Cucsat.G15448.T6 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G15448.T6
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionV-type proton ATPase subunit E
Locationctg2009: 5429793 .. 5433817 (-)
RNA-Seq ExpressionCucsat.G15448.T6
SyntenyCucsat.G15448.T6
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGTCTCCCCGTGAAAGGAAAAAAGAAAATAGAAAGAAAGAAGAAAAAAAAGAAAAAAAAGAAAAAAACAATGGAAGGCTGGTTGATATGATATCTTGTTTGGGTTTGCGATCGTGTTCCAATCTCTCTCACGTTGCCAACGTTCAGCGGGGCCCCCTTCTTCCAAATCTCACAATCTCCCTTTCACTCTTTCTTTCTTTCTTCTCTCATCCTTTCTCAATACTTCTTCGTTTTTGGTCTCCTCTCTCGAGATCGGTACGAACTCTCTGCTCTCTCTCTCACGCTCTGCACTCTATCTACTGTTTTTTCTTCTCACACCGCCCAACGCTTTCGTTCTTTTTTTTTTTTTTTTTCTTTTTTCTTTTTTTTTTTTATCTATCTAGGCATCTTAGAGCAAACGATCACAGCAACGCCTGAGTTTCTAGGGCTTCGATCTTCACACACACTTCGCCAAAATGAATGACGCAGATGTTTCCAAGCAGATCCAGCAGATGGTCAGGTTCATCCGCCAGGAAGCCGAGGAAAAGGCCAACGAGATCTCCGTCTCTGCTGAAGAAGTATTTCTTTCATTTTTCCTTCGATTTCTACTTCTTTCTGCTTTAGATGCCATGATTTTACTTCTTACTTCTCTAATCCTCCTAAGAACAACCTACTTATCGGCCTTGCTTTATGATCGCTTACCTATGCCTTACAGATTCTAGGTTTCGTATATGTTGTATTTCTGTTTGTGTGCATTTACTACTGAACGGAAGGAATTCATTTTCTTTTTTCTTTCTATTGTAGGAATTCAATATAGAAAAGTTGCAGCTGGTTGAGGCAGAGAAGAAGAAGATCAGGCAAGAGTACGAGAAGAAGGAGAAGCAAGTTGAAATTCGGAAGAAGATGTAATTATCTGCACCTTTTTTCCTCCCTATAACTTGTTAATTATAGTTTCATTATTCATGCTTCATAGTTGCTTGGTTTGTTAGGGCGCACAATTTTCAATTGCTGTAAAGATTTCTTTGGTTTTTCTGCCTCAAAGTTTGTAATAGATGAATAACATCTTCGTGGAATTGATGTTTTATCCAGCGAGTACTCAATGCAGCTCAATGCTTCTCGTATCAAAGTTCTGCAAGCTCAGGATGATGTTGTAAACGACATGAAAGAGGCAGCATCAAAGGAGCTTCTCAGTATAGGCCAAAATGAACATGTCTACAAAAATCTTTTGAAGGATCTCATTGTTCAGGTTAGTAAATTTCAAATATTATCTATATTTGAATGTCATAAAGTTTGATGTATCCTTAATGATTCTTTGATTTAGTAAAACTTCTTAAAGTCATAAAGTTTTCTGCGTATGGGCTAGTTCTTATAGTTGTCTGTTTATTACACTTAAAGAGTTCATTGCAAGTGCTACTAATTGTTTATGCTAGATAATTAGGAATTTTAAATGTCCTAAACAAATCTTTGTTTCACATAGCTCTGGATGGATAAAATTCTCCTATCTGTAAGGTATCAAATTGAAACACTCATCATCCTGATAAACAATGATTGAGTTTTCTTTTTTTCATCTCTTTGTTGATGGTTGCTGCTTAGAAGGCATTGTGGGGGTTGATCTTCCCTTTAAATTTTGGTTAGTATGGTTGCTGTAGGATAAGTATGATGCTGGATATACATATACTAGATGTGTTGTGCACTGCCTAAAGGTTTTTTGTTATTTTATAGAGCTTGCTTCGACTGAAAGAGCCAGCTGTTTTATTGCGATGCCGCAAAAGTGACGTCTATTTGGTAGAGTCTGTGCTGGGTTCAGCAGCTGTTGAATATGCAGAGAAAGAAAAAGTTCATGAGCCAGAGATCATTGTTGACCATGTCCATCTTCCACCTGGTCCAAGCCATCACCATCAGCACGGTCCTTCTTGGTAATCTTTCCTTTTCGATCTTTACCATTTTTTGCATATGAACTTCCTGCTTCTTAAACTTGGTCAGTTATAATATTATTTCAGCATTGGCAAAGTTATGCTTATGTGTTATGAGATTTGGAGGTAACTTTTGAATACTTTGCCTCAGCCTGACAGGCATATCTTATTTTTCTAGTTTGTAACTAATTACACCTTCCCTGGGGACAGGCACAATTTTTTTTAGGATTATTAATCCTGCAATTTCTTACAATTGCAGATTGTACCTTTTTATTTTGACAATAGTCTTAGATACACCATGGTTCGTTCATTTCCAAATCTGTGAATTGAACTATGCTAATAAACAATACTGTTACTTTTGATAGAGATACTAGGCATGGCATTTTTTATGTTAATCCATGTTTACTAATTCAGTTAAGAAGTATCAGTTGTAATTGATAGAATGGTTCAATTTGTAGAATTTGCAAACTACGAGGGCTGTCAAATATGAACTTCAAAATACAAGGTTATGCTGTATGAAATTTGACCGGAGTACAATTTAAAATTTTCTCTATGTAAATTGTATTGAGCCCTTGTATCACAAGGTTTTCTCCTTTTGGTTCTCTATGCTATGCAGTATGCACTGTTTTCTATGAGAAATGAACAATATGTCTATAGGCTTCTAATATTAAGCTTTGATCGTTCAGCTCTGGAGGAGTGGTCTTGGCATCTCGAGATGGGAAGATTGTTTGTGAGAACACCCTTGATGCTAGATTGGATGTTGTGTTCCGTAAAAAACTTCCAGAGGTACGTATACTTCTCAACCATACCTTACTGTGTTACTGGGGTGGGTGCTAATTACATCACTAATTACTAATCCGCTCTAATTGTCTTACATCATTTTTAGAGCGAAAAAGTTCTTCAAAAAGAATTTTAGAAAGGAAAAGATCCACATTTAGAAGATTTCTTGTTTTGCTAGAAGACAAGGCTTAGTTTACTAGGTTGTTACTCAGTTTTTTTCTTTTAATTTTATAAATGTCCACTGCTATCGGTTGTGTATTCTCTACATACAAAGATGGAAAAGTTTTGTTGCACCATTCCAGAATTTGGATGAATCTTTCACAAATAACAGCACATACTTAAAGATTAAGGAGAGGATGTGAGACAAGCTTTAACGTTTTTGCCGACTGCATCTGTAAGTTCCTCAGTGCGTTATTATTTTACTGACGGGTCCACTCAAATTCATCTGCCCAGGATGCATATTTCTAACTTTGGTTAGTTACGCTTAGATTGGCAATAAAGAGGTCAAACTTTTAACTTTTGATTAACATTTAGGAATATAAGCTCAACAACATGATTCCTTTACTTTATATCGGCCTTCATAGGTGCACTTTTATACTCCTCCCTCACCTTTTCTTATCCTTTAGGAATATACAAATTCTTTATTCGAGGATTTTACACTTTTGATAAAAGAACAAAAATACAAATACTTTATCGGGGGCTCTTTGCAATATTCCAATACGTCTTTCTTTTATCACCCCTTTTCCAACTGCAAAGGAACGCCGATGGTTTTTTATTTTGTTGCACCATTTTATCAACGTTGTTCATATTTAATGTAATAAACTAACAGCTGTATTTCTCATTGGCAGATCCGTAAAAGCCTTTTCAGTCAAGTTGCTGCATGATGGATTGGATTCGTCTCTATCATGGCGCTTCCTGTGAAATAATTTATGAGTCCAAATTTGGTCTTTGATGCGAATTTTATGCCCCGAATGTCACGTATCAATAAGTATTACATTCTTTGATTTTCTTTATCTGATAGTTACAATGTTCTTGGTTTGAGCTCTACGGGGAGGTAGGCGCCCCTATCGTCTCGATAGAACCTCCGGTATCAATAATATTCCAGCTTGATAAATTGGTTTCCTTGTTCAAGTTGGGATGGCCTTTTTAGTTAAGTATATTTTTGTGTGCACATCATACAGACTATTGTCTTTATATAATGTAATGTTTGACATTTCTCTTCTGAAGGCAGTTGTTTCAAGTAAAGTTATTTCACTTGCATTCAGTTTTGTATAAGTCTTTCAATAAAATACAATGTGTTCATTCTCCTAGGTATGGTTAAGAAATGATTGTTTATCTTATTATTGACTTTTC

Coding sequence (CDS)

ATGAATGACGCAGATGTTTCCAAGCAGATCCAGCAGATGGTCAGGTTCATCCGCCAGGAAGCCGAGGAAAAGGCCAACGAGATCTCCGTCTCTGCTGAAGAAGAATTCAATATAGAAAAGTTGCAGCTGGTTGAGGCAGAGAAGAAGAAGATCAGGCAAGAGTACGAGAAGAAGGAGAAGCAAGTTGAAATTCGGAAGAAGATCGAGTACTCAATGCAGCTCAATGCTTCTCGTATCAAAGTTCTGCAAGCTCAGGATGATGTTGTAAACGACATGAAAGAGGCAGCATCAAAGGAGCTTCTCAGTATAGGCCAAAATGAACATGTCTACAAAAATCTTTTGAAGGATCTCATTGTTCAGAGCTTGCTTCGACTGAAAGAGCCAGCTGTTTTATTGCGATGCCGCAAAAGTGACGTCTATTTGGTAGAGTCTGTGCTGGGTTCAGCAGCTGTTGAATATGCAGAGAAAGAAAAAGTTCATGAGCCAGAGATCATTGTTGACCATGTCCATCTTCCACCTGGTCCAAGCCATCACCATCAGCACGGTCCTTCTTGGTAA

Protein sequence

MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEKQVEIRKKIEYSMQLNASRIKVLQAQDDVVNDMKEAASKELLSIGQNEHVYKNLLKDLIVQSLLRLKEPAVLLRCRKSDVYLVESVLGSAAVEYAEKEKVHEPEIIVDHVHLPPGPSHHHQHGPSW
Homology
BLAST of Cucsat.G15448.T6 vs. ExPASy Swiss-Prot
Match: O23948 (V-type proton ATPase subunit E OS=Gossypium hirsutum OX=3635 GN=VATE PE=2 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 7.2e-74
Identity = 157/191 (82.20%), Postives = 166/191 (86.91%), Query Frame = 0

Query: 1   MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK 60
           M+DADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK
Sbjct: 1   MSDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK 60

Query: 61  QVEIRKKIEYSMQLNASRIKVLQAQDDVVNDMKEAASKELLSIGQNEHVYKNLLKDLIVQ 120
           QVEIRKKIEYSMQLNASRIKVLQAQDDVVN MKE+ASK+ L++  + HVYK LLKDLIVQ
Sbjct: 61  QVEIRKKIEYSMQLNASRIKVLQAQDDVVNAMKESASKDFLNVSHDHHVYKRLLKDLIVQ 120

Query: 121 SLLRLKEPAVLLRCRKSDVYLVESVLGSAAVEYAEKEKVHEPEIIVDHVHLPPGPSHHH- 180
           SL+RLKEP VLLRCRK D++LVESVL SA  EYA K  VH PEIIVD VHLPPGPSHHH 
Sbjct: 121 SLVRLKEPGVLLRCRKEDLHLVESVLDSAKEEYASKVNVHPPEIIVDDVHLPPGPSHHHG 180

Query: 181 -------QHGP 184
                   HGP
Sbjct: 181 FFHHHAEAHGP 191

BLAST of Cucsat.G15448.T6 vs. ExPASy Swiss-Prot
Match: Q9SWE7 (V-type proton ATPase subunit E OS=Citrus limon OX=2708 GN=VATE PE=2 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 4.7e-73
Identity = 154/185 (83.24%), Postives = 170/185 (91.89%), Query Frame = 0

Query: 1   MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK 60
           MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYE+KEK
Sbjct: 1   MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYERKEK 60

Query: 61  QVEIRKKIEYSMQLNASRIKVLQAQDDVVNDMKEAASKELLSIGQNEHVYKNLLKDLIVQ 120
           QVEIRKKIEYSMQLNASRIKVLQAQDD+V++M EAASKE+L++ ++ + YK LLKDLIVQ
Sbjct: 61  QVEIRKKIEYSMQLNASRIKVLQAQDDLVSNMMEAASKEVLNVSRDHNSYKKLLKDLIVQ 120

Query: 121 SLLRLKEPAVLLRCRKSDVYLVESVLGSAAVEYAEKEKVHEPEIIVD-HVHLPPGPSHHH 180
           SLLRLKEPAVLLRCRK D +LVESVL SA  EYA+K +VH PEIIVD H++LPPGP HH+
Sbjct: 121 SLLRLKEPAVLLRCRKDDHHLVESVLESAKEEYAQKLQVHPPEIIVDHHIYLPPGPGHHN 180

Query: 181 QHGPS 185
            HGPS
Sbjct: 181 AHGPS 185

BLAST of Cucsat.G15448.T6 vs. ExPASy Swiss-Prot
Match: Q9MB46 (V-type proton ATPase subunit E OS=Citrus unshiu OX=55188 GN=VATE PE=2 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 3.0e-72
Identity = 153/185 (82.70%), Postives = 169/185 (91.35%), Query Frame = 0

Query: 1   MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK 60
           MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYE+KEK
Sbjct: 1   MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYERKEK 60

Query: 61  QVEIRKKIEYSMQLNASRIKVLQAQDDVVNDMKEAASKELLSIGQNEHVYKNLLKDLIVQ 120
           QVEIRKKIEYSMQLNASRIKVLQAQDD+V++M EAASKE+L++ ++ + YK LLK LIVQ
Sbjct: 61  QVEIRKKIEYSMQLNASRIKVLQAQDDLVSNMMEAASKEVLNVSRDHNSYKKLLKGLIVQ 120

Query: 121 SLLRLKEPAVLLRCRKSDVYLVESVLGSAAVEYAEKEKVHEPEIIVD-HVHLPPGPSHHH 180
           SLLRLKEPAVLLRCRK D +LVESVL SA  EYA+K +VH PEIIVD H++LPPGP HH+
Sbjct: 121 SLLRLKEPAVLLRCRKDDHHLVESVLESAKEEYAQKLQVHPPEIIVDHHIYLPPGPGHHN 180

Query: 181 QHGPS 185
            HGPS
Sbjct: 181 AHGPS 185

BLAST of Cucsat.G15448.T6 vs. ExPASy Swiss-Prot
Match: Q41396 (V-type proton ATPase subunit E OS=Spinacia oleracea OX=3562 GN=VATE PE=2 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 1.4e-69
Identity = 143/182 (78.57%), Postives = 160/182 (87.91%), Query Frame = 0

Query: 1   MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK 60
           MND DV KQIQQMVRFIRQEAEEKANEISV+AEEEFNIEKLQLVEAEKKKIR EYE+KEK
Sbjct: 1   MNDTDVQKQIQQMVRFIRQEAEEKANEISVAAEEEFNIEKLQLVEAEKKKIRPEYERKEK 60

Query: 61  QVEIRKKIEYSMQLNASRIKVLQAQDDVVNDMKEAASKELLSIGQNEHVYKNLLKDLIVQ 120
           QV++R+KIEYSMQLNASRIKVLQAQDD+VN MKE A+KELL +  + H YK LLK+L+VQ
Sbjct: 61  QVQVRRKIEYSMQLNASRIKVLQAQDDLVNSMKEEAAKELLRVSGDHHHYKRLLKELVVQ 120

Query: 121 SLLRLKEPAVLLRCRKSDVYLVESVLGSAAVEYAEKEKVHEPEIIVDHVHLPPGPSHHHQ 180
           SLLRL+EP VLLRCR+ DV+LVE VL SA  EYAEK +VH PEIIVD +HLP GPSHH +
Sbjct: 121 SLLRLREPGVLLRCREDDVHLVEHVLNSAKEEYAEKAEVHTPEIIVDSIHLPAGPSHHKE 180

Query: 181 HG 183
           HG
Sbjct: 181 HG 182

BLAST of Cucsat.G15448.T6 vs. ExPASy Swiss-Prot
Match: Q40272 (V-type proton ATPase subunit E OS=Mesembryanthemum crystallinum OX=3544 GN=VATE PE=2 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 8.9e-64
Identity = 137/181 (75.69%), Postives = 153/181 (84.53%), Query Frame = 0

Query: 1   MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK 60
           MND DV  QIQQMVRF+RQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYE+K K
Sbjct: 1   MNDTDVQNQIQQMVRFMRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYERKAK 60

Query: 61  QVEIRKKIEYSMQLNASRIKVLQAQDDVVNDMKEAASKELLSIGQNEHVYKNLLKDLIVQ 120
           QV++R+KIEYSMQLNASRIKVLQAQDD+VN MKEAASKELL +  + H Y+NLLK+LIVQ
Sbjct: 61  QVDVRRKIEYSMQLNASRIKVLQAQDDLVNAMKEAASKELLLVSGDHHQYRNLLKELIVQ 120

Query: 121 SLLRLKEPAVLLRCRKSDVYLVESVLGSAAVEYAEKEKVHEPEIIVDHVHLPPGPSHHHQ 180
           SLLRLKEPAVLLRCR+ D + V  VL SA  EY EK  V  PE+IVD +HLPP P+ +  
Sbjct: 121 SLLRLKEPAVLLRCREEDKHHVHRVLHSAREEYGEKACVSHPEVIVDDIHLPPAPTSYDS 180

Query: 181 H 182
           H
Sbjct: 181 H 181

BLAST of Cucsat.G15448.T6 vs. NCBI nr
Match: XP_004148003.1 (V-type proton ATPase subunit E [Cucumis sativus] >KGN58149.1 hypothetical protein Csa_017428 [Cucumis sativus])

HSP 1 Score: 338 bits (866), Expect = 9.69e-116
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 0

Query: 1   MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK 60
           MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK
Sbjct: 1   MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK 60

Query: 61  QVEIRKKIEYSMQLNASRIKVLQAQDDVVNDMKEAASKELLSIGQNEHVYKNLLKDLIVQ 120
           QVEIRKKIEYSMQLNASRIKVLQAQDDVVNDMKEAASKELLSIGQNEHVYKNLLKDLIVQ
Sbjct: 61  QVEIRKKIEYSMQLNASRIKVLQAQDDVVNDMKEAASKELLSIGQNEHVYKNLLKDLIVQ 120

Query: 121 SLLRLKEPAVLLRCRKSDVYLVESVLGSAAVEYAEKEKVHEPEIIVDHVHLPPGPSHHHQ 180
           SLLRLKEPAVLLRCRKSDVYLVESVLGSAAVEYAEKEKVHEPEIIVDHVHLPPGPSHHHQ
Sbjct: 121 SLLRLKEPAVLLRCRKSDVYLVESVLGSAAVEYAEKEKVHEPEIIVDHVHLPPGPSHHHQ 180

Query: 181 HGPS 184
           HGPS
Sbjct: 181 HGPS 184

BLAST of Cucsat.G15448.T6 vs. NCBI nr
Match: XP_008450144.1 (PREDICTED: V-type proton ATPase subunit E [Cucumis melo])

HSP 1 Score: 327 bits (838), Expect = 1.78e-111
Identity = 178/184 (96.74%), Postives = 181/184 (98.37%), Query Frame = 0

Query: 1   MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK 60
           MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK
Sbjct: 1   MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK 60

Query: 61  QVEIRKKIEYSMQLNASRIKVLQAQDDVVNDMKEAASKELLSIGQNEHVYKNLLKDLIVQ 120
           QVEIRKKIEYSMQLNASRIKVLQAQDDVVN+MKEAASKELLS+ QNEHVYKNLLKDLIVQ
Sbjct: 61  QVEIRKKIEYSMQLNASRIKVLQAQDDVVNNMKEAASKELLSVSQNEHVYKNLLKDLIVQ 120

Query: 121 SLLRLKEPAVLLRCRKSDVYLVESVLGSAAVEYAEKEKVHEPEIIVDHVHLPPGPSHHHQ 180
           SLLRLKEPAVLLRCRKSDV+LVESVLGSAAVEYAEK KVHEPEIIVDHVHLPPGPSHHH 
Sbjct: 121 SLLRLKEPAVLLRCRKSDVHLVESVLGSAAVEYAEKAKVHEPEIIVDHVHLPPGPSHHHH 180

Query: 181 HGPS 184
           HGPS
Sbjct: 181 HGPS 184

BLAST of Cucsat.G15448.T6 vs. NCBI nr
Match: XP_038877402.1 (V-type proton ATPase subunit E-like [Benincasa hispida] >XP_038877403.1 V-type proton ATPase subunit E-like [Benincasa hispida])

HSP 1 Score: 321 bits (823), Expect = 3.44e-109
Identity = 175/183 (95.63%), Postives = 178/183 (97.27%), Query Frame = 0

Query: 1   MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK 60
           MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK
Sbjct: 1   MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK 60

Query: 61  QVEIRKKIEYSMQLNASRIKVLQAQDDVVNDMKEAASKELLSIGQNEHVYKNLLKDLIVQ 120
           QVEIRKKIEYSMQLNASRIKVLQAQDDVVNDMKEAASKELL++ QNEHVYKNLLKDLIVQ
Sbjct: 61  QVEIRKKIEYSMQLNASRIKVLQAQDDVVNDMKEAASKELLNVSQNEHVYKNLLKDLIVQ 120

Query: 121 SLLRLKEPAVLLRCRKSDVYLVESVLGSAAVEYAEKEKVHEPEIIVDHVHLPPGPSHHHQ 180
           SLLRLKEPAVLLRCRKSDV+LVESVLGSAAVEYAEK  VHEPEIIVDHVHLPPGPSHHH 
Sbjct: 121 SLLRLKEPAVLLRCRKSDVHLVESVLGSAAVEYAEKANVHEPEIIVDHVHLPPGPSHHHH 180

Query: 181 HGP 183
           H P
Sbjct: 181 HDP 183

BLAST of Cucsat.G15448.T6 vs. NCBI nr
Match: XP_022935239.1 (V-type proton ATPase subunit E-like [Cucurbita moschata])

HSP 1 Score: 317 bits (811), Expect = 2.31e-107
Identity = 173/182 (95.05%), Postives = 178/182 (97.80%), Query Frame = 0

Query: 1   MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK 60
           MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK
Sbjct: 1   MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK 60

Query: 61  QVEIRKKIEYSMQLNASRIKVLQAQDDVVNDMKEAASKELLSIGQNEHVYKNLLKDLIVQ 120
           QVEIRKKIEYSMQLNASRIKVLQAQDDVVN MKEAASKELL++ QNEHVYKNLLKDLIVQ
Sbjct: 61  QVEIRKKIEYSMQLNASRIKVLQAQDDVVNSMKEAASKELLNVSQNEHVYKNLLKDLIVQ 120

Query: 121 SLLRLKEPAVLLRCRKSDVYLVESVLGSAAVEYAEKEKVHEPEIIVDHVHLPPGPSHHHQ 180
           SLLRLKEPAVLLRCR+SDV+LVESVLGSAAVEYAEK KVHEPEIIVDHVHLPPGPSHH+ 
Sbjct: 121 SLLRLKEPAVLLRCRESDVHLVESVLGSAAVEYAEKVKVHEPEIIVDHVHLPPGPSHHNH 180

Query: 181 HG 182
           HG
Sbjct: 181 HG 182

BLAST of Cucsat.G15448.T6 vs. NCBI nr
Match: XP_023528922.1 (V-type proton ATPase subunit E-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 315 bits (807), Expect = 9.39e-107
Identity = 172/182 (94.51%), Postives = 177/182 (97.25%), Query Frame = 0

Query: 1   MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK 60
           MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK
Sbjct: 1   MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK 60

Query: 61  QVEIRKKIEYSMQLNASRIKVLQAQDDVVNDMKEAASKELLSIGQNEHVYKNLLKDLIVQ 120
           QVEIRKKIEYSMQLNASRIKVLQAQDDVVN MKEAASKELL++ QNEHVYKNLLKDLIVQ
Sbjct: 61  QVEIRKKIEYSMQLNASRIKVLQAQDDVVNSMKEAASKELLNVSQNEHVYKNLLKDLIVQ 120

Query: 121 SLLRLKEPAVLLRCRKSDVYLVESVLGSAAVEYAEKEKVHEPEIIVDHVHLPPGPSHHHQ 180
           SLLRLKEPAVLLRCR+SDV+LVE+VL SAAVEYAEK KVHEPEIIVDHVHLPPGPSHHH 
Sbjct: 121 SLLRLKEPAVLLRCRESDVHLVEAVLRSAAVEYAEKVKVHEPEIIVDHVHLPPGPSHHHH 180

Query: 181 HG 182
           HG
Sbjct: 181 HG 182

BLAST of Cucsat.G15448.T6 vs. ExPASy TrEMBL
Match: A0A0A0LB25 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G560240 PE=3 SV=1)

HSP 1 Score: 338 bits (866), Expect = 4.69e-116
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 0

Query: 1   MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK 60
           MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK
Sbjct: 1   MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK 60

Query: 61  QVEIRKKIEYSMQLNASRIKVLQAQDDVVNDMKEAASKELLSIGQNEHVYKNLLKDLIVQ 120
           QVEIRKKIEYSMQLNASRIKVLQAQDDVVNDMKEAASKELLSIGQNEHVYKNLLKDLIVQ
Sbjct: 61  QVEIRKKIEYSMQLNASRIKVLQAQDDVVNDMKEAASKELLSIGQNEHVYKNLLKDLIVQ 120

Query: 121 SLLRLKEPAVLLRCRKSDVYLVESVLGSAAVEYAEKEKVHEPEIIVDHVHLPPGPSHHHQ 180
           SLLRLKEPAVLLRCRKSDVYLVESVLGSAAVEYAEKEKVHEPEIIVDHVHLPPGPSHHHQ
Sbjct: 121 SLLRLKEPAVLLRCRKSDVYLVESVLGSAAVEYAEKEKVHEPEIIVDHVHLPPGPSHHHQ 180

Query: 181 HGPS 184
           HGPS
Sbjct: 181 HGPS 184

BLAST of Cucsat.G15448.T6 vs. ExPASy TrEMBL
Match: A0A1S3BNM0 (V-type proton ATPase subunit E OS=Cucumis melo OX=3656 GN=LOC103491820 PE=3 SV=1)

HSP 1 Score: 327 bits (838), Expect = 8.64e-112
Identity = 178/184 (96.74%), Postives = 181/184 (98.37%), Query Frame = 0

Query: 1   MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK 60
           MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK
Sbjct: 1   MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK 60

Query: 61  QVEIRKKIEYSMQLNASRIKVLQAQDDVVNDMKEAASKELLSIGQNEHVYKNLLKDLIVQ 120
           QVEIRKKIEYSMQLNASRIKVLQAQDDVVN+MKEAASKELLS+ QNEHVYKNLLKDLIVQ
Sbjct: 61  QVEIRKKIEYSMQLNASRIKVLQAQDDVVNNMKEAASKELLSVSQNEHVYKNLLKDLIVQ 120

Query: 121 SLLRLKEPAVLLRCRKSDVYLVESVLGSAAVEYAEKEKVHEPEIIVDHVHLPPGPSHHHQ 180
           SLLRLKEPAVLLRCRKSDV+LVESVLGSAAVEYAEK KVHEPEIIVDHVHLPPGPSHHH 
Sbjct: 121 SLLRLKEPAVLLRCRKSDVHLVESVLGSAAVEYAEKAKVHEPEIIVDHVHLPPGPSHHHH 180

Query: 181 HGPS 184
           HGPS
Sbjct: 181 HGPS 184

BLAST of Cucsat.G15448.T6 vs. ExPASy TrEMBL
Match: A0A6J1F4V1 (V-type proton ATPase subunit E-like OS=Cucurbita moschata OX=3662 GN=LOC111442179 PE=3 SV=1)

HSP 1 Score: 317 bits (811), Expect = 1.12e-107
Identity = 173/182 (95.05%), Postives = 178/182 (97.80%), Query Frame = 0

Query: 1   MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK 60
           MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK
Sbjct: 1   MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK 60

Query: 61  QVEIRKKIEYSMQLNASRIKVLQAQDDVVNDMKEAASKELLSIGQNEHVYKNLLKDLIVQ 120
           QVEIRKKIEYSMQLNASRIKVLQAQDDVVN MKEAASKELL++ QNEHVYKNLLKDLIVQ
Sbjct: 61  QVEIRKKIEYSMQLNASRIKVLQAQDDVVNSMKEAASKELLNVSQNEHVYKNLLKDLIVQ 120

Query: 121 SLLRLKEPAVLLRCRKSDVYLVESVLGSAAVEYAEKEKVHEPEIIVDHVHLPPGPSHHHQ 180
           SLLRLKEPAVLLRCR+SDV+LVESVLGSAAVEYAEK KVHEPEIIVDHVHLPPGPSHH+ 
Sbjct: 121 SLLRLKEPAVLLRCRESDVHLVESVLGSAAVEYAEKVKVHEPEIIVDHVHLPPGPSHHNH 180

Query: 181 HG 182
           HG
Sbjct: 181 HG 182

BLAST of Cucsat.G15448.T6 vs. ExPASy TrEMBL
Match: A0A6J1J2K4 (V-type proton ATPase subunit E-like OS=Cucurbita maxima OX=3661 GN=LOC111482103 PE=3 SV=1)

HSP 1 Score: 315 bits (807), Expect = 4.55e-107
Identity = 172/182 (94.51%), Postives = 177/182 (97.25%), Query Frame = 0

Query: 1   MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK 60
           MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKK+RQEYEKKEK
Sbjct: 1   MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKMRQEYEKKEK 60

Query: 61  QVEIRKKIEYSMQLNASRIKVLQAQDDVVNDMKEAASKELLSIGQNEHVYKNLLKDLIVQ 120
           QVEIRKKIEYSMQLNASRIKVLQAQDDVVN MKEAASKELL++ QNEHVYKNLLKDLIVQ
Sbjct: 61  QVEIRKKIEYSMQLNASRIKVLQAQDDVVNSMKEAASKELLNVSQNEHVYKNLLKDLIVQ 120

Query: 121 SLLRLKEPAVLLRCRKSDVYLVESVLGSAAVEYAEKEKVHEPEIIVDHVHLPPGPSHHHQ 180
           SLLRLKEPAVLLRCR+SDV+LVESVL SAAVEYAEK KVHEPEIIVDHVHLPPGPSHHH 
Sbjct: 121 SLLRLKEPAVLLRCRESDVHLVESVLRSAAVEYAEKVKVHEPEIIVDHVHLPPGPSHHHH 180

Query: 181 HG 182
           HG
Sbjct: 181 HG 182

BLAST of Cucsat.G15448.T6 vs. ExPASy TrEMBL
Match: A0A6J1EKB7 (V-type proton ATPase subunit E-like OS=Cucurbita moschata OX=3662 GN=LOC111435337 PE=3 SV=1)

HSP 1 Score: 310 bits (795), Expect = 3.06e-105
Identity = 169/183 (92.35%), Postives = 175/183 (95.63%), Query Frame = 0

Query: 1   MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK 60
           MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK
Sbjct: 1   MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK 60

Query: 61  QVEIRKKIEYSMQLNASRIKVLQAQDDVVNDMKEAASKELLSIGQNEHVYKNLLKDLIVQ 120
           QVEIRKKIEYSMQLNASRIKVLQAQDDVVN MKEAASKELL++  NEHVYKNLLK+LIVQ
Sbjct: 61  QVEIRKKIEYSMQLNASRIKVLQAQDDVVNSMKEAASKELLNVSGNEHVYKNLLKELIVQ 120

Query: 121 SLLRLKEPAVLLRCRKSDVYLVESVLGSAAVEYAEKEKVHEPEIIVDHVHLPPGPSHHHQ 180
           SLLRLKEPAVLLRCRKSD++LVESVL SAA EYA K +VHEPEIIVDHVHLPPGPSHHH 
Sbjct: 121 SLLRLKEPAVLLRCRKSDLHLVESVLSSAAYEYANKAEVHEPEIIVDHVHLPPGPSHHHH 180

Query: 181 HGP 183
           HGP
Sbjct: 181 HGP 183

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O239487.2e-7482.20V-type proton ATPase subunit E OS=Gossypium hirsutum OX=3635 GN=VATE PE=2 SV=1[more]
Q9SWE74.7e-7383.24V-type proton ATPase subunit E OS=Citrus limon OX=2708 GN=VATE PE=2 SV=1[more]
Q9MB463.0e-7282.70V-type proton ATPase subunit E OS=Citrus unshiu OX=55188 GN=VATE PE=2 SV=1[more]
Q413961.4e-6978.57V-type proton ATPase subunit E OS=Spinacia oleracea OX=3562 GN=VATE PE=2 SV=1[more]
Q402728.9e-6475.69V-type proton ATPase subunit E OS=Mesembryanthemum crystallinum OX=3544 GN=VATE ... [more]
Match NameE-valueIdentityDescription
XP_004148003.19.69e-116100.00V-type proton ATPase subunit E [Cucumis sativus] >KGN58149.1 hypothetical protei... [more]
XP_008450144.11.78e-11196.74PREDICTED: V-type proton ATPase subunit E [Cucumis melo][more]
XP_038877402.13.44e-10995.63V-type proton ATPase subunit E-like [Benincasa hispida] >XP_038877403.1 V-type p... [more]
XP_022935239.12.31e-10795.05V-type proton ATPase subunit E-like [Cucurbita moschata][more]
XP_023528922.19.39e-10794.51V-type proton ATPase subunit E-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A0A0LB254.69e-116100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G560240 PE=3 SV=1[more]
A0A1S3BNM08.64e-11296.74V-type proton ATPase subunit E OS=Cucumis melo OX=3656 GN=LOC103491820 PE=3 SV=1[more]
A0A6J1F4V11.12e-10795.05V-type proton ATPase subunit E-like OS=Cucurbita moschata OX=3662 GN=LOC11144217... [more]
A0A6J1J2K44.55e-10794.51V-type proton ATPase subunit E-like OS=Cucurbita maxima OX=3661 GN=LOC111482103 ... [more]
A0A6J1EKB73.06e-10592.35V-type proton ATPase subunit E-like OS=Cucurbita moschata OX=3662 GN=LOC11143533... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 38..61
NoneNo IPR availableGENE3D6.10.250.1620coord: 1..62
e-value: 1.1E-25
score: 91.4
NoneNo IPR availablePANTHERPTHR45715ATPASE H+-TRANSPORTING V1 SUBUNIT E1A-RELATEDcoord: 1..179
NoneNo IPR availablePANTHERPTHR45715:SF15BNAC03G72900D PROTEINcoord: 1..179
NoneNo IPR availableSUPERFAMILY160527V-type ATPase subunit E-likecoord: 87..159
IPR002842V-type ATPase subunit EPFAMPF01991vATP-synt_Ecoord: 16..168
e-value: 2.6E-50
score: 170.6
IPR038495V-type ATPase subunit E, C-terminal domain superfamilyGENE3D3.30.2320.30coord: 92..177
e-value: 3.9E-18
score: 67.5

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G15448Cucsat.G15448gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G15448.T6.E1Cucsat.G15448.T6.E1exon
Cucsat.G15448.T6.E2Cucsat.G15448.T6.E2exon
Cucsat.G15448.T6.E3Cucsat.G15448.T6.E3exon
Cucsat.G15448.T6.E4Cucsat.G15448.T6.E4exon
Cucsat.G15448.T6.E5Cucsat.G15448.T6.E5exon
Cucsat.G15448.T6.E6Cucsat.G15448.T6.E6exon
Cucsat.G15448.T6.E7Cucsat.G15448.T6.E7exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G15448.T6.C4Cucsat.G15448.T6.C4CDS
Cucsat.G15448.T6.C3Cucsat.G15448.T6.C3CDS
Cucsat.G15448.T6.C2Cucsat.G15448.T6.C2CDS
Cucsat.G15448.T6.C1Cucsat.G15448.T6.C1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G15448.T6Cucsat.G15448.T6-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902600 proton transmembrane transport
cellular_component GO:0033178 proton-transporting two-sector ATPase complex, catalytic domain
molecular_function GO:0046961 proton-transporting ATPase activity, rotational mechanism