Csor.00g305350.m01 (mRNA) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g305350.m01
TypemRNA
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionPyruvate kinase
LocationCsor_Chr18: 12828119 .. 12832494 (+)
Sequence length1722
RNA-Seq ExpressionCsor.00g305350.m01
SyntenyCsor.00g305350.m01
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTCAGGTGGTGGCTACGAGGTCGATTCACAGCACAGTCCCGTGCCATGGCTCGGGATCTGTGCTGGACCGGGTGAACAGAGCTCGGTCTTTTGGTATTGGATCCAAAGTGTTGGCGCATGAGAAGAAGAAAAGGAATTTTGTTTGCAGGAGAAGTCTAATCATAGCCAAGAGAGCTGCTCAAGCTGAAGTTGTTCCGGTATCACCTGAGGATATTCAGAAGGTTTGTGATCTCCTGATTTGTTAGTTCTGTCTTTAAGGAAATTGCTTTTATTTGCTTGTTACGATAAGATTGAGGAGAAATGGGGAGCTGAAAGTCATGGAATTTGAATTTTGAAAAGGAAAATGCTTTTGTTTGAAAGGCACGTAGCATTCAGAAAGCGAAATGGTGTACTTTGTTTGTATATCGTCGAGTTGTACGGATCTGTAATTGGATGCCTAGACTTTTTGTGCGAGCTGGAAATTTAGTATGCCTGTTTCTGTTCCTGGGAAAGTGAAGTATGATCCATAAATGGGTTTTCTTTGATCTCTGAGTTTTCGGAAGGGAACATACATATGTGCATGCACACTCACAAATTTATCTTTCTATATCTGTATAAGCGGTTGCTCGGGAAGCCTCATCTCTTATTTTGTTTAAATATGACAACGGATATTTTGTAGCCTTCATTGGCTACAAATGTTTGCGAATTTTAATTTTCACGTGTTAATTTGATTCTGAATGCTGCATATCAGGCAGATGAGCAATTTGAACATTTACGAGCAGTTCAACAACTCGGTGATACACCGGTCGGAATGTGGTCCAAACCAACAGTTAGACGTAAGACAAAAATCGTGTGCACAATTGGACCTTCAACTAACACAAAGGAAATGATTTGGAAGCTGGCTGAGGCTGGAATGAACGTTGCCCGATTGAATATGTCTCATGGAGACCATGCATCTCATCAGAAAGTTATAGATTTAGTCAGAGAATACAATGCCCAATGTAAGGACAACTGCATTGCAATCATGCTCGATACCAAGGCAAGTTCATTTTCACATTTTTCCACGTTCACCACCTTCGATCGATTAAACTGGCAACAAACGTTTTCCAGATAGACCAAGGTATTTTTTATTTTATTTTTAAAATTATCTACTTCATTTTGTCAGGGTCCTGAGGTTAGGAGTGGTGACCTACCACAACCAATATTGTTAGAAAGTGGACAGGAATTCACTTTTACCATACGGAGAGGTGTTAGCACTGCAGACTCTGTTAGCGTGAACTATGATGATTTTGTTAATGATGTAGAAGTTGGGGACATCCTTCTTGTTGATGGTATGCTGTTTTTATTTTCTTTATTCCCTATTTTCTGTGTATATATTTTTTGCCACTAATTAGAAACTTTTTTAGATAGAATAACCAGGTTACATTCCTTGTGGTGAAAAGTCTTGGGTTCATAGCATAGGAGTATAGTGTTATATGAGCATGAAAATCTAGTGTGCATCAGTTCTCTCTTTCTTTACTTTCTTTGACATTATTACTTGAAATTTCTATAAAAAAAATAAAGAACCTTTCTTTCATCATCTCATGTGTTGCTACAACATTACTCTTTAAATCTCTTATAGGTGGAATGATGTCGTTCATGGTGAAGTCTAAGACAGAGGATTCTGTTAAGTGTGAAGTTATTGATGGTGGAGAGCTCAAGTCCAGGCGACATTTAAATGTTCGAGGGAAAAGTGCTACACTGCCTTCCATTACTGGTTGGGCTTTGAATTAATATATCCTACACATTCCATTTTTTATTGAAGTTTCATTTCGAGTAGTACGGGATTAATAGGGCTTTCATTCCTTTTTACTTCTTTTGCGTGTGAGTCAACTAATATGTTTTGCTGTTTATGTTTCTTGCAGAGAAGGACTGGGATGACATAAAATTTGGAGTTGACAACAAAGTTGACTTTTATGCTGTCTCGTTTGTTAAAGATGCACAAGTAGTTCATGAGTTGAAAAAATATCTTAAAAGTACATAACTGCAGATCTCTATGTTGTGCTTATAAAGTCAATTTGGGTGCTGCATTCTTGTTAGACGTTGATTTATTAACTTTTCTGACTCTGCAGGCTGTGATGCTGATATTCATGTCATTGTAAAAATAGAAAGTGCAGACTCCATACCAAATTTGCATTCAATTATTACAGCTTCTGATGGGGTAATTATTTGATTTTTTTCACAACATCGGAGTGGATATTCCAATGTTAATGTTGGATTCTAAGCATTTTTAATTTTGTTGATAGTCTGACGCTTGGTTGCTAATTTAAGGGGAAATTCCTTTATCTCATTCTAGGCAATGGTTGCAAGAGGCGATCTCGGTGCAGAACTCCCTATTGAAGAAGTTCCACTTCTGCAGGTAGGTAGTTTTTCCAATTAAAGAAGCTGAGGAGGTTTATTTTCAGTCATGCTTTTCTTCCACTCATAGAAGAGATGTTTAGTGTTGTTTATTTGTGAAACTTTCTAAACATACTTGATCGATATGAAGGAGGAGATAATCAACTTGTGTCGAGGCATGGGGAAGGCTGTCATTGTTGCAACTAACATGCTTGAGAGTATGATTGTACATCCGACACCAACCAGAGCAGAAGTATCTGATATTGCTATTGCAGTTAGAGAGGGGGCCGATGCAATCATGCTCTCTGGAGAAACAGCTCATGGAAAGTGAGTTATCTCTTTAGTTCCTTTTTATGCTATTAGTTAATCTGTGGTTGGCTCAGACATTTTGGGGCACAGTACATGGTTTTTGCAATGTTTGGGATTTTTCCGGATTATCTTGTTTCTTTAGCTCGTGCGATGCTGCAGATATCGAGCATATTATATACAGTTCATATAACAAAAAAAAAAAAAAAAGGACTAAACCAATTTTGAGAAAATAAACTCACTTCAGCTGGCTACTTATATTGTCTTGGTTTCTATTTGGTGAATAAAATAAACAAAGTATATTCGTTCCTTTGATCACCTCATTATGCCAATTTCCAGATTCCCCTTAAAAGCTGTGAAAGTAATGCATACAGTTGCTTTACGGACTGAAGCAACCATAGAAGGTGGTCGAATGCCATTGAATCTCGGTCAAACATTCAAGGTTATTTTAAAACTAGTAAATTTATGGTACTTCAATAAGACCATGATTTGTTGCCTCCCGCTCAGGCTCCTGTTCTTTTTTATTAGTTAATCTTTATCATGATTCTGACTTGTTTACTTTTCTTCTATGTAGAACCATATGAGTGAGATGTTTGCTTATCATGCAACAATGATGTCCAACACTCTTGGAACCTCAATAGTTGTCTTCACTAAAACTGGTTTCATGGCCATCCTTCTAAGCCATTACCGACCATCTGGGACAACATTTGCTTTTACAAATGAGTGAGTAGCCCGTGTATTTTCTTTTACGAAAGAGTTGATTGTTACTAGAAAGCCAATCATACACTACGTGTCCTAGAAATGTTTGCATTCAACATTCCTACTTTTTCCTTTTTGTTATTATTGTTTTCATAGTCTGATAGAATCTAAACTTGTGTTTATCTAACTGCTTCATCACACTTTTGTTATCATTTCTGTGTCAAGATTACATGATTTTGATCGTTCCTTTTATTATACTCAAAGTACGATCATAAGGGCTATGTGGGGTTGATTGACTAAACATCAAATACAAACAGTCTAGTTCATTGTTGATGAAAATGTTTGCAATTTCAATATTGATCCACTGAAGTTGTTAAACCACCCCTAAGATCCATAAAAGGAGCCAGTATCATGGATGAAAAGCTTTCCTGTATGCTATGGATTGAGTTATCCATGAATAAGAAATATACAATTTAGTTAGGTACTAGGACTTCAGCTATGATAGTTTCCTTAAATACCCTACTGTGACGTGCAGTAAAAGGATACAACAGAGGTTGGCTTTATATCAGGGAGTGTGCCCCATATACATGCAGTTCTCTGATGATGCTGAGCAGACCTTTGCTGATGCCTTATCTCTGCTACAGGTATCGTATCCGCCAATCCGTTTTATTTTTCCTTTTTCTTCTTTTTTTCAATAACATTTTCTATGGTTAATAGTTAATAAGGACCACGTAAATAATAAATGGCCCAAGGGAATGAGTAGTAGGGTCAGACAATTGTCCCATAAGAATCGTTGCTAGCGTGAATGAATGTGAAAAACAAAAACAAAAAAGCTTCTGAATGTAATCTCTAGTTTCCTTGTATGAGTATCTGTTTGACTCATTATTGAGTTGGTTGTGTACAGAGGCAAGGAATGGTAAAGGAAGGAGAAGAGGTGGCACTCCTTCAGAGTGGCAGACAACCCATATGGCGGTTCCCATCTACTCACAACATTCAAGTCCGTAAAGTGTAG

mRNA sequence

ATGTCTCAGGTGGTGGCTACGAGGTCGATTCACAGCACAGTCCCGTGCCATGGCTCGGGATCTGTGCTGGACCGGGTGAACAGAGCTCGGTCTTTTGGTATTGGATCCAAAGTGTTGGCGCATGAGAAGAAGAAAAGGAATTTTGTTTGCAGGAGAAGTCTAATCATAGCCAAGAGAGCTGCTCAAGCTGAAGTTGTTCCGGTATCACCTGAGGATATTCAGAAGGCAGATGAGCAATTTGAACATTTACGAGCAGTTCAACAACTCGGTGATACACCGGTCGGAATGTGGTCCAAACCAACAGTTAGACGTAAGACAAAAATCGTGTGCACAATTGGACCTTCAACTAACACAAAGGAAATGATTTGGAAGCTGGCTGAGGCTGGAATGAACGTTGCCCGATTGAATATGTCTCATGGAGACCATGCATCTCATCAGAAAGTTATAGATTTAGTCAGAGAATACAATGCCCAATGTAAGGACAACTGCATTGCAATCATGCTCGATACCAAGGGTCCTGAGGTTAGGAGTGGTGACCTACCACAACCAATATTGTTAGAAAGTGGACAGGAATTCACTTTTACCATACGGAGAGGTGTTAGCACTGCAGACTCTGTTAGCGTGAACTATGATGATTTTGTTAATGATGTAGAAGTTGGGGACATCCTTCTTGTTGATGGTGGAATGATGTCGTTCATGGTGAAGTCTAAGACAGAGGATTCTGTTAAGTGTGAAGTTATTGATGGTGGAGAGCTCAAGTCCAGGCGACATTTAAATGTTCGAGGGAAAAGTGCTACACTGCCTTCCATTACTGAGAAGGACTGGGATGACATAAAATTTGGAGTTGACAACAAAGTTGACTTTTATGCTGTCTCGTTTGTTAAAGATGCACAAGTAGTTCATGAGTTGAAAAAATATCTTAAAAGCTGTGATGCTGATATTCATGTCATTGTAAAAATAGAAAGTGCAGACTCCATACCAAATTTGCATTCAATTATTACAGCTTCTGATGGGGCAATGGTTGCAAGAGGCGATCTCGGTGCAGAACTCCCTATTGAAGAAGTTCCACTTCTGCAGGAGGAGATAATCAACTTGTGTCGAGGCATGGGGAAGGCTGTCATTGTTGCAACTAACATGCTTGAGAGTATGATTGTACATCCGACACCAACCAGAGCAGAAGTATCTGATATTGCTATTGCAGTTAGAGAGGGGGCCGATGCAATCATGCTCTCTGGAGAAACAGCTCATGGAAAATTCCCCTTAAAAGCTGTGAAAGTAATGCATACAGTTGCTTTACGGACTGAAGCAACCATAGAAGGTGGTCGAATGCCATTGAATCTCGGTCAAACATTCAAGAACCATATGAGTGAGATGTTTGCTTATCATGCAACAATGATGTCCAACACTCTTGGAACCTCAATAGTTGTCTTCACTAAAACTGGTTTCATGGCCATCCTTCTAAGCCATTACCGACCATCTGGGACAACATTTGCTTTTACAAATGATAAAAGGATACAACAGAGGTTGGCTTTATATCAGGGAGTGTGCCCCATATACATGCAGTTCTCTGATGATGCTGAGCAGACCTTTGCTGATGCCTTATCTCTGCTACAGAGGCAAGGAATGGTAAAGGAAGGAGAAGAGGTGGCACTCCTTCAGAGTGGCAGACAACCCATATGGCGGTTCCCATCTACTCACAACATTCAAGTCCGTAAAGTGTAG

Coding sequence (CDS)

ATGTCTCAGGTGGTGGCTACGAGGTCGATTCACAGCACAGTCCCGTGCCATGGCTCGGGATCTGTGCTGGACCGGGTGAACAGAGCTCGGTCTTTTGGTATTGGATCCAAAGTGTTGGCGCATGAGAAGAAGAAAAGGAATTTTGTTTGCAGGAGAAGTCTAATCATAGCCAAGAGAGCTGCTCAAGCTGAAGTTGTTCCGGTATCACCTGAGGATATTCAGAAGGCAGATGAGCAATTTGAACATTTACGAGCAGTTCAACAACTCGGTGATACACCGGTCGGAATGTGGTCCAAACCAACAGTTAGACGTAAGACAAAAATCGTGTGCACAATTGGACCTTCAACTAACACAAAGGAAATGATTTGGAAGCTGGCTGAGGCTGGAATGAACGTTGCCCGATTGAATATGTCTCATGGAGACCATGCATCTCATCAGAAAGTTATAGATTTAGTCAGAGAATACAATGCCCAATGTAAGGACAACTGCATTGCAATCATGCTCGATACCAAGGGTCCTGAGGTTAGGAGTGGTGACCTACCACAACCAATATTGTTAGAAAGTGGACAGGAATTCACTTTTACCATACGGAGAGGTGTTAGCACTGCAGACTCTGTTAGCGTGAACTATGATGATTTTGTTAATGATGTAGAAGTTGGGGACATCCTTCTTGTTGATGGTGGAATGATGTCGTTCATGGTGAAGTCTAAGACAGAGGATTCTGTTAAGTGTGAAGTTATTGATGGTGGAGAGCTCAAGTCCAGGCGACATTTAAATGTTCGAGGGAAAAGTGCTACACTGCCTTCCATTACTGAGAAGGACTGGGATGACATAAAATTTGGAGTTGACAACAAAGTTGACTTTTATGCTGTCTCGTTTGTTAAAGATGCACAAGTAGTTCATGAGTTGAAAAAATATCTTAAAAGCTGTGATGCTGATATTCATGTCATTGTAAAAATAGAAAGTGCAGACTCCATACCAAATTTGCATTCAATTATTACAGCTTCTGATGGGGCAATGGTTGCAAGAGGCGATCTCGGTGCAGAACTCCCTATTGAAGAAGTTCCACTTCTGCAGGAGGAGATAATCAACTTGTGTCGAGGCATGGGGAAGGCTGTCATTGTTGCAACTAACATGCTTGAGAGTATGATTGTACATCCGACACCAACCAGAGCAGAAGTATCTGATATTGCTATTGCAGTTAGAGAGGGGGCCGATGCAATCATGCTCTCTGGAGAAACAGCTCATGGAAAATTCCCCTTAAAAGCTGTGAAAGTAATGCATACAGTTGCTTTACGGACTGAAGCAACCATAGAAGGTGGTCGAATGCCATTGAATCTCGGTCAAACATTCAAGAACCATATGAGTGAGATGTTTGCTTATCATGCAACAATGATGTCCAACACTCTTGGAACCTCAATAGTTGTCTTCACTAAAACTGGTTTCATGGCCATCCTTCTAAGCCATTACCGACCATCTGGGACAACATTTGCTTTTACAAATGATAAAAGGATACAACAGAGGTTGGCTTTATATCAGGGAGTGTGCCCCATATACATGCAGTTCTCTGATGATGCTGAGCAGACCTTTGCTGATGCCTTATCTCTGCTACAGAGGCAAGGAATGGTAAAGGAAGGAGAAGAGGTGGCACTCCTTCAGAGTGGCAGACAACCCATATGGCGGTTCCCATCTACTCACAACATTCAAGTCCGTAAAGTGTAG

Protein sequence

MSQVVATRSIHSTVPCHGSGSVLDRVNRARSFGIGSKVLAHEKKKRNFVCRRSLIIAKRAAQAEVVPVSPEDIQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQCKDNCIAIMLDTKGPEVRSGDLPQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDILLVDGGMMSFMVKSKTEDSVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVVHELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQEEIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFPLKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTKTGFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Homology
BLAST of Csor.00g305350.m01 vs. ExPASy Swiss-Prot
Match: Q9FLW9 (Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1)

HSP 1 Score: 912.1 bits (2356), Expect = 3.1e-264
Identity = 463/579 (79.97%), Postives = 506/579 (87.39%), Query Frame = 0

Query: 1   MSQVVATRSIHSTVPCHGSGSVLDRVNR-ARSFGIGSKVLAHEKKKRNFVCRRS-----L 60
           M+QVVATRSI  ++     GSV  R  +  +      KVL +E K+   V  RS      
Sbjct: 1   MAQVVATRSIQGSMLSPNGGSVSTRSEKLLKPASFAVKVLGNEAKRSGRVSVRSRRVVDT 60

Query: 61  IIAKRAAQAEVVPVSPEDIQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGP 120
            +     + EV+PVSPED+   +EQ E L  +QQ GDT VGMWSKPTVRRKTKIVCT+GP
Sbjct: 61  TVRSARVETEVIPVSPEDVPNREEQLERLLEMQQFGDTSVGMWSKPTVRRKTKIVCTVGP 120

Query: 121 STNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQCKDNCIAIMLDTKGPE 180
           STNT+EMIWKLAEAGMNVAR+NMSHGDHASH+KVIDLV+EYNAQ KDN IAIMLDTKGPE
Sbjct: 121 STNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNTIAIMLDTKGPE 180

Query: 181 VRSGDLPQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDILLVDGGMMSFMV 240
           VRSGDLPQPI+L+ GQEFTFTI RGVST   VSVNYDDFVNDVE GD+LLVDGGMMSFMV
Sbjct: 181 VRSGDLPQPIMLDPGQEFTFTIERGVSTPSCVSVNYDDFVNDVEAGDMLLVDGGMMSFMV 240

Query: 241 KSKTEDSVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFV 300
           KSKT+DSVKCEV+DGGELKSRRHLNVRGKSATLPSITEKDW+DIKFGV+NKVDFYAVSFV
Sbjct: 241 KSKTKDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWEDIKFGVENKVDFYAVSFV 300

Query: 301 KDAQVVHELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEE 360
           KDAQVVHELKKYL++  ADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEE
Sbjct: 301 KDAQVVHELKKYLQNSGADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEE 360

Query: 361 VPLLQEEIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGET 420
           VP+LQEEIINLCR MGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADA+MLSGET
Sbjct: 361 VPILQEEIINLCRSMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGET 420

Query: 421 AHGKFPLKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSI 480
           AHGKFPLKA  VMHTVALRTEATI  G MP NLGQ FKNHMSEMFAYHATMMSNTLGTS 
Sbjct: 421 AHGKFPLKAAGVMHTVALRTEATITSGEMPPNLGQAFKNHMSEMFAYHATMMSNTLGTST 480

Query: 481 VVFTKTGFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFADAL 540
           VVFT+TGFMAILLSHYRPSGT +AFTN+K+IQQRLALYQGVCPIYM+F+DDAE+TFA+AL
Sbjct: 481 VVFTRTGFMAILLSHYRPSGTIYAFTNEKKIQQRLALYQGVCPIYMEFTDDAEETFANAL 540

Query: 541 SLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           + L +QGMVK+GEE+A++QSG QPIWR  STHNIQVRKV
Sbjct: 541 ATLLKQGMVKKGEEIAIVQSGTQPIWRSQSTHNIQVRKV 579

BLAST of Csor.00g305350.m01 vs. ExPASy Swiss-Prot
Match: Q40546 (Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 738.8 bits (1906), Expect = 4.6e-212
Identity = 367/470 (78.09%), Postives = 418/470 (88.94%), Query Frame = 0

Query: 104 RKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQCKDNC 163
           RKTKIVCTIGPST+++EMIWKLAEAGMNVARLNMSHGDHASHQ+ IDLV+EYNAQ +D  
Sbjct: 91  RKTKIVCTIGPSTSSREMIWKLAEAGMNVARLNMSHGDHASHQRTIDLVKEYNAQFEDKV 150

Query: 164 IAIMLDTKGPEVRSGDLPQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDIL 223
           IAIMLDTKGPEV SGD+P+PILL+ GQEF F+I+RGVST D+VSVNYDDF+NDVE GDIL
Sbjct: 151 IAIMLDTKGPEVISGDVPKPILLKEGQEFNFSIKRGVSTEDTVSVNYDDFINDVEAGDIL 210

Query: 224 LVDGGMMSFMVKSKTEDSVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVD 283
           LVDGGMMS  VKSKT D VKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGV+
Sbjct: 211 LVDGGMMSLAVKSKTSDIVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVN 270

Query: 284 NKVDFYAVSFVKDAQVVHELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVAR 343
           N+VDFYAVSFVKDA+VVHELK YLKSC+ADIHVIVKIESADSIPNLHSII+ASDGAMVAR
Sbjct: 271 NQVDFYAVSFVKDAKVVHELKDYLKSCNADIHVIVKIESADSIPNLHSIISASDGAMVAR 330

Query: 344 GDLGAELPIEEVPLLQEEIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVRE 403
           GDLGAELPIEEVPLLQE+II  C+ M K VIVATNMLESMI HPTPTRAEVSDI+IAVRE
Sbjct: 331 GDLGAELPIEEVPLLQEDIIRRCQSMQKPVIVATNMLESMIDHPTPTRAEVSDISIAVRE 390

Query: 404 GADAIMLSGETAHGKFPLKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHA 463
           GADA+MLSGETAHGK+PLKAVKVMH VALRTE++++      +    +K+HM EMFA+H+
Sbjct: 391 GADAVMLSGETAHGKYPLKAVKVMHIVALRTESSLQKSTSSPSQSAAYKSHMGEMFAFHS 450

Query: 464 TMMSNTLGTSIVVFTKTGFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFS 523
           + M+NTL T I+VFT+TG MAI+LSH RPS T FAFTN++R++QRLALY GV PIYM+FS
Sbjct: 451 SSMANTLSTPIIVFTRTGSMAIILSHNRPSSTVFAFTNNERVKQRLALYHGVVPIYMEFS 510

Query: 524 DDAEQTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
            DAE+TF+ A+ LL  + +VK+G+ V L+QSG QPIWR  STH+IQVRKV
Sbjct: 511 SDAEETFSRAIKLLLSKSLVKDGQYVTLVQSGAQPIWRRHSTHHIQVRKV 560

BLAST of Csor.00g305350.m01 vs. ExPASy Swiss-Prot
Match: Q93Z53 (Plastidial pyruvate kinase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PKP3 PE=1 SV=1)

HSP 1 Score: 729.2 bits (1881), Expect = 3.7e-209
Identity = 370/471 (78.56%), Postives = 410/471 (87.05%), Query Frame = 0

Query: 103 RRKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQCKDN 162
           RRKTKIVCTIGPS++++EMIWKLAEAGMNVARLNMSHGDHASHQ  IDLV+EYN+   D 
Sbjct: 98  RRKTKIVCTIGPSSSSREMIWKLAEAGMNVARLNMSHGDHASHQITIDLVKEYNSLFVDK 157

Query: 163 CIAIMLDTKGPEVRSGDLPQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDI 222
            IAIMLDTKGPEVRSGD+PQPI LE GQEF FTI+RGVS  D+VSVNYDDFVNDVEVGDI
Sbjct: 158 AIAIMLDTKGPEVRSGDVPQPIFLEEGQEFNFTIKRGVSLKDTVSVNYDDFVNDVEVGDI 217

Query: 223 LLVDGGMMSFMVKSKTEDSVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGV 282
           LLVDGGMMS  VKSKT D VKC VIDGGEL+SRRHLNVRGKSATLPSIT+KDW+DIKFGV
Sbjct: 218 LLVDGGMMSLAVKSKTSDLVKCVVIDGGELQSRRHLNVRGKSATLPSITDKDWEDIKFGV 277

Query: 283 DNKVDFYAVSFVKDAQVVHELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVA 342
           DN+VDFYAVSFVKDA+VVHELK YLK+C ADI VIVKIESADSI NL SII+A DGAMVA
Sbjct: 278 DNQVDFYAVSFVKDAKVVHELKNYLKTCSADISVIVKIESADSIKNLPSIISACDGAMVA 337

Query: 343 RGDLGAELPIEEVPLLQEEIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVR 402
           RGDLGAELPIEEVPLLQEEII  CR + K VIVATNMLESMI HPTPTRAEVSDIAIAVR
Sbjct: 338 RGDLGAELPIEEVPLLQEEIIRRCRSIHKPVIVATNMLESMINHPTPTRAEVSDIAIAVR 397

Query: 403 EGADAIMLSGETAHGKFPLKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYH 462
           EGADAIMLSGETAHGKFPLKAV VMHTVALRTEA++   R   +    +K HM +MFA+H
Sbjct: 398 EGADAIMLSGETAHGKFPLKAVNVMHTVALRTEASLP-VRTSASRTTAYKGHMGQMFAFH 457

Query: 463 ATMMSNTLGTSIVVFTKTGFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQF 522
           A++M+NTL + ++VFT+TG MA+LLSHYRPS T FAFTN +RI QRLALYQGV PIYM+F
Sbjct: 458 ASIMANTLSSPLIVFTRTGSMAVLLSHYRPSATIFAFTNQRRIMQRLALYQGVMPIYMEF 517

Query: 523 SDDAEQTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           SDDAE T+A +L LLQ + M+KEG+ V L+QSG QPIWR  STH IQVRK+
Sbjct: 518 SDDAEDTYARSLKLLQDENMLKEGQHVTLVQSGSQPIWREESTHLIQVRKI 567

BLAST of Csor.00g305350.m01 vs. ExPASy Swiss-Prot
Match: P55964 (Pyruvate kinase isozyme G, chloroplastic (Fragment) OS=Ricinus communis OX=3988 PE=2 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 3.2e-181
Identity = 324/418 (77.51%), Postives = 364/418 (87.08%), Query Frame = 0

Query: 156 NAQCKDNCIAIMLDTKGPEVRSGDLPQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVN 215
           NAQ  DN ++IMLDTKGPEVRSGD+PQP+L E GQEF  TIRRGVST D+VSVNYDDFVN
Sbjct: 1   NAQSHDNVVSIMLDTKGPEVRSGDVPQPMLKE-GQEFNPTIRRGVSTQDTVSVNYDDFVN 60

Query: 216 DVEVGDILLVDGGMMSFMVKSKTEDSVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDW 275
           DV VGDILLVDGGMMS  VKSKT D VKC V+DGGELKSRRHLNVRGKSA LPSIT+KDW
Sbjct: 61  DVVVGDILLVDGGMMSLAVKSKTSDLVKCVVVDGGELKSRRHLNVRGKSARLPSITDKDW 120

Query: 276 DDIKFGVDNKVDFYAVSFVKDAQVVHELKKYLKSCDADIHVIVKIESADSIPNLHSIITA 335
            DIKFGVDN+VDFYAVSFVKDA+VVHELK+YLK C+ADIHVIVKIESADSIPNLHSII+A
Sbjct: 121 GDIKFGVDNQVDFYAVSFVKDAKVVHELKEYLKRCNADIHVIVKIESADSIPNLHSIISA 180

Query: 336 SDGAMVARGDLGAELPIEEVPLLQEEIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVS 395
           SDGAMVARGDLGAELPIEEVPLLQE+II  C  M K VIVATNMLESMI HPTPTRAEVS
Sbjct: 181 SDGAMVARGDLGAELPIEEVPLLQEDIIRRCHSMQKPVIVATNMLESMINHPTPTRAEVS 240

Query: 396 DIAIAVREGADAIMLSGETAHGKFPLKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHM 455
           DIAIAVREGADA+MLSGETAHGK+PLKAV+VMHTVALRTE++      P   G  +K HM
Sbjct: 241 DIAIAVREGADAVMLSGETAHGKYPLKAVRVMHTVALRTESSSPVNTTPPAQG-AYKGHM 300

Query: 456 SEMFAYHATMMSNTLGTSIVVFTKTGFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGV 515
            EMFA+HAT+M+NTL T I+VFT+TG MA+LLSHY+P+ T FAFTN++RI+QRL+LY+GV
Sbjct: 301 GEMFAFHATIMANTLNTPIIVFTRTGSMAVLLSHYQPASTIFAFTNEERIKQRLSLYRGV 360

Query: 516 CPIYMQFSDDAEQTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
            PIYM+FS DAE+TF+ AL LL  +G++ EGE V L+QSG QPIWR  STH+IQVRKV
Sbjct: 361 MPIYMEFSSDAEETFSRALQLLLNKGLLVEGEHVTLVQSGAQPIWRQESTHHIQVRKV 416

BLAST of Csor.00g305350.m01 vs. ExPASy Swiss-Prot
Match: Q43117 (Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 1.9e-104
Identity = 222/476 (46.64%), Postives = 301/476 (63.24%), Query Frame = 0

Query: 104 RKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQCKDNC 163
           R+TK+VCTIGP+T   E +  LA  GMNVAR+NM HG    H+ VI+ VR  N + K   
Sbjct: 104 RRTKLVCTIGPATCGFEELEALAVGGMNVARINMCHGTREWHKSVIERVRRLNEE-KGFA 163

Query: 164 IAIMLDTKGPEVRSGDL--PQPILLESGQEFTFTIRRGVSTAD--SVSVNYDDFVNDVEV 223
           +AIM+DT+G E+  GDL        E G+ +TF++R   S     +++VNYD F  DV+V
Sbjct: 164 VAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAYDSPRPERTINVNYDGFAEDVKV 223

Query: 224 GDILLVDGGMMSFMVKSKTEDSVKCEVIDGGELKSRRHLN-------VRGKSATLPSITE 283
           GD LLVDGGM+ F V  K    VKC   D G L  R +L        VR ++A LP+I+ 
Sbjct: 224 GDELLVDGGMVRFEVIEKIGPDVKCRCTDPGLLLPRANLTFWRDGSLVRERNAMLPTISS 283

Query: 284 KDWDDIKFGVDNKVDFYAVSFVKDAQVVHELKKYL--KSCDADIHVIVKIESADSIPNLH 343
           KDW DI FG+   VDF A+SFVK A+V++ LK Y+  +S D+DI VI KIES DS+ NL 
Sbjct: 284 KDWLDIDFGIAEGVDFIAISFVKSAEVINHLKSYIAARSRDSDIAVIAKIESIDSLKNLE 343

Query: 344 SIITASDGAMVARGDLGAELPIEEVPLLQEEIINLCRGMGKAVIVATNMLESMIVHPTPT 403
            II ASDGAMVARGDLGA++P+E+VP  Q+ I+ +CR + K VIVA+ +LESMI +PTPT
Sbjct: 344 EIIRASDGAMVARGDLGAQIPLEQVPSAQQNIVQVCRQLNKPVIVASQLLESMIEYPTPT 403

Query: 404 RAEVSDIAIAVREGADAIMLSGETAHGKFPLKAVKVMHTVAL------RTEATIEGGRMP 463
           RAEV+D++ AVR+ ADA+MLSGE+A G++P KA+ V+ +V++      R E   E   +P
Sbjct: 404 RAEVADVSEAVRQRADALMLSGESAMGQYPEKALAVLRSVSVRIEKWWREEKHHEAMELP 463

Query: 464 LNLGQTFKNHMSEMFAYHATMMSNTLGT-SIVVFTKTGFMAILLSHYRPSGTTFAFTNDK 523
             +G T+ + +SE     A  M+N LG  ++ V+TK G MA LLS  RP    FAFT   
Sbjct: 464 A-IGSTYSDSISEEICNSAAKMANNLGVDALFVYTKDGHMASLLSRCRPDCPIFAFTTTT 523

Query: 524 RIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQGMVKEGEEVALLQSGRQPI 560
            +++RL L  G+ P  + F+DD E       SLL+ +GM+K G+ V  +    Q I
Sbjct: 524 SVRRRLNLQWGLIPFRLSFADDMESNLNKTFSLLKARGMIKSGDLVIAVSDMLQSI 577

BLAST of Csor.00g305350.m01 vs. NCBI nr
Match: KAG6574217.1 (Plastidial pyruvate kinase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1128 bits (2917), Expect = 0.0
Identity = 573/573 (100.00%), Postives = 573/573 (100.00%), Query Frame = 0

Query: 1   MSQVVATRSIHSTVPCHGSGSVLDRVNRARSFGIGSKVLAHEKKKRNFVCRRSLIIAKRA 60
           MSQVVATRSIHSTVPCHGSGSVLDRVNRARSFGIGSKVLAHEKKKRNFVCRRSLIIAKRA
Sbjct: 1   MSQVVATRSIHSTVPCHGSGSVLDRVNRARSFGIGSKVLAHEKKKRNFVCRRSLIIAKRA 60

Query: 61  AQAEVVPVSPEDIQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
           AQAEVVPVSPEDIQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE
Sbjct: 61  AQAEVVPVSPEDIQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQCKDNCIAIMLDTKGPEVRSGDL 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQCKDNCIAIMLDTKGPEVRSGDL
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQCKDNCIAIMLDTKGPEVRSGDL 180

Query: 181 PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDILLVDGGMMSFMVKSKTED 240
           PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDILLVDGGMMSFMVKSKTED
Sbjct: 181 PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDILLVDGGMMSFMVKSKTED 240

Query: 241 SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300
           SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV
Sbjct: 241 SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300

Query: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360
           HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE
Sbjct: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360

Query: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420
           EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP
Sbjct: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420

Query: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTKT 480
           LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTKT
Sbjct: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTKT 480

Query: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ 540
           GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ
Sbjct: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ 540

Query: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573
           GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Sbjct: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573

BLAST of Csor.00g305350.m01 vs. NCBI nr
Match: KAG7013286.1 (Plastidial pyruvate kinase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1126 bits (2912), Expect = 0.0
Identity = 572/573 (99.83%), Postives = 572/573 (99.83%), Query Frame = 0

Query: 1   MSQVVATRSIHSTVPCHGSGSVLDRVNRARSFGIGSKVLAHEKKKRNFVCRRSLIIAKRA 60
           MSQVVATRSIHSTVPCHGSGSVLDRVNRARSFGIGSKVLAHEKKKRNFVCRRSLI AKRA
Sbjct: 1   MSQVVATRSIHSTVPCHGSGSVLDRVNRARSFGIGSKVLAHEKKKRNFVCRRSLITAKRA 60

Query: 61  AQAEVVPVSPEDIQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
           AQAEVVPVSPEDIQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE
Sbjct: 61  AQAEVVPVSPEDIQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQCKDNCIAIMLDTKGPEVRSGDL 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQCKDNCIAIMLDTKGPEVRSGDL
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQCKDNCIAIMLDTKGPEVRSGDL 180

Query: 181 PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDILLVDGGMMSFMVKSKTED 240
           PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDILLVDGGMMSFMVKSKTED
Sbjct: 181 PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDILLVDGGMMSFMVKSKTED 240

Query: 241 SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300
           SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV
Sbjct: 241 SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300

Query: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360
           HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE
Sbjct: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360

Query: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420
           EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP
Sbjct: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420

Query: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTKT 480
           LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTKT
Sbjct: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTKT 480

Query: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ 540
           GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ
Sbjct: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ 540

Query: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573
           GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Sbjct: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573

BLAST of Csor.00g305350.m01 vs. NCBI nr
Match: XP_022945683.1 (plastidial pyruvate kinase 2-like [Cucurbita moschata])

HSP 1 Score: 1124 bits (2908), Expect = 0.0
Identity = 571/573 (99.65%), Postives = 571/573 (99.65%), Query Frame = 0

Query: 1   MSQVVATRSIHSTVPCHGSGSVLDRVNRARSFGIGSKVLAHEKKKRNFVCRRSLIIAKRA 60
           MSQVVATRSIHSTVPCHGSGSVLDRVNRARSFGIGSKVLAHEKKKRNFVCRRSLI AKRA
Sbjct: 1   MSQVVATRSIHSTVPCHGSGSVLDRVNRARSFGIGSKVLAHEKKKRNFVCRRSLITAKRA 60

Query: 61  AQAEVVPVSPEDIQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
           AQ EVVPVSPEDIQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE
Sbjct: 61  AQVEVVPVSPEDIQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQCKDNCIAIMLDTKGPEVRSGDL 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQCKDNCIAIMLDTKGPEVRSGDL
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQCKDNCIAIMLDTKGPEVRSGDL 180

Query: 181 PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDILLVDGGMMSFMVKSKTED 240
           PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDILLVDGGMMSFMVKSKTED
Sbjct: 181 PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDILLVDGGMMSFMVKSKTED 240

Query: 241 SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300
           SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV
Sbjct: 241 SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300

Query: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360
           HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE
Sbjct: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360

Query: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420
           EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP
Sbjct: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420

Query: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTKT 480
           LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTKT
Sbjct: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTKT 480

Query: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ 540
           GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ
Sbjct: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ 540

Query: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573
           GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Sbjct: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573

BLAST of Csor.00g305350.m01 vs. NCBI nr
Match: XP_022968597.1 (plastidial pyruvate kinase 2-like [Cucurbita maxima])

HSP 1 Score: 1123 bits (2904), Expect = 0.0
Identity = 570/573 (99.48%), Postives = 571/573 (99.65%), Query Frame = 0

Query: 1   MSQVVATRSIHSTVPCHGSGSVLDRVNRARSFGIGSKVLAHEKKKRNFVCRRSLIIAKRA 60
           MSQVVATRSIHSTVPCHGSGSVLDRVNRARSFGIGSKVLAHEKKKRNFVCRRSLI AKRA
Sbjct: 1   MSQVVATRSIHSTVPCHGSGSVLDRVNRARSFGIGSKVLAHEKKKRNFVCRRSLITAKRA 60

Query: 61  AQAEVVPVSPEDIQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
           AQAEVVPVSPEDIQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE
Sbjct: 61  AQAEVVPVSPEDIQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQCKDNCIAIMLDTKGPEVRSGDL 180
           MIWKLAEAGMNVARLNMSHGDHASH KVIDLVREYNAQCKDNCIAIMLDTKGPEVRSGDL
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHHKVIDLVREYNAQCKDNCIAIMLDTKGPEVRSGDL 180

Query: 181 PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDILLVDGGMMSFMVKSKTED 240
           PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGD+LLVDGGMMSFMVKSKTED
Sbjct: 181 PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDMLLVDGGMMSFMVKSKTED 240

Query: 241 SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300
           SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV
Sbjct: 241 SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300

Query: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360
           HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE
Sbjct: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360

Query: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420
           EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP
Sbjct: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420

Query: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTKT 480
           LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTKT
Sbjct: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTKT 480

Query: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ 540
           GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ
Sbjct: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ 540

Query: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573
           GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Sbjct: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573

BLAST of Csor.00g305350.m01 vs. NCBI nr
Match: XP_023541338.1 (plastidial pyruvate kinase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1121 bits (2899), Expect = 0.0
Identity = 568/573 (99.13%), Postives = 571/573 (99.65%), Query Frame = 0

Query: 1   MSQVVATRSIHSTVPCHGSGSVLDRVNRARSFGIGSKVLAHEKKKRNFVCRRSLIIAKRA 60
           MSQVVATRSIHSTVPCHGSGSVLDRVNRARSFGIGSKVL HEKK+RNFVCRR+LI AKRA
Sbjct: 1   MSQVVATRSIHSTVPCHGSGSVLDRVNRARSFGIGSKVLTHEKKRRNFVCRRTLITAKRA 60

Query: 61  AQAEVVPVSPEDIQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
           AQAEVVPVSPEDIQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE
Sbjct: 61  AQAEVVPVSPEDIQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQCKDNCIAIMLDTKGPEVRSGDL 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQCKDNCIAIMLDTKGPEVRSGDL
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQCKDNCIAIMLDTKGPEVRSGDL 180

Query: 181 PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDILLVDGGMMSFMVKSKTED 240
           PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGD+LLVDGGMMSFMVKSKTED
Sbjct: 181 PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDMLLVDGGMMSFMVKSKTED 240

Query: 241 SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300
           SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV
Sbjct: 241 SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300

Query: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360
           HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE
Sbjct: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360

Query: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420
           EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP
Sbjct: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420

Query: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTKT 480
           LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTKT
Sbjct: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTKT 480

Query: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ 540
           GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ
Sbjct: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ 540

Query: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573
           GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Sbjct: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573

BLAST of Csor.00g305350.m01 vs. ExPASy TrEMBL
Match: A0A6J1G1L0 (Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111449841 PE=3 SV=1)

HSP 1 Score: 1124 bits (2908), Expect = 0.0
Identity = 571/573 (99.65%), Postives = 571/573 (99.65%), Query Frame = 0

Query: 1   MSQVVATRSIHSTVPCHGSGSVLDRVNRARSFGIGSKVLAHEKKKRNFVCRRSLIIAKRA 60
           MSQVVATRSIHSTVPCHGSGSVLDRVNRARSFGIGSKVLAHEKKKRNFVCRRSLI AKRA
Sbjct: 1   MSQVVATRSIHSTVPCHGSGSVLDRVNRARSFGIGSKVLAHEKKKRNFVCRRSLITAKRA 60

Query: 61  AQAEVVPVSPEDIQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
           AQ EVVPVSPEDIQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE
Sbjct: 61  AQVEVVPVSPEDIQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQCKDNCIAIMLDTKGPEVRSGDL 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQCKDNCIAIMLDTKGPEVRSGDL
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQCKDNCIAIMLDTKGPEVRSGDL 180

Query: 181 PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDILLVDGGMMSFMVKSKTED 240
           PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDILLVDGGMMSFMVKSKTED
Sbjct: 181 PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDILLVDGGMMSFMVKSKTED 240

Query: 241 SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300
           SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV
Sbjct: 241 SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300

Query: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360
           HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE
Sbjct: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360

Query: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420
           EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP
Sbjct: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420

Query: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTKT 480
           LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTKT
Sbjct: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTKT 480

Query: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ 540
           GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ
Sbjct: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ 540

Query: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573
           GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Sbjct: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573

BLAST of Csor.00g305350.m01 vs. ExPASy TrEMBL
Match: A0A6J1HTY0 (Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111467769 PE=3 SV=1)

HSP 1 Score: 1123 bits (2904), Expect = 0.0
Identity = 570/573 (99.48%), Postives = 571/573 (99.65%), Query Frame = 0

Query: 1   MSQVVATRSIHSTVPCHGSGSVLDRVNRARSFGIGSKVLAHEKKKRNFVCRRSLIIAKRA 60
           MSQVVATRSIHSTVPCHGSGSVLDRVNRARSFGIGSKVLAHEKKKRNFVCRRSLI AKRA
Sbjct: 1   MSQVVATRSIHSTVPCHGSGSVLDRVNRARSFGIGSKVLAHEKKKRNFVCRRSLITAKRA 60

Query: 61  AQAEVVPVSPEDIQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
           AQAEVVPVSPEDIQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE
Sbjct: 61  AQAEVVPVSPEDIQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQCKDNCIAIMLDTKGPEVRSGDL 180
           MIWKLAEAGMNVARLNMSHGDHASH KVIDLVREYNAQCKDNCIAIMLDTKGPEVRSGDL
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHHKVIDLVREYNAQCKDNCIAIMLDTKGPEVRSGDL 180

Query: 181 PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDILLVDGGMMSFMVKSKTED 240
           PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGD+LLVDGGMMSFMVKSKTED
Sbjct: 181 PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDMLLVDGGMMSFMVKSKTED 240

Query: 241 SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300
           SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV
Sbjct: 241 SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300

Query: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360
           HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE
Sbjct: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360

Query: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420
           EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP
Sbjct: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420

Query: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTKT 480
           LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTKT
Sbjct: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTKT 480

Query: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ 540
           GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ
Sbjct: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ 540

Query: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573
           GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Sbjct: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573

BLAST of Csor.00g305350.m01 vs. ExPASy TrEMBL
Match: A0A6J1J4F4 (Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111482565 PE=3 SV=1)

HSP 1 Score: 1072 bits (2773), Expect = 0.0
Identity = 544/573 (94.94%), Postives = 558/573 (97.38%), Query Frame = 0

Query: 1   MSQVVATRSIHSTVPCHGSGSVLDRVNRARSFGIGSKVLAHEKKKRNFVCRRSLIIAKRA 60
           MSQVVATRSIHST  C  SGSV DRV+RARSFGIGSK+L HEKK  NF  RRSLI +K A
Sbjct: 1   MSQVVATRSIHSTFLCPSSGSVHDRVDRARSFGIGSKLLRHEKKSWNFAYRRSLITSKGA 60

Query: 61  AQAEVVPVSPEDIQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
            QAEVVPVSPED+QKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPST+TKE
Sbjct: 61  TQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTDTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQCKDNCIAIMLDTKGPEVRSGDL 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQ +DNCIAIMLDTKGPEVRSGDL
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180

Query: 181 PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDILLVDGGMMSFMVKSKTED 240
           PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGD++LVDGGMMS MVKSKTED
Sbjct: 181 PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDMILVDGGMMSLMVKSKTED 240

Query: 241 SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300
           SVKCEV+DGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV
Sbjct: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300

Query: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360
           HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE
Sbjct: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360

Query: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420
           EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP
Sbjct: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420

Query: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTKT 480
           LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFT+T
Sbjct: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480

Query: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ 540
           GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTF+DALS+LQRQ
Sbjct: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFSDALSMLQRQ 540

Query: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573
           GMVKEGEEVAL+QSG+QPIWRFPSTHNIQVRKV
Sbjct: 541 GMVKEGEEVALVQSGKQPIWRFPSTHNIQVRKV 573

BLAST of Csor.00g305350.m01 vs. ExPASy TrEMBL
Match: A0A6J1E5B9 (Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111430719 PE=3 SV=1)

HSP 1 Score: 1072 bits (2773), Expect = 0.0
Identity = 543/573 (94.76%), Postives = 558/573 (97.38%), Query Frame = 0

Query: 1   MSQVVATRSIHSTVPCHGSGSVLDRVNRARSFGIGSKVLAHEKKKRNFVCRRSLIIAKRA 60
           MSQVVATRSIHST  C  SGSV DRV+RARSFGIGSK+L HEKK  NF  RRSLI +K A
Sbjct: 1   MSQVVATRSIHSTFLCPSSGSVQDRVDRARSFGIGSKLLGHEKKSWNFAYRRSLITSKGA 60

Query: 61  AQAEVVPVSPEDIQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
            QAEVVPVSPED+QKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPST+TKE
Sbjct: 61  TQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTDTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQCKDNCIAIMLDTKGPEVRSGDL 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQ +DNCIAIMLDTKGPEVRSGDL
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180

Query: 181 PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDILLVDGGMMSFMVKSKTED 240
           PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGD++LVDGGMMS MVKSKTED
Sbjct: 181 PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDMILVDGGMMSLMVKSKTED 240

Query: 241 SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300
           SVKCEV+DGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV
Sbjct: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300

Query: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360
           HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE
Sbjct: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360

Query: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420
           EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP
Sbjct: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420

Query: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTKT 480
           LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFT+T
Sbjct: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480

Query: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ 540
           GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCP+YMQFSDDAEQTF+DALS+LQRQ
Sbjct: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPLYMQFSDDAEQTFSDALSMLQRQ 540

Query: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573
           GMVKEGEEVAL+QSG+QPIWRFPSTHNIQVRKV
Sbjct: 541 GMVKEGEEVALVQSGKQPIWRFPSTHNIQVRKV 573

BLAST of Csor.00g305350.m01 vs. ExPASy TrEMBL
Match: A0A1S3AY45 (Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103484139 PE=3 SV=1)

HSP 1 Score: 1072 bits (2773), Expect = 0.0
Identity = 545/573 (95.11%), Postives = 558/573 (97.38%), Query Frame = 0

Query: 1   MSQVVATRSIHSTVPCHGSGSVLDRVNRARSFGIGSKVLAHEKKKRNFVCRRSLIIAKRA 60
           MSQVVATRSIHST  C  SGSV +R++RARSFG GSKVLAHEKK  N   RRSLI +KRA
Sbjct: 1   MSQVVATRSIHSTFLCPSSGSVQERLDRARSFGTGSKVLAHEKKSWNLSYRRSLITSKRA 60

Query: 61  AQAEVVPVSPEDIQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
           AQAEVVPVSPED+QKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE
Sbjct: 61  AQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQCKDNCIAIMLDTKGPEVRSGDL 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQ +DNCIAIMLDTKGPEVRSGDL
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180

Query: 181 PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDILLVDGGMMSFMVKSKTED 240
           PQPILLE+GQEFTFTIRRGVSTAD+VSVNYDDFVNDVEVGD+LLVDGGMMS MVKSKTED
Sbjct: 181 PQPILLETGQEFTFTIRRGVSTADTVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240

Query: 241 SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300
           SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV
Sbjct: 241 SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300

Query: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360
           HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE
Sbjct: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360

Query: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420
           EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP
Sbjct: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420

Query: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTKT 480
           LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFT+T
Sbjct: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480

Query: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ 540
           GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFS+DAEQTF DAL++LQRQ
Sbjct: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSEDAEQTFTDALTMLQRQ 540

Query: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573
           GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Sbjct: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573

BLAST of Csor.00g305350.m01 vs. TAIR 10
Match: AT5G52920.1 (plastidic pyruvate kinase beta subunit 1 )

HSP 1 Score: 912.1 bits (2356), Expect = 2.2e-265
Identity = 463/579 (79.97%), Postives = 506/579 (87.39%), Query Frame = 0

Query: 1   MSQVVATRSIHSTVPCHGSGSVLDRVNR-ARSFGIGSKVLAHEKKKRNFVCRRS-----L 60
           M+QVVATRSI  ++     GSV  R  +  +      KVL +E K+   V  RS      
Sbjct: 1   MAQVVATRSIQGSMLSPNGGSVSTRSEKLLKPASFAVKVLGNEAKRSGRVSVRSRRVVDT 60

Query: 61  IIAKRAAQAEVVPVSPEDIQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGP 120
            +     + EV+PVSPED+   +EQ E L  +QQ GDT VGMWSKPTVRRKTKIVCT+GP
Sbjct: 61  TVRSARVETEVIPVSPEDVPNREEQLERLLEMQQFGDTSVGMWSKPTVRRKTKIVCTVGP 120

Query: 121 STNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQCKDNCIAIMLDTKGPE 180
           STNT+EMIWKLAEAGMNVAR+NMSHGDHASH+KVIDLV+EYNAQ KDN IAIMLDTKGPE
Sbjct: 121 STNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNTIAIMLDTKGPE 180

Query: 181 VRSGDLPQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDILLVDGGMMSFMV 240
           VRSGDLPQPI+L+ GQEFTFTI RGVST   VSVNYDDFVNDVE GD+LLVDGGMMSFMV
Sbjct: 181 VRSGDLPQPIMLDPGQEFTFTIERGVSTPSCVSVNYDDFVNDVEAGDMLLVDGGMMSFMV 240

Query: 241 KSKTEDSVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFV 300
           KSKT+DSVKCEV+DGGELKSRRHLNVRGKSATLPSITEKDW+DIKFGV+NKVDFYAVSFV
Sbjct: 241 KSKTKDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWEDIKFGVENKVDFYAVSFV 300

Query: 301 KDAQVVHELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEE 360
           KDAQVVHELKKYL++  ADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEE
Sbjct: 301 KDAQVVHELKKYLQNSGADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEE 360

Query: 361 VPLLQEEIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGET 420
           VP+LQEEIINLCR MGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADA+MLSGET
Sbjct: 361 VPILQEEIINLCRSMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGET 420

Query: 421 AHGKFPLKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSI 480
           AHGKFPLKA  VMHTVALRTEATI  G MP NLGQ FKNHMSEMFAYHATMMSNTLGTS 
Sbjct: 421 AHGKFPLKAAGVMHTVALRTEATITSGEMPPNLGQAFKNHMSEMFAYHATMMSNTLGTST 480

Query: 481 VVFTKTGFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFADAL 540
           VVFT+TGFMAILLSHYRPSGT +AFTN+K+IQQRLALYQGVCPIYM+F+DDAE+TFA+AL
Sbjct: 481 VVFTRTGFMAILLSHYRPSGTIYAFTNEKKIQQRLALYQGVCPIYMEFTDDAEETFANAL 540

Query: 541 SLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           + L +QGMVK+GEE+A++QSG QPIWR  STHNIQVRKV
Sbjct: 541 ATLLKQGMVKKGEEIAIVQSGTQPIWRSQSTHNIQVRKV 579

BLAST of Csor.00g305350.m01 vs. TAIR 10
Match: AT1G32440.1 (plastidial pyruvate kinase 3 )

HSP 1 Score: 729.2 bits (1881), Expect = 2.6e-210
Identity = 370/471 (78.56%), Postives = 410/471 (87.05%), Query Frame = 0

Query: 103 RRKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQCKDN 162
           RRKTKIVCTIGPS++++EMIWKLAEAGMNVARLNMSHGDHASHQ  IDLV+EYN+   D 
Sbjct: 98  RRKTKIVCTIGPSSSSREMIWKLAEAGMNVARLNMSHGDHASHQITIDLVKEYNSLFVDK 157

Query: 163 CIAIMLDTKGPEVRSGDLPQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDI 222
            IAIMLDTKGPEVRSGD+PQPI LE GQEF FTI+RGVS  D+VSVNYDDFVNDVEVGDI
Sbjct: 158 AIAIMLDTKGPEVRSGDVPQPIFLEEGQEFNFTIKRGVSLKDTVSVNYDDFVNDVEVGDI 217

Query: 223 LLVDGGMMSFMVKSKTEDSVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGV 282
           LLVDGGMMS  VKSKT D VKC VIDGGEL+SRRHLNVRGKSATLPSIT+KDW+DIKFGV
Sbjct: 218 LLVDGGMMSLAVKSKTSDLVKCVVIDGGELQSRRHLNVRGKSATLPSITDKDWEDIKFGV 277

Query: 283 DNKVDFYAVSFVKDAQVVHELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVA 342
           DN+VDFYAVSFVKDA+VVHELK YLK+C ADI VIVKIESADSI NL SII+A DGAMVA
Sbjct: 278 DNQVDFYAVSFVKDAKVVHELKNYLKTCSADISVIVKIESADSIKNLPSIISACDGAMVA 337

Query: 343 RGDLGAELPIEEVPLLQEEIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVR 402
           RGDLGAELPIEEVPLLQEEII  CR + K VIVATNMLESMI HPTPTRAEVSDIAIAVR
Sbjct: 338 RGDLGAELPIEEVPLLQEEIIRRCRSIHKPVIVATNMLESMINHPTPTRAEVSDIAIAVR 397

Query: 403 EGADAIMLSGETAHGKFPLKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYH 462
           EGADAIMLSGETAHGKFPLKAV VMHTVALRTEA++   R   +    +K HM +MFA+H
Sbjct: 398 EGADAIMLSGETAHGKFPLKAVNVMHTVALRTEASLP-VRTSASRTTAYKGHMGQMFAFH 457

Query: 463 ATMMSNTLGTSIVVFTKTGFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQF 522
           A++M+NTL + ++VFT+TG MA+LLSHYRPS T FAFTN +RI QRLALYQGV PIYM+F
Sbjct: 458 ASIMANTLSSPLIVFTRTGSMAVLLSHYRPSATIFAFTNQRRIMQRLALYQGVMPIYMEF 517

Query: 523 SDDAEQTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           SDDAE T+A +L LLQ + M+KEG+ V L+QSG QPIWR  STH IQVRK+
Sbjct: 518 SDDAEDTYARSLKLLQDENMLKEGQHVTLVQSGSQPIWREESTHLIQVRKI 567

BLAST of Csor.00g305350.m01 vs. TAIR 10
Match: AT3G22960.1 (Pyruvate kinase family protein )

HSP 1 Score: 379.0 bits (972), Expect = 6.6e-105
Identity = 224/475 (47.16%), Postives = 299/475 (62.95%), Query Frame = 0

Query: 104 RKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQCKDNC 163
           R+TK++CTIGP+T   E +  LA  GMNVARLNM HG    H+ VI  VR  N + K   
Sbjct: 117 RRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHRGVIRSVRRLNEE-KGFA 176

Query: 164 IAIMLDTKGPEVRSGDL--PQPILLESGQEFTFTIRRGVSTAD--SVSVNYDDFVNDVEV 223
           +AIM+DT+G E+  GDL        E G+ +TFT+R   S+    ++SV+YD F  DV V
Sbjct: 177 VAIMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPERTISVSYDGFAEDVRV 236

Query: 224 GDILLVDGGMMSFMVKSKTEDSVKCEVIDGGELKSRRHLN-------VRGKSATLPSITE 283
           GD LLVDGGM+ F V  K    VKC   D G L  R +L        VR ++A LP+I+ 
Sbjct: 237 GDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGSLVRERNAMLPTISS 296

Query: 284 KDWDDIKFGVDNKVDFYAVSFVKDAQVVHELKKYL--KSCDADIHVIVKIESADSIPNLH 343
           KDW DI FG+   VDF AVSFVK A+V++ LK YL  +S   +I VI KIES DS+ NL 
Sbjct: 297 KDWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEIGVIAKIESIDSLTNLE 356

Query: 344 SIITASDGAMVARGDLGAELPIEEVPLLQEEIINLCRGMGKAVIVATNMLESMIVHPTPT 403
            II ASDGAMVARGDLGA++P+E+VP  Q+ I+ +CR + K VIVA+ +LESMI +PTPT
Sbjct: 357 EIILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVIVASQLLESMIEYPTPT 416

Query: 404 RAEVSDIAIAVREGADAIMLSGETAHGKFPLKAVKVMHTVALRTEATIEGGR----MPLN 463
           RAEV+D++ AVR+ +DA+MLSGE+A G+FP KA+ V+ TV+LR E      +    +PL 
Sbjct: 417 RAEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRIERWWREEKRHESVPLQ 476

Query: 464 -LGQTFKNHMSEMFAYHATMMSNTLGT-SIVVFTKTGFMAILLSHYRPSGTTFAFTNDKR 523
            +G +F + +SE     A  M+N LG  ++ V+T +G MA L+S  RP    FAFT    
Sbjct: 477 AIGSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVSRCRPDCPIFAFTTTTS 536

Query: 524 IQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQGMVKEGEEVALLQSGRQPI 560
           +++RL L  G+ P  + FSDD E       SLL+ +GM+K G+ V  +    Q I
Sbjct: 537 VRRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDLVIAVSDMLQSI 590

BLAST of Csor.00g305350.m01 vs. TAIR 10
Match: AT3G25960.1 (Pyruvate kinase family protein )

HSP 1 Score: 275.4 bits (703), Expect = 1.0e-73
Identity = 171/477 (35.85%), Postives = 265/477 (55.56%), Query Frame = 0

Query: 95  GMWSKPTVRRKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVRE 154
           G  +   +R KTKIVCT+GP++ + EMI KL +AGMNVAR N SHG H+ HQ+ +D +R 
Sbjct: 7   GQATNGALRSKTKIVCTLGPASRSVEMIEKLLKAGMNVARFNFSHGSHSYHQETLDNLRT 66

Query: 155 YNAQCKDNCIAIMLDTKGPEVRSGDLPQ--PILLESGQEFTFTIRRGV-STADSVSVNYD 214
                   C A+MLDTK P +R+G L +  PI L+ GQE T +I   +   ++++S++Y 
Sbjct: 67  AMDNTGILC-AVMLDTKSPVIRTGFLKEGKPIQLKQGQEITISIDYKIQGDSNTISMSYK 126

Query: 215 DFVNDVEVGDILLVDGGMMSFMVKS--KTEDSVKCEVIDGGELKSRRHLNVRGKSATLPS 274
               D++ GD++L   G +S  V S  K    V+C   +   L  R+++N+ G    LP+
Sbjct: 127 KLAEDLKPGDVILCSDGTISLNVLSCDKYLGLVRCRCENSALLGERKNVNLPGIVVDLPT 186

Query: 275 ITEKDWDDI-KFGVDNKVDFYAVSFVKDAQVVHELKKYLKSCDADIHVIVKIESADSIPN 334
           +TEKD +DI ++GV NK+D  A+SFV+    + +++K L      I ++ K+E+ + + N
Sbjct: 187 LTEKDKEDIMQWGVPNKIDIIALSFVRKGSDLIQVRKLLGEHSKSIMLMSKVENQEGVMN 246

Query: 335 LHSIITASDGAMVARGDLGAELPIEEVPLLQEEIINLCRGMGKAVIVATNMLESMIVHPT 394
              I+  SD  MVARGDLG E+PIE++ L Q+ +IN     GK V+ AT MLESM V P 
Sbjct: 247 FDKILENSDAFMVARGDLGMEIPIEKMFLAQKTMINKANAHGKPVVTATQMLESMTVSPR 306

Query: 395 PTRAEVSDIAIAVREGADAIMLSGETAHGKFPLKAVKVMHTVALRTEATIEGG---RMPL 454
           PTRAE +D+A AV +G D +MLSGETA G  P  AV  M  +    E  I+     +  L
Sbjct: 307 PTRAEATDVANAVLDGTDCVMLSGETAAGAHPETAVLTMSRICKEAEDFIDYDILHKKTL 366

Query: 455 NLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTKTGFMAILLSHYRPSGTTFAFTNDK-- 514
            +     + +  + A   +   +   ++IVV T+ G+ A L++ YRPS    +    +  
Sbjct: 367 GMVSLPLSPIESLAASAVSTARSVFASAIVVLTRGGYTAELVAKYRPSVPILSVIMPEIA 426

Query: 515 -------RIQQRLALYQGVCPIY-MQFSDDAEQTFADALSLLQRQGMVKEGEEVALL 553
                   + +R  +Y+G+ P+      D  E+    A+   + +G+ K G+ +  L
Sbjct: 427 ECSDSVAHVARRGLIYRGIIPVVGCSARDSTEEMIRLAIGFAKTKGICKTGDSIVAL 482

BLAST of Csor.00g305350.m01 vs. TAIR 10
Match: AT3G55650.1 (Pyruvate kinase family protein )

HSP 1 Score: 273.9 bits (699), Expect = 3.0e-73
Identity = 177/493 (35.90%), Postives = 269/493 (54.56%), Query Frame = 0

Query: 95  GMWSKPTVRRKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVRE 154
           G  +   +R KTKI+CT+GP + + EMI KL +AGMNVAR N SHG H+ HQ+ +D +R 
Sbjct: 7   GQATNGALRSKTKIICTLGPVSRSVEMIEKLLKAGMNVARFNFSHGSHSYHQETLDNLR- 66

Query: 155 YNAQCKDN---CIAIMLDTKGPEVRSGDLPQ--PILLESGQEFTFTIRRGV-STADSVSV 214
                 DN     A+MLDTKGPE+R+G L +  PI L  GQE T +I   +   ++ +S+
Sbjct: 67  ---TAMDNTGILSAVMLDTKGPEIRTGFLKEGKPIQLNQGQEITISIDYMIEGDSNVISM 126

Query: 215 NYDDFVNDVEVGDILLVDGGMMSFMVKS--KTEDSVKCEVIDGGELKSRRHLNVRGKSAT 274
           +Y     DV+ GD++L   G +S  V S  K+   V+C   +   L  R+++N+ G    
Sbjct: 127 SYKKLAEDVKPGDVILCSDGTISLTVLSCDKSFGLVRCRCENSAILGERKNVNLPGIVVD 186

Query: 275 LPSITEKDWDD-IKFGVDNKVDFYAVSFVKDAQVVHELKKYLKSCDADIHVIVKIESADS 334
           LP++TEKD +D I++GV NK+D  A+SFV+    + E+++ L     +I ++ K+E+ + 
Sbjct: 187 LPTLTEKDKEDIIQWGVPNKIDIIALSFVRKGSDLTEVRRLLGEHSKNIMLMSKVENQEG 246

Query: 335 IPNLHSIITASDGAMVARGDLGAELPIEEVPLLQEEIINLCRGMGKAVIVATNMLESMIV 394
           + N   I+  SD  MVARGDLG E+PIE++ L Q+ +I +   +GK V+ AT MLESM V
Sbjct: 247 VMNCEKILENSDAFMVARGDLGMEIPIEKMFLAQKTMIKMANALGKPVVTATQMLESMTV 306

Query: 395 HPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFPLKAVKVMHTVALRTEATIEGG---R 454
            P PTRAE +D+A AV +G D +MLSGETA G  P  AV  M  +    E  I+     +
Sbjct: 307 SPRPTRAEATDVANAVLDGTDCVMLSGETAAGAHPEAAVLTMSRICKEAEDFIDYDILHK 366

Query: 455 MPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTKTGFMAILLSHYRPS-------GT 514
             L +     + +  + A   +   +   ++IVV TK G+ A L++ YRPS         
Sbjct: 367 KTLGMVSLPLSPIESLAASVVSTAQSVFASAIVVLTKGGYTAELVAKYRPSVPILSVIVP 426

Query: 515 TFAFTNDKRIQ---------QRLALYQGVCPIYMQFS-------DDAEQTFADALSLLQR 553
             A  ND  +          +R  +Y+ + P+    S       D  E+    A+   + 
Sbjct: 427 EIAQGNDMEMSCSDSVAHAARRGLIYRRIIPVVATGSSARDSNKDATEEMINLAIGFAKT 486

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FLW93.1e-26479.97Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1[more]
Q405464.6e-21278.09Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q93Z533.7e-20978.56Plastidial pyruvate kinase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=P... [more]
P559643.2e-18177.51Pyruvate kinase isozyme G, chloroplastic (Fragment) OS=Ricinus communis OX=3988 ... [more]
Q431171.9e-10446.64Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAG6574217.10.0100.00Plastidial pyruvate kinase 2, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7013286.10.099.83Plastidial pyruvate kinase 2 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022945683.10.099.65plastidial pyruvate kinase 2-like [Cucurbita moschata][more]
XP_022968597.10.099.48plastidial pyruvate kinase 2-like [Cucurbita maxima][more]
XP_023541338.10.099.13plastidial pyruvate kinase 2-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1G1L00.099.65Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111449841 PE=3 SV=1[more]
A0A6J1HTY00.099.48Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111467769 PE=3 SV=1[more]
A0A6J1J4F40.094.94Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111482565 PE=3 SV=1[more]
A0A6J1E5B90.094.76Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111430719 PE=3 SV=1[more]
A0A1S3AY450.095.11Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103484139 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G52920.12.2e-26579.97plastidic pyruvate kinase beta subunit 1 [more]
AT1G32440.12.6e-21078.56plastidial pyruvate kinase 3 [more]
AT3G22960.16.6e-10547.16Pyruvate kinase family protein [more]
AT3G25960.11.0e-7335.85Pyruvate kinase family protein [more]
AT3G55650.13.0e-7335.90Pyruvate kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001697Pyruvate kinasePRINTSPR01050PYRUVTKNASEcoord: 368..392
score: 62.22
coord: 412..428
score: 55.66
coord: 162..178
score: 56.29
coord: 393..411
score: 63.81
coord: 286..300
score: 42.56
coord: 343..367
score: 52.86
coord: 316..342
score: 45.81
IPR001697Pyruvate kinaseTIGRFAMTIGR01064TIGR01064coord: 104..570
e-value: 2.6E-154
score: 512.2
IPR001697Pyruvate kinasePANTHERPTHR11817PYRUVATE KINASEcoord: 35..573
IPR036918Pyruvate kinase, C-terminal domain superfamilyGENE3D3.40.1380.20coord: 446..573
e-value: 9.4E-27
score: 95.0
IPR036918Pyruvate kinase, C-terminal domain superfamilySUPERFAMILY52935PK C-terminal domain-likecoord: 422..573
IPR015793Pyruvate kinase, barrelPFAMPF00224PKcoord: 104..439
e-value: 3.8E-117
score: 391.1
IPR015795Pyruvate kinase, C-terminalPFAMPF02887PK_Ccoord: 460..553
e-value: 1.1E-14
score: 54.6
IPR040442Pyruvate kinase-like domain superfamilyGENE3D3.20.20.60coord: 106..431
e-value: 2.5E-131
score: 439.4
IPR015806Pyruvate kinase, insert domain superfamilyGENE3D2.40.33.10coord: 174..267
e-value: 2.5E-131
score: 439.4
NoneNo IPR availablePANTHERPTHR11817:SF2PLASTIDIAL PYRUVATE KINASE 2coord: 35..573
IPR018209Pyruvate kinase, active sitePROSITEPS00110PYRUVATE_KINASEcoord: 314..326
IPR011037Pyruvate kinase-like, insert domain superfamilySUPERFAMILY50800PK beta-barrel domain-likecoord: 173..264
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domain superfamilySUPERFAMILY51621Phosphoenolpyruvate/pyruvate domaincoord: 95..438

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Csor.00g305350Csor.00g305350gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g305350.m01.CDS01Csor.00g305350.m01.CDS01CDS
Csor.00g305350.m01.CDS02Csor.00g305350.m01.CDS02CDS
Csor.00g305350.m01.CDS03Csor.00g305350.m01.CDS03CDS
Csor.00g305350.m01.CDS04Csor.00g305350.m01.CDS04CDS
Csor.00g305350.m01.CDS05Csor.00g305350.m01.CDS05CDS
Csor.00g305350.m01.CDS06Csor.00g305350.m01.CDS06CDS
Csor.00g305350.m01.CDS07Csor.00g305350.m01.CDS07CDS
Csor.00g305350.m01.CDS08Csor.00g305350.m01.CDS08CDS
Csor.00g305350.m01.CDS09Csor.00g305350.m01.CDS09CDS
Csor.00g305350.m01.CDS10Csor.00g305350.m01.CDS10CDS
Csor.00g305350.m01.CDS11Csor.00g305350.m01.CDS11CDS
Csor.00g305350.m01.CDS12Csor.00g305350.m01.CDS12CDS


The following start_codon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g305350.m01.start_codon01Csor.00g305350.m01.start_codon01start_codon


The following initial feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g305350.m01.initial01Csor.00g305350.m01.initial01initial


The following intron feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g305350.m01.intron01Csor.00g305350.m01.intron01intron
Csor.00g305350.m01.intron02Csor.00g305350.m01.intron02intron
Csor.00g305350.m01.intron03Csor.00g305350.m01.intron03intron
Csor.00g305350.m01.intron04Csor.00g305350.m01.intron04intron
Csor.00g305350.m01.intron05Csor.00g305350.m01.intron05intron
Csor.00g305350.m01.intron06Csor.00g305350.m01.intron06intron
Csor.00g305350.m01.intron07Csor.00g305350.m01.intron07intron
Csor.00g305350.m01.intron08Csor.00g305350.m01.intron08intron
Csor.00g305350.m01.intron09Csor.00g305350.m01.intron09intron
Csor.00g305350.m01.intron10Csor.00g305350.m01.intron10intron
Csor.00g305350.m01.intron11Csor.00g305350.m01.intron11intron


The following internal feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g305350.m01.internal01Csor.00g305350.m01.internal01internal
Csor.00g305350.m01.internal02Csor.00g305350.m01.internal02internal
Csor.00g305350.m01.internal03Csor.00g305350.m01.internal03internal
Csor.00g305350.m01.internal04Csor.00g305350.m01.internal04internal
Csor.00g305350.m01.internal05Csor.00g305350.m01.internal05internal
Csor.00g305350.m01.internal06Csor.00g305350.m01.internal06internal
Csor.00g305350.m01.internal07Csor.00g305350.m01.internal07internal
Csor.00g305350.m01.internal08Csor.00g305350.m01.internal08internal
Csor.00g305350.m01.internal09Csor.00g305350.m01.internal09internal
Csor.00g305350.m01.internal10Csor.00g305350.m01.internal10internal


The following terminal feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g305350.m01.terminal01Csor.00g305350.m01.terminal01terminal


The following stop_codon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g305350.m01.stop_codon01Csor.00g305350.m01.stop_codon01stop_codon


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Csor.00g305350.m01Csor.00g305350.m01-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
molecular_function GO:0003824 catalytic activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0030955 potassium ion binding
molecular_function GO:0004743 pyruvate kinase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0016301 kinase activity