Cucsat.G12111.T5 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G12111.T5
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	GDSL esterase/lipase
Location	ctg1837: 519228 .. 521806 (-)
RNA-Seq Expression	Cucsat.G12111.T5
Synteny	Cucsat.G12111.T5

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TCACTTTTGTCTGATGTCAGGATTTGCAGTTTCTCTCCACCATTCTTTACAAGTTAAAGCAACTTACCACCAACTTGCATCAGAACCGGTGAAAATTCTCCAAATTGGACAAAAATTGTAGTTTCTTGGAAAGTAATGCCGATACTCTCCCTCTTTAAAGGTTTGTGTAGGAAGAGTTACATGAATCTTGCCATTTTTCCTTCTGGTAAGTGGTAGTAAGTTGGATGGATAAAAGTGGAGGCTGGTCATAAACCTTTTTTTCTTCTTATTGGGCCCAAACGACTTTAATCTTATTAATCGTGGAAGACATTAATCGATCATATCAGATTTTTTTAGAAATTTTAATTTGGCTTCTTGTGAATTCAATACGCTTTGAGTGCTTAACTAGCTGAGACCCAAGGAAATCTCAAAGAGAGCACATGGAGTGCAACTGGGATCTTTAGTGCTCACTACACATGACATTGTCTCTCTGCTCTTTATTAATTTGTGATCATTAGTTACCATAAACAAGAGGGTTAATGAATCTGATTACAGAAATGGAGGAAGCGAAGAGGGATTCCTCCCCTTTTCCATTGTTTCTGATCTTCTTCCTACTTTCTCTACCTGATCCTCTTTTTGTTCATTCCCAGTGCATCCAAAAGCCAGTTATTTTCAACTTTGGTGACTCGAATTCTGATACTGGTGGTTTTTCTGAGGGACTTGGCATCAAATTCGGACTCCCAACTGGACGCACATTCTTCCATAAACCAGCAGGAAGATTATGCGATGGACGTTTAATGATTGATTTTCTATGTACGTACTCTGATCCAACAAAACAAGTGCTCAATAGTTTGATTTTCTTACTAAAATGCAAATTCTTCCTCAGGTGAAAGTGTGAATTCTGATTATTTGACCCCATATCTGCGATCTGTGGGCCCAAATTTCACCAATGGAGCCAACTTTGCTATATCTGGTTCAGCTACTCTGCCAAAGGATCGTCCATTTAATTTGTATATTCAAATTATGCAATTCCTTCAATTTCAATCACGCTCCCTCGAGCTTATTCCAAAAGGTAGTAGTTGATTAAACACACACGGCACATCCTTAATAGATAGGAAGCATCTCTTCATTCTCCTATTGACTAAGGTTTCTAAGCCAATTGAACTGATGTAGGTTATAAAGACTTGGTTGATGAAGAAGGGTTCAATAATGCACTTTACACAATTGACATCGGACAAAATGATCTTGCTGCTGCATTCACCTACTTGTCTTATTCACAAGTCATTCAGCAAATTCCCTCTTTTGTTTCTGAGATAAAAAATGCCATATGGGTTAGCCTTCAATTTCTACACTATTTACCACCTAAAAGTTTAAGCTAAATGAGTCGTGAGAAATCTTTTGATTTATTTTCAATACTCCACCTTATGTGTTAGACTCAAATATTAGCAGATATAAAATCACCTTATGTGTTAGACTCAAATATTAGTAGATATAAAATCACCATATGTGTTAGACTCAAATATTAGTAGATATAAAATCACCTTATGTGTAGACTCAAATATTAGCAGATATAAAATCACTTTATGTGTTAGACTCAAATATTAGTATGTAGAAAATATGATAGAAATGACAGTATAAAAGTCTGAAGTGAGACCTGAAGTTTAGTTAATTCTCCATTTTTAGAAAATTCTCAATCTACACATCTAATATCAGAAAGTCGAAGGGCATGTTGTAAATATTTCTTCTGGCCTTAAAAGTAGAAGTTAAAAAGAAAACTGTCTAGATTCTTATGTTTTTGCTTCTTTCCCTTAATTGCTTTTCAATCCATACGTAGACCATATACCAACATGGAGGAAGAAATTTTTGGATACACAACACAGGGCCATTGGGCTGTTTACCTCAAAAGCTCGCTACAGCTTATGCAGATGCAAATGCCAGTGACATTGACAATCATGGATGCCTCCAATCCTTCAACAACGCTGCAAAAGAGTTCAACACTCAGCTAAGAGTTGCATGTGGAGAACTAAGATCTGCTTTGACAAATGCCACCATCGTGTATGTAGACGTCTACGCCATCAAATATGACCTCATAACCAACTCTGTCTCCAACGGTATTGCCAATTATATAATTATCATCATCTTCTTCTAACAAGAATCAGCAACATGATAATCTTGACATCTACATTGTGCAGGGTTTGAGAATCCATTGATTGTGTGTTGTGGCTATGGAGGACCACCATACAACTTCAACCAGACCGTTACTTGCGGCCAGCCGGGATTCAACACGTGCAATGAAGGATTGAAGTATGTAAGTTGGGACGGAGTTCACTATACAGAAGCAGCAAATGCTGTGTTTGCCTCCATGATACTTTCTTCTCAATACTCTTCTCCAAAACTTCCCTTTAATTTCTTCTGTAACAAGTGATGATTGGAGTCATTATGGCCCAATATAAAAAGTTCATATAATTTGGATAATGTTTAGTGATTGGACTTTTGTTGGACGTTTGCTCTTTTTACTGTTTTATAGAGAGTATTGGCAAAACTGTAATCAATGAAAAGCAAAGGATGCTATACAAAAACTTATTAGTAATGAAAATCAT

Coding sequence (CDS)

ATGAATCTGATTACAGAAATGGAGGAAGCGAAGAGGGATTCCTCCCCTTTTCCATTGTTTCTGATCTTCTTCCTACTTTCTCTACCTGATCCTCTTTTTGTTCATTCCCAGTGCATCCAAAAGCCAGTTATTTTCAACTTTGGTGACTCGAATTCTGATACTGGTGGTTTTTCTGAGGGACTTGGCATCAAATTCGGACTCCCAACTGGACGCACATTCTTCCATAAACCAGCAGGAAGATTATGCGATGGACGTTTAATGATTGATTTTCTATGTGAAAGTGTGAATTCTGATTATTTGACCCCATATCTGCGATCTGTGGGCCCAAATTTCACCAATGGAGCCAACTTTGCTATATCTGGTTCAGCTACTCTGCCAAAGGATCGTCCATTTAATTTGTATATTCAAATTATGCAATTCCTTCAATTTCAATCACGCTCCCTCGAGCTTATTCCAAAAGGTAGTAGTTGA

Protein sequence

MNLITEMEEAKRDSSPFPLFLIFFLLSLPDPLFVHSQCIQKPVIFNFGDSNSDTGGFSEGLGIKFGLPTGRTFFHKPAGRLCDGRLMIDFLCESVNSDYLTPYLRSVGPNFTNGANFAISGSATLPKDRPFNLYIQIMQFLQFQSRSLELIPKGSS

Homology

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BLAST of Cucsat.G12111.T5 vs. ExPASy Swiss-Prot
Match: O80522 (GDSL esterase/lipase At1g09390 OS=Arabidopsis thaliana OX=3702 GN=At1g09390 PE=2 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 3.2e-38
Identity = 84/138 (60.87%), Postives = 97/138 (70.29%), Query Frame = 0


Query: 14  SSPFPLFLIFFLLSLPDPLFVHSQCIQKPVIFNFGDSNSDTGGFSEGLGIKFGLPTGRTF 73
           S  F L L+ F+L L   L V   C   PVIFNFGDSNSDTGG   GLG   GLP GR+F
Sbjct: 8   SHSFLLVLLPFILILRQNLAVAGGCQVPPVIFNFGDSNSDTGGLVAGLGYSIGLPNGRSF 67

Query: 74  FHKPAGRLCDGRLMIDFLCESVNSDYLTPYLRS-VGPNFTNGANFAISGSATLPKDRPFN 133
           F +  GRL DGRL+IDFLC+S+N+  L PYL S VG  F NGANFAI GS+TLP+  PF 
Sbjct: 68  FQRSTGRLSDGRLVIDFLCQSLNTSLLNPYLDSLVGSKFQNGANFAIVGSSTLPRYVPFA 127

Query: 134 LYIQIMQFLQFQSRSLEL 151
           L IQ+MQFL F+SR+LEL
Sbjct: 128 LNIQLMQFLHFKSRALEL 145

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BLAST of Cucsat.G12111.T5 vs. ExPASy Swiss-Prot
Match: Q9FXB6 (GDSL esterase/lipase LIP-4 OS=Arabidopsis thaliana OX=3702 GN=LIP4 PE=2 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 3.0e-36
Identity = 83/148 (56.08%), Postives = 97/148 (65.54%), Query Frame = 0


Query: 14  SSPFPLFLI----FFLLSLPDPLFVHSQCIQKPVIFNFGDSNSDTGGFSEGLGIKFGLPT 73
           S+ F  F I      LL L  P    S C  +PVIFNFGDSNSDTGG   GLG   G P 
Sbjct: 8   SNTFSFFFITLVSLALLILRQPSRAAS-CTARPVIFNFGDSNSDTGGLVAGLGYPIGFPN 67

Query: 74  GRTFFHKPAGRLCDGRLMIDFLCESVNSDYLTPYLRSVG-PNFTNGANFAISGSATLPKD 133
           GR FF +  GRL DGRL+IDFLC+S+N+  L PYL S+G   F NGANFAI+GS TLPK+
Sbjct: 68  GRLFFRRSTGRLSDGRLLIDFLCQSLNTSLLRPYLDSLGRTRFQNGANFAIAGSPTLPKN 127

Query: 134 RPFNLYIQIMQFLQFQSRSLELIPKGSS 157
            PF+L IQ+ QF  F+SRSLEL    +S
Sbjct: 128 VPFSLNIQVKQFSHFKSRSLELASSSNS 154

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BLAST of Cucsat.G12111.T5 vs. ExPASy Swiss-Prot
Match: Q6NLP7 (GDSL esterase/lipase At3g62280 OS=Arabidopsis thaliana OX=3702 GN=At3g62280 PE=2 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 2.2e-31
Identity = 65/115 (56.52%), Postives = 81/115 (70.43%), Query Frame = 0


Query: 40  QKPVIFNFGDSNSDTGGFSEGLGIKFGLPTGRTFFHKPAGRLCDGRLMIDFLCESVNSDY 99
           +KP++ NFGDSNSDTGG   G+G+  GLP G TFFH+  GRL DGRL++DF CE +   Y
Sbjct: 33  KKPILINFGDSNSDTGGVLAGVGLPIGLPHGITFFHRGTGRLGDGRLIVDFYCEHLKMTY 92

Query: 100 LTPYLRSVGPNFTNGANFAISGSATLPKDRPFNLYIQIMQFLQFQSRSLELIPKG 155
           L+PYL S+ PNF  G NFA+SG+  LP    F L IQI QF+ F++RS ELI  G
Sbjct: 93  LSPYLDSLSPNFKRGVNFAVSGATALP-IFSFPLAIQIRQFVHFKNRSQELISSG 146

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BLAST of Cucsat.G12111.T5 vs. ExPASy Swiss-Prot
Match: Q3ECP6 (GDSL esterase/lipase At1g54790 OS=Arabidopsis thaliana OX=3702 GN=At1g54790 PE=2 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.2e-26
Identity = 64/138 (46.38%), Postives = 87/138 (63.04%), Query Frame = 0


Query: 21  LIFFLLSLPDPLFVHSQCIQKPVIFNFGDSNSDTGGFSEGLGIKFGLPTGRTFFHKPAGR 80
           ++FF+ SL      +S     P  FNFGDSNSDTG    GLGI+  LP G+  F   + R
Sbjct: 12  ILFFISSLQ---ISNSIDFNYPSAFNFGDSNSDTGDLVAGLGIRLDLPNGQNSFKTSSQR 71

Query: 81  LCDGRLMIDFLCESVNSDYLTPYLRSVG-PNFTNGANFAISGSATLPKD----RPFNLYI 140
            CDGRL+IDFL + ++  +L PYL S+G PNF  G NFA +GS  LP +     PF+  +
Sbjct: 72  FCDGRLVIDFLMDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFSFDL 131

Query: 141 QIMQFLQFQSRSLELIPK 154
           QI QF++F+SR++EL+ K
Sbjct: 132 QISQFIRFKSRAIELLSK 146

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BLAST of Cucsat.G12111.T5 vs. ExPASy Swiss-Prot
Match: Q9FXE5 (Alpha-L-fucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=FXG1 PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 2.1e-26
Identity = 69/147 (46.94%), Postives = 89/147 (60.54%), Query Frame = 0


Query: 18  PLFLIFFLLSLPDPL--FVHSQCIQKPVIFNFGDSNSDTGGFSEGLGIKFGLPTGRTFFH 77
           P+    F LSL   L    H+     P IFNFGDSNSDTGG S   G + G P G +FF 
Sbjct: 3   PILSSLFALSLLSSLSPSTHAHQCHFPAIFNFGDSNSDTGGLSAAFG-QAGPPHGSSFFG 62

Query: 78  KPAGRLCDGRLMIDFLCESVNSDYLTPYLRSVGPNFTNGANFAISGS------ATLPKD- 137
            PAGR CDGRL+IDF+ ES+   YL+ +L SVG NF++GANFA +GS      +TL +  
Sbjct: 63  SPAGRYCDGRLVIDFIAESLGLPYLSAFLDSVGSNFSHGANFATAGSPIRALNSTLRQSG 122

Query: 138 -RPFNLYIQIMQFLQFQSRSLELIPKG 155
             PF+L +Q +QF  F +RS  +  +G
Sbjct: 123 FSPFSLDVQFVQFYNFHNRSQTVRSRG 148

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BLAST of Cucsat.G12111.T5 vs. NCBI nr
Match: XP_004150835.1 (GDSL esterase/lipase At1g09390 [Cucumis sativus] >KGN58540.1 hypothetical protein Csa_001237 [Cucumis sativus])

HSP 1 Score: 318 bits (815), Expect = 2.32e-106
Identity = 153/154 (99.35%), Postives = 153/154 (99.35%), Query Frame = 0


Query: 1   MNLITEMEEAKRDSSPFPLFLIFFLLSLPDPLFVHSQCIQKPVIFNFGDSNSDTGGFSEG 60
           MNLITEMEE KRDSSPFPLFLIFFLLSLPDPLFVHSQCIQKPVIFNFGDSNSDTGGFSEG
Sbjct: 1   MNLITEMEEEKRDSSPFPLFLIFFLLSLPDPLFVHSQCIQKPVIFNFGDSNSDTGGFSEG 60

Query: 61  LGIKFGLPTGRTFFHKPAGRLCDGRLMIDFLCESVNSDYLTPYLRSVGPNFTNGANFAIS 120
           LGIKFGLPTGRTFFHKPAGRLCDGRLMIDFLCESVNSDYLTPYLRSVGPNFTNGANFAIS
Sbjct: 61  LGIKFGLPTGRTFFHKPAGRLCDGRLMIDFLCESVNSDYLTPYLRSVGPNFTNGANFAIS 120

Query: 121 GSATLPKDRPFNLYIQIMQFLQFQSRSLELIPKG 154
           GSATLPKDRPFNLYIQIMQFLQFQSRSLELIPKG
Sbjct: 121 GSATLPKDRPFNLYIQIMQFLQFQSRSLELIPKG 154

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BLAST of Cucsat.G12111.T5 vs. NCBI nr
Match: XP_008447456.1 (PREDICTED: GDSL esterase/lipase At1g09390-like [Cucumis melo])

HSP 1 Score: 305 bits (780), Expect = 4.89e-101
Identity = 146/154 (94.81%), Postives = 149/154 (96.75%), Query Frame = 0


Query: 1   MNLITEMEEAKRDSSPFPLFLIFFLLSLPDPLFVHSQCIQKPVIFNFGDSNSDTGGFSEG 60
           MNLITEMEE KR S PFPLF+IFFLLSLP PLFVHSQCIQKPVIFNFGDSNSDTGG SEG
Sbjct: 1   MNLITEMEEEKRTSFPFPLFVIFFLLSLPGPLFVHSQCIQKPVIFNFGDSNSDTGGLSEG 60

Query: 61  LGIKFGLPTGRTFFHKPAGRLCDGRLMIDFLCESVNSDYLTPYLRSVGPNFTNGANFAIS 120
           LGIKFGLPTGRTFFHKPAGRLCDGRLMIDFLCESV+SDYLTPYLRSVGPNFTNGANFAIS
Sbjct: 61  LGIKFGLPTGRTFFHKPAGRLCDGRLMIDFLCESVSSDYLTPYLRSVGPNFTNGANFAIS 120

Query: 121 GSATLPKDRPFNLYIQIMQFLQFQSRSLELIPKG 154
           GSATLPKDRPFNLY+QIMQFLQFQSRSLELIPKG
Sbjct: 121 GSATLPKDRPFNLYVQIMQFLQFQSRSLELIPKG 154

[Close]

BLAST of Cucsat.G12111.T5 vs. NCBI nr
Match: KAA0038109.1 (GDSL esterase/lipase [Cucumis melo var. makuwa] >TYK20498.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 293 bits (750), Expect = 1.57e-96
Identity = 140/148 (94.59%), Postives = 143/148 (96.62%), Query Frame = 0


Query: 7   MEEAKRDSSPFPLFLIFFLLSLPDPLFVHSQCIQKPVIFNFGDSNSDTGGFSEGLGIKFG 66
           MEE KR S PFPLF+IFFLLSLP PLFVHSQCIQKPVIFNFGDSNSDTGG SEGLGIKFG
Sbjct: 1   MEEEKRTSFPFPLFVIFFLLSLPGPLFVHSQCIQKPVIFNFGDSNSDTGGLSEGLGIKFG 60

Query: 67  LPTGRTFFHKPAGRLCDGRLMIDFLCESVNSDYLTPYLRSVGPNFTNGANFAISGSATLP 126
           LPTGRTFFHKPAGRLCDGRLMIDFLCESV+SDYLTPYLRSVGPNFTNGANFAISGSATLP
Sbjct: 61  LPTGRTFFHKPAGRLCDGRLMIDFLCESVSSDYLTPYLRSVGPNFTNGANFAISGSATLP 120

Query: 127 KDRPFNLYIQIMQFLQFQSRSLELIPKG 154
           KDRPFNLY+QIMQFLQFQSRSLELIPKG
Sbjct: 121 KDRPFNLYVQIMQFLQFQSRSLELIPKG 148

[Close]

BLAST of Cucsat.G12111.T5 vs. NCBI nr
Match: XP_038905016.1 (GDSL esterase/lipase At1g09390-like [Benincasa hispida])

HSP 1 Score: 292 bits (747), Expect = 4.48e-96
Identity = 139/154 (90.26%), Postives = 148/154 (96.10%), Query Frame = 0


Query: 1   MNLITEMEEAKRDSSPFPLFLIFFLLSLPDPLFVHSQCIQKPVIFNFGDSNSDTGGFSEG 60
           MNLITEM + KR+S PFPL +IFFLLSLP PLFVHSQC QKPVIFNFGDSNSDTGGFSEG
Sbjct: 1   MNLITEMAKTKRNSFPFPLPVIFFLLSLPGPLFVHSQCTQKPVIFNFGDSNSDTGGFSEG 60

Query: 61  LGIKFGLPTGRTFFHKPAGRLCDGRLMIDFLCESVNSDYLTPYLRSVGPNFTNGANFAIS 120
           LG+KFGLPTGRTFFHKP+GRLCDGRLMIDFLCE+V+SDYLTPYLRSVGPNFTNGANFAIS
Sbjct: 61  LGLKFGLPTGRTFFHKPSGRLCDGRLMIDFLCENVSSDYLTPYLRSVGPNFTNGANFAIS 120

Query: 121 GSATLPKDRPFNLYIQIMQFLQFQSRSLELIPKG 154
           GSATLPKDRPF+L+IQIMQFLQFQSRSLELIPKG
Sbjct: 121 GSATLPKDRPFSLHIQIMQFLQFQSRSLELIPKG 154

[Close]

BLAST of Cucsat.G12111.T5 vs. NCBI nr
Match: XP_022998461.1 (GDSL esterase/lipase At1g09390-like [Cucurbita maxima])

HSP 1 Score: 271 bits (692), Expect = 8.00e-88
Identity = 129/148 (87.16%), Postives = 136/148 (91.89%), Query Frame = 0


Query: 7   MEEAKRDSSPFPLFLIFFLLSLPDPLFVHSQCIQKPVIFNFGDSNSDTGGFSEGLGIKFG 66
           M E KR+S PFPLF+IFFLLSLP PL V SQC QKPVIFNFGDSNSDTGGFSEGLG++FG
Sbjct: 1   MTEKKRNSFPFPLFVIFFLLSLPGPLLVLSQCSQKPVIFNFGDSNSDTGGFSEGLGLRFG 60

Query: 67  LPTGRTFFHKPAGRLCDGRLMIDFLCESVNSDYLTPYLRSVGPNFTNGANFAISGSATLP 126
            P+GR FFHKP+GRLCDGRLMIDFLCESV SDYLTPYLRSVG NFTNG NFAISGSATLP
Sbjct: 61  PPSGRVFFHKPSGRLCDGRLMIDFLCESVGSDYLTPYLRSVGSNFTNGVNFAISGSATLP 120

Query: 127 KDRPFNLYIQIMQFLQFQSRSLELIPKG 154
           KDRPFNLYIQ+MQFLQFQSRSLELIPKG
Sbjct: 121 KDRPFNLYIQVMQFLQFQSRSLELIPKG 148

[Close]

BLAST of Cucsat.G12111.T5 vs. ExPASy TrEMBL
Match: A0A0A0L9E0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G669600 PE=3 SV=1)

HSP 1 Score: 318 bits (815), Expect = 1.12e-106
Identity = 153/154 (99.35%), Postives = 153/154 (99.35%), Query Frame = 0


Query: 1   MNLITEMEEAKRDSSPFPLFLIFFLLSLPDPLFVHSQCIQKPVIFNFGDSNSDTGGFSEG 60
           MNLITEMEE KRDSSPFPLFLIFFLLSLPDPLFVHSQCIQKPVIFNFGDSNSDTGGFSEG
Sbjct: 1   MNLITEMEEEKRDSSPFPLFLIFFLLSLPDPLFVHSQCIQKPVIFNFGDSNSDTGGFSEG 60

Query: 61  LGIKFGLPTGRTFFHKPAGRLCDGRLMIDFLCESVNSDYLTPYLRSVGPNFTNGANFAIS 120
           LGIKFGLPTGRTFFHKPAGRLCDGRLMIDFLCESVNSDYLTPYLRSVGPNFTNGANFAIS
Sbjct: 61  LGIKFGLPTGRTFFHKPAGRLCDGRLMIDFLCESVNSDYLTPYLRSVGPNFTNGANFAIS 120

Query: 121 GSATLPKDRPFNLYIQIMQFLQFQSRSLELIPKG 154
           GSATLPKDRPFNLYIQIMQFLQFQSRSLELIPKG
Sbjct: 121 GSATLPKDRPFNLYIQIMQFLQFQSRSLELIPKG 154

[Close]

BLAST of Cucsat.G12111.T5 vs. ExPASy TrEMBL
Match: A0A1S3BHH9 (GDSL esterase/lipase At1g09390-like OS=Cucumis melo OX=3656 GN=LOC103489900 PE=3 SV=1)

HSP 1 Score: 305 bits (780), Expect = 2.37e-101
Identity = 146/154 (94.81%), Postives = 149/154 (96.75%), Query Frame = 0


Query: 1   MNLITEMEEAKRDSSPFPLFLIFFLLSLPDPLFVHSQCIQKPVIFNFGDSNSDTGGFSEG 60
           MNLITEMEE KR S PFPLF+IFFLLSLP PLFVHSQCIQKPVIFNFGDSNSDTGG SEG
Sbjct: 1   MNLITEMEEEKRTSFPFPLFVIFFLLSLPGPLFVHSQCIQKPVIFNFGDSNSDTGGLSEG 60

Query: 61  LGIKFGLPTGRTFFHKPAGRLCDGRLMIDFLCESVNSDYLTPYLRSVGPNFTNGANFAIS 120
           LGIKFGLPTGRTFFHKPAGRLCDGRLMIDFLCESV+SDYLTPYLRSVGPNFTNGANFAIS
Sbjct: 61  LGIKFGLPTGRTFFHKPAGRLCDGRLMIDFLCESVSSDYLTPYLRSVGPNFTNGANFAIS 120

Query: 121 GSATLPKDRPFNLYIQIMQFLQFQSRSLELIPKG 154
           GSATLPKDRPFNLY+QIMQFLQFQSRSLELIPKG
Sbjct: 121 GSATLPKDRPFNLYVQIMQFLQFQSRSLELIPKG 154

[Close]

BLAST of Cucsat.G12111.T5 vs. ExPASy TrEMBL
Match: A0A5A7T9T8 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00720 PE=3 SV=1)

HSP 1 Score: 293 bits (750), Expect = 7.61e-97
Identity = 140/148 (94.59%), Postives = 143/148 (96.62%), Query Frame = 0


Query: 7   MEEAKRDSSPFPLFLIFFLLSLPDPLFVHSQCIQKPVIFNFGDSNSDTGGFSEGLGIKFG 66
           MEE KR S PFPLF+IFFLLSLP PLFVHSQCIQKPVIFNFGDSNSDTGG SEGLGIKFG
Sbjct: 1   MEEEKRTSFPFPLFVIFFLLSLPGPLFVHSQCIQKPVIFNFGDSNSDTGGLSEGLGIKFG 60

Query: 67  LPTGRTFFHKPAGRLCDGRLMIDFLCESVNSDYLTPYLRSVGPNFTNGANFAISGSATLP 126
           LPTGRTFFHKPAGRLCDGRLMIDFLCESV+SDYLTPYLRSVGPNFTNGANFAISGSATLP
Sbjct: 61  LPTGRTFFHKPAGRLCDGRLMIDFLCESVSSDYLTPYLRSVGPNFTNGANFAISGSATLP 120

Query: 127 KDRPFNLYIQIMQFLQFQSRSLELIPKG 154
           KDRPFNLY+QIMQFLQFQSRSLELIPKG
Sbjct: 121 KDRPFNLYVQIMQFLQFQSRSLELIPKG 148

[Close]

BLAST of Cucsat.G12111.T5 vs. ExPASy TrEMBL
Match: A0A6J1KA99 (GDSL esterase/lipase At1g09390-like OS=Cucurbita maxima OX=3661 GN=LOC111493087 PE=3 SV=1)

HSP 1 Score: 271 bits (692), Expect = 3.87e-88
Identity = 129/148 (87.16%), Postives = 136/148 (91.89%), Query Frame = 0


Query: 7   MEEAKRDSSPFPLFLIFFLLSLPDPLFVHSQCIQKPVIFNFGDSNSDTGGFSEGLGIKFG 66
           M E KR+S PFPLF+IFFLLSLP PL V SQC QKPVIFNFGDSNSDTGGFSEGLG++FG
Sbjct: 1   MTEKKRNSFPFPLFVIFFLLSLPGPLLVLSQCSQKPVIFNFGDSNSDTGGFSEGLGLRFG 60

Query: 67  LPTGRTFFHKPAGRLCDGRLMIDFLCESVNSDYLTPYLRSVGPNFTNGANFAISGSATLP 126
            P+GR FFHKP+GRLCDGRLMIDFLCESV SDYLTPYLRSVG NFTNG NFAISGSATLP
Sbjct: 61  PPSGRVFFHKPSGRLCDGRLMIDFLCESVGSDYLTPYLRSVGSNFTNGVNFAISGSATLP 120

Query: 127 KDRPFNLYIQIMQFLQFQSRSLELIPKG 154
           KDRPFNLYIQ+MQFLQFQSRSLELIPKG
Sbjct: 121 KDRPFNLYIQVMQFLQFQSRSLELIPKG 148

[Close]

BLAST of Cucsat.G12111.T5 vs. ExPASy TrEMBL
Match: A0A6J1DSA1 (GDSL esterase/lipase At1g09390-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111023837 PE=3 SV=1)

HSP 1 Score: 261 bits (668), Expect = 1.77e-84
Identity = 124/154 (80.52%), Postives = 138/154 (89.61%), Query Frame = 0


Query: 1   MNLITEMEEAKRDSSPFPLFLIFFLLSLPDPLFVHSQCIQKPVIFNFGDSNSDTGGFSEG 60
           MNLITEM E  R S PF LF+IFFLLSLPDPL VHSQC +KPVIFNFGDSNSDTGG+SEG
Sbjct: 1   MNLITEMAEKNRKSFPFSLFVIFFLLSLPDPLRVHSQCSRKPVIFNFGDSNSDTGGYSEG 60

Query: 61  LGIKFGLPTGRTFFHKPAGRLCDGRLMIDFLCESVNSDYLTPYLRSVGPNFTNGANFAIS 120
           LG++FG P G TFFHKP+GRLCDGRLMIDFLCESV+SDYLTPYLRSVG NFT+GANFAIS
Sbjct: 61  LGLRFGPPNGXTFFHKPSGRLCDGRLMIDFLCESVSSDYLTPYLRSVGSNFTDGANFAIS 120

Query: 121 GSATLPKDRPFNLYIQIMQFLQFQSRSLELIPKG 154
           GS+TLP++ PFNL +Q++QFLQFQS SLELI KG
Sbjct: 121 GSSTLPRNHPFNLNVQVLQFLQFQSCSLELISKG 154

The following BLAST results are available for this feature:

BLAST of Cucsat.G12111.T5 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O80522	3.2e-38	60.87	GDSL esterase/lipase At1g09390 OS=Arabidopsis thaliana OX=3702 GN=At1g09390 PE=2...	[more]
Q9FXB6	3.0e-36	56.08	GDSL esterase/lipase LIP-4 OS=Arabidopsis thaliana OX=3702 GN=LIP4 PE=2 SV=1	[more]
Q6NLP7	2.2e-31	56.52	GDSL esterase/lipase At3g62280 OS=Arabidopsis thaliana OX=3702 GN=At3g62280 PE=2...	[more]
Q3ECP6	1.2e-26	46.38	GDSL esterase/lipase At1g54790 OS=Arabidopsis thaliana OX=3702 GN=At1g54790 PE=2...	[more]
Q9FXE5	2.1e-26	46.94	Alpha-L-fucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=FXG1 PE=2 SV=1	[more]

BLAST of Cucsat.G12111.T5 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004150835.1	2.32e-106	99.35	GDSL esterase/lipase At1g09390 [Cucumis sativus] >KGN58540.1 hypothetical protei...	[more]
XP_008447456.1	4.89e-101	94.81	PREDICTED: GDSL esterase/lipase At1g09390-like [Cucumis melo]	[more]
KAA0038109.1	1.57e-96	94.59	GDSL esterase/lipase [Cucumis melo var. makuwa] >TYK20498.1 GDSL esterase/lipase...	[more]
XP_038905016.1	4.48e-96	90.26	GDSL esterase/lipase At1g09390-like [Benincasa hispida]	[more]
XP_022998461.1	8.00e-88	87.16	GDSL esterase/lipase At1g09390-like [Cucurbita maxima]	[more]

BLAST of Cucsat.G12111.T5 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L9E0	1.12e-106	99.35	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G669600 PE=3 SV=1	[more]
A0A1S3BHH9	2.37e-101	94.81	GDSL esterase/lipase At1g09390-like OS=Cucumis melo OX=3656 GN=LOC103489900 PE=3...	[more]
A0A5A7T9T8	7.61e-97	94.59	GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237...	[more]
A0A6J1KA99	3.87e-88	87.16	GDSL esterase/lipase At1g09390-like OS=Cucurbita maxima OX=3661 GN=LOC111493087 ...	[more]
A0A6J1DSA1	1.77e-84	80.52	GDSL esterase/lipase At1g09390-like isoform X1 OS=Momordica charantia OX=3673 GN...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001087	GDSL lipase/esterase	PFAM	PF00657	Lipase_GDSL	coord: 44..140 e-value: 1.4E-10 score: 42.1
IPR036514	SGNH hydrolase superfamily	GENE3D	3.40.50.1110	SGNH hydrolase	coord: 38..153 e-value: 6.5E-16 score: 60.6
None	No IPR available	PANTHER	PTHR22835	ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN	coord: 20..150
None	No IPR available	PANTHER	PTHR22835:SF158	OS01G0331100 PROTEIN	coord: 20..150

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G12111	Cucsat.G12111	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G12111.T5.E1	Cucsat.G12111.T5.E1	exon
Cucsat.G12111.T5.E2	Cucsat.G12111.T5.E2	exon
Cucsat.G12111.T5.E3	Cucsat.G12111.T5.E3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G12111.T5.C2	Cucsat.G12111.T5.C2	CDS
Cucsat.G12111.T5.C1	Cucsat.G12111.T5.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G12111.T5	Cucsat.G12111.T5-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds