Cucsat.G10300.T1 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G10300.T1
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	NAD(P)H dehydrogenase (quinone)
Location	ctg1675: 849045 .. 852293 (+)
RNA-Seq Expression	Cucsat.G10300.T1
Synteny	Cucsat.G10300.T1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTTGCACTCTGAAAAGAGAAATATTGGAATATATGTGTAGATAAAAGAAAAAATGAGTAAGCAGTGACCACATGTCGTTGTAGGAACCAATAATCCAATAGTAATGAGAATCTAAACTCATCCCCTCAACCTTCATCACAAATCACACTTACTCTGTCTCTCATCATTTTCTCCACCGTTATTATATTCTCTCATTCATCGTCTCTTCATAAACCCTAATCCCAATCCATCCCCATGGCCTCGTTATCCACGGTGGTCGGATCTCAACCGGCTATCAAAGTCATCGCTCTCTGTGGTTCTCTTCGTCAAAACTCTACCAACCGAGGCCTTATTCGTGCCGGTAATTCCTCCATTTACTTTTTACCCTTCTTTTAAAGTTATTGGGTTAAATTGTTCGCTGTTTTTTCCAATATTTTTCATGTGGCTGTTCATTAATATTGCGCAGCAACGGAGATAGCGAAGGAATCCATCGACGGATTGCAAATTGAATATTTGGATATCTCCCCATTGCCGATGCTGAATACCGATTTGGAGGTTGATGGAACATATCCGCCCGCCGTTGAGGCGTTTCGCCGGAAGATTCTTGAAGCTGATAGCATTCTCTTTGCATCGCCGGAATATAACTACTCTGTTACCGGTATATTCCTTTTTCGTTTCTCGTTTTCAATATATAATAATAAGAGATGTCAGCCATTTGTTTTGGAACACGTGTCTTGTCAGCATTAAATGGACCAACGTCACATGTCTCTCTTTAAATATACTTTTGAATTTTCTTTTAAATTTTGGTCTCAAGGTCTCGAGTCACAAAAAAATTATTTAAAATGTTTATGAAAAATAGTAAAATTTTAGAGTGGAAACACTTTCATACATACTCTATAAACTTTCCTCAATAATTATTAATCGTATGTCAATCAAAATATATCAATTCATGTCACACCATTTTTTATATTTATAAAAGTAGCAAATTTAAAAGTGATAGTCCTCTTATAGTGTGATAACAGTTTAATATCATTAATTACTTATCATAGTTGTCATATTCGTAATATGTAAAAAAGATGTGTTATGAACGGTTTTTTTTAAATTTTTTTGTTATTTAATGCAATTTTTTTTAAAATTATTTGGAATGATTGTGACATACTTGATTTTTTCCCAAACCTACCCTTATTTTGATATTTATAACATTTTAGTTTGACTCATTTTGGTTTCTATTTTTTAAAAATGTTTAATTTAGTCTTTTAACTTTTGTTCATTTGAGTTTCCACTCATTTAAGAAAGTGGCTATTTTGGCCTATTCATTTTCATTTCATATTTTTGCTACCACTTTTTAAAAGTCAAAAATACCAAAATGAACCAAAGTTAAATTATAAGAACCACAAACGAACATTTTGAAAATACTTAAATTAAACTTAACCACGACTTAAAGTAAATGGACCAAAATGAATATGTCAAAAATTATAAGGACCAAGACAAACAAAGACCAAAAGTATAATCTTTAAACCTAATAATTCAATCAATTTTACCTCTAAACTTTTGCGCGATTGTATCAAATGGACCAGTTTTCTAATTTTAATTAAGAAACATCATATGGAACGTTATGCCTTGATTGACAACTATTTCGTTTTTTATTTTTGGTGTTTTAAAATTAAGCCGATAAACATCTCATTCACTTCTAAAAATTCTTAGTTTTTTATTTACTTTCTACCCATATTTTTAAACACGGACAAATTTTGAAATTATTTATTTGGTTCTTGAAACAAATACATTCTTAAATTTATAATTTTATGTATTCGTATATGTGGTTGAAAATGAAAAGTGGTATGATATATATCTTTAAATTTTCAATTTTAGGTTAGATATTTTATTTTTCTAAGTTTGTATCTGTCAAATCCTTCACATATCTAACATTTTTTAAAAGAAAATTAACAGATTTATTAAACATCAAATATATATTTTTTCAATCGTTCAATCTTATATATTGTTCTAATTGTGTGTTAGTGTCAGGTGCTTAAGTGAAGACTACTTAACTAGCTTGAATTGTCAACTTACTTATGCTATATTGTTTTGTGTGCATTTAGGAAAAGTTTATTCTTTTACATCAATGAACATTGATATTACACTTTAAATTTTATCAAATTAAATCAACAAAATCCAACCAATATGAGTGACTAAACGATATGTCCTTAAATTTTTTTTTTTAAAAAAAAAAAAACTACTTATTTGATATATATAGTACAGTGTTGGATTCCTCTCTATACCCATATATTGTATTAAAAGAACTTATACATGTTATTTGAGTTTAATTTAGTGGTGGTGGTGATAATAGTGTTATTAGCACAAAGAAATTAAAACCCTTTTATTGGCATAAAGAAAATGACTCGGTTGCCATCCTTCGAGCTTTCTTCTAGATTTTGTTAGGCCAACAAGCGTAATAAGTACCTCTTAATTCATAGACTCCCTTCTCTTTTCTTTTGATTCTTTTATTGATTTGGTTGTGTCTATTCTGCTTTAACGAACTTAATCATTCTTAGTTTTAATTAATTGAAAATCTCTTTTGAATCACCTTTGAACATGCTTGAAGTCTCTCCTATTTCATTTATTTAATGAAATGTTTCTTATCTAAGAGAAACGATGTTGTTATTAGCACCATTGAAGAATGCAATAGACTGGGGATCAAGGCCACCAAATGTATGGGGAGACAAAGCAGCAGCAATAGTTAGTGCAAGTGGTGGCTCTGGTGGTGTAAGATCACAGTACCATCTTCGCCAAATTGGAGTTTTTCTTGACCTTCATTTCATAAACAAGCCTGAGTTTTTCTTGAATGCACATCAGCCACCCCCCAAGTTCAACAGCAGCGGTGACTTGATCGATGCCGAAGTTCGAGCAAGGTTGAAAGCTCTTCTTCTAGCCTTGCGTGCATTTTCCTTGAGACTTAAAGGAACTAACCCCTTGTAAATCAACAAACGACGTTGGAATAAATGTTATGTGATCCTTATTCATGTAGAGCTGCATGGTTTAAAAATGAACCTTTTACAAAATAACCAACTTGAAAAAGAGATGCCATTGTTCGAATAGCTACAAAAAAAAAAAAATAGCCTATAAATACATATCTCTTTTCAACTATTTTAGTGAACTTTTTTCTATAAGCTTTTTCACCCATAAATTTCTTGATGATATTATTATTAATATAACAATTGTAGCTCACACAATTTTAAATTTTATACTAAATTTCTGAATAATATATTTTTAA

Coding sequence (CDS)

ATGGCCTCGTTATCCACGGTGGTCGGATCTCAACCGGCTATCAAAGTCATCGCTCTCTGTGGTTCTCTTCGTCAAAACTCTACCAACCGAGGCCTTATTCGTGCCGCAACGGAGATAGCGAAGGAATCCATCGACGGATTGCAAATTGAATATTTGGATATCTCCCCATTGCCGATGCTGAATACCGATTTGGAGGTTGATGGAACATATCCGCCCGCCGTTGAGGCGTTTCGCCGGAAGATTCTTGAAGCTGATAGCATTCTCTTTGCATCGCCGGAATATAACTACTCTGTTACCGCACCATTGAAGAATGCAATAGACTGGGGATCAAGGCCACCAAATGTATGGGGAGACAAAGCAGCAGCAATAGTTAGTGCAAGTGGTGGCTCTGGTGGTGTAAGATCACAGTACCATCTTCGCCAAATTGGAGTTTTTCTTGACCTTCATTTCATAAACAAGCCTGAGTTTTTCTTGAATGCACATCAGCCACCCCCCAAGTTCAACAGCAGCGGTGACTTGATCGATGCCGAAGTTCGAGCAAGGTTGAAAGCTCTTCTTCTAGCCTTGCGTGCATTTTCCTTGAGACTTAAAGGAACTAACCCCTTGTAA

Protein sequence

MASLSTVVGSQPAIKVIALCGSLRQNSTNRGLIRAATEIAKESIDGLQIEYLDISPLPMLNTDLEVDGTYPPAVEAFRRKILEADSILFASPEYNYSVTAPLKNAIDWGSRPPNVWGDKAAAIVSASGGSGGVRSQYHLRQIGVFLDLHFINKPEFFLNAHQPPPKFNSSGDLIDAEVRARLKALLLALRAFSLRLKGTNPL

Homology

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BLAST of Cucsat.G10300.T1 vs. ExPASy Swiss-Prot
Match: Q8H9D2 (NAD(P)H:quinone oxidoreductase OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 3.1e-78
Identity = 142/191 (74.35%), Postives = 161/191 (84.29%), Query Frame = 0


Query: 8   VGSQPAIKVIALCGSLRQNSTNRGLIRAATEIAKESIDGLQIEYLDISPLPMLNTDLEVD 67
           + +QP IKV  LCGSLR+ S NRGL+ AA EI K+SI G++IEY+DISPLP LNTDLEV+
Sbjct: 1   MAAQPVIKVAGLCGSLRKGSYNRGLLNAAMEICKDSITGMEIEYVDISPLPFLNTDLEVN 60

Query: 68  GTYPPAVEAFRRKILEADSILFASPEYNYSVTAPLKNAIDWGSRPPNVWGDKAAAIVSAS 127
           GTYPP VEAFR+KI EAD  LFASPEYNYS+T PLKNAIDW SRPPNVW DKAAA+VSA 
Sbjct: 61  GTYPPVVEAFRKKIEEADCFLFASPEYNYSITGPLKNAIDWASRPPNVWADKAAAMVSAG 120

Query: 128 GGSGGVRSQYHLRQIGVFLDLHFINKPEFFLNAHQPPPKFNSSGDLIDAEVRARLKALLL 187
           GG GG RSQYHLRQIGVFLDLHFINKPEFFLNA Q PPKF+S G L D E + RL+A+LL
Sbjct: 121 GGFGGGRSQYHLRQIGVFLDLHFINKPEFFLNAFQQPPKFDSDGVLTDEETKQRLRAVLL 180

Query: 188 ALRAFSLRLKG 199
           AL+A +L+LKG
Sbjct: 181 ALQALALKLKG 191

[Close]

BLAST of Cucsat.G10300.T1 vs. ExPASy Swiss-Prot
Match: Q9LK88 (NADPH:quinone oxidoreductase OS=Arabidopsis thaliana OX=3702 GN=NQR PE=1 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 1.4e-75
Identity = 136/195 (69.74%), Postives = 164/195 (84.10%), Query Frame = 0


Query: 4   LSTVVGSQPAIKVIALCGSLRQNSTNRGLIRAATEIAKESIDGLQIEYLDISPLPMLNTD 63
           +  V   +P I+V AL GSLR+ S + GL+RAA ++ KES+ GLQIEY+DISPLP++NTD
Sbjct: 1   MEAVTAIKPLIRVAALSGSLRKTSFHTGLLRAAIDLTKESVPGLQIEYIDISPLPLINTD 60

Query: 64  LEVDGTYPPAVEAFRRKILEADSILFASPEYNYSVTAPLKNAIDWGSRPPNVWGDKAAAI 123
           LEV+GTYPP VEAFR+KILEADSILFASPEYN+SV+APLKNA+DW SRPPNVW DK AAI
Sbjct: 61  LEVNGTYPPVVEAFRQKILEADSILFASPEYNFSVSAPLKNALDWASRPPNVWADKPAAI 120

Query: 124 VSASGGSGGVRSQYHLRQIGVFLDLHFINKPEFFLNAHQPPPKFNSSGDLIDAEVRARLK 183
           +S  GG GG RSQYHLRQIGVFLDLHFINKPEF LNA QPP KF++ G+L+D   + RLK
Sbjct: 121 ISTGGGFGGGRSQYHLRQIGVFLDLHFINKPEFTLNAFQPPQKFDAEGNLVDEVTKERLK 180

Query: 184 ALLLALRAFSLRLKG 199
            +LL+L+AF+LRL+G
Sbjct: 181 QVLLSLQAFTLRLQG 195

[Close]

BLAST of Cucsat.G10300.T1 vs. ExPASy Swiss-Prot
Match: Q941Y8 (Probable NADPH:quinone oxidoreductase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0954000 PE=2 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 3.1e-70
Identity = 125/189 (66.14%), Postives = 155/189 (82.01%), Query Frame = 0


Query: 10  SQPAIKVIALCGSLRQNSTNRGLIRAATEIAKESIDGLQIEYLDISPLPMLNTDLEVDGT 69
           S  A++V A+ GSLR+ S N GLIRAA EI +ESI G+ I+++DI  LP+LNTD+EVD  
Sbjct: 6   SPKALRVAAISGSLRRGSANTGLIRAAKEICEESIPGMVIDHVDIPDLPLLNTDMEVDDG 65

Query: 70  YPPAVEAFRRKILEADSILFASPEYNYSVTAPLKNAIDWGSRPPNVWGDKAAAIVSASGG 129
           +PPAVEAFR  +  AD  LFASPEYNYS++ PLKNA+DWGSRPPN W D+AAAIVSASGG
Sbjct: 66  FPPAVEAFRASVRAADCFLFASPEYNYSISGPLKNALDWGSRPPNCWADRAAAIVSASGG 125

Query: 130 SGGVRSQYHLRQIGVFLDLHFINKPEFFLNAHQPPPKFNSSGDLIDAEVRARLKALLLAL 189
           SGG RS YH+RQ+GVFLD+HFINKPE F+ AHQPP KF+S G+LID E++  LK +LL+L
Sbjct: 126 SGGSRSMYHIRQVGVFLDIHFINKPEVFIKAHQPPKKFDSDGNLIDPEIKEELKDMLLSL 185

Query: 190 RAFSLRLKG 199
           +AF+LRL+G
Sbjct: 186 QAFALRLQG 194

[Close]

BLAST of Cucsat.G10300.T1 vs. ExPASy Swiss-Prot
Match: Q941Z0 (Probable NADPH:quinone oxidoreductase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0953600 PE=2 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 2.3e-65
Identity = 120/189 (63.49%), Postives = 152/189 (80.42%), Query Frame = 0


Query: 10  SQPAIKVIALCGSLRQNSTNRGLIRAATEIAKESIDGLQIEYLDISPLPMLNTDLE-VDG 69
           ++P I+V A+CGSLR+ S N GL+RAA  + +ESI GL+++++DIS LP+LNTDLE  DG
Sbjct: 6   ARPVIRVAAICGSLRKASYNGGLLRAAAGVCEESIPGLRVDHVDISGLPLLNTDLETADG 65

Query: 70  TYPPAVEAFRRKILEADSILFASPEYNYSVTAPLKNAIDWGSRPPNVWGDKAAAIVSASG 129
            +PPAVEAFR K+ +AD  LF SPEYNYS+  PLKNA+DW SR  N W DK AAIVSA G
Sbjct: 66  GFPPAVEAFRDKVRQADCFLFGSPEYNYSIATPLKNALDWASRGQNCWADKPAAIVSAGG 125

Query: 130 GSGGVRSQYHLRQIGVFLDLHFINKPEFFLNAHQPPPKFNSSGDLIDAEVRARLKALLLA 189
           G GG RSQYHLRQ+GVFLDLHFINKPE  + A + PPKF+S G+LIDA++R R+K +LL+
Sbjct: 126 GFGGGRSQYHLRQVGVFLDLHFINKPELAVKAFEQPPKFDSDGNLIDAQIRERIKQVLLS 185

Query: 190 LRAFSLRLK 198
           L+AF+LRL+
Sbjct: 186 LQAFTLRLQ 194

[Close]

BLAST of Cucsat.G10300.T1 vs. ExPASy Swiss-Prot
Match: Q9I4D4 (NAD(P)H-dependent FMN reductase PA1204 OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=PA1204 PE=1 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 3.6e-34
Identity = 86/187 (45.99%), Postives = 118/187 (63.10%), Query Frame = 0


Query: 14  IKVIALCGSLRQNSTNRGLIRAATEIAKESIDGLQIEYLDISPLPMLNTDLEVDGTYPPA 73
           IKV+ + GSLR  S N   ++ A  +      G+ IE  DIS +P+ N D+   G +PPA
Sbjct: 5   IKVLGISGSLRSGSYNSAALQEAIGLVP---PGMSIELADISGIPLYNEDVYALG-FPPA 64

Query: 74  VEAFRRKILEADSILFASPEYNYSVTAPLKNAIDWGSRPP-NVWGDKAAAIVSASGGS-G 133
           VE FR +I  AD++LFA+PEYNYS+   LKNAIDW SRPP   +  K AAI+ AS G  G
Sbjct: 65  VERFREQIRAADALLFATPEYNYSMAGVLKNAIDWASRPPEQPFSGKPAAILGASAGRFG 124

Query: 134 GVRSQYHLRQIGVFLDLHFINKPEFFLNAHQPPPKFNSSGDLIDAEVRARLKALLLALRA 193
             R+QYHLRQ  VFLD+H +NKPE  +++ Q    F++ G L+D + R  ++  L AL+ 
Sbjct: 125 TARAQYHLRQTLVFLDVHPLNKPEVMISSAQ--NAFDAQGRLLDDKARELIQQQLQALQL 184

Query: 194 FSLRLKG 199
           +  RL+G
Sbjct: 185 WVRRLRG 185

[Close]

BLAST of Cucsat.G10300.T1 vs. NCBI nr
Match: XP_004140288.1 (NAD(P)H:quinone oxidoreductase [Cucumis sativus] >KGN51093.1 hypothetical protein Csa_008941 [Cucumis sativus])

HSP 1 Score: 394 bits (1013), Expect = 2.92e-138
Identity = 201/202 (99.50%), Postives = 201/202 (99.50%), Query Frame = 0


Query: 1   MASLSTVVGSQPAIKVIALCGSLRQNSTNRGLIRAATEIAKESIDGLQIEYLDISPLPML 60
           MASLSTVVGSQPAIKVIALCGSLRQNSTNRGLIRAATEIAKESIDGLQIEYLDISPLPML
Sbjct: 1   MASLSTVVGSQPAIKVIALCGSLRQNSTNRGLIRAATEIAKESIDGLQIEYLDISPLPML 60

Query: 61  NTDLEVDGTYPPAVEAFRRKILEADSILFASPEYNYSVTAPLKNAIDWGSRPPNVWGDKA 120
           NTDLEVDGTYPP VEAFRRKILEADSILFASPEYNYSVTAPLKNAIDWGSRPPNVWGDKA
Sbjct: 61  NTDLEVDGTYPPVVEAFRRKILEADSILFASPEYNYSVTAPLKNAIDWGSRPPNVWGDKA 120

Query: 121 AAIVSASGGSGGVRSQYHLRQIGVFLDLHFINKPEFFLNAHQPPPKFNSSGDLIDAEVRA 180
           AAIVSASGGSGGVRSQYHLRQIGVFLDLHFINKPEFFLNAHQPPPKFNSSGDLIDAEVRA
Sbjct: 121 AAIVSASGGSGGVRSQYHLRQIGVFLDLHFINKPEFFLNAHQPPPKFNSSGDLIDAEVRA 180

Query: 181 RLKALLLALRAFSLRLKGTNPL 202
           RLKALLLALRAFSLRLKGTNPL
Sbjct: 181 RLKALLLALRAFSLRLKGTNPL 202

[Close]

BLAST of Cucsat.G10300.T1 vs. NCBI nr
Match: XP_008465837.1 (PREDICTED: NAD(P)H:quinone oxidoreductase [Cucumis melo])

HSP 1 Score: 373 bits (958), Expect = 6.37e-130
Identity = 189/197 (95.94%), Postives = 195/197 (98.98%), Query Frame = 0


Query: 1   MASLSTVVGSQPAIKVIALCGSLRQNSTNRGLIRAATEIAKESIDGLQIEYLDISPLPML 60
           MASLSTVVGSQPAIKVIALCGSLRQNS+NRGLIRAATEIAKESI+GLQIEYLDISPLPML
Sbjct: 1   MASLSTVVGSQPAIKVIALCGSLRQNSSNRGLIRAATEIAKESIEGLQIEYLDISPLPML 60

Query: 61  NTDLEVDGTYPPAVEAFRRKILEADSILFASPEYNYSVTAPLKNAIDWGSRPPNVWGDKA 120
           NTDLEVDGTYPP VEAFRRKILEADS+LFASPEYNYSVTAPLKNAIDWGSRPPNVWGDKA
Sbjct: 61  NTDLEVDGTYPPVVEAFRRKILEADSVLFASPEYNYSVTAPLKNAIDWGSRPPNVWGDKA 120

Query: 121 AAIVSASGGSGGVRSQYHLRQIGVFLDLHFINKPEFFLNAHQPPPKFNSSGDLIDAEVRA 180
           AAIVSASGGSGGVRSQYHLRQIGVFLDLHFINKPEFFLNAHQPPPKF+SSGDLIDAEVRA
Sbjct: 121 AAIVSASGGSGGVRSQYHLRQIGVFLDLHFINKPEFFLNAHQPPPKFDSSGDLIDAEVRA 180

Query: 181 RLKALLLALRAFSLRLK 197
           +LK+LLLAL AFSLRLK
Sbjct: 181 KLKSLLLALHAFSLRLK 197

[Close]

BLAST of Cucsat.G10300.T1 vs. NCBI nr
Match: XP_038876071.1 (LOW QUALITY PROTEIN: NAD(P)H:quinone oxidoreductase-like [Benincasa hispida])

HSP 1 Score: 369 bits (946), Expect = 4.96e-128
Identity = 186/201 (92.54%), Postives = 192/201 (95.52%), Query Frame = 0


Query: 2   ASLSTVVGSQPAIKVIALCGSLRQNSTNRGLIRAATEIAKESIDGLQIEYLDISPLPMLN 61
           A+LSTV+GSQPAIKVIALCGSLRQNS NRGLIRAA EIAKESIDGLQIEY+DISPLPMLN
Sbjct: 3   AALSTVIGSQPAIKVIALCGSLRQNSYNRGLIRAAMEIAKESIDGLQIEYVDISPLPMLN 62

Query: 62  TDLEVDGTYPPAVEAFRRKILEADSILFASPEYNYSVTAPLKNAIDWGSRPPNVWGDKAA 121
           TDLEVDGTYPP VEAFRRKILEADS LFASPEYNYSVTAPLKNAIDWGSRPPNVWGDKAA
Sbjct: 63  TDLEVDGTYPPVVEAFRRKILEADSFLFASPEYNYSVTAPLKNAIDWGSRPPNVWGDKAA 122

Query: 122 AIVSASGGSGGVRSQYHLRQIGVFLDLHFINKPEFFLNAHQPPPKFNSSGDLIDAEVRAR 181
           AIVSASGGS GVRSQYHL QIGVFLDLHFINKPEFFLNAHQPPPKF+ +GDLIDAEVR +
Sbjct: 123 AIVSASGGSSGVRSQYHLXQIGVFLDLHFINKPEFFLNAHQPPPKFDGNGDLIDAEVREK 182

Query: 182 LKALLLALRAFSLRLKGTNPL 202
           LKALLLALRAFSLRLKGT PL
Sbjct: 183 LKALLLALRAFSLRLKGTKPL 203

[Close]

BLAST of Cucsat.G10300.T1 vs. NCBI nr
Match: XP_038906719.1 (NAD(P)H:quinone oxidoreductase-like [Benincasa hispida])

HSP 1 Score: 363 bits (931), Expect = 8.25e-125
Identity = 183/200 (91.50%), Postives = 190/200 (95.00%), Query Frame = 0


Query: 2   ASLSTVVGSQPAIKVIALCGSLRQNSTNRGLIRAATEIAKESIDGLQIEYLDISPLPMLN 61
           A+LSTVVGSQPAIKVIALCGSLRQNS NRGLIRAATEIAKESIDGL IE++DISPLPMLN
Sbjct: 61  AALSTVVGSQPAIKVIALCGSLRQNSYNRGLIRAATEIAKESIDGLHIEFIDISPLPMLN 120

Query: 62  TDLEVDGTYPPAVEAFRRKILEADSILFASPEYNYSVTAPLKNAIDWGSRPPNVWGDKAA 121
           TDLEVDGTYPP VEAFRRKILEADS LFASPEYNYS+TAPLKNAIDWGSRPPNVW DKAA
Sbjct: 121 TDLEVDGTYPPVVEAFRRKILEADSYLFASPEYNYSITAPLKNAIDWGSRPPNVWRDKAA 180

Query: 122 AIVSASGGSGGVRSQYHLRQIGVFLDLHFINKPEFFLNAHQPPPKFNSSGDLIDAEVRAR 181
           AIVSA GGSGG+RSQYHLRQ+GVFLDLHFINKPEFFLNA Q PPKF S+GDLIDAEVR R
Sbjct: 181 AIVSAGGGSGGLRSQYHLRQVGVFLDLHFINKPEFFLNAFQTPPKFGSNGDLIDAEVRER 240

Query: 182 LKALLLALRAFSLRLKGTNP 201
           LKALLLALRAFSLRLKGTNP
Sbjct: 241 LKALLLALRAFSLRLKGTNP 260

[Close]

BLAST of Cucsat.G10300.T1 vs. NCBI nr
Match: XP_022146336.1 (NAD(P)H:quinone oxidoreductase-like [Momordica charantia])

HSP 1 Score: 347 bits (889), Expect = 2.33e-119
Identity = 170/200 (85.00%), Postives = 191/200 (95.50%), Query Frame = 0


Query: 1   MASLSTVVGSQPAIKVIALCGSLRQNSTNRGLIRAATEIAKESIDGLQIEYLDISPLPML 60
           MA+LSTVVG+QPAIK++ALCGSLRQNS NRGLIRAA EIA+ESI GL+IEY+DISPLPML
Sbjct: 1   MAALSTVVGAQPAIKIVALCGSLRQNSYNRGLIRAAAEIAEESIKGLEIEYVDISPLPML 60

Query: 61  NTDLEVDGTYPPAVEAFRRKILEADSILFASPEYNYSVTAPLKNAIDWGSRPPNVWGDKA 120
           NTDLE+DGT+PPAVEAFRRKILEADSILFASPEYNYSVTAPLKNA+DWGSRPPNVWGDKA
Sbjct: 61  NTDLEIDGTFPPAVEAFRRKILEADSILFASPEYNYSVTAPLKNALDWGSRPPNVWGDKA 120

Query: 121 AAIVSASGGSGGVRSQYHLRQIGVFLDLHFINKPEFFLNAHQPPPKFNSSGDLIDAEVRA 180
           AA+VSASGGS GVRSQYHLRQIGVFLDLHFINKPEFFLNA QPPPKF+++GDLIDA++R 
Sbjct: 121 AAVVSASGGSSGVRSQYHLRQIGVFLDLHFINKPEFFLNAFQPPPKFDANGDLIDAQIRD 180

Query: 181 RLKALLLALRAFSLRLKGTN 200
           +LKALLL+L+AF+ RL+ T 
Sbjct: 181 KLKALLLSLQAFASRLRPTR 200

[Close]

BLAST of Cucsat.G10300.T1 vs. ExPASy TrEMBL
Match: A0A0A0KNE1 (NAD(P)H dehydrogenase (quinone) OS=Cucumis sativus OX=3659 GN=Csa_5G435080 PE=4 SV=1)

HSP 1 Score: 394 bits (1013), Expect = 1.41e-138
Identity = 201/202 (99.50%), Postives = 201/202 (99.50%), Query Frame = 0


Query: 1   MASLSTVVGSQPAIKVIALCGSLRQNSTNRGLIRAATEIAKESIDGLQIEYLDISPLPML 60
           MASLSTVVGSQPAIKVIALCGSLRQNSTNRGLIRAATEIAKESIDGLQIEYLDISPLPML
Sbjct: 1   MASLSTVVGSQPAIKVIALCGSLRQNSTNRGLIRAATEIAKESIDGLQIEYLDISPLPML 60

Query: 61  NTDLEVDGTYPPAVEAFRRKILEADSILFASPEYNYSVTAPLKNAIDWGSRPPNVWGDKA 120
           NTDLEVDGTYPP VEAFRRKILEADSILFASPEYNYSVTAPLKNAIDWGSRPPNVWGDKA
Sbjct: 61  NTDLEVDGTYPPVVEAFRRKILEADSILFASPEYNYSVTAPLKNAIDWGSRPPNVWGDKA 120

Query: 121 AAIVSASGGSGGVRSQYHLRQIGVFLDLHFINKPEFFLNAHQPPPKFNSSGDLIDAEVRA 180
           AAIVSASGGSGGVRSQYHLRQIGVFLDLHFINKPEFFLNAHQPPPKFNSSGDLIDAEVRA
Sbjct: 121 AAIVSASGGSGGVRSQYHLRQIGVFLDLHFINKPEFFLNAHQPPPKFNSSGDLIDAEVRA 180

Query: 181 RLKALLLALRAFSLRLKGTNPL 202
           RLKALLLALRAFSLRLKGTNPL
Sbjct: 181 RLKALLLALRAFSLRLKGTNPL 202

[Close]

BLAST of Cucsat.G10300.T1 vs. ExPASy TrEMBL
Match: A0A1S3CPU3 (NAD(P)H dehydrogenase (quinone) OS=Cucumis melo OX=3656 GN=LOC103503429 PE=4 SV=1)

HSP 1 Score: 373 bits (958), Expect = 3.09e-130
Identity = 189/197 (95.94%), Postives = 195/197 (98.98%), Query Frame = 0


Query: 1   MASLSTVVGSQPAIKVIALCGSLRQNSTNRGLIRAATEIAKESIDGLQIEYLDISPLPML 60
           MASLSTVVGSQPAIKVIALCGSLRQNS+NRGLIRAATEIAKESI+GLQIEYLDISPLPML
Sbjct: 1   MASLSTVVGSQPAIKVIALCGSLRQNSSNRGLIRAATEIAKESIEGLQIEYLDISPLPML 60

Query: 61  NTDLEVDGTYPPAVEAFRRKILEADSILFASPEYNYSVTAPLKNAIDWGSRPPNVWGDKA 120
           NTDLEVDGTYPP VEAFRRKILEADS+LFASPEYNYSVTAPLKNAIDWGSRPPNVWGDKA
Sbjct: 61  NTDLEVDGTYPPVVEAFRRKILEADSVLFASPEYNYSVTAPLKNAIDWGSRPPNVWGDKA 120

Query: 121 AAIVSASGGSGGVRSQYHLRQIGVFLDLHFINKPEFFLNAHQPPPKFNSSGDLIDAEVRA 180
           AAIVSASGGSGGVRSQYHLRQIGVFLDLHFINKPEFFLNAHQPPPKF+SSGDLIDAEVRA
Sbjct: 121 AAIVSASGGSGGVRSQYHLRQIGVFLDLHFINKPEFFLNAHQPPPKFDSSGDLIDAEVRA 180

Query: 181 RLKALLLALRAFSLRLK 197
           +LK+LLLAL AFSLRLK
Sbjct: 181 KLKSLLLALHAFSLRLK 197

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BLAST of Cucsat.G10300.T1 vs. ExPASy TrEMBL
Match: A0A6J1CZ25 (NAD(P)H dehydrogenase (quinone) OS=Momordica charantia OX=3673 GN=LOC111015573 PE=4 SV=1)

HSP 1 Score: 347 bits (889), Expect = 1.13e-119
Identity = 170/200 (85.00%), Postives = 191/200 (95.50%), Query Frame = 0


Query: 1   MASLSTVVGSQPAIKVIALCGSLRQNSTNRGLIRAATEIAKESIDGLQIEYLDISPLPML 60
           MA+LSTVVG+QPAIK++ALCGSLRQNS NRGLIRAA EIA+ESI GL+IEY+DISPLPML
Sbjct: 1   MAALSTVVGAQPAIKIVALCGSLRQNSYNRGLIRAAAEIAEESIKGLEIEYVDISPLPML 60

Query: 61  NTDLEVDGTYPPAVEAFRRKILEADSILFASPEYNYSVTAPLKNAIDWGSRPPNVWGDKA 120
           NTDLE+DGT+PPAVEAFRRKILEADSILFASPEYNYSVTAPLKNA+DWGSRPPNVWGDKA
Sbjct: 61  NTDLEIDGTFPPAVEAFRRKILEADSILFASPEYNYSVTAPLKNALDWGSRPPNVWGDKA 120

Query: 121 AAIVSASGGSGGVRSQYHLRQIGVFLDLHFINKPEFFLNAHQPPPKFNSSGDLIDAEVRA 180
           AA+VSASGGS GVRSQYHLRQIGVFLDLHFINKPEFFLNA QPPPKF+++GDLIDA++R 
Sbjct: 121 AAVVSASGGSSGVRSQYHLRQIGVFLDLHFINKPEFFLNAFQPPPKFDANGDLIDAQIRD 180

Query: 181 RLKALLLALRAFSLRLKGTN 200
           +LKALLL+L+AF+ RL+ T 
Sbjct: 181 KLKALLLSLQAFASRLRPTR 200

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BLAST of Cucsat.G10300.T1 vs. ExPASy TrEMBL
Match: A0A6J1E6Y2 (NAD(P)H dehydrogenase (quinone) OS=Cucurbita moschata OX=3662 GN=LOC111429982 PE=4 SV=1)

HSP 1 Score: 341 bits (874), Expect = 1.83e-117
Identity = 166/194 (85.57%), Postives = 185/194 (95.36%), Query Frame = 0


Query: 7   VVGSQPAIKVIALCGSLRQNSTNRGLIRAATEIAKESIDGLQIEYLDISPLPMLNTDLEV 66
           + G+QPA+KV+ALCGSLR+NSTNRGLIRAA EIAKESIDG++IEY+DISPLPMLNTDLEV
Sbjct: 1   MAGAQPAVKVVALCGSLRKNSTNRGLIRAAIEIAKESIDGMEIEYVDISPLPMLNTDLEV 60

Query: 67  DGTYPPAVEAFRRKILEADSILFASPEYNYSVTAPLKNAIDWGSRPPNVWGDKAAAIVSA 126
           DGTYPPAVEAFRRKILEA+S+LFASPEYNYSVTAPLKNA+DW SRPPNVWG+KAAAIVS+
Sbjct: 61  DGTYPPAVEAFRRKILEAESVLFASPEYNYSVTAPLKNALDWASRPPNVWGNKAAAIVSS 120

Query: 127 SGGSGGVRSQYHLRQIGVFLDLHFINKPEFFLNAHQPPPKFNSSGDLIDAEVRARLKALL 186
           SGGS G RSQYHLRQIGVFLDLHFINKPEFFLNA+QPPPKF+S GDLID EVR +LKA+L
Sbjct: 121 SGGSRGARSQYHLRQIGVFLDLHFINKPEFFLNAYQPPPKFDSHGDLIDREVREKLKAVL 180

Query: 187 LALRAFSLRLKGTN 200
           LAL AFSLRL+GT+
Sbjct: 181 LALHAFSLRLRGTS 194

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BLAST of Cucsat.G10300.T1 vs. ExPASy TrEMBL
Match: A0A6J1JQQ6 (NAD(P)H dehydrogenase (quinone) OS=Cucurbita maxima OX=3661 GN=LOC111487488 PE=4 SV=1)

HSP 1 Score: 340 bits (873), Expect = 3.10e-117
Identity = 168/198 (84.85%), Postives = 187/198 (94.44%), Query Frame = 0


Query: 3   SLSTVVGSQPAIKVIALCGSLRQNSTNRGLIRAATEIAKESIDGLQIEYLDISPLPMLNT 62
           +LS +VG+QPA+KV+ALCGSLR++STNRGLIRAATEIAKESIDGL+IEY+DISPLPMLNT
Sbjct: 2   ALSMIVGAQPAVKVVALCGSLRKSSTNRGLIRAATEIAKESIDGLEIEYVDISPLPMLNT 61

Query: 63  DLEVDGTYPPAVEAFRRKILEADSILFASPEYNYSVTAPLKNAIDWGSRPPNVWGDKAAA 122
           DLEVDGTY PAVEAFRRKILEA+SILFASPEYNYSVTAPLKNA+DW SRPPNVWGDKAAA
Sbjct: 62  DLEVDGTYSPAVEAFRRKILEAESILFASPEYNYSVTAPLKNALDWASRPPNVWGDKAAA 121

Query: 123 IVSASGGSGGVRSQYHLRQIGVFLDLHFINKPEFFLNAHQPPPKFNSSGDLIDAEVRARL 182
           IVS+SG  GG RSQYHLRQIGVFLDLHFINKPEFFLNA+QPPPKF+S GDLID +VR +L
Sbjct: 122 IVSSSGSLGGARSQYHLRQIGVFLDLHFINKPEFFLNAYQPPPKFDSHGDLIDCKVREKL 181

Query: 183 KALLLALRAFSLRLKGTN 200
           KA+LLAL AFSLRL+ T+
Sbjct: 182 KAVLLALHAFSLRLRRTS 199

The following BLAST results are available for this feature:

BLAST of Cucsat.G10300.T1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8H9D2	3.1e-78	74.35	NAD(P)H:quinone oxidoreductase OS=Solanum tuberosum OX=4113 PE=2 SV=1	[more]
Q9LK88	1.4e-75	69.74	NADPH:quinone oxidoreductase OS=Arabidopsis thaliana OX=3702 GN=NQR PE=1 SV=1	[more]
Q941Y8	3.1e-70	66.14	Probable NADPH:quinone oxidoreductase 2 OS=Oryza sativa subsp. japonica OX=39947...	[more]
Q941Z0	2.3e-65	63.49	Probable NADPH:quinone oxidoreductase 1 OS=Oryza sativa subsp. japonica OX=39947...	[more]
Q9I4D4	3.6e-34	45.99	NAD(P)H-dependent FMN reductase PA1204 OS=Pseudomonas aeruginosa (strain ATCC 15...	[more]

BLAST of Cucsat.G10300.T1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004140288.1	2.92e-138	99.50	NAD(P)H:quinone oxidoreductase [Cucumis sativus] >KGN51093.1 hypothetical protei...	[more]
XP_008465837.1	6.37e-130	95.94	PREDICTED: NAD(P)H:quinone oxidoreductase [Cucumis melo]	[more]
XP_038876071.1	4.96e-128	92.54	LOW QUALITY PROTEIN: NAD(P)H:quinone oxidoreductase-like [Benincasa hispida]	[more]
XP_038906719.1	8.25e-125	91.50	NAD(P)H:quinone oxidoreductase-like [Benincasa hispida]	[more]
XP_022146336.1	2.33e-119	85.00	NAD(P)H:quinone oxidoreductase-like [Momordica charantia]	[more]

BLAST of Cucsat.G10300.T1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KNE1	1.41e-138	99.50	NAD(P)H dehydrogenase (quinone) OS=Cucumis sativus OX=3659 GN=Csa_5G435080 PE=4 ...	[more]
A0A1S3CPU3	3.09e-130	95.94	NAD(P)H dehydrogenase (quinone) OS=Cucumis melo OX=3656 GN=LOC103503429 PE=4 SV=...	[more]
A0A6J1CZ25	1.13e-119	85.00	NAD(P)H dehydrogenase (quinone) OS=Momordica charantia OX=3673 GN=LOC111015573 P...	[more]
A0A6J1E6Y2	1.83e-117	85.57	NAD(P)H dehydrogenase (quinone) OS=Cucurbita moschata OX=3662 GN=LOC111429982 PE...	[more]
A0A6J1JQQ6	3.10e-117	84.85	NAD(P)H dehydrogenase (quinone) OS=Cucurbita maxima OX=3661 GN=LOC111487488 PE=4...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029039	Flavoprotein-like superfamily	GENE3D	3.40.50.360		coord: 3..201 e-value: 3.7E-55 score: 188.8
IPR029039	Flavoprotein-like superfamily	SUPERFAMILY	52218	Flavoproteins	coord: 15..186
IPR005025	NADPH-dependent FMN reductase-like	PFAM	PF03358	FMN_red	coord: 15..159 e-value: 1.9E-37 score: 128.4
None	No IPR available	PANTHER	PTHR30543:SF22	FLAVOPROTEIN-LIKE DOMAIN-CONTAINING PROTEIN-RELATED	coord: 8..198
None	No IPR available	PANTHER	PTHR30543	CHROMATE REDUCTASE	coord: 8..198

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G10300	Cucsat.G10300	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G10300.T1.E1	Cucsat.G10300.T1.E1	exon
Cucsat.G10300.T1.E2	Cucsat.G10300.T1.E2	exon
Cucsat.G10300.T1.E3	Cucsat.G10300.T1.E3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G10300.T1.C1	Cucsat.G10300.T1.C1	CDS
Cucsat.G10300.T1.C2	Cucsat.G10300.T1.C2	CDS
Cucsat.G10300.T1.C3	Cucsat.G10300.T1.C3	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G10300.T1	Cucsat.G10300.T1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005829	cytosol
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0010181	FMN binding
molecular_function	GO:0050136	NADH dehydrogenase (quinone) activity
molecular_function	GO:0008753	NADPH dehydrogenase (quinone) activity