Cucsat.G10112.T12 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G10112.T12
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	protease Do-like 1, chloroplastic
Location	ctg1673: 5554839 .. 5560440 (+)
RNA-Seq Expression	Cucsat.G10112.T12
Synteny	Cucsat.G10112.T12

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TGGTTGAAATTGGATTTCATTCATCGATTCAATTTTCAGCAACTGTCCAACAAACCCAAAACAAATGGCTGCTGCTGCTGCATTTTCTCTGCTGCCTTCTTCACTTTCTCGTCCTCCTAAAAGACCACCCACTTCCTTTTTTCTCTCAAAGTCCATTTCTTTTCACAATTTTTCCAATCCCACCCGCCATTTTAGATACCCCATCTTCTCTCTTCTTCACCACAACAAGAAACACTCCCAATTTTCTCCTTCAATTCTTCCAAAACTCCCCGTTCCCTCCAACCCCTTTGCTTCAATTTGCGAATCGCTTCTTGTTTTCTCCACTTCGGTGCTCTTGTCCTTTGCTCTTTTTGTAACAGATGTTGATCCGGCGGTTGCTTTTGTTGTCACTACGCCGAGGAAGTTGCAGACGGATGAGCTCGCTACAGTTCGTCTTTTTCAGGAGAATACTCCTTCTGTTGTTTATATCACGAATCTTGCTGTTAGGTAATTGTATTATTATACCCTTTTGGAGCTGACTTCTGATTACAGTTTTTTCTTTTTTTTCTCAATATAAGAAATATTTCATTGAAAGAATGATGTTAGCGCCTTAGCGGAGACCCTAGGCACCACTAATGATTTCCGATCAGAAACTAAGAAAGAGTGAAACTCTTGAGAGTGTGCCTGGTATTGCACCAGTACAACGCAGTAGATAGAACCAAATTCATTAAACTATCGAAACAAGTAGAGGAATTTCTAAAGAGATGCTTATTATGTTTGCCCCATAGTACGCAATATGGCCAACCAATGATTTTATGTAAATTTTAGGCAGGATGCTTTTACTTTAGACGTGCTGGAGGTACCACAGGGGTCCGGTTCTGGATTTGTGTGGGATAAGGATGGTCATATTGTCACCAATTATCATGTTATTCGTGGTGCATCTGATCTCAGGTTAGTTATGAACCCTTTTCCGTGGTTTGCAGTTTTTGGGTTTAATTTTGTTTAGACTAAGAGTTAATTTATTTGTCATTTTAGTATTTGGACCTTCCTCAGCTTGTATTGATTTGTATGTTCATTACCTTGGGGGCTTTATCCATGTAATGCTGTGTTATTACTTCTAGTATGTATAGCCTGTTCATACCCATTGAAGCATCATCGAAGCACTTAATTGTAGAACCACCATGTCGCAAATGGCCCTCAGAAAATGTTTTTAGGCTTTATATTTTCAGATATGTGCAGCCACATAGGCAGAACTGTATCATATTTTTGGCTATGTGATTTTCTTTTATAATAACCTCTGGAATTATCGGTAAATATTTTCGACCAAAACAAATATGAATTATTTGTCTTGGTTATGTGGTAATTCACACAGCCATACTTTTTTTGCTATGTGAATTATCAGGAAGGAGATATCAGGGCATTATCAGTAAATATATTTGTTTTAAAATGCAGAAATTTTAAATTTTCCACCTTCCCTTGAGGTCAAAGGGGAAATTTTGGAACACTTTCAAGAACTCTACTCCAAATTTCTTGGCCTAAGACTGTCTTCAATGTTTGGATTGACAATTAGCTAATTAGTCCAGAAAACCATGGTTGTCGATTCCCCATAAAGACCAATTGGCTTTTTCTTTCTTCTGTGAGTGTTGGAGTTAGCTTGTTCGCTTCTTGGATATTCTCATATTCATAGGACAATTGCTTGGCCCTACTATATTTGGTTGTCGAGAAACTCATAGGATGTTAAGTCGTAAGTAAGTCACCATTGTTCAAACCCATTTCTTTGGAGGCTTTTTTTTTTTTTATTTTGATGCCATATATTGACCTTGAAGTCAGCCGGTGGTGGTTAGCCAAGTGGAGTTTCGATGTTTGTTTTGAGGGAAATTTGGAGTTTTATGAGTAAATTAGGCTTTCTCAAGTTTTATGTCTTTCCCAAATCAACTAGAGTGTTTTCTAAAACTCTTTTTGCCTCAGCAAAATGCAAAAGAAGGTACTATCAAGTTCTTTTATACTTACAATGAGCTAGAATTGGATTGACATTAGACTTAAGACTTAGCAGTGGCTACTTATTATTCGCATTTAATCTGGTTTTAGTTATGTACAGAGTCACTCTAGCTGACCAAACTACTTTCGATGCGAAAGTTGTTGGATTTGACCAAGACAAGGATGTTGCTGTCTTGAGTATCGATGCACCTAAAGACAAACTAAGACCTATACCTGTTGGTATATCAGCAGATTTGCTTGTTGGTCAAAAGGTGTTTGCTATTGGAAACCCTGTAAGTTTTTCTATTCATTGCCCTTTTTCCCTGGGTGTATGTGCACATTATCATATCCATTCATCTTTTATATCAAAGTTCATGCTATTGATTTCTTAAAACAAAAATGAAAAATCTAGGATGAAGCGATCTCTGGAAAGAGGATAATCTCTTAAAATGTTTTCAAAAGAGGAAAACCCCAGATTAATTTTGCGTTAAATGGACAATCCTTATATGCTATAGTTTTATCTACAAAATCAATCGGGGGAATGAACTCATTGTTTAGATTAACTAGGAAAAAAATTGTTTTAAAATTTTTGTATGTAAATCTTTTTTTGATAAAGACAACTATTTCAAAAGCTATTTTGAGTAGTTGTTAAACACTTCTATTTTTTCTAAAATGTCTTAGTTTTAAAATAAAATTCTTGAAATGTTAAACCGACCATAGTCTTAGTTGGGAGATGATCACCCCTTTTTTCATTTTCCCTGAAGCCAGTCTCTTCATTTGTTATCTTGTACATTTCCCCATTTGATGAACTAAAAATATAGTAATTTTCGTCTGTGCAGTTTGGACTGGATCATACTCTTACAACTGGTGTAATCAGGTATGAAACCCACAAGTTTTAATGGATTATATTCCTCACAAATTCTTTAGAAATTTACATTTTAATTTAGTATGTTAATCTTATACTATAATTGAGCCCCCCAAAAATTATTAGAGATTGTAATTTCAATTAACAATAATCACGAAGATGATGTTTGTTTTAGAGTTGAAATAATTCTGTTTATATATGTCTCCTTTTTACTTTTCCTAAAATTGTTTATTTTCTTCATGATCCTTCAAATGCAAAATGGGTTTATCCTTATGAGACATGTTATATTTTTACTATTTGGTTGCGGTCGAAACCTGTATTCATAGGCTTTGAAACTAGGGGTGATCATCAATGGATCGACCAGCAGACAATAGTTGGGTTTCCAGAAAATTTCGAAATTTTTTGGAAATCCAACATTCTCACTGAATTGCCTCTCTTACTCCCTCTGACCAACCAACTTGGCCGGTCATCTTGGTTTTGCGGTCTATCATGCTCACCCTATTTGAAACTGTAATACTAAACATTCAACTTGCCCTTGACAGTGGCCTTCGGAGAGAAATTAGTTCTGCGGCTACTGGTAGGCCAATTCAAGATGTCATACAGACGGATGCTGCCATTAATCCAGGAAACAGTGGAGGGCCACTTCTCGATAGTTCGGGGAACCTTATTGGAATTAATACGGCAATATATTCTCCATCTGGGGCCTCCTCTGGTGTTGGATTTTCAATTCCTGTTGACACAGTTAGTACACATGACATATAATTAACAAATTGAAGTTCCCTTAGTTTGATATTTGAAGTACATGATCCATTAACATTCTGTCAATTTAGGTGAGTGGTATTGTTGATCAGTTGGTGCGATTTGGGAAGGTGACAAGACCAATTTTAGGAATCAAGTTTGCTCCCGATCAGTCTGTCGAACAGTTGGGTGTAAGCGGAGTGCTTGTCTTAGATGCTCCTGCTAATGGTCCTGCTGGGAAAGCTGTACTCATTTCCTCTCTTTTCCTATCTGCATTCATATTCTTATATTCCTATAGGTTTCAATATTCCATTATGTTTACAAGAACTTATGAACGTCCCCCAACCTTAATACTTCGGTTCAAGAAGATCTAATCGCTTGACATTATGCAGGGCCTGCTACCGACTAAGCGCGATGCTTATGGTAGACTTATATTGGGAGACATAATAACATCAGTGAATGGCAAAAAGGTCACAAATGGAAGCGATTTGTATAGAATTCTTGACCAATGTAAAGTTGGTGATAAGGTAGCCTTCATTTCCCTGTTTGATCTTTTTACTTCAGCTTGTTTGAACTTTTTAACTTTTGAAGAAAGGCATCATACTGCTTTTATTTTAAGATGTTGGGAAGTAATTTAAAACTTTGGTTCATGCTTGAGTGGATCCATAATAAAGTTAGTAAATAGGCTGGTTTGATTGAATCTGTTGGATCCACGCACTGTTTGCCATTGTGTGCCATTGAATTCTCTGTGTCACGTAGTCATTTTTTTTTCAAAGAACTAAGAAGGATTTAAGACATGTTTGGAGATTTTGAAGTAGTCAAAATCATTTTTGTCATGTTGAAAATCACTTACAACAGTGCTAAGTTAATATAATTTAATTTTTACATTTTAAAACCCGATTTTCATACCGGGTAAAAGTAAATATTTTATCAACCTTTCTTTCTTATAGAATTATCTATGGGGTGTTTCTTCCTATAGTTTTGAAGTTTCTTATTGTATAGTATCTTAAAAGTTGAAATATCTTTTCATGTAGCATCATCGATCATGTGCCTTTTCAAAATTCATTTCACTAAATATTATAATCCGCATTTATGCATCAGTGGGAATTATTAAATGGTTCTATCCACTTGAACTAATGAATATTCTTCAAAACTTTGAGTTATGAAAAAGACAGCCAGATCCCATGTGATAAAATCCCATTGCCATCATATATATTGCCTCATAATGCTGAACAGTAGCATAGCATGTGGCTTTTAGTGACATTACACAGCCACAAAACCTTTGAATCGGCCTTTTTTTACTTGGGACAAAGAAGAACGAAGTTTTAATCCTATTTTTTTCATTAACTCCCTGCTTTCTTTTATTTTGATCCTCAAACTTTCAAAAACCGTATTTTAACTCATAAACTTTTAAGCATTCAATAAACAAGTTCCGATTACAAATATAACATTTAAACTCAAAATATTAACCGAGGTAACACATTTTCAAAGCATTTGTAGATATTGTATAGCAAACTTTAGATCCATCTCTATTACCAACAAGAGTTCTATCAACAATAGAGTTTATTACTTGTAGATTTTGTTATATTTACAATTCTTTGAACATGTTAGTGTGTGCTAAGTTGTTAACCCTAAAAGTTGTATGTAGTGGTGATGCTAAGAAAAGGAAATATTTGCATGAATCTGCGGTATAGCAGAACAGGGTATGATGATAAGTAACAATTTGTTTGATGATATAGGTAACTGTGGAGGTACTACGTGGTGATCACATGGAGAAAATTCCAGTAATTCTGGAGCCAAAGCCTGACGAATCTTGAGCCAAACATAGTTGGGGGGGTTTGTCTTGGTTTTTTCTTCTCTCTTTTTTGTACATTACATTAATCAATATTAACAAGCTGTTTGTAAATAGTCTATTCTGGCTGCCACTCTGGCCCTTTTCTCAACTAATCACACTTCAAAAGAAGATTAAATTACAAGTTTTTTAAGTTGTATTTAATAGATCCTTCAATTTTAAAAATTATAGTTTCATAAGCTTCTCCATTT

Coding sequence (CDS)

ATGGCTGCTGCTGCTGCATTTTCTCTGCTGCCTTCTTCACTTTCTCGTCCTCCTAAAAGACCACCCACTTCCTTTTTTCTCTCAAAGTCCATTTCTTTTCACAATTTTTCCAATCCCACCCGCCATTTTAGATACCCCATCTTCTCTCTTCTTCACCACAACAAGAAACACTCCCAATTTTCTCCTTCAATTCTTCCAAAACTCCCCGTTCCCTCCAACCCCTTTGCTTCAATTTGCGAATCGCTTCTTGTTTTCTCCACTTCGGTGCTCTTGTCCTTTGCTCTTTTTGTAACAGATGTTGATCCGGCGGTTGCTTTTGTTGTCACTACGCCGAGGAAGTTGCAGACGGATGAGCTCGCTACAGTTCGTCTTTTTCAGGAGAATACTCCTTCTGTTGTTTATATCACGAATCTTGCTGTTAGAGTCACTCTAGCTGACCAAACTACTTTCGATGCGAAAGTTGTTGGATTTGACCAAGACAAGGATGTTGCTGTCTTGAGTATCGATGCACCTAAAGACAAACTAAGACCTATACCTGTTGGTATATCAGCAGATTTGCTTGTTGGTCAAAAGGTGTTTGCTATTGGAAACCCTTTTGGACTGGATCATACTCTTACAACTGGTGTAATCAGTGGCCTTCGGAGAGAAATTAGTTCTGCGGCTACTGGTAGGCCAATTCAAGATGTCATACAGACGGATGCTGCCATTAATCCAGGAAACAGTGGAGGGCCACTTCTCGATAGTTCGGGGAACCTTATTGGAATTAATACGGCAATATATTCTCCATCTGGGGCCTCCTCTGGTGTTGGATTTTCAATTCCTGTTGACACAGTGAGTGGTATTGTTGATCAGTTGGTGCGATTTGGGAAGGTGACAAGACCAATTTTAGGAATCAAGTTTGCTCCCGATCAGTCTGTCGAACAGTTGGGTGTAAGCGGAGTGCTTGTCTTAGATGCTCCTGCTAATGGTCCTGCTGGGAAAGCTGGCCTGCTACCGACTAAGCGCGATGCTTATGGTAGACTTATATTGGGAGACATAATAACATCAGTGAATGGCAAAAAGGTCACAAATGGAAGCGATTTGTATAGAATTCTTGACCAATGTAAAGTTGGTGATAAGGTAACTGTGGAGGTACTACGTGGTGATCACATGGAGAAAATTCCAGTAATTCTGGAGCCAAAGCCTGACGAATCTTGA

Protein sequence

MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNPTRHFRYPIFSLLHHNKKHSQFSPSILPKLPVPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELATVRLFQENTPSVVYITNLAVRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAIYSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVLRGDHMEKIPVILEPKPDES

Homology

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BLAST of Cucsat.G10112.T12 vs. ExPASy Swiss-Prot
Match: O22609 (Protease Do-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP1 PE=1 SV=2)

HSP 1 Score: 526.6 bits (1355), Expect = 2.5e-148
Identity = 313/446 (70.18%), Postives = 328/446 (73.54%), Query Frame = 0


Query: 1   MAAAAAFSLLPSS---LSRPPKRPPTSFFLSKSISFHNFSNPTRHFRY-PIFSLLHHNKK 60
           MA   + SLL SS   L  PP    + F LS S S      P R  RY  I S L  N  
Sbjct: 1   MATTTSCSLLLSSTLFLHSPPSSHLSFFNLSSSRSSPISLYPIRSKRYFRILSKLSLNDN 60

Query: 61  HSQFSPSILPKLPVPS-NPFASICESLLVFSTSVLLSFALFVTD--VDPAVAFVVTTPRK 120
           +       L   P  +  PF  +C       TSV LSF+LF     V+ A AFVV+TP+K
Sbjct: 61  NRDDDDDTLHFTPFSAVKPFFLLC-------TSVALSFSLFAASPAVESASAFVVSTPKK 120

Query: 121 LQTDELATVRLFQENTPSVVYITNLAV--------------------------------- 180
           LQTDELATVRLFQENTPSVVYITNLAV                                 
Sbjct: 121 LQTDELATVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDKQGHIVTNYH 180

Query: 181 --------RVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKV 240
                   RVTLADQTTFDAKVVGFDQDKDVAVL IDAPK+KLRPIPVG+SADLLVGQKV
Sbjct: 181 VIRGASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKNKLRPIPVGVSADLLVGQKV 240

Query: 241 FAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNL 300
           FAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSG L
Sbjct: 241 FAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGTL 300

Query: 301 IGINTAIYSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVS 360
           IGINTAIYSPSGASSGVGFSIPVDTV GIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVS
Sbjct: 301 IGINTAIYSPSGASSGVGFSIPVDTVGGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVS 360

Query: 361 GVLVLDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGD 399
           GVLVLDAP +GPAGKAGL  TKRD YGRL+LGDIITSVNG KV+NGSDLYRILDQCKVGD
Sbjct: 361 GVLVLDAPPSGPAGKAGLQSTKRDGYGRLVLGDIITSVNGTKVSNGSDLYRILDQCKVGD 420

[Close]

BLAST of Cucsat.G10112.T12 vs. ExPASy Swiss-Prot
Match: Q9LU10 (Protease Do-like 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP8 PE=1 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 3.1e-66
Identity = 140/249 (56.22%), Postives = 174/249 (69.88%), Query Frame = 0


Query: 147 QTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPFGLDHTLT 206
           Q  F+ K+VG D+ KD+AVL +DAP+  L+PI VG S  L VGQ+  AIGNPFG DHTLT
Sbjct: 199 QKNFEGKLVGADRAKDLAVLKVDAPETLLKPIKVGQSNSLKVGQQCLAIGNPFGFDHTLT 258

Query: 207 TGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAIYSPSGAS 266
            GVISGL R+I S  TG  I   IQTDAAINPGNSGGPLLDS GNLIGINTAI++ +G S
Sbjct: 259 VGVISGLNRDIFS-QTGVTIGGGIQTDAAINPGNSGGPLLDSKGNLIGINTAIFTQTGTS 318

Query: 267 SGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGV-SGVLVLDAPANGPA 326
           +GVGF+IP  TV  IV QL++F KV R  + I+ APD    QL V +G LVL  P    A
Sbjct: 319 AGVGFAIPSSTVLKIVPQLIQFSKVLRAGINIELAPDPVANQLNVRNGALVLQVPGKSLA 378

Query: 327 GKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVLRGDHME 386
            KAGL PT R   G ++LGDII +V+ K V N ++L +ILD+  VGDKVT+++ RG+   
Sbjct: 379 EKAGLHPTSRGFAGNIVLGDIIVAVDDKPVKNKAELMKILDEYSVGDKVTLKIKRGNEDL 438

Query: 387 KIPVILEPK 395
           ++ + LE K
Sbjct: 439 ELKISLEEK 446

[Close]

BLAST of Cucsat.G10112.T12 vs. ExPASy Swiss-Prot
Match: Q2YMX6 (Probable periplasmic serine endoprotease DegP-like OS=Brucella abortus (strain 2308) OX=359391 GN=htrA PE=3 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 1.7e-43
Identity = 108/264 (40.91%), Postives = 149/264 (56.44%), Query Frame = 0


Query: 139 AVRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNP 198
           A  V L D T  DAK++G D   D+AVL I+APK K   +  G    + VG  V A+GNP
Sbjct: 159 AYTVVLDDGTELDAKLIGADPRTDLAVLKINAPKRKFVYVAFGDDNKVRVGDWVVAVGNP 218

Query: 199 FGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTA 258
           FGL  T+T+G++S   R+I +     P  D IQ DAA+N GNSGGP  D SG +IGINTA
Sbjct: 219 FGLGGTVTSGIVSARGRDIGAG----PYDDFIQIDAAVNKGNSGGPAFDLSGEVIGINTA 278

Query: 259 IYSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAP--DQSVEQLGVS---G 318
           I+SPSG S G+ F+IP  T   +VDQL++ G V R  +G++  P        LG++   G
Sbjct: 279 IFSPSGGSVGIAFAIPSSTAKQVVDQLIKKGSVERGWIGVQIQPVTKDIAASLGLAEEKG 338

Query: 319 VLVLDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDK 378
            +V     +GPA KAG+             GD+IT+VNG+ V +  DL R +     G+K
Sbjct: 339 AIVASPQDDGPAAKAGIK-----------AGDVITAVNGETVQDPRDLARKVANIAPGEK 398

Query: 379 VTVEVLRGDHMEKIPVILEPKPDE 398
             + V R +  E+I V +   P++
Sbjct: 399 AALTVWRKNKAEEINVTIAAMPND 407

[Close]

BLAST of Cucsat.G10112.T12 vs. ExPASy Swiss-Prot
Match: P0C114 (Probable periplasmic serine endoprotease DegP-like OS=Brucella abortus biovar 1 (strain 9-941) OX=262698 GN=htrA PE=3 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 1.7e-43
Identity = 108/264 (40.91%), Postives = 149/264 (56.44%), Query Frame = 0


Query: 139 AVRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNP 198
           A  V L D T  DAK++G D   D+AVL I+APK K   +  G    + VG  V A+GNP
Sbjct: 159 AYTVVLDDGTELDAKLIGADPRTDLAVLKINAPKRKFVYVAFGDDNKVRVGDWVVAVGNP 218

Query: 199 FGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTA 258
           FGL  T+T+G++S   R+I +     P  D IQ DAA+N GNSGGP  D SG +IGINTA
Sbjct: 219 FGLGGTVTSGIVSARGRDIGAG----PYDDFIQIDAAVNKGNSGGPAFDLSGEVIGINTA 278

Query: 259 IYSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAP--DQSVEQLGVS---G 318
           I+SPSG S G+ F+IP  T   +VDQL++ G V R  +G++  P        LG++   G
Sbjct: 279 IFSPSGGSVGIAFAIPSSTAKQVVDQLIKKGSVERGWIGVQIQPVTKDIAASLGLAEEKG 338

Query: 319 VLVLDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDK 378
            +V     +GPA KAG+             GD+IT+VNG+ V +  DL R +     G+K
Sbjct: 339 AIVASPQDDGPAAKAGIK-----------AGDVITAVNGETVQDPRDLARKVANIAPGEK 398

Query: 379 VTVEVLRGDHMEKIPVILEPKPDE 398
             + V R +  E+I V +   P++
Sbjct: 399 AALTVWRKNKAEEINVTIAAMPND 407

[Close]

BLAST of Cucsat.G10112.T12 vs. ExPASy Swiss-Prot
Match: Q8YG32 (Probable periplasmic serine endoprotease DegP-like OS=Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) OX=224914 GN=htrA PE=3 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 1.7e-43
Identity = 108/264 (40.91%), Postives = 149/264 (56.44%), Query Frame = 0


Query: 139 AVRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNP 198
           A  V L D T  DAK++G D   D+AVL I+APK K   +  G    + VG  V A+GNP
Sbjct: 159 AYTVVLDDGTELDAKLIGADPRTDLAVLKINAPKRKFVYVAFGDDNKVRVGDWVVAVGNP 218

Query: 199 FGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTA 258
           FGL  T+T+G++S   R+I +     P  D IQ DAA+N GNSGGP  D SG +IGINTA
Sbjct: 219 FGLGGTVTSGIVSARGRDIGAG----PYDDFIQIDAAVNKGNSGGPAFDLSGEVIGINTA 278

Query: 259 IYSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAP--DQSVEQLGVS---G 318
           I+SPSG S G+ F+IP  T   +VDQL++ G V R  +G++  P        LG++   G
Sbjct: 279 IFSPSGGSVGIAFAIPSSTAKQVVDQLIKKGSVERGWIGVQIQPVTKDIAASLGLAEEKG 338

Query: 319 VLVLDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDK 378
            +V     +GPA KAG+             GD+IT+VNG+ V +  DL R +     G+K
Sbjct: 339 AIVASPQDDGPAAKAGIK-----------AGDVITAVNGETVQDPRDLARKVANIAPGEK 398

Query: 379 VTVEVLRGDHMEKIPVILEPKPDE 398
             + V R +  E+I V +   P++
Sbjct: 399 AALTVWRKNKAEEINVTIAAMPND 407

[Close]

BLAST of Cucsat.G10112.T12 vs. NCBI nr
Match: XP_004142804.1 (protease Do-like 1, chloroplastic [Cucumis sativus] >KGN51390.1 hypothetical protein Csa_008466 [Cucumis sativus])

HSP 1 Score: 741 bits (1913), Expect = 3.37e-268
Identity = 398/439 (90.66%), Postives = 398/439 (90.66%), Query Frame = 0


Query: 1   MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNPTRHFRYPIFSLLHHNKKHSQF 60
           MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNPTRHFRYPIFSLLHHNKKHSQF
Sbjct: 1   MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNPTRHFRYPIFSLLHHNKKHSQF 60

Query: 61  SPSILPKLPVPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA 120
           SPSILPKLPVPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA
Sbjct: 61  SPSILPKLPVPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA 120

Query: 121 TVRLFQENTPSVVYITNLAVR--------------------------------------- 180
           TVRLFQENTPSVVYITNLAVR                                       
Sbjct: 121 TVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGASD 180

Query: 181 --VTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF 240
             VTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF
Sbjct: 181 LRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF 240

Query: 241 GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI 300
           GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI
Sbjct: 241 GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI 300

Query: 301 YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA 360
           YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA
Sbjct: 301 YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA 360

Query: 361 PANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL 398
           PANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL
Sbjct: 361 PANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL 420

[Close]

BLAST of Cucsat.G10112.T12 vs. NCBI nr
Match: XP_008458761.1 (PREDICTED: protease Do-like 1, chloroplastic isoform X1 [Cucumis melo] >KAA0037525.1 protease Do-like 1 [Cucumis melo var. makuwa] >TYK02000.1 protease Do-like 1 [Cucumis melo var. makuwa])

HSP 1 Score: 723 bits (1867), Expect = 3.43e-261
Identity = 390/439 (88.84%), Postives = 392/439 (89.29%), Query Frame = 0


Query: 1   MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNPTRHFRYPIFSLLHHNKKHSQF 60
           MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNP RHFRYPIFSLLH NKKHSQ 
Sbjct: 1   MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNPARHFRYPIFSLLHQNKKHSQI 60

Query: 61  SPSILPKLPVPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA 120
           SPSI  KLP+PSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA
Sbjct: 61  SPSIHLKLPIPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA 120

Query: 121 TVRLFQENTPSVVYITNLA----------------------------------------- 180
           TVRLFQENTPSVVYITNLA                                         
Sbjct: 121 TVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHVVTNYHVIRGASD 180

Query: 181 VRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF 240
           +RVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF
Sbjct: 181 LRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF 240

Query: 241 GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI 300
           GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI
Sbjct: 241 GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI 300

Query: 301 YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA 360
           YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA
Sbjct: 301 YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA 360

Query: 361 PANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL 398
           PANGPAGKAGL PTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL
Sbjct: 361 PANGPAGKAGLKPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL 420

[Close]

BLAST of Cucsat.G10112.T12 vs. NCBI nr
Match: XP_038890591.1 (protease Do-like 1, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 686 bits (1770), Expect = 2.11e-246
Identity = 371/428 (86.68%), Postives = 375/428 (87.62%), Query Frame = 0


Query: 12  SSLSRPPKRPPTSFFLSKSISFHNFSNPTRHFRYPIFSLLHHNKKHSQFSPSILPKLPVP 71
           S LSRPP R PTSFFLSKSISFHNFSNPTRHFRYPI SLL HNKK SQ S SI PKLP+ 
Sbjct: 13  SPLSRPPNRTPTSFFLSKSISFHNFSNPTRHFRYPILSLLPHNKKLSQISASIHPKLPIS 72

Query: 72  SNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELATVRLFQENTPS 131
           SNPFASI ESLL+FSTSVLLSF+LFVTDVDPAVAFVVTTPRKLQTDELATVRLFQENTPS
Sbjct: 73  SNPFASIWESLLIFSTSVLLSFSLFVTDVDPAVAFVVTTPRKLQTDELATVRLFQENTPS 132

Query: 132 VVYITNLA-----------------------------------------VRVTLADQTTF 191
           VVYITNLA                                         +RVTLADQTTF
Sbjct: 133 VVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGASDLRVTLADQTTF 192

Query: 192 DAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPFGLDHTLTTGVI 251
           DAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPFGLDHTLTTGVI
Sbjct: 193 DAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPFGLDHTLTTGVI 252

Query: 252 SGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAIYSPSGASSGVG 311
           SGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAIYSPSGASSGVG
Sbjct: 253 SGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAIYSPSGASSGVG 312

Query: 312 FSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDAPANGPAGKAGL 371
           FSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDAPANGPAGKAGL
Sbjct: 313 FSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDAPANGPAGKAGL 372

Query: 372 LPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVLRGDHMEKIPVI 398
            PTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVLRGDHMEKIPVI
Sbjct: 373 QPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVLRGDHMEKIPVI 432

[Close]

BLAST of Cucsat.G10112.T12 vs. NCBI nr
Match: XP_023515810.1 (protease Do-like 1, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 665 bits (1717), Expect = 2.67e-238
Identity = 364/441 (82.54%), Postives = 373/441 (84.58%), Query Frame = 0


Query: 4   AAAFSLLP-----SSLSRPPKRPPTSFFLSKSISFHNFSNPTRHFRYPIFSLLHHNKKHS 63
           AAAFSL       S LSRPP   PTSFFLSKSI FHNFSNP+ HFRYPIFS+L HNKK +
Sbjct: 2   AAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSCHFRYPIFSILGHNKKRN 61

Query: 64  QFSPSILPKLPVPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDE 123
           Q S +   KL  PSNPFASICESLL+F+TSV LSF+LFVTDVDPA AFVVTTPRKLQTDE
Sbjct: 62  QISATTDTKLTNPSNPFASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTDE 121

Query: 124 LATVRLFQENTPSVVYITNLA--------------------------------------- 183
           LATVRLFQENTPSVVYITNLA                                       
Sbjct: 122 LATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRGA 181

Query: 184 --VRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGN 243
             +RVTLADQTTFDAKVVGFDQDKDVAVL IDAPKDKLRPIPVGISADLLVGQKVFAIGN
Sbjct: 182 SDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIGN 241

Query: 244 PFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINT 303
           PFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINT
Sbjct: 242 PFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINT 301

Query: 304 AIYSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVL 363
           AIYSPSGASSGVGFSIPVDTVSGIVDQLVR+GKVTRPILGIKFAPDQSVEQLGVSGVLVL
Sbjct: 302 AIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLVL 361

Query: 364 DAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVE 398
           DAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKV NGSDLYRILDQCKVGDKV VE
Sbjct: 362 DAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVIVE 421

[Close]

BLAST of Cucsat.G10112.T12 vs. NCBI nr
Match: XP_022922573.1 (protease Do-like 1, chloroplastic [Cucurbita moschata] >KAG6602439.1 Protease Do-like 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 664 bits (1714), Expect = 7.64e-238
Identity = 364/441 (82.54%), Postives = 372/441 (84.35%), Query Frame = 0


Query: 4   AAAFSLLP-----SSLSRPPKRPPTSFFLSKSISFHNFSNPTRHFRYPIFSLLHHNKKHS 63
           AAAFSL       S LSRPP   PTSFFLSKSI FHNFSNP+ HFRYPIFSLL HNKK +
Sbjct: 2   AAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSYHFRYPIFSLLGHNKKRN 61

Query: 64  QFSPSILPKLPVPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDE 123
           Q S +   KL  PSNP ASICESLL+F+TSV LSF+LFVTDVDPA AFVVTTPRKLQTDE
Sbjct: 62  QISATTDTKLTNPSNPLASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTDE 121

Query: 124 LATVRLFQENTPSVVYITNLA--------------------------------------- 183
           LATVRLFQENTPSVVYITNLA                                       
Sbjct: 122 LATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRGA 181

Query: 184 --VRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGN 243
             +RVTLADQTTFDAKVVGFDQDKDVAVL IDAPKDKLRPIPVGISADLLVGQKVFAIGN
Sbjct: 182 SDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIGN 241

Query: 244 PFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINT 303
           PFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINT
Sbjct: 242 PFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINT 301

Query: 304 AIYSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVL 363
           AIYSPSGASSGVGFSIPVDTVSGIVDQLVR+GKVTRPILGIKFAPDQSVEQLGVSGVLVL
Sbjct: 302 AIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLVL 361

Query: 364 DAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVE 398
           DAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKV NGSDLYRILDQCKVGDKV VE
Sbjct: 362 DAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVIVE 421

[Close]

BLAST of Cucsat.G10112.T12 vs. ExPASy TrEMBL
Match: A0A0A0KP41 (PDZ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G524830 PE=3 SV=1)

HSP 1 Score: 741 bits (1913), Expect = 1.63e-268
Identity = 398/439 (90.66%), Postives = 398/439 (90.66%), Query Frame = 0


Query: 1   MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNPTRHFRYPIFSLLHHNKKHSQF 60
           MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNPTRHFRYPIFSLLHHNKKHSQF
Sbjct: 1   MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNPTRHFRYPIFSLLHHNKKHSQF 60

Query: 61  SPSILPKLPVPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA 120
           SPSILPKLPVPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA
Sbjct: 61  SPSILPKLPVPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA 120

Query: 121 TVRLFQENTPSVVYITNLAVR--------------------------------------- 180
           TVRLFQENTPSVVYITNLAVR                                       
Sbjct: 121 TVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGASD 180

Query: 181 --VTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF 240
             VTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF
Sbjct: 181 LRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF 240

Query: 241 GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI 300
           GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI
Sbjct: 241 GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI 300

Query: 301 YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA 360
           YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA
Sbjct: 301 YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA 360

Query: 361 PANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL 398
           PANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL
Sbjct: 361 PANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL 420

[Close]

BLAST of Cucsat.G10112.T12 vs. ExPASy TrEMBL
Match: A0A5A7T7V2 (Protease Do-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G001140 PE=3 SV=1)

HSP 1 Score: 723 bits (1867), Expect = 1.66e-261
Identity = 390/439 (88.84%), Postives = 392/439 (89.29%), Query Frame = 0


Query: 1   MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNPTRHFRYPIFSLLHHNKKHSQF 60
           MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNP RHFRYPIFSLLH NKKHSQ 
Sbjct: 1   MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNPARHFRYPIFSLLHQNKKHSQI 60

Query: 61  SPSILPKLPVPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA 120
           SPSI  KLP+PSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA
Sbjct: 61  SPSIHLKLPIPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA 120

Query: 121 TVRLFQENTPSVVYITNLA----------------------------------------- 180
           TVRLFQENTPSVVYITNLA                                         
Sbjct: 121 TVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHVVTNYHVIRGASD 180

Query: 181 VRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF 240
           +RVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF
Sbjct: 181 LRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF 240

Query: 241 GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI 300
           GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI
Sbjct: 241 GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI 300

Query: 301 YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA 360
           YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA
Sbjct: 301 YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA 360

Query: 361 PANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL 398
           PANGPAGKAGL PTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL
Sbjct: 361 PANGPAGKAGLKPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL 420

[Close]

BLAST of Cucsat.G10112.T12 vs. ExPASy TrEMBL
Match: A0A1S3C962 (protease Do-like 1, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498072 PE=3 SV=1)

HSP 1 Score: 723 bits (1867), Expect = 1.66e-261
Identity = 390/439 (88.84%), Postives = 392/439 (89.29%), Query Frame = 0


Query: 1   MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNPTRHFRYPIFSLLHHNKKHSQF 60
           MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNP RHFRYPIFSLLH NKKHSQ 
Sbjct: 1   MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNPARHFRYPIFSLLHQNKKHSQI 60

Query: 61  SPSILPKLPVPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA 120
           SPSI  KLP+PSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA
Sbjct: 61  SPSIHLKLPIPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA 120

Query: 121 TVRLFQENTPSVVYITNLA----------------------------------------- 180
           TVRLFQENTPSVVYITNLA                                         
Sbjct: 121 TVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHVVTNYHVIRGASD 180

Query: 181 VRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF 240
           +RVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF
Sbjct: 181 LRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF 240

Query: 241 GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI 300
           GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI
Sbjct: 241 GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI 300

Query: 301 YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA 360
           YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA
Sbjct: 301 YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA 360

Query: 361 PANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL 398
           PANGPAGKAGL PTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL
Sbjct: 361 PANGPAGKAGLKPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL 420

[Close]

BLAST of Cucsat.G10112.T12 vs. ExPASy TrEMBL
Match: A0A6J1JTP9 (protease Do-like 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111487418 PE=3 SV=1)

HSP 1 Score: 664 bits (1714), Expect = 3.70e-238
Identity = 364/441 (82.54%), Postives = 373/441 (84.58%), Query Frame = 0


Query: 4   AAAFSLLP-----SSLSRPPKRPPTSFFLSKSISFHNFSNPTRHFRYPIFSLLHHNKKHS 63
           AAAFSL       S LSRPP   PTSFFLSKSI FHNFSNP+ HFRYPIFSLL H KK +
Sbjct: 2   AAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSCHFRYPIFSLLGHTKKRN 61

Query: 64  QFSPSILPKLPVPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDE 123
           Q S +   KL  PSNPFASICESLL+F+TSV LSF+LFVTDVDPA AFVVTTPRKLQTDE
Sbjct: 62  QISATTDTKLTNPSNPFASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTDE 121

Query: 124 LATVRLFQENTPSVVYITNLA--------------------------------------- 183
           LATVRLFQENTPSVVYITNLA                                       
Sbjct: 122 LATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRGA 181

Query: 184 --VRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGN 243
             +RVTLADQTTFDAKVVGFDQDKDVAVL IDAPKDKLRPIPVGISADLLVGQKVFAIGN
Sbjct: 182 SDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIGN 241

Query: 244 PFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINT 303
           PFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINT
Sbjct: 242 PFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINT 301

Query: 304 AIYSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVL 363
           AIYSPSGASSGVGFSIPVDTVSGIVDQLVR+GKVTRPILGIKFAPDQSVEQLGVSGVLVL
Sbjct: 302 AIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLVL 361

Query: 364 DAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVE 398
           DAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVG+KV VE
Sbjct: 362 DAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGEKVIVE 421

[Close]

BLAST of Cucsat.G10112.T12 vs. ExPASy TrEMBL
Match: A0A6J1E723 (protease Do-like 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111430538 PE=3 SV=1)

HSP 1 Score: 664 bits (1714), Expect = 3.70e-238
Identity = 364/441 (82.54%), Postives = 372/441 (84.35%), Query Frame = 0


Query: 4   AAAFSLLP-----SSLSRPPKRPPTSFFLSKSISFHNFSNPTRHFRYPIFSLLHHNKKHS 63
           AAAFSL       S LSRPP   PTSFFLSKSI FHNFSNP+ HFRYPIFSLL HNKK +
Sbjct: 2   AAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSYHFRYPIFSLLGHNKKRN 61

Query: 64  QFSPSILPKLPVPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDE 123
           Q S +   KL  PSNP ASICESLL+F+TSV LSF+LFVTDVDPA AFVVTTPRKLQTDE
Sbjct: 62  QISATTDTKLTNPSNPLASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTDE 121

Query: 124 LATVRLFQENTPSVVYITNLA--------------------------------------- 183
           LATVRLFQENTPSVVYITNLA                                       
Sbjct: 122 LATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRGA 181

Query: 184 --VRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGN 243
             +RVTLADQTTFDAKVVGFDQDKDVAVL IDAPKDKLRPIPVGISADLLVGQKVFAIGN
Sbjct: 182 SDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIGN 241

Query: 244 PFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINT 303
           PFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINT
Sbjct: 242 PFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINT 301

Query: 304 AIYSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVL 363
           AIYSPSGASSGVGFSIPVDTVSGIVDQLVR+GKVTRPILGIKFAPDQSVEQLGVSGVLVL
Sbjct: 302 AIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLVL 361

Query: 364 DAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVE 398
           DAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKV NGSDLYRILDQCKVGDKV VE
Sbjct: 362 DAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVIVE 421

The following BLAST results are available for this feature:

BLAST of Cucsat.G10112.T12 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22609	2.5e-148	70.18	Protease Do-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP1 PE=1 ...	[more]
Q9LU10	3.1e-66	56.22	Protease Do-like 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP8 PE=1 ...	[more]
Q2YMX6	1.7e-43	40.91	Probable periplasmic serine endoprotease DegP-like OS=Brucella abortus (strain 2...	[more]
P0C114	1.7e-43	40.91	Probable periplasmic serine endoprotease DegP-like OS=Brucella abortus biovar 1 ...	[more]
Q8YG32	1.7e-43	40.91	Probable periplasmic serine endoprotease DegP-like OS=Brucella melitensis biotyp...	[more]

BLAST of Cucsat.G10112.T12 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004142804.1	3.37e-268	90.66	protease Do-like 1, chloroplastic [Cucumis sativus] >KGN51390.1 hypothetical pro...	[more]
XP_008458761.1	3.43e-261	88.84	PREDICTED: protease Do-like 1, chloroplastic isoform X1 [Cucumis melo] >KAA00375...	[more]
XP_038890591.1	2.11e-246	86.68	protease Do-like 1, chloroplastic isoform X1 [Benincasa hispida]	[more]
XP_023515810.1	2.67e-238	82.54	protease Do-like 1, chloroplastic [Cucurbita pepo subsp. pepo]	[more]
XP_022922573.1	7.64e-238	82.54	protease Do-like 1, chloroplastic [Cucurbita moschata] >KAG6602439.1 Protease Do...	[more]

BLAST of Cucsat.G10112.T12 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KP41	1.63e-268	90.66	PDZ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G524830 PE=3 SV...	[more]
A0A5A7T7V2	1.66e-261	88.84	Protease Do-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G0...	[more]
A0A1S3C962	1.66e-261	88.84	protease Do-like 1, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349...	[more]
A0A6J1JTP9	3.70e-238	82.54	protease Do-like 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111487418 PE...	[more]
A0A6J1E723	3.70e-238	82.54	protease Do-like 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111430538 ...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001940	Peptidase S1C	PRINTS	PR00834	PROTEASES2C	coord: 226..243 score: 72.49 coord: 340..352 score: 44.84 coord: 248..265 score: 52.85 coord: 146..166 score: 43.55 coord: 188..212 score: 55.41
IPR001478	PDZ domain	SMART	SM00228	pdz_new	coord: 294..382 e-value: 2.4E-9 score: 47.1
IPR001478	PDZ domain	PFAM	PF13180	PDZ_2	coord: 294..391 e-value: 1.5E-14 score: 54.2
IPR001478	PDZ domain	PROSITE	PS50106	PDZ	coord: 285..382 score: 10.9785
None	No IPR available	PFAM	PF13365	Trypsin_2	coord: 138..255 e-value: 3.3E-25 score: 89.8
None	No IPR available	GENE3D	2.40.10.120		coord: 126..291 e-value: 1.7E-55 score: 189.7
None	No IPR available	PANTHER	PTHR43019:SF38	PROTEASE DO-LIKE 1, CHLOROPLASTIC	coord: 140..398 coord: 57..139
None	No IPR available	PANTHER	PTHR43019	SERINE ENDOPROTEASE DEGS	coord: 140..398 coord: 57..139
None	No IPR available	CDD	cd00987	PDZ_serine_protease	coord: 294..389 e-value: 1.42594E-18 score: 78.0651
IPR036034	PDZ superfamily	GENE3D	2.30.42.10		coord: 294..398 e-value: 5.3E-40 score: 137.4
IPR036034	PDZ superfamily	SUPERFAMILY	50156	PDZ domain-like	coord: 246..395
IPR009003	Peptidase S1, PA clan	SUPERFAMILY	50494	Trypsin-like serine proteases	coord: 115..300

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G10112	Cucsat.G10112	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G10112.T12.E1	Cucsat.G10112.T12.E1	exon
Cucsat.G10112.T12.E2	Cucsat.G10112.T12.E2	exon
Cucsat.G10112.T12.E3	Cucsat.G10112.T12.E3	exon
Cucsat.G10112.T12.E4	Cucsat.G10112.T12.E4	exon
Cucsat.G10112.T12.E5	Cucsat.G10112.T12.E5	exon
Cucsat.G10112.T12.E6	Cucsat.G10112.T12.E6	exon
Cucsat.G10112.T12.E7	Cucsat.G10112.T12.E7	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G10112.T12.C1	Cucsat.G10112.T12.C1	CDS
Cucsat.G10112.T12.C2	Cucsat.G10112.T12.C2	CDS
Cucsat.G10112.T12.C3	Cucsat.G10112.T12.C3	CDS
Cucsat.G10112.T12.C4	Cucsat.G10112.T12.C4	CDS
Cucsat.G10112.T12.C5	Cucsat.G10112.T12.C5	CDS
Cucsat.G10112.T12.C6	Cucsat.G10112.T12.C6	CDS
Cucsat.G10112.T12.C7	Cucsat.G10112.T12.C7	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G10112.T12	Cucsat.G10112.T12-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0005515	protein binding
molecular_function	GO:0004252	serine-type endopeptidase activity