Cucsat.G15678.T16 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G15678.T16
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionNAD(P)-binding Rossmann-fold superfamily protein
Locationctg2009: 5635277 .. 5637794 (+)
RNA-Seq ExpressionCucsat.G15678.T16
SyntenyCucsat.G15678.T16
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTAATTTTCTTAAACGATTTCACAACTTTTATTAAGGGATCTTCCAACCTATACTAAGCAATCTCTCATCTTTTATTAATTAGTTTCTTAACTTTCATTACCCAATCTCTCATGTTTATCAAAGGAGGCTCAACTTTTATTGAGTGATCTTCCAAATTTTATTAAAACATTTATAGAAAGACAAACTGTAAGGAAGGAAGATAACGACAAGTGAGTTTCCATTTAATACTACTTTCCATTCCATAATAAGAGGTAAAAATGACCTTTCGTATGACAAAAAGGGGACAGGATGTATAAGTTTTATAGAAATGGGTAAAAGTTTTTGCCTTTGCGTTGGTAATATTTTACTTGTATTCCATTGTTTCGGGTCCTCTTCTCTAACTTTCTTATGAATTAACATGTTGAATAATCAACATTCAATTCATTTTGTACTCGTCAACTGTTGACTGCTATGCATATTTGAGGTGATTTTTTTCTATACCAATTTCCTGGAAATTCCATCTCTGTCTTTGTTTTCTCTTCATAATCCTTCAATGATTCTTCTTTTTTCCCCAGGAATTGAAGACCCATTTGCTTTCCAAGATGGAGGGATCGACCAAGAACGTCTTACTTTCTTCCGGTGGGGATGAGATCTCCAAGAATCTGGCTCTTCATCTTGCCAGAAGGGGTTGCAGGTTCAGCATCCAACTTATAACTTGATGGTTCTGTTTGCATATTTATTGAGGAAATTGAGAGATATGGAGGCATTCATACCTTTTGTTATGGGGTTTCTCTCTTCTGTTTTGTATGCCTCTTTAATGCATTTTTTATTCGCAGTTGTTAACCTTGTATCCATCTTTTTCATGGCTATGTCCAGATTAGTTTTGATTGGAAATGAGTGTGTTCTTCAGAGCATGAGTAAGATGATAGCGGAATCGTTAAAGGGTGTACTGCCAATTGAGGTTGTTGGGGTGGACATGGAAGAAGAGAGAGAGGCGGCTTTTGATGAGGCTGTGAACAGGGCATGCGGTATTTTGGGAACTTTGGATGCTTTCGTCCATGCTTATTCTTATGAAGGTACAACCCTTCTCTCTCTTTCCTCCATACTTTCTTTTCATTACAATGATGTAGTAAGGTTGCTATGTTGTTTTTGGTTTGATTGTTTAGGGTTCTTTATATTAGTTAGGTGTTGATTCTTTGTTTGGGCATGTTTTGATATTGTGGGGAAGATTGAATACAGATTTTCGGTGTATATTGGAAAGAGGTTAGGCTACACCATCCTGACTTTTCTGAAGTATGTGCTCATAGCTTTTGGTGCACTCAGTGACTGTTTACAGATATCGGTTTATTTCACTGAGCTTAGAACTGAAGCTAGAAGGGTCTTTCTGAGAGTTAATGGTTTTAGTATGGATTCTAATTTCAAATGAGAATTTACGTTCAAAAGGCTTCTGAGGCATATGTTTTCAGGTTGCCTTCGGGCCTCGGGGTAGCTTCAAACCTTATCCCTCCCTTGTGTTGTAAATACAACAGGCACAAAGTCTTTTGTTGCGCCTTTGTGAGAATGCAATGGGGGCTTGAGGCTTACAATATGTATGCCTATGTAAGCCTATGTAATAGTTAGAAGAATGATGTTAAATTGTTCTCTTGAAAAAAGTTTTCATATTTACCATTGCGTATGTGTGTATATGTGGTTTTTTTCTTTCTTTAAAATTCAAATTAAGTCTTATGCCTCATGCTTCCCTCAAAGAAAATAAAATTAAAAATAATGCAATGCAGCTTTTAGCCGTTTAAAGCATTGGCAAGAACAGAAAGGAGTTGTTCTCTTAAGCCTTAATTGTGTGTGTATATGTACTTTTTTTATCTGTATAGAGTTTCTTCCATTTTTCCTATGGTATATTGATTTTTAATTGTTTAGTATCACTCAATATTTGACAAAAGTAGTTGACAAATGATCTTTAGAAGTCCACTCTGTTCTACTCATAAGAAAAGTCTGCTGCAAGTAAATGGTGTATCAACAAGCGGAACATCGTGAAAATCTTTTTTTCTTCAGGACAAAGTTTTGAAGAGCCCTCCCTTTCTTAATGAAGAACCAAGGAATTACCGTCCATTTTATCTTCCATAAATTTCTTCGACATGTCTTTTGTGGATGCTCCATGCAACAATTAAGAACACTTAAAATGTAATTATTGCTATCTTGGTTTGAAATGAAATAAACTTCATTTACAAAAAAAATAGGATTTTATCAGTACACTTGTCATCCTCTCTGTCGAGCCATTGAGACAATATGATGCCTTTTAATAGAACCAAGCCAGAATTTAAGGAAAAACAATTAGCTTTTAAAAACTAGTGCCACGTACAATTGTATGCATATTAATTGGTTTACATTTTCTTTAAGATATAATACTGAACTCTGCTATTGGATGATGAAAATTAGGATGTAGAAATTCTTTGAAAGCTATTCTACTGTAAACCGCGTTAACTGCACTGTGTTTGAAGCAGGTCCCATACAAGATGCTTTACAACTATCTGAAGAGGAAT

Coding sequence (CDS)

ATGGAGGGATCGACCAAGAACGTCTTACTTTCTTCCGGTGGGGATGAGATCTCCAAGAATCTGGCTCTTCATCTTGCCAGAAGGGGTTGCAGATTAGTTTTGATTGGAAATGAGTGTGTTCTTCAGAGCATGAGTAAGATGATAGCGGAATCGTTAAAGGGTGTACTGCCAATTGAGGTTGTTGGGGTGGACATGGAAGAAGAGAGAGAGGCGGCTTTTGATGAGGCTGTGAACAGGGCATGCGGTATTTTGGGAACTTTGGATGCTTTCGTCCATGCTTATTCTTATGAAGAAGTCCACTCTGTTCTACTCATAAGAAAAGTCTGCTGCAAGTAA

Protein sequence

MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEVVGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYEEVHSVLLIRKVCCK
Homology
BLAST of Cucsat.G15678.T16 vs. NCBI nr
Match: XP_011651549.1 (uncharacterized protein LOC101219109 [Cucumis sativus] >KGN58163.1 hypothetical protein Csa_017656 [Cucumis sativus])

HSP 1 Score: 192 bits (488), Expect = 4.78e-59
Identity = 97/97 (100.00%), Postives = 97/97 (100.00%), Query Frame = 0

Query: 1  MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60
          MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV
Sbjct: 1  MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60

Query: 61 VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYE 97
          VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYE
Sbjct: 61 VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYE 97

BLAST of Cucsat.G15678.T16 vs. NCBI nr
Match: KAA0060247.1 (carbonyl reductase [Cucumis melo var. makuwa] >TYJ97611.1 carbonyl reductase [Cucumis melo var. makuwa])

HSP 1 Score: 187 bits (475), Expect = 4.32e-57
Identity = 93/97 (95.88%), Postives = 96/97 (98.97%), Query Frame = 0

Query: 1  MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60
          MEG+TKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV
Sbjct: 1  MEGATKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60

Query: 61 VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYE 97
          VG+DMEEEREAAFDEAVNRAC +LGTLDAFVHAYSYE
Sbjct: 61 VGLDMEEEREAAFDEAVNRACSVLGTLDAFVHAYSYE 97

BLAST of Cucsat.G15678.T16 vs. NCBI nr
Match: NP_001284466.1 (carbonyl reductase [NADPH] 2 [Cucumis melo] >ABC02082.1 putative alcohol dehydrogenases [Cucumis melo])

HSP 1 Score: 187 bits (475), Expect = 4.45e-57
Identity = 93/97 (95.88%), Postives = 96/97 (98.97%), Query Frame = 0

Query: 1  MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60
          MEG+TKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV
Sbjct: 1  MEGATKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60

Query: 61 VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYE 97
          VG+DMEEEREAAFDEAVNRAC +LGTLDAFVHAYSYE
Sbjct: 61 VGLDMEEEREAAFDEAVNRACSVLGTLDAFVHAYSYE 97

BLAST of Cucsat.G15678.T16 vs. NCBI nr
Match: XP_038878089.1 (levodione reductase-like [Benincasa hispida])

HSP 1 Score: 169 bits (427), Expect = 1.37e-49
Identity = 84/97 (86.60%), Postives = 92/97 (94.85%), Query Frame = 0

Query: 1   MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60
           ME +TKNVLLSS GD+ISKNLA+HLA+RGCRLVLIGNE VLQSMSK++AESLKGVLPIEV
Sbjct: 20  MERATKNVLLSSNGDDISKNLAIHLAKRGCRLVLIGNEKVLQSMSKVMAESLKGVLPIEV 79

Query: 61  VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYE 97
           VG+DMEEEREAAFD+AVNRAC ILGTLDAF HAYSYE
Sbjct: 80  VGLDMEEEREAAFDKAVNRACSILGTLDAFAHAYSYE 116

BLAST of Cucsat.G15678.T16 vs. NCBI nr
Match: XP_023529292.1 (carbonyl reductase [NADPH] 2-like [Cucurbita pepo subsp. pepo] >XP_023529293.1 carbonyl reductase [NADPH] 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 160 bits (404), Expect = 2.40e-46
Identity = 82/108 (75.93%), Postives = 92/108 (85.19%), Query Frame = 0

Query: 1   MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60
           ME +TKNVLLSS GDEISKNLA+HLA+RGCRLVL+GNE VLQSM + I ESLKGVLPI V
Sbjct: 1   MERATKNVLLSSNGDEISKNLAIHLAKRGCRLVLVGNETVLQSMREGIVESLKGVLPIGV 60

Query: 61  VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYE-EVHSVLLIRK 107
           VG+DME EREAAFDEAVN AC +LGTLDAFVHAYSYE  +  VL + +
Sbjct: 61  VGLDMEGEREAAFDEAVNGACSVLGTLDAFVHAYSYEGPIQEVLQLSE 108

BLAST of Cucsat.G15678.T16 vs. ExPASy TrEMBL
Match: A0A0A0L8L9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G566330 PE=4 SV=1)

HSP 1 Score: 192 bits (488), Expect = 2.31e-59
Identity = 97/97 (100.00%), Postives = 97/97 (100.00%), Query Frame = 0

Query: 1  MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60
          MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV
Sbjct: 1  MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60

Query: 61 VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYE 97
          VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYE
Sbjct: 61 VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYE 97

BLAST of Cucsat.G15678.T16 vs. ExPASy TrEMBL
Match: A0A5A7V0A0 (Carbonyl reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold639G00030 PE=4 SV=1)

HSP 1 Score: 187 bits (475), Expect = 2.09e-57
Identity = 93/97 (95.88%), Postives = 96/97 (98.97%), Query Frame = 0

Query: 1  MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60
          MEG+TKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV
Sbjct: 1  MEGATKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60

Query: 61 VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYE 97
          VG+DMEEEREAAFDEAVNRAC +LGTLDAFVHAYSYE
Sbjct: 61 VGLDMEEEREAAFDEAVNRACSVLGTLDAFVHAYSYE 97

BLAST of Cucsat.G15678.T16 vs. ExPASy TrEMBL
Match: Q2PZA4 (Putative alcohol dehydrogenases OS=Cucumis melo OX=3656 GN=ADH2 PE=2 SV=1)

HSP 1 Score: 187 bits (475), Expect = 2.15e-57
Identity = 93/97 (95.88%), Postives = 96/97 (98.97%), Query Frame = 0

Query: 1  MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60
          MEG+TKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV
Sbjct: 1  MEGATKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60

Query: 61 VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYE 97
          VG+DMEEEREAAFDEAVNRAC +LGTLDAFVHAYSYE
Sbjct: 61 VGLDMEEEREAAFDEAVNRACSVLGTLDAFVHAYSYE 97

BLAST of Cucsat.G15678.T16 vs. ExPASy TrEMBL
Match: A0A6J1DTS1 (uncharacterized protein LOC111023414 OS=Momordica charantia OX=3673 GN=LOC111023414 PE=4 SV=1)

HSP 1 Score: 159 bits (403), Expect = 1.65e-46
Identity = 80/97 (82.47%), Postives = 90/97 (92.78%), Query Frame = 0

Query: 1  MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60
          ME +TKNVLLSS GDEISK+LA++LA+RGCRLVLIGNE +LQS SK+I ESLKGVLPI+V
Sbjct: 1  MERATKNVLLSSNGDEISKSLAINLAKRGCRLVLIGNESLLQSTSKVIVESLKGVLPIDV 60

Query: 61 VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYE 97
          VG+DMEEEREAAFDEAVNRAC ILG LDAFV+AYSYE
Sbjct: 61 VGLDMEEEREAAFDEAVNRACSILGALDAFVNAYSYE 97

BLAST of Cucsat.G15678.T16 vs. ExPASy TrEMBL
Match: A0A6J1EJP8 (carbonyl reductase [NADPH] 2-like OS=Cucurbita moschata OX=3662 GN=LOC111435110 PE=4 SV=1)

HSP 1 Score: 158 bits (399), Expect = 6.60e-46
Identity = 80/108 (74.07%), Postives = 92/108 (85.19%), Query Frame = 0

Query: 1   MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60
           MEG+TKNVLLSS GDEISKNLA+HLA+RGCRLVL+GNE VL+SM + I ESLKG LPI V
Sbjct: 1   MEGATKNVLLSSNGDEISKNLAIHLAKRGCRLVLVGNETVLRSMREGIVESLKGALPIGV 60

Query: 61  VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYE-EVHSVLLIRK 107
           VG+DME EREAAFDEAV+ AC +LGTLDAFVHAYSYE  +  VL + +
Sbjct: 61  VGLDMEGEREAAFDEAVSGACSVLGTLDAFVHAYSYEGPIQEVLQLSE 108

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
XP_011651549.14.78e-59100.00uncharacterized protein LOC101219109 [Cucumis sativus] >KGN58163.1 hypothetical ... [more]
KAA0060247.14.32e-5795.88carbonyl reductase [Cucumis melo var. makuwa] >TYJ97611.1 carbonyl reductase [Cu... [more]
NP_001284466.14.45e-5795.88carbonyl reductase [NADPH] 2 [Cucumis melo] >ABC02082.1 putative alcohol dehydro... [more]
XP_038878089.11.37e-4986.60levodione reductase-like [Benincasa hispida][more]
XP_023529292.12.40e-4675.93carbonyl reductase [NADPH] 2-like [Cucurbita pepo subsp. pepo] >XP_023529293.1 c... [more]
Match NameE-valueIdentityDescription
A0A0A0L8L92.31e-59100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G566330 PE=4 SV=1[more]
A0A5A7V0A02.09e-5795.88Carbonyl reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold639G0... [more]
Q2PZA42.15e-5795.88Putative alcohol dehydrogenases OS=Cucumis melo OX=3656 GN=ADH2 PE=2 SV=1[more]
A0A6J1DTS11.65e-4682.47uncharacterized protein LOC111023414 OS=Momordica charantia OX=3673 GN=LOC111023... [more]
A0A6J1EJP86.60e-4674.07carbonyl reductase [NADPH] 2-like OS=Cucurbita moschata OX=3662 GN=LOC111435110 ... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 4..102
e-value: 6.3E-6
score: 27.7
NoneNo IPR availablePANTHERPTHR44375BETA-KETOACYL-ACP REDUCTASE-LIKE PROTEIN-RELATEDcoord: 1..103
NoneNo IPR availablePANTHERPTHR44375:SF6F28J7.36 PROTEINcoord: 1..103
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 5..95

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G15678Cucsat.G15678gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G15678.T16.E1Cucsat.G15678.T16.E1exon
Cucsat.G15678.T16.E2Cucsat.G15678.T16.E2exon
Cucsat.G15678.T16.E3Cucsat.G15678.T16.E3exon
Cucsat.G15678.T16.E4Cucsat.G15678.T16.E4exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G15678.T16.C1Cucsat.G15678.T16.C1CDS
Cucsat.G15678.T16.C2Cucsat.G15678.T16.C2CDS
Cucsat.G15678.T16.C3Cucsat.G15678.T16.C3CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G15678.T16Cucsat.G15678.T16-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004090 carbonyl reductase (NADPH) activity