Cucsat.G11973.T10 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G11973.T10
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Endoglucanase
Location	ctg1820: 4176337 .. 4181542 (-)
RNA-Seq Expression	Cucsat.G11973.T10
Synteny	Cucsat.G11973.T10

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCTCCAAGCTTATATAGTGCTCCCATCCTCACTTCCACTCCCCACCCCACACTCTCCTTCTTGTTCATTCTTCATTTCTCTCTTCCCCTCTTTAGGGGTTCTTCTCCTCTGTTTCATAATCGATTTATCACAACTTTTATCTTGATAAATCCCACCGGCATTGCCATTTCCTTCTCCCATTCCTTCAAAATGGCAAATCCCATTTCCCCCATTGCTCTCTTTATCCTACTTCTAGCCCCCTCTGCCCTAGCCGGCCATGATTACGGCCAAGCTCTCACCAAGAGCCTTCTCTTCTTCGAAGCTCAGAGATCCGGCTACCTCCCCCACAACCAGCGCGTCAATTGGCGAGCCAATTCCGGCCTCCAAGACGGCAAGGCTAGCGGGGTAAACCCTTTAAAACCCTGTTTGGTTACTCAGAAAATCGATCGGGAAACTTAATTTGGGGATTTTGTATGTTTGTTGTAGGTGGATCTGGTCGGAGGATACTACGACGCCGGTGACAATGTGAAGTTCGGACTTCCGATGGCGTTCACGGTGACGATGATGAGTTGGAGTATTATTGAATATGGAAAACAAATGGCCACTAATGGCGAACTTAGCCATGCCCTAGACGCCGTCAAATGGGGCACCGATTACTTCATCAAAGCCCACCCTGAACCCTATGTTCTCTATGGAGAGGTAACCTTAATCTTTTTCGTTCTTTTTAATTCAACGATTTCAAATTAATCCAACCATCAAACTTTGATATGATAAGTGGGTCAATTCAACGCAATTTTCTTTCATAACGTTAGATTTTATTAGGTAGAGATGTTTTAATTTGTACGTTGATTTGTTATGAAATGATAGTTCTATTAATGTTTTACTGACCATTATGCTTAACTAGTTAGAATAAAATTGGTAATTATACTTAAGGGAAGGACTCTAATATCTAATTTTTTTACTGATATTTGACTATTTTAGTAAATTAAACACCAATTTGTCGCCTTGTTCAAATGTCTTATGTTAGTTGTTAAATTTATTATTAAAATATAAGTGTGTGTATTTTAGGGGTGATTTGTCAATTGCATAAATGAGAGGGACTTAATTTGTAATTAGGCTTGATTTGTGTGTGTGTGTTTTTTTAGTTAAAATTATGAAAAAGGGATTAACAACATGGTTAATGAAATGGGATTTTAGGAAATTTCTGTTATTAAAATGAATAAATATTTGAAATTAACAAGTATTATCTTTTGAAATAAAAATCTGAGAATGCCAAAAAGTACGTTAGATTCCAAAAAAATGTGATTGGTTTAATTTGTTTTTGTAAAATCATCTTTGAGTGGAAAAATTAAAAGCAAATTGATGTACGGAATCATTAGGTGGGAGATGGGAACACGGACCATTACTGCTGGCAGCGGCCGGAGGACATGACGACGGACCGCCACGCTTACAAGATCGACCCAAGCAATCCCGGGTCGGATCTCGCCGGAGAAACCGCCGCCGCTATGGCCGCCGCCTCCATCGTCTTCCGCCACTACAACCCTTCCTATGCCAATGAGCTCCTTTCCCATGCTCGCCAGGTTAGCCATTTTTATCATCTTTTTTATTTTTATTTCCACCAATTATGTATTCTTTTTTATTTTTCTGAAATGTATAAAATCATCCACAATAAAAGAAAATGAAAATAATTTTACAATTTAAAGCGTAGGCTAGCGGTACCCCACAGCACATGATACTATTAACTACCGCAGTTAATAGGTTTATTTATTGTGATATTGAAAGCTGTTGTTTTTTTAATAAGAAACATTTATTGTGTAATAAAAAAATATATTTTTTTTCTCAATTGAAAAAAATATTTTAAAGAGAATGTTTAAGAAATGTTTGGGTAAATTATAAACTTAGTTTTAGTTGGAGATGAGTTATAATTATAGTTTAGTCTGTAGAGTTAATTTAACAAAAATATTTATAGTGAATAAAAATAGGATTTTTAATATTTTGTTGGAAAAAAATAGCATAAAAATAGGCATGTGATAAAACTGTCGGTGGGAACTGTTTGGGGATGTGCATGTAGATGTGAAGTTACGGGAATGCCATTGGAGAGATTTAATAAATTTGTTTATTAAAAATGTATTTTGTTTGTCAGCTGTTTGAATTTGCGGATAAATACAGAGGAAAGTACGACAGCAGCATCACTGTGGCTCAGAAGTATTATCGATCCGTTAGTGGATACAATGTGCGTAAGCGTATCATCCTTCCCCTTTTCTTGGGCCCTTCTTCTATGGAAGCCCGTTGCTTACTGATTATTACTCACCTATCCCTTTGTTATGGGTTATTTGCAACGTTACTCCTGGAAATGATGACAATAATTCTGTAATTATAATTTGCAGGATGAGTTATTGTGGGCAGCAGCGTGGCTGTATGAGGCAACAAACAACCAATATTACTTGAATTACTTGGGAGAAAATGGTGACTCCATGGGAGGAACTGGTTGGAGTATGACTGAATTTGGTTGGGATGTCAAATATGCTGGTGTCCAAACCCTTGTTGCCAAGGTTGGTTTCCATTTTATTTTTTACATTTCTTTTTATCAAAAAAAAAAAATTATATACATTTAGAACTTTATGGGTGATTTAGACCCGACTATAACTGGGTGGAGTGACCACTACTTATTTGGAGTTTAAACAATAATAGTTGGTGTGACCACTGCAATTTTCTATTTCAAACTCTCTTTATTACAATATTTATCGTTTCTTGTTTGTTACATTGTAAAGTCATTTAAGTACAAATTATTATAATTTATATACTATAATAACTGATTTAATTCTCTTAAACCCTTGTTATAGTTCAAAAGTTGGTAAGGAAGTGTTATATACTATAATAACTGATTTAATGCTCTTAAACCCTTGTTATAGTTCAAAAGTTGGTAAGGAAGTGCTCTGAAAAAGACACCTAATAAGTTCATAACTTTTCAATTCAACTTTGTGTTAAAAGAATCAAAAGTTTAATGAGCTATTGGCACACAAATTTAATTTATAGTAAATAAGTAAGTTTTGTTTGAGAGGGTTTGGTTCTTGCAATTTGTGGATTGTAGATTTATTAATTCTTAAAAAGTTGAATGTTGAGTTTGAAGTAATTTTGATGTTTGTGATCGCAGTTTTTATTGCAAGGAAGAGCGGCTCACCATGCGCCGGTGTTCGAGAGGTACAAGGAGAAGGCTGAAAATTTCTTATGTTCTTGCATGAAGAAAGGCACTCGCAATGTTCAGACCACACCGGCCGGTCTCATCTTCCGTCAGAGATGGAACAATATGCAGTTTGTTACCAGCGCTTCTTTCCTCGCCACCGTCTACTCAGACTACCTTTCTTCCTCTGGTGGAAACTTGAAGTGCGCCGCCGGTTATGTCCAGCCTTCCGAGCTTCTTCGCTTTGCACAATCCCAGGTCCCTACATTTTCTCTTCAAATGGGAACAACTAGTTTTTGATCTTCAAGGTCAATGTTTGTTAACTGTTCGACGAATTGCCTAAATGGGGTTTTGATTTTGAACCAGGTGGATTACATTCTTGGGGACAATCCAAGAGCTACTAGTTACATGGTGGGATATGGAAACAATTACCCACGACAAGTCCATCACAGGGGATCTTCCATTGTTTCAATCAAGGTCAATTCCGCCTTTGTGAGCTGCAGGGGTGGCTATGCAACATGGTACACTAGAAAGGCAAGTGACCCAAACCTACTCACTGGTGCTCTTGTGGGTGGACCTGATGCCTATGACAACTTTGCTGATCAAAGGGACAATTATGAGCAGACTGAGCCTGCTACTTATAACAATGCCCCAATTCTTGGCATTTTAGCCAGGCTCAGTGGTGGACATAGTGGCTACAATCAGCTCCTTCCAGGTACTTACCTCATTTTGATCATCAGATTTCTCTTAAAGCATTTTCAAGTGCAATCATGGCATTGAGCATCCTTTTGTTTTCTCTCCAGTGCTTCTTCCTCCAGTTAATCATCCAGACCCATCTGCACAATCCAAGACACTTCCATCTCCAGGTATGATGCCTTTGCAATTATTTGGTTGGCTTAGAATACTTTGAACAGATCATTTTCTATATCACATGAACTGTTTGGTACTTAAGATGTCCATCACAAAAGGTTCTTCATGCCAACCAATCCAATTGGATTTTTAAGAAATAGTTGATGAATGACTAAATTGTGGCATAAAGCTGGTTTAATTCAAGAAATAGAAACTATTAGGATGAATGACTAAATTGGTCATGGAAGGCGAAAATTTGACAGAAAAGCTTGTTTTTGCAGCATCGTCTTCTACCCCAATTACCGTAGAGCAAAAGATGACGACTTCCTGGACTGCTAAAGGAAATACCTACTATAGATACTCTACAAAAGTGACCAACAAGTCACCAAAAACTCTCAAAAACCTGAAACTTTCGATATCTCAACTTTATGGGCCTCTCTGGGGGCTGGAGAAATCTGGTGAATCATATGTCTTCCCATCTTGGCTTAACTCATTAGCTGCAGGAGAAAGCCTTGAATTTGTTTACATTCACACTGCTTCTCAAGCCAAAATCTCGGTTTCCAGCTACACTCTCTCCTAAATATGAGGGAAGAATCTTGGAAACCAAATCAAGGCAATACAGCATACAAGCTTATGCGGTTCATGGAGTGGTGTAAGAATTGTGCAGTTATAGATAGCGAGAAAGTCCATATTTACGCGTGATATGTATTTGCTTAGGTTTTTTTTAGATGCAATTAGGCCAAATAATGGTGCTGAAGTTTGGGGGAAGAAAGGAGAGAAGAGAGTTTGAAACGGTGCTCATCCTCTTTCTCCACCCTCTTCAATGCTTTATAGACAGAATGAACAGCTGAGAGTGTCAGAGAAGGCAAACAAAGATGGCTTCTCAAAGGATTTGCAAGAGAGTTCTCATTTCTTTCCTTGTATTTTGAATTAGCTTTATGTATCCTTTTAGATGTGTGATTTGCAACATTTTCTTGATTCACACTCGAATGTAGTTTGTGAAACATGGTGTGCTTTTGCTTATCTTGTTTTTAAGATATTGTAAATTCATCACTATACTCAAAACCTTTTGTGTTACTTTTATGGTAAAGTGATTTTAATGGTTTGAAATGTTCTTAAACCATTGGGACATGGCATTTGTACCTTGAAAACAATGAATGTCAATCAATACAACTACTTTTGAAGTTTTTTCATTC

Coding sequence (CDS)

CCTCCAAGCTTATATAGTGCTCCCATCCTCACTTCCACTCCCCACCCCACACTCTCCTTCTTGTTCATTCTTCATTTCTCTCTTCCCCTCTTTAGGGGTTCTTCTCCTCTGTTTCATAATCGATTTATCACAACTTTTATCTTGATAAATCCCACCGGCATTGCCATTTCCTTCTCCCATTCCTTCAAAATGGCAAATCCCATTTCCCCCATTGCTCTCTTTATCCTACTTCTAGCCCCCTCTGCCCTAGCCGGCCATGATTACGGCCAAGCTCTCACCAAGAGCCTTCTCTTCTTCGAAGCTCAGAGATCCGGCTACCTCCCCCACAACCAGCGCGTCAATTGGCGAGCCAATTCCGGCCTCCAAGACGGCAAGGCTAGCGGGGTGGATCTGGTCGGAGGATACTACGACGCCGGTGACAATGTGAAGTTCGGACTTCCGATGGCGTTCACGGTGACGATGATGAGTTGGAGTATTATTGAATATGGAAAACAAATGGCCACTAATGGCGAACTTAGCCATGCCCTAGACGCCGTCAAATGGGGCACCGATTACTTCATCAAAGCCCACCCTGAACCCTATGTTCTCTATGGAGAGGTGGGAGATGGGAACACGGACCATTACTGCTGGCAGCGGCCGGAGGACATGACGACGGACCGCCACGCTTACAAGATCGACCCAAGCAATCCCGGGTCGGATCTCGCCGGAGAAACCGCCGCCGCTATGGCCGCCGCCTCCATCGTCTTCCGCCACTACAACCCTTCCTATGCCAATGAGCTCCTTTCCCATGCTCGCCAGGTTAGCCATTTTTATCATCTTTTTTATTTTTATTTCCACCAATTATGTATTCTTTTTTATTTTTCTGAAATGTATAAAATCATCCACAATAAAAGAAAATGA

Protein sequence

PPSLYSAPILTSTPHPTLSFLFILHFSLPLFRGSSPLFHNRFITTFILINPTGIAISFSHSFKMANPISPIALFILLLAPSALAGHDYGQALTKSLLFFEAQRSGYLPHNQRVNWRANSGLQDGKASGVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMATNGELSHALDAVKWGTDYFIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKIDPSNPGSDLAGETAAAMAAASIVFRHYNPSYANELLSHARQVSHFYHLFYFYFHQLCILFYFSEMYKIIHNKRK

Homology

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BLAST of Cucsat.G11973.T10 vs. ExPASy Swiss-Prot
Match: Q42059 (Endoglucanase 6 OS=Arabidopsis thaliana OX=3702 GN=At1g64390 PE=2 SV=2)

HSP 1 Score: 334.0 bits (855), Expect = 1.8e-90
Identity = 158/204 (77.45%), Postives = 179/204 (87.75%), Query Frame = 0


Query: 69  SPIALFILLLA--PSALAGHDYGQALTKSLLFFEAQRSGYLPHNQRVNWRANSGLQDGKA 128
           +P+A  +LLL   P A +GHDYGQAL+KSLLFFEAQRSG LP NQRV WR++SGL DGK+
Sbjct: 5   APVAALLLLLLCFPVAFSGHDYGQALSKSLLFFEAQRSGVLPRNQRVTWRSHSGLTDGKS 64

Query: 129 SGVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMATNGELSHALDAVKWGTDYF 188
           SGV+LVGGYYDAGDNVKFGLPMAFTVTMM+WS+IEYG Q+  NGEL +++DA+KWGTDYF
Sbjct: 65  SGVNLVGGYYDAGDNVKFGLPMAFTVTMMAWSVIEYGNQLQANGELGNSIDAIKWGTDYF 124

Query: 189 IKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKIDPSNPGSDLAGETAAAMAAAS 248
           IKAHPEP VLYGEVGDGNTDHYCWQRPE+MTTDR AY+IDPSNPGSDLAGETAAAMAAAS
Sbjct: 125 IKAHPEPNVLYGEVGDGNTDHYCWQRPEEMTTDRKAYRIDPSNPGSDLAGETAAAMAAAS 184

Query: 249 IVFRHYNPSYANELLSHARQVSHF 271
           IVFR  NP Y+  LL+HA Q+  F
Sbjct: 185 IVFRRSNPVYSRLLLTHAYQLFDF 208

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BLAST of Cucsat.G11973.T10 vs. ExPASy Swiss-Prot
Match: Q8L7I0 (Endoglucanase 19 OS=Arabidopsis thaliana OX=3702 GN=At4g11050 PE=2 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 1.3e-88
Identity = 156/203 (76.85%), Postives = 177/203 (87.19%), Query Frame = 0


Query: 68  ISPIALFILLLAPSALAGHDYGQALTKSLLFFEAQRSGYLPHNQRVNWRANSGLQDGKAS 127
           IS + + +L L   A++GHDY QAL+KS+LFFEAQRSG+LP NQRV+WR++SGL DGK+S
Sbjct: 7   ISILVVLLLGLVQLAISGHDYKQALSKSILFFEAQRSGHLPPNQRVSWRSHSGLYDGKSS 66

Query: 128 GVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMATNGELSHALDAVKWGTDYFI 187
           GVDLVGGYYDAGDNVKFGLPMAFTVT M WSIIEYG Q+ +NGEL HA+DAVKWGTDYFI
Sbjct: 67  GVDLVGGYYDAGDNVKFGLPMAFTVTTMCWSIIEYGGQLESNGELGHAIDAVKWGTDYFI 126

Query: 188 KAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKIDPSNPGSDLAGETAAAMAAASI 247
           KAHPEP VLYGEVGDG +DHYCWQRPE+MTTDR AYKID +NPGSDLAGETAAAMAAASI
Sbjct: 127 KAHPEPNVLYGEVGDGKSDHYCWQRPEEMTTDRRAYKIDRNNPGSDLAGETAAAMAAASI 186

Query: 248 VFRHYNPSYANELLSHARQVSHF 271
           VFR  +PSY+ ELL HA Q+  F
Sbjct: 187 VFRRSDPSYSAELLRHAHQLFEF 209

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BLAST of Cucsat.G11973.T10 vs. ExPASy Swiss-Prot
Match: Q5NAT0 (Endoglucanase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU5 PE=1 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 1.1e-84
Identity = 148/206 (71.84%), Postives = 173/206 (83.98%), Query Frame = 0


Query: 71  IALFILLLAPSAL------AGHDYGQALTKSLLFFEAQRSGYLPHNQRVNWRANSGLQDG 130
           IAL +L+ A  A        GHDYG AL+KS+L+FEAQRSG LP +QR+ WRANSGL DG
Sbjct: 18  IALVVLVFAAMAQVARGGGGGHDYGMALSKSILYFEAQRSGVLPGSQRIAWRANSGLADG 77

Query: 131 KASGVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMATNGELSHALDAVKWGTD 190
           KA+GVDLVGGYYDAGDNVKFGLPMAFTVTMM+WS+IEYG++MA  GEL HA++A+KWGTD
Sbjct: 78  KANGVDLVGGYYDAGDNVKFGLPMAFTVTMMAWSVIEYGEEMAAAGELGHAVEAIKWGTD 137

Query: 191 YFIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKIDPSNPGSDLAGETAAAMAA 250
           YF KAHPEP VLY EVGDG++DH CWQRPEDMTT R AY++DP NPGSDLAGETAAAMAA
Sbjct: 138 YFAKAHPEPNVLYAEVGDGDSDHNCWQRPEDMTTSRQAYRLDPQNPGSDLAGETAAAMAA 197

Query: 251 ASIVFRHYNPSYANELLSHARQVSHF 271
           AS+VFR  NP YA++LL H++Q+  F
Sbjct: 198 ASLVFRSSNPGYADQLLQHSKQLFDF 223

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BLAST of Cucsat.G11973.T10 vs. ExPASy Swiss-Prot
Match: Q6L4I2 (Endoglucanase 15 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0212300 PE=2 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 2.8e-83
Identity = 146/186 (78.49%), Postives = 163/186 (87.63%), Query Frame = 0


Query: 85  GHDYGQALTKSLLFFEAQRSGYLPHNQRVNWRANSGLQDGKASGVDLVGGYYDAGDNVKF 144
           GHDY  AL KS+L+F+AQRSG LP NQRV+WRA+SGL DGKA+GVDLVGGYYDAGDNVKF
Sbjct: 32  GHDYAMALRKSILYFQAQRSGVLPPNQRVSWRASSGLFDGKANGVDLVGGYYDAGDNVKF 91

Query: 145 GLPMAFTVTMMSWSIIEYGKQMATNGELSHALDAVKWGTDYFIKAHPEPYVLYGEVGDGN 204
           GLPMAFTVTMMSWSI+EYGKQMA  GEL +A+DAVKWGTDYFIKAHPEP VLYGEVGDG+
Sbjct: 92  GLPMAFTVTMMSWSILEYGKQMAAAGELRNAMDAVKWGTDYFIKAHPEPDVLYGEVGDGD 151

Query: 205 TDHYCWQRPEDMTTDRHAYKIDPSNPGSDLAGETAAAMAAASIVFRHYNPSYANELLSHA 264
           TDH CWQRPEDMTT R A+++DP +PGSDLA ETAAAMAAASIVFR   P YAN LL H+
Sbjct: 152 TDHSCWQRPEDMTTSRQAFRVDPQHPGSDLAAETAAAMAAASIVFRGTYPGYANLLLVHS 211

Query: 265 RQVSHF 271
           +Q+  F
Sbjct: 212 KQLFEF 217

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BLAST of Cucsat.G11973.T10 vs. ExPASy Swiss-Prot
Match: Q5NAT8 (Endoglucanase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU7 PE=2 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 3.6e-83
Identity = 147/216 (68.06%), Postives = 174/216 (80.56%), Query Frame = 0


Query: 61  SFKMANPISPIALFILLLAPSALAG------HDYGQALTKSLLFFEAQRSGYLPHNQRVN 120
           S  MAN +  +AL +   A ++  G      HDY  AL+KS+L+FEAQRSG LP NQR+ 
Sbjct: 10  SSSMANLLG-VALVLAATAQTSARGGGGGGRHDYRMALSKSILYFEAQRSGVLPGNQRIA 69

Query: 121 WRANSGLQDGKASGVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMATNGELSH 180
           WRANSGL DGKA+GVDLVGGYYDAGDNVKFG PMAFTVTMM+WS++EYGKQMA  GEL H
Sbjct: 70  WRANSGLADGKANGVDLVGGYYDAGDNVKFGFPMAFTVTMMAWSVLEYGKQMAAAGELGH 129

Query: 181 ALDAVKWGTDYFIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKIDPSNPGSDL 240
           A+DAV+WG DYF+KAHP P VLYGEVGDG++DH CWQRPEDMTT R AY++DP +PGSDL
Sbjct: 130 AMDAVRWGADYFVKAHPAPNVLYGEVGDGDSDHVCWQRPEDMTTSRQAYRLDPQHPGSDL 189

Query: 241 AGETAAAMAAASIVFRHYNPSYANELLSHARQVSHF 271
           AGETA A+AAAS+VFR  NP YAN+LL H++Q+  F
Sbjct: 190 AGETATALAAASLVFRSSNPGYANQLLQHSKQLFDF 224

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BLAST of Cucsat.G11973.T10 vs. NCBI nr
Match: XP_004149748.1 (endoglucanase 6 [Cucumis sativus] >KGN53401.1 hypothetical protein Csa_014642 [Cucumis sativus])

HSP 1 Score: 424 bits (1089), Expect = 4.77e-142
Identity = 204/207 (98.55%), Postives = 205/207 (99.03%), Query Frame = 0


Query: 64  MANPISPIALFILLLAPSALAGHDYGQALTKSLLFFEAQRSGYLPHNQRVNWRANSGLQD 123
           MANPISPIALFILLLAPSALAGHDYGQALTKSLLFFEAQRSGYLPHNQRVNWRANSGLQD
Sbjct: 1   MANPISPIALFILLLAPSALAGHDYGQALTKSLLFFEAQRSGYLPHNQRVNWRANSGLQD 60

Query: 124 GKASGVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMATNGELSHALDAVKWGT 183
           GKASGVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMATNGELSHALDAVKWGT
Sbjct: 61  GKASGVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMATNGELSHALDAVKWGT 120

Query: 184 DYFIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKIDPSNPGSDLAGETAAAMA 243
           DYFIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKIDPSNPGSDLAGETAAAMA
Sbjct: 121 DYFIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKIDPSNPGSDLAGETAAAMA 180

Query: 244 AASIVFRHYNPSYANELLSHARQVSHF 270
           AASIVFRHYNPSYANELLSHARQ+  F
Sbjct: 181 AASIVFRHYNPSYANELLSHARQLFEF 207

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BLAST of Cucsat.G11973.T10 vs. NCBI nr
Match: KAA0044906.1 (endoglucanase 6-like [Cucumis melo var. makuwa])

HSP 1 Score: 417 bits (1071), Expect = 2.49e-139
Identity = 199/207 (96.14%), Postives = 202/207 (97.58%), Query Frame = 0


Query: 64  MANPISPIALFILLLAPSALAGHDYGQALTKSLLFFEAQRSGYLPHNQRVNWRANSGLQD 123
           MANPISPIALF LLLAPS LAGHDYGQALTKS+LFFEAQRSGYLPHNQRVNWRANSGLQD
Sbjct: 1   MANPISPIALFFLLLAPSVLAGHDYGQALTKSILFFEAQRSGYLPHNQRVNWRANSGLQD 60

Query: 124 GKASGVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMATNGELSHALDAVKWGT 183
           GKASGVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMA+NGEL HALDAVKWGT
Sbjct: 61  GKASGVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMASNGELGHALDAVKWGT 120

Query: 184 DYFIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKIDPSNPGSDLAGETAAAMA 243
           DYFIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKIDPSNPGSDLAGETAAAMA
Sbjct: 121 DYFIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKIDPSNPGSDLAGETAAAMA 180

Query: 244 AASIVFRHYNPSYANELLSHARQVSHF 270
           AASIVFRHYNPSYANELLSHARQ+  F
Sbjct: 181 AASIVFRHYNPSYANELLSHARQLFEF 207

[Close]

BLAST of Cucsat.G11973.T10 vs. NCBI nr
Match: XP_008451938.1 (PREDICTED: endoglucanase 6-like [Cucumis melo] >TYK16564.1 endoglucanase 6-like [Cucumis melo var. makuwa])

HSP 1 Score: 417 bits (1071), Expect = 2.49e-139
Identity = 199/207 (96.14%), Postives = 202/207 (97.58%), Query Frame = 0


Query: 64  MANPISPIALFILLLAPSALAGHDYGQALTKSLLFFEAQRSGYLPHNQRVNWRANSGLQD 123
           MANPISPIALF LLLAPS LAGHDYGQALTKS+LFFEAQRSGYLPHNQRVNWRANSGLQD
Sbjct: 1   MANPISPIALFFLLLAPSVLAGHDYGQALTKSILFFEAQRSGYLPHNQRVNWRANSGLQD 60

Query: 124 GKASGVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMATNGELSHALDAVKWGT 183
           GKASGVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMA+NGEL HALDAVKWGT
Sbjct: 61  GKASGVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMASNGELGHALDAVKWGT 120

Query: 184 DYFIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKIDPSNPGSDLAGETAAAMA 243
           DYFIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKIDPSNPGSDLAGETAAAMA
Sbjct: 121 DYFIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKIDPSNPGSDLAGETAAAMA 180

Query: 244 AASIVFRHYNPSYANELLSHARQVSHF 270
           AASIVFRHYNPSYANELLSHARQ+  F
Sbjct: 181 AASIVFRHYNPSYANELLSHARQLFEF 207

[Close]

BLAST of Cucsat.G11973.T10 vs. NCBI nr
Match: XP_023522541.1 (endoglucanase 6-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 394 bits (1011), Expect = 3.44e-135
Identity = 186/207 (89.86%), Postives = 194/207 (93.72%), Query Frame = 0


Query: 64  MANPISPIALFILLLAPSALAGHDYGQALTKSLLFFEAQRSGYLPHNQRVNWRANSGLQD 123
           M  PI PIALF+++ APSAL GHDYGQAL+KS+LFFEAQRSGYLPHNQRV WRANSGLQD
Sbjct: 1   MEIPILPIALFLVICAPSALGGHDYGQALSKSILFFEAQRSGYLPHNQRVTWRANSGLQD 60

Query: 124 GKASGVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMATNGELSHALDAVKWGT 183
           GKASGVDL GGYYDAGDNVKFGLPMAFTVTMMSWSI+EYGKQMA NGEL HALDAVKWGT
Sbjct: 61  GKASGVDLTGGYYDAGDNVKFGLPMAFTVTMMSWSIVEYGKQMAANGELGHALDAVKWGT 120

Query: 184 DYFIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKIDPSNPGSDLAGETAAAMA 243
           DY IKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDR AYKIDPSNPGSDLAGETAAAMA
Sbjct: 121 DYLIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRRAYKIDPSNPGSDLAGETAAAMA 180

Query: 244 AASIVFRHYNPSYANELLSHARQVSHF 270
           AAS+VFRHYNPSYANELLSHARQ+  F
Sbjct: 181 AASLVFRHYNPSYANELLSHARQLFEF 207

[Close]

BLAST of Cucsat.G11973.T10 vs. NCBI nr
Match: XP_038893552.1 (endoglucanase 6 [Benincasa hispida])

HSP 1 Score: 405 bits (1042), Expect = 5.90e-135
Identity = 193/207 (93.24%), Postives = 199/207 (96.14%), Query Frame = 0


Query: 64  MANPISPIALFILLLAPSALAGHDYGQALTKSLLFFEAQRSGYLPHNQRVNWRANSGLQD 123
           M NPIS IALF+LLLAPS LAGHDYGQALTKS+LFFEAQRSGYLPHNQRVNWRANSGLQD
Sbjct: 1   MENPISAIALFLLLLAPSVLAGHDYGQALTKSILFFEAQRSGYLPHNQRVNWRANSGLQD 60

Query: 124 GKASGVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMATNGELSHALDAVKWGT 183
           GKASGVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMA+NGEL HALDAVKWGT
Sbjct: 61  GKASGVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMASNGELGHALDAVKWGT 120

Query: 184 DYFIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKIDPSNPGSDLAGETAAAMA 243
           DY IKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDR AYKIDPSNPGSDLAGETAAAMA
Sbjct: 121 DYLIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRRAYKIDPSNPGSDLAGETAAAMA 180

Query: 244 AASIVFRHYNPSYANELLSHARQVSHF 270
           AAS+VFRHYNP+YANELLSHARQ+  F
Sbjct: 181 AASVVFRHYNPAYANELLSHARQLFEF 207

[Close]

BLAST of Cucsat.G11973.T10 vs. ExPASy TrEMBL
Match: A0A0A0KZU5 (Endoglucanase OS=Cucumis sativus OX=3659 GN=Csa_4G051530 PE=3 SV=1)

HSP 1 Score: 424 bits (1089), Expect = 2.31e-142
Identity = 204/207 (98.55%), Postives = 205/207 (99.03%), Query Frame = 0


Query: 64  MANPISPIALFILLLAPSALAGHDYGQALTKSLLFFEAQRSGYLPHNQRVNWRANSGLQD 123
           MANPISPIALFILLLAPSALAGHDYGQALTKSLLFFEAQRSGYLPHNQRVNWRANSGLQD
Sbjct: 1   MANPISPIALFILLLAPSALAGHDYGQALTKSLLFFEAQRSGYLPHNQRVNWRANSGLQD 60

Query: 124 GKASGVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMATNGELSHALDAVKWGT 183
           GKASGVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMATNGELSHALDAVKWGT
Sbjct: 61  GKASGVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMATNGELSHALDAVKWGT 120

Query: 184 DYFIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKIDPSNPGSDLAGETAAAMA 243
           DYFIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKIDPSNPGSDLAGETAAAMA
Sbjct: 121 DYFIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKIDPSNPGSDLAGETAAAMA 180

Query: 244 AASIVFRHYNPSYANELLSHARQVSHF 270
           AASIVFRHYNPSYANELLSHARQ+  F
Sbjct: 181 AASIVFRHYNPSYANELLSHARQLFEF 207

[Close]

BLAST of Cucsat.G11973.T10 vs. ExPASy TrEMBL
Match: A0A5A7TRY4 (Endoglucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G002000 PE=3 SV=1)

HSP 1 Score: 417 bits (1071), Expect = 1.20e-139
Identity = 199/207 (96.14%), Postives = 202/207 (97.58%), Query Frame = 0


Query: 64  MANPISPIALFILLLAPSALAGHDYGQALTKSLLFFEAQRSGYLPHNQRVNWRANSGLQD 123
           MANPISPIALF LLLAPS LAGHDYGQALTKS+LFFEAQRSGYLPHNQRVNWRANSGLQD
Sbjct: 1   MANPISPIALFFLLLAPSVLAGHDYGQALTKSILFFEAQRSGYLPHNQRVNWRANSGLQD 60

Query: 124 GKASGVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMATNGELSHALDAVKWGT 183
           GKASGVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMA+NGEL HALDAVKWGT
Sbjct: 61  GKASGVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMASNGELGHALDAVKWGT 120

Query: 184 DYFIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKIDPSNPGSDLAGETAAAMA 243
           DYFIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKIDPSNPGSDLAGETAAAMA
Sbjct: 121 DYFIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKIDPSNPGSDLAGETAAAMA 180

Query: 244 AASIVFRHYNPSYANELLSHARQVSHF 270
           AASIVFRHYNPSYANELLSHARQ+  F
Sbjct: 181 AASIVFRHYNPSYANELLSHARQLFEF 207

[Close]

BLAST of Cucsat.G11973.T10 vs. ExPASy TrEMBL
Match: A0A5D3D018 (Endoglucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G003840 PE=3 SV=1)

HSP 1 Score: 417 bits (1071), Expect = 1.20e-139
Identity = 199/207 (96.14%), Postives = 202/207 (97.58%), Query Frame = 0


Query: 64  MANPISPIALFILLLAPSALAGHDYGQALTKSLLFFEAQRSGYLPHNQRVNWRANSGLQD 123
           MANPISPIALF LLLAPS LAGHDYGQALTKS+LFFEAQRSGYLPHNQRVNWRANSGLQD
Sbjct: 1   MANPISPIALFFLLLAPSVLAGHDYGQALTKSILFFEAQRSGYLPHNQRVNWRANSGLQD 60

Query: 124 GKASGVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMATNGELSHALDAVKWGT 183
           GKASGVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMA+NGEL HALDAVKWGT
Sbjct: 61  GKASGVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMASNGELGHALDAVKWGT 120

Query: 184 DYFIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKIDPSNPGSDLAGETAAAMA 243
           DYFIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKIDPSNPGSDLAGETAAAMA
Sbjct: 121 DYFIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKIDPSNPGSDLAGETAAAMA 180

Query: 244 AASIVFRHYNPSYANELLSHARQVSHF 270
           AASIVFRHYNPSYANELLSHARQ+  F
Sbjct: 181 AASIVFRHYNPSYANELLSHARQLFEF 207

[Close]

BLAST of Cucsat.G11973.T10 vs. ExPASy TrEMBL
Match: A0A1S3BS30 (Endoglucanase OS=Cucumis melo OX=3656 GN=LOC103493091 PE=3 SV=1)

HSP 1 Score: 417 bits (1071), Expect = 1.20e-139
Identity = 199/207 (96.14%), Postives = 202/207 (97.58%), Query Frame = 0


Query: 64  MANPISPIALFILLLAPSALAGHDYGQALTKSLLFFEAQRSGYLPHNQRVNWRANSGLQD 123
           MANPISPIALF LLLAPS LAGHDYGQALTKS+LFFEAQRSGYLPHNQRVNWRANSGLQD
Sbjct: 1   MANPISPIALFFLLLAPSVLAGHDYGQALTKSILFFEAQRSGYLPHNQRVNWRANSGLQD 60

Query: 124 GKASGVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMATNGELSHALDAVKWGT 183
           GKASGVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMA+NGEL HALDAVKWGT
Sbjct: 61  GKASGVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMASNGELGHALDAVKWGT 120

Query: 184 DYFIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKIDPSNPGSDLAGETAAAMA 243
           DYFIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKIDPSNPGSDLAGETAAAMA
Sbjct: 121 DYFIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKIDPSNPGSDLAGETAAAMA 180

Query: 244 AASIVFRHYNPSYANELLSHARQVSHF 270
           AASIVFRHYNPSYANELLSHARQ+  F
Sbjct: 181 AASIVFRHYNPSYANELLSHARQLFEF 207

[Close]

BLAST of Cucsat.G11973.T10 vs. ExPASy TrEMBL
Match: A0A6J1IX13 (Endoglucanase OS=Cucurbita maxima OX=3661 GN=LOC111479337 PE=3 SV=1)

HSP 1 Score: 397 bits (1021), Expect = 4.19e-132
Identity = 187/207 (90.34%), Postives = 195/207 (94.20%), Query Frame = 0


Query: 64  MANPISPIALFILLLAPSALAGHDYGQALTKSLLFFEAQRSGYLPHNQRVNWRANSGLQD 123
           M NPI PIALF+++ APSAL GHDYGQAL+KS+LFFEAQRSGYLPHNQRV WRANSGLQD
Sbjct: 1   MENPILPIALFLVIFAPSALGGHDYGQALSKSILFFEAQRSGYLPHNQRVTWRANSGLQD 60

Query: 124 GKASGVDLVGGYYDAGDNVKFGLPMAFTVTMMSWSIIEYGKQMATNGELSHALDAVKWGT 183
           GKASGVDL GGYYDAGDNVKFGLPMAFTVTMMSWSI+EYGKQMA NGEL HALDAVKWGT
Sbjct: 61  GKASGVDLTGGYYDAGDNVKFGLPMAFTVTMMSWSIVEYGKQMAANGELGHALDAVKWGT 120

Query: 184 DYFIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKIDPSNPGSDLAGETAAAMA 243
           DY IKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDR AYKIDPSNPGSDLAGETAAAMA
Sbjct: 121 DYLIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRRAYKIDPSNPGSDLAGETAAAMA 180

Query: 244 AASIVFRHYNPSYANELLSHARQVSHF 270
           AAS+VFRHYNPSYANELLSHARQ+  F
Sbjct: 181 AASLVFRHYNPSYANELLSHARQLFEF 207

The following BLAST results are available for this feature:

BLAST of Cucsat.G11973.T10 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q42059	1.8e-90	77.45	Endoglucanase 6 OS=Arabidopsis thaliana OX=3702 GN=At1g64390 PE=2 SV=2	[more]
Q8L7I0	1.3e-88	76.85	Endoglucanase 19 OS=Arabidopsis thaliana OX=3702 GN=At4g11050 PE=2 SV=1	[more]
Q5NAT0	1.1e-84	71.84	Endoglucanase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU5 PE=1 SV=1	[more]
Q6L4I2	2.8e-83	78.49	Endoglucanase 15 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0212300 PE=2 S...	[more]
Q5NAT8	3.6e-83	68.06	Endoglucanase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU7 PE=2 SV=1	[more]

BLAST of Cucsat.G11973.T10 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004149748.1	4.77e-142	98.55	endoglucanase 6 [Cucumis sativus] >KGN53401.1 hypothetical protein Csa_014642 [C...	[more]
KAA0044906.1	2.49e-139	96.14	endoglucanase 6-like [Cucumis melo var. makuwa]	[more]
XP_008451938.1	2.49e-139	96.14	PREDICTED: endoglucanase 6-like [Cucumis melo] >TYK16564.1 endoglucanase 6-like ...	[more]
XP_023522541.1	3.44e-135	89.86	endoglucanase 6-like, partial [Cucurbita pepo subsp. pepo]	[more]
XP_038893552.1	5.90e-135	93.24	endoglucanase 6 [Benincasa hispida]	[more]

BLAST of Cucsat.G11973.T10 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KZU5	2.31e-142	98.55	Endoglucanase OS=Cucumis sativus OX=3659 GN=Csa_4G051530 PE=3 SV=1	[more]
A0A5A7TRY4	1.20e-139	96.14	Endoglucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G002000 ...	[more]
A0A5D3D018	1.20e-139	96.14	Endoglucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G003840 ...	[more]
A0A1S3BS30	1.20e-139	96.14	Endoglucanase OS=Cucumis melo OX=3656 GN=LOC103493091 PE=3 SV=1	[more]
A0A6J1IX13	4.19e-132	90.34	Endoglucanase OS=Cucurbita maxima OX=3661 GN=LOC111479337 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012341	Six-hairpin glycosidase-like superfamily	GENE3D	1.50.10.10		coord: 83..274 e-value: 4.8E-90 score: 304.8
IPR001701	Glycoside hydrolase family 9	PFAM	PF00759	Glyco_hydro_9	coord: 88..271 e-value: 2.0E-75 score: 254.9
None	No IPR available	PANTHER	PTHR22298:SF133	ENDOGLUCANASE	coord: 74..270
None	No IPR available	PANTHER	PTHR22298	ENDO-1,4-BETA-GLUCANASE	coord: 74..270
IPR008928	Six-hairpin glycosidase superfamily	SUPERFAMILY	48208	Six-hairpin glycosidases	coord: 85..271

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G11973	Cucsat.G11973	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G11973.T10.E1	Cucsat.G11973.T10.E1	exon
Cucsat.G11973.T10.E2	Cucsat.G11973.T10.E2	exon
Cucsat.G11973.T10.E3	Cucsat.G11973.T10.E3	exon
Cucsat.G11973.T10.E4	Cucsat.G11973.T10.E4	exon
Cucsat.G11973.T10.E5	Cucsat.G11973.T10.E5	exon
Cucsat.G11973.T10.E6	Cucsat.G11973.T10.E6	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G11973.T10.C3	Cucsat.G11973.T10.C3	CDS
Cucsat.G11973.T10.C2	Cucsat.G11973.T10.C2	CDS
Cucsat.G11973.T10.C1	Cucsat.G11973.T10.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G11973.T10	Cucsat.G11973.T10-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0030245	cellulose catabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function	GO:0030246	carbohydrate binding
molecular_function	GO:0008810	cellulase activity