Cucsat.G12823.T5 (mRNA) Cucumber (B10) v3

BLAST of Cucsat.G12823.T5 vs. ExPASy Swiss-Prot
Match: Q8LGN0 (Glutamate receptor 2.7 OS=Arabidopsis thaliana OX=3702 GN=GLR2.7 PE=2 SV=3)

HSP 1 Score: 706.4 bits (1822), Expect = 3.4e-202
Identity = 379/759 (49.93%), Postives = 514/759 (67.72%), Query Frame = 0

Query: 8   STFIIF--LSLCFGLRAFSVMADDEAVTVKVGLVLDSDSSIGKMGVSYMEMALSDFYESH 67
           +TF+ +  L +C  +     +  ++   +KVG+VLD  +S  K+ ++ + ++LSDFY+ H
Sbjct: 11  NTFMYYFVLFVCGFVLMEGCLGQNQTTEIKVGVVLDLHTSFSKLCLTSINISLSDFYKYH 70

Query: 68  RNYKTRLALFAKNSMEDVIEATAAAIELINKEEVEAIVGPQNSMQAAFMADLGKKSQVPI 127
            +Y TRLA+  ++SMEDV++A++AA++LI  E+V AI+GP+ SMQA FM  L  KSQVP 
Sbjct: 71  SDYTTRLAIHIRDSMEDVVQASSAALDLIKNEQVSAIIGPRTSMQAEFMIRLADKSQVPT 130

Query: 128 ISFSVTTPSLNPHRNPYFFRASQIDSSQAKPIAAIFEAFDWGQGVLIHSDDEYGEGFLPS 187
           I+FS T P L    +PYF RA+  DSSQ K IAAI ++F W   V I+ D+E+GEG LP 
Sbjct: 131 ITFSATCPLLTSINSPYFVRATLDDSSQVKAIAAIVKSFGWRNVVAIYVDNEFGEGILPL 190

Query: 188 MRDALGETNIRVAYETAIPQSASDDRIAKELYKLMTMQTRVFVVHMLPELGSRLFAMAKE 247
           + DAL +    V     IPQ A+DD+I KELYKLMTMQTRVFVVHM P LG R F  A+E
Sbjct: 191 LTDALQDVQAFVVNRCLIPQEANDDQILKELYKLMTMQTRVFVVHMPPTLGFRFFQKARE 250

Query: 248 IGMMGSGYVWIITDGMSNFL-SRIDDSSMVAMSGALGVRTYIPRTEKLEAFQMRWRRKFE 307
           IGMM  GYVW++TDG+ N L S    SS+  M G LGVR++IP+++KL+ F++RW + F 
Sbjct: 251 IGMMEEGYVWLLTDGVMNLLKSNERGSSLENMQGVLGVRSHIPKSKKLKNFRLRWEKMFP 310

Query: 308 KE--ISELNIFGLRAYDAIFALAKAVESVGTTEFIFEKSNVS-SKFTDLDNLGVSRNGPR 367
           K+    E+NIF LRAYD+I ALA AVE        ++    S +  T+L  LGVSR GP 
Sbjct: 311 KKGNDEEMNIFALRAYDSITALAMAVEKTNIKSLRYDHPIASGNNKTNLGTLGVSRYGPS 370

Query: 368 LSEALSKTHFKGLAGDFRMVEMKLKSSTYEIINIRHEKNITVVGYWTPENGLTQTLDFMK 427
           L +ALS   F GLAG+F ++  +L+SS +++INI   +   ++G W P NG+       K
Sbjct: 371 LLKALSNVRFNGLAGEFELINGQLESSVFDVINIIGSEE-RIIGLWRPSNGIVNA----K 430

Query: 428 TNSNTSV--TNLSRIIWPGDDPNSFSFPKGWENPTNEKKLRIGIPVKSGVSKFVRARRDP 487
           + + TSV    L  +IWPG    S   PKGW+ PTN K LR+GIPVK G  +FV A+ DP
Sbjct: 431 SKNTTSVLGERLGPVIWPG---KSKDVPKGWQIPTNGKMLRVGIPVKKGFLEFVDAKIDP 490

Query: 488 VTGWTKRTGYSIDIFEAVIKTLPYAVLYEYVPFANATGAMAGSYDDLVNQVYSGVYDAVV 547
           ++     TGY I+IFEAV+K LPY+V+ +Y+ F +       +YD++V QVY+G YDAVV
Sbjct: 491 ISNAMTPTGYCIEIFEAVLKKLPYSVIPKYIAFLSPD----ENYDEMVYQVYTGAYDAVV 550

Query: 548 GDVSIRESRSLHVDFTFPYSESSVSMVVLFRDIKNKKAWLFLKPLTLDLWLTSAFFFAFI 607
           GDV+I  +RSL+VDFT PY+ES VSM+V  +D  NK  W+FL+P +LDLW+T+A FF FI
Sbjct: 551 GDVTIVANRSLYVDFTLPYTESGVSMMVPLKD--NKNTWVFLRPWSLDLWVTTACFFVFI 610

Query: 608 GLVVWILEHRINEDFRGPPSHEIGTSFWFSFSTMVYAQREKVESNLARFVVIVWLFVVFI 667
           G +VWILEHR+N DFRGPP H+IGTSFWF+FSTM +A REKV SNLARFVV+VW FVV +
Sbjct: 611 GFIVWILEHRVNTDFRGPPHHQIGTSFWFAFSTMNFAHREKVVSNLARFVVLVWCFVVLV 670

Query: 668 LTQGYTASLTSLLTVQKLEPTFTDMKQLKEQKLNVGYPHGSFVQALLIAQGFDPSKLVNY 727
           L Q YTA+LTS  TV+ L+PT T+ K L +   N+GY  G+FV+ LL +QGFD S+L  +
Sbjct: 671 LIQSYTANLTSFFTVKLLQPTVTNWKDLIKFNKNIGYQRGTFVRELLKSQGFDESQLKPF 730

Query: 728 NNMEHCGSLLLNRTIAAAFDEIPYLKVLTTTYCTNCTIM 759
            +   C  L  N TI A+FDE+ Y+KV+ +   +  T++
Sbjct: 731 GSAVECDELFSNGTITASFDEVAYIKVILSQNSSKYTMV 755

BLAST of Cucsat.G12823.T5 vs. ExPASy Swiss-Prot
Match: Q9C5V5 (Glutamate receptor 2.8 OS=Arabidopsis thaliana OX=3702 GN=GLR2.8 PE=2 SV=2)

HSP 1 Score: 700.7 bits (1807), Expect = 1.9e-200
Identity = 378/760 (49.74%), Postives = 510/760 (67.11%), Query Frame = 0

Query: 8   STFIIFLSLCFGLRAFSVMADDEAVTVKVGLVLDSDSSIGKMGVSYMEMALSDFYESHRN 67
           +TF+ +  +C  L     +  ++   +KVG+VLD +++  K+ ++ + +ALSDFY+ H N
Sbjct: 8   NTFLSYF-VCLFLLLEVGLGQNQISEIKVGVVLDLNTTFSKICLTSINLALSDFYKDHPN 67

Query: 68  YKTRLALFAKNSMEDVIEATAAAIELINKEEVEAIVGPQNSMQAAFMADLGKKSQVPIIS 127
           Y+TRLAL  ++SM+D ++A+AAA++LI  E+V AI+GP +SMQA FM  L  K+QVP IS
Sbjct: 68  YRTRLALHVRDSMKDTVQASAAALDLIQNEQVSAIIGPIDSMQAKFMIKLANKTQVPTIS 127

Query: 128 FSVTTPSLNPHRNPYFFRASQIDSSQAKPIAAIFEAFDWGQGVLIHSDDEYGEGFLPSMR 187
           FS T+P L   ++ YF R +  DS Q K IAAIFE+F W   V I+ D+E GEG +P + 
Sbjct: 128 FSATSPLLTSIKSDYFVRGTIDDSYQVKAIAAIFESFGWRSVVAIYVDNELGEGIMPYLF 187

Query: 188 DALGETNIRVAYETAIPQSASDDRIAKELYKLMTMQTRVFVVHMLPELGSRLFAMAKEIG 247
           DAL +  +     + IP  A+DD+I KELYKLMT QTRVFVVHM   L SR+F  A EIG
Sbjct: 188 DALQDVQVD---RSVIPSEANDDQILKELYKLMTRQTRVFVVHMASRLASRIFEKATEIG 247

Query: 248 MMGSGYVWIITDGMSNFLSRIDDS-SMVAMSGALGVRTYIPRTEKLEAFQMRWRRKFEKE 307
           MM  GYVW++T+GM++ +  I    S+  + G LGVR+++P+++ LE F++RW+R F+KE
Sbjct: 248 MMEEGYVWLMTNGMTHMMRHIHHGRSLNTIDGVLGVRSHVPKSKGLEDFRLRWKRNFKKE 307

Query: 308 ----ISELNIFGLRAYDAIFALAKAVESVGTTEFIFEK-SNVSSKFTDLDNLGVSRNGPR 367
                 +L+IFGL AYD+  ALA AVE    + F +   S  S+  TDL  L VSR GP 
Sbjct: 308 NPWLRDDLSIFGLWAYDSTTALAMAVEKTNISSFPYNNASGSSNNMTDLGTLHVSRYGPS 367

Query: 368 LSEALSKTHFKGLAGDFRMVEMKLKSSTYEIIN-IRHEKNITVVGYWTPENGLTQTLDFM 427
           L EALS+  F GLAG F +++ +L+S  +EIIN + +E+ I  VG+WTP NGL      +
Sbjct: 368 LLEALSEIRFNGLAGRFNLIDRQLESPKFEIINFVGNEERI--VGFWTPSNGLVN----V 427

Query: 428 KTNSNTSVT--NLSRIIWPGDDPNSFSFPKGWENPTNEKKLRIGIPVKSGVSKFVRARRD 487
            +N  TS T      +IWPG    S   PKGWE PTN KK+++G+PVK G   FV    D
Sbjct: 428 NSNKTTSFTGERFGPLIWPG---KSTIVPKGWEIPTNGKKIKVGVPVKKGFFNFVEVITD 487

Query: 488 PVTGWTKRTGYSIDIFEAVIKTLPYAVLYEYVPFANATGAMAGSYDDLVNQVYSGVYDAV 547
           P+T  T   GY+IDIFEA +K LPY+V+ +Y  F +        YDDLV +V +G  DAV
Sbjct: 488 PITNITTPKGYAIDIFEAALKKLPYSVIPQYYRFESPD----DDYDDLVYKVDNGTLDAV 547

Query: 548 VGDVSIRESRSLHVDFTFPYSESSVSMVVLFRDIKNKKAWLFLKPLTLDLWLTSAFFFAF 607
           VGDV+I   RSL+ DFT PY+ES VSM+V  RD +NK  W+FLKP  LDLW+T+A FF  
Sbjct: 548 VGDVTITAYRSLYADFTLPYTESGVSMMVPVRDNENKNTWVFLKPWGLDLWVTTACFFVL 607

Query: 608 IGLVVWILEHRINEDFRGPPSHEIGTSFWFSFSTMVYAQREKVESNLARFVVIVWLFVVF 667
           IG VVW+ EHR+N DFRGPP H+IGTSFWFSFSTMV+A REKV SNLARFVV+VW FVV 
Sbjct: 608 IGFVVWLFEHRVNTDFRGPPHHQIGTSFWFSFSTMVFAHREKVVSNLARFVVVVWCFVVL 667

Query: 668 ILTQGYTASLTSLLTVQKLEPTFTDMKQLKEQKLNVGYPHGSFVQALLIAQGFDPSKLVN 727
           +LTQ YTA+LTS LTVQ+ +P   ++K L +    VGY HG+FV+  LI +GF+ SKL  
Sbjct: 668 VLTQSYTANLTSFLTVQRFQPAAINVKDLIKNGDYVGYQHGAFVKDFLIKEGFNVSKLKP 727

Query: 728 YNNMEHCGSLLLNRTIAAAFDEIPYLKVLTTTYCTNCTIM 759
           + + E C +LL N +I+AAFDE+ YL+ + + YC+   I+
Sbjct: 728 FGSSEECHALLSNGSISAAFDEVAYLRAILSQYCSKYAIV 750

BLAST of Cucsat.G12823.T5 vs. ExPASy Swiss-Prot
Match: O81078 (Glutamate receptor 2.9 OS=Arabidopsis thaliana OX=3702 GN=GLR2.9 PE=2 SV=1)

HSP 1 Score: 662.9 bits (1709), Expect = 4.4e-189
Identity = 358/756 (47.35%), Postives = 509/756 (67.33%), Query Frame = 0

Query: 8   STFIIFLSLCFGLRAFSVMADDEAVTVKVGLVLDSDSSIGKMGVSYMEMALSDFYESHRN 67
           +TF+ +    F L     +  ++   +KVG+VLD +++  K+ ++ ++MA+SDFY  H N
Sbjct: 5   NTFLSYFVCGFLLMGVG-LGQNQTSEIKVGVVLDLNTTFSKICLTSIKMAVSDFYADHPN 64

Query: 68  YKTRLALFAKNSMEDVIEATAAAIELINKEEVEAIVGPQNSMQAAFMADLGKKSQVPIIS 127
           Y TRL L  ++SMED ++A+AAA++LI  E+V AI+GP NSMQA FM  L  K+QVP I+
Sbjct: 65  YLTRLTLHVRDSMEDTVQASAAALDLIKTEQVSAIIGPINSMQADFMIKLANKTQVPTIT 124

Query: 128 FSVTTPSLNPHRNPYFFRASQIDSSQAKPIAAIFEAFDWGQGVLIHSDDEYGEGFLPSMR 187
           FS T+P L   ++PYF RA+  DSSQ + IA+IF+ F W + V I+ D+E+GEGF+P + 
Sbjct: 125 FSATSPLLTSIKSPYFVRATIDDSSQVRAIASIFKFFRWRRVVAIYVDNEFGEGFMPFLF 184

Query: 188 DALGETNIRVAYETAIPQSASDDRIAKELYKLMTMQTRVFVVHMLPELGSRLFAMAKEIG 247
           DAL +  ++    + IP  A DD I KEL KLM  Q RVFVVHM   L  R+F +A++IG
Sbjct: 185 DALQDVEVK---RSVIPPEAIDDEIQKELRKLMERQARVFVVHMESSLALRVFQIARDIG 244

Query: 248 MMGSGYVWIITDGMSNFLSRIDDS-SMVAMSGALGVRTYIPRTEKLEAFQMRWRRKFEKE 307
           MM  GYVW++T+GM++ +  I++  S+  + G LGVR+++P++++L  F++RW+R FEKE
Sbjct: 245 MMEEGYVWLMTNGMTHMMRHINNGRSLNTIEGVLGVRSHVPKSKELGDFRLRWKRTFEKE 304

Query: 308 ----ISELNIFGLRAYDAIFALAKAVESVGTTEFIFEK-SNVSSKFTDLDNLGVSRNGPR 367
                 +LN+F L AYD+I ALAKAVE   T    ++  S +S   TDL N+GVS  GP 
Sbjct: 305 NPSMRDDLNVFALWAYDSITALAKAVEKANTKSLWYDNGSTLSKNRTDLGNVGVSLYGPS 364

Query: 368 LSEALSKTHFKGLAGDFRMVEMKLKSSTYEIIN-IRHEKNITVVGYWTPENGLTQTLDFM 427
           L +A S+  F GLAG+F++++ +L+S  +EIIN + +E+ I  +G+WTP +GL   +D  
Sbjct: 365 LQKAFSEVRFNGLAGEFKLIDGQLQSPKFEIINFVGNEERI--IGFWTPRDGL---MDAT 424

Query: 428 KTNSNTSVTNLSRIIWPGDDPNSFSFPKGWENPTNEKKLRIGIPVKSGVSKFVRARRDPV 487
            +N  T    L  +IWPG    S   PKGWE P   KKLR+G+P+K G   FV+   +P+
Sbjct: 425 SSNKKT----LGPVIWPG---KSKIVPKGWEIP--GKKLRVGVPMKKGFFDFVKVTINPI 484

Query: 488 TGWTKRTGYSIDIFEAVIKTLPYAVLYEYVPFANATGAMAGSYDDLVNQVYSGVYDAVVG 547
           T     TGY+I+IFEA +K LPY V+ EYV F +       +Y++LV QVY   +DAVVG
Sbjct: 485 TNKKTPTGYAIEIFEAALKELPYLVIPEYVSFES-----PNNYNNLVYQVYDKTWDAVVG 544

Query: 548 DVSIRESRSLHVDFTFPYSESSVSMVVLFRDIKNKKAWLFLKPLTLDLWLTSAFFFAFIG 607
           D++I  +RSL+ DFT P++ES VSM+V  RD +NK  W+FL+P +L+LW+T+  FF FIG
Sbjct: 545 DITITANRSLYADFTLPFTESGVSMMVPVRDNENKDTWVFLEPWSLELWVTTGCFFVFIG 604

Query: 608 LVVWILEHRINEDFRGPPSHEIGTSFWFSFSTMVYAQREKVESNLARFVVIVWLFVVFIL 667
            VVW+ EHR+N DFRGPP ++IGTS WFSFSTMV+A RE V SNLARFVV+VW FVV +L
Sbjct: 605 FVVWLFEHRVNTDFRGPPQYQIGTSLWFSFSTMVFAHRENVVSNLARFVVVVWCFVVLVL 664

Query: 668 TQGYTASLTSLLTVQKLEPTFTDMKQLKEQKLNVGYPHGSFVQALLIAQGFDPSKLVNYN 727
           TQ YTASLTS LTVQ L+PT T++  L + +  VGY  G+FV+ +L+  GF   +L  ++
Sbjct: 665 TQSYTASLTSFLTVQSLQPTVTNVNDLIKNRDCVGYQGGAFVKDILLGLGFHEDQLKPFD 724

Query: 728 NMEHCGSLL---LNRTIAAAFDEIPYLKVLTTTYCT 754
           + +    LL    ++ IAAAFDE+ YLK + +  C+
Sbjct: 725 SAKDADDLLSKGKSKGIAAAFDEVAYLKAILSQSCS 737

BLAST of Cucsat.G12823.T5 vs. ExPASy Swiss-Prot
Match: O04660 (Glutamate receptor 2.1 OS=Arabidopsis thaliana OX=3702 GN=GLR2.1 PE=2 SV=2)

HSP 1 Score: 642.1 bits (1655), Expect = 8.0e-183
Identity = 342/751 (45.54%), Postives = 485/751 (64.58%), Query Frame = 0

Query: 12  IFLSLCFGLRAFSVM---ADDEAVTVKVGLVLDSDSSIGKMGVSYMEMALSDFYESHRNY 71
           + LSL F +  F +    A +    V VG+V D  ++   M +  + M+LSDFY SH   
Sbjct: 7   LVLSLLFFVIVFLMQVGEAQNRITNVNVGIVNDIGTAYSNMTLLCINMSLSDFYSSHPET 66

Query: 72  KTRLALFAKNSMEDVIEATAAAIELINKEEVEAIVGPQNSMQAAFMADLGKKSQVPIISF 131
           +TRL     +S  DV+ A AAA++LI  +EV+AI+GP  SMQA FM ++G+KSQVPI+++
Sbjct: 67  QTRLVTTVVDSKNDVVTAAAAALDLITNKEVKAILGPWTSMQAQFMIEMGQKSQVPIVTY 126

Query: 132 SVTTPSLNPHRNPYFFRASQIDSSQAKPIAAIFEAFDWGQGVLIHSDDEYGEGFLPSMRD 191
           S T+PSL   R+ YFFRA+  DSSQ   I  I + F W +   ++ DD +GEG +P + D
Sbjct: 127 SATSPSLASIRSQYFFRATYDDSSQVHAIKEIIKLFGWREVAPVYVDDTFGEGIMPRLTD 186

Query: 192 ALGETNIRVAYETAIPQSASDDRIAKELYKLMTMQTRVFVVHMLPELGSRLFAMAKEIGM 251
            L E N+R+ Y T I  +A+DD I+ EL ++MT+ TRVFVVH++  L SR FA A EIG+
Sbjct: 187 VLQEINVRIPYRTVISPNATDDEISVELLRMMTLPTRVFVVHLVELLASRFFAKATEIGL 246

Query: 252 MGSGYVWIITDGMSNFLSRIDDSSMVAMSGALGVRTYIPRTEKLEAFQMRWRRKFEKEIS 311
           M  GYVWI+T+ +++ LS ++++ +  M G LGV+TY+PR+++LE F+ RW ++F   IS
Sbjct: 247 MKQGYVWILTNTITDVLSIMNETEIETMQGVLGVKTYVPRSKELENFRSRWTKRF--PIS 306

Query: 312 ELNIFGLRAYDAIFALAKAVESVGTTEFIFEKSNVSSKFTDLDNLGVSRNGPRLSEALSK 371
           +LN++GL AYDA  ALA A+E  GT+   F K +     ++L  LGVS+ GP+L + LS+
Sbjct: 307 DLNVYGLWAYDATTALALAIEEAGTSNLTFVKMDAKRNVSELQGLGVSQYGPKLLQTLSR 366

Query: 372 THFKGLAGDFRMVEMKLKSSTYEIINIRHEKNITVVGYWTPENGLTQTLD---FMKTNSN 431
             F+GLAGDF+ +  +L+ S +EI+N+  +   T +G+W  E GL + +D     KT  +
Sbjct: 367 VRFQGLAGDFQFINGELQPSVFEIVNVNGQGGRT-IGFWMKEYGLFKNVDQKPASKTTFS 426

Query: 432 TSVTNLSRIIWPGDDPNSFSFPKGWENPTNEKKLRIGIPVKSGVSKFVRARRDPVTGWTK 491
           +    L  IIWPGD   + S PKGWE PTN K+L+IG+PV +   +FV+A RDP+T  T 
Sbjct: 427 SWQDRLRPIIWPGD---TTSVPKGWEIPTNGKRLQIGVPVNNTFQQFVKATRDPITNSTI 486

Query: 492 RTGYSIDIFEAVIKTLPYAVLYEYVPFANATGAMAGSYDDLVNQVYSGVYDAVVGDVSIR 551
            +G+SID FEAVI+ +PY + Y+++PF +      G YD LV QVY G YDAVV D +I 
Sbjct: 487 FSGFSIDYFEAVIQAIPYDISYDFIPFQD------GGYDALVYQVYLGKYDAVVADTTIS 546

Query: 552 ESRSLHVDFTFPYSESSVSMVVLFRDIKNKKAWLFLKPLTLDLWLTSAFFFAFIGLVVWI 611
            +RS++VDF+ PY+ S V +VV  +D   + + +FL PLTL LWL S   F  IGLVVW+
Sbjct: 547 SNRSMYVDFSLPYTPSGVGLVVPVKDSVRRSSTIFLMPLTLALWLISLLSFFIIGLVVWV 606

Query: 612 LEHRINEDFRGPPSHEIGTSFWFSFSTMVYAQREKVESNLARFVVIVWLFVVFILTQGYT 671
           LEHR+N DF GP  +++ T FWFSFS MV+A RE+V S  AR VVI+W F+V +LTQ YT
Sbjct: 607 LEHRVNPDFDGPGQYQLSTIFWFSFSIMVFAPRERVLSFWARVVVIIWYFLVLVLTQSYT 666

Query: 672 ASLTSLLTVQKLEPTFTDMKQLKEQKLNVGYPHGSFVQALLIAQGFDPSKLVNYNNMEHC 731
           ASL SLLT Q L PT T++  L  +  +VGY   SF+   L   GF  + LV+Y + EHC
Sbjct: 667 ASLASLLTTQHLHPTVTNINSLLAKGESVGY-QSSFILGRLRDSGFSEASLVSYGSPEHC 726

Query: 732 GSLL----LNRTIAAAFDEIPYLKVLTTTYC 753
            +LL        ++A   E+PY+++    YC
Sbjct: 727 DALLSKGQAEGGVSAVLMEVPYVRIFLGQYC 744

BLAST of Cucsat.G12823.T5 vs. ExPASy Swiss-Prot
Match: Q9LFN5 (Glutamate receptor 2.5 OS=Arabidopsis thaliana OX=3702 GN=GLR2.5 PE=2 SV=2)

HSP 1 Score: 637.9 bits (1644), Expect = 1.5e-181
Identity = 347/758 (45.78%), Postives = 491/758 (64.78%), Query Frame = 0

Query: 10  FIIFLSLCFGLRAFSVMADDEAVTVKVGLVLDSDSSIGKMGVSYMEMALSDFYESHRNYK 69
           F++FL L  G       +  EA+ VKVG+VL S+ ++  + +  + M+LS+FY +H  +K
Sbjct: 19  FLVFLVLSLG------KSQKEALQVKVGIVLGSNVTLADLSLRAINMSLSEFYNTHNGFK 78

Query: 70  TRLALFAKNSMEDVIEATAAAIELINKEEVEAIVGPQNSMQAAFMADLGKKSQVPIISFS 129
           TR+ L  ++S + V+ A A+A+ LI K EV AI+GP  SMQA F+ +LG +S+VPIISFS
Sbjct: 79  TRIVLNVRDSKQTVVGAAASALYLIKKREVVAIIGPGTSMQAPFLINLGNQSKVPIISFS 138

Query: 130 VTTPSLNPHRNPYFFRASQIDSSQAKPIAAIFEAFDWGQGVLIHSDDEYGEGFLPSMRDA 189
            T+P L+  R+PYF RA+  DSSQ + I+AI E+F W + V I+ D+E+GEG LP++ DA
Sbjct: 139 ATSPLLDSLRSPYFIRATHDDSSQVQAISAIIESFRWREVVPIYVDNEFGEGILPNLVDA 198

Query: 190 LGETNIRVAYETAIPQSASDDRIAKELYKLMTMQTRVFVVHMLPELGSRLFAMAKEIGMM 249
             E N+R+ Y +AI    SDD+I KELYKLMTM TRVF+VHMLP+LGSRLF++AKEI M+
Sbjct: 199 FQEINVRIRYRSAISLHYSDDQIKKELYKLMTMPTRVFIVHMLPDLGSRLFSIAKEIDML 258

Query: 250 GSGYVWIITDGMSNFLSRIDDSSMVAMSGALGVRTYIPRTEKLEAFQMRWRRKFEKEISE 309
             GYVWI+T+G+++ +S + +SS+V M G LGV+TY  ++++L   + RW+++F  E  E
Sbjct: 259 SKGYVWIVTNGIADLMSIMGESSLVNMHGVLGVKTYFAKSKELLHLEARWQKRFGGE--E 318

Query: 310 LNIFGLRAYDAIFALAKAVESVGTTEFIF----EKSNVSSKFTDLDNLGVSRNGPRLSEA 369
           LN F   AYDA  ALA +VE +      F    E ++     TDLD LGV+ +GP+L +A
Sbjct: 319 LNNFACWAYDAATALAMSVEEIRHVNMSFNTTKEDTSRDDIGTDLDELGVALSGPKLLDA 378

Query: 370 LSKTHFKGLAGDFRMVEMKLKSSTYEIINIRHEKNITVVGYWTPENGLTQTLDFMKTNSN 429
           LS   FKG+AG F++   KL+++T++IINI  E     VG+W  + GL ++L   K +  
Sbjct: 379 LSTVSFKGVAGRFQLKNGKLEATTFKIINI-EESGERTVGFWKSKVGLVKSLRVDKVSH- 438

Query: 430 TSVTNLSRIIWPGDDPNSFSFPKGWENPTNEKKLRIGIPVKSGVSKFVRARRDPVTGWTK 489
            S   L  IIWPGD   +   PKGWE PTN KKLRI +P K G + FV   +D  T    
Sbjct: 439 -SSRRLRPIIWPGD---TIFVPKGWEFPTNAKKLRIAVPKKDGFNNFVEVTKDENTNVPT 498

Query: 490 RTGYSIDIFEAVIKTLPYAVLYEYVPFANATGAMAGSYDDLVNQVYSGVYDAVVGDVSIR 549
            TG+ ID+F  V+  +PYAV YEY+PF    G   GSYD++V  V+ G +D  VGD +I 
Sbjct: 499 VTGFCIDVFNTVMSQMPYAVSYEYIPFDTPDGKPRGSYDEMVYNVFLGEFDGAVGDTTIL 558

Query: 550 ESRSLHVDFTFPYSESSVSMVVLFRDIKNKKAWLFLKPLTLDLWLTSAFFFAFIGLVVWI 609
            +RS +VDF  PYSE+ +  +V  +D K K  W+FLKPLT +LWL +A  F +IG++VWI
Sbjct: 559 ANRSHYVDFALPYSETGIVFLVPVKDGKEKGEWVFLKPLTKELWLVTAASFLYIGIMVWI 618

Query: 610 LEHRINEDFRGPP-SHEIGTSFWFSFSTMVYAQREKVESNLARFVVIVWLFVVFILTQGY 669
            E++ +E+FR      +I + F+FSFST+ +A R   ES   R +V+VW FV+ ILTQ Y
Sbjct: 619 FEYQADEEFREQMIIDKISSVFYFSFSTLFFAHRRPSESFFTRVLVVVWCFVLLILTQSY 678

Query: 670 TASLTSLLTVQKLEPTFTDMKQLKEQKLNVGYPHGSFVQALLIAQGFDPSKLVNYNNMEH 729
           TA+LTS+LTVQ+L PT   M  L++  +N+GY  GSF    L    FD S+L  YN+ E 
Sbjct: 679 TATLTSMLTVQELRPTVRHMDDLRKSGVNIGYQTGSFTFERLKQMRFDESRLKTYNSPEE 738

Query: 730 CGSLLLNRT----IAAAFDEIPYLKVLTTTYCTNCTIM 759
              L L+++    I AAFDE+ Y+K+    YC+  +I+
Sbjct: 739 MRELFLHKSSNGGIDAAFDEVAYIKLFMAKYCSEYSII 762

BLAST of Cucsat.G12823.T5 vs. NCBI nr
Match: XP_004146855.2 (glutamate receptor 2.7 isoform X1 [Cucumis sativus] >KAE8651296.1 hypothetical protein Csa_001200 [Cucumis sativus])

HSP 1 Score: 1493 bits (3865), Expect = 0.0
Identity = 757/758 (99.87%), Postives = 758/758 (100.00%), Query Frame = 0

Query: 1   MESLSIFSTFIIFLSLCFGLRAFSVMADDEAVTVKVGLVLDSDSSIGKMGVSYMEMALSD 60
           MESLSIFSTFIIFLSLCFGLRAFSVMADDEAVTVKVGLVLDSDSSIGKMGVSYMEMALSD
Sbjct: 83  MESLSIFSTFIIFLSLCFGLRAFSVMADDEAVTVKVGLVLDSDSSIGKMGVSYMEMALSD 142

Query: 61  FYESHRNYKTRLALFAKNSMEDVIEATAAAIELINKEEVEAIVGPQNSMQAAFMADLGKK 120
           FYESHRNYKTRLALFAKNSMEDVIEATAAAIELINKEEVEAIVGPQNSMQAAFMADLGKK
Sbjct: 143 FYESHRNYKTRLALFAKNSMEDVIEATAAAIELINKEEVEAIVGPQNSMQAAFMADLGKK 202

Query: 121 SQVPIISFSVTTPSLNPHRNPYFFRASQIDSSQAKPIAAIFEAFDWGQGVLIHSDDEYGE 180
           SQVPIISFSVTTPSLNPHRNPYFFRASQIDSSQAKPIAAIFEAFDWGQGVLIHSDDEYGE
Sbjct: 203 SQVPIISFSVTTPSLNPHRNPYFFRASQIDSSQAKPIAAIFEAFDWGQGVLIHSDDEYGE 262

Query: 181 GFLPSMRDALGETNIRVAYETAIPQSASDDRIAKELYKLMTMQTRVFVVHMLPELGSRLF 240
           GFLPSMRDALGETNIRVAYETAIPQSASDDRIAKELYKLMTMQTRVFVVHMLPELGSRLF
Sbjct: 263 GFLPSMRDALGETNIRVAYETAIPQSASDDRIAKELYKLMTMQTRVFVVHMLPELGSRLF 322

Query: 241 AMAKEIGMMGSGYVWIITDGMSNFLSRIDDSSMVAMSGALGVRTYIPRTEKLEAFQMRWR 300
           AMAKEIGMMGSGYVWIITDGMSNFLSRIDDSSMVAMSGALGVRTYIPRTEKLEAFQMRWR
Sbjct: 323 AMAKEIGMMGSGYVWIITDGMSNFLSRIDDSSMVAMSGALGVRTYIPRTEKLEAFQMRWR 382

Query: 301 RKFEKEISELNIFGLRAYDAIFALAKAVESVGTTEFIFEKSNVSSKFTDLDNLGVSRNGP 360
           RKFEKEISELNIFGLRAYDAIFALAKAVESVGTTEFIFEKSNVSSKFTDLDNLGVSRNGP
Sbjct: 383 RKFEKEISELNIFGLRAYDAIFALAKAVESVGTTEFIFEKSNVSSKFTDLDNLGVSRNGP 442

Query: 361 RLSEALSKTHFKGLAGDFRMVEMKLKSSTYEIINIRHEKNITVVGYWTPENGLTQTLDFM 420
           RLSEALSKTHFKGLAGDFRMVEMKLKSSTYEIINIRHEKNITVVGYWTPENGLTQTLDFM
Sbjct: 443 RLSEALSKTHFKGLAGDFRMVEMKLKSSTYEIINIRHEKNITVVGYWTPENGLTQTLDFM 502

Query: 421 KTNSNTSVTNLSRIIWPGDDPNSFSFPKGWENPTNEKKLRIGIPVKSGVSKFVRARRDPV 480
           KTNSNTSVTNLSRIIWPGDDPNSFSFPKGWENPTNEKKLRIGIPVKSGVSKFVRARRDPV
Sbjct: 503 KTNSNTSVTNLSRIIWPGDDPNSFSFPKGWENPTNEKKLRIGIPVKSGVSKFVRARRDPV 562

Query: 481 TGWTKRTGYSIDIFEAVIKTLPYAVLYEYVPFANATGAMAGSYDDLVNQVYSGVYDAVVG 540
           TGWTKRTGYSIDIFEAVIKTLPYAVLYEYVPFANATGAMAGSYDDLVNQVYSGVYDAVVG
Sbjct: 563 TGWTKRTGYSIDIFEAVIKTLPYAVLYEYVPFANATGAMAGSYDDLVNQVYSGVYDAVVG 622

Query: 541 DVSIRESRSLHVDFTFPYSESSVSMVVLFRDIKNKKAWLFLKPLTLDLWLTSAFFFAFIG 600
           DVSIRESRSLHVDFTFPYSESSVSMVVLFRDIKNKKAWLFLKPLTLDLWLTSAFFFAFIG
Sbjct: 623 DVSIRESRSLHVDFTFPYSESSVSMVVLFRDIKNKKAWLFLKPLTLDLWLTSAFFFAFIG 682

Query: 601 LVVWILEHRINEDFRGPPSHEIGTSFWFSFSTMVYAQREKVESNLARFVVIVWLFVVFIL 660
           LVVWILEHRINEDFRGPPSHEIGTSFWFSFSTMVYAQREKVESNLARFVVIVWLFVVFIL
Sbjct: 683 LVVWILEHRINEDFRGPPSHEIGTSFWFSFSTMVYAQREKVESNLARFVVIVWLFVVFIL 742

Query: 661 TQGYTASLTSLLTVQKLEPTFTDMKQLKEQKLNVGYPHGSFVQALLIAQGFDPSKLVNYN 720
           TQGYTASLTSLLTVQKLEPTFTDMKQLKEQKLNVGYPHGSFVQALLIAQGFDPSKLVNYN
Sbjct: 743 TQGYTASLTSLLTVQKLEPTFTDMKQLKEQKLNVGYPHGSFVQALLIAQGFDPSKLVNYN 802

Query: 721 NMEHCGSLLLNRTIAAAFDEIPYLKVLTTTYCTNCTIM 758
           NMEHCGSLLLNRTIAAAFDEIPYLKVLTTTYCTNCTI+
Sbjct: 803 NMEHCGSLLLNRTIAAAFDEIPYLKVLTTTYCTNCTIV 840

BLAST of Cucsat.G12823.T5 vs. NCBI nr
Match: XP_008447592.1 (PREDICTED: glutamate receptor 2.7-like [Cucumis melo] >KAA0061099.1 glutamate receptor 2.7-like [Cucumis melo var. makuwa] >TYK03769.1 glutamate receptor 2.7-like [Cucumis melo var. makuwa])

HSP 1 Score: 1379 bits (3568), Expect = 0.0
Identity = 702/758 (92.61%), Postives = 717/758 (94.59%), Query Frame = 0

Query: 1   MESLSIFSTFIIFLSLCFGLRAFSVMADDEAVTVKVGLVLDSDSSIGKMGVSYMEMALSD 60
           MES SIFSTFI FLSLCFGLRAFS     EAVTVKVG+VLDSDSSIGKMG SYMEMALSD
Sbjct: 1   MESRSIFSTFIFFLSLCFGLRAFS-----EAVTVKVGVVLDSDSSIGKMGFSYMEMALSD 60

Query: 61  FYESHRNYKTRLALFAKNSMEDVIEATAAAIELINKEEVEAIVGPQNSMQAAFMADLGKK 120
           FYESHRNYKTRLALFAKNSMEDVIEA AAA+ELI+KEEVEAIVGPQNS QAAFMADLGKK
Sbjct: 61  FYESHRNYKTRLALFAKNSMEDVIEAAAAAMELISKEEVEAIVGPQNSKQAAFMADLGKK 120

Query: 121 SQVPIISFSVTTPSLNPHRNPYFFRASQIDSSQAKPIAAIFEAFDWGQGVLIHSDDEYGE 180
           SQVPIISFS TTPSLNPHRNPYFFRA+Q+DSSQAKPIAAIFEAFDW QGVLIHSDDEYGE
Sbjct: 121 SQVPIISFSATTPSLNPHRNPYFFRATQMDSSQAKPIAAIFEAFDWRQGVLIHSDDEYGE 180

Query: 181 GFLPSMRDALGETNIRVAYETAIPQSASDDRIAKELYKLMTMQTRVFVVHMLPELGSRLF 240
           GFLPSMRDAL ET+ RVAYETAIPQSASDDRIAKELYKLMTMQTRVFVVHMLPELGSRLF
Sbjct: 181 GFLPSMRDALRETSTRVAYETAIPQSASDDRIAKELYKLMTMQTRVFVVHMLPELGSRLF 240

Query: 241 AMAKEIGMMGSGYVWIITDGMSNFLSRIDDSSMVAMSGALGVRTYIPRTEKLEAFQMRWR 300
           AMAKEIGMMGSGYVWIITDGMSNFLS IDDSSMVAMSGALGVRTYIPRTEKLE FQMRWR
Sbjct: 241 AMAKEIGMMGSGYVWIITDGMSNFLSWIDDSSMVAMSGALGVRTYIPRTEKLEVFQMRWR 300

Query: 301 RKFEKEISELNIFGLRAYDAIFALAKAVESVGTTEFIFEKSNVSSKFTDLDNLGVSRNGP 360
           RKFEKEISELNIFGLRAYDAI ALAKAVES GTTEFI EKSNVS K TDLDNLGVSRNGP
Sbjct: 301 RKFEKEISELNIFGLRAYDAIVALAKAVESAGTTEFILEKSNVSGKSTDLDNLGVSRNGP 360

Query: 361 RLSEALSKTHFKGLAGDFRMVEMKLKSSTYEIINIRHEKNITVVGYWTPENGLTQTLDFM 420
           RLSEALSKTHFKGLAGDFRMVE KLKSSTYEIINI HEKNITVVGYWTPENGLTQTLDFM
Sbjct: 361 RLSEALSKTHFKGLAGDFRMVEGKLKSSTYEIININHEKNITVVGYWTPENGLTQTLDFM 420

Query: 421 KTNSNTSVTNLSRIIWPGDDPNSFSFPKGWENPTNEKKLRIGIPVKSGVSKFVRARRDPV 480
           K +SNTSVTNLSRI+WPGD PNSFSFPKGWENPTNEKKLRIGIPVKSGVSKF+R  RDPV
Sbjct: 421 KISSNTSVTNLSRIVWPGDGPNSFSFPKGWENPTNEKKLRIGIPVKSGVSKFIREIRDPV 480

Query: 481 TGWTKRTGYSIDIFEAVIKTLPYAVLYEYVPFANATGAMAGSYDDLVNQVYSGVYDAVVG 540
           TGWTKRTGYSIDIFEAVI TLPYAV YEYVPFANATGAMAGSY++LV QVY GVYDAVVG
Sbjct: 481 TGWTKRTGYSIDIFEAVINTLPYAVFYEYVPFANATGAMAGSYEELVKQVYFGVYDAVVG 540

Query: 541 DVSIRESRSLHVDFTFPYSESSVSMVVLFRDIKNKKAWLFLKPLTLDLWLTSAFFFAFIG 600
           DVSIRESRSL+ DFT PYSESSVSMVVLFRD KNKKAWLFLKPLTLDLWLTSAFFFAFIG
Sbjct: 541 DVSIRESRSLYADFTLPYSESSVSMVVLFRDNKNKKAWLFLKPLTLDLWLTSAFFFAFIG 600

Query: 601 LVVWILEHRINEDFRGPPSHEIGTSFWFSFSTMVYAQREKVESNLARFVVIVWLFVVFIL 660
           LVVWILEHRINEDFRGPPSH+IGTSFWFSFSTMVYAQREKVESNLARFVVIVWLFVV IL
Sbjct: 601 LVVWILEHRINEDFRGPPSHQIGTSFWFSFSTMVYAQREKVESNLARFVVIVWLFVVLIL 660

Query: 661 TQGYTASLTSLLTVQKLEPTFTDMKQLKEQKLNVGYPHGSFVQALLIAQGFDPSKLVNYN 720
           TQ YTASLTSLLTVQKLEPTF DM QLKEQKLNVGYPHGSFVQALLIA+GFDPSKLVNYN
Sbjct: 661 TQSYTASLTSLLTVQKLEPTFADMNQLKEQKLNVGYPHGSFVQALLIAEGFDPSKLVNYN 720

Query: 721 NMEHCGSLLLNRTIAAAFDEIPYLKVLTTTYCTNCTIM 758
           NM HCGSL LN TIAAAFDEIPYLKVLTTTYCTNCTI+
Sbjct: 721 NMAHCGSLFLNGTIAAAFDEIPYLKVLTTTYCTNCTIV 753

BLAST of Cucsat.G12823.T5 vs. NCBI nr
Match: XP_031738896.1 (glutamate receptor 2.7 isoform X2 [Cucumis sativus])

HSP 1 Score: 1378 bits (3566), Expect = 0.0
Identity = 711/758 (93.80%), Postives = 712/758 (93.93%), Query Frame = 0

Query: 1   MESLSIFSTFIIFLSLCFGLRAFSVMADDEAVTVKVGLVLDSDSSIGKMGVSYMEMALSD 60
           MESLSIFSTFIIFLSLCFGLRAFSVMADDEAVTVKVGLVLDSDSSIGKMGVSYMEMALSD
Sbjct: 83  MESLSIFSTFIIFLSLCFGLRAFSVMADDEAVTVKVGLVLDSDSSIGKMGVSYMEMALSD 142

Query: 61  FYESHRNYKTRLALFAKNSMEDVIEATAAAIELINKEEVEAIVGPQNSMQAAFMADLGKK 120
           FYESHRNYKTRLALFAKNSMEDVIEATAAAIELINKEEVEAIVGPQNSMQAAFMADLGKK
Sbjct: 143 FYESHRNYKTRLALFAKNSMEDVIEATAAAIELINKEEVEAIVGPQNSMQAAFMADLGKK 202

Query: 121 SQVPIISFSVTTPSLNPHRNPYFFRASQIDSSQAKPIAAIFEAFDWGQGVLIHSDDEYGE 180
           SQ                                              GVLIHSDDEYGE
Sbjct: 203 SQ----------------------------------------------GVLIHSDDEYGE 262

Query: 181 GFLPSMRDALGETNIRVAYETAIPQSASDDRIAKELYKLMTMQTRVFVVHMLPELGSRLF 240
           GFLPSMRDALGETNIRVAYETAIPQSASDDRIAKELYKLMTMQTRVFVVHMLPELGSRLF
Sbjct: 263 GFLPSMRDALGETNIRVAYETAIPQSASDDRIAKELYKLMTMQTRVFVVHMLPELGSRLF 322

Query: 241 AMAKEIGMMGSGYVWIITDGMSNFLSRIDDSSMVAMSGALGVRTYIPRTEKLEAFQMRWR 300
           AMAKEIGMMGSGYVWIITDGMSNFLSRIDDSSMVAMSGALGVRTYIPRTEKLEAFQMRWR
Sbjct: 323 AMAKEIGMMGSGYVWIITDGMSNFLSRIDDSSMVAMSGALGVRTYIPRTEKLEAFQMRWR 382

Query: 301 RKFEKEISELNIFGLRAYDAIFALAKAVESVGTTEFIFEKSNVSSKFTDLDNLGVSRNGP 360
           RKFEKEISELNIFGLRAYDAIFALAKAVESVGTTEFIFEKSNVSSKFTDLDNLGVSRNGP
Sbjct: 383 RKFEKEISELNIFGLRAYDAIFALAKAVESVGTTEFIFEKSNVSSKFTDLDNLGVSRNGP 442

Query: 361 RLSEALSKTHFKGLAGDFRMVEMKLKSSTYEIINIRHEKNITVVGYWTPENGLTQTLDFM 420
           RLSEALSKTHFKGLAGDFRMVEMKLKSSTYEIINIRHEKNITVVGYWTPENGLTQTLDFM
Sbjct: 443 RLSEALSKTHFKGLAGDFRMVEMKLKSSTYEIINIRHEKNITVVGYWTPENGLTQTLDFM 502

Query: 421 KTNSNTSVTNLSRIIWPGDDPNSFSFPKGWENPTNEKKLRIGIPVKSGVSKFVRARRDPV 480
           KTNSNTSVTNLSRIIWPGDDPNSFSFPKGWENPTNEKKLRIGIPVKSGVSKFVRARRDPV
Sbjct: 503 KTNSNTSVTNLSRIIWPGDDPNSFSFPKGWENPTNEKKLRIGIPVKSGVSKFVRARRDPV 562

Query: 481 TGWTKRTGYSIDIFEAVIKTLPYAVLYEYVPFANATGAMAGSYDDLVNQVYSGVYDAVVG 540
           TGWTKRTGYSIDIFEAVIKTLPYAVLYEYVPFANATGAMAGSYDDLVNQVYSGVYDAVVG
Sbjct: 563 TGWTKRTGYSIDIFEAVIKTLPYAVLYEYVPFANATGAMAGSYDDLVNQVYSGVYDAVVG 622

Query: 541 DVSIRESRSLHVDFTFPYSESSVSMVVLFRDIKNKKAWLFLKPLTLDLWLTSAFFFAFIG 600
           DVSIRESRSLHVDFTFPYSESSVSMVVLFRDIKNKKAWLFLKPLTLDLWLTSAFFFAFIG
Sbjct: 623 DVSIRESRSLHVDFTFPYSESSVSMVVLFRDIKNKKAWLFLKPLTLDLWLTSAFFFAFIG 682

Query: 601 LVVWILEHRINEDFRGPPSHEIGTSFWFSFSTMVYAQREKVESNLARFVVIVWLFVVFIL 660
           LVVWILEHRINEDFRGPPSHEIGTSFWFSFSTMVYAQREKVESNLARFVVIVWLFVVFIL
Sbjct: 683 LVVWILEHRINEDFRGPPSHEIGTSFWFSFSTMVYAQREKVESNLARFVVIVWLFVVFIL 742

Query: 661 TQGYTASLTSLLTVQKLEPTFTDMKQLKEQKLNVGYPHGSFVQALLIAQGFDPSKLVNYN 720
           TQGYTASLTSLLTVQKLEPTFTDMKQLKEQKLNVGYPHGSFVQALLIAQGFDPSKLVNYN
Sbjct: 743 TQGYTASLTSLLTVQKLEPTFTDMKQLKEQKLNVGYPHGSFVQALLIAQGFDPSKLVNYN 794

Query: 721 NMEHCGSLLLNRTIAAAFDEIPYLKVLTTTYCTNCTIM 758
           NMEHCGSLLLNRTIAAAFDEIPYLKVLTTTYCTNCTI+
Sbjct: 803 NMEHCGSLLLNRTIAAAFDEIPYLKVLTTTYCTNCTIV 794

BLAST of Cucsat.G12823.T5 vs. NCBI nr
Match: XP_038898636.1 (glutamate receptor 2.7-like [Benincasa hispida])

HSP 1 Score: 1317 bits (3409), Expect = 0.0
Identity = 673/758 (88.79%), Postives = 701/758 (92.48%), Query Frame = 0

Query: 1   MESLSIFSTFIIFLSLCFGLRAFSVMADDEAVTVKVGLVLDSDSSIGKMGVSYMEMALSD 60
           MES ++FSTFI FLSLCFGLRAFS MADDEAVTVKVG+VL+SDSSIGKMG S MEMALSD
Sbjct: 1   MESHTVFSTFIFFLSLCFGLRAFSAMADDEAVTVKVGVVLNSDSSIGKMGFSCMEMALSD 60

Query: 61  FYESHRNYKTRLALFAKNSMEDVIEATAAAIELINKEEVEAIVGPQNSMQAAFMADLGKK 120
           FY  HRNYKTRLALF KNSMEDVIEATAAA+ELI KEEVEAIVGPQ+S+QAAFMADLGKK
Sbjct: 61  FYGFHRNYKTRLALFPKNSMEDVIEATAAAMELIKKEEVEAIVGPQDSIQAAFMADLGKK 120

Query: 121 SQVPIISFSVTTPSLNPHRNPYFFRASQIDSSQAKPIAAIFEAFDWGQGVLIHSDDEYGE 180
           SQVPIISFS TTPSLN H NPYFFRA+QIDSSQAKPIAAIFEAF+W Q VLIHSDDEYGE
Sbjct: 121 SQVPIISFSATTPSLNFHWNPYFFRATQIDSSQAKPIAAIFEAFEWRQAVLIHSDDEYGE 180

Query: 181 GFLPSMRDALGETNIRVAYETAIPQSASDDRIAKELYKLMTMQTRVFVVHMLPELGSRLF 240
           GFL SMRDAL E N RVAYETAIPQSA DDRIAKELYKLMTMQTRVFVVHM PELGSR+F
Sbjct: 181 GFLSSMRDALREINTRVAYETAIPQSAFDDRIAKELYKLMTMQTRVFVVHMSPELGSRVF 240

Query: 241 AMAKEIGMMGSGYVWIITDGMSNFLSRIDDSSMVAMSGALGVRTYIPRTEKLEAFQMRWR 300
           AMAKEIGMM SGY WIITDGMSNFLSRIDDSSM AMSGALGVRTYIPRTEKLE FQMRWR
Sbjct: 241 AMAKEIGMMESGYAWIITDGMSNFLSRIDDSSMEAMSGALGVRTYIPRTEKLEGFQMRWR 300

Query: 301 RKFEKEISELNIFGLRAYDAIFALAKAVESVGTTEFIFEKSNVSSKFTDLDNLGVSRNGP 360
           +KFEKE SELNIFGLRAYDA  ALAKAVE VGTTEFIFEKSNVS K TDLDNLGVSRNGP
Sbjct: 301 KKFEKEFSELNIFGLRAYDATVALAKAVEKVGTTEFIFEKSNVSGKSTDLDNLGVSRNGP 360

Query: 361 RLSEALSKTHFKGLAGDFRMVEMKLKSSTYEIINIRHEKNITVVGYWTPENGLTQTLDFM 420
            LSEALSKTHFKGLAGDFRMVE KLKSSTYEIINI HEKNITVVGYWTPENGL+QTLD  
Sbjct: 361 MLSEALSKTHFKGLAGDFRMVEGKLKSSTYEIININHEKNITVVGYWTPENGLSQTLDST 420

Query: 421 KTNSNTSVTNLSRIIWPGDDPNSFSFPKGWENPTNEKKLRIGIPVKSGVSKFVRARRDPV 480
           K +SNTSV+NLSRIIWPGDDPN+FSFPKGWE PTNEKKLRIGIPVKSGVSKFVR  RDPV
Sbjct: 421 KMSSNTSVSNLSRIIWPGDDPNTFSFPKGWEIPTNEKKLRIGIPVKSGVSKFVREIRDPV 480

Query: 481 TGWTKRTGYSIDIFEAVIKTLPYAVLYEYVPFANATGAMAGSYDDLVNQVYSGVYDAVVG 540
           TG  KRTGYSIDIFEAVI T+PYAV YEY+ FA+A GAMAGSY+DLV QVY GVYDAVVG
Sbjct: 481 TGRPKRTGYSIDIFEAVIDTMPYAVSYEYIQFADAEGAMAGSYEDLVKQVYFGVYDAVVG 540

Query: 541 DVSIRESRSLHVDFTFPYSESSVSMVVLFRDIKNKKAWLFLKPLTLDLWLTSAFFFAFIG 600
           D+SIRESRSL+VDFT PYSESSVSMVVLFRD KNKKAWLFLKPLTL+LWLTSAFFFAFIG
Sbjct: 541 DLSIRESRSLYVDFTLPYSESSVSMVVLFRDNKNKKAWLFLKPLTLNLWLTSAFFFAFIG 600

Query: 601 LVVWILEHRINEDFRGPPSHEIGTSFWFSFSTMVYAQREKVESNLARFVVIVWLFVVFIL 660
           LV+WILEHRINEDFRGPPSHEIGTSFWFSFSTMVYAQREKVESNLARFVV+VWLFVV IL
Sbjct: 601 LVIWILEHRINEDFRGPPSHEIGTSFWFSFSTMVYAQREKVESNLARFVVVVWLFVVLIL 660

Query: 661 TQGYTASLTSLLTVQKLEPTFTDMKQLKEQKLNVGYPHGSFVQALLIAQGFDPSKLVNYN 720
           TQ YTASLTSLLTVQKLEPTFTDM QLKEQK+NVGYP+GSFVQALLIA+GFDPSKLV YN
Sbjct: 661 TQSYTASLTSLLTVQKLEPTFTDMNQLKEQKVNVGYPNGSFVQALLIAEGFDPSKLVIYN 720

Query: 721 NMEHCGSLLLNRTIAAAFDEIPYLKVLTTTYCTNCTIM 758
           NM HCGSL LN TI AAFDEIPYLKVLTTTYCTNCTI+
Sbjct: 721 NMAHCGSLFLNGTITAAFDEIPYLKVLTTTYCTNCTIV 758

BLAST of Cucsat.G12823.T5 vs. NCBI nr
Match: XP_023523601.1 (glutamate receptor 2.7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1196 bits (3094), Expect = 0.0
Identity = 611/752 (81.25%), Postives = 664/752 (88.30%), Query Frame = 0

Query: 10  FIIFLSLCFGLRAFSVMADDEAVTVKVGLVLDSDSSIGKMGVSYMEMALSDFYESHRNYK 69
           F  FL+LCFGLRA S MADDEAV VKVG+VLDS+S IGKMG+S+M+MALSDFYE H+NY 
Sbjct: 8   FFFFLALCFGLRALSAMADDEAVKVKVGVVLDSNSLIGKMGLSFMDMALSDFYEFHKNYN 67

Query: 70  TRLALFAKNSMEDVIEATAAAIELINKEEVEAIVGPQNSMQAAFMADLGKKSQVPIISFS 129
           TRL LF KNSM D +EATAAA+ELINKEEVEAIVGPQ+S QAAFMADLGKKS VPIISFS
Sbjct: 68  TRLTLFPKNSMGDDLEATAAALELINKEEVEAIVGPQSSSQAAFMADLGKKSHVPIISFS 127

Query: 130 VTTP--SLNPHRNPYFFRASQIDSSQAKPIAAIFEAFDWGQGVLIHSDDEYGEGFLPSMR 189
            TT   SL+P RNPYFFRA+QIDSSQ KPIA+IF+ F+W Q V+IHSDD+YGEG LPSMR
Sbjct: 128 ATTTMQSLSPRRNPYFFRATQIDSSQVKPIASIFKEFNWRQAVIIHSDDQYGEGILPSMR 187

Query: 190 DALGETNIRVAYETAIPQSASDDRIAKELYKLMTMQTRVFVVHMLPELGSRLFAMAKEIG 249
           DAL E N  VA ETAIPQSASDDRIAK+LY+LMTMQTRVFVVHM PELGSR+FAMAKEIG
Sbjct: 188 DALREINTHVADETAIPQSASDDRIAKQLYRLMTMQTRVFVVHMSPELGSRVFAMAKEIG 247

Query: 250 MMGSGYVWIITDGMSNFLSRIDDSSMVAMSGALGVRTYIPRTEKLEAFQMRWRRKFEKEI 309
           MMGSGY+WIITDGMSNFLS IDDS+M AMSGAL VRTYIP TEKLE FQ RWRR FEKEI
Sbjct: 248 MMGSGYIWIITDGMSNFLSEIDDSAMEAMSGALAVRTYIPITEKLEDFQRRWRRDFEKEI 307

Query: 310 SELNIFGLRAYDAIFALAKAVESVGTTEFIFEKSN-VSSKFTDLDNLGVSRNGPRLSEAL 369
           SELNIFGLRAYDAIFALAKAVE VGTTEFIF+ SN VS K TDLDNLGVSRNGPRLSEAL
Sbjct: 308 SELNIFGLRAYDAIFALAKAVERVGTTEFIFKNSNAVSGKSTDLDNLGVSRNGPRLSEAL 367

Query: 370 SKTHFKGLAGDFRMVEMKLKSSTYEIINIRHEKNITVVGYWTPENGLTQTLDFMKTNSNT 429
           SKTHFKG+AGDFRMVE +LKSSTYEIINI H+KNIT VG+WTPENGLTQTL+  K +SNT
Sbjct: 368 SKTHFKGIAGDFRMVEGQLKSSTYEIININHQKNITTVGFWTPENGLTQTLNSTKMSSNT 427

Query: 430 SVTNLSRIIWPGDDPNSFSFPKGWENPTNEKKLRIGIPVKSGVSKFVRARRDPVTGWTKR 489
           S  NLSRIIWPGD   S S PKGW +P NEKKLRIGIPVKSGVSKF+R  RDP++  TK+
Sbjct: 428 SAANLSRIIWPGD---SSSSPKGWVSPINEKKLRIGIPVKSGVSKFIREIRDPLSSNTKK 487

Query: 490 TGYSIDIFEAVIKTLPYAVLYEYVPFANATGAMAGSYDDLVNQVYSGVYDAVVGDVSIRE 549
           TGYSIDIFEAV++TLPYA+ YEY+ +AN  G MAGSYDDL+ QV  GVYDAVVGD+SIRE
Sbjct: 488 TGYSIDIFEAVVETLPYALNYEYIRYANDVGVMAGSYDDLIKQVQRGVYDAVVGDISIRE 547

Query: 550 SRSLHVDFTFPYSESSVSMVVLFRDIKNKKAWLFLKPLTLDLWLTSAFFFAFIGLVVWIL 609
           SRS +VDFT PYSE+SVSMVVL++D  NKKAWLFLKPLTLDLWLTS FFF FIGLV+W L
Sbjct: 548 SRSSYVDFTLPYSEASVSMVVLYQD-NNKKAWLFLKPLTLDLWLTSGFFFVFIGLVIWTL 607

Query: 610 EHRINEDFRGPPSHEIGTSFWFSFSTMVYAQREKVESNLARFVVIVWLFVVFILTQGYTA 669
           EHRINEDFRGPPSHEIGTSFWF+FSTMVYAQREKVESNLARFVVIVWLFVV ILTQ YTA
Sbjct: 608 EHRINEDFRGPPSHEIGTSFWFAFSTMVYAQREKVESNLARFVVIVWLFVVLILTQSYTA 667

Query: 670 SLTSLLTVQKLEPTFTDMKQLKEQKLNVGYPHGSFVQALLIAQGFDPSKLVNYNNMEHCG 729
           SLTSLLTVQKLEPTF DMKQLKE+K+NVGYP+GSFV  LLI +GFD SKLV YNNM HCG
Sbjct: 668 SLTSLLTVQKLEPTFNDMKQLKEKKVNVGYPNGSFVLDLLITEGFDRSKLVIYNNMAHCG 727

Query: 730 SLLLNRTIAAAFDEIPYLKVLTTTYCTNCTIM 758
           SL LN TIAAAFDE+PYLK LTTTYCTNCTI+
Sbjct: 728 SLFLNGTIAAAFDEVPYLKDLTTTYCTNCTIV 755

BLAST of Cucsat.G12823.T5 vs. ExPASy TrEMBL
Match: A0A5A7V5V8 (Glutamate receptor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold863G001520 PE=3 SV=1)

HSP 1 Score: 1379 bits (3568), Expect = 0.0
Identity = 702/758 (92.61%), Postives = 717/758 (94.59%), Query Frame = 0

Query: 1   MESLSIFSTFIIFLSLCFGLRAFSVMADDEAVTVKVGLVLDSDSSIGKMGVSYMEMALSD 60
           MES SIFSTFI FLSLCFGLRAFS     EAVTVKVG+VLDSDSSIGKMG SYMEMALSD
Sbjct: 1   MESRSIFSTFIFFLSLCFGLRAFS-----EAVTVKVGVVLDSDSSIGKMGFSYMEMALSD 60

Query: 61  FYESHRNYKTRLALFAKNSMEDVIEATAAAIELINKEEVEAIVGPQNSMQAAFMADLGKK 120
           FYESHRNYKTRLALFAKNSMEDVIEA AAA+ELI+KEEVEAIVGPQNS QAAFMADLGKK
Sbjct: 61  FYESHRNYKTRLALFAKNSMEDVIEAAAAAMELISKEEVEAIVGPQNSKQAAFMADLGKK 120

Query: 121 SQVPIISFSVTTPSLNPHRNPYFFRASQIDSSQAKPIAAIFEAFDWGQGVLIHSDDEYGE 180
           SQVPIISFS TTPSLNPHRNPYFFRA+Q+DSSQAKPIAAIFEAFDW QGVLIHSDDEYGE
Sbjct: 121 SQVPIISFSATTPSLNPHRNPYFFRATQMDSSQAKPIAAIFEAFDWRQGVLIHSDDEYGE 180

Query: 181 GFLPSMRDALGETNIRVAYETAIPQSASDDRIAKELYKLMTMQTRVFVVHMLPELGSRLF 240
           GFLPSMRDAL ET+ RVAYETAIPQSASDDRIAKELYKLMTMQTRVFVVHMLPELGSRLF
Sbjct: 181 GFLPSMRDALRETSTRVAYETAIPQSASDDRIAKELYKLMTMQTRVFVVHMLPELGSRLF 240

Query: 241 AMAKEIGMMGSGYVWIITDGMSNFLSRIDDSSMVAMSGALGVRTYIPRTEKLEAFQMRWR 300
           AMAKEIGMMGSGYVWIITDGMSNFLS IDDSSMVAMSGALGVRTYIPRTEKLE FQMRWR
Sbjct: 241 AMAKEIGMMGSGYVWIITDGMSNFLSWIDDSSMVAMSGALGVRTYIPRTEKLEVFQMRWR 300

Query: 301 RKFEKEISELNIFGLRAYDAIFALAKAVESVGTTEFIFEKSNVSSKFTDLDNLGVSRNGP 360
           RKFEKEISELNIFGLRAYDAI ALAKAVES GTTEFI EKSNVS K TDLDNLGVSRNGP
Sbjct: 301 RKFEKEISELNIFGLRAYDAIVALAKAVESAGTTEFILEKSNVSGKSTDLDNLGVSRNGP 360

Query: 361 RLSEALSKTHFKGLAGDFRMVEMKLKSSTYEIINIRHEKNITVVGYWTPENGLTQTLDFM 420
           RLSEALSKTHFKGLAGDFRMVE KLKSSTYEIINI HEKNITVVGYWTPENGLTQTLDFM
Sbjct: 361 RLSEALSKTHFKGLAGDFRMVEGKLKSSTYEIININHEKNITVVGYWTPENGLTQTLDFM 420

Query: 421 KTNSNTSVTNLSRIIWPGDDPNSFSFPKGWENPTNEKKLRIGIPVKSGVSKFVRARRDPV 480
           K +SNTSVTNLSRI+WPGD PNSFSFPKGWENPTNEKKLRIGIPVKSGVSKF+R  RDPV
Sbjct: 421 KISSNTSVTNLSRIVWPGDGPNSFSFPKGWENPTNEKKLRIGIPVKSGVSKFIREIRDPV 480

Query: 481 TGWTKRTGYSIDIFEAVIKTLPYAVLYEYVPFANATGAMAGSYDDLVNQVYSGVYDAVVG 540
           TGWTKRTGYSIDIFEAVI TLPYAV YEYVPFANATGAMAGSY++LV QVY GVYDAVVG
Sbjct: 481 TGWTKRTGYSIDIFEAVINTLPYAVFYEYVPFANATGAMAGSYEELVKQVYFGVYDAVVG 540

Query: 541 DVSIRESRSLHVDFTFPYSESSVSMVVLFRDIKNKKAWLFLKPLTLDLWLTSAFFFAFIG 600
           DVSIRESRSL+ DFT PYSESSVSMVVLFRD KNKKAWLFLKPLTLDLWLTSAFFFAFIG
Sbjct: 541 DVSIRESRSLYADFTLPYSESSVSMVVLFRDNKNKKAWLFLKPLTLDLWLTSAFFFAFIG 600

Query: 601 LVVWILEHRINEDFRGPPSHEIGTSFWFSFSTMVYAQREKVESNLARFVVIVWLFVVFIL 660
           LVVWILEHRINEDFRGPPSH+IGTSFWFSFSTMVYAQREKVESNLARFVVIVWLFVV IL
Sbjct: 601 LVVWILEHRINEDFRGPPSHQIGTSFWFSFSTMVYAQREKVESNLARFVVIVWLFVVLIL 660

Query: 661 TQGYTASLTSLLTVQKLEPTFTDMKQLKEQKLNVGYPHGSFVQALLIAQGFDPSKLVNYN 720
           TQ YTASLTSLLTVQKLEPTF DM QLKEQKLNVGYPHGSFVQALLIA+GFDPSKLVNYN
Sbjct: 661 TQSYTASLTSLLTVQKLEPTFADMNQLKEQKLNVGYPHGSFVQALLIAEGFDPSKLVNYN 720

Query: 721 NMEHCGSLLLNRTIAAAFDEIPYLKVLTTTYCTNCTIM 758
           NM HCGSL LN TIAAAFDEIPYLKVLTTTYCTNCTI+
Sbjct: 721 NMAHCGSLFLNGTIAAAFDEIPYLKVLTTTYCTNCTIV 753

BLAST of Cucsat.G12823.T5 vs. ExPASy TrEMBL
Match: A0A1S3BHT8 (Glutamate receptor OS=Cucumis melo OX=3656 GN=LOC103490007 PE=3 SV=1)

HSP 1 Score: 1379 bits (3568), Expect = 0.0
Identity = 702/758 (92.61%), Postives = 717/758 (94.59%), Query Frame = 0

Query: 1   MESLSIFSTFIIFLSLCFGLRAFSVMADDEAVTVKVGLVLDSDSSIGKMGVSYMEMALSD 60
           MES SIFSTFI FLSLCFGLRAFS     EAVTVKVG+VLDSDSSIGKMG SYMEMALSD
Sbjct: 1   MESRSIFSTFIFFLSLCFGLRAFS-----EAVTVKVGVVLDSDSSIGKMGFSYMEMALSD 60

Query: 61  FYESHRNYKTRLALFAKNSMEDVIEATAAAIELINKEEVEAIVGPQNSMQAAFMADLGKK 120
           FYESHRNYKTRLALFAKNSMEDVIEA AAA+ELI+KEEVEAIVGPQNS QAAFMADLGKK
Sbjct: 61  FYESHRNYKTRLALFAKNSMEDVIEAAAAAMELISKEEVEAIVGPQNSKQAAFMADLGKK 120

Query: 121 SQVPIISFSVTTPSLNPHRNPYFFRASQIDSSQAKPIAAIFEAFDWGQGVLIHSDDEYGE 180
           SQVPIISFS TTPSLNPHRNPYFFRA+Q+DSSQAKPIAAIFEAFDW QGVLIHSDDEYGE
Sbjct: 121 SQVPIISFSATTPSLNPHRNPYFFRATQMDSSQAKPIAAIFEAFDWRQGVLIHSDDEYGE 180

Query: 181 GFLPSMRDALGETNIRVAYETAIPQSASDDRIAKELYKLMTMQTRVFVVHMLPELGSRLF 240
           GFLPSMRDAL ET+ RVAYETAIPQSASDDRIAKELYKLMTMQTRVFVVHMLPELGSRLF
Sbjct: 181 GFLPSMRDALRETSTRVAYETAIPQSASDDRIAKELYKLMTMQTRVFVVHMLPELGSRLF 240

Query: 241 AMAKEIGMMGSGYVWIITDGMSNFLSRIDDSSMVAMSGALGVRTYIPRTEKLEAFQMRWR 300
           AMAKEIGMMGSGYVWIITDGMSNFLS IDDSSMVAMSGALGVRTYIPRTEKLE FQMRWR
Sbjct: 241 AMAKEIGMMGSGYVWIITDGMSNFLSWIDDSSMVAMSGALGVRTYIPRTEKLEVFQMRWR 300

Query: 301 RKFEKEISELNIFGLRAYDAIFALAKAVESVGTTEFIFEKSNVSSKFTDLDNLGVSRNGP 360
           RKFEKEISELNIFGLRAYDAI ALAKAVES GTTEFI EKSNVS K TDLDNLGVSRNGP
Sbjct: 301 RKFEKEISELNIFGLRAYDAIVALAKAVESAGTTEFILEKSNVSGKSTDLDNLGVSRNGP 360

Query: 361 RLSEALSKTHFKGLAGDFRMVEMKLKSSTYEIINIRHEKNITVVGYWTPENGLTQTLDFM 420
           RLSEALSKTHFKGLAGDFRMVE KLKSSTYEIINI HEKNITVVGYWTPENGLTQTLDFM
Sbjct: 361 RLSEALSKTHFKGLAGDFRMVEGKLKSSTYEIININHEKNITVVGYWTPENGLTQTLDFM 420

Query: 421 KTNSNTSVTNLSRIIWPGDDPNSFSFPKGWENPTNEKKLRIGIPVKSGVSKFVRARRDPV 480
           K +SNTSVTNLSRI+WPGD PNSFSFPKGWENPTNEKKLRIGIPVKSGVSKF+R  RDPV
Sbjct: 421 KISSNTSVTNLSRIVWPGDGPNSFSFPKGWENPTNEKKLRIGIPVKSGVSKFIREIRDPV 480

Query: 481 TGWTKRTGYSIDIFEAVIKTLPYAVLYEYVPFANATGAMAGSYDDLVNQVYSGVYDAVVG 540
           TGWTKRTGYSIDIFEAVI TLPYAV YEYVPFANATGAMAGSY++LV QVY GVYDAVVG
Sbjct: 481 TGWTKRTGYSIDIFEAVINTLPYAVFYEYVPFANATGAMAGSYEELVKQVYFGVYDAVVG 540

Query: 541 DVSIRESRSLHVDFTFPYSESSVSMVVLFRDIKNKKAWLFLKPLTLDLWLTSAFFFAFIG 600
           DVSIRESRSL+ DFT PYSESSVSMVVLFRD KNKKAWLFLKPLTLDLWLTSAFFFAFIG
Sbjct: 541 DVSIRESRSLYADFTLPYSESSVSMVVLFRDNKNKKAWLFLKPLTLDLWLTSAFFFAFIG 600

Query: 601 LVVWILEHRINEDFRGPPSHEIGTSFWFSFSTMVYAQREKVESNLARFVVIVWLFVVFIL 660
           LVVWILEHRINEDFRGPPSH+IGTSFWFSFSTMVYAQREKVESNLARFVVIVWLFVV IL
Sbjct: 601 LVVWILEHRINEDFRGPPSHQIGTSFWFSFSTMVYAQREKVESNLARFVVIVWLFVVLIL 660

Query: 661 TQGYTASLTSLLTVQKLEPTFTDMKQLKEQKLNVGYPHGSFVQALLIAQGFDPSKLVNYN 720
           TQ YTASLTSLLTVQKLEPTF DM QLKEQKLNVGYPHGSFVQALLIA+GFDPSKLVNYN
Sbjct: 661 TQSYTASLTSLLTVQKLEPTFADMNQLKEQKLNVGYPHGSFVQALLIAEGFDPSKLVNYN 720

Query: 721 NMEHCGSLLLNRTIAAAFDEIPYLKVLTTTYCTNCTIM 758
           NM HCGSL LN TIAAAFDEIPYLKVLTTTYCTNCTI+
Sbjct: 721 NMAHCGSLFLNGTIAAAFDEIPYLKVLTTTYCTNCTIV 753

BLAST of Cucsat.G12823.T5 vs. ExPASy TrEMBL
Match: A0A6J1D4Y3 (Glutamate receptor OS=Momordica charantia OX=3673 GN=LOC111016979 PE=3 SV=1)

HSP 1 Score: 1195 bits (3092), Expect = 0.0
Identity = 612/757 (80.85%), Postives = 672/757 (88.77%), Query Frame = 0

Query: 1   MESLSIFSTFIIFLSLCFGLRAFSVMADDEAV-TVKVGLVLDSDSSIGKMGVSYMEMALS 60
           ME+ ++FS F+ FLSLC GLRA   MA+D A+ TVKVG+VLDSDSSIGKMG+S MEMALS
Sbjct: 6   METPTVFSIFVFFLSLCIGLRAPPAMAEDAAIATVKVGVVLDSDSSIGKMGLSLMEMALS 65

Query: 61  DFYESHRNYKTRLALFAKNSMEDVIEATAAAIELINKEEVEAIVGPQNSMQAAFMADLGK 120
           DFY  HRNY+TRLALF KNSM DV+ A AAA++LI KEEVEAIVGPQ+S QA FMADLGK
Sbjct: 66  DFYGFHRNYRTRLALFPKNSMGDVVGAAAAALDLIKKEEVEAIVGPQSSTQATFMADLGK 125

Query: 121 KSQVPIISFSVTTPSLNPHRNPYFFRASQIDSSQAKPIAAIFEAFDWGQGVLIHSDDEYG 180
           KSQVPIIS S TTP LNP RNPYFFRA+QIDSSQ +PIAAI +AF+W Q V+IHSDD+YG
Sbjct: 126 KSQVPIISISATTPPLNPRRNPYFFRATQIDSSQVQPIAAIIKAFNWRQAVIIHSDDQYG 185

Query: 181 EGFLPSMRDALGETNIRVAYETAIPQSASDDRIAKELYKLMTMQTRVFVVHMLPELGSRL 240
           EG LPSMRDAL E N R+ YETAIPQSASDDRIAKELYKLMTMQTRVFVVHMLPELG+R+
Sbjct: 186 EGLLPSMRDALREINTRI-YETAIPQSASDDRIAKELYKLMTMQTRVFVVHMLPELGTRV 245

Query: 241 FAMAKEIGMMGSGYVWIITDGMSNFLSRIDDSSMVAMSGALGVRTYIPRTEKLEAFQMRW 300
           F+MAKEIGMMG+GYVWIITDGMSN  S IDDS++ AMSGALGVRT+IPRT +LE FQ++W
Sbjct: 246 FSMAKEIGMMGAGYVWIITDGMSNSPSHIDDSAVEAMSGALGVRTHIPRTRELEDFQLKW 305

Query: 301 RRKFEKEISELNIFGLRAYDAIFALAKAVESVGTTEFIFEKSNVSSKFTDLDNLGVSRNG 360
            R+FE+EISELNIFGLRAYDA FALAKAVE VGTTEFIF+KS+VS K TDLDNLGVS+NG
Sbjct: 306 GRRFEQEISELNIFGLRAYDATFALAKAVEKVGTTEFIFKKSSVSGKSTDLDNLGVSKNG 365

Query: 361 PRLSEALSKTHFKGLAGDFRMVEMKLKSSTYEIINIRHEKNITVVGYWTPENGLTQTLDF 420
           PRLSEALSKTHFKGLAGDFRMVE +L+SSTYEIIN  + K ITVVG+WTPENGLTQTL+ 
Sbjct: 366 PRLSEALSKTHFKGLAGDFRMVEGQLQSSTYEIINF-NSKFITVVGFWTPENGLTQTLNS 425

Query: 421 MKTNSNTSVTNLSRIIWPGDDPNSFSFPKGWENPTNEKKLRIGIPVKSGVSKFVRARRDP 480
              +SNTSV NLS IIWPGD PNSFSFPKGWE PTNEKKLRIG+PVKSGVSKFVR   DP
Sbjct: 426 TNISSNTSVANLSHIIWPGDGPNSFSFPKGWETPTNEKKLRIGVPVKSGVSKFVREITDP 485

Query: 481 VTGWTKRTGYSIDIFEAVIKTLPYAVLYEYVPFANATGAMAGSYDDLVNQVYSGVYDAVV 540
           VT  TK TGYSIDIFEAVIK LPYAV YEY+ FA+  GAMAGSY D+V QV  GVYDAVV
Sbjct: 486 VTNRTKTTGYSIDIFEAVIKALPYAVSYEYIRFADKKGAMAGSYGDMVMQVRLGVYDAVV 545

Query: 541 GDVSIRESRSLHVDFTFPYSESSVSMVVLFRDIKNKKAWLFLKPLTLDLWLTSAFFFAFI 600
           GDVSIRESRS +VDFT PYSESSVSMVVLF+D  NKKAWLFLKPLTL+LW+T+AF FAFI
Sbjct: 546 GDVSIRESRSENVDFTLPYSESSVSMVVLFKD-NNKKAWLFLKPLTLNLWVTTAFSFAFI 605

Query: 601 GLVVWILEHRINEDFRGPPSHEIGTSFWFSFSTMVYAQREKVESNLARFVVIVWLFVVFI 660
           GLV+W+LEHRINEDFRGPPSHEIGTSFWF+FST+VYAQ+EKVESNLARFVVIVWLFVV I
Sbjct: 606 GLVIWVLEHRINEDFRGPPSHEIGTSFWFAFSTLVYAQKEKVESNLARFVVIVWLFVVLI 665

Query: 661 LTQGYTASLTSLLTVQKLEPTFTDMKQLKEQKLNVGYPHGSFVQALLIAQGFDPSKLVNY 720
           LTQ YTASLTSLLTVQKLEPTFTDM QLK+QK+NVGYP+GSFVQ LLIA+G D SKLV Y
Sbjct: 666 LTQSYTASLTSLLTVQKLEPTFTDMNQLKQQKVNVGYPNGSFVQELLIAEGLDRSKLVIY 725

Query: 721 NNMEHCGSLLLNRTIAAAFDEIPYLKVLTTTYCTNCT 756
           NNM HCG+LLLN TIAAAFDEIPYLKVLTTTYCTNCT
Sbjct: 726 NNMAHCGALLLNGTIAAAFDEIPYLKVLTTTYCTNCT 759

BLAST of Cucsat.G12823.T5 vs. ExPASy TrEMBL
Match: A0A6J1K8T1 (Glutamate receptor OS=Cucurbita maxima OX=3661 GN=LOC111492714 PE=3 SV=1)

HSP 1 Score: 1183 bits (3060), Expect = 0.0
Identity = 604/761 (79.37%), Postives = 667/761 (87.65%), Query Frame = 0

Query: 1   MESLSIFSTFIIFLSLCFGLRAFSVMADDEAVTVKVGLVLDSDSSIGKMGVSYMEMALSD 60
           ME  ++F  F+  L+LCFGLRAFS MADD+AV VKVG+VLDS S IGKMG+S+M++ALSD
Sbjct: 1   MEKSTVFFFFL--LALCFGLRAFSAMADDDAVKVKVGVVLDSSSLIGKMGLSFMDIALSD 60

Query: 61  FYESHRNYKTRLALFAKNSMEDVIEATAAAIELINKEEVEAIVGPQNSMQAAFMADLGKK 120
           FYE H+NYKTRL LF KNSM D +EATAAA+ELI KEEVEAIVGPQ+S QAAFMA LG+K
Sbjct: 61  FYEFHKNYKTRLTLFPKNSMGDDLEATAAALELIKKEEVEAIVGPQSSSQAAFMAVLGQK 120

Query: 121 SQVPIISFSVTTP--SLNPHRNPYFFRASQIDSSQAKPIAAIFEAFDWGQGVLIHSDDEY 180
           S VPIISFS TT   SL+  RNPYFFRA+QIDS Q KPIA+IF+ F+W Q VLIHSDD+Y
Sbjct: 121 SHVPIISFSATTTMQSLSSRRNPYFFRATQIDSLQVKPIASIFKTFNWRQAVLIHSDDQY 180

Query: 181 GEGFLPSMRDALGETNIRVAYETAIPQSASDDRIAKELYKLMTMQTRVFVVHMLPELGSR 240
           GEG LPSMRDAL E N RVA ETAIPQSASDDRIAK+LY+LMTMQTRVFVVHM PELGSR
Sbjct: 181 GEGILPSMRDALREVNTRVADETAIPQSASDDRIAKQLYRLMTMQTRVFVVHMSPELGSR 240

Query: 241 LFAMAKEIGMMGSGYVWIITDGMSNFLSRIDDSSMVAMSGALGVRTYIPRTEKLEAFQMR 300
           +FAMAKEIGMMGSGY+WIITDGMSNFLS+IDDS+M AMSGAL VRTYIP+TEKLE FQ R
Sbjct: 241 VFAMAKEIGMMGSGYIWIITDGMSNFLSQIDDSAMEAMSGALAVRTYIPKTEKLEDFQRR 300

Query: 301 WRRKFEKEISELNIFGLRAYDAIFALAKAVESVGTTEFIFEKSNV-SSKFTDLDNLGVSR 360
           WRR FEKEISELNIFGLRAYDA FALAKAVE VGTTEFI +KSNV S K TDLDNLGVSR
Sbjct: 301 WRRGFEKEISELNIFGLRAYDATFALAKAVERVGTTEFIIKKSNVVSGKSTDLDNLGVSR 360

Query: 361 NGPRLSEALSKTHFKGLAGDFRMVEMKLKSSTYEIINIRHEKNITVVGYWTPENGLTQTL 420
           NGPRLSEALSKTHFKG+AGDFRMVE +LKSS YEIINI H+KNIT VG+WTPENGLTQTL
Sbjct: 361 NGPRLSEALSKTHFKGIAGDFRMVEGQLKSSAYEIININHQKNITAVGFWTPENGLTQTL 420

Query: 421 DFMKTNSNTSVTNLSRIIWPGDDPNSFSFPKGWENPTNEKKLRIGIPVKSGVSKFVRARR 480
           +  K +SNT+  NLSRI+WPGD   S SFPKGW +P NEKKLRIGIPVKSGVSKF+R   
Sbjct: 421 NSTKMSSNTAAANLSRIVWPGD---SSSFPKGWVSPINEKKLRIGIPVKSGVSKFIREIT 480

Query: 481 DPVTGWTKRTGYSIDIFEAVIKTLPYAVLYEYVPFANATGAMAGSYDDLVNQVYSGVYDA 540
           DP++  TK+TGYSIDIFEAV++TLPYA+ YEY+ +AN  G MAGSYDDL+NQV+ G YDA
Sbjct: 481 DPLSSNTKKTGYSIDIFEAVVETLPYALNYEYIRYANDVGVMAGSYDDLINQVHRGEYDA 540

Query: 541 VVGDVSIRESRSLHVDFTFPYSESSVSMVVLFRDIKNKKAWLFLKPLTLDLWLTSAFFFA 600
           VVGD+SIRE+RSL+VDFT PYSE+SVSMVVL+RD  NKKAWLFLKPLTL+LWLTS FFF 
Sbjct: 541 VVGDISIRENRSLYVDFTLPYSEASVSMVVLYRD-NNKKAWLFLKPLTLELWLTSGFFFV 600

Query: 601 FIGLVVWILEHRINEDFRGPPSHEIGTSFWFSFSTMVYAQREKVESNLARFVVIVWLFVV 660
           FIGLV+W LEHRINEDFRGPPSHEIGTSFWF+FSTMVYAQREKVESNLARFVVIVWLFVV
Sbjct: 601 FIGLVIWTLEHRINEDFRGPPSHEIGTSFWFAFSTMVYAQREKVESNLARFVVIVWLFVV 660

Query: 661 FILTQGYTASLTSLLTVQKLEPTFTDMKQLKEQKLNVGYPHGSFVQALLIAQGFDPSKLV 720
            ILTQ YTASLTSLLTVQKLEPTF DM +LKE+K+NVGYP GSFV  LLI +GFD SKLV
Sbjct: 661 LILTQSYTASLTSLLTVQKLEPTFNDMNRLKEKKVNVGYPKGSFVHELLITEGFDQSKLV 720

Query: 721 NYNNMEHCGSLLLNRTIAAAFDEIPYLKVLTTTYCTNCTIM 758
            YNNM HCGSL LN TIAAAFDE+PYLK LTTTYCTNCTI+
Sbjct: 721 IYNNMAHCGSLFLNGTIAAAFDEVPYLKDLTTTYCTNCTIV 755

BLAST of Cucsat.G12823.T5 vs. ExPASy TrEMBL
Match: A0A6J1GB16 (Glutamate receptor OS=Cucurbita moschata OX=3662 GN=LOC111452368 PE=3 SV=1)

HSP 1 Score: 1177 bits (3046), Expect = 0.0
Identity = 605/754 (80.24%), Postives = 661/754 (87.67%), Query Frame = 0

Query: 8   STFIIFLSLCFGLRAFSVMADDEAVTVKVGLVLDSDSSIGKMGVSYMEMALSDFYESHRN 67
           ST   FL+LCFGLRA S MADDEAV VKVG+VLDS+S IGKMG+S+M+MALSDFYE H+N
Sbjct: 4   STVFFFLALCFGLRALSAMADDEAVKVKVGVVLDSNSLIGKMGLSFMDMALSDFYEFHKN 63

Query: 68  YKTRLALFAKNSMEDVIEATAAAIELINKEEVEAIVGPQNSMQAAFMADLGKKSQVPIIS 127
           Y TRL LF KNSM D +EATAAA+ELI KEEVEAIVGPQ+S QAAFMADLGKKS VPIIS
Sbjct: 64  YNTRLTLFPKNSMGDDLEATAAALELIKKEEVEAIVGPQSSSQAAFMADLGKKSHVPIIS 123

Query: 128 FSVTTP--SLNPHRNPYFFRASQIDSSQAKPIAAIFEAFDWGQGVLIHSDDEYGEGFLPS 187
           FS TT   SL+  RNPYFFRA+QIDSSQ KPIA+IF+ F+W Q V+IHSDD+YGEG LPS
Sbjct: 124 FSATTTMQSLSSRRNPYFFRATQIDSSQVKPIASIFKEFNWRQVVIIHSDDQYGEGILPS 183

Query: 188 MRDALGETNIRVAYETAIPQSASDDRIAKELYKLMTMQTRVFVVHMLPELGSRLFAMAKE 247
           MRDAL E N RVA ETAIPQSASDDRIAK+LY+LMTMQTRVFVVHM PELGSR+FAMAKE
Sbjct: 184 MRDALREINTRVADETAIPQSASDDRIAKQLYRLMTMQTRVFVVHMSPELGSRVFAMAKE 243

Query: 248 IGMMGSGYVWIITDGMSNFLSRIDDSSMVAMSGALGVRTYIPRTEKLEAFQMRWRRKFEK 307
           IGMMGSGY+WIITDGMSNFLS IDDS+M AMSGAL VRTYIP TEKLE FQ RWRR  +K
Sbjct: 244 IGMMGSGYIWIITDGMSNFLSEIDDSAMEAMSGALAVRTYIPITEKLEEFQRRWRR--DK 303

Query: 308 EISELNIFGLRAYDAIFALAKAVESVGTTEFIFEKSNV-SSKFTDLDNLGVSRNGPRLSE 367
           EISELNIFGLRAYDAIFALAKAVE VGTTEFIF+ SNV S K TDLDNLGVSRNGPRLSE
Sbjct: 304 EISELNIFGLRAYDAIFALAKAVERVGTTEFIFKNSNVVSGKSTDLDNLGVSRNGPRLSE 363

Query: 368 ALSKTHFKGLAGDFRMVEMKLKSSTYEIINIRHEKNITVVGYWTPENGLTQTLDFMKTNS 427
           ALSKTHFKG+AGDFRMVE +LKSS YEIINI H+KNIT VG+WTPENGLTQTL+  + +S
Sbjct: 364 ALSKTHFKGIAGDFRMVEGQLKSSAYEIININHQKNITAVGFWTPENGLTQTLNSTEMSS 423

Query: 428 NTSVTNLSRIIWPGDDPNSFSFPKGWENPTNEKKLRIGIPVKSGVSKFVRARRDPVTGWT 487
           N+S  NLSRIIWPGD   S SFPKGW +P NEKKLRIGIPVKSGVSKF+R  RDP++  T
Sbjct: 424 NSSAANLSRIIWPGD---SSSFPKGWVSPINEKKLRIGIPVKSGVSKFIREIRDPLSSNT 483

Query: 488 KRTGYSIDIFEAVIKTLPYAVLYEYVPFANATGAMAGSYDDLVNQVYSGVYDAVVGDVSI 547
           K+TGYSIDIFEAV++TLPYA+ YEY+ +AN  G MAGSYDDL+ QV  G YDAVVGD+SI
Sbjct: 484 KKTGYSIDIFEAVVETLPYALNYEYIRYANDVGVMAGSYDDLIKQVPRGEYDAVVGDISI 543

Query: 548 RESRSLHVDFTFPYSESSVSMVVLFRDIKNKKAWLFLKPLTLDLWLTSAFFFAFIGLVVW 607
           RESRSL+VDFT PYSE+SVSMVVL++D  NKKAWLFLKPLTLDLWLTS FFF FIGLV+W
Sbjct: 544 RESRSLYVDFTLPYSEASVSMVVLYQD-NNKKAWLFLKPLTLDLWLTSGFFFVFIGLVIW 603

Query: 608 ILEHRINEDFRGPPSHEIGTSFWFSFSTMVYAQREKVESNLARFVVIVWLFVVFILTQGY 667
            LEHRINEDFRGPPSHEIGTSFWF+FSTMVYAQREKVESNLARFVVIVWLFVV ILTQ Y
Sbjct: 604 TLEHRINEDFRGPPSHEIGTSFWFAFSTMVYAQREKVESNLARFVVIVWLFVVLILTQSY 663

Query: 668 TASLTSLLTVQKLEPTFTDMKQLKEQKLNVGYPHGSFVQALLIAQGFDPSKLVNYNNMEH 727
           TASLTSLLTVQKLEP F DM QLKE+K+NVGYP+GSFV  LLI +GFD SKLV YNNM H
Sbjct: 664 TASLTSLLTVQKLEPKFNDMNQLKEKKVNVGYPNGSFVLDLLITEGFDRSKLVIYNNMAH 723

Query: 728 CGSLLLNRTIAAAFDEIPYLKVLTTTYCTNCTIM 758
           CGSL LN TIAAAFDE+PYLK LTTTYCTNCTI+
Sbjct: 724 CGSLFLNGTIAAAFDEVPYLKDLTTTYCTNCTIV 751