Cucsat.G12140.T2 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G12140.T2
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionCytochrome P450
Locationctg1837: 979199 .. 986135 (-)
RNA-Seq ExpressionCucsat.G12140.T2
SyntenyCucsat.G12140.T2
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CATCTCCTTCTCAATTCAATCATATAAATATATAACCCCATCAAATTCACTTCACTCCCTTCTTCCTTCCACACACACCTAACAATAATGCCGGACGCCGCTCTTCACTACACTCTCTACCTTCTCCTCTTCTTCCTCTCCTCTCTCCTTCTCTTCTTCTTCCTCCACAAACCCTCCAAATCCGCCTCCGCAAATCTCACTCTACCTCCATCTCCACCGCCGCTCCCAGTCATCGGCCACCTCCATCTCCTCCTCCCTGCCACTCACAAATCCTTCTTCAACATCTCCTCTAAGTACGGTCCGCTCCTCCACATCCGCCTCGGCGCTCTGCAGTACGTCCTCGTCTCCTCCGCCTCCCTCGCTGCTGAGATCTTCAAAACGCACGATCTCACCTTCTCCTCCCGACCTGATTTCGCCTTCAGCGAGGAGTATCCGTACGGAAAAGTGGGATTCCTTGGCGCTCCGTACGGGGATTATTGGCGGTATATGAAGAAGCTGACGATGATGGAGGTTCTTGCGGCGCCACAGCTTGCACGATCGCGATTTGTGAGGAATGAAGAGATCCTTCGTATGCTTCAAAAGTTGCTGCTCTGTTCCAAGCAGAAGCAGGTACTCTCTTAACTAACTAACTCCTTTTCTGTTTTCTTCACTATTTCTTTATTAGATTTTTTTTGCAAGCTATGGTTTTTTCTTTTATTCTTAATTTCTATTAATTACTAATTAATTAAAAAATTAAAATTAATTATGATATAAAAGTTTAGGTCACATTTTTACCACGATTTTAGACTCAATAATCAATTATTGATATACTTCTATTATTGTCTTTTATTAGGAATAATTTTTAAAATTTTACTGTATTTTATAAATATTTTTTTAAAAAATATAATGCCACACCCCACCCCGCCTCGAGCACCTACGTATTCAACCCGAAATGTGGCATGAAGCCAACCGTTCTTAACAACATCAATTGATCCTAACTACTATTCTTTTACAATGCTAAACAACTTAGAAAAAATTAAAACTTATGCAACAGAAACTACGAGAAACAACTTGAACAACTTCTTATACTTTAAACAGGTAGTCGAGGCTAAATGTCATTGATTACAAACTTTACTTACATATCAAACATAAATTATACAACAACACTTAACCTTAAGCAAAACCATGTACACGATAAGATTAAAACTGATCTTTAATATATAAACTATCAACAACTTCGCTCAGGAGTCTTGAAAACAACGGTTAGGTTCTCGGATCACGACCTCTACCTGAAAAGTGGAAAAATATTTTAAAAGGATGAGCTAAAAAGCCTAGTAAGTGATGGTTTTATAAAATAGACTTTTAAAACAAACATACTTTAAACATTTCATAAACATACCATATAATTTTAAGAAATCTCTTTAAACTGAACACTTTCTAACATTCGAATATTCATTTAACTTTGTATACTCTATACTTAGAAAAACTTAAACAAGATAGCAGAGCACCTTCGATCATACTATCCAATTAAACCTCAATAGAATAGCAAAGCACGTTCGGTCATACTATTCATAATAAACTCGATCAAGATAGTAGAACACATTTGGTAATATTATTCTTGCATGAACTCGATGGTAGTGACTTAGTGGTCAGCATAGCCCTATATCCCTGACTATGCACATTGACCATCAAGAACTCGGTATATAAAGTACCATGGTTTTCTCTTTAAAACCATTATATTAAACTCATGTTTTTCATAAATCATTGCTTGTAAATCTTATAAATACATGCTTGCTAAAAACATTTATAAACTCATGCTTAGAACACTTTTTTAAATAATACGCTTTAACACTTTTTCATAAACATGTTGATAAACAAAAATAAGGCTCGCTTAAAAAGCATACTTAAATAATAACTTGTAAAACATATTAGAATTATTCTTTAGGAATCATTTTACTACTCATATATCATAAGTTGAAATAACTTTAGTAAACTCATTTTTCACTCACAGATCGCAGAACAAACCCTTCGTCGATATGCTAGTCCAAATATCTCTTGTCCTGAAATAATGGAAAAGAACTTCAATTAATACCCTTTTATTTTAAAACATCAAACTTACTTAAAGTTTCCTAAATCATAAAAACAACCTCAAAACGTCTCGAAAAACCTCTCTTAATCCAAAACACATGTCCAACATGTCATGCTAATTTCTTAGGCTTGGCCACTAACCTCTACTGCCCAAAACTTATTATCGGCTAGAACTCTACTTCTTCTTTAGAGCCTTATAACTTAAGATTCAGAATTCAAATGAAAATACTTTCTTCTACAAAGTTGTTTCAAAATGTCTTAACTTTCTTACTGCAAACTTTTAGATCAAGACACCAAACTATGAGTTATATAGCCTTTTGGGAGTGCATCTCACTCATATTTACTCGCGAACTGACCTTCTCGCATTCTCTTATCCAAAACGCCAACTTTCTCATATCAAAAACGCCCAGCCGCCTTCCTCAATAAACTAGACTCATTTTTTGAACTTTCAAGACCAGTTTTTTTGAAATTGCTCAAGCAAGTGAGTTGTGAGAAACTCTTTTTCGAAGTTAGCTAAAACGTTCAATCTCTATTATCCTTCTTTCTTGCCAACCTTAAACACTCAAAACGCCTAAAATACTATTCCTTGATACTTAATAAAAAAAAATTCCTTCTTTCAAACCTCTTAAACTCTTTGAAAAATTTACACTTCGAAAAATTTACACTACCTAAAGTTTGGGTCTTACATGTACTATTAAAGGGAAGATTGAAAATTAACTATGTTAAGATATATATCAATTTATAACCTTCAATTTTGGATGGTATAATTAAAATGAACATTCTCAAATATAGCAAAACAAAATTATTGTTGCATTGATAGAATTTTAAGTTTTTTTTTTTTTTTTGTATTATTTTGTATATATTTTTCAATAATTTTTCTAATTAAAATTATCAACTAATAATTATATTAATTTAAATATCGAAGAAGAATTTGTATCAAACTTTTGTAATCATATCAATTTAATTTTATACTTCTTACATCGCCAATTGAACAAGAACGTAACACAATCGACCTTTAACGTTGTATTATTTAAACACATACTTGTTTTTTTTTTTTTTGTGGGATTGAATGAATGTTGTGTAAATGTTGATATGAGTGAATACTTTTGAAAAAAATTGTATTAAAAAAATAAATTTAAATCACTAAATAAATATTGATTGAACAAACATGCATTTAATAAATAATTAAGAGGGAAAATAATGAATTAATGAATTGTTGTTGAAATGGAAAACAGTCAGTGGACTTGGGGGCAGAGTTGATTAAGCTCACAAACAACAGCATCTGCAGAATGATGATGAGCACAAGATGTTCCGAAGAGAACAACGAAGCAGAGAAGATCAGAATTTTGGTTAACGACACCATTGAAATTGCTACGAAGATGGCTTTCGGAGATCTCTTCACCCAAGGTCCATTGAAGAGGCTTCCCTTCTGGCTCTTTGGCAACAAAGCTCTCCAAATCAATGTCCGATTCGACCTTCTCCTTGAAAACATTCTGCAACAACACGAACAACGAGCCAAAATCCACGGCCTCGAAAGAGAAGACTGCGATCTCATGGATATATTACTCAAAGCATATTTGGATGAGAAAGCCGAGTTCAAAATGACTAGAAATCACATCAAAGCCTTCTTACTTGTGAGTTCCATTTGTAAACCATTCTAATTCTTTGTTTCCTCTGTTCTAAATCCTAATATTATAAGACTCTCTTGTATCTCTCGACTGACCTTTCGATTGATCAAGTACCAACACCCTTACTTAGAAGCACCATTGTTTTGTGTTATGTAGCATTTTTTGTTTCGGGATTTCGTAACAACTAACATTTGATTTATTCCTCTTAGGATCTGTTCATTGCCGGCACCGGAACGTCGGCTGAGGTTATGCAGTGGGCAATGGCGGAACTAATGAACCATCCGGATGTGTTCCAGAAGGTGCGGCGAGAGATCGAATCAGTCGCCGGGACGAGACTTGTGGAAGAAACGGATGTTACAAATCTTCCGTACACGCAGGCGGTAGTGAAGGAGTGTCTCCGGCTGTATCCAGCGGTGCCGGTGGCGAGACGAGCGTGCCGAGAAACTTGCAAGGTGAATGGATACGATATTCCAAAGGACATCATGGTTGCAGTGGACCTGTTTGCGATAATGCGGGATCCAAATCTGTGGGAGAATCCAGACGAATTTCGGCCTGAGAGATTCTACAATGAGAATTCCAGTAAAGAAGAAGGAACGAAACACATTCAATATGAGATTAAGGGACAGAGCTTCAGCTTTGTGCCATTCGGAGGAGGAAGAAGGGGATGCCCGGGGTCATTGTTAGCGTTCAACACGATCAATAGGACGGTGGCGGCATTGGTTCAGTGCTTTGATTGGAAGGTTGGGAAAGATGGAGATGAAGAGAAGGTGAATATGGAGATTGGAACAGGAATTAGTTTGCCAATGGCTCATCCATTGATCTGTGTGCCTGTTTCTCATTCAACTCCTTTTGTTGCTCAGTAAAGCCATCGACCTCATTGTGACAATCTGCCATGGGAGGTTTGGCCTTCTCTATTCTTAGACTTTTTCATCATGTGTTCTTAAGGTTTTTTTTTGGTTTGTACTGAACAAATGCATATTAATAACTATTAATAATGGAATTCAAGAATATCATGAATGTATAATCATACTCAGTGCATCAATCTTTGTTCATTTCTTTCATCACTCACGCTAAAAAATGGTTTAAATATTTTTAAAAAAGAAAAAACGATTGAATATTTTAAAGAAATGTATGGAGAGTGGTGGATCTCAATATAAAAAAAATTAGTTGTAGCCACCCCTCCAGTAGGAGTTGTTGAATTCAAAAAACTTTATAACACTTTTTTATAATGTAAATATGACTAACTAATTAGATATATACTACTATCAAAAGGCTATTGGATCACTATCGGTTTTAATTTTGCTATTTTGTAATTTAGAAAATTTAATGACTCTAATATAAAAAAAAAAAAAAGATATACTGTCTTTTTGTAAACGCTCCGATTGTTTTCTTGACATCAAGATTAATGTTTGTTTGAGATCAATCAAGTAAAATTCAACCGTGAACAAATCTTAACTGATACGAATAAATCATCTATGTAATGGAAAATTTGGTATCTTTCTCTAGCATCTTTAAAAAAAATTATAAATATGGTTTTTTCTCTTTTTTATCTTTTCACTTCTCAATTCTTAATACCAATTTTTTAAAAAATTTGTATAAAATAATTGAGATAAAAATGAAGTTTATTTTTAATTTAAAAGCATATAAAATTTTTAAATAGTTATGTTAGTAGTAAGTTTGAGATTGTGAATATCGATATACAATTGATATAATATGTTTAGGTTGATCATTTATACTAAATCAAAACAATTTCCTACTTCTTTAATCAAAAACCTCATTATGCTCTTTTGACTTTAATATTGAAATTTAGCAAATGTTTTTTACTATATGTTGCCATTCATTTTTCTCAAATTATTATTATAATCAATTATTTTGAAAGCTACAACAATTTCAAATTCAGATTCCAACTATTAAGTAATTCTCAAATTTAAATTTAATATATTTAACTTAGTTTAATTTTCAAAAACCAAAAACTTTATCCATTGAACTTTTAATTTAACTGAAAAAAAGAACAAAAATGTAATTTGATATTATATTTTTTCCTTTTTATCTAGTGGCTACACAAAACAGAAAGGTTAATTGAAAAAGGTGAGTACATAATAAACTAAAAATTAAAGTTCAGAATTCTATTAAGCATAATTTTAAAATTTATGTCTACTAGTCCATTAAAAATCTATTAAACATGAAGTTGAAAATCAAGGTACTGTTTGTAACAATTTTGTTTTTGTTTTTGTTTTTTTCTCCATTGTTTGCAACTTATCTTTGTTAAGTATTAAAGTTAAATTTTTATTCAAAATCTAAAAGCAAAAACTTTTTTTTATTTTTAAAATTTGACTTCCATTTTGATAACATTGGTAAAAATTTGACAAACACAAAATGCAAAAAATTTTTGAGGTGGAATGAATGTTGATAGATTTAAGTTTAAAAACAAAACAATTGCCTAAGGGGTCTAAAAAACTTTAAACATAAAATTGAAAGCTCAAGACTTATCAAATAACAAAATGAAAGTTTAGGGATCAAATAGATGCACAAATTAGTTTTAGTTTGAATGTTCATAGACTAAACTTGTAACTTTTTGTCCTTACTACCTACCTACCTCCTCGTGTGTTGTATCATCTCATTTAGAATTACTAATTCATTGTAAAAAAAAAACAGAAATTTTTTCATGGCTGGTACAGATACTTCATCAGTAGCCATCCAGCGTGTAATAGCTGAACTAATCAACCATTCTGAAATGAGATAGAATTTGTGGTTGGAACATCAGGAAAACTTGTGGAAGAGGAAGACATTCCTCAACACCCTTATTTGCAAGCTGTTGTCAAGGAAACTTTGAGGCTCTACCCATCAGTACCAATCAACATAAGAGAATGTTGTCAAAGTTGCAAGATTGGAGGCTACGACGTGCCACAAGAAACAACAGTTGCAATCAATCTCTTTGCAATTAATTATGAGAGACACTCGAGTGTGGAGTGAACCTAATGAATTTCGTCCATGGAGGTATTTGGCTTTGTCATCTTAAAAATATCAAATTGAGACCAATAAGGGATACAGTTTCGACTGAATACAACAACTGCTACTCTGGTTCAGTGCTTTGATTGAAAGGTGGTTGGAGATGGGGAAGAAACTAAGGTTGATAGGCAACCAGGACAAGGGTTGACTTTAGGAATGGCCAACCCTCTTTTGCTTTTTCCTATGTTACATTTTGATCCATTTGGTGCTTCAATGTAAACAGTTCATGATCA

Coding sequence (CDS)

ATGCCGGACGCCGCTCTTCACTACACTCTCTACCTTCTCCTCTTCTTCCTCTCCTCTCTCCTTCTCTTCTTCTTCCTCCACAAACCCTCCAAATCCGCCTCCGCAAATCTCACTCTACCTCCATCTCCACCGCCGCTCCCAGTCATCGGCCACCTCCATCTCCTCCTCCCTGCCACTCACAAATCCTTCTTCAACATCTCCTCTAAGTACGGTCCGCTCCTCCACATCCGCCTCGGCGCTCTGCAGTACGTCCTCGTCTCCTCCGCCTCCCTCGCTGCTGAGATCTTCAAAACGCACGATCTCACCTTCTCCTCCCGACCTGATTTCGCCTTCAGCGAGGAGTATCCGTACGGAAAAGTGGGATTCCTTGGCGCTCCGTACGGGGATTATTGGCGGTATATGAAGAAGCTGACGATGATGGAGGTTCTTGCGGCGCCACAGCTTGCACGATCGCGATTTGTGAGGAATGAAGAGATCCTTCGTATGCTTCAAAAGTTGCTGCTCTGTTCCAAGCAGAAGCAGTCAGTGGACTTGGGGGCAGAGTTGATTAAGCTCACAAACAACAGCATCTGCAGAATGATGATGAGCACAAGATGTTCCGAAGAGAACAACGAAGCAGAGAAGATCAGAATTTTGGTTAACGACACCATTGAAATTGCTACGAAGATGGCTTTCGGAGATCTCTTCACCCAAGGTCCATTGAAGAGGCTTCCCTTCTGGCTCTTTGGCAACAAAGCTCTCCAAATCAATGTCCGATTCGACCTTCTCCTTGAAAACATTCTGCAACAACACGAACAACGAGCCAAAATCCACGGCCTCGAAAGAGAAGACTGCGATCTCATGGATATATTACTCAAAGCATATTTGGATGAGAAAGCCGAGTTCAAAATGACTAGAAATCACATCAAAGCCTTCTTACTTGATCTGTTCATTGCCGGCACCGGAACGTCGGCTGAGGTTATGCAGTGGGCAATGGCGGAACTAATGAACCATCCGGATGTGTTCCAGAAGGTGCGGCGAGAGATCGAATCAGTCGCCGGGACGAGACTTGTGGAAGAAACGGATGTTACAAATCTTCCGTACACGCAGGCGGTAGTGAAGGAGTGTCTCCGGCTGTATCCAGCGGTGCCGGTGGCGAGACGAGCGTGCCGAGAAACTTGCAAGGTGAATGGATACGATATTCCAAAGGACATCATGGTTGCAGTGGACCTGTTTGCGATAATGCGGGATCCAAATCTGTGGGAGAATCCAGACGAATTTCGGCCTGAGAGATTCTACAATGAGAATTCCAGTAAAGAAGAAGGAACGAAACACATTCAATATGAGATTAAGGGACAGAGCTTCAGCTTTGTGCCATTCGGAGGAGGAAGAAGGGGATGCCCGGGGTCATTGTTAGCGTTCAACACGATCAATAGGACGGTGGCGGCATTGGTTCAGTGCTTTGATTGGAAGGTTGGGAAAGATGGAGATGAAGAGAAGGTGAATATGGAGATTGGAACAGGAATTAGTTTGCCAATGGCTCATCCATTGATCTGTGTGCCTGTTTCTCATTCAACTCCTTTTGTTGCTCAGTAA

Protein sequence

MPDAALHYTLYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLLPATHKSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKVGFLGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGAELIKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFWLFGNKALQINVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRNHIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGTRLVEETDVTNLPYTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEFRPERFYNENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQCFDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVAQ
Homology
BLAST of Cucsat.G12140.T2 vs. ExPASy Swiss-Prot
Match: Q9LJY5 (Cytochrome P450 705A22 OS=Arabidopsis thaliana OX=3702 GN=CYP705A22 PE=2 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 1.7e-120
Identity = 229/514 (44.55%), Postives = 338/514 (65.76%), Query Frame = 0

Query: 10  LYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLLPA-THKSFFNISS 69
           +++L+F L+ L  FFF  KP K +  N  LPPSPP LP+IGH+HLLL   THKS   +SS
Sbjct: 14  IFILIFLLTFLCFFFFFKKP-KDSRVNFDLPPSPPSLPIIGHVHLLLSTLTHKSLQKLSS 73

Query: 70  KYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKVGFLGAPYG 129
           +YGPLL++R+  +  +LVSSAS+A EIF+T D+  SSR   A  E   +G   F+ APYG
Sbjct: 74  RYGPLLYLRIFNVPIILVSSASVAYEIFRTQDVNISSRGVTAVDESLVFGSSSFVTAPYG 133

Query: 130 DYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGAELIKLTNN 189
           DYW++MKKLT+M++L      +SR +R ++I R  + LL  +++K+SV++G E + L NN
Sbjct: 134 DYWKFMKKLTVMKLLGPQAQEQSRDIRADDIKRFCRNLLDKARKKESVEIGKEAMNLMNN 193

Query: 190 SICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFWLFGNKALQ 249
            +C+M M    SEEN E EK+R LV ++I +  KM F  +  +  L++L   LF    + 
Sbjct: 194 ILCKMSMGRSFSEENGETEKLRGLVTESIGLMKKM-FLAVLLRRQLQKLGISLFKKDIMG 253

Query: 250 INVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRNHIKAFLLD 309
           ++ +FD+LLE +L +H ++ +    + +   ++D+LL AY DE AE+K+T+NHIKAF +D
Sbjct: 254 VSNKFDVLLEKVLVEHREKPE----KDQGTVMLDVLLAAYGDENAEYKITKNHIKAFFVD 313

Query: 310 LFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAG-TRLVEETDVTNLPYTQAVVK 369
           LFI  T TS + +QW MAE+MN+  + +++R EI+SV G +RL++ETD+ NLPY  AV+K
Sbjct: 314 LFIGATDTSVQTIQWTMAEIMNNTHILERMREEIDSVVGKSRLIQETDLPNLPYLHAVIK 373

Query: 370 ECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEFRPERFYN 429
           E LRL+P  P+  R  ++ CK+ G+ IP+   + ++ + +MRDPN+WE+P+EF+PERF  
Sbjct: 374 EALRLHPPGPLLPREFQQGCKIGGFYIPEKTTLLINAYVVMRDPNVWEDPEEFKPERFLA 433

Query: 430 ENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQCFDWKVGK 489
            + S +E       E + Q+  F+PFG GRRGCPGS LA+  +   +  +VQCFDW++  
Sbjct: 434 SSRSGQED------ERREQALKFLPFGSGRRGCPGSNLAYMIVGSAIGMMVQCFDWRI-- 493

Query: 490 DGDEEKVNM-EIGTGISLPMAHPLICVPVSHSTP 521
             + EKVNM E   G  L MAHPL   PV+   P
Sbjct: 494 --EGEKVNMKEAVKGTILTMAHPLKLTPVTRQPP 511

BLAST of Cucsat.G12140.T2 vs. ExPASy Swiss-Prot
Match: Q9FI39 (Cytochrome P450 705A5 OS=Arabidopsis thaliana OX=3702 GN=CYP705A5 PE=2 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 2.1e-118
Identity = 236/508 (46.46%), Postives = 328/508 (64.57%), Query Frame = 0

Query: 10  LYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLL-PATHKSFFNISS 69
           ++LLL   S L    F  K +K + A   LPPSPP LP+IGHLHL+L    H+SF NISS
Sbjct: 14  IFLLLCLFSRLSYDLFFRK-TKDSRAGCALPPSPPSLPIIGHLHLILFVPIHQSFKNISS 73

Query: 70  KYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKVGFLGAPYG 129
           KYGPLLH+R      VLVSSAS A EIFK  D+  SSRP     E    G   F+  PYG
Sbjct: 74  KYGPLLHLRFFNFPIVLVSSASTAYEIFKAQDVNVSSRPPPPIEESLILGSSSFINTPYG 133

Query: 130 DYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGAELIKLTNN 189
           DY ++MKK  + ++L    L RSR +R +E+ R  + LL  + +KQ+V++  E +KLTNN
Sbjct: 134 DYSKFMKKFMVQKLLGPQALQRSRNIRADELERFYKTLLDKAMKKQTVEIRNEAMKLTNN 193

Query: 190 SICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFWLFGNKALQ 249
           +IC+M+M   CSEEN EAE +R LV ++I +  K   G +F + PLK+L   LF  + + 
Sbjct: 194 TICKMIMGRSCSEENGEAETVRGLVTESIFLTKKHFLGAMFHK-PLKKLGISLFAKELMN 253

Query: 250 INVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRNHIKAFLLD 309
           ++ RFD LLE IL +HE++ + H    +  D++D+LL+AY DE AE+K+TR+ IK+  +D
Sbjct: 254 VSNRFDELLEKILVEHEEKLQEH---HQTSDMLDMLLEAYGDENAEYKITRDQIKSLFVD 313

Query: 310 LFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAG-TRLVEETDVTNLPYTQAVVK 369
           LF AGT  SA  +QW MAE++ +P + +++R EI+SV G TRLV+ETD+ NLPY QA+VK
Sbjct: 314 LFSAGTEASANTIQWTMAEIIKNPKICERLREEIDSVVGKTRLVQETDLPNLPYLQAIVK 373

Query: 370 ECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEFRPERFYN 429
           E LRL+P  PV  R  +ETC++ G+ IP+   + V+++AIMRDP+ WE+P+EF+PERF  
Sbjct: 374 EGLRLHPPGPVV-RTFKETCEIKGFYIPEKTRLFVNVYAIMRDPDFWEDPEEFKPERFLA 433

Query: 430 ENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQCFDWKVGK 489
            +   EE  K      +     ++PFG GRR CPGS LA+  +   +  +VQ FDW +  
Sbjct: 434 SSRLGEEDEK------REDMLKYIPFGSGRRACPGSHLAYTVVGSVIGMMVQHFDWII-- 493

Query: 490 DGDEEKVNMEIGTGISLPMAHPLICVPV 516
               EK+NM+ G  ++L MAHPL C PV
Sbjct: 494 --KGEKINMKEGGTMTLTMAHPLKCTPV 505

BLAST of Cucsat.G12140.T2 vs. ExPASy Swiss-Prot
Match: O81973 (Cytochrome P450 93A3 OS=Glycine max OX=3847 GN=CYP93A3 PE=2 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 2.1e-110
Identity = 217/489 (44.38%), Postives = 304/489 (62.17%), Query Frame = 0

Query: 39  LPPSPPPLPVIGHLHLLLPATHKSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKT 98
           LPPSP PLP+IGHLHLL P  H+ F  +S +YGP++H+ LG++  V+ S+A  A E  KT
Sbjct: 31  LPPSPMPLPIIGHLHLLSPTPHQDFHKLSLRYGPIIHLFLGSVPCVVASTAEAAKEFLKT 90

Query: 99  HDLTFSSRP-DFAFSEEYPYGKVGFLGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNE 158
           H+  FS+RP +    E   YG   FL APYG YW++MKKL M E+L    L +   VR  
Sbjct: 91  HEPAFSNRPANTVAVETLTYGFQDFLFAPYGPYWKFMKKLCMSELLGGHMLDQFLPVRQX 150

Query: 159 EILRMLQKLLLCSKQKQSVDLGAELIKLTNNSICRMMMS-TRCSEENNEAEKIRILVNDT 218
           E  + ++++L      ++VD G E I L+NN + RM++S T  +E+ NE E++R LV D 
Sbjct: 151 ETKKFIKRVLQKGISGEAVDFGGEFITLSNNIVSRMIVSQTSTTEDENEVEEMRKLVKDA 210

Query: 219 IEIATKMAFGDLFTQGPLKRLPFWLFGNKALQINVRFDLLLENILQQHEQRAKIHGL--- 278
            E++ K    D  +   LKR     F  +  +I   FD +L+ I++Q E+  +       
Sbjct: 211 AELSGKFNISDFVSF--LKRFDLQGFNKRLEKIRDCFDTVLDRIIKQREEERRNKNETVG 270

Query: 279 EREDCDLMDILLKAYLDEKAEFKMTRNHIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPD 338
           +RE  D++D+L     DE +E K+ + +IKAF+LD+ IAGT TSA  M+WAMAEL+N+P 
Sbjct: 271 KREFKDMLDVLFDISEDESSEIKLNKENIKAFILDILIAGTDTSAVTMEWAMAELINNPG 330

Query: 339 VFQKVRREIESVAG-TRLVEETDVTNLPYTQAVVKECLRLYPAVPVARRACRETCKVNGY 398
           V +K R+E+++V G +R+VEE+D+ NLPY Q +V+E LRL+PA P+  R       V GY
Sbjct: 331 VLEKARQEMDAVVGKSRIVEESDIANLPYLQGIVRETLRLHPAGPLLFRESSRRAVVCGY 390

Query: 399 DIPKDIMVAVDLFAIMRDPNLWENPDEFRPERFYNENSSKEEGTKHIQYEIKGQSFSFVP 458
           DIP    + V+++AI RDPN WENP EFRPERF     S        Q +++GQ +  +P
Sbjct: 391 DIPAKTRLFVNVWAIGRDPNHWENPLEFRPERFVENGKS--------QLDVRGQHYHLLP 450

Query: 459 FGGGRRGCPGSLLAFNTINRTVAALVQCFDWKVGKDGDEEKVNMEIGTGISLPMAHPLIC 518
           FG GRR CPG+ LA   ++  +A L+QCF WKV  D D  KVNME   GI+LP AHP+IC
Sbjct: 451 FGSGRRACPGTSLALQVVHVNLAVLIQCFQWKV--DCDNGKVNMEEKAGITLPRAHPIIC 507

Query: 519 VPVSHSTPF 522
           VP+    PF
Sbjct: 511 VPIRRLNPF 507

BLAST of Cucsat.G12140.T2 vs. ExPASy Swiss-Prot
Match: Q9LJY7 (Cytochrome P450 705A20 OS=Arabidopsis thaliana OX=3702 GN=CYP705A20 PE=2 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 1.0e-109
Identity = 228/518 (44.02%), Postives = 323/518 (62.36%), Query Frame = 0

Query: 8   YTLYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLLPA-THKSFFNI 67
           ++  LL FF  SLL +  L K  K +     L  SPP LP+IGHLH LL +  HKS   +
Sbjct: 10  FSFILLCFF--SLLCYSLLFKKLKDSHVGRDLLQSPPSLPIIGHLHHLLSSLAHKSLQQL 69

Query: 68  SSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKVGFLGAP 127
           SSKYGPLLH+ +     VLVSSAS+A EIFK HDL  SSR +   +E    G   F+GAP
Sbjct: 70  SSKYGPLLHLSIFNFPVVLVSSASVAYEIFKAHDLNISSRDNPPINESLLVGSSVFVGAP 129

Query: 128 YGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGAELIKLT 187
           YGDYW++MKKL + ++L    L RSR +R +E+ R  + LL  + +K+SV++G E  KL+
Sbjct: 130 YGDYWKFMKKLLVTKLLGPQALERSRSIRADELERFYRSLLDKAMKKESVEIGKEATKLS 189

Query: 188 NNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFWLFGNKA 247
            NSICRM M    SEE+ EAE++R LV +   +  K+   ++  + PL++L   LF  + 
Sbjct: 190 INSICRMSMGRSFSEESGEAERVRGLVTELDGLTKKVLLVNIL-RWPLEKLRISLFKKEI 249

Query: 248 LQINVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRNHIKAFL 307
           + ++  FD LLE I+ + E++      E +   LMD+LL+AY DEKAE K+TRNHIK+  
Sbjct: 250 MYVSNSFDELLERIIVEREKKPN----EHQGTYLMDVLLEAYEDEKAEHKITRNHIKSLF 309

Query: 308 LDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAG-TRLVEETDVTNLPYTQAV 367
           ++L + GT TSA+ +QW MAEL+N+ +V +++R EI+SV G TRL++E D+  LPY Q+V
Sbjct: 310 VELLLGGTDTSAQTIQWTMAELINNRNVLKRLREEIDSVVGETRLIQEKDLPKLPYLQSV 369

Query: 368 VKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEFRPERF 427
           VKE LRL+P +P+  R  + +C++ G+ I +   + V+ +A+MRDP  WE+PDEF+PERF
Sbjct: 370 VKEGLRLHPPLPLMVRTFQRSCEMKGFYIAEKTTLVVNAYAVMRDPTTWEDPDEFKPERF 429

Query: 428 YNENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQCFDWKV 487
             +   +    KHI             FG GRRGCPGS LA   I   +  +VQCFD  +
Sbjct: 430 LRQEEER-RALKHI------------AFGSGRRGCPGSNLATIFIGTAIGTMVQCFDLSI 489

Query: 488 GKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVA 524
             D    KV M+   G++L MAHPL C+ V  + PF++
Sbjct: 490 KGD----KVKMDEVGGLNLTMAHPLECILVPRTQPFIS 503

BLAST of Cucsat.G12140.T2 vs. ExPASy Swiss-Prot
Match: Q9FH67 (Cytochrome P450 705A12 OS=Arabidopsis thaliana OX=3702 GN=CYP705A12 PE=3 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 4.8e-107
Identity = 221/517 (42.75%), Postives = 321/517 (62.09%), Query Frame = 0

Query: 9   TLYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLLPAT--HKSFFNI 68
           ++++LL   S L    F  KP         LPPSPP LP+IGHLH LL ++  HKSF  +
Sbjct: 13  SIFILLCLFSFLCYALFFKKPK-----GFDLPPSPPSLPIIGHLHHLLSSSLPHKSFQKL 72

Query: 69  SSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKVGFLGAP 128
           S KYGPLLH+R+     VLVSSAS+A E+F+T+D+  S R      +   +G  GF+ AP
Sbjct: 73  SFKYGPLLHLRIFNFPMVLVSSASMAYEVFRTNDVNVSYRFVPVNKDSLVFGSSGFVTAP 132

Query: 129 YGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGAELIKLT 188
           YGDYW++MKKL   ++L    L  S+  R EE+ R    L   +++K+SV++G   +KLT
Sbjct: 133 YGDYWKFMKKLISTKLLRPHALELSKGNRAEELRRFCLDLQGKARKKESVEIGKVALKLT 192

Query: 189 NNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFWLFGNKA 248
           NN ICRM M   CSE+N  AE+ R LVN +  ++ K+ F ++F +               
Sbjct: 193 NNIICRMSMGRSCSEKNGVAERARELVNKSFALSVKLFFSNMFRK-------------DI 252

Query: 249 LQINVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRNHIKAFL 308
           + ++  FD  LE IL +HE+  +      +D D++D LL+AY +E+AE+K+TR  IK+ +
Sbjct: 253 MGVSREFDEFLERILVEHEENLE----GDQDRDMIDHLLEAYRNEEAEYKITRKQIKSLI 312

Query: 309 LDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIES-VAGTRLVEETDVTNLPYTQAV 368
           +++F+ GT +SA+ +QW MAE++N+P V +K+R EI+S V G RL++E+D+ NLPY QAV
Sbjct: 313 VEIFLGGTDSSAQTIQWTMAEILNNPGVLEKLRAEIDSVVGGKRLIQESDLPNLPYLQAV 372

Query: 369 VKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEFRPERF 428
           VKE LRL+P+ PV  R   E+C+V  + +P+   + V+L+A+ RDP+ WE+PD F+PERF
Sbjct: 373 VKEGLRLHPSAPVLLRVFGESCEVKEFYVPEKTTLVVNLYAVNRDPDSWEDPDMFKPERF 432

Query: 429 YNENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQCFDWKV 488
              + S +E       +I+ Q+  +V FGGGRR CP   LA   +   + A+VQCFDW++
Sbjct: 433 LVSSISGDE------EKIREQAVKYVTFGGGRRTCPAVKLAHIFMETAIGAMVQCFDWRI 492

Query: 489 GKDGDEEKVNMEIG-TGISLPMAHPLICVPVSHSTPF 522
                 EKV ME   +G+SL MAHPL C PV    PF
Sbjct: 493 ----KGEKVYMEEAVSGLSLKMAHPLKCTPVVRFDPF 497

BLAST of Cucsat.G12140.T2 vs. NCBI nr
Match: NP_001292616.1 (cytochrome P450 705A5-like [Cucumis sativus] >AIT72034.1 cytochrome P450 [Cucumis sativus] >KGN58608.1 hypothetical protein Csa_002418 [Cucumis sativus])

HSP 1 Score: 1041 bits (2692), Expect = 0.0
Identity = 522/524 (99.62%), Postives = 523/524 (99.81%), Query Frame = 0

Query: 1   MPDAALHYTLYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLLPATH 60
           MPDAALHYTLYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLLPATH
Sbjct: 1   MPDAALHYTLYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLLPATH 60

Query: 61  KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV 120
           KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV
Sbjct: 61  KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV 120

Query: 121 GFLGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGA 180
           GFLGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGA
Sbjct: 121 GFLGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGA 180

Query: 181 ELIKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFW 240
           ELIKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFT+GPLKRLPFW
Sbjct: 181 ELIKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTRGPLKRLPFW 240

Query: 241 LFGNKALQINVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRN 300
           LFGNKALQINVRFDLLLENILQQHEQRAKIHGLERED DLMDILLKAYLDEKAEFKMTRN
Sbjct: 241 LFGNKALQINVRFDLLLENILQQHEQRAKIHGLEREDRDLMDILLKAYLDEKAEFKMTRN 300

Query: 301 HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGTRLVEETDVTNLP 360
           HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGTRLVEETDVTNLP
Sbjct: 301 HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGTRLVEETDVTNLP 360

Query: 361 YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF 420
           YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF
Sbjct: 361 YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF 420

Query: 421 RPERFYNENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQC 480
           RPERFYNENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQC
Sbjct: 421 RPERFYNENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQC 480

Query: 481 FDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVAQ 524
           FDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVAQ
Sbjct: 481 FDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVAQ 524

BLAST of Cucsat.G12140.T2 vs. NCBI nr
Match: XP_008461786.1 (PREDICTED: cytochrome P450 705A5-like [Cucumis melo] >KAA0048327.1 cytochrome P450 705A5-like protein [Cucumis melo var. makuwa] >TYK10670.1 cytochrome P450 705A5-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1014 bits (2623), Expect = 0.0
Identity = 506/524 (96.56%), Postives = 512/524 (97.71%), Query Frame = 0

Query: 1   MPDAALHYTLYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLLPATH 60
           MPDAALHY LYLLLFF SSLLLFFFLHKPSKSA  NLTLPPSPPPLPVIGHLHLLLPATH
Sbjct: 1   MPDAALHYALYLLLFFFSSLLLFFFLHKPSKSAPPNLTLPPSPPPLPVIGHLHLLLPATH 60

Query: 61  KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV 120
           KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV
Sbjct: 61  KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV 120

Query: 121 GFLGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGA 180
           GFLGAPYGDYWRYMKKLTMMEVLA PQLARSRFVRNEEILRMLQKLLLCSKQK+SVDLGA
Sbjct: 121 GFLGAPYGDYWRYMKKLTMMEVLAPPQLARSRFVRNEEILRMLQKLLLCSKQKESVDLGA 180

Query: 181 ELIKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFW 240
           EL+KLTNNSICRMMMSTRCSEENNEAEKIR LVNDTIEIATKMAFGDLF QGPLKRLPFW
Sbjct: 181 ELVKLTNNSICRMMMSTRCSEENNEAEKIRTLVNDTIEIATKMAFGDLFGQGPLKRLPFW 240

Query: 241 LFGNKALQINVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRN 300
           LFG KAL+INVRFD+LLENILQQHEQRAKIHGLERED DLMDILLKAYLDEKAEFKMTRN
Sbjct: 241 LFGKKALEINVRFDVLLENILQQHEQRAKIHGLEREDRDLMDILLKAYLDEKAEFKMTRN 300

Query: 301 HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGTRLVEETDVTNLP 360
           HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESV GTRLVEETDVTNLP
Sbjct: 301 HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVVGTRLVEETDVTNLP 360

Query: 361 YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF 420
           YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKD MVAVDLFAIMRDPNLWENPDEF
Sbjct: 361 YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDTMVAVDLFAIMRDPNLWENPDEF 420

Query: 421 RPERFYNENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQC 480
           RPERFYNENSSKEEGTKHIQYEIKGQ+FSFVPFGGGRRGCPGSLLAFNTINRTVAALVQC
Sbjct: 421 RPERFYNENSSKEEGTKHIQYEIKGQNFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQC 480

Query: 481 FDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVAQ 524
           FDWKVGKDGDEEKVNMEIG+GISLPMAHPLICVPVSHSTPFVA 
Sbjct: 481 FDWKVGKDGDEEKVNMEIGSGISLPMAHPLICVPVSHSTPFVAH 524

BLAST of Cucsat.G12140.T2 vs. NCBI nr
Match: XP_038903604.1 (cytochrome P450 705A22-like [Benincasa hispida])

HSP 1 Score: 941 bits (2433), Expect = 0.0
Identity = 468/527 (88.80%), Postives = 494/527 (93.74%), Query Frame = 0

Query: 1   MPDAALHYTLYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLLPATH 60
           M DA+LHY LYLLLFFLS L LFFFLHKPSKSA   +TLP SPP LPVIGHLHLLLPA H
Sbjct: 1   MADASLHYALYLLLFFLSFLFLFFFLHKPSKSAPGKITLPSSPPSLPVIGHLHLLLPANH 60

Query: 61  KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV 120
           KSF NISSKYGPLLHIRLGALQYVLVSSASLAA+IFKT DL FSSRPDFAFSEEYPYGKV
Sbjct: 61  KSFINISSKYGPLLHIRLGALQYVLVSSASLAADIFKTQDLAFSSRPDFAFSEEYPYGKV 120

Query: 121 GFLGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGA 180
           GFLGAPYGDYWRYMKKLTMME+L+ PQLARSRFVR+EEI RML+ LLLCSK+K+SVDLGA
Sbjct: 121 GFLGAPYGDYWRYMKKLTMMELLSPPQLARSRFVRDEEITRMLRDLLLCSKKKESVDLGA 180

Query: 181 ELIKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFW 240
            LIKLTNNSICRMMMSTRCSE+NNEAEKIRILVNDTIEIATK+AFGDLF+ GPLKRLPFW
Sbjct: 181 MLIKLTNNSICRMMMSTRCSEQNNEAEKIRILVNDTIEIATKLAFGDLFSHGPLKRLPFW 240

Query: 241 LFGNKALQINVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRN 300
           LFG K L IN++FD+LLENILQQHEQRAKIHGL+R+D DLMDILLKAYLDEKAEFKMTRN
Sbjct: 241 LFGKKTLDINLKFDVLLENILQQHEQRAKIHGLDRDDRDLMDILLKAYLDEKAEFKMTRN 300

Query: 301 HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGTRLVEETDVTNLP 360
           HIKAFLLDLFIAGTGTSAEVMQW MAEL+NHPDVFQKVRREIESV GTRLVEETD+TNLP
Sbjct: 301 HIKAFLLDLFIAGTGTSAEVMQWTMAELINHPDVFQKVRREIESVIGTRLVEETDITNLP 360

Query: 361 YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF 420
           Y QAVVKECLRLYPAVPVARRACRETCKVNG+DIPK IMVAVDLFAIM DPNLWENPDEF
Sbjct: 361 YMQAVVKECLRLYPAVPVARRACRETCKVNGFDIPKGIMVAVDLFAIMHDPNLWENPDEF 420

Query: 421 RPERFYNENSSKEEG----TKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAA 480
           RPERFYN NS K+EG    T+HIQYEIKGQ+FSFVPFGGGRRGCPGSLLA+NTINRTVAA
Sbjct: 421 RPERFYNTNSGKDEGDHQGTRHIQYEIKGQNFSFVPFGGGRRGCPGSLLAYNTINRTVAA 480

Query: 481 LVQCFDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVA 523
           LVQCFDWKVGKDG+EE+VNMEIGTGISLPMAHPLICVPVSHSTPFVA
Sbjct: 481 LVQCFDWKVGKDGNEERVNMEIGTGISLPMAHPLICVPVSHSTPFVA 527

BLAST of Cucsat.G12140.T2 vs. NCBI nr
Match: XP_022949336.1 (cytochrome P450 705A22-like [Cucurbita moschata])

HSP 1 Score: 903 bits (2334), Expect = 0.0
Identity = 453/526 (86.12%), Postives = 484/526 (92.02%), Query Frame = 0

Query: 8   YTLYLLLFFLSSLLLFFFLHKPSKSAS-------ANLTLPPSPPPLPVIGHLHLLLPATH 67
           Y LY LLFFLS L L FFLHK S SAS       A LTLPPSPP LP+IGHLHLLLPA +
Sbjct: 4   YILYFLLFFLSCLFLLFFLHKTSGSASSSSSAAAAKLTLPPSPPSLPLIGHLHLLLPANY 63

Query: 68  KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV 127
           KSFFNISSKYGPLLHIRL +LQYVLVSSASLAAEIF+THDLTFSSRPDFAFSEEYPYGKV
Sbjct: 64  KSFFNISSKYGPLLHIRLCSLQYVLVSSASLAAEIFRTHDLTFSSRPDFAFSEEYPYGKV 123

Query: 128 GFLGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGA 187
           GFLGAPYGDYWRYMKKLTMMEVLA PQLARSRFVRNEEI RML KLLL S++K+SVDLGA
Sbjct: 124 GFLGAPYGDYWRYMKKLTMMEVLAPPQLARSRFVRNEEISRMLHKLLLSSQKKESVDLGA 183

Query: 188 ELIKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFW 247
           EL+KLTNNSICRMMMSTRCSEENNEAE IR LVNDTIEIATK+AFGDLF++GPLKRLPFW
Sbjct: 184 ELVKLTNNSICRMMMSTRCSEENNEAENIRKLVNDTIEIATKLAFGDLFSEGPLKRLPFW 243

Query: 248 LFGNKALQINVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRN 307
           LFG K L++N+RFD+LLE ILQQHE+RAKIHG ER+D DLMDILLKAYLDEKAEFK+TRN
Sbjct: 244 LFGKKVLKLNIRFDVLLEKILQQHEERAKIHGFERDDRDLMDILLKAYLDEKAEFKITRN 303

Query: 308 HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGTRLVEETDVTNLP 367
           HIKAFLLDLFIAGTGTSAEVMQW +AEL+NHP+VF+KVR+EIE V G+RLVEETD+ NLP
Sbjct: 304 HIKAFLLDLFIAGTGTSAEVMQWTIAELINHPNVFRKVRQEIEYVVGSRLVEETDIVNLP 363

Query: 368 YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF 427
           Y QAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF
Sbjct: 364 YMQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF 423

Query: 428 RPERFYNENSSKEEGT---KHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAAL 487
           RPERFYN N++K+EG    KHIQYEIKGQ+FSFVPFGGGRRGCPGSLLA+NTINRTVAAL
Sbjct: 424 RPERFYNANTNKDEGDQGMKHIQYEIKGQNFSFVPFGGGRRGCPGSLLAYNTINRTVAAL 483

Query: 488 VQCFDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVA 523
           VQCFDWKVGKDG+ EKVNMEIGTGISLPMAH  ICVPVSHSTPFVA
Sbjct: 484 VQCFDWKVGKDGNGEKVNMEIGTGISLPMAHAFICVPVSHSTPFVA 529

BLAST of Cucsat.G12140.T2 vs. NCBI nr
Match: XP_022998426.1 (cytochrome P450 705A22-like [Cucurbita maxima])

HSP 1 Score: 902 bits (2330), Expect = 0.0
Identity = 453/524 (86.45%), Postives = 482/524 (91.98%), Query Frame = 0

Query: 8   YTLYLLLFFLSSLLLFFFLHKPSKSAS-----ANLTLPPSPPPLPVIGHLHLLLPATHKS 67
           Y LY LLFFLS L L F LHK S SAS     A LTLPPSPP LP+IGHLHLLLPA +KS
Sbjct: 4   YILYFLLFFLSCLFLLFLLHKTSGSASSSAAAAKLTLPPSPPSLPLIGHLHLLLPANYKS 63

Query: 68  FFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKVGF 127
           FFNISSKYGPLLHIRL +LQYVLVSSASLAAEIF+THDLTFSSRPDFAFSEEYPYGKVGF
Sbjct: 64  FFNISSKYGPLLHIRLCSLQYVLVSSASLAAEIFRTHDLTFSSRPDFAFSEEYPYGKVGF 123

Query: 128 LGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGAEL 187
           LGAPYGDYWRYMKKLTMMEVLA PQLARSRFVRNEEI RML KLLL S++K+SVDLGAEL
Sbjct: 124 LGAPYGDYWRYMKKLTMMEVLAPPQLARSRFVRNEEISRMLHKLLLSSQKKESVDLGAEL 183

Query: 188 IKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFWLF 247
           IKLTNNSICRMMMSTRCSEENNEAE IR LVNDTIEIATK+AFGDLF++GPLKRLPFWLF
Sbjct: 184 IKLTNNSICRMMMSTRCSEENNEAENIRKLVNDTIEIATKLAFGDLFSEGPLKRLPFWLF 243

Query: 248 GNKALQINVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRNHI 307
           G K L++N+RFD+LLE ILQQHE+RAKIHG ER+D DLMDILLKAYLDEKAEFK+TRNHI
Sbjct: 244 GKKVLKLNIRFDVLLEKILQQHEERAKIHGFERDDRDLMDILLKAYLDEKAEFKITRNHI 303

Query: 308 KAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGTRLVEETDVTNLPYT 367
           KAFLLDLFIAGTGTSAEVMQW +AEL+NHP+VF+KVR+EIE V G+RLVEETD+ NLPY 
Sbjct: 304 KAFLLDLFIAGTGTSAEVMQWTIAELINHPNVFRKVRQEIECVVGSRLVEETDIVNLPYM 363

Query: 368 QAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEFRP 427
           QAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEFRP
Sbjct: 364 QAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEFRP 423

Query: 428 ERFYNENSSKEEGT---KHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQ 487
           ERFYN N +K+EG    KHIQYEIKGQ+FSFVPFGGGRRGCPGSLLA+NTINRTVAALVQ
Sbjct: 424 ERFYNANPNKDEGDQGMKHIQYEIKGQNFSFVPFGGGRRGCPGSLLAYNTINRTVAALVQ 483

Query: 488 CFDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVA 523
           CFDWKVGKDG+ EKVNMEIGTGISLPMAH  ICVPVSHSTPFVA
Sbjct: 484 CFDWKVGKDGNGEKVNMEIGTGISLPMAHSFICVPVSHSTPFVA 527

BLAST of Cucsat.G12140.T2 vs. ExPASy TrEMBL
Match: A0A0A0L9V6 (Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa3G698490 PE=2 SV=1)

HSP 1 Score: 1041 bits (2692), Expect = 0.0
Identity = 522/524 (99.62%), Postives = 523/524 (99.81%), Query Frame = 0

Query: 1   MPDAALHYTLYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLLPATH 60
           MPDAALHYTLYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLLPATH
Sbjct: 1   MPDAALHYTLYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLLPATH 60

Query: 61  KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV 120
           KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV
Sbjct: 61  KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV 120

Query: 121 GFLGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGA 180
           GFLGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGA
Sbjct: 121 GFLGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGA 180

Query: 181 ELIKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFW 240
           ELIKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFT+GPLKRLPFW
Sbjct: 181 ELIKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTRGPLKRLPFW 240

Query: 241 LFGNKALQINVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRN 300
           LFGNKALQINVRFDLLLENILQQHEQRAKIHGLERED DLMDILLKAYLDEKAEFKMTRN
Sbjct: 241 LFGNKALQINVRFDLLLENILQQHEQRAKIHGLEREDRDLMDILLKAYLDEKAEFKMTRN 300

Query: 301 HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGTRLVEETDVTNLP 360
           HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGTRLVEETDVTNLP
Sbjct: 301 HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGTRLVEETDVTNLP 360

Query: 361 YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF 420
           YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF
Sbjct: 361 YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF 420

Query: 421 RPERFYNENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQC 480
           RPERFYNENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQC
Sbjct: 421 RPERFYNENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQC 480

Query: 481 FDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVAQ 524
           FDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVAQ
Sbjct: 481 FDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVAQ 524

BLAST of Cucsat.G12140.T2 vs. ExPASy TrEMBL
Match: A0A5D3CH28 (Cytochrome P450 705A5-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold25G00320 PE=3 SV=1)

HSP 1 Score: 1014 bits (2623), Expect = 0.0
Identity = 506/524 (96.56%), Postives = 512/524 (97.71%), Query Frame = 0

Query: 1   MPDAALHYTLYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLLPATH 60
           MPDAALHY LYLLLFF SSLLLFFFLHKPSKSA  NLTLPPSPPPLPVIGHLHLLLPATH
Sbjct: 1   MPDAALHYALYLLLFFFSSLLLFFFLHKPSKSAPPNLTLPPSPPPLPVIGHLHLLLPATH 60

Query: 61  KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV 120
           KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV
Sbjct: 61  KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV 120

Query: 121 GFLGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGA 180
           GFLGAPYGDYWRYMKKLTMMEVLA PQLARSRFVRNEEILRMLQKLLLCSKQK+SVDLGA
Sbjct: 121 GFLGAPYGDYWRYMKKLTMMEVLAPPQLARSRFVRNEEILRMLQKLLLCSKQKESVDLGA 180

Query: 181 ELIKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFW 240
           EL+KLTNNSICRMMMSTRCSEENNEAEKIR LVNDTIEIATKMAFGDLF QGPLKRLPFW
Sbjct: 181 ELVKLTNNSICRMMMSTRCSEENNEAEKIRTLVNDTIEIATKMAFGDLFGQGPLKRLPFW 240

Query: 241 LFGNKALQINVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRN 300
           LFG KAL+INVRFD+LLENILQQHEQRAKIHGLERED DLMDILLKAYLDEKAEFKMTRN
Sbjct: 241 LFGKKALEINVRFDVLLENILQQHEQRAKIHGLEREDRDLMDILLKAYLDEKAEFKMTRN 300

Query: 301 HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGTRLVEETDVTNLP 360
           HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESV GTRLVEETDVTNLP
Sbjct: 301 HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVVGTRLVEETDVTNLP 360

Query: 361 YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF 420
           YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKD MVAVDLFAIMRDPNLWENPDEF
Sbjct: 361 YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDTMVAVDLFAIMRDPNLWENPDEF 420

Query: 421 RPERFYNENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQC 480
           RPERFYNENSSKEEGTKHIQYEIKGQ+FSFVPFGGGRRGCPGSLLAFNTINRTVAALVQC
Sbjct: 421 RPERFYNENSSKEEGTKHIQYEIKGQNFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQC 480

Query: 481 FDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVAQ 524
           FDWKVGKDGDEEKVNMEIG+GISLPMAHPLICVPVSHSTPFVA 
Sbjct: 481 FDWKVGKDGDEEKVNMEIGSGISLPMAHPLICVPVSHSTPFVAH 524

BLAST of Cucsat.G12140.T2 vs. ExPASy TrEMBL
Match: A0A1S3CGU3 (cytochrome P450 705A5-like OS=Cucumis melo OX=3656 GN=LOC103500305 PE=3 SV=1)

HSP 1 Score: 1014 bits (2623), Expect = 0.0
Identity = 506/524 (96.56%), Postives = 512/524 (97.71%), Query Frame = 0

Query: 1   MPDAALHYTLYLLLFFLSSLLLFFFLHKPSKSASANLTLPPSPPPLPVIGHLHLLLPATH 60
           MPDAALHY LYLLLFF SSLLLFFFLHKPSKSA  NLTLPPSPPPLPVIGHLHLLLPATH
Sbjct: 1   MPDAALHYALYLLLFFFSSLLLFFFLHKPSKSAPPNLTLPPSPPPLPVIGHLHLLLPATH 60

Query: 61  KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV 120
           KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV
Sbjct: 61  KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV 120

Query: 121 GFLGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGA 180
           GFLGAPYGDYWRYMKKLTMMEVLA PQLARSRFVRNEEILRMLQKLLLCSKQK+SVDLGA
Sbjct: 121 GFLGAPYGDYWRYMKKLTMMEVLAPPQLARSRFVRNEEILRMLQKLLLCSKQKESVDLGA 180

Query: 181 ELIKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFW 240
           EL+KLTNNSICRMMMSTRCSEENNEAEKIR LVNDTIEIATKMAFGDLF QGPLKRLPFW
Sbjct: 181 ELVKLTNNSICRMMMSTRCSEENNEAEKIRTLVNDTIEIATKMAFGDLFGQGPLKRLPFW 240

Query: 241 LFGNKALQINVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRN 300
           LFG KAL+INVRFD+LLENILQQHEQRAKIHGLERED DLMDILLKAYLDEKAEFKMTRN
Sbjct: 241 LFGKKALEINVRFDVLLENILQQHEQRAKIHGLEREDRDLMDILLKAYLDEKAEFKMTRN 300

Query: 301 HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGTRLVEETDVTNLP 360
           HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESV GTRLVEETDVTNLP
Sbjct: 301 HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVVGTRLVEETDVTNLP 360

Query: 361 YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF 420
           YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKD MVAVDLFAIMRDPNLWENPDEF
Sbjct: 361 YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDTMVAVDLFAIMRDPNLWENPDEF 420

Query: 421 RPERFYNENSSKEEGTKHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQC 480
           RPERFYNENSSKEEGTKHIQYEIKGQ+FSFVPFGGGRRGCPGSLLAFNTINRTVAALVQC
Sbjct: 421 RPERFYNENSSKEEGTKHIQYEIKGQNFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQC 480

Query: 481 FDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVAQ 524
           FDWKVGKDGDEEKVNMEIG+GISLPMAHPLICVPVSHSTPFVA 
Sbjct: 481 FDWKVGKDGDEEKVNMEIGSGISLPMAHPLICVPVSHSTPFVAH 524

BLAST of Cucsat.G12140.T2 vs. ExPASy TrEMBL
Match: A0A6J1GBR6 (cytochrome P450 705A22-like OS=Cucurbita moschata OX=3662 GN=LOC111452721 PE=3 SV=1)

HSP 1 Score: 903 bits (2334), Expect = 0.0
Identity = 453/526 (86.12%), Postives = 484/526 (92.02%), Query Frame = 0

Query: 8   YTLYLLLFFLSSLLLFFFLHKPSKSAS-------ANLTLPPSPPPLPVIGHLHLLLPATH 67
           Y LY LLFFLS L L FFLHK S SAS       A LTLPPSPP LP+IGHLHLLLPA +
Sbjct: 4   YILYFLLFFLSCLFLLFFLHKTSGSASSSSSAAAAKLTLPPSPPSLPLIGHLHLLLPANY 63

Query: 68  KSFFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKV 127
           KSFFNISSKYGPLLHIRL +LQYVLVSSASLAAEIF+THDLTFSSRPDFAFSEEYPYGKV
Sbjct: 64  KSFFNISSKYGPLLHIRLCSLQYVLVSSASLAAEIFRTHDLTFSSRPDFAFSEEYPYGKV 123

Query: 128 GFLGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGA 187
           GFLGAPYGDYWRYMKKLTMMEVLA PQLARSRFVRNEEI RML KLLL S++K+SVDLGA
Sbjct: 124 GFLGAPYGDYWRYMKKLTMMEVLAPPQLARSRFVRNEEISRMLHKLLLSSQKKESVDLGA 183

Query: 188 ELIKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFW 247
           EL+KLTNNSICRMMMSTRCSEENNEAE IR LVNDTIEIATK+AFGDLF++GPLKRLPFW
Sbjct: 184 ELVKLTNNSICRMMMSTRCSEENNEAENIRKLVNDTIEIATKLAFGDLFSEGPLKRLPFW 243

Query: 248 LFGNKALQINVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRN 307
           LFG K L++N+RFD+LLE ILQQHE+RAKIHG ER+D DLMDILLKAYLDEKAEFK+TRN
Sbjct: 244 LFGKKVLKLNIRFDVLLEKILQQHEERAKIHGFERDDRDLMDILLKAYLDEKAEFKITRN 303

Query: 308 HIKAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGTRLVEETDVTNLP 367
           HIKAFLLDLFIAGTGTSAEVMQW +AEL+NHP+VF+KVR+EIE V G+RLVEETD+ NLP
Sbjct: 304 HIKAFLLDLFIAGTGTSAEVMQWTIAELINHPNVFRKVRQEIEYVVGSRLVEETDIVNLP 363

Query: 368 YTQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF 427
           Y QAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF
Sbjct: 364 YMQAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEF 423

Query: 428 RPERFYNENSSKEEGT---KHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAAL 487
           RPERFYN N++K+EG    KHIQYEIKGQ+FSFVPFGGGRRGCPGSLLA+NTINRTVAAL
Sbjct: 424 RPERFYNANTNKDEGDQGMKHIQYEIKGQNFSFVPFGGGRRGCPGSLLAYNTINRTVAAL 483

Query: 488 VQCFDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVA 523
           VQCFDWKVGKDG+ EKVNMEIGTGISLPMAH  ICVPVSHSTPFVA
Sbjct: 484 VQCFDWKVGKDGNGEKVNMEIGTGISLPMAHAFICVPVSHSTPFVA 529

BLAST of Cucsat.G12140.T2 vs. ExPASy TrEMBL
Match: A0A6J1KA72 (cytochrome P450 705A22-like OS=Cucurbita maxima OX=3661 GN=LOC111493061 PE=3 SV=1)

HSP 1 Score: 902 bits (2330), Expect = 0.0
Identity = 453/524 (86.45%), Postives = 482/524 (91.98%), Query Frame = 0

Query: 8   YTLYLLLFFLSSLLLFFFLHKPSKSAS-----ANLTLPPSPPPLPVIGHLHLLLPATHKS 67
           Y LY LLFFLS L L F LHK S SAS     A LTLPPSPP LP+IGHLHLLLPA +KS
Sbjct: 4   YILYFLLFFLSCLFLLFLLHKTSGSASSSAAAAKLTLPPSPPSLPLIGHLHLLLPANYKS 63

Query: 68  FFNISSKYGPLLHIRLGALQYVLVSSASLAAEIFKTHDLTFSSRPDFAFSEEYPYGKVGF 127
           FFNISSKYGPLLHIRL +LQYVLVSSASLAAEIF+THDLTFSSRPDFAFSEEYPYGKVGF
Sbjct: 64  FFNISSKYGPLLHIRLCSLQYVLVSSASLAAEIFRTHDLTFSSRPDFAFSEEYPYGKVGF 123

Query: 128 LGAPYGDYWRYMKKLTMMEVLAAPQLARSRFVRNEEILRMLQKLLLCSKQKQSVDLGAEL 187
           LGAPYGDYWRYMKKLTMMEVLA PQLARSRFVRNEEI RML KLLL S++K+SVDLGAEL
Sbjct: 124 LGAPYGDYWRYMKKLTMMEVLAPPQLARSRFVRNEEISRMLHKLLLSSQKKESVDLGAEL 183

Query: 188 IKLTNNSICRMMMSTRCSEENNEAEKIRILVNDTIEIATKMAFGDLFTQGPLKRLPFWLF 247
           IKLTNNSICRMMMSTRCSEENNEAE IR LVNDTIEIATK+AFGDLF++GPLKRLPFWLF
Sbjct: 184 IKLTNNSICRMMMSTRCSEENNEAENIRKLVNDTIEIATKLAFGDLFSEGPLKRLPFWLF 243

Query: 248 GNKALQINVRFDLLLENILQQHEQRAKIHGLEREDCDLMDILLKAYLDEKAEFKMTRNHI 307
           G K L++N+RFD+LLE ILQQHE+RAKIHG ER+D DLMDILLKAYLDEKAEFK+TRNHI
Sbjct: 244 GKKVLKLNIRFDVLLEKILQQHEERAKIHGFERDDRDLMDILLKAYLDEKAEFKITRNHI 303

Query: 308 KAFLLDLFIAGTGTSAEVMQWAMAELMNHPDVFQKVRREIESVAGTRLVEETDVTNLPYT 367
           KAFLLDLFIAGTGTSAEVMQW +AEL+NHP+VF+KVR+EIE V G+RLVEETD+ NLPY 
Sbjct: 304 KAFLLDLFIAGTGTSAEVMQWTIAELINHPNVFRKVRQEIECVVGSRLVEETDIVNLPYM 363

Query: 368 QAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEFRP 427
           QAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEFRP
Sbjct: 364 QAVVKECLRLYPAVPVARRACRETCKVNGYDIPKDIMVAVDLFAIMRDPNLWENPDEFRP 423

Query: 428 ERFYNENSSKEEGT---KHIQYEIKGQSFSFVPFGGGRRGCPGSLLAFNTINRTVAALVQ 487
           ERFYN N +K+EG    KHIQYEIKGQ+FSFVPFGGGRRGCPGSLLA+NTINRTVAALVQ
Sbjct: 424 ERFYNANPNKDEGDQGMKHIQYEIKGQNFSFVPFGGGRRGCPGSLLAYNTINRTVAALVQ 483

Query: 488 CFDWKVGKDGDEEKVNMEIGTGISLPMAHPLICVPVSHSTPFVA 523
           CFDWKVGKDG+ EKVNMEIGTGISLPMAH  ICVPVSHSTPFVA
Sbjct: 484 CFDWKVGKDGNGEKVNMEIGTGISLPMAHSFICVPVSHSTPFVA 527

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LJY51.7e-12044.55Cytochrome P450 705A22 OS=Arabidopsis thaliana OX=3702 GN=CYP705A22 PE=2 SV=1[more]
Q9FI392.1e-11846.46Cytochrome P450 705A5 OS=Arabidopsis thaliana OX=3702 GN=CYP705A5 PE=2 SV=1[more]
O819732.1e-11044.38Cytochrome P450 93A3 OS=Glycine max OX=3847 GN=CYP93A3 PE=2 SV=1[more]
Q9LJY71.0e-10944.02Cytochrome P450 705A20 OS=Arabidopsis thaliana OX=3702 GN=CYP705A20 PE=2 SV=1[more]
Q9FH674.8e-10742.75Cytochrome P450 705A12 OS=Arabidopsis thaliana OX=3702 GN=CYP705A12 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
NP_001292616.10.099.62cytochrome P450 705A5-like [Cucumis sativus] >AIT72034.1 cytochrome P450 [Cucumi... [more]
XP_008461786.10.096.56PREDICTED: cytochrome P450 705A5-like [Cucumis melo] >KAA0048327.1 cytochrome P4... [more]
XP_038903604.10.088.80cytochrome P450 705A22-like [Benincasa hispida][more]
XP_022949336.10.086.12cytochrome P450 705A22-like [Cucurbita moschata][more]
XP_022998426.10.086.45cytochrome P450 705A22-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A0A0L9V60.099.62Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa3G698490 PE=2 SV=1[more]
A0A5D3CH280.096.56Cytochrome P450 705A5-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3CGU30.096.56cytochrome P450 705A5-like OS=Cucumis melo OX=3656 GN=LOC103500305 PE=3 SV=1[more]
A0A6J1GBR60.086.12cytochrome P450 705A22-like OS=Cucurbita moschata OX=3662 GN=LOC111452721 PE=3 S... [more]
A0A6J1KA720.086.45cytochrome P450 705A22-like OS=Cucurbita maxima OX=3661 GN=LOC111493061 PE=3 SV=... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 91..112
score: 23.95
coord: 67..86
score: 31.3
coord: 450..460
score: 54.96
coord: 460..483
score: 29.12
coord: 301..318
score: 37.76
coord: 321..347
score: 35.12
coord: 185..203
score: 21.97
coord: 363..381
score: 32.84
IPR001128Cytochrome P450PRINTSPR00385P450coord: 312..329
score: 30.8
coord: 451..460
score: 56.5
coord: 364..375
score: 47.23
IPR001128Cytochrome P450PFAMPF00067p450coord: 40..504
e-value: 3.5E-90
score: 303.0
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 33..513
e-value: 3.7E-114
score: 383.9
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 36..514
NoneNo IPR availablePIRSRPIRSR000209-1PIRSR000209-1coord: 41..484
e-value: 2.2E-33
score: 112.8
NoneNo IPR availablePANTHERPTHR24298:SF59CYTOCHROME P450, FAMILY 705, SUBFAMILY A, POLYPEPTIDE 25-RELATEDcoord: 7..521
NoneNo IPR availablePANTHERPTHR24298FLAVONOID 3'-MONOOXYGENASE-RELATEDcoord: 7..521
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 453..462

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G12140Cucsat.G12140gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G12140.T2.E1Cucsat.G12140.T2.E1exon
Cucsat.G12140.T2.E2Cucsat.G12140.T2.E2exon
Cucsat.G12140.T2.E3Cucsat.G12140.T2.E3exon
Cucsat.G12140.T2.E4Cucsat.G12140.T2.E4exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G12140.T2.C3Cucsat.G12140.T2.C3CDS
Cucsat.G12140.T2.C2Cucsat.G12140.T2.C2CDS
Cucsat.G12140.T2.C1Cucsat.G12140.T2.C1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G12140.T2Cucsat.G12140.T2-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen