGHYDILYP 162
             HDF P  +E            P I L+YRPGHYDILYP
Sbjct: 242 KAHDF-PEGSE------------PRIYLIYRPGHYDILYP 261

BLAST of Cucsat.G16818.T25 vs. NCBI nr
Match: XP_004152159.1 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 1 [Cucumis sativus] >XP_011653009.1 OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 1 [Cucumis sativus] >KGN52967.1 hypothetical protein Csa_014673 [Cucumis sativus])

HSP 1 Score: 310 bits (795), Expect = 2.97e-104
Identity = 155/157 (98.73%), Postives = 156/157 (99.36%), Query Frame = 0

Query: 6   YLLFLEQLESALQGNESSISHEELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFI 65
           + LFLEQLESALQGNESSISHEELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFI
Sbjct: 146 FALFLEQLESALQGNESSISHEELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFI 205

Query: 66  MGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSVNHHD 125
           MGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSVNHHD
Sbjct: 206 MGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSVNHHD 265

Query: 126 FVPATTEVASGSAASEIKIPFITLLYRPGHYDILYPK 162
           FVPATTEVASGSAASEIKIPFITLLYRPGHYDILYPK
Sbjct: 266 FVPATTEVASGSAASEIKIPFITLLYRPGHYDILYPK 302

BLAST of Cucsat.G16818.T25 vs. NCBI nr
Match: XP_008454143.1 (PREDICTED: ubiquitin thioesterase otubain-like [Cucumis melo] >XP_008454144.1 PREDICTED: ubiquitin thioesterase otubain-like [Cucumis melo])

HSP 1 Score: 305 bits (782), Expect = 2.81e-102
Identity = 152/157 (96.82%), Postives = 155/157 (98.73%), Query Frame = 0

Query: 6   YLLFLEQLESALQGNESSISHEELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFI 65
           + LFLEQLESALQGNESSISH+ELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFI
Sbjct: 146 FALFLEQLESALQGNESSISHDELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFI 205

Query: 66  MGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSVNHHD 125
           MGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSVNHHD
Sbjct: 206 MGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSVNHHD 265

Query: 126 FVPATTEVASGSAASEIKIPFITLLYRPGHYDILYPK 162
           FVPATTEV+S SAASEIKIPFITLLYRPGHYDILYPK
Sbjct: 266 FVPATTEVSSDSAASEIKIPFITLLYRPGHYDILYPK 302

BLAST of Cucsat.G16818.T25 vs. NCBI nr
Match: TYK29632.1 (ubiquitin thioesterase otubain-like [Cucumis melo var. makuwa])

HSP 1 Score: 305 bits (782), Expect = 5.81e-102
Identity = 152/157 (96.82%), Postives = 155/157 (98.73%), Query Frame = 0

Query: 6   YLLFLEQLESALQGNESSISHEELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFI 65
           + LFLEQLESALQGNESSISH+ELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFI
Sbjct: 142 FALFLEQLESALQGNESSISHDELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFI 201

Query: 66  MGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSVNHHD 125
           MGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSVNHHD
Sbjct: 202 MGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSVNHHD 261

Query: 126 FVPATTEVASGSAASEIKIPFITLLYRPGHYDILYPK 162
           FVPATTEV+S SAASEIKIPFITLLYRPGHYDILYPK
Sbjct: 262 FVPATTEVSSDSAASEIKIPFITLLYRPGHYDILYPK 298

BLAST of Cucsat.G16818.T25 vs. NCBI nr
Match: KAA0044504.1 (ubiquitin thioesterase otubain-like [Cucumis melo var. makuwa])

HSP 1 Score: 305 bits (782), Expect = 5.81e-102
Identity = 152/157 (96.82%), Postives = 155/157 (98.73%), Query Frame = 0

Query: 6   YLLFLEQLESALQGNESSISHEELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFI 65
           + LFLEQLESALQGNESSISH+ELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFI
Sbjct: 142 FALFLEQLESALQGNESSISHDELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFI 201

Query: 66  MGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSVNHHD 125
           MGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSVNHHD
Sbjct: 202 MGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSVNHHD 261

Query: 126 FVPATTEVASGSAASEIKIPFITLLYRPGHYDILYPK 162
           FVPATTEV+S SAASEIKIPFITLLYRPGHYDILYPK
Sbjct: 262 FVPATTEVSSDSAASEIKIPFITLLYRPGHYDILYPK 298

BLAST of Cucsat.G16818.T25 vs. NCBI nr
Match: XP_022145855.1 (ubiquitin thioesterase otubain-like isoform X2 [Momordica charantia])

HSP 1 Score: 302 bits (773), Expect = 1.66e-101
Identity = 147/162 (90.74%), Postives = 158/162 (97.53%), Query Frame = 0

Query: 1   SFMFSYLLFLEQLESALQGNESSISHEELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEF 60
           SFMFSYLLFLEQLESALQGNE+SISH+ELV+RSRDQSISDYVVMFFRFV+SGEIQKRSEF
Sbjct: 100 SFMFSYLLFLEQLESALQGNETSISHDELVLRSRDQSISDYVVMFFRFVSSGEIQKRSEF 159

Query: 61  FEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLS 120
           FEPFIMGLTNGTVDQFCK+AVEPMGEESDHVHIIALSDALGVPIRV+YLDRSSCDSGG+S
Sbjct: 160 FEPFIMGLTNGTVDQFCKSAVEPMGEESDHVHIIALSDALGVPIRVIYLDRSSCDSGGVS 219

Query: 121 VNHHDFVPATTEVASGSAASEIKIPFITLLYRPGHYDILYPK 162
           VNHHDFVPA ++V+S  A SEIKIP+ITLLYRPGHYDILYPK
Sbjct: 220 VNHHDFVPAASDVSSDGAGSEIKIPYITLLYRPGHYDILYPK 261

BLAST of Cucsat.G16818.T25 vs. ExPASy TrEMBL
Match: A0A0A0KX37 (Ubiquitin thioesterase OS=Cucumis sativus OX=3659 GN=Csa_4G007720 PE=3 SV=1)

HSP 1 Score: 310 bits (795), Expect = 1.44e-104
Identity = 155/157 (98.73%), Postives = 156/157 (99.36%), Query Frame = 0

Query: 6   YLLFLEQLESALQGNESSISHEELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFI 65
           + LFLEQLESALQGNESSISHEELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFI
Sbjct: 146 FALFLEQLESALQGNESSISHEELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFI 205

Query: 66  MGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSVNHHD 125
           MGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSVNHHD
Sbjct: 206 MGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSVNHHD 265

Query: 126 FVPATTEVASGSAASEIKIPFITLLYRPGHYDILYPK 162
           FVPATTEVASGSAASEIKIPFITLLYRPGHYDILYPK
Sbjct: 266 FVPATTEVASGSAASEIKIPFITLLYRPGHYDILYPK 302

BLAST of Cucsat.G16818.T25 vs. ExPASy TrEMBL
Match: A0A1S3BZ50 (Ubiquitin thioesterase OS=Cucumis melo OX=3656 GN=LOC103494633 PE=3 SV=1)

HSP 1 Score: 305 bits (782), Expect = 1.36e-102
Identity = 152/157 (96.82%), Postives = 155/157 (98.73%), Query Frame = 0

Query: 6   YLLFLEQLESALQGNESSISHEELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFI 65
           + LFLEQLESALQGNESSISH+ELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFI
Sbjct: 146 FALFLEQLESALQGNESSISHDELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFI 205

Query: 66  MGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSVNHHD 125
           MGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSVNHHD
Sbjct: 206 MGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSVNHHD 265

Query: 126 FVPATTEVASGSAASEIKIPFITLLYRPGHYDILYPK 162
           FVPATTEV+S SAASEIKIPFITLLYRPGHYDILYPK
Sbjct: 266 FVPATTEVSSDSAASEIKIPFITLLYRPGHYDILYPK 302

BLAST of Cucsat.G16818.T25 vs. ExPASy TrEMBL
Match: A0A5A7TN16 (Ubiquitinyl hydrolase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G002440 PE=4 SV=1)

HSP 1 Score: 305 bits (782), Expect = 2.81e-102
Identity = 152/157 (96.82%), Postives = 155/157 (98.73%), Query Frame = 0

Query: 6   YLLFLEQLESALQGNESSISHEELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFI 65
           + LFLEQLESALQGNESSISH+ELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFI
Sbjct: 142 FALFLEQLESALQGNESSISHDELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFI 201

Query: 66  MGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSVNHHD 125
           MGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSVNHHD
Sbjct: 202 MGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSVNHHD 261

Query: 126 FVPATTEVASGSAASEIKIPFITLLYRPGHYDILYPK 162
           FVPATTEV+S SAASEIKIPFITLLYRPGHYDILYPK
Sbjct: 262 FVPATTEVSSDSAASEIKIPFITLLYRPGHYDILYPK 298

BLAST of Cucsat.G16818.T25 vs. ExPASy TrEMBL
Match: A0A5D3E124 (Ubiquitinyl hydrolase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G002440 PE=4 SV=1)

HSP 1 Score: 305 bits (782), Expect = 2.81e-102
Identity = 152/157 (96.82%), Postives = 155/157 (98.73%), Query Frame = 0

Query: 6   YLLFLEQLESALQGNESSISHEELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFI 65
           + LFLEQLESALQGNESSISH+ELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFI
Sbjct: 142 FALFLEQLESALQGNESSISHDELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFI 201

Query: 66  MGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSVNHHD 125
           MGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSVNHHD
Sbjct: 202 MGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSVNHHD 261

Query: 126 FVPATTEVASGSAASEIKIPFITLLYRPGHYDILYPK 162
           FVPATTEV+S SAASEIKIPFITLLYRPGHYDILYPK
Sbjct: 262 FVPATTEVSSDSAASEIKIPFITLLYRPGHYDILYPK 298

BLAST of Cucsat.G16818.T25 vs. ExPASy TrEMBL
Match: A0A6J1CVP6 (Ubiquitinyl hydrolase 1 OS=Momordica charantia OX=3673 GN=LOC111015206 PE=4 SV=1)

HSP 1 Score: 302 bits (773), Expect = 8.04e-102
Identity = 147/162 (90.74%), Postives = 158/162 (97.53%), Query Frame = 0

Query: 1   SFMFSYLLFLEQLESALQGNESSISHEELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEF 60
           SFMFSYLLFLEQLESALQGNE+SISH+ELV+RSRDQSISDYVVMFFRFV+SGEIQKRSEF
Sbjct: 100 SFMFSYLLFLEQLESALQGNETSISHDELVLRSRDQSISDYVVMFFRFVSSGEIQKRSEF 159

Query: 61  FEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLS 120
           FEPFIMGLTNGTVDQFCK+AVEPMGEESDHVHIIALSDALGVPIRV+YLDRSSCDSGG+S
Sbjct: 160 FEPFIMGLTNGTVDQFCKSAVEPMGEESDHVHIIALSDALGVPIRVIYLDRSSCDSGGVS 219

Query: 121 VNHHDFVPATTEVASGSAASEIKIPFITLLYRPGHYDILYPK 162
           VNHHDFVPA ++V+S  A SEIKIP+ITLLYRPGHYDILYPK
Sbjct: 220 VNHHDFVPAASDVSSDGAGSEIKIPYITLLYRPGHYDILYPK 261

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LG983.6e-6171.97OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 1 OS=Arabidopsis thalian... [more]
Q96FW11.0e-2347.14Ubiquitin thioesterase OTUB1 OS=Homo sapiens OX=9606 GN=OTUB1 PE=1 SV=2[more]
Q7TQI31.0e-2347.14Ubiquitin thioesterase OTUB1 OS=Mus musculus OX=10090 GN=Otub1 PE=1 SV=2[more]
B2RYG61.0e-2347.14Ubiquitin thioesterase OTUB1 OS=Rattus norvegicus OX=10116 GN=Otub1 PE=1 SV=1[more]
Q9VL005.6e-2240.63Ubiquitin thioesterase otubain-like OS=Drosophila melanogaster OX=7227 GN=CG4968... [more]
Match NameE-valueIdentityDescription
XP_004152159.12.97e-10498.73OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 1 [Cucumis sativus] >XP_... [more]
XP_008454143.12.81e-10296.82PREDICTED: ubiquitin thioesterase otubain-like [Cucumis melo] >XP_008454144.1 PR... [more]
TYK29632.15.81e-10296.82ubiquitin thioesterase otubain-like [Cucumis melo var. makuwa][more]
KAA0044504.15.81e-10296.82ubiquitin thioesterase otubain-like [Cucumis melo var. makuwa][more]
XP_022145855.11.66e-10190.74ubiquitin thioesterase otubain-like isoform X2 [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A0A0KX371.44e-10498.73Ubiquitin thioesterase OS=Cucumis sativus OX=3659 GN=Csa_4G007720 PE=3 SV=1[more]
A0A1S3BZ501.36e-10296.82Ubiquitin thioesterase OS=Cucumis melo OX=3656 GN=LOC103494633 PE=3 SV=1[more]
A0A5A7TN162.81e-10296.82Ubiquitinyl hydrolase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A5D3E1242.81e-10296.82Ubiquitinyl hydrolase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A6J1CVP68.04e-10290.74Ubiquitinyl hydrolase 1 OS=Momordica charantia OX=3673 GN=LOC111015206 PE=4 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR042468Peptidase C65, otubain, subdomain 1GENE3D3.30.200.60Peptidase C65 Otubain, subdomain 1coord: 66..162
e-value: 3.6E-28
score: 99.5
IPR019400Peptidase C65, otubainPFAMPF10275Peptidase_C65coord: 9..161
e-value: 1.7E-47
score: 162.0
IPR019400Peptidase C65, otubainPANTHERPTHR12931UBIQUITIN THIOLESTERASE PROTEIN OTUBcoord: 8..162
NoneNo IPR availablePANTHERPTHR12931:SF30UBIQUITIN THIOESTERASEcoord: 8..162
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 5..161

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G16818Cucsat.G16818gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G16818.T25.E1Cucsat.G16818.T25.E1exon
Cucsat.G16818.T25.E2Cucsat.G16818.T25.E2exon
Cucsat.G16818.T25.E3Cucsat.G16818.T25.E3exon
Cucsat.G16818.T25.E4Cucsat.G16818.T25.E4exon
Cucsat.G16818.T25.E5Cucsat.G16818.T25.E5exon
Cucsat.G16818.T25.E6Cucsat.G16818.T25.E6exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G16818.T25.C5Cucsat.G16818.T25.C5CDS
Cucsat.G16818.T25.C4Cucsat.G16818.T25.C4CDS
Cucsat.G16818.T25.C3Cucsat.G16818.T25.C3CDS
Cucsat.G16818.T25.C2Cucsat.G16818.T25.C2CDS
Cucsat.G16818.T25.C1Cucsat.G16818.T25.C1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G16818.T25Cucsat.G16818.T25-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071108 protein K48-linked deubiquitination
cellular_component GO:0005634 nucleus
molecular_function GO:0061815 deubiquitinase, acting on linear ubiquitin
molecular_function GO:1990380 Lys48-specific deubiquitinase activity
molecular_function GO:0019784 NEDD8-specific protease activity
molecular_function GO:0004843 thiol-dependent deubiquitinase
molecular_function GO:0043130 ubiquitin binding