Cucsat.G12022.T4 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G12022.T4
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	protein AE7-like 1
Location	ctg1820: 5276021 .. 5281601 (-)
RNA-Seq Expression	Cucsat.G12022.T4
Synteny	Cucsat.G12022.T4

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTCTCTCTTACTGGACTGTCACGCGCCTTTTCCAGCGGTGACGGATATGTTTAATGTTTAAACTAGTTTAGTATAGTTTATAGTTTATACCCACTAGTCTGTGATTAAAACTTCACCGAAACAACAAGAAGGAAGAAGAAGAAGAAGAAGGGATGACGCTGGGGCTTATCAATGCGAATCCAGTGGTTCACGCCAAGAAGGAGCGGATCGCTCGCTCGGAAGACTTCCACGGTGACGACGCTGTTGATCCTCTTGAAATCTATGATATCCTTTTCTTAACTGTTTGTGTTTTTACTAAATTTTGAGTTCTCCTTTCATTTTATTTGAACTTCAATTTTCGCTGTGGTTTCTTGCTTAATTCTGATTATCAAGTTAATTAGATTCCGTTTTGTCTGTATGCTTTTGGAGGATTCTCTTGTTTTCTTTTAGAATTTTCTAGCGCTTTCTTCGTGACCGAACGTGTAAATTTTTTGGATGATTTTGTGGAAATTGAGCTTTCGGAATCTGGTATTTGATTGTCAATTTGTAAATTTCTTGTCCTTGACTTCAATTTTGACACATTTCGTGAGGGATATTAGAGATCCGGAGCATCCGTATTCGTTAGAGCAGCTTAGTGTTCTCTCGGAGGAATCGATCACTGTCGATGAGAAGTTAGGGCGTATCCTGTAAGCTAAACTGCAACTCCTTTATTTTCTCTACCTCTTTGATTTTGAAAGACAATCTGAGATTATTGATTAATAATCGTATGGCTTGCACAAACACGTTCCTCGAGGATTTCTAGTGTAGCTTAAGTTTGATCGAAATGCCGTCAGCTTTGGATTCCTAAATTGTTTGCTGAATTAGTGTTTTTCTCACAGGATAACTTTTACTCCGACTATTCAGCATTGTAGTATGGCGACTGTGATTGGACTCTGTCTGAGAGTGAAACTTAAACATTTCTTTCCTCCACATTACAAGGTCAATTCACATTCCTTTCTTTAGCTACAAACTTGAAACATTGACGTACTGCTTCTTCAATTTCATTTCTCCACTCTACTTGATGCAATGCAATCACCAGATACTAATGGAATCGTGGCTTCTAAATTAATGTTCTCTGTATTCTCAATTTTAGGTGCCTGAACTTTGAAATTAATGTCTGATTCTCCGAATTTTCGGCCTTAATCTTTTAAGAACTCCTTAAATTTAGGTCCTTAACCAACCTGTTTTAAAATTTACTAACTAACTTATGAACATAAAATTGAAAGTTTAATAACTTAAGAGATAATAAATTGTACATCTTATGTACTTGTACATTTTTTAAAAAATTATTGAATAGGCTAAAGATTTGTTAGACATATAATTGAACGTTGAGTGACTTTTGGAGACCTATTAAACAAAACCTTACATTTAATGCAAATTCAAAGTTTAGGGGTTAAATTGGATGTTCTTAGTTTTTGAGGGAAACGATCATGAGAATAGTGCCTTTGGGGTCCATTTCACTTGCAAGGACTGCATGGGGGTAAATTTTAGGTGATTTCTTTTGGGGCTTAGTTTATAAACTTGGATTATATCAGGTTACCATTTAACAAAACTAAGGAGAAGCTTATTGTTCAAGAAAATTAGTTGTTAATGAAAATTGAAAACTAAAAACTTGTTTTCTGTTTTTGAAAATTGGCCAATAATGCAATTTTTTTACTTAAGATTGATAAACCATGGTAAGAAAATGAAAGAAAATAGACATCTATTTCAAAAATCAAGAATGGTTACCAATTGAGACTTTATATTATATATAAAGACAGATACATTCCCCATCTCAATCTTCCCGTCTTCAATTTGCCTTTTAGGTGATTTGATGATATTCGTACAACCATGAAGTCAATTTGAATGTTTACCTTACTTGTACAGGATCTACTCTATAGGTTGTATAATTGTATCATTGAGTTTTCTGACATATTTATTTATATATATAACTCAAGTTTCGATTGTCCACTACAATTTTTTTATCATGGATTTTGCAGGTTGACATCAAAGTGTCTCCAGGATCACACGCAAATGAAGACTCAGGTGAGATTAAAACATTCTAAAGTGTTATTGGATCAAAATACCCCTTGGAGAAAAGATCATGATATCGGCGATTGAGTTTTTTAGAAGCCTTAAATATTTGATAACTATTACTTCTTACTAACAGCATTAATCTGTCGTTTTTTACCCAACTGCTTGGATTGAACCAATAGTTAAATAGAGTTACATTTTAGTTATTATTTTCCTTTTTTGGAAGGAAATAGCTATTTGATTTAAATTATTATTGTCGGGTAAGTCTGTTAACCATTTGGTGTTTTTAGAAATTAAGCTTATAAACACATCGTTCCTAGAAAAAAACTAAGCATAAACTTAAAGAAGATTAAACTGAAAATGAAAGTGTCACCTAATGTAGCTTTAATAAACTTATATGAATAATATAATTAGAACGAACATGAATAACTGTTTGTTCTTTTTTTACGATGAAAGAACAGTTAACAAGCAGCTAAATGACAAAGAAAGAGTTGCAGCTGCAATGGAAAATCCAAATCTGCGTCAGCTTGTAGATGAATGCCTTTATTCCAGTGAGCTCTGAATCAAATCAATTATCAACGTCCAACGGGCCAAAAATGAAGGTAGTCTTCCAATTTAATGAAATATAAATTACATTTCAACTTTGTCTTCTGGCTTATAATTTTTTCAAATAGAATACACATTAGTCCTGAAAAAGTTCTCAAAGTGCATCTCTTTAACAATAATTTTATTCTTAAAGTTCAAGAACATAATTAAAATCTATTCCAAAGTAGAGATATTCATATAAATTAACATACAATACATATGATTATTTACTTTTTTTTTTTTGTTCTTTTGTTCTTTTGTTTTTGATTCTCACTCTCTGGAAATGAAACTCTTCGTTGTGCAAAAAATCAAGTTCAACGCACTATTGGGTACTTCAATTTTGATCCAAACAAATCATCTTTTCATCAAATGGGGAGGTACATGAGTTTATTATATAATACCTGTTCATTTATTTGCTATTTCTGCCTGTATATGTGAATGAACTTTTATCTGAGTTACTAATATATTATAGTTTAACGTTTCTTCAAATTTCTGTGTTTGTATTAAAAAAAAGTTTTTAATTTAAAAAAATACGGGGAAGATTCTTTTACTAATTCATCTCTCCGTAAGAATTTTAGATTTATTGGTTGAGCCTTTACAACTTGAAGTGGGTTTGTGTTTATCAACAAATCCATTTGCCATGGGTTTGTTATTTGTTTTTAGCTGTGTGTTTTTTGGGTTGAAGAAATTTGAATGATGAGTTGAGAATTTTATTAAACTAAGGAGATGGTATTTTCAAATTGAAATAGAAAAATAAAGTGTTGTTTCTTTCATATGTAGGAAAAACTGAGCATTAGCAAATGAGTGTTTTGCTTTTCTTGCATTTAGTATTTAAGTCGTATTCCAAAAAAACAAGAACAAGTTTTTTTTTTTTTTTTTAAAAAAAGATATGTATTCTAATTTTCAAGATTTGAATGTAAAGCATTCATTCATTAGTACAAGAAGAATTGTAAAATAAATGGGATAAATTGATCGGACCTAGTCTAAAATCGATTCTTGTTAAATTTAAAATAAAGGTAGCAGTATAATTGATCATAAATTCACACATAAATTTAAGTTAGTCTAGTATAGATTAGTTTTTTTTTTCCACTTCAAAAAGATTAAAAAAGACAATCAAATAGTTGTCAAAACTTTAATCTATAAAATTCACTCAATTTCTTCTTTCCTTTTCTATCTTTATTATTATCATTATTATTATTTAAATATCTCAAAAACTCATATATAGGCCGTTTTGGTTGGCCTATAGACATGGTTTCAAGAGCTATATTGGTCTATGGCATCACAGTTAGTTACATCCTGTAGCTGCCGACGTTTGACATATGAGCTTTTGATGCATGGAGTTGAGTTGAGTAATAATTATGATAATAACATACTCTGACATTTTAATAGATCAAGTTAGGCCAGAAAAGGAATATATGATATATGAAATTGGCTGTAATTCGAAGCCTAAACTTAAAGGCATGTGTCTCTCTAAGTTTATTATAAATGATACATGGAAAAAAATATGCAACAAAGTAAAGAAATTATAGTAATTTTCTTCTATTCTAAAGAGAGACAGCTACAAGAGAGAAATCAACAAAAGGTATCCCTCCATGTATTTATTCTTTTTTTTTAGAAAACATTATTGGGCTCTCTTGATAATTCTACTGTGTAAGTTAACACAATATATATAACAATAGTCTCTCATGGTTGGTTGGCAGAGACTAATTACTTATGGATATGAGCAAACATCATCTCCTTTGCATTAAAGAGAAGTTGATTATTATTTAACTTTCAAAATGGGCTGTAAAAAGGGGAGAGGCATGAGAGACATACCAGTTAGTTAGTTAGTCAGTTAGTTGGCGTAACGGGCTCAGGTCCAACTTCTGGGCCAGAGGAAGGCCCATTAACACTGTGTTGGGCCTTCTCATCAAAGCCCCAGAATTGGGCAGCTTTAAGCTCGTCTTGGCCCATCATCTGGGAGAACTGCTGATGATCATATTCGAGTGTGGCTTTACCTCCAAACACACTGGGAAGATTTTCCATGTCGAAGTGTGATTTCATCAGCTCCACACTTCCTTTGTCTTTCGGATTTACAAACTTTACCTTTTGAAATGTCTTTGGATCCAAGAAGTACTTTATAGCCTATAAAAATTGGAACAATCAATCTACTGGTTATTTCATTCGTGACAAAATAGGAAGGAAGTTTGGAGTTGATTGTTCGATCGTGTCCACATTTTCTATAAAGATATTAATATTGATGATACTTGAAAAGAAAATCATGGGAATTAACTTTTACTATTTGAATTAATAAAGGCACACTTAAACTTTATCCTTAGTGTACATACTGATTACTTTTTGTTGGTATTCAGATCCATAGATTGTCTTGAAATATTACGAACTATTGAGAGAAAGCTAAAAGCAGTAGGAATATTCAATTGTATGGATGCATGTATATGAATGATGGCATTAAAGAGCACATACCTTCCAGAAGGCTTGAAAAAACTTGGGGGGATTGTAGAGGAAAGCAAAGGCAAGTCTCTCGGGGTAATGGTTCTGAAGGACATTGATAATATCTGCGGCTATTTTGACAGAGACATTGGTTTTCATTGTAAATCCGGTGAAATCTATTAACCAACACATTTGTTCCTGACCCTCACCAAGGTTCATAATCGCATTCTCTAAAAGATAGACCAAATGCCGCACACTGGCTTCTGATGGAGTTGTGTTCTGAGAGATTTCAAAAATAGGAACTGTCACTAAAATCAGAAAGTGAATGCAAAACACAAAAAAGATCAAAACTTAAATTTGCAAATGAATGGACCACTTCTATACTGTGTACTTTTTATTTTTCTAAAAATTTGTCTACTTATTATTTCTTTCTGATGAGTGGTGGGAGGATTTGATATAATGCCTTAGTTTAGTCATGTAGAATTTGATAAATATCTTATCTAAACCTAGATGATCATTCAGATGCTTCTCTCT

Coding sequence (CDS)

ATGACGCTGGGGCTTATCAATGCGAATCCAGTGGTTCACGCCAAGAAGGAGCGGATCGCTCGCTCGGAAGACTTCCACGGTGACGACGCTGTTGATCCTCTTGAAATCTATGATTTCGTGAGGGATATTAGAGATCCGGAGCATCCGTATTCGTTAGAGCAGCTTAGTGTTCTCTCGGAGGAATCGATCACTGTCGATGAGAAGTTAGGGCGTATCCTGATAACTTTTACTCCGACTATTCAGCATTGTAGTATGGCGACTGTGATTGGACTCTGTCTGAGAGTGAAACTTAAACATTTCTTTCCTCCACATTACAAGGTTGACATCAAAGTGTCTCCAGGATCACACGCAAATGAAGACTCAGTTAACAAGCAGCTAAATGACAAAGAAAGAGTTGCAGCTGCAATGGAAAATCCAAATCTGCGTCAGCTTGTAGATGAATGCCTTTATTCCAGTGAGCTCTGA

Protein sequence

MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSEESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEDSVNKQLNDKERVAAAMENPNLRQLVDECLYSSEL

Homology

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BLAST of Cucsat.G12022.T4 vs. ExPASy Swiss-Prot
Match: A8MR89 (Protein AE7-like 1 OS=Arabidopsis thaliana OX=3702 GN=AEL1 PE=3 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 2.3e-65
Identity = 122/154 (79.22%), Postives = 135/154 (87.66%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60
           MTLGLINANPVV AKKE + R ED + DD VDPLEIYD+VRDIRDPEHPY+LEQL V+SE
Sbjct: 1   MTLGLINANPVVQAKKEGLVRREDQYRDDGVDPLEIYDYVRDIRDPEHPYTLEQLRVVSE 60

Query: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED 120
           ES+TVD+KL RILITFTPTIQHCSMA +IGLCLR KLK     HYKVDI+VSPGSHA+E 
Sbjct: 61  ESVTVDDKLDRILITFTPTIQHCSMANIIGLCLRAKLKECLQLHYKVDIRVSPGSHADEV 120

Query: 121 SVNKQLNDKERVAAAMENPNLRQLVDECLYSSEL 155
           SVNKQLNDKERV AA+ENPNLRQLVDEC+YS E+
Sbjct: 121 SVNKQLNDKERVVAALENPNLRQLVDECIYSDEI 154

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BLAST of Cucsat.G12022.T4 vs. ExPASy Swiss-Prot
Match: Q9C9G6 (Protein AE7 OS=Arabidopsis thaliana OX=3702 GN=AE7 PE=1 SV=2)

HSP 1 Score: 193.0 bits (489), Expect = 2.6e-48
Identity = 92/157 (58.60%), Postives = 122/157 (77.71%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGD----DAVDPLEIYDFVRDIRDPEHPYSLEQLS 60
           M  GLIN NP+++ KKER  R++    D    + +D LEI+D +RDI+DPEHP +LE L 
Sbjct: 1   MVSGLINENPIIYPKKERRLRTDTSITDELTPEPIDQLEIFDHIRDIKDPEHPNTLEDLR 60

Query: 61  VLSEESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSH 120
           V++E+S+ VD++   + +TFTPT++HCSMATVIGLC+RVKL    P  YK+DI+V+PGSH
Sbjct: 61  VVTEDSVEVDDENSYVRVTFTPTVEHCSMATVIGLCVRVKLLRSLPSRYKIDIRVAPGSH 120

Query: 121 ANEDSVNKQLNDKERVAAAMENPNLRQLVDECLYSSE 154
           A ED++NKQLNDKERVAAA+ENPNL ++VDECL S E
Sbjct: 121 ATEDALNKQLNDKERVAAALENPNLVEMVDECLPSEE 157

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BLAST of Cucsat.G12022.T4 vs. ExPASy Swiss-Prot
Match: O62252 (MIP18 family protein F45G2.10 OS=Caenorhabditis elegans OX=6239 GN=F45G2.10 PE=1 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 3.2e-35
Identity = 79/151 (52.32%), Postives = 100/151 (66.23%), Query Frame = 0


Query: 5   LINANPVVHAKKER----IARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 64
           L NANP +   K R         D   +D +D  EI+D +RDI DPEHPY+LEQL+V+ E
Sbjct: 6   LDNANPTLFDSKPRHRPVTGTERDESVEDPIDSWEIFDLIRDINDPEHPYTLEQLNVVQE 65

Query: 65  ESITV--DEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHAN 124
           E I V  DE+   + + FTPTI HCSMAT+IGL +RVKL     P  KV + ++PGSH+ 
Sbjct: 66  ELIKVFIDEEETFVKVNFTPTIPHCSMATLIGLAIRVKLLRSLHPKVKVSVSITPGSHST 125

Query: 125 EDSVNKQLNDKERVAAAMENPNLRQLVDECL 150
           E+S+N+QL DKERVAAAMEN  L   V+ECL
Sbjct: 126 EESINRQLADKERVAAAMENQGLMHAVNECL 156

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BLAST of Cucsat.G12022.T4 vs. ExPASy Swiss-Prot
Match: Q9VTC4 (MIP18 family protein galla-2 OS=Drosophila melanogaster OX=7227 GN=galla-2 PE=1 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 9.4e-35
Identity = 73/146 (50.00%), Postives = 107/146 (73.29%), Query Frame = 0


Query: 7   NANPVVHAK-KERI--ARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSEESI 66
           N NP V+ + KER+  A  ED +  D  D  EI+D +R+I DPEHP +LE+L V+ E+ I
Sbjct: 7   NINPNVYDRIKERVLTANEEDENVPDPFDKREIFDLIRNINDPEHPLTLEELHVVQEDLI 66

Query: 67  TVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEDSVN 126
            +++    + I+FTPTI HCSMAT+IGL +RVKL    PP +KV ++++PG+HA+E +VN
Sbjct: 67  RINDSQNSVHISFTPTIPHCSMATLIGLSIRVKLLRSLPPRFKVTVEITPGTHASELAVN 126

Query: 127 KQLNDKERVAAAMENPNLRQLVDECL 150
           KQL DKERVAAA+EN +L +++++C+
Sbjct: 127 KQLADKERVAAALENNHLAEVINQCI 152

[Close]

BLAST of Cucsat.G12022.T4 vs. ExPASy Swiss-Prot
Match: Q9D187 (Cytosolic iron-sulfur assembly component 2B OS=Mus musculus OX=10090 GN=Ciao2b PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 4.7e-34
Identity = 72/148 (48.65%), Postives = 105/148 (70.95%), Query Frame = 0


Query: 5   LINANPVVHAKK-ER--IARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSEE 64
           L NANP+++ +  ER   A  ED    D++D  EI+D +R I DPEHP +LE+L+V+ + 
Sbjct: 12  LENANPLIYERSGERPVTAGEEDEEVPDSIDAREIFDLIRSINDPEHPLTLEELNVVEQV 71

Query: 65  SITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEDS 124
            I V +    + + FTPTI HCSMAT+IGL ++VKL    P  +K+D+ ++PG+HA+E +
Sbjct: 72  RIQVSDPESTVAVAFTPTIPHCSMATLIGLSIKVKLLRSLPQRFKMDVHITPGTHASEHA 131

Query: 125 VNKQLNDKERVAAAMENPNLRQLVDECL 150
           VNKQL DKERVAAA+EN +L ++V++CL
Sbjct: 132 VNKQLADKERVAAALENTHLLEVVNQCL 159

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BLAST of Cucsat.G12022.T4 vs. NCBI nr
Match: XP_004149152.1 (protein AE7-like 1 [Cucumis sativus] >XP_008453741.1 PREDICTED: protein AE7-like 1 [Cucumis melo] >XP_031740257.1 protein AE7-like 1 [Cucumis sativus] >XP_038893023.1 protein AE7-like 1 [Benincasa hispida] >XP_038893030.1 protein AE7-like 1 [Benincasa hispida])

HSP 1 Score: 307 bits (787), Expect = 2.21e-105
Identity = 154/154 (100.00%), Postives = 154/154 (100.00%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60
           MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE
Sbjct: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60

Query: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED 120
           ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED
Sbjct: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED 120

Query: 121 SVNKQLNDKERVAAAMENPNLRQLVDECLYSSEL 154
           SVNKQLNDKERVAAAMENPNLRQLVDECLYSSEL
Sbjct: 121 SVNKQLNDKERVAAAMENPNLRQLVDECLYSSEL 154

[Close]

BLAST of Cucsat.G12022.T4 vs. NCBI nr
Match: XP_022136890.1 (protein AE7-like 1 [Momordica charantia])

HSP 1 Score: 300 bits (768), Expect = 1.75e-102
Identity = 150/154 (97.40%), Postives = 152/154 (98.70%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60
           MTLGLINANPVVHAKKERIARSED H DDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE
Sbjct: 1   MTLGLINANPVVHAKKERIARSEDLHADDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60

Query: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED 120
           ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANE+
Sbjct: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEE 120

Query: 121 SVNKQLNDKERVAAAMENPNLRQLVDECLYSSEL 154
           SVNKQLNDKERVAAA+ENPNLRQLVDECLYSSEL
Sbjct: 121 SVNKQLNDKERVAAALENPNLRQLVDECLYSSEL 154

[Close]

BLAST of Cucsat.G12022.T4 vs. NCBI nr
Match: XP_022984190.1 (protein AE7-like 1 isoform X2 [Cucurbita maxima])

HSP 1 Score: 298 bits (762), Expect = 1.44e-101
Identity = 149/153 (97.39%), Postives = 152/153 (99.35%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60
           MTLGLINANPVVHAKKERIARSE+FHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE
Sbjct: 1   MTLGLINANPVVHAKKERIARSEEFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60

Query: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED 120
           ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANE+
Sbjct: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEE 120

Query: 121 SVNKQLNDKERVAAAMENPNLRQLVDECLYSSE 153
           SVNKQLNDKERVAAA+ENPNLRQLVDECL SSE
Sbjct: 121 SVNKQLNDKERVAAALENPNLRQLVDECLDSSE 153

[Close]

BLAST of Cucsat.G12022.T4 vs. NCBI nr
Match: XP_015889928.1 (protein AE7-like 1 [Ziziphus jujuba])

HSP 1 Score: 297 bits (761), Expect = 2.04e-101
Identity = 146/154 (94.81%), Postives = 153/154 (99.35%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60
           MTLGLINANPVVHAKKER+AR+ED HGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE
Sbjct: 1   MTLGLINANPVVHAKKERVARTEDLHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60

Query: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED 120
           ESITVD+KLGRILITFTPTIQHCSMATVIGLCLRVKLKH+FPPHYKVDIKVSPGSHANE+
Sbjct: 61  ESITVDDKLGRILITFTPTIQHCSMATVIGLCLRVKLKHYFPPHYKVDIKVSPGSHANEE 120

Query: 121 SVNKQLNDKERVAAAMENPNLRQLVDECLYSSEL 154
           SVNKQLNDKERVAAA+ENPNLRQLVDECLYS+EL
Sbjct: 121 SVNKQLNDKERVAAALENPNLRQLVDECLYSNEL 154

[Close]

BLAST of Cucsat.G12022.T4 vs. NCBI nr
Match: XP_023551936.1 (protein AE7-like 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 297 bits (761), Expect = 2.04e-101
Identity = 149/153 (97.39%), Postives = 152/153 (99.35%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60
           MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE
Sbjct: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60

Query: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED 120
           ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANE+
Sbjct: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEE 120

Query: 121 SVNKQLNDKERVAAAMENPNLRQLVDECLYSSE 153
           SVNKQLNDKERVAAA+E+PNLRQLVDECL SSE
Sbjct: 121 SVNKQLNDKERVAAALEDPNLRQLVDECLDSSE 153

[Close]

BLAST of Cucsat.G12022.T4 vs. ExPASy TrEMBL
Match: A0A0A0KUM2 (FeS_assembly_P domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G038660 PE=3 SV=1)

HSP 1 Score: 307 bits (787), Expect = 1.07e-105
Identity = 154/154 (100.00%), Postives = 154/154 (100.00%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60
           MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE
Sbjct: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60

Query: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED 120
           ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED
Sbjct: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED 120

Query: 121 SVNKQLNDKERVAAAMENPNLRQLVDECLYSSEL 154
           SVNKQLNDKERVAAAMENPNLRQLVDECLYSSEL
Sbjct: 121 SVNKQLNDKERVAAAMENPNLRQLVDECLYSSEL 154

[Close]

BLAST of Cucsat.G12022.T4 vs. ExPASy TrEMBL
Match: A0A1S3BWZ8 (protein AE7-like 1 OS=Cucumis melo OX=3656 GN=LOC103494387 PE=3 SV=1)

HSP 1 Score: 307 bits (787), Expect = 1.07e-105
Identity = 154/154 (100.00%), Postives = 154/154 (100.00%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60
           MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE
Sbjct: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60

Query: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED 120
           ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED
Sbjct: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED 120

Query: 121 SVNKQLNDKERVAAAMENPNLRQLVDECLYSSEL 154
           SVNKQLNDKERVAAAMENPNLRQLVDECLYSSEL
Sbjct: 121 SVNKQLNDKERVAAAMENPNLRQLVDECLYSSEL 154

[Close]

BLAST of Cucsat.G12022.T4 vs. ExPASy TrEMBL
Match: A0A6J1C573 (protein AE7-like 1 OS=Momordica charantia OX=3673 GN=LOC111008470 PE=3 SV=1)

HSP 1 Score: 300 bits (768), Expect = 8.45e-103
Identity = 150/154 (97.40%), Postives = 152/154 (98.70%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60
           MTLGLINANPVVHAKKERIARSED H DDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE
Sbjct: 1   MTLGLINANPVVHAKKERIARSEDLHADDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60

Query: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED 120
           ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANE+
Sbjct: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEE 120

Query: 121 SVNKQLNDKERVAAAMENPNLRQLVDECLYSSEL 154
           SVNKQLNDKERVAAA+ENPNLRQLVDECLYSSEL
Sbjct: 121 SVNKQLNDKERVAAALENPNLRQLVDECLYSSEL 154

[Close]

BLAST of Cucsat.G12022.T4 vs. ExPASy TrEMBL
Match: A0A6J1J802 (protein AE7-like 1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111482581 PE=3 SV=1)

HSP 1 Score: 298 bits (762), Expect = 6.95e-102
Identity = 149/153 (97.39%), Postives = 152/153 (99.35%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60
           MTLGLINANPVVHAKKERIARSE+FHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE
Sbjct: 1   MTLGLINANPVVHAKKERIARSEEFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60

Query: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED 120
           ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANE+
Sbjct: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEE 120

Query: 121 SVNKQLNDKERVAAAMENPNLRQLVDECLYSSE 153
           SVNKQLNDKERVAAA+ENPNLRQLVDECL SSE
Sbjct: 121 SVNKQLNDKERVAAALENPNLRQLVDECLDSSE 153

[Close]

BLAST of Cucsat.G12022.T4 vs. ExPASy TrEMBL
Match: A0A6P4A8B7 (protein AE7-like 1 OS=Ziziphus jujuba OX=326968 GN=LOC107424594 PE=3 SV=1)

HSP 1 Score: 297 bits (761), Expect = 9.87e-102
Identity = 146/154 (94.81%), Postives = 153/154 (99.35%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60
           MTLGLINANPVVHAKKER+AR+ED HGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE
Sbjct: 1   MTLGLINANPVVHAKKERVARTEDLHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60

Query: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED 120
           ESITVD+KLGRILITFTPTIQHCSMATVIGLCLRVKLKH+FPPHYKVDIKVSPGSHANE+
Sbjct: 61  ESITVDDKLGRILITFTPTIQHCSMATVIGLCLRVKLKHYFPPHYKVDIKVSPGSHANEE 120

Query: 121 SVNKQLNDKERVAAAMENPNLRQLVDECLYSSEL 154
           SVNKQLNDKERVAAA+ENPNLRQLVDECLYS+EL
Sbjct: 121 SVNKQLNDKERVAAALENPNLRQLVDECLYSNEL 154

The following BLAST results are available for this feature:

BLAST of Cucsat.G12022.T4 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A8MR89	2.3e-65	79.22	Protein AE7-like 1 OS=Arabidopsis thaliana OX=3702 GN=AEL1 PE=3 SV=1	[more]
Q9C9G6	2.6e-48	58.60	Protein AE7 OS=Arabidopsis thaliana OX=3702 GN=AE7 PE=1 SV=2	[more]
O62252	3.2e-35	52.32	MIP18 family protein F45G2.10 OS=Caenorhabditis elegans OX=6239 GN=F45G2.10 PE=1...	[more]
Q9VTC4	9.4e-35	50.00	MIP18 family protein galla-2 OS=Drosophila melanogaster OX=7227 GN=galla-2 PE=1 ...	[more]
Q9D187	4.7e-34	48.65	Cytosolic iron-sulfur assembly component 2B OS=Mus musculus OX=10090 GN=Ciao2b P...	[more]

BLAST of Cucsat.G12022.T4 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004149152.1	2.21e-105	100.00	protein AE7-like 1 [Cucumis sativus] >XP_008453741.1 PREDICTED: protein AE7-like...	[more]
XP_022136890.1	1.75e-102	97.40	protein AE7-like 1 [Momordica charantia]	[more]
XP_022984190.1	1.44e-101	97.39	protein AE7-like 1 isoform X2 [Cucurbita maxima]	[more]
XP_015889928.1	2.04e-101	94.81	protein AE7-like 1 [Ziziphus jujuba]	[more]
XP_023551936.1	2.04e-101	97.39	protein AE7-like 1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cucsat.G12022.T4 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KUM2	1.07e-105	100.00	FeS_assembly_P domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G038...	[more]
A0A1S3BWZ8	1.07e-105	100.00	protein AE7-like 1 OS=Cucumis melo OX=3656 GN=LOC103494387 PE=3 SV=1	[more]
A0A6J1C573	8.45e-103	97.40	protein AE7-like 1 OS=Momordica charantia OX=3673 GN=LOC111008470 PE=3 SV=1	[more]
A0A6J1J802	6.95e-102	97.39	protein AE7-like 1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111482581 PE=3 S...	[more]
A0A6P4A8B7	9.87e-102	94.81	protein AE7-like 1 OS=Ziziphus jujuba OX=326968 GN=LOC107424594 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002744	MIP18 family-like	PFAM	PF01883	FeS_assembly_P	coord: 35..103 e-value: 1.0E-8 score: 35.3
None	No IPR available	GENE3D	6.10.250.1280		coord: 118..149 e-value: 1.6E-18 score: 68.1
None	No IPR available	PANTHER	PTHR12377:SF3	PROTEIN AE7-LIKE 1	coord: 1..153
IPR034904	Fe-S cluster assembly domain superfamily	GENE3D	3.30.300.130		coord: 28..117 e-value: 6.1E-34 score: 117.7
IPR034904	Fe-S cluster assembly domain superfamily	SUPERFAMILY	117916	Fe-S cluster assembly (FSCA) domain-like	coord: 35..125
IPR039796	MIP18 family	PANTHER	PTHR12377	UNCHARACTERIZED	coord: 1..153

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G12022	Cucsat.G12022	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G12022.T4.E1	Cucsat.G12022.T4.E1	exon
Cucsat.G12022.T4.E2	Cucsat.G12022.T4.E2	exon
Cucsat.G12022.T4.E3	Cucsat.G12022.T4.E3	exon
Cucsat.G12022.T4.E4	Cucsat.G12022.T4.E4	exon
Cucsat.G12022.T4.E5	Cucsat.G12022.T4.E5	exon
Cucsat.G12022.T4.E6	Cucsat.G12022.T4.E6	exon
Cucsat.G12022.T4.E7	Cucsat.G12022.T4.E7	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G12022.T4.C5	Cucsat.G12022.T4.C5	CDS
Cucsat.G12022.T4.C4	Cucsat.G12022.T4.C4	CDS
Cucsat.G12022.T4.C3	Cucsat.G12022.T4.C3	CDS
Cucsat.G12022.T4.C2	Cucsat.G12022.T4.C2	CDS
Cucsat.G12022.T4.C1	Cucsat.G12022.T4.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G12022.T4	Cucsat.G12022.T4-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0106035	protein maturation by [4Fe-4S] cluster transfer
biological_process	GO:0016226	iron-sulfur cluster assembly
cellular_component	GO:0016021	integral component of membrane