Cucsat.G12395.T8 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G12395.T8
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Ammonium transporter
Location	ctg1837: 1868633 .. 1872085 (+)
RNA-Seq Expression	Cucsat.G12395.T8
Synteny	Cucsat.G12395.T8

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAAACACGTGAGAACACGGAAAGAGGAAAATGACTCCGCGGAGGAAGGAGGATGAAGAATCGCTTCCCATTTAGCACACTTCGCTTCTGGAAGTTCATACTTCCATCAAATTCTTTTACTCAAAACCCCTTTTATTATCCGACTCCTCTGTACAAAAATTTCAACTCCTCACCCAATTCCGTCAAAATCCTTCAAATCTCACTATCAACCCATCAATTCCTAGAAACCCACTTGTCATCTCCTTATAATTTCCTTCCTCCATCAATATTCCTTCATAAAACCTTCATTCTATCTTCCAACCCCGCAACCACCGGGAAACATGGCCGCCGGATCCCCATCATGGCTCAACACCGGCGACAATTCCTGGCAAATTACAGCCTCCACTCTCGTAGGACTTCAGAGCATGCCTGGACTTGTGATTCTCTACGCTAGCATCGTCAAAAAGAAATGGGCTGTTAATTCAGCGTTTATGGCTCTTTACGCCTTCGCCGCCGTTCTAATTTGTTGGGTCGTCGTCGGTTACAGAATGGCCTTCGGCGACCAGTTGATTCCTTTGTGGGGCAAAGGAATTCCAGCTCTGTCCCAAAGTTATCTAATAGGGCAAGGCAATATCCCTGAGAGTGGCGTGAACGATGGAAGAGGAACACCCAGAATTCAACCCAATGTTCCCATGGCTTCACTTGTGTATTTTCAATTTACTTTTGCCGCGATTACTGTGATTTTACTTGGTGGGTCTGTTCTTGCAAGAATGAACATCAAAGCTTGGATGGCCTTTGTTCCTCTTTGGGTAATTTTCTCATACACCGTCGGCGCATACAGTGTTTGGGGCGGTGGGTTTCTGTTTCAGTGGGGAGTAATTGATTACTCCGGTGGGTATGTAATTCATCTTTCGTCTGGAATTGCTGGGTTAACAGCAGCTTATTGGGTAAGAACTCGATTTGGGGTTTAGAATTGGGGGTGGGGGGGGGGGGGGGGGGTTAATTTGAATTTGAATTTGAATTTGAATTGCTGGGTTTTGTTGTTTTTGTGTTTGGGATTATTAGGTTGGGCCGAGGATAAAAAGTGACAGAGAGAGGTTTCCGCCGAATAACGTGTTGCTTATGTTGGCCGGAGCAGGGCTGTTGTGGATGGGGTGGTCGGGTTTTAACGGGGGAGCACCGCACTCGGCGAACGTGGTGGCGTCGATAGCGGTGCTGAATACGAACGTGAGTGCCGCGACGAGCTTGTTGGTTTGGACCATTTTGGATGTGTTCTACTTTGGGAAGCCATCGGTGATTGGAGCCATTCAGGGTATGATGACTGGCTTGGCTTGCGTTACACCTGGCGCTGGTAATTACCATATCAGCCTTATTTTAAACCTTTAATTATTCAACTCATATATACATACATTTATAATTATGTTTGATTTATATTTCAAGTACAAAAATCAAGAGTTTCGTCTAATTAATATAATTTTGTGTTAAATAACATTGCCTAAGATGCTCCTGAAAATACATGAGTTTTAAGGTTTCGAATTTAGAGTAAATTTGTTGATTTTATAAACACTTAGATATGTTAGGAACTTAGAGATGAAAGATTATAGTTTTAAAAAGGCAATATAGAATTCAATGTGAGACATGTGAACACAATAAGTTGACATATAATATATAACTTTGATCAAATTATCATTCAAATCCTTCTACTCGAAATCCTTATACTAAAATAAACAAAAAAAAAAAAAAATACATATATACGAATTAAAAGAGGGTGTGATATTAGAATGTCATTTTCTTGATATCTACATTTCAATGAGATGTTGATTATAAATATGATTTATGAAACCACAATAATAATTAAGATTTGAAAGAAACAATTGAAAACAGGAGTCGTGCAGACGTGGGCGGCTATCATAATGGGAATCCTAGCAGGAAGCATTCCATGGCTATCCATGATGGTCCTTCATAAAAAATTGAGTTTCTTGCAAAAGGTAATTTCTTCAACCTTCCATCTTAATCCATTTCTATATATTCGCTTCTCAATTTTCAAACAAAGTTTAATTCAATTATCTTTAATTAATTTATTAAAACTTGCTGATAAAGGACAATGGGGTTTCTGGAAACTACTGAGAATAATTGTTTTTTTTTTTCCCAAAAGAAAATGACTTCAAAATTAAATATTTCCAAATTGCTGCTAATTTTTTAATCATATTTGTTGTTTGACAAATCATTTTCATAAGGGGGCCCCTCCTAGTCCTTGGGTTGTTGCCTTTGTACTATTTTTGTTGTTTGTGGATTTTTCTTCCAAACTTCACTTTCCGCCATCTTTGAATATTATTCCACCCCGATACGACGTGGTTTCGGATCGGTATGCATTGTAAAAAAAACCGCTCTTTAAAGCCTTGCCTTCATTTTTCATTTTGCTTTTATTCAGAATCAAATAGGATTTTCCCTAAGTTTGGAAGAACGTTGGAATCTTAGGCAGAAGCGAAATTTTCAATTCATGAAATAAGAAAAAACACATTGATTAAAGGGAAATGCTCTAAAATAGCAAAGTGGGAAAATAATTTTTTAATAAAGAAAATGGCAAATTGTAGGTTTGGAAACTACTATTGTTTTTTGAAAAGTTGGTTCAATTGAAATAGATGTGTGAAGTTGGTAATTAACTAACCCATCACAATTTTTTTTAAGAACCTTCCATTCCCATACTTTACTAGACCATTAATGACTATTAAGAATGATTAAAACTCACACGTGTATACAACAGGCTGATGACACGCTTGGCGTATTTCACACGCACGCAGTGGCAGGATTAATGGGCGGGCTTTTAACTGGGCTTTTAGCGGAGCCCACTCTTTGCGATCTCTACCTACCGATCATCGGCACGCGCGGCGCCTTTTACGGCGGAAGCGGCGGCGTTCAGTTCCTTAAACAACTGGCTGGATCCACCTTCATCATAGGCTGGAACATAGTCTCCACCACCGTAATACTCCTCTTTATTCGCCTCTTTATGCCCTTGAGAATGCCCGATGAGGAACTCATGATCGGCGACGACGCCGTCCACGGCGAGGAGGCTTATGCTCTTTGGGGCGACGGCGAGAAATTCGATCCAAGAAGGCACGGCAATGCGGCAGCCAATATGGAAGAAGGAGCCGGATCGCCTTATATAAATGGTGCTAGAGGAGTGATCATCGAATTGTAAAACGCATTTTAAATTATTCTTTTGGTTTCATTCAAATTTGTGTATGTCTTCATGTATTATAATAGTGAGTGTTTCAGAATTTTTGGGTGAGTATCTGTTAAGAGTCTTTAACCATCCTGGTAAAGAAAAGTGTCCTTTTGATCTCTTATTAAGAAACGAAACTGTTGATTTAATGTACAAAATACAGAGTAATCAGATAAGGAGTAAGACTGAGAGAGAACCAAAAAGAAAAAAAAAAAAAGAGTGGAAAAAGCTGAGTTGCTTTGTAATCAGAA

Coding sequence (CDS)

ATGGCCGCCGGATCCCCATCATGGCTCAACACCGGCGACAATTCCTGGCAAATTACAGCCTCCACTCTCGTAGGACTTCAGAGCATGCCTGGACTTGTGATTCTCTACGCTAGCATCGTCAAAAAGAAATGGGCTGTTAATTCAGCGTTTATGGCTCTTTACGCCTTCGCCGCCGTTCTAATTTGTTGGGTCGTCGTCGGTTACAGAATGGCCTTCGGCGACCAGTTGATTCCTTTGTGGGGCAAAGGAATTCCAGCTCTGTCCCAAAGTTATCTAATAGGGCAAGGCAATATCCCTGAGAGTGGCGTGAACGATGGAAGAGGAACACCCAGAATTCAACCCAATGTTCCCATGGCTTCACTTGTGTATTTTCAATTTACTTTTGCCGCGATTACTGTGATTTTACTTGGTGGGTCTGTTCTTGCAAGAATGAACATCAAAGCTTGGATGGCCTTTGTTCCTCTTTGGGTAATTTTCTCATACACCGTCGGCGCATACAGTGTTTGGGGCGGTGGGTTTCTGTTTCAGTGGGGAGTAATTGATTACTCCGGTGGGTATGTAATTCATCTTTCGTCTGGAATTGCTGGGTTAACAGCAGCTTATTGGGTTGGGCCGAGGATAAAAAGTGACAGAGAGAGGTTTCCGCCGAATAACGTGTTGCTTATGTTGGCCGGAGCAGGGCTGTTGTGGATGGGGTGGTCGGGTTTTAACGGGGGAGCACCGCACTCGGCGAACGTGGTGGCGTCGATAGCGGTGCTGAATACGAACGTGAGTGCCGCGACGAGCTTGTTGGTTTGGACCATTTTGGATGTGTTCTACTTTGGGAAGCCATCGGTGATTGGAGCCATTCAGGGTATGATGACTGGCTTGGCTTGCGTTACACCTGGCGCTGGTAATTACCATATCAGCCTTATTTTAAACCTTTAA

Protein sequence

MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGVNDGRGTPRIQPNVPMASLVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAGNYHISLILNL

Homology

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BLAST of Cucsat.G12395.T8 vs. ExPASy Swiss-Prot
Match: Q9M6N7 (Ammonium transporter 2 OS=Arabidopsis thaliana OX=3702 GN=AMT2 PE=1 SV=2)

HSP 1 Score: 478.8 bits (1231), Expect = 4.7e-134
Identity = 234/293 (79.86%), Postives = 258/293 (88.05%), Query Frame = 0


Query: 6   PSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLICWVV 65
           P WLN GDN+WQ+TA+TLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL+CWV+
Sbjct: 13  PEWLNKGDNAWQLTAATLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLLCWVL 72

Query: 66  VGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGVNDGRGTPRIQPNVPMASLVYFQ 125
           + Y+MAFG++L+P WGKG PA  Q YL GQ  IP S V          P  PMA+LVYFQ
Sbjct: 73  LCYKMAFGEELLPFWGKGGPAFDQGYLKGQAKIPNSNV--------AAPYFPMATLVYFQ 132

Query: 126 FTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVIDYSGG 185
           FTFAAIT IL+ GSVL RMNIKAWMAFVPLW+IFSYTVGAYS+WGGGFL+QWGVIDYSGG
Sbjct: 133 FTFAAITTILVAGSVLGRMNIKAWMAFVPLWLIFSYTVGAYSIWGGGFLYQWGVIDYSGG 192

Query: 186 YVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPHSAN 245
           YVIHLSSG+AG  AAYWVGPR K+DRERFPPNNVLLMLAGAGLLWMGWSGFNGGAP++AN
Sbjct: 193 YVIHLSSGVAGFVAAYWVGPRPKADRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPYAAN 252

Query: 246 VVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG 299
           + +SIAVLNTN+SAATSLLVWT LDV +FGKPSVIGAIQGM+TGLA VTPGAG
Sbjct: 253 LTSSIAVLNTNLSAATSLLVWTTLDVIFFGKPSVIGAIQGMVTGLAGVTPGAG 297

[Close]

BLAST of Cucsat.G12395.T8 vs. ExPASy Swiss-Prot
Match: Q69T29 (Ammonium transporter 3 member 3 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT3-3 PE=2 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 1.7e-128
Identity = 217/297 (73.06%), Postives = 257/297 (86.53%), Query Frame = 0


Query: 5   SPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLICWV 64
           SP WLN GDN+WQ+T++TLVGLQSMPGLVILY SIVKKKWA+NSAFMALYAFAAV ICWV
Sbjct: 16  SPDWLNKGDNAWQMTSATLVGLQSMPGLVILYGSIVKKKWAINSAFMALYAFAAVWICWV 75

Query: 65  VVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGV---NDGRGTPRIQPNVPMASL 124
           V  Y M+FGD+L+P WGK  PAL QS+L+ Q  +  + +   N     P ++P  P+A++
Sbjct: 76  VWAYNMSFGDRLLPFWGKARPALGQSFLVAQSELTATAIRYHNGSAEAPMLKPLYPVATM 135

Query: 125 VYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVID 184
           VYFQ  FA+IT+I+L GS+L RMNIKAWMAFVPLW+ FSYTV A+S+WGGGFLFQWGVID
Sbjct: 136 VYFQCMFASITIIILAGSLLGRMNIKAWMAFVPLWITFSYTVCAFSLWGGGFLFQWGVID 195

Query: 185 YSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAP 244
           YSGGYVIHLSSGIAGLTAAYWVGPR  SDRERFPPNN+LL+LAGAGLLW+GW+GFNGG P
Sbjct: 196 YSGGYVIHLSSGIAGLTAAYWVGPRSASDRERFPPNNILLVLAGAGLLWLGWTGFNGGDP 255

Query: 245 HSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG 299
           +SAN+ +S+AVLNT++ A+TSLLVWTILDVF+FGKPSVIGA+QGM+TGL C+TPGAG
Sbjct: 256 YSANIDSSMAVLNTHICASTSLLVWTILDVFFFGKPSVIGAVQGMITGLVCITPGAG 312

[Close]

BLAST of Cucsat.G12395.T8 vs. ExPASy Swiss-Prot
Match: Q84KJ7 (Ammonium transporter 2 member 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT2-1 PE=2 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 2.2e-128
Identity = 217/293 (74.06%), Postives = 254/293 (86.69%), Query Frame = 0


Query: 6   PSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLICWVV 65
           P WLN GDN+WQ+TASTLVG+QSMPGLV+LY SIVKKKWAVNSAFMALYA+A+ L+ WV+
Sbjct: 15  PDWLNKGDNAWQLTASTLVGIQSMPGLVVLYGSIVKKKWAVNSAFMALYAYASSLLVWVL 74

Query: 66  VGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGVNDGRGTPRIQPNVPMASLVYFQ 125
           VG+RMAFGDQL+P WGK   AL+QSYL+G+  +P +        PR +P  P A+LV FQ
Sbjct: 75  VGFRMAFGDQLLPFWGKAGVALTQSYLVGRATLPATAHG---AIPRTEPFYPEATLVLFQ 134

Query: 126 FTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVIDYSGG 185
           F FAAIT++LL GSVL RMNIKAWMAF PLW++ SYTVGA+S+WGGGFL++WGVIDYSGG
Sbjct: 135 FEFAAITLVLLAGSVLGRMNIKAWMAFTPLWLLLSYTVGAFSLWGGGFLYRWGVIDYSGG 194

Query: 186 YVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPHSAN 245
           YVIHLSSGIAG TAAYWVGPR+KSDRERF PNN+LLM+AG GLLWMGW+GFNGGAP++AN
Sbjct: 195 YVIHLSSGIAGFTAAYWVGPRLKSDRERFSPNNILLMIAGGGLLWMGWAGFNGGAPYAAN 254

Query: 246 VVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG 299
           + AS+AVLNTNV AATSLL+WT LDV +F KPSVIGA+QGMMTGL C+TPGAG
Sbjct: 255 IAASVAVLNTNVCAATSLLMWTCLDVIFFRKPSVIGAVQGMMTGLVCITPGAG 304

[Close]

BLAST of Cucsat.G12395.T8 vs. ExPASy Swiss-Prot
Match: Q8S233 (Ammonium transporter 2 member 3 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT2-3 PE=2 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 1.2e-126
Identity = 213/299 (71.24%), Postives = 253/299 (84.62%), Query Frame = 0


Query: 6   PSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLICWVV 65
           P WLNTGDN WQ+ A+T VGLQSMPGLV+LY SIVKKKWAVNSAFMALYA+A+ LI WV+
Sbjct: 18  PEWLNTGDNGWQLAAATFVGLQSMPGLVVLYGSIVKKKWAVNSAFMALYAYASTLIVWVL 77

Query: 66  VGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPES---GVNDGRGTPRIQPNVPMASLV 125
           VG+RMAFGD+L+P WGK   AL++ +L+ + ++P +   G +    +PR +P  P AS+V
Sbjct: 78  VGFRMAFGDRLLPFWGKAGAALTEGFLVARASVPATAHYGKDGALESPRTEPFYPEASMV 137

Query: 126 YFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVIDY 185
            FQF  AAIT++LL GS+L RMNIKAWMAF PLW++FSYTV A+S+WGGGFL+QWGVIDY
Sbjct: 138 LFQFELAAITLVLLAGSLLGRMNIKAWMAFTPLWLLFSYTVCAFSLWGGGFLYQWGVIDY 197

Query: 186 SGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPH 245
           SGGYVIHLSSGIAG TAAYWVGPR+KSDRERF PNN+LLM+AG GLLW+GW+GFNGGAP+
Sbjct: 198 SGGYVIHLSSGIAGFTAAYWVGPRLKSDRERFSPNNILLMIAGGGLLWLGWAGFNGGAPY 257

Query: 246 SANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAGNYH 302
           + N+ ASIAVLNTNVSAA SLL WT LDV +FGKPSVIGA+QGMMTGL C+TPGAG  H
Sbjct: 258 APNITASIAVLNTNVSAAASLLTWTCLDVIFFGKPSVIGAVQGMMTGLVCITPGAGLVH 316

[Close]

BLAST of Cucsat.G12395.T8 vs. ExPASy Swiss-Prot
Match: Q8S230 (Ammonium transporter 2 member 2 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT2-2 PE=2 SV=2)

HSP 1 Score: 449.5 bits (1155), Expect = 3.0e-125
Identity = 210/299 (70.23%), Postives = 253/299 (84.62%), Query Frame = 0


Query: 6   PSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLICWVV 65
           P+WLN GD +WQ+ A+T VG+QSMPGLV++Y SIVKKKWAVNSAFMALYA+A+ LI WV+
Sbjct: 22  PAWLNKGDTAWQLVAATFVGIQSMPGLVVIYGSIVKKKWAVNSAFMALYAYASTLIVWVL 81

Query: 66  VGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPES---GVNDGRGTPRIQPNVPMASLV 125
           VG+RMAFGD+L+P W K  PAL+Q +L+ +   P +   G +    TPR +P    A+LV
Sbjct: 82  VGFRMAFGDRLLPFWAKAGPALTQDFLVQRAVFPATAHYGSDGTLETPRTEPFYAEAALV 141

Query: 126 YFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVIDY 185
            F+F FAAIT++LL GS+L RMNIKAWMAF PLW++FSYTVGA+S+WGGGFL+QWGVIDY
Sbjct: 142 LFEFEFAAITLVLLAGSLLGRMNIKAWMAFTPLWLLFSYTVGAFSLWGGGFLYQWGVIDY 201

Query: 186 SGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPH 245
           SGGYVIHLSSG+AG TAAYWVGPR+KSDRERF PNN+LLM+AG GLLW+GW+GFNGGAP+
Sbjct: 202 SGGYVIHLSSGVAGFTAAYWVGPRLKSDRERFSPNNILLMIAGGGLLWLGWAGFNGGAPY 261

Query: 246 SANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAGNYH 302
           + NV A++AVLNTNVSAATSLL WT LDV +FGKPSVIGA+QGMMTGL C+TPGAG  H
Sbjct: 262 APNVTATVAVLNTNVSAATSLLTWTCLDVIFFGKPSVIGAVQGMMTGLVCITPGAGLVH 320

[Close]

BLAST of Cucsat.G12395.T8 vs. NCBI nr
Match: XP_004137698.1 (ammonium transporter 2 [Cucumis sativus] >KGN58729.1 hypothetical protein Csa_000983 [Cucumis sativus])

HSP 1 Score: 597 bits (1540), Expect = 2.19e-212
Identity = 298/298 (100.00%), Postives = 298/298 (100.00%), Query Frame = 0


Query: 1   MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60
           MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL
Sbjct: 1   MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60

Query: 61  ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGVNDGRGTPRIQPNVPMAS 120
           ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGVNDGRGTPRIQPNVPMAS
Sbjct: 61  ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGVNDGRGTPRIQPNVPMAS 120

Query: 121 LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI 180
           LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI
Sbjct: 121 LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI 180

Query: 181 DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA 240
           DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA
Sbjct: 181 DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA 240

Query: 241 PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG 298
           PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG
Sbjct: 241 PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG 298

[Close]

BLAST of Cucsat.G12395.T8 vs. NCBI nr
Match: XP_008442385.1 (PREDICTED: ammonium transporter 2 [Cucumis melo] >KAA0044194.1 ammonium transporter 2 [Cucumis melo var. makuwa] >TYK24938.1 ammonium transporter 2 [Cucumis melo var. makuwa])

HSP 1 Score: 586 bits (1511), Expect = 5.66e-208
Identity = 291/298 (97.65%), Postives = 293/298 (98.32%), Query Frame = 0


Query: 1   MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60
           MAA SPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL
Sbjct: 1   MAAASPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60

Query: 61  ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGVNDGRGTPRIQPNVPMAS 120
           ICWV+VGYRMAFGDQLIP WGKGIPALSQ+YLI QGNIPESG NDG GTPRIQPNVPMAS
Sbjct: 61  ICWVIVGYRMAFGDQLIPFWGKGIPALSQNYLIAQGNIPESGANDGNGTPRIQPNVPMAS 120

Query: 121 LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI 180
           LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI
Sbjct: 121 LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI 180

Query: 181 DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA 240
           DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA
Sbjct: 181 DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA 240

Query: 241 PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG 298
           PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG
Sbjct: 241 PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG 298

[Close]

BLAST of Cucsat.G12395.T8 vs. NCBI nr
Match: XP_038904152.1 (ammonium transporter 2 [Benincasa hispida])

HSP 1 Score: 581 bits (1497), Expect = 1.05e-205
Identity = 288/298 (96.64%), Postives = 292/298 (97.99%), Query Frame = 0


Query: 1   MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60
           MAA SP WLNTGDNSWQITASTLVGLQSMPGLVILY SIVKKKWAVNSAFMALYAFAAVL
Sbjct: 10  MAAASPPWLNTGDNSWQITASTLVGLQSMPGLVILYGSIVKKKWAVNSAFMALYAFAAVL 69

Query: 61  ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGVNDGRGTPRIQPNVPMAS 120
           ICWV+VGYRMAFGDQLIPLWGKGIPALSQ YLI Q NIPESG+NDG+GTPRIQPNVPMAS
Sbjct: 70  ICWVIVGYRMAFGDQLIPLWGKGIPALSQDYLINQANIPESGLNDGQGTPRIQPNVPMAS 129

Query: 121 LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI 180
           LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLF+WGVI
Sbjct: 130 LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFKWGVI 189

Query: 181 DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA 240
           DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA
Sbjct: 190 DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA 249

Query: 241 PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG 298
           PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG
Sbjct: 250 PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG 307

[Close]

BLAST of Cucsat.G12395.T8 vs. NCBI nr
Match: XP_022934329.1 (ammonium transporter 2-like [Cucurbita moschata] >KAG6580457.1 Ammonium transporter 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 562 bits (1449), Expect = 2.25e-198
Identity = 282/300 (94.00%), Postives = 289/300 (96.33%), Query Frame = 0


Query: 1   MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60
           MAAGSP WLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM LYAFAAVL
Sbjct: 10  MAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMVLYAFAAVL 69

Query: 61  ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGV--NDGRGTPRIQPNVPM 120
           ICWV+VGYRMAFGDQL+PLWGKGIPAL+QSYLI + NIPESG   NDGR T RI+PN+PM
Sbjct: 70  ICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVTLRIEPNIPM 129

Query: 121 ASLVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWG 180
           ASLVYFQFTFAAITVILL GSVLARMNIKAWMAFVPLWVIF+YTVGAYSVWGGGFLFQWG
Sbjct: 130 ASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVWGGGFLFQWG 189

Query: 181 VIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNG 240
           VIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNG
Sbjct: 190 VIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNG 249

Query: 241 GAPHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG 298
           GAPHSANVVASIAVLNTNVSAATSLLVWTILDV YFGKPSVIGAIQGMMTGLACVTPGAG
Sbjct: 250 GAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTGLACVTPGAG 309

[Close]

BLAST of Cucsat.G12395.T8 vs. NCBI nr
Match: XP_023529182.1 (ammonium transporter 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 562 bits (1449), Expect = 2.25e-198
Identity = 282/300 (94.00%), Postives = 289/300 (96.33%), Query Frame = 0


Query: 1   MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60
           MAAGSP WLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM LYAFAAVL
Sbjct: 10  MAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMVLYAFAAVL 69

Query: 61  ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGV--NDGRGTPRIQPNVPM 120
           ICWV+VGYRMAFGDQL+PLWGKGIPAL+QSYLI + NIPESG   NDGR T RI+PN+PM
Sbjct: 70  ICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVTLRIEPNIPM 129

Query: 121 ASLVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWG 180
           ASLVYFQFTFAAITVILL GSVLARMNIKAWMAFVPLWVIF+YTVGAYSVWGGGFLFQWG
Sbjct: 130 ASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVWGGGFLFQWG 189

Query: 181 VIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNG 240
           VIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNG
Sbjct: 190 VIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNG 249

Query: 241 GAPHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG 298
           GAPHSANVVASIAVLNTNVSAATSLLVWTILDV YFGKPSVIGAIQGMMTGLACVTPGAG
Sbjct: 250 GAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTGLACVTPGAG 309

[Close]

BLAST of Cucsat.G12395.T8 vs. ExPASy TrEMBL
Match: A0A0A0LCQ1 (Ammonium transporter OS=Cucumis sativus OX=3659 GN=Csa_3G730930 PE=3 SV=1)

HSP 1 Score: 597 bits (1540), Expect = 1.06e-212
Identity = 298/298 (100.00%), Postives = 298/298 (100.00%), Query Frame = 0


Query: 1   MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60
           MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL
Sbjct: 1   MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60

Query: 61  ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGVNDGRGTPRIQPNVPMAS 120
           ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGVNDGRGTPRIQPNVPMAS
Sbjct: 61  ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGVNDGRGTPRIQPNVPMAS 120

Query: 121 LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI 180
           LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI
Sbjct: 121 LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI 180

Query: 181 DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA 240
           DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA
Sbjct: 181 DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA 240

Query: 241 PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG 298
           PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG
Sbjct: 241 PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG 298

[Close]

BLAST of Cucsat.G12395.T8 vs. ExPASy TrEMBL
Match: A0A5A7TLX4 (Ammonium transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00540 PE=3 SV=1)

HSP 1 Score: 586 bits (1511), Expect = 2.74e-208
Identity = 291/298 (97.65%), Postives = 293/298 (98.32%), Query Frame = 0


Query: 1   MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60
           MAA SPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL
Sbjct: 1   MAAASPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60

Query: 61  ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGVNDGRGTPRIQPNVPMAS 120
           ICWV+VGYRMAFGDQLIP WGKGIPALSQ+YLI QGNIPESG NDG GTPRIQPNVPMAS
Sbjct: 61  ICWVIVGYRMAFGDQLIPFWGKGIPALSQNYLIAQGNIPESGANDGNGTPRIQPNVPMAS 120

Query: 121 LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI 180
           LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI
Sbjct: 121 LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI 180

Query: 181 DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA 240
           DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA
Sbjct: 181 DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA 240

Query: 241 PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG 298
           PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG
Sbjct: 241 PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG 298

[Close]

BLAST of Cucsat.G12395.T8 vs. ExPASy TrEMBL
Match: A0A1S3B5K4 (Ammonium transporter OS=Cucumis melo OX=3656 GN=LOC103486266 PE=3 SV=1)

HSP 1 Score: 586 bits (1511), Expect = 2.74e-208
Identity = 291/298 (97.65%), Postives = 293/298 (98.32%), Query Frame = 0


Query: 1   MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60
           MAA SPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL
Sbjct: 1   MAAASPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60

Query: 61  ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGVNDGRGTPRIQPNVPMAS 120
           ICWV+VGYRMAFGDQLIP WGKGIPALSQ+YLI QGNIPESG NDG GTPRIQPNVPMAS
Sbjct: 61  ICWVIVGYRMAFGDQLIPFWGKGIPALSQNYLIAQGNIPESGANDGNGTPRIQPNVPMAS 120

Query: 121 LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI 180
           LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI
Sbjct: 121 LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI 180

Query: 181 DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA 240
           DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA
Sbjct: 181 DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA 240

Query: 241 PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG 298
           PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG
Sbjct: 241 PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG 298

[Close]

BLAST of Cucsat.G12395.T8 vs. ExPASy TrEMBL
Match: A0A6J1F297 (Ammonium transporter OS=Cucurbita moschata OX=3662 GN=LOC111441525 PE=3 SV=1)

HSP 1 Score: 562 bits (1449), Expect = 1.09e-198
Identity = 282/300 (94.00%), Postives = 289/300 (96.33%), Query Frame = 0


Query: 1   MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60
           MAAGSP WLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM LYAFAAVL
Sbjct: 10  MAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMVLYAFAAVL 69

Query: 61  ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGV--NDGRGTPRIQPNVPM 120
           ICWV+VGYRMAFGDQL+PLWGKGIPAL+QSYLI + NIPESG   NDGR T RI+PN+PM
Sbjct: 70  ICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVTLRIEPNIPM 129

Query: 121 ASLVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWG 180
           ASLVYFQFTFAAITVILL GSVLARMNIKAWMAFVPLWVIF+YTVGAYSVWGGGFLFQWG
Sbjct: 130 ASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVWGGGFLFQWG 189

Query: 181 VIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNG 240
           VIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNG
Sbjct: 190 VIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNG 249

Query: 241 GAPHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG 298
           GAPHSANVVASIAVLNTNVSAATSLLVWTILDV YFGKPSVIGAIQGMMTGLACVTPGAG
Sbjct: 250 GAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTGLACVTPGAG 309

[Close]

BLAST of Cucsat.G12395.T8 vs. ExPASy TrEMBL
Match: A0A6J1IZU0 (Ammonium transporter OS=Cucurbita maxima OX=3661 GN=LOC111481439 PE=3 SV=1)

HSP 1 Score: 560 bits (1444), Expect = 6.28e-198
Identity = 281/300 (93.67%), Postives = 289/300 (96.33%), Query Frame = 0


Query: 1   MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60
           MAAGSP WLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM LYAFAAVL
Sbjct: 10  MAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMVLYAFAAVL 69

Query: 61  ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGV--NDGRGTPRIQPNVPM 120
           ICWV+VGYRMAFGDQL+PLWGKGIPAL+QSYLI + +IPESG   NDGR T RI+PN+PM
Sbjct: 70  ICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRADIPESGNTNNDGRVTLRIEPNIPM 129

Query: 121 ASLVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWG 180
           ASLVYFQFTFAAITVILL GSVLARMNIKAWMAFVPLWVIF+YTVGAYSVWGGGFLFQWG
Sbjct: 130 ASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVWGGGFLFQWG 189

Query: 181 VIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNG 240
           VIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNG
Sbjct: 190 VIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNG 249

Query: 241 GAPHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG 298
           GAPHSANVVASIAVLNTNVSAATSLLVWTILDV YFGKPSVIGAIQGMMTGLACVTPGAG
Sbjct: 250 GAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTGLACVTPGAG 309

The following BLAST results are available for this feature:

BLAST of Cucsat.G12395.T8 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M6N7	4.7e-134	79.86	Ammonium transporter 2 OS=Arabidopsis thaliana OX=3702 GN=AMT2 PE=1 SV=2	[more]
Q69T29	1.7e-128	73.06	Ammonium transporter 3 member 3 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT3...	[more]
Q84KJ7	2.2e-128	74.06	Ammonium transporter 2 member 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT2...	[more]
Q8S233	1.2e-126	71.24	Ammonium transporter 2 member 3 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT2...	[more]
Q8S230	3.0e-125	70.23	Ammonium transporter 2 member 2 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT2...	[more]

BLAST of Cucsat.G12395.T8 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004137698.1	2.19e-212	100.00	ammonium transporter 2 [Cucumis sativus] >KGN58729.1 hypothetical protein Csa_00...	[more]
XP_008442385.1	5.66e-208	97.65	PREDICTED: ammonium transporter 2 [Cucumis melo] >KAA0044194.1 ammonium transpor...	[more]
XP_038904152.1	1.05e-205	96.64	ammonium transporter 2 [Benincasa hispida]	[more]
XP_022934329.1	2.25e-198	94.00	ammonium transporter 2-like [Cucurbita moschata] >KAG6580457.1 Ammonium transpor...	[more]
XP_023529182.1	2.25e-198	94.00	ammonium transporter 2-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cucsat.G12395.T8 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LCQ1	1.06e-212	100.00	Ammonium transporter OS=Cucumis sativus OX=3659 GN=Csa_3G730930 PE=3 SV=1	[more]
A0A5A7TLX4	2.74e-208	97.65	Ammonium transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352...	[more]
A0A1S3B5K4	2.74e-208	97.65	Ammonium transporter OS=Cucumis melo OX=3656 GN=LOC103486266 PE=3 SV=1	[more]
A0A6J1F297	1.09e-198	94.00	Ammonium transporter OS=Cucurbita moschata OX=3662 GN=LOC111441525 PE=3 SV=1	[more]
A0A6J1IZU0	6.28e-198	93.67	Ammonium transporter OS=Cucurbita maxima OX=3661 GN=LOC111481439 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002229	Blood group Rhesus C/E/D polypeptide	PRINTS	PR00342	RHESUSRHD	coord: 125..141 score: 31.7 coord: 251..273 score: 24.88 coord: 220..238 score: 41.52
IPR029020	Ammonium/urea transporter	GENE3D	1.10.3430.10	Ammonium transporter AmtB like domains	coord: 1..306 e-value: 2.2E-80 score: 272.5
IPR024041	Ammonium transporter AmtB-like domain	PFAM	PF00909	Ammonium_transp	coord: 16..300 e-value: 4.8E-52 score: 177.0
None	No IPR available	PANTHER	PTHR43029:SF34	AMMONIUM TRANSPORTER 2	coord: 3..299
None	No IPR available	PANTHER	PTHR43029	AMMONIUM TRANSPORTER MEP2	coord: 3..299
None	No IPR available	SUPERFAMILY	111352	Ammonium transporter	coord: 8..302
IPR018047	Ammonium transporter, conserved site	PROSITE	PS01219	AMMONIUM_TRANSP	coord: 181..206

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G12395	Cucsat.G12395	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G12395.T8.E1	Cucsat.G12395.T8.E1	exon
Cucsat.G12395.T8.E2	Cucsat.G12395.T8.E2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G12395.T8.C1	Cucsat.G12395.T8.C1	CDS
Cucsat.G12395.T8.C2	Cucsat.G12395.T8.C2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G12395.T8	Cucsat.G12395.T8-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0072488	ammonium transmembrane transport
biological_process	GO:0015696	ammonium transport
biological_process	GO:0019740	nitrogen utilization
cellular_component	GO:0005887	integral component of plasma membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0008519	ammonium transmembrane transporter activity
molecular_function	GO:0015398	high-affinity secondary active ammonium transmembrane transporter activity