Cucsat.G12022.T2 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G12022.T2
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	protein AE7-like 1
Location	ctg1820: 5276053 .. 5281658 (-)
RNA-Seq Expression	Cucsat.G12022.T2
Synteny	Cucsat.G12022.T2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TATACCGTTACGTCACCCATTAATTTGACCAAGATTCCAACAAATCACCATTACGTGCTTCTCTCTTACTGGACTGTCACGCGCCTTTTCCAGCGGTGACGGATATGTTTAATGTTTAAACTAGTTTAGTATAGTTTATAGTTTATACCCACTAGTCTGTGATTAAAACTTCACCGAAACAACAAGAAGGAAGAAGAAGAAGAAGAAGGGATGACGCTGGGGCTTATCAATGCGAATCCAGTGGTTCACGCCAAGAAGGAGCGGATCGCTCGCTCGGAAGACTTCCACGGTGACGACGCTGTTGATCCTCTTGAAATCTATGATATCCTTTTCTTAACTGTTTGTGTTTTTACTAAATTTTGAGTTCTCCTTTCATTTTATTTGAACTTCAATTTTCGCTGTGGTTTCTTGCTTAATTCTGATTATCAAGTTAATTAGATTCCGTTTTGTCTGTATGCTTTTGGAGGATTCTCTTGTTTTCTTTTAGAATTTTCTAGCGCTTTCTTCGTGACCGAACGTGTAAATTTTTTGGATGATTTTGTGGAAATTGAGCTTTCGGAATCTGGTATTTGATTGTCAATTTGTAAATTTCTTGTCCTTGACTTCAATTTTGACACATTTCGTGAGGGATATTAGAGATCCGGAGCATCCGTATTCGTTAGAGCAGCTTAGTGTTCTCTCGGAGGAATCGATCACTGTCGATGAGAAGTTAGGGCGTATCCTGTAAGCTAAACTGCAACTCCTTTATTTTCTCTACCTCTTTGATTTTGAAAGACAATCTGAGATTATTGATTAATAATCGTATGGCTTGCACAAACACGTTCCTCGAGGATTTCTAGTGTAGCTTAAGTTTGATCGAAATGCCGTCAGCTTTGGATTCCTAAATTGTTTGCTGAATTAGTGTTTTTCTCACAGGATAACTTTTACTCCGACTATTCAGCATTGTAGTATGGCGACTGTGATTGGACTCTGTCTGAGAGTGAAACTTAAACATTTCTTTCCTCCACATTACAAGGTCAATTCACATTCCTTTCTTTAGCTACAAACTTGAAACATTGACGTACTGCTTCTTCAATTTCATTTCTCCACTCTACTTGATGCAATGCAATCACCAGATACTAATGGAATCGTGGCTTCTAAATTAATGTTCTCTGTATTCTCAATTTTAGGTGCCTGAACTTTGAAATTAATGTCTGATTCTCCGAATTTTCGGCCTTAATCTTTTAAGAACTCCTTAAATTTAGGTCCTTAACCAACCTGTTTTAAAATTTACTAACTAACTTATGAACATAAAATTGAAAGTTTAATAACTTAAGAGATAATAAATTGTACATCTTATGTACTTGTACATTTTTTAAAAAATTATTGAATAGGCTAAAGATTTGTTAGACATATAATTGAACGTTGAGTGACTTTTGGAGACCTATTAAACAAAACCTTACATTTAATGCAAATTCAAAGTTTAGGGGTTAAATTGGATGTTCTTAGTTTTTGAGGGAAACGATCATGAGAATAGTGCCTTTGGGGTCCATTTCACTTGCAAGGACTGCATGGGGGTAAATTTTAGGTGATTTCTTTTGGGGCTTAGTTTATAAACTTGGATTATATCAGGTTACCATTTAACAAAACTAAGGAGAAGCTTATTGTTCAAGAAAATTAGTTGTTAATGAAAATTGAAAACTAAAAACTTGTTTTCTGTTTTTGAAAATTGGCCAATAATGCAATTTTTTTACTTAAGATTGATAAACCATGGTAAGAAAATGAAAGAAAATAGACATCTATTTCAAAAATCAAGAATGGTTACCAATTGAGACTTTATATTATATATAAAGACAGATACATTCCCCATCTCAATCTTCCCGTCTTCAATTTGCCTTTTAGGTGATTTGATGATATTCGTACAACCATGAAGTCAATTTGAATGTTTACCTTACTTGTACAGGATCTACTCTATAGGTTGTATAATTGTATCATTGAGTTTTCTGACATATTTATTTATATATATAACTCAAGTTTCGATTGTCCACTACAATTTTTTTATCATGGATTTTGCAGGTTGACATCAAAGTGTCTCCAGGATCACACGCAAATGAAGACTCAGGTGAGATTAAAACATTCTAAAGTGTTATTGGATCAAAATACCCCTTGGAGAAAAGATCATGATATCGGCGATTGAGTTTTTTAGAAGCCTTAAATATTTGATAACTATTACTTCTTACTAACAGCATTAATCTGTCGTTTTTTACCCAACTGCTTGGATTGAACCAATAGTTAAATAGAGTTACATTTTAGTTATTATTTTCCTTTTTTGGAAGGAAATAGCTATTTGATTTAAATTATTATTGTCGGGTAAGTCTGTTAACCATTTGGTGTTTTTAGAAATTAAGCTTATAAACACATCGTTCCTAGAAAAAAACTAAGCATAAACTTAAAGAAGATTAAACTGAAAATGAAAGTGTCACCTAATGTAGCTTTAATAAACTTATATGAATAATATAATTAGAACGAACATGAATAACTGTTTGTTCTTTTTTTACGATGAAAGAACAGTTAACAAGCAGCTAAATGACAAAGAAAGAGTTGCAGCTGCAATGGAAAATCCAAATCTGCGTCAGCTTGTAGATGAATGCCTTTATTCCAGTGAGCTCTGAATCAAATCAATTATCAACGTCCAACGGGCCAAAAATGAAGGTAGTCTTCCAATTTAATGAAATATAAATTACATTTCAACTTTGTCTTCTGGCTTATAATTTTTTCAAATAGAATACACATTAGTCCTGAAAAAGTTCTCAAAGTGCATCTCTTTAACAATAATTTTATTCTTAAAGTTCAAGAACATAATTAAAATCTATTCCAAAGTAGAGATATTCATATAAATTAACATACAATACATATGATTATTTACTTTTTTTTTTTTGTTCTTTTGTTCTTTTGTTTTTGATTCTCACTCTCTGGAAATGAAACTCTTCGTTGTGCAAAAAATCAAGTTCAACGCACTATTGGGTACTTCAATTTTGATCCAAACAAATCATCTTTTCATCAAATGGGGAGGTACATGAGTTTATTATATAATACCTGTTCATTTATTTGCTATTTCTGCCTGTATATGTGAATGAACTTTTATCTGAGTTACTAATATATTATAGTTTAACGTTTCTTCAAATTTCTGTGTTTGTATTAAAAAAAAGTTTTTAATTTAAAAAAATACGGGGAAGATTCTTTTACTAATTCATCTCTCCGTAAGAATTTTAGATTTATTGGTTGAGCCTTTACAACTTGAAGTGGGTTTGTGTTTATCAACAAATCCATTTGCCATGGGTTTGTTATTTGTTTTTAGCTGTGTGTTTTTTGGGTTGAAGAAATTTGAATGATGAGTTGAGAATTTTATTAAACTAAGGAGATGGTATTTTCAAATTGAAATAGAAAAATAAAGTGTTGTTTCTTTCATATGTAGGAAAAACTGAGCATTAGCAAATGAGTGTTTTGCTTTTCTTGCATTTAGTATTTAAGTCGTATTCCAAAAAAACAAGAACAAGTTTTTTTTTTTTTTTTTAAAAAAAGATATGTATTCTAATTTTCAAGATTTGAATGTAAAGCATTCATTCATTAGTACAAGAAGAATTGTAAAATAAATGGGATAAATTGATCGGACCTAGTCTAAAATCGATTCTTGTTAAATTTAAAATAAAGGTAGCAGTATAATTGATCATAAATTCACACATAAATTTAAGTTAGTCTAGTATAGATTAGTTTTTTTTTTCCACTTCAAAAAGATTAAAAAAGACAATCAAATAGTTGTCAAAACTTTAATCTATAAAATTCACTCAATTTCTTCTTTCCTTTTCTATCTTTATTATTATCATTATTATTATTTAAATATCTCAAAAACTCATATATAGGCCGTTTTGGTTGGCCTATAGACATGGTTTCAAGAGCTATATTGGTCTATGGCATCACAGTTAGTTACATCCTGTAGCTGCCGACGTTTGACATATGAGCTTTTGATGCATGGAGTTGAGTTGAGTAATAATTATGATAATAACATACTCTGACATTTTAATAGATCAAGTTAGGCCAGAAAAGGAATATATGATATATGAAATTGGCTGTAATTCGAAGCCTAAACTTAAAGGCATGTGTCTCTCTAAGTTTATTATAAATGATACATGGAAAAAAATATGCAACAAAGTAAAGAAATTATAGTAATTTTCTTCTATTCTAAAGAGAGACAGCTACAAGAGAGAAATCAACAAAAGGTATCCCTCCATGTATTTATTCTTTTTTTTTAGAAAACATTATTGGGCTCTCTTGATAATTCTACTGTGTAAGTTAACACAATATATATAACAATAGTCTCTCATGGTTGGTTGGCAGAGACTAATTACTTATGGATATGAGCAAACATCATCTCCTTTGCATTAAAGAGAAGTTGATTATTATTTAACTTTCAAAATGGGCTGTAAAAAGGGGAGAGGCATGAGAGACATACCAGTTAGTTAGTTAGTCAGTTAGTTGGCGTAACGGGCTCAGGTCCAACTTCTGGGCCAGAGGAAGGCCCATTAACACTGTGTTGGGCCTTCTCATCAAAGCCCCAGAATTGGGCAGCTTTAAGCTCGTCTTGGCCCATCATCTGGGAGAACTGCTGATGATCATATTCGAGTGTGGCTTTACCTCCAAACACACTGGGAAGATTTTCCATGTCGAAGTGTGATTTCATCAGCTCCACACTTCCTTTGTCTTTCGGATTTACAAACTTTACCTTTTGAAATGTCTTTGGATCCAAGAAGTACTTTATAGCCTATAAAAATTGGAACAATCAATCTACTGGTTATTTCATTCGTGACAAAATAGGAAGGAAGTTTGGAGTTGATTGTTCGATCGTGTCCACATTTTCTATAAAGATATTAATATTGATGATACTTGAAAAGAAAATCATGGGAATTAACTTTTACTATTTGAATTAATAAAGGCACACTTAAACTTTATCCTTAGTGTACATACTGATTACTTTTTGTTGGTATTCAGATCCATAGATTGTCTTGAAATATTACGAACTATTGAGAGAAAGCTAAAAGCAGTAGGAATATTCAATTGTATGGATGCATGTATATGAATGATGGCATTAAAGAGCACATACCTTCCAGAAGGCTTGAAAAAACTTGGGGGGATTGTAGAGGAAAGCAAAGGCAAGTCTCTCGGGGTAATGGTTCTGAAGGACATTGATAATATCTGCGGCTATTTTGACAGAGACATTGGTTTTCATTGTAAATCCGGTGAAATCTATTAACCAACACATTTGTTCCTGACCCTCACCAAGGTTCATAATCGCATTCTCTAAAAGATAGACCAAATGCCGCACACTGGCTTCTGATGGAGTTGTGTTCTGAGAGATTTCAAAAATAGGAACTGTCACTAAAATCAGAAAGTGAATGCAAAACACAAAAAAGATCAAAACTTAAATTTGCAAATGAATGGACCACTTCTATACTGTGTACTTTTTATTTTTCTAAAAATTTGTCTACTTATTATTTCTTTCTGATGAGTGGTGGGAGGATTTGATATAATGCCTTAGTTTAGTCATGTAGAATTTGATAAATATCTTATCT

Coding sequence (CDS)

ATGACGCTGGGGCTTATCAATGCGAATCCAGTGGTTCACGCCAAGAAGGAGCGGATCGCTCGCTCGGAAGACTTCCACGGTGACGACGCTGTTGATCCTCTTGAAATCTATGATTTCGTGAGGGATATTAGAGATCCGGAGCATCCGTATTCGTTAGAGCAGCTTAGTGTTCTCTCGGAGGAATCGATCACTGTCGATGAGAAGTTAGGGCGTATCCTGATAACTTTTACTCCGACTATTCAGCATTGTAGTATGGCGACTGTGATTGGACTCTGTCTGAGAGTGAAACTTAAACATTTCTTTCCTCCACATTACAAGGTTGACATCAAAGTGTCTCCAGGATCACACGCAAATGAAGACTCAGTTAACAAGCAGCTAAATGACAAAGAAAGAGTTGCAGCTGCAATGGAAAATCCAAATCTGCGTCAGCTTGTAGATGAATGCCTTTATTCCAGTGAGCTCTGA

Protein sequence

MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSEESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEDSVNKQLNDKERVAAAMENPNLRQLVDECLYSSEL

Homology

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BLAST of Cucsat.G12022.T2 vs. ExPASy Swiss-Prot
Match: A8MR89 (Protein AE7-like 1 OS=Arabidopsis thaliana OX=3702 GN=AEL1 PE=3 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 2.3e-65
Identity = 122/154 (79.22%), Postives = 135/154 (87.66%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60
           MTLGLINANPVV AKKE + R ED + DD VDPLEIYD+VRDIRDPEHPY+LEQL V+SE
Sbjct: 1   MTLGLINANPVVQAKKEGLVRREDQYRDDGVDPLEIYDYVRDIRDPEHPYTLEQLRVVSE 60

Query: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED 120
           ES+TVD+KL RILITFTPTIQHCSMA +IGLCLR KLK     HYKVDI+VSPGSHA+E 
Sbjct: 61  ESVTVDDKLDRILITFTPTIQHCSMANIIGLCLRAKLKECLQLHYKVDIRVSPGSHADEV 120

Query: 121 SVNKQLNDKERVAAAMENPNLRQLVDECLYSSEL 155
           SVNKQLNDKERV AA+ENPNLRQLVDEC+YS E+
Sbjct: 121 SVNKQLNDKERVVAALENPNLRQLVDECIYSDEI 154

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BLAST of Cucsat.G12022.T2 vs. ExPASy Swiss-Prot
Match: Q9C9G6 (Protein AE7 OS=Arabidopsis thaliana OX=3702 GN=AE7 PE=1 SV=2)

HSP 1 Score: 193.0 bits (489), Expect = 2.6e-48
Identity = 92/157 (58.60%), Postives = 122/157 (77.71%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGD----DAVDPLEIYDFVRDIRDPEHPYSLEQLS 60
           M  GLIN NP+++ KKER  R++    D    + +D LEI+D +RDI+DPEHP +LE L 
Sbjct: 1   MVSGLINENPIIYPKKERRLRTDTSITDELTPEPIDQLEIFDHIRDIKDPEHPNTLEDLR 60

Query: 61  VLSEESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSH 120
           V++E+S+ VD++   + +TFTPT++HCSMATVIGLC+RVKL    P  YK+DI+V+PGSH
Sbjct: 61  VVTEDSVEVDDENSYVRVTFTPTVEHCSMATVIGLCVRVKLLRSLPSRYKIDIRVAPGSH 120

Query: 121 ANEDSVNKQLNDKERVAAAMENPNLRQLVDECLYSSE 154
           A ED++NKQLNDKERVAAA+ENPNL ++VDECL S E
Sbjct: 121 ATEDALNKQLNDKERVAAALENPNLVEMVDECLPSEE 157

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BLAST of Cucsat.G12022.T2 vs. ExPASy Swiss-Prot
Match: O62252 (MIP18 family protein F45G2.10 OS=Caenorhabditis elegans OX=6239 GN=F45G2.10 PE=1 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 3.2e-35
Identity = 79/151 (52.32%), Postives = 100/151 (66.23%), Query Frame = 0


Query: 5   LINANPVVHAKKER----IARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 64
           L NANP +   K R         D   +D +D  EI+D +RDI DPEHPY+LEQL+V+ E
Sbjct: 6   LDNANPTLFDSKPRHRPVTGTERDESVEDPIDSWEIFDLIRDINDPEHPYTLEQLNVVQE 65

Query: 65  ESITV--DEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHAN 124
           E I V  DE+   + + FTPTI HCSMAT+IGL +RVKL     P  KV + ++PGSH+ 
Sbjct: 66  ELIKVFIDEEETFVKVNFTPTIPHCSMATLIGLAIRVKLLRSLHPKVKVSVSITPGSHST 125

Query: 125 EDSVNKQLNDKERVAAAMENPNLRQLVDECL 150
           E+S+N+QL DKERVAAAMEN  L   V+ECL
Sbjct: 126 EESINRQLADKERVAAAMENQGLMHAVNECL 156

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BLAST of Cucsat.G12022.T2 vs. ExPASy Swiss-Prot
Match: Q9VTC4 (MIP18 family protein galla-2 OS=Drosophila melanogaster OX=7227 GN=galla-2 PE=1 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 9.4e-35
Identity = 73/146 (50.00%), Postives = 107/146 (73.29%), Query Frame = 0


Query: 7   NANPVVHAK-KERI--ARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSEESI 66
           N NP V+ + KER+  A  ED +  D  D  EI+D +R+I DPEHP +LE+L V+ E+ I
Sbjct: 7   NINPNVYDRIKERVLTANEEDENVPDPFDKREIFDLIRNINDPEHPLTLEELHVVQEDLI 66

Query: 67  TVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEDSVN 126
            +++    + I+FTPTI HCSMAT+IGL +RVKL    PP +KV ++++PG+HA+E +VN
Sbjct: 67  RINDSQNSVHISFTPTIPHCSMATLIGLSIRVKLLRSLPPRFKVTVEITPGTHASELAVN 126

Query: 127 KQLNDKERVAAAMENPNLRQLVDECL 150
           KQL DKERVAAA+EN +L +++++C+
Sbjct: 127 KQLADKERVAAALENNHLAEVINQCI 152

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BLAST of Cucsat.G12022.T2 vs. ExPASy Swiss-Prot
Match: Q9D187 (Cytosolic iron-sulfur assembly component 2B OS=Mus musculus OX=10090 GN=Ciao2b PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 4.7e-34
Identity = 72/148 (48.65%), Postives = 105/148 (70.95%), Query Frame = 0


Query: 5   LINANPVVHAKK-ER--IARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSEE 64
           L NANP+++ +  ER   A  ED    D++D  EI+D +R I DPEHP +LE+L+V+ + 
Sbjct: 12  LENANPLIYERSGERPVTAGEEDEEVPDSIDAREIFDLIRSINDPEHPLTLEELNVVEQV 71

Query: 65  SITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEDS 124
            I V +    + + FTPTI HCSMAT+IGL ++VKL    P  +K+D+ ++PG+HA+E +
Sbjct: 72  RIQVSDPESTVAVAFTPTIPHCSMATLIGLSIKVKLLRSLPQRFKMDVHITPGTHASEHA 131

Query: 125 VNKQLNDKERVAAAMENPNLRQLVDECL 150
           VNKQL DKERVAAA+EN +L ++V++CL
Sbjct: 132 VNKQLADKERVAAALENTHLLEVVNQCL 159

[Close]

BLAST of Cucsat.G12022.T2 vs. NCBI nr
Match: XP_004149152.1 (protein AE7-like 1 [Cucumis sativus] >XP_008453741.1 PREDICTED: protein AE7-like 1 [Cucumis melo] >XP_031740257.1 protein AE7-like 1 [Cucumis sativus] >XP_038893023.1 protein AE7-like 1 [Benincasa hispida] >XP_038893030.1 protein AE7-like 1 [Benincasa hispida])

HSP 1 Score: 307 bits (787), Expect = 2.21e-105
Identity = 154/154 (100.00%), Postives = 154/154 (100.00%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60
           MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE
Sbjct: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60

Query: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED 120
           ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED
Sbjct: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED 120

Query: 121 SVNKQLNDKERVAAAMENPNLRQLVDECLYSSEL 154
           SVNKQLNDKERVAAAMENPNLRQLVDECLYSSEL
Sbjct: 121 SVNKQLNDKERVAAAMENPNLRQLVDECLYSSEL 154

[Close]

BLAST of Cucsat.G12022.T2 vs. NCBI nr
Match: XP_022136890.1 (protein AE7-like 1 [Momordica charantia])

HSP 1 Score: 300 bits (768), Expect = 1.75e-102
Identity = 150/154 (97.40%), Postives = 152/154 (98.70%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60
           MTLGLINANPVVHAKKERIARSED H DDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE
Sbjct: 1   MTLGLINANPVVHAKKERIARSEDLHADDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60

Query: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED 120
           ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANE+
Sbjct: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEE 120

Query: 121 SVNKQLNDKERVAAAMENPNLRQLVDECLYSSEL 154
           SVNKQLNDKERVAAA+ENPNLRQLVDECLYSSEL
Sbjct: 121 SVNKQLNDKERVAAALENPNLRQLVDECLYSSEL 154

[Close]

BLAST of Cucsat.G12022.T2 vs. NCBI nr
Match: XP_022984190.1 (protein AE7-like 1 isoform X2 [Cucurbita maxima])

HSP 1 Score: 298 bits (762), Expect = 1.44e-101
Identity = 149/153 (97.39%), Postives = 152/153 (99.35%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60
           MTLGLINANPVVHAKKERIARSE+FHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE
Sbjct: 1   MTLGLINANPVVHAKKERIARSEEFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60

Query: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED 120
           ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANE+
Sbjct: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEE 120

Query: 121 SVNKQLNDKERVAAAMENPNLRQLVDECLYSSE 153
           SVNKQLNDKERVAAA+ENPNLRQLVDECL SSE
Sbjct: 121 SVNKQLNDKERVAAALENPNLRQLVDECLDSSE 153

[Close]

BLAST of Cucsat.G12022.T2 vs. NCBI nr
Match: XP_015889928.1 (protein AE7-like 1 [Ziziphus jujuba])

HSP 1 Score: 297 bits (761), Expect = 2.04e-101
Identity = 146/154 (94.81%), Postives = 153/154 (99.35%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60
           MTLGLINANPVVHAKKER+AR+ED HGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE
Sbjct: 1   MTLGLINANPVVHAKKERVARTEDLHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60

Query: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED 120
           ESITVD+KLGRILITFTPTIQHCSMATVIGLCLRVKLKH+FPPHYKVDIKVSPGSHANE+
Sbjct: 61  ESITVDDKLGRILITFTPTIQHCSMATVIGLCLRVKLKHYFPPHYKVDIKVSPGSHANEE 120

Query: 121 SVNKQLNDKERVAAAMENPNLRQLVDECLYSSEL 154
           SVNKQLNDKERVAAA+ENPNLRQLVDECLYS+EL
Sbjct: 121 SVNKQLNDKERVAAALENPNLRQLVDECLYSNEL 154

[Close]

BLAST of Cucsat.G12022.T2 vs. NCBI nr
Match: XP_023551936.1 (protein AE7-like 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 297 bits (761), Expect = 2.04e-101
Identity = 149/153 (97.39%), Postives = 152/153 (99.35%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60
           MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE
Sbjct: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60

Query: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED 120
           ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANE+
Sbjct: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEE 120

Query: 121 SVNKQLNDKERVAAAMENPNLRQLVDECLYSSE 153
           SVNKQLNDKERVAAA+E+PNLRQLVDECL SSE
Sbjct: 121 SVNKQLNDKERVAAALEDPNLRQLVDECLDSSE 153

[Close]

BLAST of Cucsat.G12022.T2 vs. ExPASy TrEMBL
Match: A0A0A0KUM2 (FeS_assembly_P domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G038660 PE=3 SV=1)

HSP 1 Score: 307 bits (787), Expect = 1.07e-105
Identity = 154/154 (100.00%), Postives = 154/154 (100.00%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60
           MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE
Sbjct: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60

Query: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED 120
           ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED
Sbjct: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED 120

Query: 121 SVNKQLNDKERVAAAMENPNLRQLVDECLYSSEL 154
           SVNKQLNDKERVAAAMENPNLRQLVDECLYSSEL
Sbjct: 121 SVNKQLNDKERVAAAMENPNLRQLVDECLYSSEL 154

[Close]

BLAST of Cucsat.G12022.T2 vs. ExPASy TrEMBL
Match: A0A1S3BWZ8 (protein AE7-like 1 OS=Cucumis melo OX=3656 GN=LOC103494387 PE=3 SV=1)

HSP 1 Score: 307 bits (787), Expect = 1.07e-105
Identity = 154/154 (100.00%), Postives = 154/154 (100.00%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60
           MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE
Sbjct: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60

Query: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED 120
           ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED
Sbjct: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED 120

Query: 121 SVNKQLNDKERVAAAMENPNLRQLVDECLYSSEL 154
           SVNKQLNDKERVAAAMENPNLRQLVDECLYSSEL
Sbjct: 121 SVNKQLNDKERVAAAMENPNLRQLVDECLYSSEL 154

[Close]

BLAST of Cucsat.G12022.T2 vs. ExPASy TrEMBL
Match: A0A6J1C573 (protein AE7-like 1 OS=Momordica charantia OX=3673 GN=LOC111008470 PE=3 SV=1)

HSP 1 Score: 300 bits (768), Expect = 8.45e-103
Identity = 150/154 (97.40%), Postives = 152/154 (98.70%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60
           MTLGLINANPVVHAKKERIARSED H DDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE
Sbjct: 1   MTLGLINANPVVHAKKERIARSEDLHADDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60

Query: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED 120
           ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANE+
Sbjct: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEE 120

Query: 121 SVNKQLNDKERVAAAMENPNLRQLVDECLYSSEL 154
           SVNKQLNDKERVAAA+ENPNLRQLVDECLYSSEL
Sbjct: 121 SVNKQLNDKERVAAALENPNLRQLVDECLYSSEL 154

[Close]

BLAST of Cucsat.G12022.T2 vs. ExPASy TrEMBL
Match: A0A6J1J802 (protein AE7-like 1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111482581 PE=3 SV=1)

HSP 1 Score: 298 bits (762), Expect = 6.95e-102
Identity = 149/153 (97.39%), Postives = 152/153 (99.35%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60
           MTLGLINANPVVHAKKERIARSE+FHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE
Sbjct: 1   MTLGLINANPVVHAKKERIARSEEFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60

Query: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED 120
           ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANE+
Sbjct: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEE 120

Query: 121 SVNKQLNDKERVAAAMENPNLRQLVDECLYSSE 153
           SVNKQLNDKERVAAA+ENPNLRQLVDECL SSE
Sbjct: 121 SVNKQLNDKERVAAALENPNLRQLVDECLDSSE 153

[Close]

BLAST of Cucsat.G12022.T2 vs. ExPASy TrEMBL
Match: A0A6P4A8B7 (protein AE7-like 1 OS=Ziziphus jujuba OX=326968 GN=LOC107424594 PE=3 SV=1)

HSP 1 Score: 297 bits (761), Expect = 9.87e-102
Identity = 146/154 (94.81%), Postives = 153/154 (99.35%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60
           MTLGLINANPVVHAKKER+AR+ED HGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE
Sbjct: 1   MTLGLINANPVVHAKKERVARTEDLHGDDAVDPLEIYDFVRDIRDPEHPYSLEQLSVLSE 60

Query: 61  ESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANED 120
           ESITVD+KLGRILITFTPTIQHCSMATVIGLCLRVKLKH+FPPHYKVDIKVSPGSHANE+
Sbjct: 61  ESITVDDKLGRILITFTPTIQHCSMATVIGLCLRVKLKHYFPPHYKVDIKVSPGSHANEE 120

Query: 121 SVNKQLNDKERVAAAMENPNLRQLVDECLYSSEL 154
           SVNKQLNDKERVAAA+ENPNLRQLVDECLYS+EL
Sbjct: 121 SVNKQLNDKERVAAALENPNLRQLVDECLYSNEL 154

The following BLAST results are available for this feature:

BLAST of Cucsat.G12022.T2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A8MR89	2.3e-65	79.22	Protein AE7-like 1 OS=Arabidopsis thaliana OX=3702 GN=AEL1 PE=3 SV=1	[more]
Q9C9G6	2.6e-48	58.60	Protein AE7 OS=Arabidopsis thaliana OX=3702 GN=AE7 PE=1 SV=2	[more]
O62252	3.2e-35	52.32	MIP18 family protein F45G2.10 OS=Caenorhabditis elegans OX=6239 GN=F45G2.10 PE=1...	[more]
Q9VTC4	9.4e-35	50.00	MIP18 family protein galla-2 OS=Drosophila melanogaster OX=7227 GN=galla-2 PE=1 ...	[more]
Q9D187	4.7e-34	48.65	Cytosolic iron-sulfur assembly component 2B OS=Mus musculus OX=10090 GN=Ciao2b P...	[more]

BLAST of Cucsat.G12022.T2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004149152.1	2.21e-105	100.00	protein AE7-like 1 [Cucumis sativus] >XP_008453741.1 PREDICTED: protein AE7-like...	[more]
XP_022136890.1	1.75e-102	97.40	protein AE7-like 1 [Momordica charantia]	[more]
XP_022984190.1	1.44e-101	97.39	protein AE7-like 1 isoform X2 [Cucurbita maxima]	[more]
XP_015889928.1	2.04e-101	94.81	protein AE7-like 1 [Ziziphus jujuba]	[more]
XP_023551936.1	2.04e-101	97.39	protein AE7-like 1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cucsat.G12022.T2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KUM2	1.07e-105	100.00	FeS_assembly_P domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G038...	[more]
A0A1S3BWZ8	1.07e-105	100.00	protein AE7-like 1 OS=Cucumis melo OX=3656 GN=LOC103494387 PE=3 SV=1	[more]
A0A6J1C573	8.45e-103	97.40	protein AE7-like 1 OS=Momordica charantia OX=3673 GN=LOC111008470 PE=3 SV=1	[more]
A0A6J1J802	6.95e-102	97.39	protein AE7-like 1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111482581 PE=3 S...	[more]
A0A6P4A8B7	9.87e-102	94.81	protein AE7-like 1 OS=Ziziphus jujuba OX=326968 GN=LOC107424594 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002744	MIP18 family-like	PFAM	PF01883	FeS_assembly_P	coord: 35..103 e-value: 1.0E-8 score: 35.3
None	No IPR available	GENE3D	6.10.250.1280		coord: 118..149 e-value: 1.6E-18 score: 68.1
None	No IPR available	PANTHER	PTHR12377:SF3	PROTEIN AE7-LIKE 1	coord: 1..153
IPR034904	Fe-S cluster assembly domain superfamily	GENE3D	3.30.300.130		coord: 28..117 e-value: 6.1E-34 score: 117.7
IPR034904	Fe-S cluster assembly domain superfamily	SUPERFAMILY	117916	Fe-S cluster assembly (FSCA) domain-like	coord: 35..125
IPR039796	MIP18 family	PANTHER	PTHR12377	UNCHARACTERIZED	coord: 1..153

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G12022	Cucsat.G12022	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G12022.T2.E1	Cucsat.G12022.T2.E1	exon
Cucsat.G12022.T2.E2	Cucsat.G12022.T2.E2	exon
Cucsat.G12022.T2.E3	Cucsat.G12022.T2.E3	exon
Cucsat.G12022.T2.E4	Cucsat.G12022.T2.E4	exon
Cucsat.G12022.T2.E5	Cucsat.G12022.T2.E5	exon
Cucsat.G12022.T2.E6	Cucsat.G12022.T2.E6	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G12022.T2.C5	Cucsat.G12022.T2.C5	CDS
Cucsat.G12022.T2.C4	Cucsat.G12022.T2.C4	CDS
Cucsat.G12022.T2.C3	Cucsat.G12022.T2.C3	CDS
Cucsat.G12022.T2.C2	Cucsat.G12022.T2.C2	CDS
Cucsat.G12022.T2.C1	Cucsat.G12022.T2.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G12022.T2	Cucsat.G12022.T2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0106035	protein maturation by [4Fe-4S] cluster transfer
biological_process	GO:0016226	iron-sulfur cluster assembly
cellular_component	GO:0016021	integral component of membrane