Cucsat.G11730.T1 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G11730.T1
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Subtilisin-like protease SBT2.5
Location	ctg1820: 4225922 .. 4227253 (+)
RNA-Seq Expression	Cucsat.G11730.T1
Synteny	Cucsat.G11730.T1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGAAGATGATAATTAGAAACCCTAAACAAAATTTTGACTTTTATTTTCTAACAAAAAAGACGTTATTTTAGTCATTTAAATGTTTTGGGAACTCGACGCCCTTCTCTTCAGTAACCAAAATTGAACTTTATATTTTTAAACAAATATATCTAACCGACCCTAATTAAATTATACTTCCATCATATATATATATGCGTGCGTGTGTGTGTAGTTTATGCATTATTAAAGAGAGTTCAATATTTTCCCTAAGAAGAAGACTACAATGCAGGAGAACAGATACAATGGTGCACATGAACTGGGTGGCAGAGGCGAGGAATGTTACTTCGTTTTCATGAAGTATGATCCTGAATATGAACGTCTTCGGGCTGATCGGTTAGTAAAAGTTGTCACTATAAACATTTTTTTTCTTTTTCTTCTTTTAATATGTTTTCTTTTTTAATTTGATCTTTTTGGTATGAAGATGTGGGGAAGGGGCAGATGAGCTTGATGGGTACCTGAGTAGAAAGCACGATGAGGTTCTGAGGAGGTGGTTGGAGCCTGGCAGTTACAGAAAAATTTCGTCCTTTCTCATCGTTGACGGGTTTTCAGTCGAGATTAACGAACATCAGGTATTTCAAGTTTCGTTAAATAATGAGTTTTAAGAGAACATTCATAGAAGATTGTCAAGACTTCTATGTTCTTAGAAGTATATGCTTTTTGAAAAAAAAAAATTAGAGACTAAAAGAAATGGTAAATTTAAGAAACAATTACTTGGTATTTCTTTCTTCTTTTTCTGGTTTGTTATTAAAAGAAAAAAAAATTAACTTCAGCTTTCTTTTCCCACATAAAGAGTTTAATTTATAAAAAGAAGCCTTTAAAAAATGATTAAAAGTAATAAACTTTTATTTGATATATTCACTTTGCTTTATTTGAAAATGTCCATCCATCTATTTTTTTTTTTTTTAATTTTTGAAGAAAAGAAAATGAGTTTGGTTTTGATGATTTAAATACAGGCAAATGTGCTTAGATATGCAGAGGGAGTAAGGGTGGTGGAGAAGAATCAAGACCAACCTCTCATTATCAATCCAACAACAACTGATTAAAGGTAGCAATTAAAGGAGCAAGGCCAAAAAATAAATTAAATAATAACCATCTTTCTAATTTTCTAAATCAAATCTTTCTATCTCTATTGTCGCTGTACTCTTTTGTTTAGAATATGTTGAATAATTACATTTAATTATTATAGTCTATATATAAAATCTTAATTTGCTACGTGTAACTCAATTGACGTATGTATACATTAACAACTTTAAGGTAAGATGGTCGCAATTCCCCGAGTCCATTTGAACTAAG

Coding sequence (CDS)

ATGCAGGAGAACAGATACAATGGTGCACATGAACTGGGTGGCAGAGGCGAGGAATGTTACTTCGTTTTCATGAAGTATGATCCTGAATATGAACGTCTTCGGGCTGATCGATGTGGGGAAGGGGCAGATGAGCTTGATGGGTACCTGAGTAGAAAGCACGATGAGGTTCTGAGGAGGTGGTTGGAGCCTGGCAGTTACAGAAAAATTTCGTCCTTTCTCATCGTTGACGGGTTTTCAGTCGAGATTAACGAACATCAGGCAAATGTGCTTAGATATGCAGAGGGAGTAAGGGTGGTGGAGAAGAATCAAGACCAACCTCTCATTATCAATCCAACAACAACTGATTAA

Protein sequence

MQENRYNGAHELGGRGEECYFVFMKYDPEYERLRADRCGEGADELDGYLSRKHDEVLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLRYAEGVRVVEKNQDQPLIINPTTTD

Homology

[Close]

BLAST of Cucsat.G11730.T1 vs. ExPASy Swiss-Prot
Match: O64481 (Subtilisin-like protease SBT2.5 OS=Arabidopsis thaliana OX=3702 GN=SBT2.5 PE=2 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 1.2e-05
Identity = 24/56 (42.86%), Postives = 37/56 (66.07%), Query Frame = 0


Query: 48  YLSRKHDEVLRRWLEPGSYRKISSFL-IVDGFSVEINEHQANVLRYAEGVRVVEKN 103
           +L RKHD +L    E GSY+K+ S+  +++GF+  ++  QA  LR A GVR V+K+
Sbjct: 65  HLERKHDMILGMLFEEGSYKKLYSYKHLINGFAAHVSPEQAETLRRAPGVRSVDKD 120

[Close]

BLAST of Cucsat.G11730.T1 vs. ExPASy Swiss-Prot
Match: Q9SZV5 (Subtilisin-like protease SBT2.6 OS=Arabidopsis thaliana OX=3702 GN=SBT2.6 PE=2 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 6.4e-04
Identity = 21/56 (37.50%), Postives = 37/56 (66.07%), Query Frame = 0


Query: 48  YLSRKHDEVLRRWLEPGSYRKISSFL-IVDGFSVEINEHQANVLRYAEGVRVVEKN 103
           +L RKHD +L      GSY+K+ S+  +++GF+  ++  QA +LR A GV+ V+++
Sbjct: 65  HLERKHDMLLGMLFVEGSYKKLYSYKHLINGFAAHVSPDQAEMLRRAPGVKSVDRD 120

[Close]

BLAST of Cucsat.G11730.T1 vs. NCBI nr
Match: XP_011653213.1 (uncharacterized protein LOC105435187 [Cucumis sativus] >KAE8649203.1 hypothetical protein Csa_015401 [Cucumis sativus])

HSP 1 Score: 245 bits (626), Expect = 4.30e-82
Identity = 115/115 (100.00%), Postives = 115/115 (100.00%), Query Frame = 0


Query: 1   MQENRYNGAHELGGRGEECYFVFMKYDPEYERLRADRCGEGADELDGYLSRKHDEVLRRW 60
           MQENRYNGAHELGGRGEECYFVFMKYDPEYERLRADRCGEGADELDGYLSRKHDEVLRRW
Sbjct: 1   MQENRYNGAHELGGRGEECYFVFMKYDPEYERLRADRCGEGADELDGYLSRKHDEVLRRW 60

Query: 61  LEPGSYRKISSFLIVDGFSVEINEHQANVLRYAEGVRVVEKNQDQPLIINPTTTD 115
           LEPGSYRKISSFLIVDGFSVEINEHQANVLRYAEGVRVVEKNQDQPLIINPTTTD
Sbjct: 61  LEPGSYRKISSFLIVDGFSVEINEHQANVLRYAEGVRVVEKNQDQPLIINPTTTD 115

[Close]

BLAST of Cucsat.G11730.T1 vs. NCBI nr
Match: KAA0044911.1 (subtilisin-like protease SBT2.5 [Cucumis melo var. makuwa] >TYK16559.1 subtilisin-like protease SBT2.5 [Cucumis melo var. makuwa])

HSP 1 Score: 230 bits (586), Expect = 6.42e-76
Identity = 110/120 (91.67%), Postives = 113/120 (94.17%), Query Frame = 0


Query: 1   MQENRYNGAHEL-----GGRGEECYFVFMKYDPEYERLRADRCGEGADELDGYLSRKHDE 60
           MQENRYNGAHEL     GGRG ECYFVFMKYDPEYERLRADRCGEGADE+DGYLSRKHDE
Sbjct: 1   MQENRYNGAHELSGGGGGGRGGECYFVFMKYDPEYERLRADRCGEGADEVDGYLSRKHDE 60

Query: 61  VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLRYAEGVRVVEKNQDQPLIINPTTTD 115
           VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLR AEGVRVVEKNQDQPLI+NPT+TD
Sbjct: 61  VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLRSAEGVRVVEKNQDQPLIVNPTSTD 120

[Close]

BLAST of Cucsat.G11730.T1 vs. NCBI nr
Match: XP_008451935.1 (PREDICTED: uncharacterized protein LOC103493088 [Cucumis melo])

HSP 1 Score: 229 bits (583), Expect = 1.84e-75
Identity = 109/120 (90.83%), Postives = 113/120 (94.17%), Query Frame = 0


Query: 1   MQENRYNGAHEL-----GGRGEECYFVFMKYDPEYERLRADRCGEGADELDGYLSRKHDE 60
           MQENRYNGAHEL     GGRG ECYFVFMKYDPEYERLRADRCGEGADE+DGYLSRKHDE
Sbjct: 1   MQENRYNGAHELSGGGGGGRGGECYFVFMKYDPEYERLRADRCGEGADEVDGYLSRKHDE 60

Query: 61  VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLRYAEGVRVVEKNQDQPLIINPTTTD 115
           VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLR A+GVRVVEKNQDQPLI+NPT+TD
Sbjct: 61  VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLRSADGVRVVEKNQDQPLIVNPTSTD 120

[Close]

BLAST of Cucsat.G11730.T1 vs. NCBI nr
Match: XP_038877291.1 (uncharacterized protein LOC120069570 [Benincasa hispida])

HSP 1 Score: 190 bits (482), Expect = 1.08e-59
Identity = 94/113 (83.19%), Postives = 96/113 (84.96%), Query Frame = 0


Query: 1   MQENRYNGAHELGGRGE-----ECYFVFMKYDPEYERLRADRCGEGADELDGYLSRKHDE 60
           MQENRY+G HE GG G      ECYFVFM YDPEYERLRADR GEG DELD YLSRKHDE
Sbjct: 25  MQENRYDGGHEQGGGGGGGCGGECYFVFMNYDPEYERLRADRSGEGVDELDLYLSRKHDE 84

Query: 61  VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLRYAEGVRVVEKNQDQPLI 108
           +L RWLEPGSYRKISSFLIVDGFSVEI E QANVLR AEGVRVVEKNQDQPLI
Sbjct: 85  ILGRWLEPGSYRKISSFLIVDGFSVEITEDQANVLRCAEGVRVVEKNQDQPLI 137

[Close]

BLAST of Cucsat.G11730.T1 vs. NCBI nr
Match: XP_022136905.1 (uncharacterized protein LOC111008483 [Momordica charantia])

HSP 1 Score: 169 bits (428), Expect = 7.44e-52
Identity = 87/110 (79.09%), Postives = 90/110 (81.82%), Query Frame = 0


Query: 1   MQENRYNGAHELGGRGEECYFVFMKYDPEYERLRADRCGEGADELDGYLSRKHDEVLRRW 60
           M ENR  G  + GG   ECYFVFM YDPEYERLRADR G+GA ELD YLSRKHDE+L RW
Sbjct: 1   MPENRNRG--DCGGG--ECYFVFMNYDPEYERLRADRSGKGAHELDLYLSRKHDELLGRW 60

Query: 61  LEPGSYRKISSFLIVDGFSVEINEHQANVLRYAEGVRVVEKNQDQPLIIN 110
           LEP SYRKISSFLIVDGFS EI EHQANVLR AEGVRVVEKNQDQP  IN
Sbjct: 61  LEPTSYRKISSFLIVDGFSAEITEHQANVLRSAEGVRVVEKNQDQPAPIN 106

[Close]

BLAST of Cucsat.G11730.T1 vs. ExPASy TrEMBL
Match: A0A5D3D232 (Subtilisin-like protease SBT2.5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G003780 PE=4 SV=1)

HSP 1 Score: 230 bits (586), Expect = 3.11e-76
Identity = 110/120 (91.67%), Postives = 113/120 (94.17%), Query Frame = 0


Query: 1   MQENRYNGAHEL-----GGRGEECYFVFMKYDPEYERLRADRCGEGADELDGYLSRKHDE 60
           MQENRYNGAHEL     GGRG ECYFVFMKYDPEYERLRADRCGEGADE+DGYLSRKHDE
Sbjct: 1   MQENRYNGAHELSGGGGGGRGGECYFVFMKYDPEYERLRADRCGEGADEVDGYLSRKHDE 60

Query: 61  VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLRYAEGVRVVEKNQDQPLIINPTTTD 115
           VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLR AEGVRVVEKNQDQPLI+NPT+TD
Sbjct: 61  VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLRSAEGVRVVEKNQDQPLIVNPTSTD 120

[Close]

BLAST of Cucsat.G11730.T1 vs. ExPASy TrEMBL
Match: A0A1S3BSM3 (uncharacterized protein LOC103493088 OS=Cucumis melo OX=3656 GN=LOC103493088 PE=4 SV=1)

HSP 1 Score: 229 bits (583), Expect = 8.92e-76
Identity = 109/120 (90.83%), Postives = 113/120 (94.17%), Query Frame = 0


Query: 1   MQENRYNGAHEL-----GGRGEECYFVFMKYDPEYERLRADRCGEGADELDGYLSRKHDE 60
           MQENRYNGAHEL     GGRG ECYFVFMKYDPEYERLRADRCGEGADE+DGYLSRKHDE
Sbjct: 1   MQENRYNGAHELSGGGGGGRGGECYFVFMKYDPEYERLRADRCGEGADEVDGYLSRKHDE 60

Query: 61  VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLRYAEGVRVVEKNQDQPLIINPTTTD 115
           VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLR A+GVRVVEKNQDQPLI+NPT+TD
Sbjct: 61  VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLRSADGVRVVEKNQDQPLIVNPTSTD 120

[Close]

BLAST of Cucsat.G11730.T1 vs. ExPASy TrEMBL
Match: A0A0A0KZT9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G051480 PE=4 SV=1)

HSP 1 Score: 189 bits (479), Expect = 3.02e-60
Identity = 86/86 (100.00%), Postives = 86/86 (100.00%), Query Frame = 0


Query: 1  MQENRYNGAHELGGRGEECYFVFMKYDPEYERLRADRCGEGADELDGYLSRKHDEVLRRW 60
          MQENRYNGAHELGGRGEECYFVFMKYDPEYERLRADRCGEGADELDGYLSRKHDEVLRRW
Sbjct: 1  MQENRYNGAHELGGRGEECYFVFMKYDPEYERLRADRCGEGADELDGYLSRKHDEVLRRW 60

Query: 61 LEPGSYRKISSFLIVDGFSVEINEHQ 86
          LEPGSYRKISSFLIVDGFSVEINEHQ
Sbjct: 61 LEPGSYRKISSFLIVDGFSVEINEHQ 86

[Close]

BLAST of Cucsat.G11730.T1 vs. ExPASy TrEMBL
Match: A0A6J1C587 (uncharacterized protein LOC111008483 OS=Momordica charantia OX=3673 GN=LOC111008483 PE=4 SV=1)

HSP 1 Score: 169 bits (428), Expect = 3.60e-52
Identity = 87/110 (79.09%), Postives = 90/110 (81.82%), Query Frame = 0


Query: 1   MQENRYNGAHELGGRGEECYFVFMKYDPEYERLRADRCGEGADELDGYLSRKHDEVLRRW 60
           M ENR  G  + GG   ECYFVFM YDPEYERLRADR G+GA ELD YLSRKHDE+L RW
Sbjct: 1   MPENRNRG--DCGGG--ECYFVFMNYDPEYERLRADRSGKGAHELDLYLSRKHDELLGRW 60

Query: 61  LEPGSYRKISSFLIVDGFSVEINEHQANVLRYAEGVRVVEKNQDQPLIIN 110
           LEP SYRKISSFLIVDGFS EI EHQANVLR AEGVRVVEKNQDQP  IN
Sbjct: 61  LEPTSYRKISSFLIVDGFSAEITEHQANVLRSAEGVRVVEKNQDQPAPIN 106

[Close]

BLAST of Cucsat.G11730.T1 vs. ExPASy TrEMBL
Match: A0A6J1J855 (subtilisin-like protease SBT2.5 OS=Cucurbita maxima OX=3661 GN=LOC111483437 PE=4 SV=1)

HSP 1 Score: 167 bits (422), Expect = 2.53e-51
Identity = 84/105 (80.00%), Postives = 88/105 (83.81%), Query Frame = 0


Query: 1   MQENRYNGAHELGGRGEECYFVFMKYDPEYERLRADRCGEGADELDGYLSRKHDEVLRRW 60
           MQENRY+G    GG G  CYFVFM YDPEYERLRADR GEGA ELD YLSRKHD VL R 
Sbjct: 1   MQENRYDGVG--GGGGGACYFVFMNYDPEYERLRADRSGEGAHELDEYLSRKHDGVLGRC 60

Query: 61  LEPGSYRKISSFLIVDGFSVEINEHQANVLRYAEGVRVVEKNQDQ 105
           LEPG+YRK+SSFLIVDGFSVEI E QA+VLR AEGVRVVEKNQDQ
Sbjct: 61  LEPGTYRKVSSFLIVDGFSVEITEEQADVLRSAEGVRVVEKNQDQ 103

The following BLAST results are available for this feature:

BLAST of Cucsat.G11730.T1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O64481	1.2e-05	42.86	Subtilisin-like protease SBT2.5 OS=Arabidopsis thaliana OX=3702 GN=SBT2.5 PE=2 S...	[more]
Q9SZV5	6.4e-04	37.50	Subtilisin-like protease SBT2.6 OS=Arabidopsis thaliana OX=3702 GN=SBT2.6 PE=2 S...	[more]

BLAST of Cucsat.G11730.T1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011653213.1	4.30e-82	100.00	uncharacterized protein LOC105435187 [Cucumis sativus] >KAE8649203.1 hypothetica...	[more]
KAA0044911.1	6.42e-76	91.67	subtilisin-like protease SBT2.5 [Cucumis melo var. makuwa] >TYK16559.1 subtilisi...	[more]
XP_008451935.1	1.84e-75	90.83	PREDICTED: uncharacterized protein LOC103493088 [Cucumis melo]	[more]
XP_038877291.1	1.08e-59	83.19	uncharacterized protein LOC120069570 [Benincasa hispida]	[more]
XP_022136905.1	7.44e-52	79.09	uncharacterized protein LOC111008483 [Momordica charantia]	[more]

BLAST of Cucsat.G11730.T1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3D232	3.11e-76	91.67	Subtilisin-like protease SBT2.5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A1S3BSM3	8.92e-76	90.83	uncharacterized protein LOC103493088 OS=Cucumis melo OX=3656 GN=LOC103493088 PE=...	[more]
A0A0A0KZT9	3.02e-60	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G051480 PE=4 SV=1	[more]
A0A6J1C587	3.60e-52	79.09	uncharacterized protein LOC111008483 OS=Momordica charantia OX=3673 GN=LOC111008...	[more]
A0A6J1J855	2.53e-51	80.00	subtilisin-like protease SBT2.5 OS=Cucurbita maxima OX=3661 GN=LOC111483437 PE=4...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	PFAM	PF05922	Inhibitor_I9	coord: 49..103 e-value: 1.7E-5 score: 25.4
None	No IPR available	PANTHER	PTHR37379	OS01G0220500 PROTEIN	coord: 15..104

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G11730	Cucsat.G11730	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G11730.T1.E1	Cucsat.G11730.T1.E1	exon
Cucsat.G11730.T1.E2	Cucsat.G11730.T1.E2	exon
Cucsat.G11730.T1.E3	Cucsat.G11730.T1.E3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G11730.T1.C1	Cucsat.G11730.T1.C1	CDS
Cucsat.G11730.T1.C2	Cucsat.G11730.T1.C2	CDS
Cucsat.G11730.T1.C3	Cucsat.G11730.T1.C3	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G11730.T1	Cucsat.G11730.T1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0008233	peptidase activity