Cucsat.G13494.T2 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G13494.T2
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Protein kinase domain-containing protein
Location	ctg1838: 7682911 .. 7686883 (+)
RNA-Seq Expression	Cucsat.G13494.T2
Synteny	Cucsat.G13494.T2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTCAATTGAATTCCGCGGGAATTTATTAAGTTTAGCTGTTAATGAGACTTTTCTACTCTCTCCCATAGACTTCTTACAAGTTTTGTCAAAATTTTGCCATGGCGGGTCCTCTCTTCACCGGCCCCATGAACATGAAATTTGGCATTGGAAGAAGCTTGGTAAGAGGTAAAGCAGTTCTACACCACAAACATATTACATTGACTCCACCGGTCAGCTCTGAAATTGATGTTTTTAAATCGTCGGTTGTCATAGTAGGGTTTTGTTTCTTCATCATTTAAATAATACATTCATTTTGACTCTTCTTGTTCATTTTACCTTTCGCTGTCTGGGATGGCCCAAATCATTGAATGAAATATTGAATTATTGGTGAGAAAGTCAAATTTTGAGTTTCTTAGGAATTGATTGTTAGAAAATTCATGTCCAGGCGGAGATTTTCTTTTTTGCCAGCATACCGAAACCTAACAAATCAACTACAAAATGTTGATTAGTAAAATAAGGTTTTAGAAAGCCTGTAGTTTGGGTCGAGACGCATATTAGAATTCCCTAGAGAAGATTTACCCCTATGTGTCGGGATTTTTTTTTTTTTTTTTTTTTTTAATTTAAAATTTGATATCCATGGCTGTTTGCATTCCTTAGACAACCCATCCTACAACATTTGAGTGAAACTCTAAAAAATTAATTTATAGGTAGGTGGCACCTGGATCAAACCGATGACTTCTTAGTAACTTATGTCCCCCATGATGGTTCTATGTGTTGGGAGTAGTTTGAAACTTCACTCTTAGGCTAGAACTCCCTACAAGAAAGCTTGTGTATAGCTAAGTTACGAATTTCTGTTAGACTTATATGCTCACGAAAGACCCTTTTGCAATTGAATGTTTCAAAACAAGTGGAATAAGGTCCTGGTAAAAATGTGTTCCATATGATATGATTTAAAAAAAGTAAAGAAGAGCTAACCTCATTCCGTAAGTAACTTAAAATTTTAGTTTAAAGAGGGAAGGGGAGCCTGACTCCTTTTCACCCATTTATTCAACAATAATGATTGGGACAAGCAAGGGCCATGGGAATGGTTCTTCGTCGAAGTTTCATCTCAAGTTCATTTATATCTAAGAAATTTTTTTGAATAGAAGGTGGTGGAAACGGGAAAAGGTCAAAGAGAACATGGGACTATGGGAGGAAAAAACTGCTGTGGATGAACCAGATGTAGCATTCTTCTGATTTTCCTATCGAGTAAGTGTAATTCTATATCTCATTGAATCTCTCTTGATTGAAAACTGCGATTAAAAAAATAAACATCTTTGGGCTACATGACAGGATTAGTTTGATTTCACCATATAATGACTTTGTTTTTCTTCACAAAATTTGATATGATTAGTTGGTACTTGGTATGATGCTCAGTTGATCAACCTTCAAACTGAGAAGTAAGCAAATCAGTGATGAGTTGTGAGTAGAATGGAATTTTGATATTTTAAGTAGTTGGGGAGAGAATTATTGAAAAGTTTTGAATGGCTAGAACTACTTGGGGAGGCTACATTGGTTATTATAGTTAGAGGTTGTTATAGTCTATGGATTATAATAGTTTGTGTTTAGGGTATAAATTATTTAAGTATAGGTAGGAAATATTAAACAATGTAACAATAGAGAAAGAGAGAATGAGTATTAAATAGTAAAAACATTGTAGAAAAGAGAGATTTGAAATAGTAATACTCTAGTTAATTGTATGTTATTACAACTTGAGCCCCAAACATGAAGTGGGCTATAATTGTCCACTCTATCAACTTCATGTTGGTGGCCTAAACACATCCTTGAAGATCAAAGTCCCATTATAGGTAGGTTGAAGGGGTGAATGTGTTAGAACTTTTGTTTGGAATATATTATAAAAAGTTAGCTAATTGATAAGAGGAATCAGGTTCTTGTAAGCATATTTTGGAGAGTAGTCCTGCAAAAGTAGAAGACTCTTAAGAGCTTGGAAGTTTTACATTTCTTTTCTTAAATGAATCAATAACATTTATCTATTGCTTTTCTCCTTTAATTTCTGGCTCCTATCAGGTAGGTATGTTGAAAGGGACTACAAACGTTAGCACAATTAATGGGAGAGATCTAATCATTTCAATTTTCAAAATCTAAAAAATTCATGTTCACTGTGCTCCAAAATTAATTTACATTTCTTTTTAAATAGTAATACAATATCTTATTATTATAATTCAACTTTCTGAATCCTGGTTCGTATCATCGTTGTGGTAGTTCTTTTTTAAATACTTCTGCCTTTTCAGCTCCTGGGCTATGGCAGAATTGAAGATAGTGATTATAGCAATATGTTGTAGGAAAATACTAGAAACTTCAATAATCTATTAACAGACAAATAGAAAAACAGATTGATGTAGGTCGAGAAAGTGAGAGATTTAAACATAGGTTGATGTTTTGTTGTCAAATGGCATCATGTTGTTAACTCTTTATCACACTATCAACTTGTTTCCCTCAGAATGTCATCAGGGTTTTCTCCAACAAGTTACAATGGCGAAAATAATGCTGGAGCAGGATTATGTGAGTACCTTATCACTCTGTATGAAAAAATATGAAACATTATTGACACAAGTTAAAATTCTCTTTATCTCACAAAAATATTGACTTGCAGATATACTTTTGAGCTTTGTGATTTTTGCTGTCATTGGTGCAATATCTTGCTGCTTTGCTAGGAAGGTGAGGCAGGGTATTGTAAGAGAGTTGCAAACTGTAGTTGCCTCAGCTACACCAAATTTGACACATTCACCAAATGTAGTTCAGATTTGGGAAATCGATCAACCAACAATGGAGAAGTTCATCCGAGAAATGGCGGAAGAAAGGCCTGTAAGGTTCACTCCTCAACAACTGTATTGTTTCACGAGCAATTATTCCACACCACTTGGGTCAGGAGGATTTGGTTCGGTCTACAAAGGTCAGTTTCCAAATGGGGTGAAGATTGCTGTGAAGGTTCTGAAGAGGAACGCAGATAGACAGGCAGAGGAACAATTTATGGCTGAGGTAGGAACAATTGGAAGAACTTATCATATAAACTTGGTTAGGCTTTATGGTTTTTGTTATGATCAATATATGGGTGCATTAGTATTTGAGTATATGGAAAATGGATCCTTGGATAAATATTTGTTTGGCAAAAATCAAGATATTGATTGGAGAAAGTTACATGATGTAGCCATTGGGACTGCTAAAGGTCTTGCTTACTTGCATGAGGAATGCCAACAGAGAATCATTCATTATGATATAAAGCCGGCTAACATCTTGTTGGATGCAAATTTCTCTCCCAAAGTTGGTGATTTTGGACTTGCCAAGCTTTGCAACAGGGATATAACCCACATGTCCCTCACTGGTTATCGAGGCACGCCGGGATATTCGGCTCCAGAATTCTTGTTCTTTAATTATCCGATAACGCACAAATGCGATGTATATAGTTTCGGAATGGTATTGTTTGAGATTGTTGGAAGAAAAAGAAATGCTGGAGTTACAGATTCTGGGAACCCTGATTGGTTGCCGCAACATGTTTGGGATAATTATGAAAAGGGTAAACTAGAAGAGCTCACTCTAATGTGTGGCATTGAAGAAGACAATAAAGAGAGAGCTAACAGAATGTGTGAGGTTGCTTTGTGGTGCGTTCAAGACTCTCCAGACAACCGGCCGCCTATGAGTACAGTGGTGAGGATGCTTGAAGGAGGAGTAGAAATAATGCCACCACCAAAGCCATTTCTTTACTTGCAATCAACTGCAAAAAGAAACGCACAACAAACTAGTAATAGTTCAGATTATTCAACTTCCAATGAAGAAAGTGGGTCAGTATGGTACAAGGACACTCCAATAATGAAGAAGTACGAAATACAGATAGCTAGTTCTTGATTTGAAAGAAAAAAGATTGTCTTTTGGCTTAAACATGGTTTTTCTTCTTCTTTAAAACTTATGGAAAATGTAGTCATTTGGTTTATTTTT

Coding sequence (CDS)

ATGTCATCAGGGTTTTCTCCAACAAGTTACAATGGCGAAAATAATGCTGGAGCAGGATTATATATACTTTTGAGCTTTGTGATTTTTGCTGTCATTGGTGCAATATCTTGCTGCTTTGCTAGGAAGGTGAGGCAGGGTATTGTAAGAGAGTTGCAAACTGTAGTTGCCTCAGCTACACCAAATTTGACACATTCACCAAATGTAGTTCAGATTTGGGAAATCGATCAACCAACAATGGAGAAGTTCATCCGAGAAATGGCGGAAGAAAGGCCTGTAAGGTTCACTCCTCAACAACTGTATTGTTTCACGAGCAATTATTCCACACCACTTGGGTCAGGAGGATTTGGTTCGGTCTACAAAGGTCAGTTTCCAAATGGGGTGAAGATTGCTGTGAAGGTTCTGAAGAGGAACGCAGATAGACAGGCAGAGGAACAATTTATGGCTGAGGTAGGAACAATTGGAAGAACTTATCATATAAACTTGGTTAGGCTTTATGGTTTTTGTTATGATCAATATATGGGTGCATTAGTATTTGAGTATATGGAAAATGGATCCTTGGATAAATATTTGTTTGGCAAAAATCAAGATATTGATTGGAGAAAGTTACATGATGTAGCCATTGGGACTGCTAAAGGTCTTGCTTACTTGCATGAGGAATGCCAACAGAGAATCATTCATTATGATATAAAGCCGGCTAACATCTTGTTGGATGCAAATTTCTCTCCCAAAGTTGGTGATTTTGGACTTGCCAAGCTTTGCAACAGGGATATAACCCACATGTCCCTCACTGGTTATCGAGGCACGCCGGGATATTCGGCTCCAGAATTCTTGTTCTTTAATTATCCGATAACGCACAAATGCGATGTATATAGTTTCGGAATGGTATTGTTTGAGATTGTTGGAAGAAAAAGAAATGCTGGAGTTACAGATTCTGGGAACCCTGATTGGTTGCCGCAACATGTTTGGGATAATTATGAAAAGGGTAAACTAGAAGAGCTCACTCTAATGTGTGGCATTGAAGAAGACAATAAAGAGAGAGCTAACAGAATGTGTGAGGTTGCTTTGTGGTGCGTTCAAGACTCTCCAGACAACCGGCCGCCTATGAGTACAGTGGTGAGGATGCTTGAAGGAGGAGTAGAAATAATGCCACCACCAAAGCCATTTCTTTACTTGCAATCAACTGCAAAAAGAAACGCACAACAAACTAGTAATAGTTCAGATTATTCAACTTCCAATGAAGAAAGTGGGTCAGTATGGTACAAGGACACTCCAATAATGAAGAAGTACGAAATACAGATAGCTAGTTCTTGA

Protein sequence

MSSGFSPTSYNGENNAGAGLYILLSFVIFAVIGAISCCFARKVRQGIVRELQTVVASATPNLTHSPNVVQIWEIDQPTMEKFIREMAEERPVRFTPQQLYCFTSNYSTPLGSGGFGSVYKGQFPNGVKIAVKVLKRNADRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALVFEYMENGSLDKYLFGKNQDIDWRKLHDVAIGTAKGLAYLHEECQQRIIHYDIKPANILLDANFSPKVGDFGLAKLCNRDITHMSLTGYRGTPGYSAPEFLFFNYPITHKCDVYSFGMVLFEIVGRKRNAGVTDSGNPDWLPQHVWDNYEKGKLEELTLMCGIEEDNKERANRMCEVALWCVQDSPDNRPPMSTVVRMLEGGVEIMPPPKPFLYLQSTAKRNAQQTSNSSDYSTSNEESGSVWYKDTPIMKKYEIQIASS

Homology

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BLAST of Cucsat.G13494.T2 vs. ExPASy Swiss-Prot
Match: P93604 (Rust resistance kinase Lr10 OS=Triticum aestivum OX=4565 GN=LRK10 PE=2 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 2.2e-73
Identity = 140/319 (43.89%), Postives = 204/319 (63.95%), Query Frame = 0


Query: 79  MEKFIREMAEERPVRFTPQQLYCFTSNYSTPLGSGGFGSVYKGQFPNGVKIAVKVLKRNA 138
           +E F++     +P R+T  ++      +   +G GGFGSVYKG+ PNGV +AVK+L+ + 
Sbjct: 314 VEMFLKTYGTSKPTRYTFSEVKKMARRFKEKVGQGGFGSVYKGELPNGVPVAVKMLENST 373

Query: 139 DRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALVFEYMENGSLDKYLFGKNQDID 198
                E F+ EV TIG  +H N+VRL GFC +    AL++E+M N SL+KY+F  + +I 
Sbjct: 374 GE--GESFINEVATIGLIHHANIVRLLGFCSEGMRRALIYEFMPNESLEKYIFSDDSNIF 433

Query: 199 W-----RKLHDVAIGTAKGLAYLHEECQQRIIHYDIKPANILLDANFSPKVGDFGLAKLC 258
                  KL D+A+G A+G+ YLH+ C QRI+H+DIKP NILLD NF+PK+ DFGLAKLC
Sbjct: 434 QNLLVPEKLLDIALGIARGMEYLHQGCNQRILHFDIKPHNILLDYNFNPKISDFGLAKLC 493

Query: 259 NRDITHMSLTGYRGTPGYSAPEFLFFNY-PITHKCDVYSFGMVLFEIVGRKRNAGVTDSG 318
            RD + ++LT  RGT GY APE    N+  +++K DVYSFGM++ E+V  +RN+      
Sbjct: 494 ARDQSIVTLTAARGTMGYIAPELYSRNFGGVSYKADVYSFGMLVLEMVSGRRNSDPRIGS 553

Query: 319 NPD-WLPQHVWDNYEKGKLEELTLMCGIEEDNKERANRMCEVALWCVQDSPDNRPPMSTV 378
             D +LP+ +++    G  EEL L     ++ K++  ++  VALWC+Q +P NRP M+ V
Sbjct: 554 QDDVYLPEWIYEKVING--EELALTLETTQEEKDKVRQLAMVALWCIQWNPRNRPSMTKV 613

Query: 379 VRMLEGGVE-IMPPPKPFL 390
           V ML G ++ +  PPKPF+
Sbjct: 614 VNMLTGRLQSLQMPPKPFV 628

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BLAST of Cucsat.G13494.T2 vs. ExPASy Swiss-Prot
Match: Q8RWZ5 (G-type lectin S-receptor-like serine/threonine-protein kinase SD2-5 OS=Arabidopsis thaliana OX=3702 GN=SD25 PE=1 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 3.3e-69
Identity = 135/300 (45.00%), Postives = 191/300 (63.67%), Query Frame = 0


Query: 91  PVRFTPQQLYCFTSNYSTPLGSGGFGSVYKGQFPNGVKIAVKVLKRNADRQAEEQFMAEV 150
           P+RF  + L   T+N+S  LG GGFGSVY+G  P+G ++AVK  K     Q +++F AEV
Sbjct: 480 PIRFAYKDLQSATNNFSVKLGQGGFGSVYEGTLPDGSRLAVK--KLEGIGQGKKEFRAEV 539

Query: 151 GTIGRTYHINLVRLYGFCYDQYMGALVFEYMENGSLDKYLFGKNQD---IDWRKLHDVAI 210
             IG  +H++LVRL GFC +     L +E++  GSL++++F K      +DW    ++A+
Sbjct: 540 SIIGSIHHLHLVRLRGFCAEGAHRLLAYEFLSKGSLERWIFRKKDGDVLLDWDTRFNIAL 599

Query: 211 GTAKGLAYLHEECQQRIIHYDIKPANILLDANFSPKVGDFGLAKLCNRDITHMSLTGYRG 270
           GTAKGLAYLHE+C  RI+H DIKP NILLD NF+ KV DFGLAKL  R+ +H+  T  RG
Sbjct: 600 GTAKGLAYLHEDCDARIVHCDIKPENILLDDNFNAKVSDFGLAKLMTREQSHV-FTTMRG 659

Query: 271 TPGYSAPEFLFFNYPITHKCDVYSFGMVLFEIVGRKRNAGVTDSGNPDWLPQHVWDNYEK 330
           T GY APE++  NY I+ K DVYS+GMVL E++G ++N   +++      P   +   E+
Sbjct: 660 TRGYLAPEWI-TNYAISEKSDVYSYGMVLLELIGGRKNYDPSETSEKCHFPSFAFKKMEE 719

Query: 331 GKLEELT--LMCGIEEDNKERANRMCEVALWCVQDSPDNRPPMSTVVRMLEGGVEIMPPP 386
           GKL ++    M  ++  + ER  R  + ALWC+Q+    RP MS VV+MLEG   ++ PP
Sbjct: 720 GKLMDIVDGKMKNVDVTD-ERVQRAMKTALWCIQEDMQTRPSMSKVVQMLEGVFPVVQPP 774

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BLAST of Cucsat.G13494.T2 vs. ExPASy Swiss-Prot
Match: Q9XID3 (G-type lectin S-receptor-like serine/threonine-protein kinase At1g34300 OS=Arabidopsis thaliana OX=3702 GN=At1g34300 PE=1 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 9.7e-69
Identity = 159/417 (38.13%), Postives = 225/417 (53.96%), Query Frame = 0


Query: 13  ENNAGAGLYILLSFVIFAVIGAIS-------CCFARKVRQGIVRELQTVVASATPNLTHS 72
           +NN+   L+I+   VI  ++G ++       CC  +  R G +    T++          
Sbjct: 415 DNNSKVHLWIVAVAVIAGLLGLVAVEIGLWWCCCRKNPRFGTLSSHYTLL---------- 474

Query: 73  PNVVQIWEIDQPTMEKFIREMAEERPVRFTPQQLYCFTSNYSTPLGSGGFGSVYKGQFPN 132
                              E A   PV+FT ++L   T ++   LG+GGFG+VY+G   N
Sbjct: 475 -------------------EYASGAPVQFTYKELQRCTKSFKEKLGAGGFGTVYRGVLTN 534

Query: 133 GVKIAVKVLKRNADRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALVFEYMENGS 192
              +AVK L+     Q E+QF  EV TI  T+H+NLVRL GFC       LV+E+M NGS
Sbjct: 535 RTVVAVKQLE--GIEQGEKQFRMEVATISSTHHLNLVRLIGFCSQGRHRLLVYEFMRNGS 594

Query: 193 LDKYLFGKN--QDIDWRKLHDVAIGTAKGLAYLHEECQQRIIHYDIKPANILLDANFSPK 252
           LD +LF  +  + + W    ++A+GTAKG+ YLHEEC+  I+H DIKP NIL+D NF+ K
Sbjct: 595 LDNFLFTTDSAKFLTWEYRFNIALGTAKGITYLHEECRDCIVHCDIKPENILVDDNFAAK 654

Query: 253 VGDFGLAKLCNRDITHMSLTGYRGTPGYSAPEFLFFNYPITHKCDVYSFGMVLFEIVGRK 312
           V DFGLAKL N      +++  RGT GY APE+L  N PIT K DVYS+GMVL E+V  K
Sbjct: 655 VSDFGLAKLLNPKDNRYNMSSVRGTRGYLAPEWL-ANLPITSKSDVYSYGMVLLELVSGK 714

Query: 313 RNAGVTDSGNPDWLPQHVWDNYEKGKLEELTLMCGIEED---NKERANRMCEVALWCVQD 372
           RN  V++  N        ++ +EKG  + + L   + ED   + E+  RM + + WC+Q+
Sbjct: 715 RNFDVSEKTNHKKFSIWAYEEFEKGNTKAI-LDTRLSEDQTVDMEQVMRMVKTSFWCIQE 774

Query: 373 SPDNRPPMSTVVRMLEGGVEIMPPPKPFLYLQSTAKRNAQQTSNSSDYSTSNEESGS 418
            P  RP M  VV+MLEG  EI  P  P    + +   N+  TS++S +  S     S
Sbjct: 775 QPLQRPTMGKVVQMLEGITEIKNPLCPKTISEVSFSGNSMSTSHASMFVASGPTRSS 798

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BLAST of Cucsat.G13494.T2 vs. ExPASy Swiss-Prot
Match: Q9FF29 (PR5-like receptor kinase OS=Arabidopsis thaliana OX=3702 GN=PR5K PE=1 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 4.8e-68
Identity = 137/337 (40.65%), Postives = 216/337 (64.09%), Query Frame = 0


Query: 93  RFTPQQLYCFTSNYSTPLGSGGFGSVYKGQF-PNGVKIAVKVLKRNADRQAEEQFMAEVG 152
           R++  ++   T++++  LG GGFG+VYKG+   +G  +AVK+LK        E+F+ EV 
Sbjct: 320 RYSYTRVKKMTNSFAHVLGKGGFGTVYKGKLADSGRDVAVKILK--VSEGNGEEFINEVA 379

Query: 153 TIGRTYHINLVRLYGFCYDQYMGALVFEYMENGSLDKYLFGK-NQDIDWRKLHDVAIGTA 212
           ++ RT H+N+V L GFCY++   A+++E+M NGSLDKY+    +  ++W +L+DVA+G +
Sbjct: 380 SMSRTSHVNIVSLLGFCYEKNKRAIIYEFMPNGSLDKYISANMSTKMEWERLYDVAVGIS 439

Query: 213 KGLAYLHEECQQRIIHYDIKPANILLDANFSPKVGDFGLAKLCNRDITHMSLTGYRGTPG 272
           +GL YLH  C  RI+H+DIKP NIL+D N  PK+ DFGLAKLC    + +S+   RGT G
Sbjct: 440 RGLEYLHNRCVTRIVHFDIKPQNILMDENLCPKISDFGLAKLCKNKESIISMLHMRGTFG 499

Query: 273 YSAPEFLFFNY-PITHKCDVYSFGMVLFEIVGRKRNAGVTDSGNPD---WLPQHVWDNYE 332
           Y APE    N+  ++HK DVYS+GMV+ E++G K    V  SG+ +   + P+ V+ ++E
Sbjct: 500 YIAPEMFSKNFGAVSHKSDVYSYGMVVLEMIGAKNIEKVEYSGSNNGSMYFPEWVYKDFE 559

Query: 333 KGKLEELTLMCG--IEEDNKERANRMCEVALWCVQDSPDNRPPMSTVVRMLEGGVE-IMP 392
           KG   E+T + G  I ++ ++ A ++  VALWC+Q +P +RPPM  V+ MLEG +E +  
Sbjct: 560 KG---EITRIFGDSITDEEEKIAKKLVLVALWCIQMNPSDRPPMIKVIEMLEGNLEALQV 619

Query: 393 PPKPFLYLQSTAKRNAQQTSN--SSDYSTSNEESGSV 419
           PP P L+       +  + S+  S+ ++ S+ E G++
Sbjct: 620 PPNPLLFSPEETVPDTLEDSDDTSTFFNPSHFERGTL 651

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BLAST of Cucsat.G13494.T2 vs. ExPASy Swiss-Prot
Match: Q9FLV4 (G-type lectin S-receptor-like serine/threonine-protein kinase At5g24080 OS=Arabidopsis thaliana OX=3702 GN=At5g24080 PE=2 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 1.4e-67
Identity = 145/311 (46.62%), Postives = 191/311 (61.41%), Query Frame = 0


Query: 89  ERPVRFTPQQLYCFTSNYSTPLGSGGFGSVYKGQFPNGVKIAVKVLKRNADRQAEEQFMA 148
           + PV FT + L   T+N+S  LGSGGFG+VYKG       +AVK L R A    E +F+ 
Sbjct: 515 DSPVSFTYRDLQNCTNNFSQLLGSGGFGTVYKGTVAGETLVAVKRLDR-ALSHGEREFIT 574

Query: 149 EVGTIGRTYHINLVRLYGFCYDQYMGALVFEYMENGSLDKYLFGKNQD---IDWRKLHDV 208
           EV TIG  +H+NLVRL G+C +     LV+EYM NGSLDK++F   Q    +DWR   ++
Sbjct: 575 EVNTIGSMHHMNLVRLCGYCSEDSHRLLVYEYMINGSLDKWIFSSEQTANLLDWRTRFEI 634

Query: 209 AIGTAKGLAYLHEECQQRIIHYDIKPANILLDANFSPKVGDFGLAKLCNRDITHMSLTGY 268
           A+ TA+G+AY HE+C+ RIIH DIKP NILLD NF PKV DFGLAK+  R+ +H+ +T  
Sbjct: 635 AVATAQGIAYFHEQCRNRIIHCDIKPENILLDDNFCPKVSDFGLAKMMGREHSHV-VTMI 694

Query: 269 RGTPGYSAPEFLFFNYPITHKCDVYSFGMVLFEIVGRKRNAGVTDSGNPDWLPQHVWDNY 328
           RGT GY APE++  N PIT K DVYS+GM+L EIVG +RN  ++      + P   +   
Sbjct: 695 RGTRGYLAPEWV-SNRPITVKADVYSYGMLLLEIVGGRRNLDMSYDAEDFFYPGWAYKEL 754

Query: 329 EKG---KLEELTLMCGIEEDNKERANRMCEVALWCVQDSPDNRPPMSTVVRMLEG-GVEI 388
             G   K  +  L  G+ E+  E   +  +VA WC+QD    RP M  VV++LEG   EI
Sbjct: 755 TNGTSLKAVDKRLQ-GVAEE--EEVVKALKVAFWCIQDEVSMRPSMGEVVKLLEGTSDEI 814

Query: 389 MPPPKPFLYLQ 393
             PP P   L+
Sbjct: 815 NLPPMPQTILE 819

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BLAST of Cucsat.G13494.T2 vs. NCBI nr
Match: XP_011652526.2 (rust resistance kinase Lr10 isoform X2 [Cucumis sativus] >XP_011652527.2 rust resistance kinase Lr10 isoform X2 [Cucumis sativus] >KAE8651374.1 hypothetical protein Csa_001444 [Cucumis sativus])

HSP 1 Score: 888 bits (2295), Expect = 0.0
Identity = 434/436 (99.54%), Postives = 434/436 (99.54%), Query Frame = 0


Query: 1   MSSGFSPTSYNGENNAGAGLYILLSFVIFAVIGAISCCFARKVRQGIVRELQTVVASATP 60
           MSSGFSPTS NGENNAGAGLYILLSFVIFAVIGAISCCFARKVRQGIVRELQTVVASATP
Sbjct: 1   MSSGFSPTSDNGENNAGAGLYILLSFVIFAVIGAISCCFARKVRQGIVRELQTVVASATP 60

Query: 61  NLTHSPNVVQIWEIDQPTMEKFIREMAEERPVRFTPQQLYCFTSNYSTPLGSGGFGSVYK 120
           NLTHSPNVVQIWEIDQPTMEKFIREMAEERPVR TPQQLYCFTSNYSTPLGSGGFGSVYK
Sbjct: 61  NLTHSPNVVQIWEIDQPTMEKFIREMAEERPVRLTPQQLYCFTSNYSTPLGSGGFGSVYK 120

Query: 121 GQFPNGVKIAVKVLKRNADRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALVFEY 180
           GQFPNGVKIAVKVLKRNADRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALVFEY
Sbjct: 121 GQFPNGVKIAVKVLKRNADRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALVFEY 180

Query: 181 MENGSLDKYLFGKNQDIDWRKLHDVAIGTAKGLAYLHEECQQRIIHYDIKPANILLDANF 240
           MENGSLDKYLFGKNQDIDWRKLHDVAIGTAKGLAYLHEECQQRIIHYDIKPANILLDANF
Sbjct: 181 MENGSLDKYLFGKNQDIDWRKLHDVAIGTAKGLAYLHEECQQRIIHYDIKPANILLDANF 240

Query: 241 SPKVGDFGLAKLCNRDITHMSLTGYRGTPGYSAPEFLFFNYPITHKCDVYSFGMVLFEIV 300
           SPKVGDFGLAKLCNRDITHMSLTGYRGTPGYSAPEFLFFNYPITHKCDVYSFGMVLFEIV
Sbjct: 241 SPKVGDFGLAKLCNRDITHMSLTGYRGTPGYSAPEFLFFNYPITHKCDVYSFGMVLFEIV 300

Query: 301 GRKRNAGVTDSGNPDWLPQHVWDNYEKGKLEELTLMCGIEEDNKERANRMCEVALWCVQD 360
           GRKRNAGVTDSGNPDWLPQHVWDNYEKGKLEELTLMCGIEEDNKERANRMCEVALWCVQD
Sbjct: 301 GRKRNAGVTDSGNPDWLPQHVWDNYEKGKLEELTLMCGIEEDNKERANRMCEVALWCVQD 360

Query: 361 SPDNRPPMSTVVRMLEGGVEIMPPPKPFLYLQSTAKRNAQQTSNSSDYSTSNEESGSVWY 420
           SPDNRPPMSTVVRMLEGGVEIMPPPKPFLYLQSTAKRNAQQTSNSSDYSTSNEESGSVWY
Sbjct: 361 SPDNRPPMSTVVRMLEGGVEIMPPPKPFLYLQSTAKRNAQQTSNSSDYSTSNEESGSVWY 420

Query: 421 KDTPIMKKYEIQIASS 436
           KDTPIMKKYEIQIASS
Sbjct: 421 KDTPIMKKYEIQIASS 436

[Close]

BLAST of Cucsat.G13494.T2 vs. NCBI nr
Match: XP_031738772.1 (rust resistance kinase Lr10 isoform X1 [Cucumis sativus] >XP_031738773.1 rust resistance kinase Lr10 isoform X1 [Cucumis sativus])

HSP 1 Score: 888 bits (2295), Expect = 0.0
Identity = 434/436 (99.54%), Postives = 434/436 (99.54%), Query Frame = 0


Query: 1   MSSGFSPTSYNGENNAGAGLYILLSFVIFAVIGAISCCFARKVRQGIVRELQTVVASATP 60
           MSSGFSPTS NGENNAGAGLYILLSFVIFAVIGAISCCFARKVRQGIVRELQTVVASATP
Sbjct: 23  MSSGFSPTSDNGENNAGAGLYILLSFVIFAVIGAISCCFARKVRQGIVRELQTVVASATP 82

Query: 61  NLTHSPNVVQIWEIDQPTMEKFIREMAEERPVRFTPQQLYCFTSNYSTPLGSGGFGSVYK 120
           NLTHSPNVVQIWEIDQPTMEKFIREMAEERPVR TPQQLYCFTSNYSTPLGSGGFGSVYK
Sbjct: 83  NLTHSPNVVQIWEIDQPTMEKFIREMAEERPVRLTPQQLYCFTSNYSTPLGSGGFGSVYK 142

Query: 121 GQFPNGVKIAVKVLKRNADRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALVFEY 180
           GQFPNGVKIAVKVLKRNADRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALVFEY
Sbjct: 143 GQFPNGVKIAVKVLKRNADRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALVFEY 202

Query: 181 MENGSLDKYLFGKNQDIDWRKLHDVAIGTAKGLAYLHEECQQRIIHYDIKPANILLDANF 240
           MENGSLDKYLFGKNQDIDWRKLHDVAIGTAKGLAYLHEECQQRIIHYDIKPANILLDANF
Sbjct: 203 MENGSLDKYLFGKNQDIDWRKLHDVAIGTAKGLAYLHEECQQRIIHYDIKPANILLDANF 262

Query: 241 SPKVGDFGLAKLCNRDITHMSLTGYRGTPGYSAPEFLFFNYPITHKCDVYSFGMVLFEIV 300
           SPKVGDFGLAKLCNRDITHMSLTGYRGTPGYSAPEFLFFNYPITHKCDVYSFGMVLFEIV
Sbjct: 263 SPKVGDFGLAKLCNRDITHMSLTGYRGTPGYSAPEFLFFNYPITHKCDVYSFGMVLFEIV 322

Query: 301 GRKRNAGVTDSGNPDWLPQHVWDNYEKGKLEELTLMCGIEEDNKERANRMCEVALWCVQD 360
           GRKRNAGVTDSGNPDWLPQHVWDNYEKGKLEELTLMCGIEEDNKERANRMCEVALWCVQD
Sbjct: 323 GRKRNAGVTDSGNPDWLPQHVWDNYEKGKLEELTLMCGIEEDNKERANRMCEVALWCVQD 382

Query: 361 SPDNRPPMSTVVRMLEGGVEIMPPPKPFLYLQSTAKRNAQQTSNSSDYSTSNEESGSVWY 420
           SPDNRPPMSTVVRMLEGGVEIMPPPKPFLYLQSTAKRNAQQTSNSSDYSTSNEESGSVWY
Sbjct: 383 SPDNRPPMSTVVRMLEGGVEIMPPPKPFLYLQSTAKRNAQQTSNSSDYSTSNEESGSVWY 442

Query: 421 KDTPIMKKYEIQIASS 436
           KDTPIMKKYEIQIASS
Sbjct: 443 KDTPIMKKYEIQIASS 458

[Close]

BLAST of Cucsat.G13494.T2 vs. NCBI nr
Match: XP_008466316.1 (PREDICTED: rust resistance kinase Lr10-like [Cucumis melo])

HSP 1 Score: 843 bits (2179), Expect = 4.22e-308
Identity = 413/437 (94.51%), Postives = 419/437 (95.88%), Query Frame = 0


Query: 1   MSSGFSPTSYNGENNAGAGLYILLSFVIFAVIGAISCCFARKVRQGIVRELQTVVASATP 60
           MSS FSP S NGENNAG  +YILLSFVIFAVIGAISCCFARKVRQGIVRELQ VVASA P
Sbjct: 1   MSSVFSPPSDNGENNAGTAIYILLSFVIFAVIGAISCCFARKVRQGIVRELQNVVASAPP 60

Query: 61  NLTHS-PNVVQIWEIDQPTMEKFIREMAEERPVRFTPQQLYCFTSNYSTPLGSGGFGSVY 120
             THS PNVVQIWEIDQPTMEKFIREMA+ERPVRFTPQQLYCFTSNYSTPLGSGGFGSVY
Sbjct: 61  TSTHSVPNVVQIWEIDQPTMEKFIREMAKERPVRFTPQQLYCFTSNYSTPLGSGGFGSVY 120

Query: 121 KGQFPNGVKIAVKVLKRNADRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALVFE 180
           KGQFPNGV IAVKVLKRN+DRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALVFE
Sbjct: 121 KGQFPNGVNIAVKVLKRNSDRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALVFE 180

Query: 181 YMENGSLDKYLFGKNQDIDWRKLHDVAIGTAKGLAYLHEECQQRIIHYDIKPANILLDAN 240
           YMENGSLDKYLFGKNQDIDWRKLHDVAIGTAKGLAYLHEECQQRIIHYDIKPANILLDAN
Sbjct: 181 YMENGSLDKYLFGKNQDIDWRKLHDVAIGTAKGLAYLHEECQQRIIHYDIKPANILLDAN 240

Query: 241 FSPKVGDFGLAKLCNRDITHMSLTGYRGTPGYSAPEFLFFNYPITHKCDVYSFGMVLFEI 300
           FSPKVGDFGLAKLCNRD THMSLTGYRGTPGYSAPEFLF+NYPITHKCDVYSFGMVLFEI
Sbjct: 241 FSPKVGDFGLAKLCNRDSTHMSLTGYRGTPGYSAPEFLFYNYPITHKCDVYSFGMVLFEI 300

Query: 301 VGRKRNAGVTDSGNPDWLPQHVWDNYEKGKLEELTLMCGIEEDNKERANRMCEVALWCVQ 360
           VGRKRNAGVTDSGNPDWLPQHVWDNY KGKLEE T MCGIEEDNKERANRMCEVALWCVQ
Sbjct: 301 VGRKRNAGVTDSGNPDWLPQHVWDNYGKGKLEEFTRMCGIEEDNKERANRMCEVALWCVQ 360

Query: 361 DSPDNRPPMSTVVRMLEGGVEIMPPPKPFLYLQSTAKRNAQQTSNSSDYSTSNEESGSVW 420
           DSPD+RPPMS VVRMLEGGVEIMPPPKPFLYL +TAKRN QQTSNSSDYSTSNEESGSVW
Sbjct: 361 DSPDDRPPMSAVVRMLEGGVEIMPPPKPFLYLDATAKRNVQQTSNSSDYSTSNEESGSVW 420

Query: 421 YKDTPIMKKYEIQIASS 436
           YKDTPIMKKYEIQIASS
Sbjct: 421 YKDTPIMKKYEIQIASS 437

[Close]

BLAST of Cucsat.G13494.T2 vs. NCBI nr
Match: KAA0038760.1 (rust resistance kinase Lr10-like [Cucumis melo var. makuwa] >TYK31375.1 rust resistance kinase Lr10-like [Cucumis melo var. makuwa])

HSP 1 Score: 841 bits (2173), Expect = 3.47e-307
Identity = 412/437 (94.28%), Postives = 418/437 (95.65%), Query Frame = 0


Query: 1   MSSGFSPTSYNGENNAGAGLYILLSFVIFAVIGAISCCFARKVRQGIVRELQTVVASATP 60
           MSS FSP S NGENNAG  +YILLSFVIFAVIGAISCCFARKVRQGIVRELQ VVASA P
Sbjct: 1   MSSVFSPPSDNGENNAGTAIYILLSFVIFAVIGAISCCFARKVRQGIVRELQNVVASAPP 60

Query: 61  NLTHS-PNVVQIWEIDQPTMEKFIREMAEERPVRFTPQQLYCFTSNYSTPLGSGGFGSVY 120
             THS PNVVQIWEIDQPTMEKFIREMA+ERPVRFTPQQLYCFTSNYSTPLGSGGFGSVY
Sbjct: 61  TSTHSVPNVVQIWEIDQPTMEKFIREMAKERPVRFTPQQLYCFTSNYSTPLGSGGFGSVY 120

Query: 121 KGQFPNGVKIAVKVLKRNADRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALVFE 180
           KGQFPNGV IAVKVLKRN+DRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALVFE
Sbjct: 121 KGQFPNGVNIAVKVLKRNSDRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALVFE 180

Query: 181 YMENGSLDKYLFGKNQDIDWRKLHDVAIGTAKGLAYLHEECQQRIIHYDIKPANILLDAN 240
           YMENGSLDKYLFGKNQDIDWRKLHDVAIGTAK LAYLHEECQQRIIHYDIKPANILLDAN
Sbjct: 181 YMENGSLDKYLFGKNQDIDWRKLHDVAIGTAKALAYLHEECQQRIIHYDIKPANILLDAN 240

Query: 241 FSPKVGDFGLAKLCNRDITHMSLTGYRGTPGYSAPEFLFFNYPITHKCDVYSFGMVLFEI 300
           FSPKVGDFGLAKLCNRD THMSLTGYRGTPGYSAPEFLF+NYPITHKCDVYSFGMVLFEI
Sbjct: 241 FSPKVGDFGLAKLCNRDSTHMSLTGYRGTPGYSAPEFLFYNYPITHKCDVYSFGMVLFEI 300

Query: 301 VGRKRNAGVTDSGNPDWLPQHVWDNYEKGKLEELTLMCGIEEDNKERANRMCEVALWCVQ 360
           VGRKRNAGVTDSGNPDWLPQHVWDNY KGKLEE T MCGIEEDNKERANRMCEVALWCVQ
Sbjct: 301 VGRKRNAGVTDSGNPDWLPQHVWDNYGKGKLEEFTRMCGIEEDNKERANRMCEVALWCVQ 360

Query: 361 DSPDNRPPMSTVVRMLEGGVEIMPPPKPFLYLQSTAKRNAQQTSNSSDYSTSNEESGSVW 420
           DSPD+RPPMS VVRMLEGGVEIMPPPKPFLYL +TAKRN QQTSNSSDYSTSNEESGSVW
Sbjct: 361 DSPDDRPPMSAVVRMLEGGVEIMPPPKPFLYLDATAKRNVQQTSNSSDYSTSNEESGSVW 420

Query: 421 YKDTPIMKKYEIQIASS 436
           YKDTPIMKKYEIQIASS
Sbjct: 421 YKDTPIMKKYEIQIASS 437

[Close]

BLAST of Cucsat.G13494.T2 vs. NCBI nr
Match: XP_022134951.1 (rust resistance kinase Lr10-like isoform X2 [Momordica charantia])

HSP 1 Score: 715 bits (1846), Expect = 2.28e-257
Identity = 350/429 (81.59%), Postives = 377/429 (87.88%), Query Frame = 0


Query: 1   MSSGFS-PTSYNGENNAGAGLYILLSFVIFAVIGAISCCFARKVRQGIVRELQTVVASAT 60
           MSSG S P + +G N     +Y++LS VIF+VIGAISCCFAR+VRQGIVRELQ+VV S  
Sbjct: 1   MSSGLSLPDNSSGSNTNSTVIYVVLSLVIFSVIGAISCCFARRVRQGIVRELQSVVTSPA 60

Query: 61  PNLTHSP--NVVQIWEIDQPTMEKFIREMAEERPVRFTPQQLYCFTSNYSTPLGSGGFGS 120
           PN+ HSP  N VQIWEID PTMEKF+RE+AEERPVRFT QQLY FTSNYS PLGSGGFG 
Sbjct: 61  PNVAHSPAQNTVQIWEIDAPTMEKFLREIAEERPVRFTAQQLYSFTSNYSAPLGSGGFGM 120

Query: 121 VYKGQFPNGVKIAVKVLKRNADRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALV 180
           VYKGQFPNG+KIAVK+LKR++DRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALV
Sbjct: 121 VYKGQFPNGMKIAVKILKRDSDRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALV 180

Query: 181 FEYMENGSLDKYLFGKNQDIDWRKLHDVAIGTAKGLAYLHEECQQRIIHYDIKPANILLD 240
           FEYMENGSLDKYLFGK Q+I+W KLHDVAIGTAKGLAYLHEECQQRIIHYDIKPANILLD
Sbjct: 181 FEYMENGSLDKYLFGKKQEIEWGKLHDVAIGTAKGLAYLHEECQQRIIHYDIKPANILLD 240

Query: 241 ANFSPKVGDFGLAKLCNRDITHMSLTGYRGTPGYSAPEFLFFNYPITHKCDVYSFGMVLF 300
           ANFSPKVGDFGLAKLCNRD THMSLTGYRGTPGYSAPEFL  N+PITHKCDVYSFGMVLF
Sbjct: 241 ANFSPKVGDFGLAKLCNRDSTHMSLTGYRGTPGYSAPEFLLSNFPITHKCDVYSFGMVLF 300

Query: 301 EIVGRKRNAGVTDSGNPDWLPQHVWDNYEKGKLEELTLMCGIEEDNKERANRMCEVALWC 360
           EI+GRKRNAGVT SGNPDW PQHVW+ Y K KLEE+T  CGIEE +KERA+RMC+VALWC
Sbjct: 301 EIIGRKRNAGVTPSGNPDWFPQHVWEEYRKLKLEEMTRSCGIEEKDKERASRMCKVALWC 360

Query: 361 VQDSPDNRPPMSTVVRMLEGGVEIMPPPKPFLYL-----QSTAKRNAQQTSNSSDYSTSN 420
           VQDSPD RPPMS VV+MLEGGVEIMPPPKPFLYL     +  +K N QQ   SSDYST N
Sbjct: 361 VQDSPDERPPMSAVVKMLEGGVEIMPPPKPFLYLNLLTTRGNSKLNLQQARTSSDYSTCN 420

[Close]

BLAST of Cucsat.G13494.T2 vs. ExPASy TrEMBL
Match: A0A0A0LHF1 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G881750 PE=3 SV=1)

HSP 1 Score: 886 bits (2289), Expect = 0.0
Identity = 433/436 (99.31%), Postives = 433/436 (99.31%), Query Frame = 0


Query: 1   MSSGFSPTSYNGENNAGAGLYILLSFVIFAVIGAISCCFARKVRQGIVRELQTVVASATP 60
           MSSGFSPTS NGENNAGAGLYILLSFVIFAVIGAISCCFARKVRQGIVRELQTVVASATP
Sbjct: 1   MSSGFSPTSDNGENNAGAGLYILLSFVIFAVIGAISCCFARKVRQGIVRELQTVVASATP 60

Query: 61  NLTHSPNVVQIWEIDQPTMEKFIREMAEERPVRFTPQQLYCFTSNYSTPLGSGGFGSVYK 120
           NLTHSPNVVQIWEIDQPTMEKFIREMAEERPVR TPQQLYCFTSNYSTPLGSGGFGSVYK
Sbjct: 61  NLTHSPNVVQIWEIDQPTMEKFIREMAEERPVRLTPQQLYCFTSNYSTPLGSGGFGSVYK 120

Query: 121 GQFPNGVKIAVKVLKRNADRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALVFEY 180
           GQFPNGVKIAVKVLKRNADRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALVFEY
Sbjct: 121 GQFPNGVKIAVKVLKRNADRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALVFEY 180

Query: 181 MENGSLDKYLFGKNQDIDWRKLHDVAIGTAKGLAYLHEECQQRIIHYDIKPANILLDANF 240
           MENGSLDKYLFGKNQDIDWRKLHDVAIGTAKGLAYLHEECQQRIIHYDIKPANILLDAN 
Sbjct: 181 MENGSLDKYLFGKNQDIDWRKLHDVAIGTAKGLAYLHEECQQRIIHYDIKPANILLDANL 240

Query: 241 SPKVGDFGLAKLCNRDITHMSLTGYRGTPGYSAPEFLFFNYPITHKCDVYSFGMVLFEIV 300
           SPKVGDFGLAKLCNRDITHMSLTGYRGTPGYSAPEFLFFNYPITHKCDVYSFGMVLFEIV
Sbjct: 241 SPKVGDFGLAKLCNRDITHMSLTGYRGTPGYSAPEFLFFNYPITHKCDVYSFGMVLFEIV 300

Query: 301 GRKRNAGVTDSGNPDWLPQHVWDNYEKGKLEELTLMCGIEEDNKERANRMCEVALWCVQD 360
           GRKRNAGVTDSGNPDWLPQHVWDNYEKGKLEELTLMCGIEEDNKERANRMCEVALWCVQD
Sbjct: 301 GRKRNAGVTDSGNPDWLPQHVWDNYEKGKLEELTLMCGIEEDNKERANRMCEVALWCVQD 360

Query: 361 SPDNRPPMSTVVRMLEGGVEIMPPPKPFLYLQSTAKRNAQQTSNSSDYSTSNEESGSVWY 420
           SPDNRPPMSTVVRMLEGGVEIMPPPKPFLYLQSTAKRNAQQTSNSSDYSTSNEESGSVWY
Sbjct: 361 SPDNRPPMSTVVRMLEGGVEIMPPPKPFLYLQSTAKRNAQQTSNSSDYSTSNEESGSVWY 420

Query: 421 KDTPIMKKYEIQIASS 436
           KDTPIMKKYEIQIASS
Sbjct: 421 KDTPIMKKYEIQIASS 436

[Close]

BLAST of Cucsat.G13494.T2 vs. ExPASy TrEMBL
Match: A0A1S3CR55 (rust resistance kinase Lr10-like OS=Cucumis melo OX=3656 GN=LOC103503762 PE=3 SV=1)

HSP 1 Score: 843 bits (2179), Expect = 2.05e-308
Identity = 413/437 (94.51%), Postives = 419/437 (95.88%), Query Frame = 0


Query: 1   MSSGFSPTSYNGENNAGAGLYILLSFVIFAVIGAISCCFARKVRQGIVRELQTVVASATP 60
           MSS FSP S NGENNAG  +YILLSFVIFAVIGAISCCFARKVRQGIVRELQ VVASA P
Sbjct: 1   MSSVFSPPSDNGENNAGTAIYILLSFVIFAVIGAISCCFARKVRQGIVRELQNVVASAPP 60

Query: 61  NLTHS-PNVVQIWEIDQPTMEKFIREMAEERPVRFTPQQLYCFTSNYSTPLGSGGFGSVY 120
             THS PNVVQIWEIDQPTMEKFIREMA+ERPVRFTPQQLYCFTSNYSTPLGSGGFGSVY
Sbjct: 61  TSTHSVPNVVQIWEIDQPTMEKFIREMAKERPVRFTPQQLYCFTSNYSTPLGSGGFGSVY 120

Query: 121 KGQFPNGVKIAVKVLKRNADRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALVFE 180
           KGQFPNGV IAVKVLKRN+DRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALVFE
Sbjct: 121 KGQFPNGVNIAVKVLKRNSDRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALVFE 180

Query: 181 YMENGSLDKYLFGKNQDIDWRKLHDVAIGTAKGLAYLHEECQQRIIHYDIKPANILLDAN 240
           YMENGSLDKYLFGKNQDIDWRKLHDVAIGTAKGLAYLHEECQQRIIHYDIKPANILLDAN
Sbjct: 181 YMENGSLDKYLFGKNQDIDWRKLHDVAIGTAKGLAYLHEECQQRIIHYDIKPANILLDAN 240

Query: 241 FSPKVGDFGLAKLCNRDITHMSLTGYRGTPGYSAPEFLFFNYPITHKCDVYSFGMVLFEI 300
           FSPKVGDFGLAKLCNRD THMSLTGYRGTPGYSAPEFLF+NYPITHKCDVYSFGMVLFEI
Sbjct: 241 FSPKVGDFGLAKLCNRDSTHMSLTGYRGTPGYSAPEFLFYNYPITHKCDVYSFGMVLFEI 300

Query: 301 VGRKRNAGVTDSGNPDWLPQHVWDNYEKGKLEELTLMCGIEEDNKERANRMCEVALWCVQ 360
           VGRKRNAGVTDSGNPDWLPQHVWDNY KGKLEE T MCGIEEDNKERANRMCEVALWCVQ
Sbjct: 301 VGRKRNAGVTDSGNPDWLPQHVWDNYGKGKLEEFTRMCGIEEDNKERANRMCEVALWCVQ 360

Query: 361 DSPDNRPPMSTVVRMLEGGVEIMPPPKPFLYLQSTAKRNAQQTSNSSDYSTSNEESGSVW 420
           DSPD+RPPMS VVRMLEGGVEIMPPPKPFLYL +TAKRN QQTSNSSDYSTSNEESGSVW
Sbjct: 361 DSPDDRPPMSAVVRMLEGGVEIMPPPKPFLYLDATAKRNVQQTSNSSDYSTSNEESGSVW 420

Query: 421 YKDTPIMKKYEIQIASS 436
           YKDTPIMKKYEIQIASS
Sbjct: 421 YKDTPIMKKYEIQIASS 437

[Close]

BLAST of Cucsat.G13494.T2 vs. ExPASy TrEMBL
Match: A0A5A7T688 (Rust resistance kinase Lr10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G006820 PE=3 SV=1)

HSP 1 Score: 841 bits (2173), Expect = 1.68e-307
Identity = 412/437 (94.28%), Postives = 418/437 (95.65%), Query Frame = 0


Query: 1   MSSGFSPTSYNGENNAGAGLYILLSFVIFAVIGAISCCFARKVRQGIVRELQTVVASATP 60
           MSS FSP S NGENNAG  +YILLSFVIFAVIGAISCCFARKVRQGIVRELQ VVASA P
Sbjct: 1   MSSVFSPPSDNGENNAGTAIYILLSFVIFAVIGAISCCFARKVRQGIVRELQNVVASAPP 60

Query: 61  NLTHS-PNVVQIWEIDQPTMEKFIREMAEERPVRFTPQQLYCFTSNYSTPLGSGGFGSVY 120
             THS PNVVQIWEIDQPTMEKFIREMA+ERPVRFTPQQLYCFTSNYSTPLGSGGFGSVY
Sbjct: 61  TSTHSVPNVVQIWEIDQPTMEKFIREMAKERPVRFTPQQLYCFTSNYSTPLGSGGFGSVY 120

Query: 121 KGQFPNGVKIAVKVLKRNADRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALVFE 180
           KGQFPNGV IAVKVLKRN+DRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALVFE
Sbjct: 121 KGQFPNGVNIAVKVLKRNSDRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALVFE 180

Query: 181 YMENGSLDKYLFGKNQDIDWRKLHDVAIGTAKGLAYLHEECQQRIIHYDIKPANILLDAN 240
           YMENGSLDKYLFGKNQDIDWRKLHDVAIGTAK LAYLHEECQQRIIHYDIKPANILLDAN
Sbjct: 181 YMENGSLDKYLFGKNQDIDWRKLHDVAIGTAKALAYLHEECQQRIIHYDIKPANILLDAN 240

Query: 241 FSPKVGDFGLAKLCNRDITHMSLTGYRGTPGYSAPEFLFFNYPITHKCDVYSFGMVLFEI 300
           FSPKVGDFGLAKLCNRD THMSLTGYRGTPGYSAPEFLF+NYPITHKCDVYSFGMVLFEI
Sbjct: 241 FSPKVGDFGLAKLCNRDSTHMSLTGYRGTPGYSAPEFLFYNYPITHKCDVYSFGMVLFEI 300

Query: 301 VGRKRNAGVTDSGNPDWLPQHVWDNYEKGKLEELTLMCGIEEDNKERANRMCEVALWCVQ 360
           VGRKRNAGVTDSGNPDWLPQHVWDNY KGKLEE T MCGIEEDNKERANRMCEVALWCVQ
Sbjct: 301 VGRKRNAGVTDSGNPDWLPQHVWDNYGKGKLEEFTRMCGIEEDNKERANRMCEVALWCVQ 360

Query: 361 DSPDNRPPMSTVVRMLEGGVEIMPPPKPFLYLQSTAKRNAQQTSNSSDYSTSNEESGSVW 420
           DSPD+RPPMS VVRMLEGGVEIMPPPKPFLYL +TAKRN QQTSNSSDYSTSNEESGSVW
Sbjct: 361 DSPDDRPPMSAVVRMLEGGVEIMPPPKPFLYLDATAKRNVQQTSNSSDYSTSNEESGSVW 420

Query: 421 YKDTPIMKKYEIQIASS 436
           YKDTPIMKKYEIQIASS
Sbjct: 421 YKDTPIMKKYEIQIASS 437

[Close]

BLAST of Cucsat.G13494.T2 vs. ExPASy TrEMBL
Match: A0A6J1BZ80 (rust resistance kinase Lr10-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007065 PE=3 SV=1)

HSP 1 Score: 715 bits (1846), Expect = 1.10e-257
Identity = 350/429 (81.59%), Postives = 377/429 (87.88%), Query Frame = 0


Query: 1   MSSGFS-PTSYNGENNAGAGLYILLSFVIFAVIGAISCCFARKVRQGIVRELQTVVASAT 60
           MSSG S P + +G N     +Y++LS VIF+VIGAISCCFAR+VRQGIVRELQ+VV S  
Sbjct: 1   MSSGLSLPDNSSGSNTNSTVIYVVLSLVIFSVIGAISCCFARRVRQGIVRELQSVVTSPA 60

Query: 61  PNLTHSP--NVVQIWEIDQPTMEKFIREMAEERPVRFTPQQLYCFTSNYSTPLGSGGFGS 120
           PN+ HSP  N VQIWEID PTMEKF+RE+AEERPVRFT QQLY FTSNYS PLGSGGFG 
Sbjct: 61  PNVAHSPAQNTVQIWEIDAPTMEKFLREIAEERPVRFTAQQLYSFTSNYSAPLGSGGFGM 120

Query: 121 VYKGQFPNGVKIAVKVLKRNADRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALV 180
           VYKGQFPNG+KIAVK+LKR++DRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALV
Sbjct: 121 VYKGQFPNGMKIAVKILKRDSDRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALV 180

Query: 181 FEYMENGSLDKYLFGKNQDIDWRKLHDVAIGTAKGLAYLHEECQQRIIHYDIKPANILLD 240
           FEYMENGSLDKYLFGK Q+I+W KLHDVAIGTAKGLAYLHEECQQRIIHYDIKPANILLD
Sbjct: 181 FEYMENGSLDKYLFGKKQEIEWGKLHDVAIGTAKGLAYLHEECQQRIIHYDIKPANILLD 240

Query: 241 ANFSPKVGDFGLAKLCNRDITHMSLTGYRGTPGYSAPEFLFFNYPITHKCDVYSFGMVLF 300
           ANFSPKVGDFGLAKLCNRD THMSLTGYRGTPGYSAPEFL  N+PITHKCDVYSFGMVLF
Sbjct: 241 ANFSPKVGDFGLAKLCNRDSTHMSLTGYRGTPGYSAPEFLLSNFPITHKCDVYSFGMVLF 300

Query: 301 EIVGRKRNAGVTDSGNPDWLPQHVWDNYEKGKLEELTLMCGIEEDNKERANRMCEVALWC 360
           EI+GRKRNAGVT SGNPDW PQHVW+ Y K KLEE+T  CGIEE +KERA+RMC+VALWC
Sbjct: 301 EIIGRKRNAGVTPSGNPDWFPQHVWEEYRKLKLEEMTRSCGIEEKDKERASRMCKVALWC 360

Query: 361 VQDSPDNRPPMSTVVRMLEGGVEIMPPPKPFLYL-----QSTAKRNAQQTSNSSDYSTSN 420
           VQDSPD RPPMS VV+MLEGGVEIMPPPKPFLYL     +  +K N QQ   SSDYST N
Sbjct: 361 VQDSPDERPPMSAVVKMLEGGVEIMPPPKPFLYLNLLTTRGNSKLNLQQARTSSDYSTCN 420

[Close]

BLAST of Cucsat.G13494.T2 vs. ExPASy TrEMBL
Match: A0A6J1C045 (rust resistance kinase Lr10-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007065 PE=4 SV=1)

HSP 1 Score: 715 bits (1846), Expect = 1.89e-256
Identity = 350/429 (81.59%), Postives = 377/429 (87.88%), Query Frame = 0


Query: 1   MSSGFS-PTSYNGENNAGAGLYILLSFVIFAVIGAISCCFARKVRQGIVRELQTVVASAT 60
           MSSG S P + +G N     +Y++LS VIF+VIGAISCCFAR+VRQGIVRELQ+VV S  
Sbjct: 78  MSSGLSLPDNSSGSNTNSTVIYVVLSLVIFSVIGAISCCFARRVRQGIVRELQSVVTSPA 137

Query: 61  PNLTHSP--NVVQIWEIDQPTMEKFIREMAEERPVRFTPQQLYCFTSNYSTPLGSGGFGS 120
           PN+ HSP  N VQIWEID PTMEKF+RE+AEERPVRFT QQLY FTSNYS PLGSGGFG 
Sbjct: 138 PNVAHSPAQNTVQIWEIDAPTMEKFLREIAEERPVRFTAQQLYSFTSNYSAPLGSGGFGM 197

Query: 121 VYKGQFPNGVKIAVKVLKRNADRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALV 180
           VYKGQFPNG+KIAVK+LKR++DRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALV
Sbjct: 198 VYKGQFPNGMKIAVKILKRDSDRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALV 257

Query: 181 FEYMENGSLDKYLFGKNQDIDWRKLHDVAIGTAKGLAYLHEECQQRIIHYDIKPANILLD 240
           FEYMENGSLDKYLFGK Q+I+W KLHDVAIGTAKGLAYLHEECQQRIIHYDIKPANILLD
Sbjct: 258 FEYMENGSLDKYLFGKKQEIEWGKLHDVAIGTAKGLAYLHEECQQRIIHYDIKPANILLD 317

Query: 241 ANFSPKVGDFGLAKLCNRDITHMSLTGYRGTPGYSAPEFLFFNYPITHKCDVYSFGMVLF 300
           ANFSPKVGDFGLAKLCNRD THMSLTGYRGTPGYSAPEFL  N+PITHKCDVYSFGMVLF
Sbjct: 318 ANFSPKVGDFGLAKLCNRDSTHMSLTGYRGTPGYSAPEFLLSNFPITHKCDVYSFGMVLF 377

Query: 301 EIVGRKRNAGVTDSGNPDWLPQHVWDNYEKGKLEELTLMCGIEEDNKERANRMCEVALWC 360
           EI+GRKRNAGVT SGNPDW PQHVW+ Y K KLEE+T  CGIEE +KERA+RMC+VALWC
Sbjct: 378 EIIGRKRNAGVTPSGNPDWFPQHVWEEYRKLKLEEMTRSCGIEEKDKERASRMCKVALWC 437

Query: 361 VQDSPDNRPPMSTVVRMLEGGVEIMPPPKPFLYL-----QSTAKRNAQQTSNSSDYSTSN 420
           VQDSPD RPPMS VV+MLEGGVEIMPPPKPFLYL     +  +K N QQ   SSDYST N
Sbjct: 438 VQDSPDERPPMSAVVKMLEGGVEIMPPPKPFLYLNLLTTRGNSKLNLQQARTSSDYSTCN 497

The following BLAST results are available for this feature:

BLAST of Cucsat.G13494.T2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P93604	2.2e-73	43.89	Rust resistance kinase Lr10 OS=Triticum aestivum OX=4565 GN=LRK10 PE=2 SV=1	[more]
Q8RWZ5	3.3e-69	45.00	G-type lectin S-receptor-like serine/threonine-protein kinase SD2-5 OS=Arabidops...	[more]
Q9XID3	9.7e-69	38.13	G-type lectin S-receptor-like serine/threonine-protein kinase At1g34300 OS=Arabi...	[more]
Q9FF29	4.8e-68	40.65	PR5-like receptor kinase OS=Arabidopsis thaliana OX=3702 GN=PR5K PE=1 SV=1	[more]
Q9FLV4	1.4e-67	46.62	G-type lectin S-receptor-like serine/threonine-protein kinase At5g24080 OS=Arabi...	[more]

BLAST of Cucsat.G13494.T2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011652526.2	0.0	99.54	rust resistance kinase Lr10 isoform X2 [Cucumis sativus] >XP_011652527.2 rust re...	[more]
XP_031738772.1	0.0	99.54	rust resistance kinase Lr10 isoform X1 [Cucumis sativus] >XP_031738773.1 rust re...	[more]
XP_008466316.1	4.22e-308	94.51	PREDICTED: rust resistance kinase Lr10-like [Cucumis melo]	[more]
KAA0038760.1	3.47e-307	94.28	rust resistance kinase Lr10-like [Cucumis melo var. makuwa] >TYK31375.1 rust res...	[more]
XP_022134951.1	2.28e-257	81.59	rust resistance kinase Lr10-like isoform X2 [Momordica charantia]	[more]

BLAST of Cucsat.G13494.T2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LHF1	0.0	99.31	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G881...	[more]
A0A1S3CR55	2.05e-308	94.51	rust resistance kinase Lr10-like OS=Cucumis melo OX=3656 GN=LOC103503762 PE=3 SV...	[more]
A0A5A7T688	1.68e-307	94.28	Rust resistance kinase Lr10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A6J1BZ80	1.10e-257	81.59	rust resistance kinase Lr10-like isoform X2 OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1C045	1.89e-256	81.59	rust resistance kinase Lr10-like isoform X1 OS=Momordica charantia OX=3673 GN=LO...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 104..375 e-value: 7.2E-28 score: 108.6
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 104..389 score: 37.940617
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 181..381 e-value: 9.9E-56 score: 190.3
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 76..180 e-value: 5.3E-24 score: 86.3
None	No IPR available	PIRSR	PIRSR000605-51	PIRSR000605-51	coord: 110..299 e-value: 2.2E-8 score: 30.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 395..416
None	No IPR available	PANTHER	PTHR27009:SF80	RECEPTOR SERINE/THREONINE KINASE	coord: 52..432
None	No IPR available	PANTHER	PTHR27009	RUST RESISTANCE KINASE LR10-RELATED	coord: 52..432
IPR001245	Serine-threonine/tyrosine-protein kinase, catalytic domain	PFAM	PF07714	PK_Tyr_Ser-Thr	coord: 108..375 e-value: 6.6E-46 score: 156.7
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 110..132
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 224..236
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 82..375

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G13494	Cucsat.G13494	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G13494.T2.E1	Cucsat.G13494.T2.E1	exon
Cucsat.G13494.T2.E2	Cucsat.G13494.T2.E2	exon
Cucsat.G13494.T2.E3	Cucsat.G13494.T2.E3	exon
Cucsat.G13494.T2.E4	Cucsat.G13494.T2.E4	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G13494.T2.C1	Cucsat.G13494.T2.C1	CDS
Cucsat.G13494.T2.C2	Cucsat.G13494.T2.C2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G13494.T2	Cucsat.G13494.T2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity
molecular_function	GO:0004674	protein serine/threonine kinase activity