Cucsat.G14631.T8 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G14631.T8
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Glutathione s-transferase
Location	ctg1869: 2705227 .. 2706571 (+)
RNA-Seq Expression	Cucsat.G14631.T8
Synteny	Cucsat.G14631.T8

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GGTGGCTTACATTTTTTGACATAATTTTTATGAAAGAGAAAGATTGCTCAGCAAATGATGTTGGCAATCAAAGTCATATTTTAACTCTTTGATCAAAATTAGATATTTTAGTTAAGATGTGAACAAAATCTCCAGTTCTTACGACTGTTTTGCCACGATTGCAATGGATGGTGACCATTCCTTGACTTGGGAGGAGCTGCCTTGTGGAATACGAATGGAGGACAGGAGACTCCATTTCGAGTTCCTCAGTATTCTTAAAACCACCTTCATCACACAATATTAACACCCTAGTTATGACTGCTAAACCGATCACAAAACCTTAGAGAAGATTAAGATATGACAGAGGAAAACAAAGTGGTGCTTTATGGATTGTGGGCGAGTCCTTTTGTAAAGAGGGTCGAACTCGCCCTTAAAATCAAAGCCATCCCTTTTGAATATGTAGAAGAAGATTTCCTGAACAAAAGCCCAGAGCTTCTCAAATTCAATCCTGTTTACAAGAAAGTTCCTGTACTCGTCCACAATGGAAGACCCATTTGCGAATCAGCTATAATTTTTGAGTACATAGAGGAAGTTTGGAACAACAACGGCCCTCCTCTTCTACCTCAAGATCCCTACAAAAGATCCCAGATTCGATTCTGGGCTGATTTCGTTCAGAATCAGGTAAATATATTTTATACAATAATTGATGCTCTTGACTTGTATTGTTCTTCCGGCCAATACACCTTTTTAGGCGAATAACTTTGTTGTGTGTTTTGTGTTGGTGCAGTTGTTTGATGGTCTACTTTTAGCAATGAAGACAGAAGGGGAAGCACAAGAGAAAGCCATAAAAGAAGTGAAAGAGAAGCTGAAAGTTATCGAAGAACAGGGCTTGAAGAGTTTGTTGGCAGAGGGAAGTCCATTTGTAAATGGAGATGAACTTGGATATTTAGACATAGGGATGTTGACAGTTCTTGGAAGGTACAAGATTTATGAAGAGTTTTTTGGAATGAAAATTATGGAAGAAGAGGAGATCCCAATTGTATTCTCGTGGTTGAATAGATTGATAGAGCACCCTATTGCAAAGGAGGGAGCTCCTCCCAAGGAAAAAGTTTTGGGGCTTCTTCACATCGTTAGGCGAAGATTTCTACCATCCCCTGTTACTGCTTAATGCTTCAACTCTATCTGTTCCTTCACATCAAAATCTACATTTTTTCTCTAATGTTTATGTGTATTTCACTCTATGGTCTTCAATAATAATATCTAATATGTTTTGATTCTTTTGTTGTTGTTTTTCTTTAATGAGATTTTGCAAACTGTCTATAAAACTCATTGACATCCTACTTGATTATAAGTTAAAGTACAGTAA

Coding sequence (CDS)

ATGACAGAGGAAAACAAAGTGGTGCTTTATGGATTGTGGGCGAGTCCTTTTGTAAAGAGGGTCGAACTCGCCCTTAAAATCAAAGCCATCCCTTTTGAATATGTAGAAGAAGATTTCCTGAACAAAAGCCCAGAGCTTCTCAAATTCAATCCTGTTTACAAGAAAGTTCCTGTACTCGTCCACAATGGAAGACCCATTTGCGAATCAGCTATAATTTTTGAGTACATAGAGGAAGTTTGGAACAACAACGGCCCTCCTCTTCTACCTCAAGATCCCTACAAAAGATCCCAGATTCGATTCTGGGCTGATTTCGTTCAGAATCAGTTGTTTGATGGTCTACTTTTAGCAATGAAGACAGAAGGGGAAGCACAAGAGAAAGCCATAAAAGAAGTGAAAGAGAAGCTGAAAGTTATCGAAGAACAGGGCTTGAAGAGTTTGTTGGCAGAGGGAAGTCCATTTGTAAATGGAGATGAACTTGGATATTTAGACATAGGGATGTTGACAGTTCTTGGAAGGTACAAGATTTATGAAGAGTTTTTTGGAATGAAAATTATGGAAGAAGAGGAGATCCCAATTGTATTCTCGTGGTTGAATAGATTGATAGAGCACCCTATTGCAAAGGAGGGAGCTCCTCCCAAGGAAAAAGTTTTGGGGCTTCTTCACATCGTTAGGCGAAGATTTCTACCATCCCCTGTTACTGCTTAA

Protein sequence

MTEENKVVLYGLWASPFVKRVELALKIKAIPFEYVEEDFLNKSPELLKFNPVYKKVPVLVHNGRPICESAIIFEYIEEVWNNNGPPLLPQDPYKRSQIRFWADFVQNQLFDGLLLAMKTEGEAQEKAIKEVKEKLKVIEEQGLKSLLAEGSPFVNGDELGYLDIGMLTVLGRYKIYEEFFGMKIMEEEEIPIVFSWLNRLIEHPIAKEGAPPKEKVLGLLHIVRRRFLPSPVTA

Homology

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BLAST of Cucsat.G14631.T8 vs. ExPASy Swiss-Prot
Match: Q9FUT0 (Glutathione S-transferase U9 OS=Arabidopsis thaliana OX=3702 GN=GSTU9 PE=2 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 1.0e-64
Identity = 116/231 (50.22%), Postives = 174/231 (75.32%), Query Frame = 0


Query: 4   ENKVVLYGLWASPFVKRVELALKIKAIPFEYVEEDFLNKSPELLKFNPVYKKVPVLVHNG 63
           ENKV+L+G +ASP+ KR+ELAL++K+IP+++V+ED  NKS  LL++NPV+KK+PVLVHNG
Sbjct: 6   ENKVILHGSFASPYSKRIELALRLKSIPYQFVQEDLQNKSQTLLRYNPVHKKIPVLVHNG 65

Query: 64  RPICESAIIFEYIEEVWNNNGPPLLPQDPYKRSQIRFWADFVQNQLFDGLLLAMKTEGEA 123
           +PI ES  I EYI+E W +NGP +LP+DPY+RS++RFWA+++Q  L+D ++  +K+EGE 
Sbjct: 66  KPISESLFIIEYIDETW-SNGPHILPEDPYRRSKVRFWANYIQLHLYDLVIKVVKSEGEE 125

Query: 124 QEKAIKEVKEKLKVIEEQGLKSLLA--EGSPFVNGDELGYLDIGMLTVLGRYKIYEEFFG 183
           Q+KA+ EVKEKL VIE++GLK + +  +G P V  + +  +DI M T+L  YK +EE  G
Sbjct: 126 QKKALTEVKEKLSVIEKEGLKEIFSDTDGEPTVTNETMSLVDIVMCTLLSPYKAHEEVLG 185

Query: 184 MKIMEEEEIPIVFSWLNRLIEHPIAKEGAPPKEKVLGLLHIVRRRFLPSPV 233
           +KI++ E +P V+ W+N + E  + K+ +PP E++L +L   R+  L SPV
Sbjct: 186 LKIIDPEIVPGVYGWINAINETSVVKDLSPPYEQILEILRAFRQMSL-SPV 234

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BLAST of Cucsat.G14631.T8 vs. ExPASy Swiss-Prot
Match: Q9CA57 (Glutathione S-transferase U10 OS=Arabidopsis thaliana OX=3702 GN=GSTU10 PE=2 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 3.5e-49
Identity = 91/232 (39.22%), Postives = 150/232 (64.66%), Query Frame = 0


Query: 3   EENKVVLYGLWASPFVKRVELALKIKAIPFEYVEEDFLNKSPELLKFNPVYKKVPVLVHN 62
           +++KV+L+G W S + KRVE+ALK+K + +EY+EED  NKS  L++ NPV+KK+PVLVH+
Sbjct: 4   KKSKVILHGTWISTYSKRVEIALKLKGVLYEYLEEDLQNKSESLIQLNPVHKKIPVLVHD 63

Query: 63  GRPICESAIIFEYIEEVWNNNGPPLLPQDPYKRSQIRFWADFVQNQLFDGLLLAMKTEGE 122
           G+P+ ES +I EYI+E W N+ P   P+DPY+R+Q+RFW  ++  Q+F+ +   M  EGE
Sbjct: 64  GKPVAESLVILEYIDETWTNS-PRFFPEDPYERAQVRFWVSYINQQVFEVMGQVMSQEGE 123

Query: 123 AQEKAIKEVKEKLKVIEEQGLKSLLAEGSPFVNGDELGYLDIGMLTVLGRYKIYEEFFGM 182
           AQ K+++E +++ KV++E GLK      +     D++G L+I ++  LG YK + E  G+
Sbjct: 124 AQAKSVEEARKRFKVLDE-GLKKHFPNKN-IRRNDDVGLLEITIIATLGGYKAHREAIGV 183

Query: 183 KIMEEEEIPIVFSWLNRLIEHPIAKEGAPPKEKVLGLLHIVRRRFLPSPVTA 235
            I+     P +++W+ RL +  + KE   P + ++  +   R++ L     A
Sbjct: 184 DIIGPVNTPTLYNWIERLQDLSVIKEVEVPHDTLVTFIQKYRQKCLQQAANA 232

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BLAST of Cucsat.G14631.T8 vs. ExPASy Swiss-Prot
Match: Q9FQA3 (Glutathione transferase GST 23 OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 6.2e-46
Identity = 94/225 (41.78%), Postives = 134/225 (59.56%), Query Frame = 0


Query: 4   ENKVVLYGLWASPFVKRVELALKIKAIPFEYVEEDFLNKSPELLKFNPVYKKVPVLVHNG 63
           E  V + G+WASP V RVE AL++K + +EYV+ED  NKS +LL+ NPV KKVPVLVH+G
Sbjct: 3   EKGVKVLGMWASPMVIRVEWALRLKGVEYEYVDEDLANKSADLLRHNPVTKKVPVLVHDG 62

Query: 64  RPICESAIIFEYIEEVWNNNGPPLLPQDPYKRSQIRFWADFVQNQLFDGLLLAMKTEGEA 123
           +P+ ES II EYI+EVW   G P++P DPY+R+Q RFWA F +++    L       GEA
Sbjct: 63  KPVAESTIIVEYIDEVW-KGGYPIMPGDPYERAQARFWARFAEDKCNAALYPIFTATGEA 122

Query: 124 QEKAIKEVKEKLKVIEEQGLKSLLAEGSPFVNGDELGYLDIGMLTVLGRYKIYEEFFGMK 183
           Q KA+ E ++ LK +E         EG  F  GD +GYLDI +        + EE  G  
Sbjct: 123 QRKAVHEAQQCLKTLE------TALEGKKFFGGDAVGYLDIVVGWFAHWLPVIEEVTGAS 182

Query: 184 IMEEEEIPIVFSWLNRLIEHPIAKEGAPPKEKVLGLLHIVRRRFL 229
           ++  EE+P++ +W  R +   + K   P ++++L      R + L
Sbjct: 183 VVTHEELPLMKAWFGRFLALDVVKAALPDRDRLLAANKARREQLL 220

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BLAST of Cucsat.G14631.T8 vs. ExPASy Swiss-Prot
Match: Q9ZRW8 (Glutathione S-transferase U19 OS=Arabidopsis thaliana OX=3702 GN=GSTU19 PE=1 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 2.4e-45
Identity = 89/225 (39.56%), Postives = 135/225 (60.00%), Query Frame = 0


Query: 5   NKVVLYGLWASPFVKRVELALKIKAIPFEYVEEDFLNKSPELLKFNPVYKKVPVLVHNGR 64
           N+V+L   W S F  R  +AL+ K + FEY EED  NKSP LL+ NP++KK+PVL+HNG+
Sbjct: 3   NEVILLDFWPSMFGMRTRIALREKGVEFEYREEDLRNKSPLLLQMNPIHKKIPVLIHNGK 62

Query: 65  PICESAIIFEYIEEVWNNNGPPLLPQDPYKRSQIRFWADFVQNQLFDGLLLAMKTEGEAQ 124
           P+ ES I  +YI+EVW++   P+LP DPY R+Q RFWADF+  +L+D       T+GE Q
Sbjct: 63  PVNESIIQVQYIDEVWSHKN-PILPSDPYLRAQARFWADFIDKKLYDAQRKVWATKGEEQ 122

Query: 125 EKAIKEVKEKLKVIEEQGLKSLLAEGSPFVNGDELGYLDIGMLTVLGRYKIYEEFFGMKI 184
           E   K+  E LK +E +          P+ +GD+ GY+DI ++     +  YE+F    I
Sbjct: 123 EAGKKDFIEILKTLESE------LGDKPYFSGDDFGYVDIALIGFYTWFPAYEKFANFSI 182

Query: 185 MEEEEIPIVFSWLNRLIEHPIAKEGAPPKEKVLGLLHIVRRRFLP 230
             E E+P + +W+ + ++     +  P  EKV   +  +R++F+P
Sbjct: 183 --ESEVPKLIAWVKKCLQRESVAKSLPDPEKVTEFVSELRKKFVP 218

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BLAST of Cucsat.G14631.T8 vs. ExPASy Swiss-Prot
Match: Q03666 (Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 2.0e-44
Identity = 92/222 (41.44%), Postives = 130/222 (58.56%), Query Frame = 0


Query: 6   KVVLYGLWASPFVKRVELALKIKAIPFEYVEEDFLNKSPELLKFNPVYKKVPVLVHNGRP 65
           +V+L   W S F  R+ +AL  K I +EY EED  NKSP LL+ NP++KK+PVL+HNG+P
Sbjct: 5   EVILLDFWPSMFGMRLRIALAEKEIKYEYKEEDLRNKSPLLLQMNPIHKKIPVLIHNGKP 64

Query: 66  ICESAIIFEYIEEVWNNNGPPLLPQDPYKRSQIRFWADFVQNQLFDGLLLAMKTEGEAQE 125
           ICES I  EYIEEVW +  P LLP DPY R+Q RFWAD++  +L+D       T+GE QE
Sbjct: 65  ICESIIAVEYIEEVWKDKAPNLLPSDPYDRAQARFWADYIDKKLYDFGRKLWTTKGEEQE 124

Query: 126 KAIKEVKEKLKVIEEQGLKSLLAEGSPFVNGDELGYLDIGMLTVLGRYKIYEEFFGMKIM 185
            A K+  E LKV+E       L +  P+  G+  G++DI ++     +  YE F      
Sbjct: 125 AAKKDFIECLKVLE-----GALGD-KPYFGGESFGFVDIALIGYYSWFYAYETFGNFS-- 184

Query: 186 EEEEIPIVFSWLNRLIEHPIAKEGAPPKEKVLGLLHIVRRRF 228
            E E P   +W  R ++     +  P + KVL  + ++R++F
Sbjct: 185 TEAECPKFVAWAKRCMQRESVAKSLPDQPKVLEFVKVLRQKF 218

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BLAST of Cucsat.G14631.T8 vs. NCBI nr
Match: XP_004151688.2 (glutathione S-transferase U9 [Cucumis sativus] >KGN63996.1 hypothetical protein Csa_013185 [Cucumis sativus])

HSP 1 Score: 475 bits (1223), Expect = 3.26e-169
Identity = 234/234 (100.00%), Postives = 234/234 (100.00%), Query Frame = 0


Query: 1   MTEENKVVLYGLWASPFVKRVELALKIKAIPFEYVEEDFLNKSPELLKFNPVYKKVPVLV 60
           MTEENKVVLYGLWASPFVKRVELALKIKAIPFEYVEEDFLNKSPELLKFNPVYKKVPVLV
Sbjct: 1   MTEENKVVLYGLWASPFVKRVELALKIKAIPFEYVEEDFLNKSPELLKFNPVYKKVPVLV 60

Query: 61  HNGRPICESAIIFEYIEEVWNNNGPPLLPQDPYKRSQIRFWADFVQNQLFDGLLLAMKTE 120
           HNGRPICESAIIFEYIEEVWNNNGPPLLPQDPYKRSQIRFWADFVQNQLFDGLLLAMKTE
Sbjct: 61  HNGRPICESAIIFEYIEEVWNNNGPPLLPQDPYKRSQIRFWADFVQNQLFDGLLLAMKTE 120

Query: 121 GEAQEKAIKEVKEKLKVIEEQGLKSLLAEGSPFVNGDELGYLDIGMLTVLGRYKIYEEFF 180
           GEAQEKAIKEVKEKLKVIEEQGLKSLLAEGSPFVNGDELGYLDIGMLTVLGRYKIYEEFF
Sbjct: 121 GEAQEKAIKEVKEKLKVIEEQGLKSLLAEGSPFVNGDELGYLDIGMLTVLGRYKIYEEFF 180

Query: 181 GMKIMEEEEIPIVFSWLNRLIEHPIAKEGAPPKEKVLGLLHIVRRRFLPSPVTA 234
           GMKIMEEEEIPIVFSWLNRLIEHPIAKEGAPPKEKVLGLLHIVRRRFLPSPVTA
Sbjct: 181 GMKIMEEEEIPIVFSWLNRLIEHPIAKEGAPPKEKVLGLLHIVRRRFLPSPVTA 234

[Close]

BLAST of Cucsat.G14631.T8 vs. NCBI nr
Match: TYJ98888.1 (glutathione S-transferase U9-like [Cucumis melo var. makuwa])

HSP 1 Score: 450 bits (1157), Expect = 3.76e-159
Identity = 220/232 (94.83%), Postives = 225/232 (96.98%), Query Frame = 0


Query: 1   MTEENKVVLYGLWASPFVKRVELALKIKAIPFEYVEEDFLNKSPELLKFNPVYKKVPVLV 60
           MTEENKVVLYGLWASPFVKRVELALKIK IPFEYVEEDFLNKSPELLKFNPVYKKVPVLV
Sbjct: 1   MTEENKVVLYGLWASPFVKRVELALKIKGIPFEYVEEDFLNKSPELLKFNPVYKKVPVLV 60

Query: 61  HNGRPICESAIIFEYIEEVWNNNGPPLLPQDPYKRSQIRFWADFVQNQLFDGLLLAMKTE 120
           HNGR ICESAIIFEYIEEVWN+NGP LLPQDPYKRSQ+RFWADFVQNQLFDGLLLAMKTE
Sbjct: 61  HNGRSICESAIIFEYIEEVWNDNGPSLLPQDPYKRSQVRFWADFVQNQLFDGLLLAMKTE 120

Query: 121 GEAQEKAIKEVKEKLKVIEEQGLKSLLAEGSPFVNGDELGYLDIGMLTVLGRYKIYEEFF 180
           GEAQEKAIKEVKEKLKV+EEQGLKSLL EGSPFVNGDELGYLDIGMLT+LGRYKIYEEFF
Sbjct: 121 GEAQEKAIKEVKEKLKVVEEQGLKSLLGEGSPFVNGDELGYLDIGMLTILGRYKIYEEFF 180

Query: 181 GMKIMEEEEIPIVFSWLNRLIEHPIAKEGAPPKEKVLGLLHIVRRRFLPSPV 232
           GMKIMEEEEIPIVFSWLNRLIEHPIAKEGA PKEKVLGLLHI R+RFL SPV
Sbjct: 181 GMKIMEEEEIPIVFSWLNRLIEHPIAKEGAHPKEKVLGLLHITRQRFLQSPV 232

[Close]

BLAST of Cucsat.G14631.T8 vs. NCBI nr
Match: KAA0036080.1 (glutathione S-transferase U9-like [Cucumis melo var. makuwa])

HSP 1 Score: 447 bits (1151), Expect = 1.01e-154
Identity = 218/229 (95.20%), Postives = 223/229 (97.38%), Query Frame = 0


Query: 4   ENKVVLYGLWASPFVKRVELALKIKAIPFEYVEEDFLNKSPELLKFNPVYKKVPVLVHNG 63
           ENKVVLYGLWASPFVKRVELALKIK IPFEYVEEDFLNKSPELLKFNPVYKKVPVLVHNG
Sbjct: 234 ENKVVLYGLWASPFVKRVELALKIKGIPFEYVEEDFLNKSPELLKFNPVYKKVPVLVHNG 293

Query: 64  RPICESAIIFEYIEEVWNNNGPPLLPQDPYKRSQIRFWADFVQNQLFDGLLLAMKTEGEA 123
           R ICESAIIFEYIEEVWN+NGP LLPQDPYKRSQ+RFWADFVQNQLFDGLLLAMKTEGEA
Sbjct: 294 RSICESAIIFEYIEEVWNDNGPSLLPQDPYKRSQVRFWADFVQNQLFDGLLLAMKTEGEA 353

Query: 124 QEKAIKEVKEKLKVIEEQGLKSLLAEGSPFVNGDELGYLDIGMLTVLGRYKIYEEFFGMK 183
           QEKAIKEVKEKLKV+EEQGLKSLL EGSPFVNGDELGYLDIGMLT+LGRYKIYEEFFGMK
Sbjct: 354 QEKAIKEVKEKLKVVEEQGLKSLLGEGSPFVNGDELGYLDIGMLTILGRYKIYEEFFGMK 413

Query: 184 IMEEEEIPIVFSWLNRLIEHPIAKEGAPPKEKVLGLLHIVRRRFLPSPV 232
           IMEEEEIPIVFSWLNRLIEHPIAKEGAPPKEKVLGLLHI R+RFL SPV
Sbjct: 414 IMEEEEIPIVFSWLNRLIEHPIAKEGAPPKEKVLGLLHITRQRFLQSPV 462

[Close]

BLAST of Cucsat.G14631.T8 vs. NCBI nr
Match: KAA0036079.1 (glutathione S-transferase U9-like [Cucumis melo var. makuwa])

HSP 1 Score: 438 bits (1126), Expect = 2.00e-154
Identity = 214/232 (92.24%), Postives = 219/232 (94.40%), Query Frame = 0


Query: 1   MTEENKVVLYGLWASPFVKRVELALKIKAIPFEYVEEDFLNKSPELLKFNPVYKKVPVLV 60
           MTEENKVVLYG WA PFVKRVEL LKIK IPF+YVEEDFLNKSPELLK NPVY+KVPVLV
Sbjct: 1   MTEENKVVLYGYWACPFVKRVELTLKIKGIPFDYVEEDFLNKSPELLKLNPVYRKVPVLV 60

Query: 61  HNGRPICESAIIFEYIEEVWNNNGPPLLPQDPYKRSQIRFWADFVQNQLFDGLLLAMKTE 120
           HNGR ICESAII EYIEEVWNNNGP LLPQDPYKRSQIRFWADF QNQLFDGLLLAMKTE
Sbjct: 61  HNGRSICESAIISEYIEEVWNNNGPSLLPQDPYKRSQIRFWADFAQNQLFDGLLLAMKTE 120

Query: 121 GEAQEKAIKEVKEKLKVIEEQGLKSLLAEGSPFVNGDELGYLDIGMLTVLGRYKIYEEFF 180
           GEAQEKAIKEVKEKLKV+EEQGLKSLL EGS FVNGDELGYLDIGMLT+LGRYKIYEEFF
Sbjct: 121 GEAQEKAIKEVKEKLKVVEEQGLKSLLGEGSSFVNGDELGYLDIGMLTILGRYKIYEEFF 180

Query: 181 GMKIMEEEEIPIVFSWLNRLIEHPIAKEGAPPKEKVLGLLHIVRRRFLPSPV 232
           GMKIMEEEEIPIVFSWLNRLIEHPIAKEGAPPKEKVLGLLHI R+RFL SPV
Sbjct: 181 GMKIMEEEEIPIVFSWLNRLIEHPIAKEGAPPKEKVLGLLHITRQRFLQSPV 232

[Close]

BLAST of Cucsat.G14631.T8 vs. NCBI nr
Match: TYJ98886.1 (glutathione S-transferase U9-like [Cucumis melo var. makuwa])

HSP 1 Score: 434 bits (1117), Expect = 4.72e-153
Identity = 213/232 (91.81%), Postives = 219/232 (94.40%), Query Frame = 0


Query: 1   MTEENKVVLYGLWASPFVKRVELALKIKAIPFEYVEEDFLNKSPELLKFNPVYKKVPVLV 60
           MTEENKVVLYG WA PFVKRVEL LKIK IPF+YVEEDFLNKSPELLK NPVY+KVPVLV
Sbjct: 1   MTEENKVVLYGYWACPFVKRVELTLKIKGIPFDYVEEDFLNKSPELLKLNPVYRKVPVLV 60

Query: 61  HNGRPICESAIIFEYIEEVWNNNGPPLLPQDPYKRSQIRFWADFVQNQLFDGLLLAMKTE 120
           HNGR ICESAII EYIEEVWNNNGP LLPQDPYKRSQI+FWADFVQNQLFDGLLLAMKTE
Sbjct: 61  HNGRSICESAIISEYIEEVWNNNGPSLLPQDPYKRSQIQFWADFVQNQLFDGLLLAMKTE 120

Query: 121 GEAQEKAIKEVKEKLKVIEEQGLKSLLAEGSPFVNGDELGYLDIGMLTVLGRYKIYEEFF 180
           GEAQEKAIKEVKEKLKV+EEQGLKSLL EGS FVNGDELGYLDIGMLT+LGRYKIYEEFF
Sbjct: 121 GEAQEKAIKEVKEKLKVVEEQGLKSLLGEGSSFVNGDELGYLDIGMLTILGRYKIYEEFF 180

Query: 181 GMKIMEEEEIPIVFSWLNRLIEHPIAKEGAPPKEKVLGLLHIVRRRFLPSPV 232
           GMKIMEEEEIPIVFSWLNRLIEHPIAKEGA PKEKVLGLLHI R+RFL SPV
Sbjct: 181 GMKIMEEEEIPIVFSWLNRLIEHPIAKEGAHPKEKVLGLLHITRQRFLQSPV 232

[Close]

BLAST of Cucsat.G14631.T8 vs. ExPASy TrEMBL
Match: A0A0A0LVI6 (Glutathione s-transferase OS=Cucumis sativus OX=3659 GN=Csa_1G033160 PE=3 SV=1)

HSP 1 Score: 475 bits (1223), Expect = 1.58e-169
Identity = 234/234 (100.00%), Postives = 234/234 (100.00%), Query Frame = 0


Query: 1   MTEENKVVLYGLWASPFVKRVELALKIKAIPFEYVEEDFLNKSPELLKFNPVYKKVPVLV 60
           MTEENKVVLYGLWASPFVKRVELALKIKAIPFEYVEEDFLNKSPELLKFNPVYKKVPVLV
Sbjct: 1   MTEENKVVLYGLWASPFVKRVELALKIKAIPFEYVEEDFLNKSPELLKFNPVYKKVPVLV 60

Query: 61  HNGRPICESAIIFEYIEEVWNNNGPPLLPQDPYKRSQIRFWADFVQNQLFDGLLLAMKTE 120
           HNGRPICESAIIFEYIEEVWNNNGPPLLPQDPYKRSQIRFWADFVQNQLFDGLLLAMKTE
Sbjct: 61  HNGRPICESAIIFEYIEEVWNNNGPPLLPQDPYKRSQIRFWADFVQNQLFDGLLLAMKTE 120

Query: 121 GEAQEKAIKEVKEKLKVIEEQGLKSLLAEGSPFVNGDELGYLDIGMLTVLGRYKIYEEFF 180
           GEAQEKAIKEVKEKLKVIEEQGLKSLLAEGSPFVNGDELGYLDIGMLTVLGRYKIYEEFF
Sbjct: 121 GEAQEKAIKEVKEKLKVIEEQGLKSLLAEGSPFVNGDELGYLDIGMLTVLGRYKIYEEFF 180

Query: 181 GMKIMEEEEIPIVFSWLNRLIEHPIAKEGAPPKEKVLGLLHIVRRRFLPSPVTA 234
           GMKIMEEEEIPIVFSWLNRLIEHPIAKEGAPPKEKVLGLLHIVRRRFLPSPVTA
Sbjct: 181 GMKIMEEEEIPIVFSWLNRLIEHPIAKEGAPPKEKVLGLLHIVRRRFLPSPVTA 234

[Close]

BLAST of Cucsat.G14631.T8 vs. ExPASy TrEMBL
Match: A0A5D3BL29 (Glutathione S-transferase U9-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00840 PE=3 SV=1)

HSP 1 Score: 450 bits (1157), Expect = 1.82e-159
Identity = 220/232 (94.83%), Postives = 225/232 (96.98%), Query Frame = 0


Query: 1   MTEENKVVLYGLWASPFVKRVELALKIKAIPFEYVEEDFLNKSPELLKFNPVYKKVPVLV 60
           MTEENKVVLYGLWASPFVKRVELALKIK IPFEYVEEDFLNKSPELLKFNPVYKKVPVLV
Sbjct: 1   MTEENKVVLYGLWASPFVKRVELALKIKGIPFEYVEEDFLNKSPELLKFNPVYKKVPVLV 60

Query: 61  HNGRPICESAIIFEYIEEVWNNNGPPLLPQDPYKRSQIRFWADFVQNQLFDGLLLAMKTE 120
           HNGR ICESAIIFEYIEEVWN+NGP LLPQDPYKRSQ+RFWADFVQNQLFDGLLLAMKTE
Sbjct: 61  HNGRSICESAIIFEYIEEVWNDNGPSLLPQDPYKRSQVRFWADFVQNQLFDGLLLAMKTE 120

Query: 121 GEAQEKAIKEVKEKLKVIEEQGLKSLLAEGSPFVNGDELGYLDIGMLTVLGRYKIYEEFF 180
           GEAQEKAIKEVKEKLKV+EEQGLKSLL EGSPFVNGDELGYLDIGMLT+LGRYKIYEEFF
Sbjct: 121 GEAQEKAIKEVKEKLKVVEEQGLKSLLGEGSPFVNGDELGYLDIGMLTILGRYKIYEEFF 180

Query: 181 GMKIMEEEEIPIVFSWLNRLIEHPIAKEGAPPKEKVLGLLHIVRRRFLPSPV 232
           GMKIMEEEEIPIVFSWLNRLIEHPIAKEGA PKEKVLGLLHI R+RFL SPV
Sbjct: 181 GMKIMEEEEIPIVFSWLNRLIEHPIAKEGAHPKEKVLGLLHITRQRFLQSPV 232

[Close]

BLAST of Cucsat.G14631.T8 vs. ExPASy TrEMBL
Match: A0A5A7SYD7 (Glutathione S-transferase U9-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold112G00740 PE=4 SV=1)

HSP 1 Score: 447 bits (1151), Expect = 4.91e-155
Identity = 218/229 (95.20%), Postives = 223/229 (97.38%), Query Frame = 0


Query: 4   ENKVVLYGLWASPFVKRVELALKIKAIPFEYVEEDFLNKSPELLKFNPVYKKVPVLVHNG 63
           ENKVVLYGLWASPFVKRVELALKIK IPFEYVEEDFLNKSPELLKFNPVYKKVPVLVHNG
Sbjct: 234 ENKVVLYGLWASPFVKRVELALKIKGIPFEYVEEDFLNKSPELLKFNPVYKKVPVLVHNG 293

Query: 64  RPICESAIIFEYIEEVWNNNGPPLLPQDPYKRSQIRFWADFVQNQLFDGLLLAMKTEGEA 123
           R ICESAIIFEYIEEVWN+NGP LLPQDPYKRSQ+RFWADFVQNQLFDGLLLAMKTEGEA
Sbjct: 294 RSICESAIIFEYIEEVWNDNGPSLLPQDPYKRSQVRFWADFVQNQLFDGLLLAMKTEGEA 353

Query: 124 QEKAIKEVKEKLKVIEEQGLKSLLAEGSPFVNGDELGYLDIGMLTVLGRYKIYEEFFGMK 183
           QEKAIKEVKEKLKV+EEQGLKSLL EGSPFVNGDELGYLDIGMLT+LGRYKIYEEFFGMK
Sbjct: 354 QEKAIKEVKEKLKVVEEQGLKSLLGEGSPFVNGDELGYLDIGMLTILGRYKIYEEFFGMK 413

Query: 184 IMEEEEIPIVFSWLNRLIEHPIAKEGAPPKEKVLGLLHIVRRRFLPSPV 232
           IMEEEEIPIVFSWLNRLIEHPIAKEGAPPKEKVLGLLHI R+RFL SPV
Sbjct: 414 IMEEEEIPIVFSWLNRLIEHPIAKEGAPPKEKVLGLLHITRQRFLQSPV 462

[Close]

BLAST of Cucsat.G14631.T8 vs. ExPASy TrEMBL
Match: A0A5A7T3V9 (Glutathione S-transferase U9-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold112G00730 PE=3 SV=1)

HSP 1 Score: 438 bits (1126), Expect = 9.70e-155
Identity = 214/232 (92.24%), Postives = 219/232 (94.40%), Query Frame = 0


Query: 1   MTEENKVVLYGLWASPFVKRVELALKIKAIPFEYVEEDFLNKSPELLKFNPVYKKVPVLV 60
           MTEENKVVLYG WA PFVKRVEL LKIK IPF+YVEEDFLNKSPELLK NPVY+KVPVLV
Sbjct: 1   MTEENKVVLYGYWACPFVKRVELTLKIKGIPFDYVEEDFLNKSPELLKLNPVYRKVPVLV 60

Query: 61  HNGRPICESAIIFEYIEEVWNNNGPPLLPQDPYKRSQIRFWADFVQNQLFDGLLLAMKTE 120
           HNGR ICESAII EYIEEVWNNNGP LLPQDPYKRSQIRFWADF QNQLFDGLLLAMKTE
Sbjct: 61  HNGRSICESAIISEYIEEVWNNNGPSLLPQDPYKRSQIRFWADFAQNQLFDGLLLAMKTE 120

Query: 121 GEAQEKAIKEVKEKLKVIEEQGLKSLLAEGSPFVNGDELGYLDIGMLTVLGRYKIYEEFF 180
           GEAQEKAIKEVKEKLKV+EEQGLKSLL EGS FVNGDELGYLDIGMLT+LGRYKIYEEFF
Sbjct: 121 GEAQEKAIKEVKEKLKVVEEQGLKSLLGEGSSFVNGDELGYLDIGMLTILGRYKIYEEFF 180

Query: 181 GMKIMEEEEIPIVFSWLNRLIEHPIAKEGAPPKEKVLGLLHIVRRRFLPSPV 232
           GMKIMEEEEIPIVFSWLNRLIEHPIAKEGAPPKEKVLGLLHI R+RFL SPV
Sbjct: 181 GMKIMEEEEIPIVFSWLNRLIEHPIAKEGAPPKEKVLGLLHITRQRFLQSPV 232

[Close]

BLAST of Cucsat.G14631.T8 vs. ExPASy TrEMBL
Match: A0A5D3BIH2 (Glutathione S-transferase U9-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00820 PE=3 SV=1)

HSP 1 Score: 434 bits (1117), Expect = 2.28e-153
Identity = 213/232 (91.81%), Postives = 219/232 (94.40%), Query Frame = 0


Query: 1   MTEENKVVLYGLWASPFVKRVELALKIKAIPFEYVEEDFLNKSPELLKFNPVYKKVPVLV 60
           MTEENKVVLYG WA PFVKRVEL LKIK IPF+YVEEDFLNKSPELLK NPVY+KVPVLV
Sbjct: 1   MTEENKVVLYGYWACPFVKRVELTLKIKGIPFDYVEEDFLNKSPELLKLNPVYRKVPVLV 60

Query: 61  HNGRPICESAIIFEYIEEVWNNNGPPLLPQDPYKRSQIRFWADFVQNQLFDGLLLAMKTE 120
           HNGR ICESAII EYIEEVWNNNGP LLPQDPYKRSQI+FWADFVQNQLFDGLLLAMKTE
Sbjct: 61  HNGRSICESAIISEYIEEVWNNNGPSLLPQDPYKRSQIQFWADFVQNQLFDGLLLAMKTE 120

Query: 121 GEAQEKAIKEVKEKLKVIEEQGLKSLLAEGSPFVNGDELGYLDIGMLTVLGRYKIYEEFF 180
           GEAQEKAIKEVKEKLKV+EEQGLKSLL EGS FVNGDELGYLDIGMLT+LGRYKIYEEFF
Sbjct: 121 GEAQEKAIKEVKEKLKVVEEQGLKSLLGEGSSFVNGDELGYLDIGMLTILGRYKIYEEFF 180

Query: 181 GMKIMEEEEIPIVFSWLNRLIEHPIAKEGAPPKEKVLGLLHIVRRRFLPSPV 232
           GMKIMEEEEIPIVFSWLNRLIEHPIAKEGA PKEKVLGLLHI R+RFL SPV
Sbjct: 181 GMKIMEEEEIPIVFSWLNRLIEHPIAKEGAHPKEKVLGLLHITRQRFLQSPV 232

The following BLAST results are available for this feature:

BLAST of Cucsat.G14631.T8 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FUT0	1.0e-64	50.22	Glutathione S-transferase U9 OS=Arabidopsis thaliana OX=3702 GN=GSTU9 PE=2 SV=1	[more]
Q9CA57	3.5e-49	39.22	Glutathione S-transferase U10 OS=Arabidopsis thaliana OX=3702 GN=GSTU10 PE=2 SV=...	[more]
Q9FQA3	6.2e-46	41.78	Glutathione transferase GST 23 OS=Zea mays OX=4577 PE=2 SV=1	[more]
Q9ZRW8	2.4e-45	39.56	Glutathione S-transferase U19 OS=Arabidopsis thaliana OX=3702 GN=GSTU19 PE=1 SV=...	[more]
Q03666	2.0e-44	41.44	Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]

BLAST of Cucsat.G14631.T8 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004151688.2	3.26e-169	100.00	glutathione S-transferase U9 [Cucumis sativus] >KGN63996.1 hypothetical protein ...	[more]
TYJ98888.1	3.76e-159	94.83	glutathione S-transferase U9-like [Cucumis melo var. makuwa]	[more]
KAA0036080.1	1.01e-154	95.20	glutathione S-transferase U9-like [Cucumis melo var. makuwa]	[more]
KAA0036079.1	2.00e-154	92.24	glutathione S-transferase U9-like [Cucumis melo var. makuwa]	[more]
TYJ98886.1	4.72e-153	91.81	glutathione S-transferase U9-like [Cucumis melo var. makuwa]	[more]

BLAST of Cucsat.G14631.T8 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LVI6	1.58e-169	100.00	Glutathione s-transferase OS=Cucumis sativus OX=3659 GN=Csa_1G033160 PE=3 SV=1	[more]
A0A5D3BL29	1.82e-159	94.83	Glutathione S-transferase U9-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A5A7SYD7	4.91e-155	95.20	Glutathione S-transferase U9-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2...	[more]
A0A5A7T3V9	9.70e-155	92.24	Glutathione S-transferase U9-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2...	[more]
A0A5D3BIH2	2.28e-153	91.81	Glutathione S-transferase U9-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 121..141
None	No IPR available	GENE3D	3.40.30.10	Glutaredoxin	coord: 1..87 e-value: 6.5E-38 score: 130.6
None	No IPR available	GENE3D	1.20.1050.10		coord: 88..229 e-value: 5.2E-40 score: 138.8
None	No IPR available	PANTHER	PTHR11260:SF640	GLUTATHIONE S-TRANSFERASE	coord: 1..229
None	No IPR available	PANTHER	PTHR11260	GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING	coord: 1..229
None	No IPR available	PROSITE	PS51354	GLUTAREDOXIN_2	coord: 1..95 score: 8.809174
None	No IPR available	CDD	cd03058	GST_N_Tau	coord: 7..80 e-value: 1.87782E-39 score: 128.935
IPR004045	Glutathione S-transferase, N-terminal	PFAM	PF02798	GST_N	coord: 7..77 e-value: 6.1E-17 score: 61.7
IPR004045	Glutathione S-transferase, N-terminal	PROSITE	PS50404	GST_NTER	coord: 5..84 score: 22.021294
IPR004046	Glutathione S-transferase, C-terminal	PFAM	PF00043	GST_C	coord: 116..204 e-value: 7.9E-6 score: 26.0
IPR045073	Glutathione S-transferase Omega/Tau-like	SFLD	SFLDG01152	Main.3:_Omega-_and_Tau-like_	coord: 3..227 e-value: 0.0 score: 250.5
IPR040079	Glutathione Transferase family	SFLD	SFLDS00019	Glutathione_Transferase_(cyto	coord: 3..227 e-value: 0.0 score: 250.5
IPR010987	Glutathione S-transferase, C-terminal-like	PROSITE	PS50405	GST_CTER	coord: 91..219 score: 15.480224
IPR045074	Glutathione S-transferases Tau, C-terminal alpha helical domain, plant	CDD	cd03185	GST_C_Tau	coord: 92..220 e-value: 5.33973E-43 score: 140.006
IPR036282	Glutathione S-transferase, C-terminal domain superfamily	SUPERFAMILY	47616	GST C-terminal domain-like	coord: 78..225
IPR036249	Thioredoxin-like superfamily	SUPERFAMILY	52833	Thioredoxin-like	coord: 5..109

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G14631	Cucsat.G14631	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G14631.T8.E1	Cucsat.G14631.T8.E1	exon
Cucsat.G14631.T8.E2	Cucsat.G14631.T8.E2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G14631.T8.C1	Cucsat.G14631.T8.C1	CDS
Cucsat.G14631.T8.C2	Cucsat.G14631.T8.C2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G14631.T8	Cucsat.G14631.T8-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006749	glutathione metabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004364	glutathione transferase activity
molecular_function	GO:0005515	protein binding
molecular_function	GO:0008270	zinc ion binding