Cucsat.G15606.T10 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G15606.T10
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Descriptioncalcineurin B-like protein 4
Locationctg2009: 2908615 .. 2913138 (+)
RNA-Seq ExpressionCucsat.G15606.T10
SyntenyCucsat.G15606.T10
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GGGGGAGCAGATCAAGTGGACGGAGCCTATAAATTACATGAAAGGGACTTTCTAAAACTTTCAACCCATTATCAACACAAAATCAAGCTTTCAAATGGTCGCCGGAGCTTTCTTAGAATCTCTCTACTAAGATTTCGGTATTGTCTCTGTTTTATTTTCTTCTATTTCCAACTGGGTTCTTGTTTTTTATGCTATTCTCCACTGAAATGCAAGTTCTTTCTTTGTTTCTGCTCTGATCATTTCTCTTCTGTTTCATCTAAGCTAACAACAAATACTCGGATTGCCTTACTTTCCTTTCTATTTTTCTGCATTTATGGAGTTTCTTGTGAATTCCTTGAAAATATCAGCTTAGTTACATCAGACTTTAATGGTCCACTGATATAAAACTATATTATCTCTTTTGGGGGTTTTCCCGCTTGTAAAAAAGTTTTGTATTCTTTTGTGAGATTTGTGAGGAGAAAAAAGGCATTTCCATATTATTCAAGGTTTTGCTTCTAGATTTCATCATTCATGATGTTTACTTCCCCTTCTCCTTAATTTACGCCCGGGTAGCCATTTTGTTTTTGGTTTTTGATCAATGAAAATTAAGTTTGTTTTCTCTTAGGCTTTGGCATCTTTCTTAGGTACAAGAGTTGAAACTAGTTTTTAAATTCTTTAGTTTTTTTTAATTTGGTTAGATTTTTGAAAACACTGGTAAAAAATGGATAAACTTGAGTGAAACTGATGTTTATAGGCTAAATTGTTTGGTAACCATTTGGTTAGTTTTTGAGGATTAAAACCTATAAACATCACTTTAACCTTTATCTTCCAAAACAAACCAAATTTTGATGTTTTTAGAATTTGGCCAAGTTTTCAAGTCTTGTACTTGAGAAACGATACAAACTAATGTAACAAAATGTGAGGAAATAAACTTACGAGATCTAAATGGATGCTAGGTGGGGTCTTAGAATGTAAACGTGGATTTCATTCCATTGAGCTACATGACAAAGGGAAAAGTAAAAGGTTGAACATTTAAAAAAAGCAAAAAAAGATGAAGGGGTTGGTCATATGATGCGATCCAGAATCAACTATGATTAGTTTAACACTTTCTGTTGAAATAAAAAAAACATTAAGTTGTAGATTTGTTTATTTAGAAATTGTGTCCTTCCATTTCTTTCACTTTCAACATGCTTGGAGACAGCTTTAATGGGTGGACTTTTGTGGGGAAGGGAAATCAAACCTGCTTTTATGGGGACTTTTGCAGATGAACTGCTCGTTCAATGTTCTTTTGTCTATAGTGGTTTGAATTTTAAATTTTGGGGTTTTATTGGTATGCACATTGCTGCATGTTTTACTCACTTTTGTTTTCTCACTAAGAATTTGTTCAAATCGTGCACAACTTTAGGCATGAAAACGAGCTGCTTTCTATTGATTGGTACGTGGAAGATTTCTTCTGCTTGCTGACTGGAAGTTTCCGACTGCTTTATGCTGTTCTAGTTTGTCATGGGATGCTATCATTCAAAGAAACCTAAATCTCAAGGGTACGAGGATCCCACTGTTCTTGCTGCAGAAACACCTTGTAAGTATCAAAATAAGCCTCTTAATTTTTTTTTCTACTTTTATCTAATGAATAGAACTTCTCTGCCCCTGCCATGAAACGATCTTCATTTTTCAATGGGATTGCTTCAGTATTTGTGCCTATTGGAGTGCCTTGATTGTTATCTGGTGTCTCGAATGCTCCAAAAACTTTTATTGTTTATAGATTATATCTAATTTACGATTTAGAACCCAGGATTTATAGAGTGTTATATATAGACCCTCTAATCTCAGTGGTGCAACTGTATCGGTAATTTTTTTCCCCTCAAATGATAAAATTGCTCTCTTGCTCTGCGTGGGGATATAGCTAACACGTTGTTAATAAACTTCGTAAATCTCGTGTCGTTCTTTACTGTTTAGAGTCTTGGACTTTTCATTTTGTTTCCACCATATAAAAGTGTCATAAGGAATTCTTTTAGTTATGTTTGAATTTTGAGTGTTTTCACTGAGTACTACCCTTCTGTATTCTATTTTAGAACCAGAGTATCAAGAAATTCATTCATTCATAATGAACATGGTCCATCTCTGTTAATCTTGGATAGCTTTTGTTCACCCCAAGGCGCTCCATTCATAGGGAGGTCCTCAAGGACTTCCTTTCAGGTTCACAAAAATTTGTAGATTTAAAATTGTGTTTTATTTGAAGGTAGATAAGATAGAAAGTGTATTACAACAATTCCGCCAGGAAGCTTAGTACCTCAGTTAAAACATCCCACATTTCAACTTTAATCCTCGTCGACTAATGATTTCAAATACGGACTATACGGCTTGCCAAAGGCTAGTTTATAAAATATGGTGGGCATTGTCAAATTTTTCTGTATTTTGGATGCAGTTACAGTGAGCGAGGTAGAGGCATTGTATGAGCTTTTCAAGAAGCTGAGCAGCTCAATTATTAATGATGGGTTGATCCATAGGGTACGCTGTATTAGCCAATTGTTTACTAGTTATTTCTAATTCTTTCCTTTCCAAAAGATTTAAAAATTGAGGCAAATACCAATATAAAACCTTGAGTTGGCTTTGTTTATCATTGGTGGTTGTATTATTGGCATTCTATACTAACATATATGTATCGAATGCACAGGAAGAATTTCAGCTTGCATTGTTCAGAAATAGAAATATGAAAAACCTATTTGCAGATAGAGTATGTTGCTGCCTTCTTTAACACCCTCTATTCTTCAAATCTTCATTTACCTCTGCCTTTTGCCTTAGTTACTGAAGAAACTATGGATTTTTTTTCCTTAAATGTAGATTTTTGATTTATTTGACGTGAAGCGCAATGGGGTTATTGAATTTGGGGAGTTTGTCAGATCATTAGGCATCTTCCATCCTAATGCATCTATTAAAGACAAAATTACATGTGAGATTTATTGCTGAGCTTTTGTACTGTACATCACCTATTTTCGTTAGTTATGTTCTTAAAACAATGACTTGCTGATCATTATGTTTTAATTTGCAGTTGCTTTTAGGCTATATGATTTGAGGCAGACAGGGTTTATAGAGCAAGAAGAGGTGACCTTTATTACTTATTTTTAGTTTATCTATGCAGAATCCTTCTGTTTAAAGCAATAATTTCTGTTAGCCATTTTCTGTTGGATCATCTTCAACAACGTTTTGCTTAGTCTTACCTATCATTTCGTTATGTTAAATGATATAATATTAAATTGGTAACTTCACCTATCAACTTAAACTTTTGGGTCAATCAATAATTTAAAATAATATTAGACCAAGTGGTTCAAAAAGTATTGTGTTCAAACCGTAGGCACGGGCAAATTTAGTATAGGCTCGAGGGGCTCGACTCCGCCCTCAACTTTATTCTCTTTATATAAGATGCATATAAAAAATTGATCTAATACCATTATTAGAAGTTGGCGTAATGGTAAAACTAGCTCTCATCTCCTTCTCAACTTTGGTTCAAGTCTCACTATTTACATTTTTTTTCAGATTATTTTTATTAAACTATAAAGCTCTCCGTCAACAAAATTTCTGGATCCGTCACTAACAATGGATTATTTCTTCTCCAATTATTATTGATTTCCACTTGTTGGGTCTTCTATATTTTTTAAATCCCAGAGTGAGACAAACATTGTGCATGGCTAACTAATGAAATTTGGACTAAACTTCAACTTCAACAGGATATGTACAACACTACACATACCCCTCCAACTTATGGTCTCATTTTTTGTTCTCTCAGTTGAAGGAAATGGTTTTGGCACTTCTTCATGAATCGGAGTTGACTCTTTCAGATGATGTCATTGAAACAATTGTGGAAAAGGTCTTCACCCAATGCTGTACTGCCTCTCATACTTATCCTGTTTTATTTAGAGAAAAAAATCATCATCTCTAATTCTGGTCTTCTACTGCAGACATTTAATGAAGCAGACATGAACGGTGATGGAAAGATTGACCAAGAAGAGTGGAGGGAGTTTGTGATGAAGTATCCATCATTGATCAAGAACATGACATTACCATATCTAAAGTAAGTAACGATGAGTTTGTTTGTTTAGCCATTATGCTCTCTTGCAATTCTTTAACACCACTTCCATGGATTCAGGGATATAACCATGGCATTCCCAAGTTTTGTTGCAAGTACAGAAGTTGAAGATTCGGAGATTTAGTAGACTTTCATCAATCCAACAAGTTTCTGTGGTTCAACAAGAATACCTACAACGACAATCGAGAGATACAGAGATTGCTCCCCCTAAAGAAGGCCTGTCAATTGGTGAACTCTGTGGTCATTTCACTCCAATAGTCCATCTGTTTTCCAGAAGCCAATTGCAAACAGAATTGTCGGTCGTTCGGGCTCAATCCAAATGATGTGGTTTTGCAGGAATAGAATCAGGTATTCTCTAGAATGTATGTTGGCAAGCCAAACCCCCACGTTTGGGAAGACCTGAGTTATTCATTGTAGATAATCTCTAGAGTTTCATTATGTGCATACAAATGTAGAAACTT

Coding sequence (CDS)

ATGGGATGCTATCATTCAAAGAAACCTAAATCTCAAGGGTACGAGGATCCCACTGTTCTTGCTGCAGAAACACCTTTTACAGTGAGCGAGGTAGAGGCATTGTATGAGCTTTTCAAGAAGCTGAGCAGCTCAATTATTAATGATGGGTTGATCCATAGGGAAGAATTTCAGCTTGCATTGTTCAGAAATAGAAATATGAAAAACCTATTTGCAGATAGAATTTTTGATTTATTTGACGTGAAGCGCAATGGGGTTATTGAATTTGGGGAGTTTGTCAGATCATTAGGCATCTTCCATCCTAATGCATCTATTAAAGACAAAATTACATTTGCTTTTAGGCTATATGATTTGAGGCAGACAGGGTTTATAGAGCAAGAAGAGTTGAAGGAAATGGTTTTGGCACTTCTTCATGAATCGGAGTTGACTCTTTCAGATGATGTCATTGAAACAATTGTGGAAAAGACATTTAATGAAGCAGACATGAACGGTGATGGAAAGATTGACCAAGAAGAGTGGAGGGAGTTTGTGATGAAGTATCCATCATTGATCAAGAACATGACATTACCATATCTAAAGTAA

Protein sequence

MGCYHSKKPKSQGYEDPTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLALFRNRNMKNLFADRIFDLFDVKRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQTGFIEQEELKEMVLALLHESELTLSDDVIETIVEKTFNEADMNGDGKIDQEEWREFVMKYPSLIKNMTLPYLK
Homology
BLAST of Cucsat.G15606.T10 vs. ExPASy Swiss-Prot
Match: O81223 (Calcineurin B-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=CBL4 PE=1 SV=3)

HSP 1 Score: 293.1 bits (749), Expect = 2.3e-78
Identity = 147/197 (74.62%), Postives = 169/197 (85.79%), Query Frame = 0

Query: 1   MGCYHSKKPKSQ-----GYEDPTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREE 60
           MGC  SKK K       GYEDP +LA+ TPFTV EVEALYELFKKLSSSII+DGLIH+EE
Sbjct: 1   MGCSVSKKKKKNAMRPPGYEDPELLASVTPFTVEEVEALYELFKKLSSSIIDDGLIHKEE 60

Query: 61  FQLALFRNRNMKNLFADRIFDLFDVKRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLY 120
           FQLALFRNRN +NLFADRIFD+FDVKRNGVIEFGEFVRSLG+FHP+A + +K+ FAF+LY
Sbjct: 61  FQLALFRNRNRRNLFADRIFDVFDVKRNGVIEFGEFVRSLGVFHPSAPVHEKVKFAFKLY 120

Query: 121 DLRQTGFIEQEELKEMVLALLHESELTLSDDVIETIVEKTFNEADMNGDGKIDQEEWREF 180
           DLRQTGFIE+EELKEMV+ALLHESEL LS+D+IE +V+K F +AD   DGKID +EW++F
Sbjct: 121 DLRQTGFIEREELKEMVVALLHESELVLSEDMIEVMVDKAFVQADRKNDGKIDIDEWKDF 180

Query: 181 VMKYPSLIKNMTLPYLK 193
           V   PSLIKNMTLPYLK
Sbjct: 181 VSLNPSLIKNMTLPYLK 197

BLAST of Cucsat.G15606.T10 vs. ExPASy Swiss-Prot
Match: Q3HRP0 (Calcineurin B-like protein 7 OS=Oryza sativa subsp. japonica OX=39947 GN=CBL7 PE=2 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 1.4e-72
Identity = 135/193 (69.95%), Postives = 161/193 (83.42%), Query Frame = 0

Query: 1   MGCYHSKK-PKSQGYEDPTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLA 60
           MGC  SK+  ++  +EDP +LA ET F+VSEVEALYELFKK+S SI  DGLIH+EEFQLA
Sbjct: 1   MGCISSKQFKRAAEHEDPAILAKETTFSVSEVEALYELFKKISHSIFKDGLIHKEEFQLA 60

Query: 61  LFRNRNMKNLFADRIFDLFDVKRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQ 120
           LFRN N KNLFADRIFDLFD+KRNGVI+FGEFVRSL IFHP   + +KI FAFRLYDLR 
Sbjct: 61  LFRNSNKKNLFADRIFDLFDLKRNGVIDFGEFVRSLNIFHPETPLAEKIAFAFRLYDLRG 120

Query: 121 TGFIEQEELKEMVLALLHESELTLSDDVIETIVEKTFNEADMNGDGKIDQEEWREFVMKY 180
           TG+IE+EEL EMVLA+L+ES+L LSDD +E IV++TF +AD+N DGKID +EW+ F  K 
Sbjct: 121 TGYIEREELYEMVLAILNESDLLLSDDAVEQIVDQTFKQADLNSDGKIDPDEWKAFASKN 180

Query: 181 PSLIKNMTLPYLK 193
           P+L+KNMTLPYLK
Sbjct: 181 PALLKNMTLPYLK 193

BLAST of Cucsat.G15606.T10 vs. ExPASy Swiss-Prot
Match: Q3HRN9 (Calcineurin B-like protein 8 OS=Oryza sativa subsp. japonica OX=39947 GN=CBL8 PE=2 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 1.7e-70
Identity = 133/193 (68.91%), Postives = 160/193 (82.90%), Query Frame = 0

Query: 1   MGCYHSKK-PKSQGYEDPTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLA 60
           MGC  SK+  ++  +EDP +LA ET F+VSEVEAL+ELFKK+S SI  DGLIH+EEFQLA
Sbjct: 1   MGCVSSKQFKRAAQHEDPAILAKETTFSVSEVEALFELFKKISHSIFRDGLIHKEEFQLA 60

Query: 61  LFRNRNMKNLFADRIFDLFDVKRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQ 120
           LFRN N KNLFA+RIFDLFD+KRNGVI+FGEFVRSL IFHP   + DKI FAFRLYDLR 
Sbjct: 61  LFRNSNKKNLFANRIFDLFDLKRNGVIDFGEFVRSLSIFHPETPLGDKIAFAFRLYDLRG 120

Query: 121 TGFIEQEELKEMVLALLHESELTLSDDVIETIVEKTFNEADMNGDGKIDQEEWREFVMKY 180
           TG IE+EEL EMVLALL+ES+L LS++ +E IV++TF +AD+N DGKID +EW+ F  K 
Sbjct: 121 TGCIEREELHEMVLALLNESDLFLSEEAVEQIVDQTFKQADLNDDGKIDPDEWKTFASKN 180

Query: 181 PSLIKNMTLPYLK 193
           P+L+KNMTLPYLK
Sbjct: 181 PALLKNMTLPYLK 193

BLAST of Cucsat.G15606.T10 vs. ExPASy Swiss-Prot
Match: O81445 (Calcineurin B-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=CBL1 PE=1 SV=3)

HSP 1 Score: 260.4 bits (664), Expect = 1.6e-68
Identity = 124/193 (64.25%), Postives = 166/193 (86.01%), Query Frame = 0

Query: 1   MGCYHSKKPKS-QGYEDPTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLA 60
           MGC+HSK  K  +G+EDP  LA+ET F+VSEVEAL+ELFK +SSS+++DGLI++EEFQLA
Sbjct: 1   MGCFHSKAAKEFRGHEDPVKLASETAFSVSEVEALFELFKSISSSVVDDGLINKEEFQLA 60

Query: 61  LFRNRNMKNLFADRIFDLFDVKRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQ 120
           LF++R  +N+FA+RIFD+FDVKR GVI+FG+FVRSL +FHPNAS++DKI F FRLYD+  
Sbjct: 61  LFKSRKRENIFANRIFDMFDVKRKGVIDFGDFVRSLNVFHPNASLEDKIDFTFRLYDMDC 120

Query: 121 TGFIEQEELKEMVLALLHESELTLSDDVIETIVEKTFNEADMNGDGKIDQEEWREFVMKY 180
           TG+IE++E+K+M++ALL ESE+ L+D+ IE I++KTF +AD+N DGKID+ EW +FV K 
Sbjct: 121 TGYIERQEVKQMLIALLCESEMKLADETIEIILDKTFEDADVNQDGKIDKLEWSDFVNKN 180

Query: 181 PSLIKNMTLPYLK 193
           PSL+K MTLPYL+
Sbjct: 181 PSLLKIMTLPYLR 193

BLAST of Cucsat.G15606.T10 vs. ExPASy Swiss-Prot
Match: Q75KU4 (Calcineurin B-like protein 4 OS=Oryza sativa subsp. japonica OX=39947 GN=CBL4 PE=1 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 1.8e-67
Identity = 128/193 (66.32%), Postives = 156/193 (80.83%), Query Frame = 0

Query: 1   MGCYHSKK-PKSQGYEDPTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLA 60
           MGC  SK+  +  GYE+P VLAA+T FTV+EVEAL EL+ K+S SII DGLIH+EEFQLA
Sbjct: 1   MGCASSKQFKRPPGYEEPAVLAAQTTFTVNEVEALRELYNKMSYSIIKDGLIHKEEFQLA 60

Query: 61  LFRNRNMKNLFADRIFDLFDVKRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQ 120
           LFRN    NLFADR+FDLFD+KRNGVIEFGEFVRSL +FHP A   +K  FAF+LYDLR 
Sbjct: 61  LFRNSRKANLFADRVFDLFDLKRNGVIEFGEFVRSLSVFHPKAPKSEKTAFAFKLYDLRG 120

Query: 121 TGFIEQEELKEMVLALLHESELTLSDDVIETIVEKTFNEADMNGDGKIDQEEWREFVMKY 180
           TG+IE+EEL+EMVLALL ES+L LS+  +E IV+ TF++AD NGDG+ID EEW EFV   
Sbjct: 121 TGYIEKEELREMVLALLDESDLHLSECAVEAIVDNTFSQADSNGDGRIDPEEWEEFVKAN 180

Query: 181 PSLIKNMTLPYLK 193
           P+ ++NM+LPYL+
Sbjct: 181 PASLRNMSLPYLQ 193

BLAST of Cucsat.G15606.T10 vs. NCBI nr
Match: XP_004146220.1 (calcineurin B-like protein 4 [Cucumis sativus] >XP_031737656.1 calcineurin B-like protein 4 [Cucumis sativus] >KAE8650550.1 hypothetical protein Csa_010195 [Cucumis sativus])

HSP 1 Score: 381 bits (979), Expect = 4.24e-133
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 0

Query: 1   MGCYHSKKPKSQGYEDPTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLAL 60
           MGCYHSKKPKSQGYEDPTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLAL
Sbjct: 1   MGCYHSKKPKSQGYEDPTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLAL 60

Query: 61  FRNRNMKNLFADRIFDLFDVKRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQT 120
           FRNRNMKNLFADRIFDLFDVKRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQT
Sbjct: 61  FRNRNMKNLFADRIFDLFDVKRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQT 120

Query: 121 GFIEQEELKEMVLALLHESELTLSDDVIETIVEKTFNEADMNGDGKIDQEEWREFVMKYP 180
           GFIEQEELKEMVLALLHESELTLSDDVIETIVEKTFNEADMNGDGKIDQEEWREFVMKYP
Sbjct: 121 GFIEQEELKEMVLALLHESELTLSDDVIETIVEKTFNEADMNGDGKIDQEEWREFVMKYP 180

Query: 181 SLIKNMTLPYLK 192
           SLIKNMTLPYLK
Sbjct: 181 SLIKNMTLPYLK 192

BLAST of Cucsat.G15606.T10 vs. NCBI nr
Match: XP_008466818.1 (PREDICTED: calcineurin B-like protein 4 isoform X1 [Cucumis melo] >XP_008466819.1 PREDICTED: calcineurin B-like protein 4 isoform X1 [Cucumis melo] >XP_008466821.1 PREDICTED: calcineurin B-like protein 4 isoform X1 [Cucumis melo] >KAA0050504.1 calcineurin B-like protein 4 isoform X1 [Cucumis melo var. makuwa] >TYK29181.1 calcineurin B-like protein 4 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 378 bits (970), Expect = 1.24e-131
Identity = 190/192 (98.96%), Postives = 191/192 (99.48%), Query Frame = 0

Query: 1   MGCYHSKKPKSQGYEDPTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLAL 60
           MGCYHSKKPK+QGYEDPTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLAL
Sbjct: 7   MGCYHSKKPKTQGYEDPTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLAL 66

Query: 61  FRNRNMKNLFADRIFDLFDVKRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQT 120
           FRNRNMKNLFADRIFDLFDVKRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQT
Sbjct: 67  FRNRNMKNLFADRIFDLFDVKRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQT 126

Query: 121 GFIEQEELKEMVLALLHESELTLSDDVIETIVEKTFNEADMNGDGKIDQEEWREFVMKYP 180
           GFIEQEELKEMVLALLHESELTLSDDVIETIVEKTFNEAD NGDGKIDQEEWREFVMKYP
Sbjct: 127 GFIEQEELKEMVLALLHESELTLSDDVIETIVEKTFNEADTNGDGKIDQEEWREFVMKYP 186

Query: 181 SLIKNMTLPYLK 192
           SLIKNMTLPYLK
Sbjct: 187 SLIKNMTLPYLK 198

BLAST of Cucsat.G15606.T10 vs. NCBI nr
Match: XP_038881280.1 (calcineurin B-like protein 4 [Benincasa hispida] >XP_038881288.1 calcineurin B-like protein 4 [Benincasa hispida])

HSP 1 Score: 377 bits (967), Expect = 2.86e-131
Identity = 190/192 (98.96%), Postives = 190/192 (98.96%), Query Frame = 0

Query: 1   MGCYHSKKPKSQGYEDPTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLAL 60
           MGCYHSKKPKSQGYEDP VLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLAL
Sbjct: 1   MGCYHSKKPKSQGYEDPIVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLAL 60

Query: 61  FRNRNMKNLFADRIFDLFDVKRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQT 120
           FRNRNMKNLFADRIFDLFDVKRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQT
Sbjct: 61  FRNRNMKNLFADRIFDLFDVKRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQT 120

Query: 121 GFIEQEELKEMVLALLHESELTLSDDVIETIVEKTFNEADMNGDGKIDQEEWREFVMKYP 180
           GFIEQEELKEMVLALLHESEL LSDDVIETIVEKTFNEADMNGDGKIDQEEWREFVMKYP
Sbjct: 121 GFIEQEELKEMVLALLHESELILSDDVIETIVEKTFNEADMNGDGKIDQEEWREFVMKYP 180

Query: 181 SLIKNMTLPYLK 192
           SLIKNMTLPYLK
Sbjct: 181 SLIKNMTLPYLK 192

BLAST of Cucsat.G15606.T10 vs. NCBI nr
Match: KAG6576654.1 (Calcineurin B-like protein 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014705.1 Calcineurin B-like protein 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 370 bits (951), Expect = 7.87e-129
Identity = 186/192 (96.88%), Postives = 189/192 (98.44%), Query Frame = 0

Query: 1   MGCYHSKKPKSQGYEDPTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLAL 60
           MGCYHSKKPKS GYE+PTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLAL
Sbjct: 1   MGCYHSKKPKSPGYEEPTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLAL 60

Query: 61  FRNRNMKNLFADRIFDLFDVKRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQT 120
           FRNRNMKNLFADRIFDLFD+KRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQT
Sbjct: 61  FRNRNMKNLFADRIFDLFDLKRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQT 120

Query: 121 GFIEQEELKEMVLALLHESELTLSDDVIETIVEKTFNEADMNGDGKIDQEEWREFVMKYP 180
           GFIEQEELKEMVLALLHESEL LSDDVIETIVEKTFNEADM GDGKID+EEWREFVMKYP
Sbjct: 121 GFIEQEELKEMVLALLHESELILSDDVIETIVEKTFNEADMKGDGKIDEEEWREFVMKYP 180

Query: 181 SLIKNMTLPYLK 192
           SLIKNMTLPYLK
Sbjct: 181 SLIKNMTLPYLK 192

BLAST of Cucsat.G15606.T10 vs. NCBI nr
Match: XP_023553007.1 (calcineurin B-like protein 4 [Cucurbita pepo subsp. pepo] >XP_023553008.1 calcineurin B-like protein 4 [Cucurbita pepo subsp. pepo] >XP_023553009.1 calcineurin B-like protein 4 [Cucurbita pepo subsp. pepo] >XP_023553010.1 calcineurin B-like protein 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 370 bits (951), Expect = 7.87e-129
Identity = 186/192 (96.88%), Postives = 189/192 (98.44%), Query Frame = 0

Query: 1   MGCYHSKKPKSQGYEDPTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLAL 60
           MGCYHSKKPKS GYE+PTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLAL
Sbjct: 1   MGCYHSKKPKSPGYEEPTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLAL 60

Query: 61  FRNRNMKNLFADRIFDLFDVKRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQT 120
           FRNRNMKNLFADRIFDLFD+KRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQT
Sbjct: 61  FRNRNMKNLFADRIFDLFDLKRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQT 120

Query: 121 GFIEQEELKEMVLALLHESELTLSDDVIETIVEKTFNEADMNGDGKIDQEEWREFVMKYP 180
           GFIEQEELKEMVLALLHESEL LSDDVIETIVEKTFNEADM GDGKID+EEWREFVMKYP
Sbjct: 121 GFIEQEELKEMVLALLHESELILSDDVIETIVEKTFNEADMKGDGKIDEEEWREFVMKYP 180

Query: 181 SLIKNMTLPYLK 192
           SLIKNMTLPYLK
Sbjct: 181 SLIKNMTLPYLK 192

BLAST of Cucsat.G15606.T10 vs. ExPASy TrEMBL
Match: A0A1S3CTE6 (calcineurin B-like protein 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103504134 PE=4 SV=1)

HSP 1 Score: 378 bits (970), Expect = 5.98e-132
Identity = 190/192 (98.96%), Postives = 191/192 (99.48%), Query Frame = 0

Query: 1   MGCYHSKKPKSQGYEDPTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLAL 60
           MGCYHSKKPK+QGYEDPTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLAL
Sbjct: 7   MGCYHSKKPKTQGYEDPTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLAL 66

Query: 61  FRNRNMKNLFADRIFDLFDVKRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQT 120
           FRNRNMKNLFADRIFDLFDVKRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQT
Sbjct: 67  FRNRNMKNLFADRIFDLFDVKRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQT 126

Query: 121 GFIEQEELKEMVLALLHESELTLSDDVIETIVEKTFNEADMNGDGKIDQEEWREFVMKYP 180
           GFIEQEELKEMVLALLHESELTLSDDVIETIVEKTFNEAD NGDGKIDQEEWREFVMKYP
Sbjct: 127 GFIEQEELKEMVLALLHESELTLSDDVIETIVEKTFNEADTNGDGKIDQEEWREFVMKYP 186

Query: 181 SLIKNMTLPYLK 192
           SLIKNMTLPYLK
Sbjct: 187 SLIKNMTLPYLK 198

BLAST of Cucsat.G15606.T10 vs. ExPASy TrEMBL
Match: A0A5A7U5I9 (Calcineurin B-like protein 4 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold120G003710 PE=4 SV=1)

HSP 1 Score: 378 bits (970), Expect = 5.98e-132
Identity = 190/192 (98.96%), Postives = 191/192 (99.48%), Query Frame = 0

Query: 1   MGCYHSKKPKSQGYEDPTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLAL 60
           MGCYHSKKPK+QGYEDPTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLAL
Sbjct: 7   MGCYHSKKPKTQGYEDPTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLAL 66

Query: 61  FRNRNMKNLFADRIFDLFDVKRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQT 120
           FRNRNMKNLFADRIFDLFDVKRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQT
Sbjct: 67  FRNRNMKNLFADRIFDLFDVKRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQT 126

Query: 121 GFIEQEELKEMVLALLHESELTLSDDVIETIVEKTFNEADMNGDGKIDQEEWREFVMKYP 180
           GFIEQEELKEMVLALLHESELTLSDDVIETIVEKTFNEAD NGDGKIDQEEWREFVMKYP
Sbjct: 127 GFIEQEELKEMVLALLHESELTLSDDVIETIVEKTFNEADTNGDGKIDQEEWREFVMKYP 186

Query: 181 SLIKNMTLPYLK 192
           SLIKNMTLPYLK
Sbjct: 187 SLIKNMTLPYLK 198

BLAST of Cucsat.G15606.T10 vs. ExPASy TrEMBL
Match: A0A6J1JB34 (calcineurin B-like protein 4 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111482857 PE=4 SV=1)

HSP 1 Score: 366 bits (939), Expect = 2.57e-127
Identity = 183/192 (95.31%), Postives = 189/192 (98.44%), Query Frame = 0

Query: 1   MGCYHSKKPKSQGYEDPTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLAL 60
           MGCY+SKKPKS GYE+PTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLAL
Sbjct: 1   MGCYYSKKPKSPGYEEPTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLAL 60

Query: 61  FRNRNMKNLFADRIFDLFDVKRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQT 120
           FRNRNMKNLF+DRIFDLFD+KRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQT
Sbjct: 61  FRNRNMKNLFSDRIFDLFDLKRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQT 120

Query: 121 GFIEQEELKEMVLALLHESELTLSDDVIETIVEKTFNEADMNGDGKIDQEEWREFVMKYP 180
           GFIEQ+ELKEMVLALLHESEL LSDDVIETIVEKTFNEADM GDGKID+EEWREFVMKYP
Sbjct: 121 GFIEQQELKEMVLALLHESELILSDDVIETIVEKTFNEADMKGDGKIDEEEWREFVMKYP 180

Query: 181 SLIKNMTLPYLK 192
           SLIKNMTLPYLK
Sbjct: 181 SLIKNMTLPYLK 192

BLAST of Cucsat.G15606.T10 vs. ExPASy TrEMBL
Match: A0A6J1CJH4 (calcineurin B-like protein 4 OS=Momordica charantia OX=3673 GN=LOC111011566 PE=4 SV=1)

HSP 1 Score: 359 bits (922), Expect = 1.00e-124
Identity = 180/192 (93.75%), Postives = 188/192 (97.92%), Query Frame = 0

Query: 1   MGCYHSKKPKSQGYEDPTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLAL 60
           MGCYHSKK K+ GYE+PTVLAAETPFTVSEVEAL+ELFKKLSSSII+DGLIHREEFQLAL
Sbjct: 1   MGCYHSKKSKTPGYEEPTVLAAETPFTVSEVEALFELFKKLSSSIIDDGLIHREEFQLAL 60

Query: 61  FRNRNMKNLFADRIFDLFDVKRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQT 120
           FRN+NM+NLFADRIFDLFD+KRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQT
Sbjct: 61  FRNQNMRNLFADRIFDLFDLKRNGVIEFGEFVRSLGIFHPNASIKDKITFAFRLYDLRQT 120

Query: 121 GFIEQEELKEMVLALLHESELTLSDDVIETIVEKTFNEADMNGDGKIDQEEWREFVMKYP 180
           GFIEQEELKEMVLALLHESEL LSDDVIETIVEKTFNEAD NGDGKIDQEEWREFVMK+P
Sbjct: 121 GFIEQEELKEMVLALLHESELILSDDVIETIVEKTFNEADANGDGKIDQEEWREFVMKHP 180

Query: 181 SLIKNMTLPYLK 192
           SLIKNMTLPYLK
Sbjct: 181 SLIKNMTLPYLK 192

BLAST of Cucsat.G15606.T10 vs. ExPASy TrEMBL
Match: A0A6J1J942 (calcineurin B-like protein 4 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111482857 PE=4 SV=1)

HSP 1 Score: 355 bits (910), Expect = 4.92e-123
Identity = 182/203 (89.66%), Postives = 188/203 (92.61%), Query Frame = 0

Query: 1   MGCYHSKKPKSQGYEDPTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLAL 60
           MGCY+SKKPKS GYE+PTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLAL
Sbjct: 1   MGCYYSKKPKSPGYEEPTVLAAETPFTVSEVEALYELFKKLSSSIINDGLIHREEFQLAL 60

Query: 61  FRNRNMKNLFADRIFDLFDVKRNGVIEFGEFVRSLGIFHPNASIKDKIT----------- 120
           FRNRNMKNLF+DRIFDLFD+KRNGVIEFGEFVRSLGIFHPNASIKDKIT           
Sbjct: 61  FRNRNMKNLFSDRIFDLFDLKRNGVIEFGEFVRSLGIFHPNASIKDKITCESLFQIFLFS 120

Query: 121 FAFRLYDLRQTGFIEQEELKEMVLALLHESELTLSDDVIETIVEKTFNEADMNGDGKIDQ 180
            AFRLYDLRQTGFIEQ+ELKEMVLALLHESEL LSDDVIETIVEKTFNEADM GDGKID+
Sbjct: 121 VAFRLYDLRQTGFIEQQELKEMVLALLHESELILSDDVIETIVEKTFNEADMKGDGKIDE 180

Query: 181 EEWREFVMKYPSLIKNMTLPYLK 192
           EEWREFVMKYPSLIKNMTLPYLK
Sbjct: 181 EEWREFVMKYPSLIKNMTLPYLK 203

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O812232.3e-7874.62Calcineurin B-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=CBL4 PE=1 SV=3[more]
Q3HRP01.4e-7269.95Calcineurin B-like protein 7 OS=Oryza sativa subsp. japonica OX=39947 GN=CBL7 PE... [more]
Q3HRN91.7e-7068.91Calcineurin B-like protein 8 OS=Oryza sativa subsp. japonica OX=39947 GN=CBL8 PE... [more]
O814451.6e-6864.25Calcineurin B-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=CBL1 PE=1 SV=3[more]
Q75KU41.8e-6766.32Calcineurin B-like protein 4 OS=Oryza sativa subsp. japonica OX=39947 GN=CBL4 PE... [more]
Match NameE-valueIdentityDescription
XP_004146220.14.24e-133100.00calcineurin B-like protein 4 [Cucumis sativus] >XP_031737656.1 calcineurin B-lik... [more]
XP_008466818.11.24e-13198.96PREDICTED: calcineurin B-like protein 4 isoform X1 [Cucumis melo] >XP_008466819.... [more]
XP_038881280.12.86e-13198.96calcineurin B-like protein 4 [Benincasa hispida] >XP_038881288.1 calcineurin B-l... [more]
KAG6576654.17.87e-12996.88Calcineurin B-like protein 4, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_023553007.17.87e-12996.88calcineurin B-like protein 4 [Cucurbita pepo subsp. pepo] >XP_023553008.1 calcin... [more]
Match NameE-valueIdentityDescription
A0A1S3CTE65.98e-13298.96calcineurin B-like protein 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103504134 ... [more]
A0A5A7U5I95.98e-13298.96Calcineurin B-like protein 4 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A6J1JB342.57e-12795.31calcineurin B-like protein 4 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111482... [more]
A0A6J1CJH41.00e-12493.75calcineurin B-like protein 4 OS=Momordica charantia OX=3673 GN=LOC111011566 PE=4... [more]
A0A6J1J9424.92e-12389.66calcineurin B-like protein 4 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111482... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00450RECOVERINcoord: 167..187
score: 19.96
coord: 74..95
score: 40.73
coord: 121..139
score: 28.95
NoneNo IPR availableGENE3D1.10.238.10coord: 16..192
e-value: 7.3E-62
score: 210.2
NoneNo IPR availablePANTHERPTHR23056CALCINEURIN Bcoord: 1..191
NoneNo IPR availablePANTHERPTHR23056:SF123CALCINEURIN B-LIKE PROTEIN 4coord: 1..191
IPR002048EF-hand domainSMARTSM00054efh_1coord: 151..179
e-value: 3.5E-4
score: 29.9
coord: 70..98
e-value: 6.1
score: 11.4
coord: 107..135
e-value: 2.2
score: 15.0
IPR002048EF-hand domainPFAMPF13833EF-hand_8coord: 48..93
e-value: 0.0053
score: 16.5
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 106..176
e-value: 2.1E-10
score: 40.9
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 147..182
score: 12.672309
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 103..138
score: 11.38912
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 66..101
score: 10.998585
IPR002048EF-hand domainCDDcd00051EFhcoord: 107..177
e-value: 1.14568E-10
score: 52.9353
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 160..172
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 19..190

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G15606Cucsat.G15606gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G15606.T10.E1Cucsat.G15606.T10.E1exon
Cucsat.G15606.T10.E2Cucsat.G15606.T10.E2exon
Cucsat.G15606.T10.E3Cucsat.G15606.T10.E3exon
Cucsat.G15606.T10.E4Cucsat.G15606.T10.E4exon
Cucsat.G15606.T10.E5Cucsat.G15606.T10.E5exon
Cucsat.G15606.T10.E6Cucsat.G15606.T10.E6exon
Cucsat.G15606.T10.E7Cucsat.G15606.T10.E7exon
Cucsat.G15606.T10.E8Cucsat.G15606.T10.E8exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G15606.T10.C1Cucsat.G15606.T10.C1CDS
Cucsat.G15606.T10.C2Cucsat.G15606.T10.C2CDS
Cucsat.G15606.T10.C3Cucsat.G15606.T10.C3CDS
Cucsat.G15606.T10.C4Cucsat.G15606.T10.C4CDS
Cucsat.G15606.T10.C5Cucsat.G15606.T10.C5CDS
Cucsat.G15606.T10.C6Cucsat.G15606.T10.C6CDS
Cucsat.G15606.T10.C7Cucsat.G15606.T10.C7CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G15606.T10Cucsat.G15606.T10-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding