Cucsat.G12006.T2 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G12006.T2
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Epoxide hydrolase
Location	ctg1820: 4847415 .. 4849087 (-)
RNA-Seq Expression	Cucsat.G12006.T2
Synteny	Cucsat.G12006.T2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCGTTCCTCCAAAGTCATATTCCAAATTCAACTCCCACTTCTAATTAGGATCACCATGGTGAACTTGGTTGCAGCTCAACGCCCACTCTTACATGCCCTAATGAAGATGGCCGGTGTCGTACCTTACTCGGTCGACATCGAGCCCGGCACCATCATGAACTTCTGGGTCCCTATCGAGTCTCTCCCTAAAACCAAAAAAGGCCAACCCCCGAAACCTCCGCAAAAACCAACCAAGCCTGTTGTTATCTTGATTCATGGTTTTGCAGCTGAAGGTATTGTCACTTGGCAATTTCAGGTATTAACTACACAAATTCTAACATACAACTATAATTAAAATGAGTTACAATTATCTTTGATTCAAAACAATTTAAATTCCTATCACATAAATTAGTATTAAGATAAATTAATAGATAAACTAATTAGTGACTGTATATTTTAAAAAGAATACATAGCCCACATCATAGAAAAAGGAATATTCATATCTAAATTCAATGAAATTCTTTGAAGTTAGACAAAAAGATGATACGAAATTGAAAGTTGAATAGAGAACTATAGATAGAGATCAAAAGTGTTTTTTTGTTAACCAATTTTTTCAATTTTGGATGTGCTTATTTAGGTGGGTGCTCTTTCAAAACAATACTCTGTTTACGTGCCCGACCTTCTATTTTTTGGCGACTCCATCACTGATAAAACAGAGCGCTCACCGGCGTTCCAAGCGGAGTGTTTGGCAATAGGGCTGAGGAAGCTCGGAATTGAGAAATGTTCGGTGGTGGGGTTTAGTTACGGCGGGATGGTGGCGTTCAAATTGGTGGAGCTCCGACCAGAACTGGTGGATGCCATGGTGGTTTCTGGCTCGATTTTGGCTATGACAGATTCAATCAGCGACGAGACCCTGCACAGATTAGGGTTTAGGTCCTCCTCGGATCTCTTACTTCCGACGTCTGTCAAGGGTCTGAAAGCTCTTCTCTCAGTCGCTGCTCATAAAAAACTCTGGTTTCCCGATCGACTCCATAAAGACTTCCTGGAGGTCAGTACGAATACTCTGAGCTATATGTATGTATGTATATATAACACATAAAGACTGGTAATTGGTTCTCATCATAGAAAATACTATTTTTACTGAAATTCAATACTCCTGCGACATGACAGAATGAAATTGATGTATATAGAACTTTAAACTAATATATTGAAGTGATGATTGAAATTAGAGGGGACTAGCTACGTGATGAACCTAACAGTTTTTTACGTAATTGGCTATTGTTTACTCTACAAAATTTTGAAATTGAATTGCAGGGGATGTTCCTGTCATTATTTACGTTTGATTTATTTATTTTTGCCCATGATGAACAATTGTAGGTGATGTTCAATAACAGGAAAGATAGAGCAGAGCTCCTTCAAGGCTTAGTTATCAGCAACAAAGACACCATAGTTCCGAAGTTTACTCAGGCAAATATTTCACACCTCTGTCTTTCTTAATTGTGTGCTTCCATTTTCTTTCAGTTTAAGAATGCGCTAATGATTCTTCATCTCTCTCTCTTTCCTCTGCAGAAGATACATCTTTTATGGGGCGAAAACGATCAGATTTTCAATGTAGACCTCGCCCGAAAACTACAACAGTATGCATGATTTTCATTATTTGAAATTGGAATCCAGAGTTTGTTTCAGTTCTAAA

Coding sequence (CDS)

CGTTCCTCCAAAGTCATATTCCAAATTCAACTCCCACTTCTAATTAGGATCACCATGGTGAACTTGGTTGCAGCTCAACGCCCACTCTTACATGCCCTAATGAAGATGGCCGGTGTCGTACCTTACTCGGTCGACATCGAGCCCGGCACCATCATGAACTTCTGGGTCCCTATCGAGTCTCTCCCTAAAACCAAAAAAGGCCAACCCCCGAAACCTCCGCAAAAACCAACCAAGCCTGTTGTTATCTTGATTCATGGTTTTGCAGCTGAAGGTATTGTCACTTGGCAATTTCAGGTGGGTGCTCTTTCAAAACAATACTCTGTTTACGTGCCCGACCTTCTATTTTTTGGCGACTCCATCACTGATAAAACAGAGCGCTCACCGGCGTTCCAAGCGGAGTGTTTGGCAATAGGGCTGAGGAAGCTCGGAATTGAGAAATGTTCGGTGGTGGGGTTTAGTTACGGCGGGATGGTGGCGTTCAAATTGGTGGAGCTCCGACCAGAACTGGTGGATGCCATGGTGGTTTCTGGCTCGATTTTGGCTATGACAGATTCAATCAGCGACGAGACCCTGCACAGATTAGGGTTTAGGTCCTCCTCGGATCTCTTACTTCCGACGTCTGTCAAGGGTCTGAAAGCTCTTCTCTCAGTCGCTGCTCATAAAAAACTCTGGTTTCCCGATCGACTCCATAAAGACTTCCTGGAGGTGATGTTCAATAACAGGAAAGATAGAGCAGAGCTCCTTCAAGGCTTAGTTATCAGCAACAAAGACACCATAGTTCCGAAGTTTACTCAGAAGATACATCTTTTATGGGGCGAAAACGATCAGATTTTCAATGTAGACCTCGCCCGAAAACTACAACAGTATGCATGA

Protein sequence

RSSKVIFQIQLPLLIRITMVNLVAAQRPLLHALMKMAGVVPYSVDIEPGTIMNFWVPIESLPKTKKGQPPKPPQKPTKPVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAECLAIGLRKLGIEKCSVVGFSYGGMVAFKLVELRPELVDAMVVSGSILAMTDSISDETLHRLGFRSSSDLLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLVISNKDTIVPKFTQKIHLLWGENDQIFNVDLARKLQQYA

Homology

[Close]

BLAST of Cucsat.G12006.T2 vs. ExPASy Swiss-Prot
Match: Q6IE26 (Epoxide hydrolase 4 OS=Mus musculus OX=10090 GN=Ephx4 PE=2 SV=2)

HSP 1 Score: 60.1 bits (144), Expect = 4.9e-08
Identity = 30/102 (29.41%), Postives = 56/102 (54.90%), Query Frame = 0


Query: 78  KPVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAECLAI 137
           KP+++L+HGF  E   +W+ Q+     +Y V   DL  +G+S  D      +++ +CL  
Sbjct: 91  KPLMLLLHGF-PEFWYSWRHQLREFKSEYRVVALDLRGYGES--DAPAHQESYKLDCLIA 150

Query: 138 GLR----KLGIEKCSVVGFSYGGMVAFKLVELRPELVDAMVV 176
            ++     LG  KC ++G  +GGM+A+ +    PE++  ++V
Sbjct: 151 DIKDILDSLGYSKCVLIGHDWGGMIAWLIAVCYPEMIMKLIV 189

[Close]

BLAST of Cucsat.G12006.T2 vs. ExPASy Swiss-Prot
Match: Q8IUS5 (Epoxide hydrolase 4 OS=Homo sapiens OX=9606 GN=EPHX4 PE=1 SV=2)

HSP 1 Score: 59.7 bits (143), Expect = 6.3e-08
Identity = 31/102 (30.39%), Postives = 56/102 (54.90%), Query Frame = 0


Query: 78  KPVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAECLAI 137
           KP+++L+HGF  E   +W++Q+     +Y V   DL  +G+  TD       ++ +CL  
Sbjct: 93  KPLMLLLHGF-PEFWYSWRYQLREFKSEYRVVALDLRGYGE--TDAPIHRQNYKLDCLIT 152

Query: 138 GLR----KLGIEKCSVVGFSYGGMVAFKLVELRPELVDAMVV 176
            ++     LG  KC ++G  +GGM+A+ +    PE+V  ++V
Sbjct: 153 DIKDILDSLGYSKCVLIGHDWGGMIAWLIAICYPEMVMKLIV 191

[Close]

BLAST of Cucsat.G12006.T2 vs. ExPASy Swiss-Prot
Match: P9WNH2 (Uncharacterized protein MT2788 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=MT2788 PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.1e-07
Identity = 33/97 (34.02%), Postives = 52/97 (53.61%), Query Frame = 0


Query: 79  PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAECLAIG 138
           P ++LIHG   +   TW      L+++++V  PDLL  G S   + + S A  A  +   
Sbjct: 38  PAILLIHGI-GDNSTTWNGVHAKLAQRFTVIAPDLLGHGQSDKPRADYSVAAYANGMRDL 97

Query: 139 LRKLGIEKCSVVGFSYGGMVAFKLVELRPELVDAMVV 176
           L  L IE+ ++VG S GG VA +     P+LVD +++
Sbjct: 98  LSVLDIERVTIVGHSLGGGVAMQFAYQFPQLVDRLIL 133

[Close]

BLAST of Cucsat.G12006.T2 vs. ExPASy Swiss-Prot
Match: P9WNH3 (Uncharacterized protein Rv2715 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=Rv2715 PE=1 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.1e-07
Identity = 33/97 (34.02%), Postives = 52/97 (53.61%), Query Frame = 0


Query: 79  PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAECLAIG 138
           P ++LIHG   +   TW      L+++++V  PDLL  G S   + + S A  A  +   
Sbjct: 38  PAILLIHGI-GDNSTTWNGVHAKLAQRFTVIAPDLLGHGQSDKPRADYSVAAYANGMRDL 97

Query: 139 LRKLGIEKCSVVGFSYGGMVAFKLVELRPELVDAMVV 176
           L  L IE+ ++VG S GG VA +     P+LVD +++
Sbjct: 98  LSVLDIERVTIVGHSLGGGVAMQFAYQFPQLVDRLIL 133

[Close]

BLAST of Cucsat.G12006.T2 vs. ExPASy Swiss-Prot
Match: P0A573 (Uncharacterized protein Mb2734 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=BQ2027_MB2734 PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.1e-07
Identity = 33/97 (34.02%), Postives = 52/97 (53.61%), Query Frame = 0


Query: 79  PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAECLAIG 138
           P ++LIHG   +   TW      L+++++V  PDLL  G S   + + S A  A  +   
Sbjct: 38  PAILLIHGI-GDNSTTWNGVHAKLAQRFTVIAPDLLGHGQSDKPRADYSVAAYANGMRDL 97

Query: 139 LRKLGIEKCSVVGFSYGGMVAFKLVELRPELVDAMVV 176
           L  L IE+ ++VG S GG VA +     P+LVD +++
Sbjct: 98  LSVLDIERVTIVGHSLGGGVAMQFAYQFPQLVDRLIL 133

[Close]

BLAST of Cucsat.G12006.T2 vs. NCBI nr
Match: XP_004146500.2 (uncharacterized protein LOC101205115 [Cucumis sativus] >KGN53303.1 hypothetical protein Csa_014480 [Cucumis sativus])

HSP 1 Score: 538 bits (1386), Expect = 8.40e-192
Identity = 270/270 (100.00%), Postives = 270/270 (100.00%), Query Frame = 0


Query: 19  MVNLVAAQRPLLHALMKMAGVVPYSVDIEPGTIMNFWVPIESLPKTKKGQPPKPPQKPTK 78
           MVNLVAAQRPLLHALMKMAGVVPYSVDIEPGTIMNFWVPIESLPKTKKGQPPKPPQKPTK
Sbjct: 1   MVNLVAAQRPLLHALMKMAGVVPYSVDIEPGTIMNFWVPIESLPKTKKGQPPKPPQKPTK 60

Query: 79  PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAECLAIG 138
           PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAECLAIG
Sbjct: 61  PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAECLAIG 120

Query: 139 LRKLGIEKCSVVGFSYGGMVAFKLVELRPELVDAMVVSGSILAMTDSISDETLHRLGFRS 198
           LRKLGIEKCSVVGFSYGGMVAFKLVELRPELVDAMVVSGSILAMTDSISDETLHRLGFRS
Sbjct: 121 LRKLGIEKCSVVGFSYGGMVAFKLVELRPELVDAMVVSGSILAMTDSISDETLHRLGFRS 180

Query: 199 SSDLLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLVISNKDT 258
           SSDLLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLVISNKDT
Sbjct: 181 SSDLLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLVISNKDT 240

Query: 259 IVPKFTQKIHLLWGENDQIFNVDLARKLQQ 288
           IVPKFTQKIHLLWGENDQIFNVDLARKLQQ
Sbjct: 241 IVPKFTQKIHLLWGENDQIFNVDLARKLQQ 270

[Close]

BLAST of Cucsat.G12006.T2 vs. NCBI nr
Match: XP_008452086.1 (PREDICTED: uncharacterized protein LOC103493198 isoform X2 [Cucumis melo])

HSP 1 Score: 520 bits (1339), Expect = 2.61e-185
Identity = 257/272 (94.49%), Postives = 268/272 (98.53%), Query Frame = 0


Query: 19  MVNLVAAQRPLLHALMKMAGVVPYSVDIEPGTIMNFWVPIESLPKTKKGQPPKPPQKPTK 78
           MVNLVAAQRPLLHALMKMAGV+PYSVDIEPGTIMNFWVPI++LPKTKK QPPK PQKPTK
Sbjct: 1   MVNLVAAQRPLLHALMKMAGVIPYSVDIEPGTIMNFWVPIDTLPKTKKDQPPKTPQKPTK 60

Query: 79  PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAECLAIG 138
           PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQA+CLAIG
Sbjct: 61  PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAQCLAIG 120

Query: 139 LRKLGIEKCSVVGFSYGGMVAFKLVELRPELVDAMVVSGSILAMTDSISDETLHRLGFRS 198
           LRKLGIE+CSVVGFSYGGMVAFKLVEL+PELVDAMVVSGSILAMTDSISD+TL RLGF+S
Sbjct: 121 LRKLGIERCSVVGFSYGGMVAFKLVELQPELVDAMVVSGSILAMTDSISDDTLQRLGFKS 180

Query: 199 SSDLLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLVISNKDT 258
           SSDLLLPTSVKGLKALLSVAAH+KLWFPDRLHKDFLEVMFNNRKDRAELLQGL+ISNKDT
Sbjct: 181 SSDLLLPTSVKGLKALLSVAAHRKLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT 240

Query: 259 IVPKFTQKIHLLWGENDQIFNVDLARKLQQYA 290
            VPKFTQKIHLLWGENDQIFNV+LARKLQQYA
Sbjct: 241 TVPKFTQKIHLLWGENDQIFNVELARKLQQYA 272

[Close]

BLAST of Cucsat.G12006.T2 vs. NCBI nr
Match: XP_008452085.1 (PREDICTED: putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase isoform X1 [Cucumis melo] >TYK16669.1 putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 515 bits (1327), Expect = 8.19e-183
Identity = 255/270 (94.44%), Postives = 266/270 (98.52%), Query Frame = 0


Query: 19  MVNLVAAQRPLLHALMKMAGVVPYSVDIEPGTIMNFWVPIESLPKTKKGQPPKPPQKPTK 78
           MVNLVAAQRPLLHALMKMAGV+PYSVDIEPGTIMNFWVPI++LPKTKK QPPK PQKPTK
Sbjct: 1   MVNLVAAQRPLLHALMKMAGVIPYSVDIEPGTIMNFWVPIDTLPKTKKDQPPKTPQKPTK 60

Query: 79  PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAECLAIG 138
           PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQA+CLAIG
Sbjct: 61  PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAQCLAIG 120

Query: 139 LRKLGIEKCSVVGFSYGGMVAFKLVELRPELVDAMVVSGSILAMTDSISDETLHRLGFRS 198
           LRKLGIE+CSVVGFSYGGMVAFKLVEL+PELVDAMVVSGSILAMTDSISD+TL RLGF+S
Sbjct: 121 LRKLGIERCSVVGFSYGGMVAFKLVELQPELVDAMVVSGSILAMTDSISDDTLQRLGFKS 180

Query: 199 SSDLLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLVISNKDT 258
           SSDLLLPTSVKGLKALLSVAAH+KLWFPDRLHKDFLEVMFNNRKDRAELLQGL+ISNKDT
Sbjct: 181 SSDLLLPTSVKGLKALLSVAAHRKLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT 240

Query: 259 IVPKFTQKIHLLWGENDQIFNVDLARKLQQ 288
            VPKFTQKIHLLWGENDQIFNV+LARKLQQ
Sbjct: 241 TVPKFTQKIHLLWGENDQIFNVELARKLQQ 270

[Close]

BLAST of Cucsat.G12006.T2 vs. NCBI nr
Match: XP_038904799.1 (monoacylglycerol lipase ABHD6-like [Benincasa hispida])

HSP 1 Score: 498 bits (1282), Expect = 5.86e-176
Identity = 244/270 (90.37%), Postives = 259/270 (95.93%), Query Frame = 0


Query: 19  MVNLVAAQRPLLHALMKMAGVVPYSVDIEPGTIMNFWVPIESLPKTKKGQPPKPPQKPTK 78
           MVNLVAAQRPLLHALMKMAGVVP+SVDIEPGT+MNFWVP+E+LP TKK QPPKPP+KP K
Sbjct: 1   MVNLVAAQRPLLHALMKMAGVVPFSVDIEPGTVMNFWVPLEALPTTKKDQPPKPPKKPKK 60

Query: 79  PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAECLAIG 138
           PVV+LIHGFAAEGIVTWQFQVGALSKQYSVYVPDL+FFG S+TDK ERSPAFQAECLA G
Sbjct: 61  PVVVLIHGFAAEGIVTWQFQVGALSKQYSVYVPDLIFFGGSVTDKAERSPAFQAECLATG 120

Query: 139 LRKLGIEKCSVVGFSYGGMVAFKLVELRPELVDAMVVSGSILAMTDSISDETLHRLGFRS 198
           LRKLGIEKC+VVGFSYGGMVAFKL ELRPELVDAMVVSGSILAMTDSIS ETL RLGF S
Sbjct: 121 LRKLGIEKCTVVGFSYGGMVAFKLAELRPELVDAMVVSGSILAMTDSISGETLQRLGFES 180

Query: 199 SSDLLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLVISNKDT 258
           SS+LLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGL+ISNKDT
Sbjct: 181 SSELLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT 240

Query: 259 IVPKFTQKIHLLWGENDQIFNVDLARKLQQ 288
           +VPKFTQKIHLLWGENDQIF V+LA+KLQ+
Sbjct: 241 VVPKFTQKIHLLWGENDQIFKVELAQKLQE 270

[Close]

BLAST of Cucsat.G12006.T2 vs. NCBI nr
Match: KAA0044794.1 (putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 496 bits (1277), Expect = 4.52e-175
Identity = 250/278 (89.93%), Postives = 261/278 (93.88%), Query Frame = 0


Query: 19  MVNLVAAQRPLLHALMKMAGVVPYSVDIEPGTIMNFWVPIESLPKTKKGQPPKPPQKPTK 78
           MVNLVAAQRPLLHALMKMAGV+PYSVDIEPGTIMNFWVPI++LPKTKK QPPK PQKPTK
Sbjct: 1   MVNLVAAQRPLLHALMKMAGVIPYSVDIEPGTIMNFWVPIDTLPKTKKDQPPKTPQKPTK 60

Query: 79  PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAECLAIG 138
           PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQA+CLAIG
Sbjct: 61  PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAQCLAIG 120

Query: 139 LRKLGIEKCSVVGFSYGGMVAFKLVELRPELVDAMVVSGSILAMTDSISDETLHRLGFRS 198
           LRKLGIE+CSVVGFSYGGMVAFKLVEL+PELVDAMVVSGSILAMTDSISD+TL RLGF+S
Sbjct: 121 LRKLGIERCSVVGFSYGGMVAFKLVELQPELVDAMVVSGSILAMTDSISDDTLQRLGFKS 180

Query: 199 SSDLLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLVISNKDT 258
           SSDLLLPTSVKGLKALLSVAAH+KLWFPDRLHKDFLEVMFNNRKDRAELLQGL+ISNKDT
Sbjct: 181 SSDLLLPTSVKGLKALLSVAAHRKLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT 240

Query: 259 IVPKFT--------QKIHLLWGENDQIFNVDLARKLQQ 288
                         QKIHLLWGENDQIFNV+LARKLQQ
Sbjct: 241 TTALMIFHLSLSPLQKIHLLWGENDQIFNVELARKLQQ 278

[Close]

BLAST of Cucsat.G12006.T2 vs. ExPASy TrEMBL
Match: A0A0A0KUS2 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G046660 PE=4 SV=1)

HSP 1 Score: 538 bits (1386), Expect = 4.07e-192
Identity = 270/270 (100.00%), Postives = 270/270 (100.00%), Query Frame = 0


Query: 19  MVNLVAAQRPLLHALMKMAGVVPYSVDIEPGTIMNFWVPIESLPKTKKGQPPKPPQKPTK 78
           MVNLVAAQRPLLHALMKMAGVVPYSVDIEPGTIMNFWVPIESLPKTKKGQPPKPPQKPTK
Sbjct: 1   MVNLVAAQRPLLHALMKMAGVVPYSVDIEPGTIMNFWVPIESLPKTKKGQPPKPPQKPTK 60

Query: 79  PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAECLAIG 138
           PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAECLAIG
Sbjct: 61  PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAECLAIG 120

Query: 139 LRKLGIEKCSVVGFSYGGMVAFKLVELRPELVDAMVVSGSILAMTDSISDETLHRLGFRS 198
           LRKLGIEKCSVVGFSYGGMVAFKLVELRPELVDAMVVSGSILAMTDSISDETLHRLGFRS
Sbjct: 121 LRKLGIEKCSVVGFSYGGMVAFKLVELRPELVDAMVVSGSILAMTDSISDETLHRLGFRS 180

Query: 199 SSDLLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLVISNKDT 258
           SSDLLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLVISNKDT
Sbjct: 181 SSDLLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLVISNKDT 240

Query: 259 IVPKFTQKIHLLWGENDQIFNVDLARKLQQ 288
           IVPKFTQKIHLLWGENDQIFNVDLARKLQQ
Sbjct: 241 IVPKFTQKIHLLWGENDQIFNVDLARKLQQ 270

[Close]

BLAST of Cucsat.G12006.T2 vs. ExPASy TrEMBL
Match: A0A1S3BU64 (uncharacterized protein LOC103493198 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103493198 PE=4 SV=1)

HSP 1 Score: 520 bits (1339), Expect = 1.26e-185
Identity = 257/272 (94.49%), Postives = 268/272 (98.53%), Query Frame = 0


Query: 19  MVNLVAAQRPLLHALMKMAGVVPYSVDIEPGTIMNFWVPIESLPKTKKGQPPKPPQKPTK 78
           MVNLVAAQRPLLHALMKMAGV+PYSVDIEPGTIMNFWVPI++LPKTKK QPPK PQKPTK
Sbjct: 1   MVNLVAAQRPLLHALMKMAGVIPYSVDIEPGTIMNFWVPIDTLPKTKKDQPPKTPQKPTK 60

Query: 79  PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAECLAIG 138
           PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQA+CLAIG
Sbjct: 61  PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAQCLAIG 120

Query: 139 LRKLGIEKCSVVGFSYGGMVAFKLVELRPELVDAMVVSGSILAMTDSISDETLHRLGFRS 198
           LRKLGIE+CSVVGFSYGGMVAFKLVEL+PELVDAMVVSGSILAMTDSISD+TL RLGF+S
Sbjct: 121 LRKLGIERCSVVGFSYGGMVAFKLVELQPELVDAMVVSGSILAMTDSISDDTLQRLGFKS 180

Query: 199 SSDLLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLVISNKDT 258
           SSDLLLPTSVKGLKALLSVAAH+KLWFPDRLHKDFLEVMFNNRKDRAELLQGL+ISNKDT
Sbjct: 181 SSDLLLPTSVKGLKALLSVAAHRKLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT 240

Query: 259 IVPKFTQKIHLLWGENDQIFNVDLARKLQQYA 290
            VPKFTQKIHLLWGENDQIFNV+LARKLQQYA
Sbjct: 241 TVPKFTQKIHLLWGENDQIFNVELARKLQQYA 272

[Close]

BLAST of Cucsat.G12006.T2 vs. ExPASy TrEMBL
Match: A0A5D3D2C9 (Putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G005000 PE=4 SV=1)

HSP 1 Score: 515 bits (1327), Expect = 3.97e-183
Identity = 255/270 (94.44%), Postives = 266/270 (98.52%), Query Frame = 0


Query: 19  MVNLVAAQRPLLHALMKMAGVVPYSVDIEPGTIMNFWVPIESLPKTKKGQPPKPPQKPTK 78
           MVNLVAAQRPLLHALMKMAGV+PYSVDIEPGTIMNFWVPI++LPKTKK QPPK PQKPTK
Sbjct: 1   MVNLVAAQRPLLHALMKMAGVIPYSVDIEPGTIMNFWVPIDTLPKTKKDQPPKTPQKPTK 60

Query: 79  PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAECLAIG 138
           PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQA+CLAIG
Sbjct: 61  PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAQCLAIG 120

Query: 139 LRKLGIEKCSVVGFSYGGMVAFKLVELRPELVDAMVVSGSILAMTDSISDETLHRLGFRS 198
           LRKLGIE+CSVVGFSYGGMVAFKLVEL+PELVDAMVVSGSILAMTDSISD+TL RLGF+S
Sbjct: 121 LRKLGIERCSVVGFSYGGMVAFKLVELQPELVDAMVVSGSILAMTDSISDDTLQRLGFKS 180

Query: 199 SSDLLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLVISNKDT 258
           SSDLLLPTSVKGLKALLSVAAH+KLWFPDRLHKDFLEVMFNNRKDRAELLQGL+ISNKDT
Sbjct: 181 SSDLLLPTSVKGLKALLSVAAHRKLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT 240

Query: 259 IVPKFTQKIHLLWGENDQIFNVDLARKLQQ 288
            VPKFTQKIHLLWGENDQIFNV+LARKLQQ
Sbjct: 241 TVPKFTQKIHLLWGENDQIFNVELARKLQQ 270

[Close]

BLAST of Cucsat.G12006.T2 vs. ExPASy TrEMBL
Match: A0A1S3BTU7 (putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493198 PE=4 SV=1)

HSP 1 Score: 515 bits (1327), Expect = 3.97e-183
Identity = 255/270 (94.44%), Postives = 266/270 (98.52%), Query Frame = 0


Query: 19  MVNLVAAQRPLLHALMKMAGVVPYSVDIEPGTIMNFWVPIESLPKTKKGQPPKPPQKPTK 78
           MVNLVAAQRPLLHALMKMAGV+PYSVDIEPGTIMNFWVPI++LPKTKK QPPK PQKPTK
Sbjct: 1   MVNLVAAQRPLLHALMKMAGVIPYSVDIEPGTIMNFWVPIDTLPKTKKDQPPKTPQKPTK 60

Query: 79  PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAECLAIG 138
           PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQA+CLAIG
Sbjct: 61  PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAQCLAIG 120

Query: 139 LRKLGIEKCSVVGFSYGGMVAFKLVELRPELVDAMVVSGSILAMTDSISDETLHRLGFRS 198
           LRKLGIE+CSVVGFSYGGMVAFKLVEL+PELVDAMVVSGSILAMTDSISD+TL RLGF+S
Sbjct: 121 LRKLGIERCSVVGFSYGGMVAFKLVELQPELVDAMVVSGSILAMTDSISDDTLQRLGFKS 180

Query: 199 SSDLLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLVISNKDT 258
           SSDLLLPTSVKGLKALLSVAAH+KLWFPDRLHKDFLEVMFNNRKDRAELLQGL+ISNKDT
Sbjct: 181 SSDLLLPTSVKGLKALLSVAAHRKLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT 240

Query: 259 IVPKFTQKIHLLWGENDQIFNVDLARKLQQ 288
            VPKFTQKIHLLWGENDQIFNV+LARKLQQ
Sbjct: 241 TVPKFTQKIHLLWGENDQIFNVELARKLQQ 270

[Close]

BLAST of Cucsat.G12006.T2 vs. ExPASy TrEMBL
Match: A0A5A7TNT7 (Putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G00850 PE=4 SV=1)

HSP 1 Score: 496 bits (1277), Expect = 2.19e-175
Identity = 250/278 (89.93%), Postives = 261/278 (93.88%), Query Frame = 0


Query: 19  MVNLVAAQRPLLHALMKMAGVVPYSVDIEPGTIMNFWVPIESLPKTKKGQPPKPPQKPTK 78
           MVNLVAAQRPLLHALMKMAGV+PYSVDIEPGTIMNFWVPI++LPKTKK QPPK PQKPTK
Sbjct: 1   MVNLVAAQRPLLHALMKMAGVIPYSVDIEPGTIMNFWVPIDTLPKTKKDQPPKTPQKPTK 60

Query: 79  PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAECLAIG 138
           PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQA+CLAIG
Sbjct: 61  PVVILIHGFAAEGIVTWQFQVGALSKQYSVYVPDLLFFGDSITDKTERSPAFQAQCLAIG 120

Query: 139 LRKLGIEKCSVVGFSYGGMVAFKLVELRPELVDAMVVSGSILAMTDSISDETLHRLGFRS 198
           LRKLGIE+CSVVGFSYGGMVAFKLVEL+PELVDAMVVSGSILAMTDSISD+TL RLGF+S
Sbjct: 121 LRKLGIERCSVVGFSYGGMVAFKLVELQPELVDAMVVSGSILAMTDSISDDTLQRLGFKS 180

Query: 199 SSDLLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLEVMFNNRKDRAELLQGLVISNKDT 258
           SSDLLLPTSVKGLKALLSVAAH+KLWFPDRLHKDFLEVMFNNRKDRAELLQGL+ISNKDT
Sbjct: 181 SSDLLLPTSVKGLKALLSVAAHRKLWFPDRLHKDFLEVMFNNRKDRAELLQGLIISNKDT 240

Query: 259 IVPKFT--------QKIHLLWGENDQIFNVDLARKLQQ 288
                         QKIHLLWGENDQIFNV+LARKLQQ
Sbjct: 241 TTALMIFHLSLSPLQKIHLLWGENDQIFNVELARKLQQ 278

The following BLAST results are available for this feature:

BLAST of Cucsat.G12006.T2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6IE26	4.9e-08	29.41	Epoxide hydrolase 4 OS=Mus musculus OX=10090 GN=Ephx4 PE=2 SV=2	[more]
Q8IUS5	6.3e-08	30.39	Epoxide hydrolase 4 OS=Homo sapiens OX=9606 GN=EPHX4 PE=1 SV=2	[more]
P9WNH2	1.1e-07	34.02	Uncharacterized protein MT2788 OS=Mycobacterium tuberculosis (strain CDC 1551 / ...	[more]
P9WNH3	1.1e-07	34.02	Uncharacterized protein Rv2715 OS=Mycobacterium tuberculosis (strain ATCC 25618 ...	[more]
P0A573	1.1e-07	34.02	Uncharacterized protein Mb2734 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2...	[more]

BLAST of Cucsat.G12006.T2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004146500.2	8.40e-192	100.00	uncharacterized protein LOC101205115 [Cucumis sativus] >KGN53303.1 hypothetical ...	[more]
XP_008452086.1	2.61e-185	94.49	PREDICTED: uncharacterized protein LOC103493198 isoform X2 [Cucumis melo]	[more]
XP_008452085.1	8.19e-183	94.44	PREDICTED: putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate syntha...	[more]
XP_038904799.1	5.86e-176	90.37	monoacylglycerol lipase ABHD6-like [Benincasa hispida]	[more]
KAA0044794.1	4.52e-175	89.93	putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase isoform ...	[more]

BLAST of Cucsat.G12006.T2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KUS2	4.07e-192	100.00	AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G046...	[more]
A0A1S3BU64	1.26e-185	94.49	uncharacterized protein LOC103493198 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A5D3D2C9	3.97e-183	94.44	Putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase isoform ...	[more]
A0A1S3BTU7	3.97e-183	94.44	putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase isoform ...	[more]
A0A5A7TNT7	2.19e-175	89.93	Putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase isoform ...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000073	Alpha/beta hydrolase fold-1	PFAM	PF00561	Abhydrolase_1	coord: 79..185 e-value: 5.3E-16 score: 59.0
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 71..290 e-value: 8.0E-33 score: 116.1
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 77..289
None	No IPR available	PANTHER	PTHR43139	SI:DKEY-122A22.2	coord: 20..287
None	No IPR available	PANTHER	PTHR43139:SF53	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 20..287

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G12006	Cucsat.G12006	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G12006.T2.E1	Cucsat.G12006.T2.E1	exon
Cucsat.G12006.T2.E2	Cucsat.G12006.T2.E2	exon
Cucsat.G12006.T2.E3	Cucsat.G12006.T2.E3	exon
Cucsat.G12006.T2.E4	Cucsat.G12006.T2.E4	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G12006.T2.C4	Cucsat.G12006.T2.C4	CDS
Cucsat.G12006.T2.C3	Cucsat.G12006.T2.C3	CDS
Cucsat.G12006.T2.C2	Cucsat.G12006.T2.C2	CDS
Cucsat.G12006.T2.C1	Cucsat.G12006.T2.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G12006.T2	Cucsat.G12006.T2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016787	hydrolase activity