Cucsat.G16350.T4 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G16350.T4
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Protein kinase domain-containing protein
Location	ctg2279: 18356 .. 21929 (+)
RNA-Seq Expression	Cucsat.G16350.T4
Synteny	Cucsat.G16350.T4

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTCACATTGTGTCAAACTAAAAAATAAACAAGAATTTAACTCAATTGTAACAACAAGTTTGACGGTGTAATTGGGAAAACTTGAAGAGAAGAGGAGAAATCAAGGATGTGATATTGTGAAAGGGACGGGAGAGTAAGAGTAAAAGGGGGGAAGTTAGTGGCGTGACTGAACCGTTTGCCACCACCTCTCAAAGAGACGCTTTTGAAATTTCAACGCAGATCTTTTTTTGTTTTGATTTTGGAAAAAGGATGGCCATGGAGCATCAAATCAAAAATGGAGGTAGTTTTCAATTTTCATGGTGGCCCATCTTCTTGGATTTTTGAATTATCATTATCTAACACACATGGAAACATGGAGTTTCTTTAATTTCTAGCTTTGAGTTATTCAAACGTTGAACTCTTTCGTTGACTGTTGAAGCAAAGCTGGATGAGAGAGATGTTGAAGATTGAGGAACACTCTCTTCTTCTTCCTTATATCTTTTTATTCTGTCTTTTGACCTTCCCCTTTTCCCCCCCAATTTTTTTCCCATCTCATTCTTCCTCCCTGTCCTTGTTATCATCATGGGAATCTGCTGCGCTAAACCTGCTAAGCTTGCTCATGCTTCTTCCAGCATTATTTCTGGTGAACTCTCTCTTCAGCTCTTCCGTTTTTTTGTACTTTTGTCCGCCATCGGTAGATTATTGTCAATTTTCATAGCAATGCGATTGGAAAGGAAGCAATTCTCTTGTCAATTATCGGATCTGCATGCTGTTGTAATTTGGTTCTCTTATAAATTTCTAAAGTCTGTTGCTGTTAAGTTACTTGCTGGATGCGCTAACATTTCTATTTATATTGTTCTAGCTTATATTGGGTATTGTGTTATTTGGATGTGAGTTTATTGTCTTTTTTATGTTGGGATTTCCTTAACTGGATCATTTTTCATGGCAGGAACGGATAACAAAAATAGTAAGTCCAACGTGGAATCGATCAATTGTAATTCGAAAACTGTAAATTTTACCTCGAGTGCGCCTGTCATCAAACCGAAATTCGATGTGTCCGGTGCCTCTGCTCTCAAATCTTTTAGCTTCATTGATCTCAAGAATGCTACCAAGAACTTCCGTAGTGAAAGTTTACTTGGTGAGGGAGGATTTGGATGCGTGTTTAAAGGATGGATCGATGAGCACTCTTATCTTCCTACTAAACCTGGAACTGGAATTGTAGTGGCTGTCAAGAAACTCAAGCGAGAGAGCCTTCAAGGCTACAAAGAATGGCTTGTACGTAAACTATGAATTCTCTTATTAATCATAATATGTTTTCCAGATAACTCACCTTAACTTCGTTTGAATCACATCATTTCAGGCAGAAGTAAATTATCTGGGCCAGCTTCGCCATGAAAATCTCGTCAGGCTCATTGGTTATTGCTCTGAATCTGATAACAGACTTCTAGTTTATGAGTATATGCCAAAGGGAAGTCTGGAAAATCACTTGTTTAGAAGTTAGTATGCAACCCCATCACCACCTCTTCAACAACTTTCTTCTCTTGCTAATTTTTAAAGCTGTTGCCTTCTATTCATATTTAGATTAGTGTTTCCCTCCTCCAATCTATAGCACTTCAAGTCTAGCTATTAAAAATTAAGAATTGTCTAAGAAATGTATTATAGGGAACTTTTGTCATTGAAGCATTGTTTATACACAGCATGACTGAAGGAAGAAATCTTTTTGTTGATCCCCCGGCGATCAAGGAGCTAGGCTTTCTATAGTGGCACTTAGGCTGTTAATATCTAACAAACTTCTAGTATCCCTTTAATCAATTACCACAGTTAGTTCTATGGTACAATTATTGCCATCCGGAATAAACTAAACCCTTGCATCAATTCCTTTAAATAGTTCATTTATTTATTTTTGAGTGTGGTGATGCAGGAGTGTGGGCGGGGCAAGTGGCTCTAAAACTTTTATGCTGAGGCTTCTGTTATAAGACGGACGAAACTATGTTGTTTCAATGAAATTGAGCTAGCCAAAAGCAAATGAGAGTAATTCCATGTCCATAGGATAATGAATTGTGTTAAGCCCGACGTAGGTTCACTTGTCAGGGAGTCAGAATAATGTTTGTCATTGCCTTTAGTTGCCACCTTGTTCCTCTTGACTGTGTTTTATTGCTATGAAGTACAACCAGTTATACATGTTTTTCAAATTTTTGACAGGTTTCATGTGTTGTTTCGGTATTTTTATCTGGGAATTCGTAATAATACTCACCTTTCTCTGCTTTTACAGAAGGTGTTACCCCAATTTCTTGGCGTGTTCGAATGGATATCGCAGTTGACGTGGCACGGGGATTGGCATTCTTACACAGTTCCGAACCTAATGTGATATATCGTGATTTGAAGGCTTCCAACATTCTGCTTGACTCAGTGAGTGATTTTTTTTTTCCCTTGTCAATGGAAAGAAAATCCCTTCTGAAGATACTCATTTCTCTCTCTGTTTTTCCTTTGAAGGAATTCAATGCGAAACTATCAGATTTTGGCTTAGCAAGAGAAGGGCCTACGGGAGATAAGACTCATGTTTCTACCCGGGTGATGGGAACTCGAGGCTATGCCGCCCCTGAATATGTAGCAACAGGTTGGCAGAATTCATTTTTTTCATCCTTTAATGTTTCACAAATTGATGAAGTAAAACCAAATATTTAATTTCAGAAAGTACCAAATCTATTTAGTTCTTAGCTCATGTATGGAATGGTCGAACTTATTTTCTTGGTAAAGATTATTAAACTAAAAATTAAGAGGCAGAAAAGAAACGAAGATCCAAGATGAGAGAAATAGTTTTGTACTACATTATGCCCAAATATACTTTTATTCGTTCTTTCCTTCTATGTAGAACAAAGATATTTTTATTCAGGAAATGATGCATTAATACAAGGGTACTGCACTCGTCTCTGACTTTGCTTGGTGATGGTTTGGCCAACTAAAACAGGTCACTTAACTCCGAAGAGCGATGTATACAGCTTTGGTGTTGTTTTACTAGAACTTCTCTCAGGCAAGCGTGCACTAGACCACGAGAAAGTTGGGAGAGTTGAAGAGACCCTTGTAGATTGGGGCAAACCACTCCTAAGCGATGGTAAGCGAATGTTGAGGATTATGGATACAAGGATGGGCGGTCAATATTCGAGAAAAGAAGCTCAAGCTGCAGCTTCGCTTGCACTGAATTGTCTCCATACAGATCCAAAAAACAGGCCATCTATGGCCGAAGTTCTTGACGAATTGGAACGACTACACACTGCAAAGGATATTCTGGGGACCCCAAATGCTCATGCCATCAGACGAACTCCACCTTCTAGATTTCAATCATAATTTGTTTCGAATTGTTTCACCTGCCAACAAACGCACGTCTTACCTACTACACAAGATGTTCGATGTACAAGTTTTGTGGATATTTCAAGTAGAATATACACCCTAGAAAATGTAATCAACACTTACATTTATATATAGACCTTTCAAGTCTCCATATGAGAAATTACGAAATGGCATATGATAAAAAATGTGCACAATCAATTGAAGTAAACAAGATCATTCAGAACATGCCCTAATAAACAAGACTACT

Coding sequence (CDS)

ATGGATATCGCAGTTGACGTGGCACGGGGATTGGCATTCTTACACAGTTCCGAACCTAATGTGATATATCGTGATTTGAAGGCTTCCAACATTCTGCTTGACTCAGTGAGTGATTTTTTTTTTCCCTTGTCAATGGAAAGAAAATCCCTTCTGAAGATACTCATTTCTCTCTCTGTTTTTCCTTTGAAGGAATTCAATGCGAAACTATCAGATTTTGGCTTAGCAAGAGAAGGGCCTACGGGAGATAAGACTCATGTTTCTACCCGGGTGATGGGAACTCGAGGCTATGCCGCCCCTGAATATGTAGCAACAGGTCACTTAACTCCGAAGAGCGATGTATACAGCTTTGGTGTTGTTTTACTAGAACTTCTCTCAGGCAAGCGTGCACTAGACCACGAGAAAGTTGGGAGAGTTGAAGAGACCCTTGTAGATTGGGGCAAACCACTCCTAAGCGATGGTAAGCGAATGTTGAGGATTATGGATACAAGGATGGGCGGTCAATATTCGAGAAAAGAAGCTCAAGCTGCAGCTTCGCTTGCACTGAATTGTCTCCATACAGATCCAAAAAACAGGCCATCTATGGCCGAAGTTCTTGACGAATTGGAACGACTACACACTGCAAAGGATATTCTGGGGACCCCAAATGCTCATGCCATCAGACGAACTCCACCTTCTAGATTTCAATCATAA

Protein sequence

MDIAVDVARGLAFLHSSEPNVIYRDLKASNILLDSVSDFFFPLSMERKSLLKILISLSVFPLKEFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDHEKVGRVEETLVDWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLALNCLHTDPKNRPSMAEVLDELERLHTAKDILGTPNAHAIRRTPPSRFQS

Homology

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BLAST of Cucsat.G16350.T4 vs. ExPASy Swiss-Prot
Match: O49840 (Probable serine/threonine-protein kinase PBL3 OS=Arabidopsis thaliana OX=3702 GN=PBL3 PE=1 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.1e-60
Identity = 124/218 (56.88%), Postives = 150/218 (68.81%), Query Frame = 0


Query: 1   MDIAVDVARGLAFLHSSEPNVIYRDLKASNILLDSVSDFFFPLSMERKSLLKILISLSVF 60
           M +A+  A+GL FLH ++  VIYRD KA+NILLD+                         
Sbjct: 192 MKVAIGAAKGLTFLHDAKSQVIYRDFKAANILLDA------------------------- 251

Query: 61  PLKEFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL 120
              EFN+KLSDFGLA+ GPTGDKTHVST+VMGT GYAAPEYVATG LT KSDVYSFGVVL
Sbjct: 252 ---EFNSKLSDFGLAKAGPTGDKTHVSTQVMGTHGYAAPEYVATGRLTAKSDVYSFGVVL 311

Query: 121 LELLSGKRALDHEKVGRVEETLVDWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLA 180
           LELLSG+RA+D  KVG +E++LVDW  P L D +++ RIMDTR+GGQY +K A  AASLA
Sbjct: 312 LELLSGRRAVDKSKVG-MEQSLVDWATPYLGDKRKLFRIMDTRLGGQYPQKGAYTAASLA 371

Query: 181 LNCLHTDPKNRPSMAEVLDELERLHTAKDILGTPNAHA 219
           L CL+ D K RP M+EVL +L++L + K   G  N  A
Sbjct: 372 LQCLNPDAKLRPKMSEVLAKLDQLESTKPGTGVGNRQA 380

[Close]

BLAST of Cucsat.G16350.T4 vs. ExPASy Swiss-Prot
Match: Q5XF79 (Probable serine/threonine-protein kinase PBL18 OS=Arabidopsis thaliana OX=3702 GN=PBL18 PE=2 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 4.5e-57
Identity = 120/213 (56.34%), Postives = 146/213 (68.54%), Query Frame = 0


Query: 1   MDIAVDVARGLAFLHSSEPNVIYRDLKASNILLDSVSDFFFPLSMERKSLLKILISLSVF 60
           M +A   ARGL+FLH  E  VIYRD KASNILLD                          
Sbjct: 193 MKVAFSAARGLSFLH--EAKVIYRDFKASNILLD-------------------------- 252

Query: 61  PLKEFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL 120
              +FNAKLSDFGLA+ GPTGD+THV+T+V+GT+GYAAPEY+ATG LT KSDVYSFGVVL
Sbjct: 253 --VDFNAKLSDFGLAKAGPTGDRTHVTTQVIGTQGYAAPEYIATGRLTSKSDVYSFGVVL 312

Query: 121 LELLSGKRALDHEKVGRVEETLVDWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLA 180
           LELLSG+  LD  KVG VE  LVDW  P L D +++ RIMDT++GGQY  K A AAA++A
Sbjct: 313 LELLSGRPTLDKSKVG-VERNLVDWAIPYLVDRRKVFRIMDTKLGGQYPHKGACAAANIA 372

Query: 181 LNCLHTDPKNRPSMAEVLDELERLHTAKDILGT 214
           L CL+T+PK RP MA+VL  L++L T+   +G+
Sbjct: 373 LRCLNTEPKLRPDMADVLSTLQQLETSSKKMGS 374

[Close]

BLAST of Cucsat.G16350.T4 vs. ExPASy Swiss-Prot
Match: P43293 (Probable serine/threonine-protein kinase PBL11 OS=Arabidopsis thaliana OX=3702 GN=PBL11 PE=1 SV=2)

HSP 1 Score: 218.0 bits (554), Expect = 1.1e-55
Identity = 115/208 (55.29%), Postives = 145/208 (69.71%), Query Frame = 0


Query: 3   IAVDVARGLAFLHSSEPNVIYRDLKASNILLDSVSDFFFPLSMERKSLLKILISLSVFPL 62
           +A+  ARGLAFLH+++P VIYRD KASNILLDS                           
Sbjct: 181 MALGAARGLAFLHNAQPQVIYRDFKASNILLDS--------------------------- 240

Query: 63  KEFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLE 122
             +NAKLSDFGLAR+GP GD +HVSTRVMGT+GYAAPEY+ATGHL+ KSDVYSFGVVLLE
Sbjct: 241 -NYNAKLSDFGLARDGPMGDNSHVSTRVMGTQGYAAPEYLATGHLSVKSDVYSFGVVLLE 300

Query: 123 LLSGKRALD-HEKVGRVEETLVDWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLAL 182
           LLSG+RA+D ++ VG  E  LVDW +P L++ +R+LR+MD R+ GQYS   A   A LAL
Sbjct: 301 LLSGRRAIDKNQPVG--EHNLVDWARPYLTNKRRLLRVMDPRLQGQYSLTRALKIAVLAL 358

Query: 183 NCLHTDPKNRPSMAEVLDELERLHTAKD 210
           +C+  D K+RP+M E++  +E LH  K+
Sbjct: 361 DCISIDAKSRPTMNEIVKTMEELHIQKE 358

[Close]

BLAST of Cucsat.G16350.T4 vs. ExPASy Swiss-Prot
Match: O49839 (Probable serine/threonine-protein kinase PBL2 OS=Arabidopsis thaliana OX=3702 GN=PBL2 PE=1 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 1.4e-55
Identity = 115/206 (55.83%), Postives = 141/206 (68.45%), Query Frame = 0


Query: 1   MDIAVDVARGLAFLHSSEPNVIYRDLKASNILLDSVSDFFFPLSMERKSLLKILISLSVF 60
           M +AV  A+GL FLH ++  VIYRD KA+NILLD+                         
Sbjct: 195 MKVAVGAAKGLTFLHEAKSQVIYRDFKAANILLDA------------------------- 254

Query: 61  PLKEFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL 120
              +FNAKLSDFGLA+ GPTGD THVST+V+GT GYAAPEYVATG LT KSDVYSFGVVL
Sbjct: 255 ---DFNAKLSDFGLAKAGPTGDNTHVSTKVIGTHGYAAPEYVATGRLTAKSDVYSFGVVL 314

Query: 121 LELLSGKRALDHEKVGRVEETLVDWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLA 180
           LEL+SG+RA+D+   G  E +LVDW  P L D +++ RIMDT++GGQY +K A  AA+LA
Sbjct: 315 LELISGRRAMDNSNGGN-EYSLVDWATPYLGDKRKLFRIMDTKLGGQYPQKGAFTAANLA 371

Query: 181 LNCLHTDPKNRPSMAEVLDELERLHT 207
           L CL+ D K RP M+EVL  LE+L +
Sbjct: 375 LQCLNPDAKLRPKMSEVLVTLEQLES 371

[Close]

BLAST of Cucsat.G16350.T4 vs. ExPASy Swiss-Prot
Match: Q65XV8 (Receptor-like cytoplasmic kinase 176 OS=Oryza sativa subsp. japonica OX=39947 GN=RLCK176 PE=1 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 1.9e-55
Identity = 114/214 (53.27%), Postives = 144/214 (67.29%), Query Frame = 0


Query: 1   MDIAVDVARGLAFLHSSEPNVIYRDLKASNILLDSVSDFFFPLSMERKSLLKILISLSVF 60
           M IA+  A+GLAFLHS +  VIYRD K SN+LLD+                         
Sbjct: 181 MKIALGAAKGLAFLHSDKVKVIYRDFKTSNVLLDA------------------------- 240

Query: 61  PLKEFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL 120
               ++AKLSDFGLA++GPTGDK+HVSTRVMGT GYAAPEY+ATGHLT KSDVYSFGVVL
Sbjct: 241 ---NYDAKLSDFGLAKDGPTGDKSHVSTRVMGTYGYAAPEYLATGHLTTKSDVYSFGVVL 300

Query: 121 LELLSGKRALDHEKVGRVEETLVDWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLA 180
           LE+LSG+RALD  +    E  LV+W +P L   +R+ RI+D R+GGQYS  +AQ AA+LA
Sbjct: 301 LEMLSGRRALDKNR-PTGEHNLVEWARPYLMSKRRIFRILDARLGGQYSLAKAQKAATLA 360

Query: 181 LNCLHTDPKNRPSMAEVLDELERLHTAKDILGTP 215
           L C+  + KNRP+M +V+  LE+L  +K+    P
Sbjct: 361 LQCISVEAKNRPNMEQVVAVLEQLQDSKETGANP 365

[Close]

BLAST of Cucsat.G16350.T4 vs. NCBI nr
Match: XP_011653538.1 (probable serine/threonine-protein kinase PBL3 [Cucumis sativus] >KGN54046.1 hypothetical protein Csa_021645 [Cucumis sativus])

HSP 1 Score: 387 bits (995), Expect = 2.99e-132
Identity = 201/229 (87.77%), Postives = 201/229 (87.77%), Query Frame = 0


Query: 1   MDIAVDVARGLAFLHSSEPNVIYRDLKASNILLDSVSDFFFPLSMERKSLLKILISLSVF 60
           MDIAVDVARGLAFLHSSEPNVIYRDLKASNILLDS                         
Sbjct: 186 MDIAVDVARGLAFLHSSEPNVIYRDLKASNILLDS------------------------- 245

Query: 61  PLKEFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL 120
              EFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL
Sbjct: 246 ---EFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL 305

Query: 121 LELLSGKRALDHEKVGRVEETLVDWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLA 180
           LELLSGKRALDHEKVGRVEETLVDWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLA
Sbjct: 306 LELLSGKRALDHEKVGRVEETLVDWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLA 365

Query: 181 LNCLHTDPKNRPSMAEVLDELERLHTAKDILGTPNAHAIRRTPPSRFQS 229
           LNCLHTDPKNRPSMAEVLDELERLHTAKDILGTPNAHAIRRTPPSRFQS
Sbjct: 366 LNCLHTDPKNRPSMAEVLDELERLHTAKDILGTPNAHAIRRTPPSRFQS 386

[Close]

BLAST of Cucsat.G16350.T4 vs. NCBI nr
Match: XP_008449857.1 (PREDICTED: protein kinase 2B, chloroplastic-like [Cucumis melo] >KAA0040087.1 protein kinase 2B [Cucumis melo var. makuwa] >TYK21713.1 protein kinase 2B [Cucumis melo var. makuwa])

HSP 1 Score: 370 bits (951), Expect = 1.30e-125
Identity = 192/224 (85.71%), Postives = 193/224 (86.16%), Query Frame = 0


Query: 1   MDIAVDVARGLAFLHSSEPNVIYRDLKASNILLDSVSDFFFPLSMERKSLLKILISLSVF 60
           MDIAVDVARGLAFLHSS PNVIYRDLKASNILLDS                         
Sbjct: 186 MDIAVDVARGLAFLHSSRPNVIYRDLKASNILLDS------------------------- 245

Query: 61  PLKEFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL 120
              EFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL
Sbjct: 246 ---EFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL 305

Query: 121 LELLSGKRALDHEKVGRVEETLVDWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLA 180
           LELLSGKRALD EKVGRVEETLVDWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLA
Sbjct: 306 LELLSGKRALDQEKVGRVEETLVDWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLA 365

Query: 181 LNCLHTDPKNRPSMAEVLDELERLHTAKDILGTPNAHAIRRTPP 224
           LNCLHTDPKNRPSMAEVLDELERLH AKD+LGTPNAHAIRRTPP
Sbjct: 366 LNCLHTDPKNRPSMAEVLDELERLHAAKDVLGTPNAHAIRRTPP 381

[Close]

BLAST of Cucsat.G16350.T4 vs. NCBI nr
Match: XP_038900470.1 (probable serine/threonine-protein kinase PBL3 [Benincasa hispida])

HSP 1 Score: 363 bits (931), Expect = 1.57e-122
Identity = 187/229 (81.66%), Postives = 195/229 (85.15%), Query Frame = 0


Query: 1   MDIAVDVARGLAFLHSSEPNVIYRDLKASNILLDSVSDFFFPLSMERKSLLKILISLSVF 60
           M+IAVDVARGLAFLHSSEPNVIYRDLKA+NILLDS                         
Sbjct: 186 MNIAVDVARGLAFLHSSEPNVIYRDLKAANILLDS------------------------- 245

Query: 61  PLKEFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL 120
              EFNAKLSDFGLAREGPTGDKTHVSTRV+GTRGYAAPEYVATGHLTPKSDVYSFGVVL
Sbjct: 246 ---EFNAKLSDFGLAREGPTGDKTHVSTRVIGTRGYAAPEYVATGHLTPKSDVYSFGVVL 305

Query: 121 LELLSGKRALDHEKVGRVEETLVDWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLA 180
           LELLSGKRALD EKVGRVEE+LVDWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLA
Sbjct: 306 LELLSGKRALDQEKVGRVEESLVDWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLA 365

Query: 181 LNCLHTDPKNRPSMAEVLDELERLHTAKDILGTPNAHAIRRTPPSRFQS 229
           LNCLHTDPKNRPSM+EVLDELERLH  KD+  TP+ HAIRRTPP+RFQS
Sbjct: 366 LNCLHTDPKNRPSMSEVLDELERLHATKDVPRTPDVHAIRRTPPARFQS 386

[Close]

BLAST of Cucsat.G16350.T4 vs. NCBI nr
Match: XP_022957668.1 (probable serine/threonine-protein kinase PBL3 isoform X2 [Cucurbita moschata])

HSP 1 Score: 349 bits (895), Expect = 2.52e-117
Identity = 181/226 (80.09%), Postives = 191/226 (84.51%), Query Frame = 0


Query: 1   MDIAVDVARGLAFLHSSEPNVIYRDLKASNILLDSVSDFFFPLSMERKSLLKILISLSVF 60
           M+IAVDVARGL+FLHS EPNVIYRDLKASNILLDS                         
Sbjct: 169 MNIAVDVARGLSFLHSCEPNVIYRDLKASNILLDS------------------------- 228

Query: 61  PLKEFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL 120
              EFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL
Sbjct: 229 ---EFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL 288

Query: 121 LELLSGKRALDHEKVGRVEETLVDWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLA 180
           LELLSGKRALD EKVGRVEETLVDWGKPLLSD KRMLRIMDTRMGGQYSRKEAQAAASLA
Sbjct: 289 LELLSGKRALDQEKVGRVEETLVDWGKPLLSDSKRMLRIMDTRMGGQYSRKEAQAAASLA 348

Query: 181 LNCLHTDPKNRPSMAEVLDELERLHTAKDILGTPNAHAIRRTPPSR 226
           LNCLHTDPKNRPSM +VL+ELERL ++KD+L TP++ AIRRTPP+R
Sbjct: 349 LNCLHTDPKNRPSMVDVLEELERLLSSKDVLRTPDSRAIRRTPPAR 366

[Close]

BLAST of Cucsat.G16350.T4 vs. NCBI nr
Match: XP_023533196.1 (probable serine/threonine-protein kinase PBL3 isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 349 bits (895), Expect = 2.52e-117
Identity = 181/226 (80.09%), Postives = 191/226 (84.51%), Query Frame = 0


Query: 1   MDIAVDVARGLAFLHSSEPNVIYRDLKASNILLDSVSDFFFPLSMERKSLLKILISLSVF 60
           M+IAVDVARGL+FLHS EPNVIYRDLKASNILLDS                         
Sbjct: 169 MNIAVDVARGLSFLHSCEPNVIYRDLKASNILLDS------------------------- 228

Query: 61  PLKEFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL 120
              EFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL
Sbjct: 229 ---EFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL 288

Query: 121 LELLSGKRALDHEKVGRVEETLVDWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLA 180
           LELLSGKRALD EKVGRVEETLVDWGKPLLSD KRMLRIMDTRMGGQYSRKEAQAAASLA
Sbjct: 289 LELLSGKRALDQEKVGRVEETLVDWGKPLLSDSKRMLRIMDTRMGGQYSRKEAQAAASLA 348

Query: 181 LNCLHTDPKNRPSMAEVLDELERLHTAKDILGTPNAHAIRRTPPSR 226
           LNCLHTDPKNRPSM +VL+ELERL ++KD+L TP++ AIRRTPP+R
Sbjct: 349 LNCLHTDPKNRPSMVDVLEELERLLSSKDVLRTPDSRAIRRTPPAR 366

[Close]

BLAST of Cucsat.G16350.T4 vs. ExPASy TrEMBL
Match: A0A0A0L1P7 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G269770 PE=3 SV=1)

HSP 1 Score: 387 bits (995), Expect = 1.45e-132
Identity = 201/229 (87.77%), Postives = 201/229 (87.77%), Query Frame = 0


Query: 1   MDIAVDVARGLAFLHSSEPNVIYRDLKASNILLDSVSDFFFPLSMERKSLLKILISLSVF 60
           MDIAVDVARGLAFLHSSEPNVIYRDLKASNILLDS                         
Sbjct: 186 MDIAVDVARGLAFLHSSEPNVIYRDLKASNILLDS------------------------- 245

Query: 61  PLKEFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL 120
              EFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL
Sbjct: 246 ---EFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL 305

Query: 121 LELLSGKRALDHEKVGRVEETLVDWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLA 180
           LELLSGKRALDHEKVGRVEETLVDWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLA
Sbjct: 306 LELLSGKRALDHEKVGRVEETLVDWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLA 365

Query: 181 LNCLHTDPKNRPSMAEVLDELERLHTAKDILGTPNAHAIRRTPPSRFQS 229
           LNCLHTDPKNRPSMAEVLDELERLHTAKDILGTPNAHAIRRTPPSRFQS
Sbjct: 366 LNCLHTDPKNRPSMAEVLDELERLHTAKDILGTPNAHAIRRTPPSRFQS 386

[Close]

BLAST of Cucsat.G16350.T4 vs. ExPASy TrEMBL
Match: A0A5A7TEX2 (Protein kinase 2B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00820 PE=3 SV=1)

HSP 1 Score: 370 bits (951), Expect = 6.31e-126
Identity = 192/224 (85.71%), Postives = 193/224 (86.16%), Query Frame = 0


Query: 1   MDIAVDVARGLAFLHSSEPNVIYRDLKASNILLDSVSDFFFPLSMERKSLLKILISLSVF 60
           MDIAVDVARGLAFLHSS PNVIYRDLKASNILLDS                         
Sbjct: 186 MDIAVDVARGLAFLHSSRPNVIYRDLKASNILLDS------------------------- 245

Query: 61  PLKEFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL 120
              EFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL
Sbjct: 246 ---EFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL 305

Query: 121 LELLSGKRALDHEKVGRVEETLVDWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLA 180
           LELLSGKRALD EKVGRVEETLVDWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLA
Sbjct: 306 LELLSGKRALDQEKVGRVEETLVDWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLA 365

Query: 181 LNCLHTDPKNRPSMAEVLDELERLHTAKDILGTPNAHAIRRTPP 224
           LNCLHTDPKNRPSMAEVLDELERLH AKD+LGTPNAHAIRRTPP
Sbjct: 366 LNCLHTDPKNRPSMAEVLDELERLHAAKDVLGTPNAHAIRRTPP 381

[Close]

BLAST of Cucsat.G16350.T4 vs. ExPASy TrEMBL
Match: A0A1S3BNM8 (protein kinase 2B, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103491609 PE=3 SV=1)

HSP 1 Score: 370 bits (951), Expect = 6.31e-126
Identity = 192/224 (85.71%), Postives = 193/224 (86.16%), Query Frame = 0


Query: 1   MDIAVDVARGLAFLHSSEPNVIYRDLKASNILLDSVSDFFFPLSMERKSLLKILISLSVF 60
           MDIAVDVARGLAFLHSS PNVIYRDLKASNILLDS                         
Sbjct: 186 MDIAVDVARGLAFLHSSRPNVIYRDLKASNILLDS------------------------- 245

Query: 61  PLKEFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL 120
              EFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL
Sbjct: 246 ---EFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL 305

Query: 121 LELLSGKRALDHEKVGRVEETLVDWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLA 180
           LELLSGKRALD EKVGRVEETLVDWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLA
Sbjct: 306 LELLSGKRALDQEKVGRVEETLVDWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLA 365

Query: 181 LNCLHTDPKNRPSMAEVLDELERLHTAKDILGTPNAHAIRRTPP 224
           LNCLHTDPKNRPSMAEVLDELERLH AKD+LGTPNAHAIRRTPP
Sbjct: 366 LNCLHTDPKNRPSMAEVLDELERLHAAKDVLGTPNAHAIRRTPP 381

[Close]

BLAST of Cucsat.G16350.T4 vs. ExPASy TrEMBL
Match: A0A6J1H0W4 (probable serine/threonine-protein kinase PBL3 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111459133 PE=3 SV=1)

HSP 1 Score: 349 bits (895), Expect = 1.22e-117
Identity = 181/226 (80.09%), Postives = 191/226 (84.51%), Query Frame = 0


Query: 1   MDIAVDVARGLAFLHSSEPNVIYRDLKASNILLDSVSDFFFPLSMERKSLLKILISLSVF 60
           M+IAVDVARGL+FLHS EPNVIYRDLKASNILLDS                         
Sbjct: 169 MNIAVDVARGLSFLHSCEPNVIYRDLKASNILLDS------------------------- 228

Query: 61  PLKEFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL 120
              EFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL
Sbjct: 229 ---EFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL 288

Query: 121 LELLSGKRALDHEKVGRVEETLVDWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLA 180
           LELLSGKRALD EKVGRVEETLVDWGKPLLSD KRMLRIMDTRMGGQYSRKEAQAAASLA
Sbjct: 289 LELLSGKRALDQEKVGRVEETLVDWGKPLLSDSKRMLRIMDTRMGGQYSRKEAQAAASLA 348

Query: 181 LNCLHTDPKNRPSMAEVLDELERLHTAKDILGTPNAHAIRRTPPSR 226
           LNCLHTDPKNRPSM +VL+ELERL ++KD+L TP++ AIRRTPP+R
Sbjct: 349 LNCLHTDPKNRPSMVDVLEELERLLSSKDVLRTPDSRAIRRTPPAR 366

[Close]

BLAST of Cucsat.G16350.T4 vs. ExPASy TrEMBL
Match: A0A6J1GZR7 (probable serine/threonine-protein kinase PBL3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459133 PE=3 SV=1)

HSP 1 Score: 349 bits (895), Expect = 2.13e-117
Identity = 181/226 (80.09%), Postives = 191/226 (84.51%), Query Frame = 0


Query: 1   MDIAVDVARGLAFLHSSEPNVIYRDLKASNILLDSVSDFFFPLSMERKSLLKILISLSVF 60
           M+IAVDVARGL+FLHS EPNVIYRDLKASNILLDS                         
Sbjct: 186 MNIAVDVARGLSFLHSCEPNVIYRDLKASNILLDS------------------------- 245

Query: 61  PLKEFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL 120
              EFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL
Sbjct: 246 ---EFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVL 305

Query: 121 LELLSGKRALDHEKVGRVEETLVDWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLA 180
           LELLSGKRALD EKVGRVEETLVDWGKPLLSD KRMLRIMDTRMGGQYSRKEAQAAASLA
Sbjct: 306 LELLSGKRALDQEKVGRVEETLVDWGKPLLSDSKRMLRIMDTRMGGQYSRKEAQAAASLA 365

Query: 181 LNCLHTDPKNRPSMAEVLDELERLHTAKDILGTPNAHAIRRTPPSR 226
           LNCLHTDPKNRPSM +VL+ELERL ++KD+L TP++ AIRRTPP+R
Sbjct: 366 LNCLHTDPKNRPSMVDVLEELERLLSSKDVLRTPDSRAIRRTPPAR 383

The following BLAST results are available for this feature:

BLAST of Cucsat.G16350.T4 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49840	1.1e-60	56.88	Probable serine/threonine-protein kinase PBL3 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q5XF79	4.5e-57	56.34	Probable serine/threonine-protein kinase PBL18 OS=Arabidopsis thaliana OX=3702 G...	[more]
P43293	1.1e-55	55.29	Probable serine/threonine-protein kinase PBL11 OS=Arabidopsis thaliana OX=3702 G...	[more]
O49839	1.4e-55	55.83	Probable serine/threonine-protein kinase PBL2 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q65XV8	1.9e-55	53.27	Receptor-like cytoplasmic kinase 176 OS=Oryza sativa subsp. japonica OX=39947 GN...	[more]

BLAST of Cucsat.G16350.T4 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011653538.1	2.99e-132	87.77	probable serine/threonine-protein kinase PBL3 [Cucumis sativus] >KGN54046.1 hypo...	[more]
XP_008449857.1	1.30e-125	85.71	PREDICTED: protein kinase 2B, chloroplastic-like [Cucumis melo] >KAA0040087.1 pr...	[more]
XP_038900470.1	1.57e-122	81.66	probable serine/threonine-protein kinase PBL3 [Benincasa hispida]	[more]
XP_022957668.1	2.52e-117	80.09	probable serine/threonine-protein kinase PBL3 isoform X2 [Cucurbita moschata]	[more]
XP_023533196.1	2.52e-117	80.09	probable serine/threonine-protein kinase PBL3 isoform X3 [Cucurbita pepo subsp. ...	[more]

BLAST of Cucsat.G16350.T4 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L1P7	1.45e-132	87.77	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G269...	[more]
A0A5A7TEX2	6.31e-126	85.71	Protein kinase 2B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00...	[more]
A0A1S3BNM8	6.31e-126	85.71	protein kinase 2B, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103491609 PE...	[more]
A0A6J1H0W4	1.22e-117	80.09	probable serine/threonine-protein kinase PBL3 isoform X2 OS=Cucurbita moschata O...	[more]
A0A6J1GZR7	2.13e-117	80.09	probable serine/threonine-protein kinase PBL3 isoform X1 OS=Cucurbita moschata O...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 1..208 e-value: 6.9E-7 score: -37.2
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 3..199 e-value: 7.3E-29 score: 100.9
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 1..204 score: 27.227043
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 1..216 e-value: 6.2E-56 score: 191.0
None	No IPR available	PANTHER	PTHR45621	OS01G0588500 PROTEIN-RELATED	coord: 61..207 coord: 1..35
None	No IPR available	PANTHER	PTHR45621:SF111	OS07G0686800 PROTEIN	coord: 61..207 coord: 1..35
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 21..33
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 2..209

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G16350	Cucsat.G16350	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G16350.T4.E1	Cucsat.G16350.T4.E1	exon
Cucsat.G16350.T4.E2	Cucsat.G16350.T4.E2	exon
Cucsat.G16350.T4.E3	Cucsat.G16350.T4.E3	exon
Cucsat.G16350.T4.E4	Cucsat.G16350.T4.E4	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G16350.T4.C1	Cucsat.G16350.T4.C1	CDS
Cucsat.G16350.T4.C2	Cucsat.G16350.T4.C2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G16350.T4	Cucsat.G16350.T4-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity
molecular_function	GO:0004674	protein serine/threonine kinase activity