Cucsat.G15977.T1 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G15977.T1
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	WRKY domain-containing protein
Location	ctg2178: 781915 .. 785422 (-)
RNA-Seq Expression	Cucsat.G15977.T1
Synteny	Cucsat.G15977.T1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCTTTTATTATAAAAACTCATCCACTCCTTACAACCAGCTGCTTGTTTTCTTTTCACACCAAGTCTATTATTCCCCCAATGGCCAGCTTCCCTTATGAAGATTCAAACCTTAACCCTAACCCTAACCCTAATAACTATACTCATTTTCCTCCGATTCTCGATCCCGCTTCTTTCTTTGATTTTGAGCTCTCTGATTTCCTTTTGTTTGGCGATGACAACAATATCGTCGTCGACCAAGTTGCATCGTCCTCGCCAAGTATGACTTCGTCCGAGAAGATCACCAGTGGAGGAGTTGATAGTGGTGGTAGCTCTACTGTAATTGATACCGGATCTAGCGTAGTAGTTTCTTCGTCGGGAGCGTCAACGACATCTATGCAAGTGCCTTAATTAGCTCTAATTAAATCCAAACCCCTTTTCTCTCAACTTCAATTTGCTTGTTTTTGTAATATGTTGAGAGTTAATTAAAAGAGGTATGTTTTTAATTTTTTCTTCTGTGCCTTTGTGTTTTGCAGAAGAAGCAAAAATGGAGAGAAGAAAAGGAAAGGGGAAATGGGATGTAGAGTTGCATTTAGAACCAAATCAGAGCAAGAAATCATGGATGATGGCTACAAGTGGAGGAAGTATGGGAAAAAATCTGTGAAGAATAGTCCCAATCCCAGGTAATTAATTAAATTAATTCATTATTATACACCTTTAAGTTAAACCCAAAAATAATATTAGGAAGCTAGCTTATGATGTTGCTAAACTTATTAACCTTTCAATCCAAGTATAATGGTTGAACTATGTAACATAGTACTTGTAAAAGTTAATATACCAAAGATATAATTAATTAAACCTAAACTCAATCTAATTAAATGAAATTTACAAATATATCTTAATCCTTGAACTATGTTTATGTATTAGATGAAGTTTAGAATTGATTTGGTTCAGTGCCTTTTTAGTTAATTAGAATAATTGAGTTTATGGTCCATGTTGATTTAGACATGTAAATTTATAGATGTCATATTCAGCTGTCATGGACCATGTTGGAGGCGGCTTGAGTAGTCAAAGCAGTAATCAAGTAAAGTTTTAGATAACATAATATATATATATATATATATATATATATATAATTGCTATGTGATTCACATTAGATTTTGGAGAGAGTAGAATTTTTTAAAAATATGATTGTATAGGAGAATTTATAAACTAATTGATCTATTGGTTCAATTGAGTTTTCGAGTTATTATTGTGTTTTAGTTGGATGTTGAGTTCGAATTGAATTAAAAATGCAGAAAATATATTTTAGAGATGTGAAAAGAAAATATAACAAAAAGATAGTAAGAAAGCATCACTAAATAGAAAGACTTGTTATCAAACAATTATCTTTATTATCTTGAAATAGAAAAAAAAATATTTTAGTCTCGTCATTAAAAAAAAATCAACATGTACTATTTATAGCCAGAAATCTGTTTTCTTTTATTTTAAAAAATTATATGCAATCTATATAAATACTATAAATAAGAGAATGAAAAAATAGAATATTTCAAAAAGAAAATTAATTTTGTATTTTAGTAATTTTTTTTTTATAAACGTAAACAGTTTGTTAATTTTAAAATTTTTAAAAATAATGAATAAATAGAATAACCTCATTGACTAAATTAATTTTTTAAAATATATATTCAAAATTAAAAGAGAGTATTTTAACTTTAGGACTATATTTTAAAGCCAATATCAATAAAAGAAAAATGGATTGAGAGTATTTATAAAAATTACCCTATTTATTAATAATAATAAATAGGTAATCACTTTCTTATTCTTGAGTGTTTTGTTTTTATTTTTATCTTTTGGAGTTTAGTGGTTTCAACTTTTGTTCTTATCGCTTTAGGATATTGTATATGTAATGTATACAAGCATGCTTCATTATTTTATATTTCTCATTCTTTCCCCAATTACTTTAAAAGACACATTGCCATATTTTAAATCAAAATATTTAATTTTAACTTTATTCTTTGTTTTAAAAACTAATTATAAAAACAAAATATTTAATTTTAACTTTATTCTTTGTTCTAAAAACTAATTATAAAAACCATATACAAAACATTTTCAAATATACCATAATTAATGAACTATGTGTTGATAGACACTGAAAGACTTTTATCAATGTCGATCAATGCAGTATTGATATACCTTTATCAATGGTGTATCAATACCTATAGTAGATAGAATATAAATTTTCTTTTAGAAAGGGATACCTTAGATGATCAGTTGACACATTCAAGCATTTTCACTATAATTTGTAAATATATGTTGGTTCATTAACTACGCTATATTTATATCTGAAATATTTATATATTATTTTTATAAATTTATCAGTTTATTAATAGACATTAATAGAAGTCTTTCAGAGTTTATCGCTAATACAATTTAAAATTTTGCTATATTTTGTCAATATTTTCAATGAATTTGTCATTTATATTATTATTTTTTCAAATCATATGCAATAAGCACGAGTATAATAGCTTTTTTTTAGTTAAAAAAAATCAAGAGATTATTAACTTCAAACCCTATGACCTATAAATTCACAATTTCATTCTTGGTCTCAAACACTTCTAATAAGTTATCAAGTCTAATATGGTGTAAAGTCTTTTAACGCACGAGAATTAACTTTAAAATCTAAATATAATATTGTAAATTCGATTTTGAATTAGTTTAAGTAGAAAGAAAGATGATAAACATACAACATATATACATATGGATTAATACAAAAGTATGGTTTTAATTTGGATACAGAAATTACTATAAATGTTCAAGTGAAGGATGCAATGTGAAGAAGAAGGTGGAAAGAGACAGAGAGGATGCAAACTATGTGATTACTACTTATGAGGGAATTCACAACCATGAAAGCCCCTTCGTTGTGTATTACAATCAATTGCCATCCTTTACCTCTGCCTCTACTCCCACCTAGCAAATCCTATTTTCCGGAACTTATATAGTTGTCGGATCAGGTTTAGATTGACTTTCTAAGTATTTAAAAACATTATTTTTTAATCATTTAGTAGGTCAATCCAAACATACCCTAAATTATAATATGTTCATTTTTATTTCCATGTATAGGATCAATTTTGTATTTTGTATAAAATTTACATAGATAAATATAGACGTGAAAGCTTTTTGAAATAATTAGCAACGTTTTATATATAATTTCAATCTATTGACCGATAGACTTCATCGTTGCTATATTTGCAAATTCTTATGAACTACGTTATATTTAGTAATATCTCGAGAATTGCCACATGATTGCAATTGTCCCATAATTAAAGTCATTTATTTTCTTAACCGTTGTATCCTTGTTTAGTTCAAAATAAAATCAAAAGGGCACCCAATGTTTTTGCTATTGGTTTAATTTGACCCATCTCTTCAAGTTTAATTTAAGTTTCATTATTGTTAGCTTAAACATATCACATAGGCTAACATAGTTTACTCTTGTTGTTATAAATAGGGCAAGAAACTTTAGAGTCACTAAAA

Coding sequence (CDS)

TCTTTTATTATAAAAACTCATCCACTCCTTACAACCAGCTGCTTGTTTTCTTTTCACACCAAGTCTATTATTCCCCCAATGGCCAGCTTCCCTTATGAAGATTCAAACCTTAACCCTAACCCTAACCCTAATAACTATACTCATTTTCCTCCGATTCTCGATCCCGCTTCTTTCTTTGATTTTGAGCTCTCTGATTTCCTTTTGTTTGGCGATGACAACAATATCGTCGTCGACCAAGTTGCATCGTCCTCGCCAAGTATGACTTCGTCCGAGAAGATCACCAGTGGAGGAGTTGATAGTGGTGGTAGCTCTACTGTAATTGATACCGGATCTAGCGTAGTAGTTTCTTCGTCGGGAGCGTCAACGACATCTATAAGAAGCAAAAATGGAGAGAAGAAAAGGAAAGGGGAAATGGGATGTAGAGTTGCATTTAGAACCAAATCAGAGCAAGAAATCATGGATGATGGCTACAAGTGGAGGAAGTATGGGAAAAAATCTGTGAAGAATAGTCCCAATCCCAGAAATTACTATAAATGTTCAAGTGAAGGATGCAATGTGAAGAAGAAGGTGGAAAGAGACAGAGAGGATGCAAACTATGTGATTACTACTTATGAGGGAATTCACAACCATGAAAGCCCCTTCGTTGTGTATTACAATCAATTGCCATCCTTTACCTCTGCCTCTACTCCCACCTAG

Protein sequence

SFIIKTHPLLTTSCLFSFHTKSIIPPMASFPYEDSNLNPNPNPNNYTHFPPILDPASFFDFELSDFLLFGDDNNIVVDQVASSSPSMTSSEKITSGGVDSGGSSTVIDTGSSVVVSSSGASTTSIRSKNGEKKRKGEMGCRVAFRTKSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDANYVITTYEGIHNHESPFVVYYNQLPSFTSASTPT

Homology

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BLAST of Cucsat.G15977.T1 vs. ExPASy Swiss-Prot
Match: Q93WU9 (Probable WRKY transcription factor 51 OS=Arabidopsis thaliana OX=3702 GN=WRKY51 PE=1 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 4.1e-34
Identity = 95/195 (48.72%), Postives = 128/195 (65.64%), Query Frame = 0


Query: 36  NLNPNPNPNNYTHFP------PILDPASFFDFELSDFLLFGDD---NNIVVDQVASSSPS 95
           N++ NP+P N+T+F       P +D   F     S+ + F  D   NN ++++  SS  S
Sbjct: 2   NISQNPSP-NFTYFSDENFINPFMDNNDF-----SNLMFFDIDEGGNNGLIEEEISSPTS 61

Query: 96  MTSSEKITSGGVDSGGSSTVIDTGSSVVVSSSGASTTSIRSKNGEKKRKGEMGCRVAFRT 155
           + SSE  T    +SGGS      GS+  +S     +T+  SK  ++ +  E G RVAFRT
Sbjct: 62  IVSSETFTG---ESGGS------GSATTLSKK--ESTNRGSKESDQTK--ETGHRVAFRT 121

Query: 156 KSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDANYVITTYEG 215
           +S+ ++MDDG+KWRKYGKKSVKN+ N RNYYKCSSEGC+VKK+VERD +DA YVITTYEG
Sbjct: 122 RSKIDVMDDGFKWRKYGKKSVKNNINKRNYYKCSSEGCSVKKRVERDGDDAAYVITTYEG 177

Query: 216 IHNHESPFVVYYNQL 222
           +HNHES   VYYN++
Sbjct: 182 VHNHESLSNVYYNEM 177

[Close]

BLAST of Cucsat.G15977.T1 vs. ExPASy Swiss-Prot
Match: Q8VWQ5 (Probable WRKY transcription factor 50 OS=Arabidopsis thaliana OX=3702 GN=WRKY50 PE=2 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.8e-26
Identity = 79/179 (44.13%), Postives = 112/179 (62.57%), Query Frame = 0


Query: 34  DSNLNPNPNPNNYTHFPPILDPASFFDFELSDFLLFGDDNNIVVDQVASSSPSMTSSEKI 93
           D+NL  + + + ++ F        F + +LSD  +  DD+  +V  V+  + S       
Sbjct: 5   DTNLGSSFSDDTHSVF-------EFPELDLSDEWM--DDD--LVSAVSGMNQSYGYQTSD 64

Query: 94  TSGGVDSGGSSTVIDTGSSVVVSSSGASTTSIRSKNGEKKRKGEMGCRVAFRTKSEQEIM 153
            +G + S GSS+      S    +  A+T +  + N  KK K ++  RVAF+T+SE E++
Sbjct: 65  VAGALFS-GSSSCFSHPESPSTKTYVAATATASADNQNKKEKKKIKGRVAFKTRSEVEVL 124

Query: 154 DDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDANYVITTYEGIHNHES 213
           DDG+KWRKYGKK VKNSP+PRNYYKCS +GC VKK+VERDR+D ++VITTYEG HNH S
Sbjct: 125 DDGFKWRKYGKKMVKNSPHPRNYYKCSVDGCPVKKRVERDRDDPSFVITTYEGSHNHSS 171

[Close]

BLAST of Cucsat.G15977.T1 vs. ExPASy Swiss-Prot
Match: Q9S763 (Probable WRKY transcription factor 45 OS=Arabidopsis thaliana OX=3702 GN=WRKY45 PE=1 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 3.3e-23
Identity = 52/96 (54.17%), Postives = 70/96 (72.92%), Query Frame = 0


Query: 115 VSSSGASTTSIRSKNGEKKRKGEMGCRVAFRTKSEQEIMDDGYKWRKYGKKSVKNSPNPR 174
           V +S   TTS   K   KK+K E   R AF+T+S+ +I+DDGY+WRKYG+K+VKN+P PR
Sbjct: 26  VDNSAQPTTSSEEKPRSKKKKKEREARYAFQTRSQVDILDDGYRWRKYGQKAVKNNPFPR 85

Query: 175 NYYKCSSEGCNVKKKVERDREDANYVITTYEGIHNH 211
           +YYKC+ EGC VKK+V+R   D   V+TTY+G+H H
Sbjct: 86  SYYKCTEEGCRVKKQVQRQWGDEGVVVTTYQGVHTH 121

[Close]

BLAST of Cucsat.G15977.T1 vs. ExPASy Swiss-Prot
Match: Q9SJ09 (Probable WRKY transcription factor 59 OS=Arabidopsis thaliana OX=3702 GN=WRKY59 PE=2 SV=2)

HSP 1 Score: 104.4 bits (259), Expect = 1.8e-21
Identity = 73/189 (38.62%), Postives = 106/189 (56.08%), Query Frame = 0


Query: 38  NPNPNPNNYTHFPPILDPASFFDFELSDFLLFGDDNNIVVDQVA----SSSP--SMTSSE 97
           NPNP+  ++T F           F+  D   F D    +++++     SSSP  S +SSE
Sbjct: 6   NPNPSSTDFTEF-----------FKFDD---FDDTFEKIMEEIGREDHSSSPTLSWSSSE 65

Query: 98  KITSGGVDSGGSSTVIDTGSSVVVSSSGASTTSIRSKNGEKKRKGEMGCRVAFRTKS--- 157
           K+ +  + S   +++  +  S  +          + +  ++KR  E      F+TKS   
Sbjct: 66  KLVAAEITSPLQTSLATSPMSFEIGD--------KDEIKKRKRHKEDPIIHVFKTKSSID 125

Query: 158 EQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDANYVITTYEGIH 217
           E+  +DDGYKWRKYGKK +  SP PR+Y+KCSS  CNVKKK+ERD  + +Y++TTYEG H
Sbjct: 126 EKVALDDGYKWRKYGKKPITGSPFPRHYHKCSSPDCNVKKKIERDTNNPDYILTTYEGRH 172

[Close]

BLAST of Cucsat.G15977.T1 vs. ExPASy Swiss-Prot
Match: Q93WV4 (WRKY transcription factor 71 OS=Arabidopsis thaliana OX=3702 GN=WRKY71 PE=2 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.2e-20
Identity = 63/136 (46.32%), Postives = 85/136 (62.50%), Query Frame = 0


Query: 80  VASSSPSMTSSEKITSGGVDSGGSSTVID--TGSSVVVSSSGASTTSIRSKNGEKKRKGE 139
           + S+SP ++SS        ++   S  ++   G    V  S    T    K GEKK   E
Sbjct: 63  LTSNSPVVSSSSNEGEPKENTNDKSDQMEDNEGDLHGVGESSKQLTKQGKKKGEKK---E 122

Query: 140 MGCRVAFRTKSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDA 199
              RVAF TKSE + ++DGY+WRKYG+K+VKNSP PR+YY+C+++ CNVKK+VER  +D 
Sbjct: 123 REVRVAFMTKSEIDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTTQKCNVKKRVERSFQDP 182

Query: 200 NYVITTYEGIHNHESP 214
           + VITTYEG HNH  P
Sbjct: 183 SIVITTYEGKHNHPIP 195

[Close]

BLAST of Cucsat.G15977.T1 vs. NCBI nr
Match: XP_004148451.1 (probable WRKY transcription factor 51 [Cucumis sativus] >KAE8651959.1 hypothetical protein Csa_006699 [Cucumis sativus])

HSP 1 Score: 402 bits (1033), Expect = 9.38e-141
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 0


Query: 27  MASFPYEDSNLNPNPNPNNYTHFPPILDPASFFDFELSDFLLFGDDNNIVVDQVASSSPS 86
           MASFPYEDSNLNPNPNPNNYTHFPPILDPASFFDFELSDFLLFGDDNNIVVDQVASSSPS
Sbjct: 1   MASFPYEDSNLNPNPNPNNYTHFPPILDPASFFDFELSDFLLFGDDNNIVVDQVASSSPS 60

Query: 87  MTSSEKITSGGVDSGGSSTVIDTGSSVVVSSSGASTTSIRSKNGEKKRKGEMGCRVAFRT 146
           MTSSEKITSGGVDSGGSSTVIDTGSSVVVSSSGASTTSIRSKNGEKKRKGEMGCRVAFRT
Sbjct: 61  MTSSEKITSGGVDSGGSSTVIDTGSSVVVSSSGASTTSIRSKNGEKKRKGEMGCRVAFRT 120

Query: 147 KSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDANYVITTYEG 206
           KSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDANYVITTYEG
Sbjct: 121 KSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDANYVITTYEG 180

Query: 207 IHNHESPFVVYYNQLPSFTSASTPT 231
           IHNHESPFVVYYNQLPSFTSASTPT
Sbjct: 181 IHNHESPFVVYYNQLPSFTSASTPT 205

[Close]

BLAST of Cucsat.G15977.T1 vs. NCBI nr
Match: XP_008457906.1 (PREDICTED: probable WRKY transcription factor 51 [Cucumis melo])

HSP 1 Score: 347 bits (889), Expect = 1.05e-118
Identity = 191/214 (89.25%), Postives = 196/214 (91.59%), Query Frame = 0


Query: 27  MASFPYEDSNLNPN---PNPNNY-THFPPILDPASFFDFELSDFLLFGDDNNI----VVD 86
           MASFPYEDSN NPN   PNPN+Y T FPPILDPASFFDFELSDFLLF D+NN     +VD
Sbjct: 1   MASFPYEDSNPNPNNLNPNPNHYYTPFPPILDPASFFDFELSDFLLFDDNNNNNNNNIVD 60

Query: 87  QVASSSPSMTSSEKITSGG-VDSGGSSTVIDTGSSVVVSSSGASTTSIRSKNGEKKRKGE 146
           Q ASSSPSMTSSEKIT GG VDS GSSTVIDTGSS+VVSS GASTTSIRSKNGEKKRKGE
Sbjct: 61  QAASSSPSMTSSEKITGGGGVDSSGSSTVIDTGSSIVVSS-GASTTSIRSKNGEKKRKGE 120

Query: 147 MGCRVAFRTKSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDA 206
           MGCRVAFRTKSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDA
Sbjct: 121 MGCRVAFRTKSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDA 180

Query: 207 NYVITTYEGIHNHESPFVVYYNQLPSFTSASTPT 231
           NYVITTYEGIHNHESPFVVYYNQLPSFT+ STPT
Sbjct: 181 NYVITTYEGIHNHESPFVVYYNQLPSFTT-STPT 212

[Close]

BLAST of Cucsat.G15977.T1 vs. NCBI nr
Match: XP_038901292.1 (probable WRKY transcription factor 51 isoform X1 [Benincasa hispida])

HSP 1 Score: 330 bits (845), Expect = 3.85e-112
Identity = 181/210 (86.19%), Postives = 189/210 (90.00%), Query Frame = 0


Query: 27  MASFPYEDSNLNPNPNPN-----NYTHFPPILDPASFFDFELSDFLLFGDDNNIVVDQVA 86
           MASFPYEDSN N NPN N     NYTHFPPILDP+SFFDFE+SDF +F DDNN V DQVA
Sbjct: 1   MASFPYEDSNPNLNPNSNLNPNPNYTHFPPILDPSSFFDFEMSDFFVFDDDNNNV-DQVA 60

Query: 87  SSSPSMTSSEKITSGGVDSGGSSTVIDTGSSVVVSSSGASTTSIRSKNGEKKRKGEMGCR 146
           SSSPSMTSSEKIT   VDSGGSS VID+GSS+VVSS GAST SIRSKNGEKKRKGEMGCR
Sbjct: 61  SSSPSMTSSEKITE--VDSGGSSAVIDSGSSLVVSS-GAST-SIRSKNGEKKRKGEMGCR 120

Query: 147 VAFRTKSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDANYVI 206
           VAFRTKSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDA+YVI
Sbjct: 121 VAFRTKSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDASYVI 180

Query: 207 TTYEGIHNHESPFVVYYNQLPSFTSASTPT 231
           TTYEG+HNHESPFVVYYNQ+PSF S  TPT
Sbjct: 181 TTYEGVHNHESPFVVYYNQIPSFRS--TPT 203

[Close]

BLAST of Cucsat.G15977.T1 vs. NCBI nr
Match: XP_022927982.1 (probable WRKY transcription factor 51 [Cucurbita moschata])

HSP 1 Score: 295 bits (754), Expect = 1.69e-98
Identity = 163/203 (80.30%), Postives = 176/203 (86.70%), Query Frame = 0


Query: 27  MASFPYEDSNLNPNPNPNNYTHFPPILDPASFFDFELSDFLLFGDDNNIVVDQVASSSPS 86
           MASFPYED N NPNPN   +T F PILDPASFFDF+LS+FL+ GDD++ V DQ+ASSS  
Sbjct: 1   MASFPYEDPNPNPNPN---FTQFSPILDPASFFDFDLSNFLV-GDDDSYV-DQLASSS-- 60

Query: 87  MTSSEKITSGGVDSGGSSTVIDTGSSVVVSSSGASTTSIRSKNGEKKRKGEMGCRVAFRT 146
              SEKIT  GVDSGGSS  +D+GSS VVSS    + SIRSKNGE+KRKGEMGCRVAFRT
Sbjct: 61  ---SEKIT--GVDSGGSSVAVDSGSSFVVSSE--PSPSIRSKNGERKRKGEMGCRVAFRT 120

Query: 147 KSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDANYVITTYEG 206
           KSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDA+YVITTYEG
Sbjct: 121 KSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDASYVITTYEG 180

Query: 207 IHNHESPFVVYYNQLPSFTSAST 229
           IHNHESPFVVYYNQ+PS TS ST
Sbjct: 181 IHNHESPFVVYYNQIPSLTSTST 189

[Close]

BLAST of Cucsat.G15977.T1 vs. NCBI nr
Match: XP_023512174.1 (probable WRKY transcription factor 51 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 291 bits (745), Expect = 3.96e-97
Identity = 161/203 (79.31%), Postives = 175/203 (86.21%), Query Frame = 0


Query: 27  MASFPYEDSNLNPNPNPNNYTHFPPILDPASFFDFELSDFLLFGDDNNIVVDQVASSSPS 86
           MASFPYED N NPNPN   +  F PILDPASFFDF+LS+FL+ GDD++ V DQ+ASSS  
Sbjct: 1   MASFPYEDPNPNPNPN---FMQFSPILDPASFFDFDLSNFLV-GDDDSYV-DQLASSS-- 60

Query: 87  MTSSEKITSGGVDSGGSSTVIDTGSSVVVSSSGASTTSIRSKNGEKKRKGEMGCRVAFRT 146
              SEKIT  G+DSGGSS  +D+GSS VVSS    + SIRSKNGE+KRKGEMGCRVAFRT
Sbjct: 61  ---SEKIT--GMDSGGSSVAVDSGSSFVVSSE--PSPSIRSKNGERKRKGEMGCRVAFRT 120

Query: 147 KSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDANYVITTYEG 206
           KSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDA+YVITTYEG
Sbjct: 121 KSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDASYVITTYEG 180

Query: 207 IHNHESPFVVYYNQLPSFTSAST 229
           IHNHESPFVVYYNQ+PS TS ST
Sbjct: 181 IHNHESPFVVYYNQIPSLTSTST 189

[Close]

BLAST of Cucsat.G15977.T1 vs. ExPASy TrEMBL
Match: A0A0A0LM41 (WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G297180 PE=4 SV=1)

HSP 1 Score: 389 bits (998), Expect = 8.21e-136
Identity = 200/205 (97.56%), Postives = 200/205 (97.56%), Query Frame = 0


Query: 27  MASFPYEDSNLNPNPNPNNYTHFPPILDPASFFDFELSDFLLFGDDNNIVVDQVASSSPS 86
           MASFPYEDSNLNPNPNPNNYTHFPPILDPASFFDFELSDFLLFGDDNNIVVDQVASSSPS
Sbjct: 1   MASFPYEDSNLNPNPNPNNYTHFPPILDPASFFDFELSDFLLFGDDNNIVVDQVASSSPS 60

Query: 87  MTSSEKITSGGVDSGGSSTVIDTGSSVVVSSSGASTTSIRSKNGEKKRKGEMGCRVAFRT 146
           MTSSEKITSGGVDSGGSSTVIDTGSSVVVSSSGA     RSKNGEKKRKGEMGCRVAFRT
Sbjct: 61  MTSSEKITSGGVDSGGSSTVIDTGSSVVVSSSGA-----RSKNGEKKRKGEMGCRVAFRT 120

Query: 147 KSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDANYVITTYEG 206
           KSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDANYVITTYEG
Sbjct: 121 KSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDANYVITTYEG 180

Query: 207 IHNHESPFVVYYNQLPSFTSASTPT 231
           IHNHESPFVVYYNQLPSFTSASTPT
Sbjct: 181 IHNHESPFVVYYNQLPSFTSASTPT 200

[Close]

BLAST of Cucsat.G15977.T1 vs. ExPASy TrEMBL
Match: A0A1S3C6R2 (probable WRKY transcription factor 51 OS=Cucumis melo OX=3656 GN=LOC103497478 PE=4 SV=1)

HSP 1 Score: 347 bits (889), Expect = 5.09e-119
Identity = 191/214 (89.25%), Postives = 196/214 (91.59%), Query Frame = 0


Query: 27  MASFPYEDSNLNPN---PNPNNY-THFPPILDPASFFDFELSDFLLFGDDNNI----VVD 86
           MASFPYEDSN NPN   PNPN+Y T FPPILDPASFFDFELSDFLLF D+NN     +VD
Sbjct: 1   MASFPYEDSNPNPNNLNPNPNHYYTPFPPILDPASFFDFELSDFLLFDDNNNNNNNNIVD 60

Query: 87  QVASSSPSMTSSEKITSGG-VDSGGSSTVIDTGSSVVVSSSGASTTSIRSKNGEKKRKGE 146
           Q ASSSPSMTSSEKIT GG VDS GSSTVIDTGSS+VVSS GASTTSIRSKNGEKKRKGE
Sbjct: 61  QAASSSPSMTSSEKITGGGGVDSSGSSTVIDTGSSIVVSS-GASTTSIRSKNGEKKRKGE 120

Query: 147 MGCRVAFRTKSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDA 206
           MGCRVAFRTKSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDA
Sbjct: 121 MGCRVAFRTKSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDA 180

Query: 207 NYVITTYEGIHNHESPFVVYYNQLPSFTSASTPT 231
           NYVITTYEGIHNHESPFVVYYNQLPSFT+ STPT
Sbjct: 181 NYVITTYEGIHNHESPFVVYYNQLPSFTT-STPT 212

[Close]

BLAST of Cucsat.G15977.T1 vs. ExPASy TrEMBL
Match: A0A6J1EIN3 (probable WRKY transcription factor 51 OS=Cucurbita moschata OX=3662 GN=LOC111434887 PE=4 SV=1)

HSP 1 Score: 295 bits (754), Expect = 8.18e-99
Identity = 163/203 (80.30%), Postives = 176/203 (86.70%), Query Frame = 0


Query: 27  MASFPYEDSNLNPNPNPNNYTHFPPILDPASFFDFELSDFLLFGDDNNIVVDQVASSSPS 86
           MASFPYED N NPNPN   +T F PILDPASFFDF+LS+FL+ GDD++ V DQ+ASSS  
Sbjct: 1   MASFPYEDPNPNPNPN---FTQFSPILDPASFFDFDLSNFLV-GDDDSYV-DQLASSS-- 60

Query: 87  MTSSEKITSGGVDSGGSSTVIDTGSSVVVSSSGASTTSIRSKNGEKKRKGEMGCRVAFRT 146
              SEKIT  GVDSGGSS  +D+GSS VVSS    + SIRSKNGE+KRKGEMGCRVAFRT
Sbjct: 61  ---SEKIT--GVDSGGSSVAVDSGSSFVVSSE--PSPSIRSKNGERKRKGEMGCRVAFRT 120

Query: 147 KSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDANYVITTYEG 206
           KSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDA+YVITTYEG
Sbjct: 121 KSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDASYVITTYEG 180

Query: 207 IHNHESPFVVYYNQLPSFTSAST 229
           IHNHESPFVVYYNQ+PS TS ST
Sbjct: 181 IHNHESPFVVYYNQIPSLTSTST 189

[Close]

BLAST of Cucsat.G15977.T1 vs. ExPASy TrEMBL
Match: A0A6J1DV80 (probable WRKY transcription factor 51 OS=Momordica charantia OX=3673 GN=LOC111024371 PE=4 SV=1)

HSP 1 Score: 285 bits (730), Expect = 3.67e-95
Identity = 151/199 (75.88%), Postives = 165/199 (82.91%), Query Frame = 0


Query: 27  MASFPYEDSNLNPNPNPNNYTHFPPILDPASFFDFELSDFLLFGDDNNIVVDQVASSSPS 86
           MASFP +D N NPNPN   YTHFPPILDP+ FFDF+LSD+L+  DDN  + DQ ASSSPS
Sbjct: 1   MASFPQQDPNPNPNPN---YTHFPPILDPSVFFDFDLSDYLMLNDDNINIDDQAASSSPS 60

Query: 87  MTSSEKITSGGVDSGGSSTVIDTGSSVVVSSSGASTTSIRSKNGEKKRKGEMGCRVAFRT 146
           + SSEKIT  GVDSG S  V+D+GSS V SS   S     S N  KK+K ++GCRVAFRT
Sbjct: 61  IASSEKIT--GVDSGNSCAVVDSGSSTVASSGATS-----SMNEGKKKKEDVGCRVAFRT 120

Query: 147 KSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDANYVITTYEG 206
           KSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGC VKKKVERDREDA+YVITTYEG
Sbjct: 121 KSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCTVKKKVERDREDASYVITTYEG 180

Query: 207 IHNHESPFVVYYNQLPSFT 225
           IHNHESPFVVYYNQ+PSFT
Sbjct: 181 IHNHESPFVVYYNQMPSFT 189

[Close]

BLAST of Cucsat.G15977.T1 vs. ExPASy TrEMBL
Match: A0A6J1I2S4 (probable WRKY transcription factor 51 OS=Cucurbita maxima OX=3661 GN=LOC111470401 PE=4 SV=1)

HSP 1 Score: 281 bits (720), Expect = 1.22e-93
Identity = 155/203 (76.35%), Postives = 171/203 (84.24%), Query Frame = 0


Query: 27  MASFPYEDSNLNPNPNPNNYTHFPPILDPASFFDFELSDFLLFGDDNNIVVDQVASSSPS 86
           MASFPYED N NPNPN   +T F PILDPASFFDF+LS+FL+ GDD++ V DQ+ASSS  
Sbjct: 1   MASFPYEDPNPNPNPN---FTQFSPILDPASFFDFDLSNFLV-GDDDSYV-DQLASSS-- 60

Query: 87  MTSSEKITSGGVDSGGSSTVIDTGSSVVVSSSGASTTSIRSKNGEKKRKGEMGCRVAFRT 146
              SEKI   GVDSGGSS  +D+GSS VVSS    + SIRSK GE+K KGEMGCR+AFRT
Sbjct: 61  ---SEKII--GVDSGGSSVAVDSGSSFVVSSE--PSPSIRSKKGERKMKGEMGCRIAFRT 120

Query: 147 KSEQEIMDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDANYVITTYEG 206
           KSEQEI+DDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDRED +YVITTYEG
Sbjct: 121 KSEQEIIDDGYKWRKYGKKSVKNSPNPRNYYKCSSEGCNVKKKVERDREDTSYVITTYEG 180

Query: 207 IHNHESPFVVYYNQLPSFTSAST 229
           IHNHESPF +YYNQ+PS TS ST
Sbjct: 181 IHNHESPFALYYNQIPSLTSTST 189

The following BLAST results are available for this feature:

BLAST of Cucsat.G15977.T1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93WU9	4.1e-34	48.72	Probable WRKY transcription factor 51 OS=Arabidopsis thaliana OX=3702 GN=WRKY51 ...	[more]
Q8VWQ5	1.8e-26	44.13	Probable WRKY transcription factor 50 OS=Arabidopsis thaliana OX=3702 GN=WRKY50 ...	[more]
Q9S763	3.3e-23	54.17	Probable WRKY transcription factor 45 OS=Arabidopsis thaliana OX=3702 GN=WRKY45 ...	[more]
Q9SJ09	1.8e-21	38.62	Probable WRKY transcription factor 59 OS=Arabidopsis thaliana OX=3702 GN=WRKY59 ...	[more]
Q93WV4	1.2e-20	46.32	WRKY transcription factor 71 OS=Arabidopsis thaliana OX=3702 GN=WRKY71 PE=2 SV=1	[more]

BLAST of Cucsat.G15977.T1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004148451.1	9.38e-141	100.00	probable WRKY transcription factor 51 [Cucumis sativus] >KAE8651959.1 hypothetic...	[more]
XP_008457906.1	1.05e-118	89.25	PREDICTED: probable WRKY transcription factor 51 [Cucumis melo]	[more]
XP_038901292.1	3.85e-112	86.19	probable WRKY transcription factor 51 isoform X1 [Benincasa hispida]	[more]
XP_022927982.1	1.69e-98	80.30	probable WRKY transcription factor 51 [Cucurbita moschata]	[more]
XP_023512174.1	3.96e-97	79.31	probable WRKY transcription factor 51 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cucsat.G15977.T1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LM41	8.21e-136	97.56	WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G297180 PE=4 S...	[more]
A0A1S3C6R2	5.09e-119	89.25	probable WRKY transcription factor 51 OS=Cucumis melo OX=3656 GN=LOC103497478 PE...	[more]
A0A6J1EIN3	8.18e-99	80.30	probable WRKY transcription factor 51 OS=Cucurbita moschata OX=3662 GN=LOC111434...	[more]
A0A6J1DV80	3.67e-95	75.88	probable WRKY transcription factor 51 OS=Momordica charantia OX=3673 GN=LOC11102...	[more]
A0A6J1I2S4	1.22e-93	76.35	probable WRKY transcription factor 51 OS=Cucurbita maxima OX=3661 GN=LOC11147040...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003657	WRKY domain	SMART	SM00774	WRKY_cls	coord: 153..212 e-value: 5.6E-35 score: 132.2
IPR003657	WRKY domain	PFAM	PF03106	WRKY	coord: 154..211 e-value: 2.2E-23 score: 82.3
IPR003657	WRKY domain	PROSITE	PS50811	WRKY	coord: 148..213 score: 31.754583
IPR036576	WRKY domain superfamily	GENE3D	2.20.25.80	WRKY domain	coord: 128..214 e-value: 4.6E-32 score: 111.6
IPR036576	WRKY domain superfamily	SUPERFAMILY	118290	WRKY DNA-binding domain	coord: 146..213
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 118..137
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 82..103
None	No IPR available	PANTHER	PTHR31221:SF219	WRKY TRANSCRIPTION FACTOR 50-RELATED	coord: 36..219
IPR044810	WRKY transcription factor, plant	PANTHER	PTHR31221	WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATED	coord: 36..219

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G15977	Cucsat.G15977	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G15977.T1.E1	Cucsat.G15977.T1.E1	exon
Cucsat.G15977.T1.E2	Cucsat.G15977.T1.E2	exon
Cucsat.G15977.T1.E3	Cucsat.G15977.T1.E3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G15977.T1.C3	Cucsat.G15977.T1.C3	CDS
Cucsat.G15977.T1.C2	Cucsat.G15977.T1.C2	CDS
Cucsat.G15977.T1.C1	Cucsat.G15977.T1.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G15977.T1	Cucsat.G15977.T1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0050896	response to stimulus
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0043565	sequence-specific DNA binding