Csor.00g259820.m01 (mRNA) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g259820.m01
TypemRNA
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionPectinesterase
LocationCsor_Chr17: 1862158 .. 1863978 (-)
Sequence length1740
RNA-Seq ExpressionCsor.00g259820.m01
SyntenyCsor.00g259820.m01
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintronterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCAACGGATTTGATGCCGAAAGCGACAAAAAGAAGAAGAAAATCGCCGTCATTAGCGTTTCTTCTTTAATCCTCGTCGCTATGGTGGTTGCCGTCACCGTCGGCGTCAACAACGCTCCTCAAGAGGATCTCGACGACGGCGGCGGAGGCGGAGGCGGCGACGCTCATGATCACGGTGACGACGGCGAGAAGGTTTCGTCATCAACTAAAGCAATCAAAACCATTTGTCAACCAACGGATTACAAAGAAACTTGCGAAGAGAGTTTAATGGTGGAAGCCGGCAACACAACCGACCCGAAAGAGCTTGTCAAAGCCGGGTTCAAAATCGCCGTGGAAAAACTTAACGCGGCGATTAGAAATTCCACTACGCTTCAAGAATTAGCTCAAGACCCAAGAGCCAACCAAGCTCTTCAAAATTGCCGCGAGTTATTGGAATACGCCATCGACGATCTCCACGATTCTTACCATCGAATCGGCACCTTCCACGTGTCGAAATTTGAGGAGTTTGTTAACGATTTAAAGATTTGGCTTAGTGCAGCTCTCACTTATGAACAAACTTGTCTTGACGGATTTGAAAACACTACCGGCGATTCCGGAGAGAGAATGAAAGAATTTTTGAAGTCCGCTCAAGAAATGACCACTAACGGCCTTGGTATTGTCAGTGAATTGTCCAAAATTTTGGGTACACTTCACGCGCCGGCAGCGACTGGGCGGCGACTTTTGGACACTGACATGCCGTTGTGGGTTAGCGACGGGAAGCGGCGGCTAATGCAAATGGGGACGGGGGCGATTAAGCCCGACCTGGTGGTGGCTCAAGATGGGAGCGGAAAGTACAAGACAATAAATGAAGCTTTGGTTCATGTCCCGATGAAGAGTAATAAAACGTTTGTGATTTATGTCAAAGCCGGAATTTATAAGGAAATTGTTGTTATTCCTAAGCATATGACTCATTTGACCATGTATGGCGACGGACCGACCAAATCTGTCGTTACCGGTAATTTAAATTTTGTCGATGGCATTCAAACTTTCAAGACAGCTACATTCGGTAAGTATCGATGAATTTATTTATTATTATCATTATCGTATTAAGCTTTTGGATTGATTGATGATTTAACGACTATTCCAGCTGCTATTGGAGCTAATTTCTTCGTTCGAGACATGGGGTTCGTGAACACTGCTGGACCGACAAAGCACCAAGCTGTAGCCCTACGTGTCCAATCGGATCGAGCCATCATTTACAATTGTCAAATAGATGGGTACCAAGACACTCTCTACGCCCATGCTAACCGTCAGTTCTATCGAGACTGCATAATCAGTGGCACCATTGATTTCGTGTTTGGCAACTCTGCAGCTATCTTCCAAAACTGCAAGCTAGTGGTTCGTAAGCCATTGGATAATCAACAATGCATTGTCACAGCTCACGGGCGACTCAATCGTCGTGAACCGACGGCGTTGATCTTCCAAGGCTGCCACTTCATGGGCGACCCGTCTTACTTGCCGTTCAAGAAAGTGAACAAGGCGTATTTGGGGCGGCCATGGAAGCAATATTCACGAACCATTATAATGGGGTCTCAAATCGACGACTTGATCCAGCCGGAAGGATGGTTGCCGTGGATGGGCGATTTTGCACTCAATACGTTGTTCTACGCCGAGGTACAGAACAGAGGGCCAGGGGCTGATCAGAGCCATAGAGTAAAGTGGCGAGGAATTAGACATATTACGCCGCAGCACGCCGCGGCTTACACTGCCAGACGATTTATTGACGGCGATGCTTGGATTCCAGTCAGAGGGGTCCCCTACTCTTCTGGAATGATGTAA

mRNA sequence

ATGTCCAACGGATTTGATGCCGAAAGCGACAAAAAGAAGAAGAAAATCGCCGTCATTAGCGTTTCTTCTTTAATCCTCGTCGCTATGGTGGTTGCCGTCACCGTCGGCGTCAACAACGCTCCTCAAGAGGATCTCGACGACGGCGGCGGAGGCGGAGGCGGCGACGCTCATGATCACGGTGACGACGGCGAGAAGGTTTCGTCATCAACTAAAGCAATCAAAACCATTTGTCAACCAACGGATTACAAAGAAACTTGCGAAGAGAGTTTAATGGTGGAAGCCGGCAACACAACCGACCCGAAAGAGCTTGTCAAAGCCGGGTTCAAAATCGCCGTGGAAAAACTTAACGCGGCGATTAGAAATTCCACTACGCTTCAAGAATTAGCTCAAGACCCAAGAGCCAACCAAGCTCTTCAAAATTGCCGCGAGTTATTGGAATACGCCATCGACGATCTCCACGATTCTTACCATCGAATCGGCACCTTCCACGTGTCGAAATTTGAGGAGTTTGTTAACGATTTAAAGATTTGGCTTAGTGCAGCTCTCACTTATGAACAAACTTGTCTTGACGGATTTGAAAACACTACCGGCGATTCCGGAGAGAGAATGAAAGAATTTTTGAAGTCCGCTCAAGAAATGACCACTAACGGCCTTGGTATTGTCAGTGAATTGTCCAAAATTTTGGGTACACTTCACGCGCCGGCAGCGACTGGGCGGCGACTTTTGGACACTGACATGCCGTTGTGGGTTAGCGACGGGAAGCGGCGGCTAATGCAAATGGGGACGGGGGCGATTAAGCCCGACCTGGTGGTGGCTCAAGATGGGAGCGGAAAGTACAAGACAATAAATGAAGCTTTGGTTCATGTCCCGATGAAGAGTAATAAAACGTTTGTGATTTATGTCAAAGCCGGAATTTATAAGGAAATTGTTGTTATTCCTAAGCATATGACTCATTTGACCATGTATGGCGACGGACCGACCAAATCTGTCGTTACCGGTAATTTAAATTTTGTCGATGGCATTCAAACTTTCAAGACAGCTACATTCGCTGCTATTGGAGCTAATTTCTTCGTTCGAGACATGGGGTTCGTGAACACTGCTGGACCGACAAAGCACCAAGCTGTAGCCCTACGTGTCCAATCGGATCGAGCCATCATTTACAATTGTCAAATAGATGGGTACCAAGACACTCTCTACGCCCATGCTAACCGTCAGTTCTATCGAGACTGCATAATCAGTGGCACCATTGATTTCGTGTTTGGCAACTCTGCAGCTATCTTCCAAAACTGCAAGCTAGTGGTTCGTAAGCCATTGGATAATCAACAATGCATTGTCACAGCTCACGGGCGACTCAATCGTCGTGAACCGACGGCGTTGATCTTCCAAGGCTGCCACTTCATGGGCGACCCGTCTTACTTGCCGTTCAAGAAAGTGAACAAGGCGTATTTGGGGCGGCCATGGAAGCAATATTCACGAACCATTATAATGGGGTCTCAAATCGACGACTTGATCCAGCCGGAAGGATGGTTGCCGTGGATGGGCGATTTTGCACTCAATACGTTGTTCTACGCCGAGGTACAGAACAGAGGGCCAGGGGCTGATCAGAGCCATAGAGTAAAGTGGCGAGGAATTAGACATATTACGCCGCAGCACGCCGCGGCTTACACTGCCAGACGATTTATTGACGGCGATGCTTGGATTCCAGTCAGAGGGGTCCCCTACTCTTCTGGAATGATGTAA

Coding sequence (CDS)

ATGTCCAACGGATTTGATGCCGAAAGCGACAAAAAGAAGAAGAAAATCGCCGTCATTAGCGTTTCTTCTTTAATCCTCGTCGCTATGGTGGTTGCCGTCACCGTCGGCGTCAACAACGCTCCTCAAGAGGATCTCGACGACGGCGGCGGAGGCGGAGGCGGCGACGCTCATGATCACGGTGACGACGGCGAGAAGGTTTCGTCATCAACTAAAGCAATCAAAACCATTTGTCAACCAACGGATTACAAAGAAACTTGCGAAGAGAGTTTAATGGTGGAAGCCGGCAACACAACCGACCCGAAAGAGCTTGTCAAAGCCGGGTTCAAAATCGCCGTGGAAAAACTTAACGCGGCGATTAGAAATTCCACTACGCTTCAAGAATTAGCTCAAGACCCAAGAGCCAACCAAGCTCTTCAAAATTGCCGCGAGTTATTGGAATACGCCATCGACGATCTCCACGATTCTTACCATCGAATCGGCACCTTCCACGTGTCGAAATTTGAGGAGTTTGTTAACGATTTAAAGATTTGGCTTAGTGCAGCTCTCACTTATGAACAAACTTGTCTTGACGGATTTGAAAACACTACCGGCGATTCCGGAGAGAGAATGAAAGAATTTTTGAAGTCCGCTCAAGAAATGACCACTAACGGCCTTGGTATTGTCAGTGAATTGTCCAAAATTTTGGGTACACTTCACGCGCCGGCAGCGACTGGGCGGCGACTTTTGGACACTGACATGCCGTTGTGGGTTAGCGACGGGAAGCGGCGGCTAATGCAAATGGGGACGGGGGCGATTAAGCCCGACCTGGTGGTGGCTCAAGATGGGAGCGGAAAGTACAAGACAATAAATGAAGCTTTGGTTCATGTCCCGATGAAGAGTAATAAAACGTTTGTGATTTATGTCAAAGCCGGAATTTATAAGGAAATTGTTGTTATTCCTAAGCATATGACTCATTTGACCATGTATGGCGACGGACCGACCAAATCTGTCGTTACCGGTAATTTAAATTTTGTCGATGGCATTCAAACTTTCAAGACAGCTACATTCGCTGCTATTGGAGCTAATTTCTTCGTTCGAGACATGGGGTTCGTGAACACTGCTGGACCGACAAAGCACCAAGCTGTAGCCCTACGTGTCCAATCGGATCGAGCCATCATTTACAATTGTCAAATAGATGGGTACCAAGACACTCTCTACGCCCATGCTAACCGTCAGTTCTATCGAGACTGCATAATCAGTGGCACCATTGATTTCGTGTTTGGCAACTCTGCAGCTATCTTCCAAAACTGCAAGCTAGTGGTTCGTAAGCCATTGGATAATCAACAATGCATTGTCACAGCTCACGGGCGACTCAATCGTCGTGAACCGACGGCGTTGATCTTCCAAGGCTGCCACTTCATGGGCGACCCGTCTTACTTGCCGTTCAAGAAAGTGAACAAGGCGTATTTGGGGCGGCCATGGAAGCAATATTCACGAACCATTATAATGGGGTCTCAAATCGACGACTTGATCCAGCCGGAAGGATGGTTGCCGTGGATGGGCGATTTTGCACTCAATACGTTGTTCTACGCCGAGGTACAGAACAGAGGGCCAGGGGCTGATCAGAGCCATAGAGTAAAGTGGCGAGGAATTAGACATATTACGCCGCAGCACGCCGCGGCTTACACTGCCAGACGATTTATTGACGGCGATGCTTGGATTCCAGTCAGAGGGGTCCCCTACTCTTCTGGAATGATGTAA

Protein sequence

MSNGFDAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHGDDGEKVSSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIRNSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSAALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLHAPAATGRRLLDTDMPLWVSDGKRRLMQMGTGAIKPDLVVAQDGSGKYKTINEALVHVPMKSNKTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGANFFVRDMGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGTIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSYLPFKKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQSHRVKWRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM
Homology
BLAST of Csor.00g259820.m01 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 1.1e-181
Identity = 298/578 (51.56%), Postives = 410/578 (70.93%), Query Frame = 0

Query: 1   MSNGFDAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHG 60
           MS G+D E  K+KK+  +IS+SS++L++MVVAVT+GV+    ++  D             
Sbjct: 1   MSYGYDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAGD------------- 60

Query: 61  DDGEKVSSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIR 120
              E++++S KAIK +C PTDYKETCE++L  +A +T+DP ELVK  F   +++++   +
Sbjct: 61  ---EEITTSVKAIKDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAK 120

Query: 121 NSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSA 180
            S T+ EL +DPRA  AL  C+EL++YAI +L  S+  +G F   K +E +  L+IWLSA
Sbjct: 121 KSQTMIELQKDPRAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSA 180

Query: 181 ALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLHAPAATGRR 240
            +++EQTCLDGF+ T G++GE +K+ LK+A ++T NGL +V+E+S  LG +  P    RR
Sbjct: 181 TISHEQTCLDGFQGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRR 240

Query: 241 LLDTDMPLWVSDGKRRLMQMGTGAIKPDLVVAQDGSGKYKTINEALVHVPMKSNKTFVIY 300
           LL  + P W+    RRL+      +KPD+VVAQDGSG+YKTINEAL  VP K N TFV++
Sbjct: 241 LLSQEFPSWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVH 300

Query: 301 VKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGANFFVRD 360
           +K GIYKE V + + MTHL   GDGP K+V++G+ ++ DGI T+KTAT A +G +F  ++
Sbjct: 301 IKEGIYKEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKN 360

Query: 361 MGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGTIDFVF 420
           + F NTAG  KHQAVA+RV +D +I YNC+ DGYQDTLYAH++RQFYRDC ISGTIDF+F
Sbjct: 361 IAFENTAGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLF 420

Query: 421 GNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSYLPFKKVNK 480
           G++AA+FQNC L+VRKPL NQ C +TAHGR + RE T  + QGC  +G+P YL  K+ +K
Sbjct: 421 GDAAAVFQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSK 480

Query: 481 AYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQSHRVK 540
            YLGRPWK+YSRTIIM + I D + PEGW PW+G+F LNTLFY+EVQN GPGA  + RV 
Sbjct: 481 TYLGRPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVT 540

Query: 541 WRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGM 579
           W GI+ ++ +    +T  ++I GDAWIP +GVPY  G+
Sbjct: 541 WPGIKKLSDEEILKFTPAQYIQGDAWIPGKGVPYILGL 562

BLAST of Csor.00g259820.m01 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 635.2 bits (1637), Expect = 7.3e-181
Identity = 300/581 (51.64%), Postives = 418/581 (71.94%), Query Frame = 0

Query: 1   MSNGFDAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHG 60
           MS G+D ES K+K++  VI++SS++L++MVVAVTVGV+      L+   G   G A    
Sbjct: 1   MSYGYDDES-KRKRRYIVITISSVLLISMVVAVTVGVS------LNKHDGDSKGKA---- 60

Query: 61  DDGEKVSSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIR 120
               +V++S KA+K +C PTDY++TCE++L+    NTTDP ELVK  F + ++++  A +
Sbjct: 61  ----EVNASVKAVKDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAK 120

Query: 121 NSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSA 180
            S T+ EL +D R   AL  C+EL++YA+D+L +S+  +G F     +E + +L+IWLSA
Sbjct: 121 KSQTIMELQKDSRTRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSA 180

Query: 181 ALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLHAPAATGRR 240
           A+++E+TCL+GF+ T G++GE MK+ LK+A E+T NGL I+SE+S  +G +  P    RR
Sbjct: 181 AISHEETCLEGFQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRR 240

Query: 241 LLDTDMPLWVSDGKRRLMQMGT--GAIKPDLVVAQDGSGKYKTINEALVHVPMKSNKTFV 300
           LL    P WV    R+L+Q       +KPD+VVAQDGSG+YKTINEAL  VP K N TFV
Sbjct: 241 LLAEGFPSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFV 300

Query: 301 IYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGANFFV 360
           +++KAG+YKE V + K M+HL   GDGP K++++GN N+ DGI T++TAT A +G  F  
Sbjct: 301 VHIKAGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIA 360

Query: 361 RDMGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGTIDF 420
           +++GF NTAG  KHQAVA+RVQSD +I +NC+ DGYQDTLY H++RQF+RDC ISGTIDF
Sbjct: 361 KNIGFENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDF 420

Query: 421 VFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSYLPFKKV 480
           +FG++AA+FQNC L+VRKPL NQ C +TAHGR + RE T  +FQGC   G+P YL  K+ 
Sbjct: 421 LFGDAAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKET 480

Query: 481 NKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQSHR 540
           +KAYLGRPWK+YSRTIIM + I D +QP+GW PW+GDF L TLFY+EVQN GPG+  ++R
Sbjct: 481 SKAYLGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANR 540

Query: 541 VKWRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM 580
           V W GI+ ++ +    +T  ++I GD WIP +GVPY++G++
Sbjct: 541 VTWAGIKTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLL 566

BLAST of Csor.00g259820.m01 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 9.3e-160
Identity = 268/582 (46.05%), Postives = 382/582 (65.64%), Query Frame = 0

Query: 4   GFDAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHGDDG 63
           G D E  KKK  IA +  + L+L+ + V +T   N +  E +                  
Sbjct: 2   GVDGELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVP---------------- 61

Query: 64  EKVSSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIRNST 123
            ++ ++T A++ +C PTDYKETC  SLM  + ++T P +L+K GF + +  +  +I+ ++
Sbjct: 62  VQIKTATTAVEAVCAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKAS 121

Query: 124 T--LQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSAA 183
                + A D     AL+ C +L+  A DDL         F + + E+FV DL++WLS +
Sbjct: 122 VELTAKAANDKDTKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGS 181

Query: 184 LTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLHAPAATG--- 243
           + Y+QTC+D FE T     + M++  K+++E+T+NGL +++ +S +LG  +    TG   
Sbjct: 182 IAYQQTCMDTFEETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLG 241

Query: 244 ---RRLLDTD--MPLWVSDGKRRLMQMGTGAIKPDLVVAQDGSGKYKTINEALVHVPMKS 303
              R+LL  +  +P WV    RRLM    G +K ++VVA DGSG+YKTINEAL  VP  +
Sbjct: 242 KYARKLLSAEDGIPSWVGPNTRRLMAT-KGGVKANVVVAHDGSGQYKTINEALNAVPKAN 301

Query: 304 NKTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDG-IQTFKTATFAAI 363
            K FVIY+K G+Y E V + K MTH+T  GDGPTK+ +TG+LN+  G ++T+ TAT A  
Sbjct: 302 QKPFVIYIKQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAIN 361

Query: 364 GANFFVRDMGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCII 423
           G NF  +++GF NTAGP  HQAVALRV +D A+ YNCQIDGYQDTLY H++RQF+RDC +
Sbjct: 362 GDNFTAKNIGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTV 421

Query: 424 SGTIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSY 483
           SGT+DF+FG+   + QNC +VVRKP+ +Q C++TA GR ++RE T L+ Q CH  G+P+Y
Sbjct: 422 SGTVDFIFGDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAY 481

Query: 484 LPFKKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPG 543
           +P K +NKAYLGRPWK++SRTIIMG+ IDD+I P GWLPW GDFALNTL+YAE +N GPG
Sbjct: 482 IPVKSINKAYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPG 541

Query: 544 ADQSHRVKWRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPY 575
           ++Q+ RVKW GI+ ++P+ A  +T  RF+ G+ WIP   VPY
Sbjct: 542 SNQAQRVKWPGIKKLSPKQALRFTPARFLRGNLWIPPNRVPY 566

BLAST of Csor.00g259820.m01 vs. ExPASy Swiss-Prot
Match: Q8GXA1 (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3702 GN=PME23 PE=2 SV=3)

HSP 1 Score: 530.0 bits (1364), Expect = 3.3e-149
Identity = 267/584 (45.72%), Postives = 376/584 (64.38%), Query Frame = 0

Query: 7   AESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHGDDGEKV 66
           ++ DKKKK I   SVS   LV MVV+V V  +                  H   DD   +
Sbjct: 3   SDGDKKKKFIVAGSVSG-FLVIMVVSVAVVTSK-----------------HSPKDDENHI 62

Query: 67  SSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIRNST--T 126
             +TKA++ +C PTD+K+TC  SLM  + ++ DP +L+K GFK+ ++ +N ++  ++   
Sbjct: 63  RKTTKAVQAVCAPTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDI 122

Query: 127 LQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSAALTY 186
             +  ++P A  A + C +L+  AIDDL         F V + E FV DL++WLS ++ +
Sbjct: 123 KAKADKNPEAKGAFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAF 182

Query: 187 EQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLHAPAATG------ 246
           +QTC+D F     +  + M +  K+++E+++N L +V+ +S ++   +    TG      
Sbjct: 183 QQTCMDSFGEIKSNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYA 242

Query: 247 RRLLDTD--MPLWVSDGKRRLMQM---GTGAIKPDLVVAQDGSGKYKTINEALVHVPMKS 306
           R+LL T+  +P WV    RRLM     G G +K + VVAQDG+G++KTI +AL  VP  +
Sbjct: 243 RKLLSTEDSIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGN 302

Query: 307 NKTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNF-VDGIQTFKTATFAAI 366
              F+I++K GIYKE V + K M H+T  GDGP K+++TG+LNF +  ++TF TAT    
Sbjct: 303 KVPFIIHIKEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIE 362

Query: 367 GANFFVRDMGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCII 426
           G +F  +++G  NTAGP   QAVALRV +D A+ ++CQIDG+QDTLY H++RQFYRDC +
Sbjct: 363 GDHFTAKNIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTV 422

Query: 427 SGTIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSY 486
           SGT+DF+FG++  I QNCK+VVRKP   Q C+VTA GR N RE T L+  GCH  GDP+Y
Sbjct: 423 SGTVDFIFGDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAY 482

Query: 487 LPFKKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPG 546
           +P K VNKAYLGRPWK++SRTIIM + IDD+I P GWLPW GDFAL TL+YAE  N GPG
Sbjct: 483 IPMKSVNKAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPG 542

Query: 547 ADQSHRVKWRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSS 577
           ++Q+ RVKW GI+ +TPQ A  YT  RF+ GD WIP   VPY++
Sbjct: 543 SNQAQRVKWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

BLAST of Csor.00g259820.m01 vs. ExPASy Swiss-Prot
Match: Q42920 (Pectinesterase/pectinesterase inhibitor OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 4.5e-130
Identity = 228/450 (50.67%), Postives = 303/450 (67.33%), Query Frame = 0

Query: 138 LQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSAALTYEQTCLDGFENTTG 197
           ++ C E+L+YA+D +H S   +  F   K  E+  DLK+WL+  L+++QTCLDGF NTT 
Sbjct: 1   MEICNEVLDYAVDGIHKSVGTLDQFDFHKLSEYAFDLKVWLTGTLSHQQTCLDGFANTTT 60

Query: 198 DSGERMKEFLKSAQEMTTNGLGIVSELSKIL-GTLHAPAATGRRLLDTD-MPLWVSDGKR 257
            +GE M + LK++ E+++N + ++  +S+IL G   +  +  RRLL  D +P WV+DG R
Sbjct: 61  KAGETMTKVLKTSMELSSNAIDMMDAVSRILKGFDTSQYSVSRRLLSDDGIPSWVNDGHR 120

Query: 258 RLMQMGTGAIKPDLVVAQDGSGKYKTINEALVHVPMKSNKTFVIYVKAGIYKEIVVIPKH 317
           RL  +  G ++P+ VVAQDGSG++KT+ +AL  VP K+   FVI+VKAG+YKE V + K 
Sbjct: 121 RL--LAGGNVQPNAVVAQDGSGQFKTLTDALKTVPPKNAVPFVIHVKAGVYKETVNVAKE 180

Query: 318 MTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGANFFVRDMGFVNTAGPTKHQAV 377
           M ++T+ GDGPTK+  TG+LN+ DGI T+ TATF   GANF  +D+GF NTAG  KHQAV
Sbjct: 181 MNYVTVIGDGPTKTKFTGSLNYADGINTYNTATFGVNGANFMAKDIGFENTAGTGKHQAV 240

Query: 378 ALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGTIDFVFGNSAAIFQNCKLVVR 437
           ALRV +D+AI YNCQ+DG+QDTLY  + RQFYRDC ISGTIDFVFG    +FQNCKLV R
Sbjct: 241 ALRVTADQAIFYNCQMDGFQDTLYVQSQRQFYRDCSISGTIDFVFGERFGVFQNCKLVCR 300

Query: 438 KPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSYLPFKKVNKAYLGRPWKQYSRTII 497
            P   QQC+VTA GR  +   +AL+FQ  HF G+P+ L       +YLGRPWKQYS+ +I
Sbjct: 301 LPAKGQQCLVTAGGREKQNSASALVFQSSHFTGEPA-LTSVTPKVSYLGRPWKQYSKVVI 360

Query: 498 MGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQSHRVKWRGIRHITPQHAAAY 557
           M S ID +  PEG++PWMG     T  Y E  N+GPGAD + RVKW G++ +T   AA Y
Sbjct: 361 MDSTIDAIFVPEGYMPWMGSAFKETCTYYEYNNKGPGADTNLRVKWHGVKVLTSNVAAEY 420

Query: 558 TARRFID------GDAWIPVRGVPYSSGMM 580
              +F +       D WI   GVPYS G M
Sbjct: 421 YPGKFFEIVNATARDTWIVKSGVPYSLGPM 447

BLAST of Csor.00g259820.m01 vs. NCBI nr
Match: KAG6575080.1 (putative pectinesterase/pectinesterase inhibitor 21, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1169 bits (3023), Expect = 0.0
Identity = 579/579 (100.00%), Postives = 579/579 (100.00%), Query Frame = 0

Query: 1   MSNGFDAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHG 60
           MSNGFDAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHG
Sbjct: 1   MSNGFDAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHG 60

Query: 61  DDGEKVSSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIR 120
           DDGEKVSSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIR
Sbjct: 61  DDGEKVSSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIR 120

Query: 121 NSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSA 180
           NSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSA
Sbjct: 121 NSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSA 180

Query: 181 ALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLHAPAATGRR 240
           ALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLHAPAATGRR
Sbjct: 181 ALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLHAPAATGRR 240

Query: 241 LLDTDMPLWVSDGKRRLMQMGTGAIKPDLVVAQDGSGKYKTINEALVHVPMKSNKTFVIY 300
           LLDTDMPLWVSDGKRRLMQMGTGAIKPDLVVAQDGSGKYKTINEALVHVPMKSNKTFVIY
Sbjct: 241 LLDTDMPLWVSDGKRRLMQMGTGAIKPDLVVAQDGSGKYKTINEALVHVPMKSNKTFVIY 300

Query: 301 VKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGANFFVRD 360
           VKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGANFFVRD
Sbjct: 301 VKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGANFFVRD 360

Query: 361 MGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGTIDFVF 420
           MGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGTIDFVF
Sbjct: 361 MGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGTIDFVF 420

Query: 421 GNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSYLPFKKVNK 480
           GNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSYLPFKKVNK
Sbjct: 421 GNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSYLPFKKVNK 480

Query: 481 AYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQSHRVK 540
           AYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQSHRVK
Sbjct: 481 AYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQSHRVK 540

Query: 541 WRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM 579
           WRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM
Sbjct: 541 WRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM 579

BLAST of Csor.00g259820.m01 vs. NCBI nr
Match: XP_023549205.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1160 bits (3002), Expect = 0.0
Identity = 575/579 (99.31%), Postives = 576/579 (99.48%), Query Frame = 0

Query: 1   MSNGFDAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHG 60
           MSNGFDAE DKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHG
Sbjct: 1   MSNGFDAEGDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHG 60

Query: 61  DDGEKVSSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIR 120
           DDGEKVSSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIR
Sbjct: 61  DDGEKVSSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIR 120

Query: 121 NSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSA 180
           NSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSA
Sbjct: 121 NSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSA 180

Query: 181 ALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLHAPAATGRR 240
           ALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELS ILGTLHAPAATGRR
Sbjct: 181 ALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSNILGTLHAPAATGRR 240

Query: 241 LLDTDMPLWVSDGKRRLMQMGTGAIKPDLVVAQDGSGKYKTINEALVHVPMKSNKTFVIY 300
           LLDTDMPLWVSDGKRRLMQMGTGAIKPDLVVAQDGSGKYKTINEALV VPMKSNKTFVIY
Sbjct: 241 LLDTDMPLWVSDGKRRLMQMGTGAIKPDLVVAQDGSGKYKTINEALVDVPMKSNKTFVIY 300

Query: 301 VKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGANFFVRD 360
           VKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGANFFVRD
Sbjct: 301 VKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGANFFVRD 360

Query: 361 MGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGTIDFVF 420
           MGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGTIDFVF
Sbjct: 361 MGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGTIDFVF 420

Query: 421 GNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSYLPFKKVNK 480
           GNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDP+YLPFKKVNK
Sbjct: 421 GNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPAYLPFKKVNK 480

Query: 481 AYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQSHRVK 540
           AYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQSHRVK
Sbjct: 481 AYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQSHRVK 540

Query: 541 WRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM 579
           WRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM
Sbjct: 541 WRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM 579

BLAST of Csor.00g259820.m01 vs. NCBI nr
Match: XP_022959432.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita moschata])

HSP 1 Score: 1159 bits (2999), Expect = 0.0
Identity = 574/579 (99.14%), Postives = 577/579 (99.65%), Query Frame = 0

Query: 1   MSNGFDAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHG 60
           MSNGFDAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHG
Sbjct: 1   MSNGFDAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHG 60

Query: 61  DDGEKVSSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIR 120
           DD EK+SSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIR
Sbjct: 61  DDAEKISSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIR 120

Query: 121 NSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSA 180
           NSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSA
Sbjct: 121 NSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSA 180

Query: 181 ALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLHAPAATGRR 240
           ALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTL APAATGRR
Sbjct: 181 ALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLDAPAATGRR 240

Query: 241 LLDTDMPLWVSDGKRRLMQMGTGAIKPDLVVAQDGSGKYKTINEALVHVPMKSNKTFVIY 300
           LLDTD+PLWVSDGKRRLMQ+GTGAIKPDLVVAQDGSGKYKTINEALVHVPMKSNKTFVIY
Sbjct: 241 LLDTDIPLWVSDGKRRLMQVGTGAIKPDLVVAQDGSGKYKTINEALVHVPMKSNKTFVIY 300

Query: 301 VKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGANFFVRD 360
           VKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGANFFVRD
Sbjct: 301 VKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGANFFVRD 360

Query: 361 MGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGTIDFVF 420
           MGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGTIDFVF
Sbjct: 361 MGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGTIDFVF 420

Query: 421 GNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSYLPFKKVNK 480
           GNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSYLPFKKVNK
Sbjct: 421 GNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSYLPFKKVNK 480

Query: 481 AYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQSHRVK 540
           AYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQSHRVK
Sbjct: 481 AYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQSHRVK 540

Query: 541 WRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM 579
           WRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM
Sbjct: 541 WRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM 579

BLAST of Csor.00g259820.m01 vs. NCBI nr
Match: XP_023006255.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita maxima])

HSP 1 Score: 1126 bits (2913), Expect = 0.0
Identity = 562/600 (93.67%), Postives = 570/600 (95.00%), Query Frame = 0

Query: 1   MSNGFDAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGG-------- 60
           MSNGFDAE DKKKKKIAVISVSSLILVAMVVAVTVGVNNAP EDLDDGGGGG        
Sbjct: 1   MSNGFDAEGDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPHEDLDDGGGGGDLHDHGDD 60

Query: 61  -------------GGDAHDHGDDGEKVSSSTKAIKTICQPTDYKETCEESLMVEAGNTTD 120
                        GGDAHDHGDDGEK+SSSTKAIKTICQPTDYKETCEESLMVEAGNTTD
Sbjct: 61  GGDVHDHGDYGGGGGDAHDHGDDGEKISSSTKAIKTICQPTDYKETCEESLMVEAGNTTD 120

Query: 121 PKELVKAGFKIAVEKLNAAIRNSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRI 180
            KELVKAGFKIAVEKLNAAIRNSTTLQELA+DPRANQALQNCRELLEYAIDDLHDSYHRI
Sbjct: 121 LKELVKAGFKIAVEKLNAAIRNSTTLQELAKDPRANQALQNCRELLEYAIDDLHDSYHRI 180

Query: 181 GTFHVSKFEEFVNDLKIWLSAALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLG 240
           GTFHVSKFEEFVNDLKIWLSAALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLG
Sbjct: 181 GTFHVSKFEEFVNDLKIWLSAALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLG 240

Query: 241 IVSELSKILGTLHAPAATGRRLLDTDMPLWVSDGKRRLMQMGTGAIKPDLVVAQDGSGKY 300
           IVSELS ILGTLH PAATGRRLLDTDMPLWVSDGKRRLMQ+ TGAIKPDLVVAQDGSGKY
Sbjct: 241 IVSELSNILGTLHEPAATGRRLLDTDMPLWVSDGKRRLMQLETGAIKPDLVVAQDGSGKY 300

Query: 301 KTINEALVHVPMKSNKTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVD 360
           KTINEALV VPMKSNKTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVD
Sbjct: 301 KTINEALVDVPMKSNKTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVD 360

Query: 361 GIQTFKTATFAAIGANFFVRDMGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLY 420
           GIQTFKTATFAAIGANFFVRDMGFVN+AGPTKHQAVALR+QSDRAIIYNCQIDG+QDTLY
Sbjct: 361 GIQTFKTATFAAIGANFFVRDMGFVNSAGPTKHQAVALRIQSDRAIIYNCQIDGFQDTLY 420

Query: 421 AHANRQFYRDCIISGTIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTAL 480
           AHANRQFYRDC ISGTIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTAL
Sbjct: 421 AHANRQFYRDCTISGTIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTAL 480

Query: 481 IFQGCHFMGDPSYLPFKKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALN 540
           IFQGCHFMGDP+YLPFKKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDF LN
Sbjct: 481 IFQGCHFMGDPAYLPFKKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFGLN 540

Query: 541 TLFYAEVQNRGPGADQSHRVKWRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM 579
           TLFYAEVQNRGPGADQSHRVKWRGIRHITPQHAA+YTARRFIDGDAWIPVRGVPYSSGMM
Sbjct: 541 TLFYAEVQNRGPGADQSHRVKWRGIRHITPQHAASYTARRFIDGDAWIPVRGVPYSSGMM 600

BLAST of Csor.00g259820.m01 vs. NCBI nr
Match: XP_008458409.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Cucumis melo])

HSP 1 Score: 964 bits (2493), Expect = 0.0
Identity = 473/587 (80.58%), Postives = 518/587 (88.25%), Query Frame = 0

Query: 1   MSNGF-DAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDH 60
           MSNGF +AE DKKKKKIA+ISVSSLILVAMVVAVTVG+NNAP E+            H  
Sbjct: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGINNAPYEE------------HGE 60

Query: 61  GDDGEK----VSSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKL 120
           GD G K    +SSSTKAI+ ICQPTDYKETCE+SLM EAGNTTDPKELVK GFKIA   L
Sbjct: 61  GDQGSKTTPEISSSTKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSL 120

Query: 121 NAAIRNSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLK 180
           N AIRNSTTLQEL++DPR NQALQNCRELLEYAIDDL++S+ RIGTF +SK +EFV DLK
Sbjct: 121 NEAIRNSTTLQELSKDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLK 180

Query: 181 IWLSAALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLHAPA 240
           IWLS ALTYEQTCLDGFENTTGD+G RM+EFLKSAQEMTTNGLGIV+ELS ILG+L  P 
Sbjct: 181 IWLSGALTYEQTCLDGFENTTGDAGTRMQEFLKSAQEMTTNGLGIVNELSTILGSLQIPG 240

Query: 241 ATGRRLLDTD---MPLWVSDGKRRLMQMGTGAIKPDLVVAQDGSGKYKTINEALVHVPMK 300
            TGRRLL+ D   MP W+SDGKRRLMQ G   +KPDL+VAQDGSGKYKTIN AL  VP+K
Sbjct: 241 MTGRRLLEDDDHEMPSWISDGKRRLMQAGAAGMKPDLIVAQDGSGKYKTINAALADVPLK 300

Query: 301 SNKTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAI 360
           SNKTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTK+VVTG+LNF+DGIQTFKTATF+AI
Sbjct: 301 SNKTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAI 360

Query: 361 GANFFVRDMGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCII 420
           GANF+ RDMGF NTAG  KHQAVALRVQSDR+I YNCQIDGYQDTLYAHA+RQFYRDC I
Sbjct: 361 GANFYARDMGFENTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTI 420

Query: 421 SGTIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSY 480
           SGTIDFVFGN+A  FQNCKLVVRKP+DNQQCIVTAHGR+NR+EPTALIFQGCHFMGDP+Y
Sbjct: 421 SGTIDFVFGNAATNFQNCKLVVRKPMDNQQCIVTAHGRMNRKEPTALIFQGCHFMGDPAY 480

Query: 481 LPFKKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPG 540
           LPFK +NKAYLGRPWKQYSRTII+GSQIDD+IQPEGWLPWMGDF LNTLFYAEVQN+GPG
Sbjct: 481 LPFKSINKAYLGRPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGPG 540

Query: 541 ADQSHRVKWRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM 579
           A ++ RVKWRGI+HITPQHAA +T RRFIDGDAWIP +G+PYSSGMM
Sbjct: 541 AVETKRVKWRGIKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 575

BLAST of Csor.00g259820.m01 vs. ExPASy TrEMBL
Match: A0A6J1H4H8 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111460411 PE=3 SV=1)

HSP 1 Score: 1159 bits (2999), Expect = 0.0
Identity = 574/579 (99.14%), Postives = 577/579 (99.65%), Query Frame = 0

Query: 1   MSNGFDAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHG 60
           MSNGFDAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHG
Sbjct: 1   MSNGFDAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHG 60

Query: 61  DDGEKVSSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIR 120
           DD EK+SSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIR
Sbjct: 61  DDAEKISSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIR 120

Query: 121 NSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSA 180
           NSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSA
Sbjct: 121 NSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSA 180

Query: 181 ALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLHAPAATGRR 240
           ALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTL APAATGRR
Sbjct: 181 ALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLDAPAATGRR 240

Query: 241 LLDTDMPLWVSDGKRRLMQMGTGAIKPDLVVAQDGSGKYKTINEALVHVPMKSNKTFVIY 300
           LLDTD+PLWVSDGKRRLMQ+GTGAIKPDLVVAQDGSGKYKTINEALVHVPMKSNKTFVIY
Sbjct: 241 LLDTDIPLWVSDGKRRLMQVGTGAIKPDLVVAQDGSGKYKTINEALVHVPMKSNKTFVIY 300

Query: 301 VKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGANFFVRD 360
           VKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGANFFVRD
Sbjct: 301 VKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGANFFVRD 360

Query: 361 MGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGTIDFVF 420
           MGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGTIDFVF
Sbjct: 361 MGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGTIDFVF 420

Query: 421 GNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSYLPFKKVNK 480
           GNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSYLPFKKVNK
Sbjct: 421 GNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSYLPFKKVNK 480

Query: 481 AYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQSHRVK 540
           AYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQSHRVK
Sbjct: 481 AYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQSHRVK 540

Query: 541 WRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM 579
           WRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM
Sbjct: 541 WRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM 579

BLAST of Csor.00g259820.m01 vs. ExPASy TrEMBL
Match: A0A6J1KZN0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499042 PE=3 SV=1)

HSP 1 Score: 1126 bits (2913), Expect = 0.0
Identity = 562/600 (93.67%), Postives = 570/600 (95.00%), Query Frame = 0

Query: 1   MSNGFDAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGG-------- 60
           MSNGFDAE DKKKKKIAVISVSSLILVAMVVAVTVGVNNAP EDLDDGGGGG        
Sbjct: 1   MSNGFDAEGDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPHEDLDDGGGGGDLHDHGDD 60

Query: 61  -------------GGDAHDHGDDGEKVSSSTKAIKTICQPTDYKETCEESLMVEAGNTTD 120
                        GGDAHDHGDDGEK+SSSTKAIKTICQPTDYKETCEESLMVEAGNTTD
Sbjct: 61  GGDVHDHGDYGGGGGDAHDHGDDGEKISSSTKAIKTICQPTDYKETCEESLMVEAGNTTD 120

Query: 121 PKELVKAGFKIAVEKLNAAIRNSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRI 180
            KELVKAGFKIAVEKLNAAIRNSTTLQELA+DPRANQALQNCRELLEYAIDDLHDSYHRI
Sbjct: 121 LKELVKAGFKIAVEKLNAAIRNSTTLQELAKDPRANQALQNCRELLEYAIDDLHDSYHRI 180

Query: 181 GTFHVSKFEEFVNDLKIWLSAALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLG 240
           GTFHVSKFEEFVNDLKIWLSAALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLG
Sbjct: 181 GTFHVSKFEEFVNDLKIWLSAALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLG 240

Query: 241 IVSELSKILGTLHAPAATGRRLLDTDMPLWVSDGKRRLMQMGTGAIKPDLVVAQDGSGKY 300
           IVSELS ILGTLH PAATGRRLLDTDMPLWVSDGKRRLMQ+ TGAIKPDLVVAQDGSGKY
Sbjct: 241 IVSELSNILGTLHEPAATGRRLLDTDMPLWVSDGKRRLMQLETGAIKPDLVVAQDGSGKY 300

Query: 301 KTINEALVHVPMKSNKTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVD 360
           KTINEALV VPMKSNKTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVD
Sbjct: 301 KTINEALVDVPMKSNKTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVD 360

Query: 361 GIQTFKTATFAAIGANFFVRDMGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLY 420
           GIQTFKTATFAAIGANFFVRDMGFVN+AGPTKHQAVALR+QSDRAIIYNCQIDG+QDTLY
Sbjct: 361 GIQTFKTATFAAIGANFFVRDMGFVNSAGPTKHQAVALRIQSDRAIIYNCQIDGFQDTLY 420

Query: 421 AHANRQFYRDCIISGTIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTAL 480
           AHANRQFYRDC ISGTIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTAL
Sbjct: 421 AHANRQFYRDCTISGTIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTAL 480

Query: 481 IFQGCHFMGDPSYLPFKKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALN 540
           IFQGCHFMGDP+YLPFKKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDF LN
Sbjct: 481 IFQGCHFMGDPAYLPFKKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFGLN 540

Query: 541 TLFYAEVQNRGPGADQSHRVKWRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM 579
           TLFYAEVQNRGPGADQSHRVKWRGIRHITPQHAA+YTARRFIDGDAWIPVRGVPYSSGMM
Sbjct: 541 TLFYAEVQNRGPGADQSHRVKWRGIRHITPQHAASYTARRFIDGDAWIPVRGVPYSSGMM 600

BLAST of Csor.00g259820.m01 vs. ExPASy TrEMBL
Match: A0A1S3C7B6 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497833 PE=3 SV=1)

HSP 1 Score: 964 bits (2493), Expect = 0.0
Identity = 473/587 (80.58%), Postives = 518/587 (88.25%), Query Frame = 0

Query: 1   MSNGF-DAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDH 60
           MSNGF +AE DKKKKKIA+ISVSSLILVAMVVAVTVG+NNAP E+            H  
Sbjct: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGINNAPYEE------------HGE 60

Query: 61  GDDGEK----VSSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKL 120
           GD G K    +SSSTKAI+ ICQPTDYKETCE+SLM EAGNTTDPKELVK GFKIA   L
Sbjct: 61  GDQGSKTTPEISSSTKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSL 120

Query: 121 NAAIRNSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLK 180
           N AIRNSTTLQEL++DPR NQALQNCRELLEYAIDDL++S+ RIGTF +SK +EFV DLK
Sbjct: 121 NEAIRNSTTLQELSKDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLK 180

Query: 181 IWLSAALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLHAPA 240
           IWLS ALTYEQTCLDGFENTTGD+G RM+EFLKSAQEMTTNGLGIV+ELS ILG+L  P 
Sbjct: 181 IWLSGALTYEQTCLDGFENTTGDAGTRMQEFLKSAQEMTTNGLGIVNELSTILGSLQIPG 240

Query: 241 ATGRRLLDTD---MPLWVSDGKRRLMQMGTGAIKPDLVVAQDGSGKYKTINEALVHVPMK 300
            TGRRLL+ D   MP W+SDGKRRLMQ G   +KPDL+VAQDGSGKYKTIN AL  VP+K
Sbjct: 241 MTGRRLLEDDDHEMPSWISDGKRRLMQAGAAGMKPDLIVAQDGSGKYKTINAALADVPLK 300

Query: 301 SNKTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAI 360
           SNKTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTK+VVTG+LNF+DGIQTFKTATF+AI
Sbjct: 301 SNKTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAI 360

Query: 361 GANFFVRDMGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCII 420
           GANF+ RDMGF NTAG  KHQAVALRVQSDR+I YNCQIDGYQDTLYAHA+RQFYRDC I
Sbjct: 361 GANFYARDMGFENTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTI 420

Query: 421 SGTIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSY 480
           SGTIDFVFGN+A  FQNCKLVVRKP+DNQQCIVTAHGR+NR+EPTALIFQGCHFMGDP+Y
Sbjct: 421 SGTIDFVFGNAATNFQNCKLVVRKPMDNQQCIVTAHGRMNRKEPTALIFQGCHFMGDPAY 480

Query: 481 LPFKKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPG 540
           LPFK +NKAYLGRPWKQYSRTII+GSQIDD+IQPEGWLPWMGDF LNTLFYAEVQN+GPG
Sbjct: 481 LPFKSINKAYLGRPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGPG 540

Query: 541 ADQSHRVKWRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM 579
           A ++ RVKWRGI+HITPQHAA +T RRFIDGDAWIP +G+PYSSGMM
Sbjct: 541 AVETKRVKWRGIKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 575

BLAST of Csor.00g259820.m01 vs. ExPASy TrEMBL
Match: A0A0A0KD24 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G318700 PE=3 SV=1)

HSP 1 Score: 947 bits (2447), Expect = 0.0
Identity = 467/589 (79.29%), Postives = 514/589 (87.27%), Query Frame = 0

Query: 1   MSNGF-DAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDH 60
           MSNGF +AE DKKKKKIA+ISVSSLILVAMVVAVTVGVNNAP                +H
Sbjct: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPH-------------GEEH 60

Query: 61  GDDGE------KVSSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVE 120
           GDD        +++SSTKAI+ ICQPTDYKETCE+SL  EAGNTTDPKELVK GFKIA  
Sbjct: 61  GDDNHGSETTPEITSSTKAIQAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATR 120

Query: 121 KLNAAIRNSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVND 180
            LN AI+NSTTL+ELA+DPR NQALQNCRELLEYAIDDL+ S+ RIGTF +SK ++FV D
Sbjct: 121 SLNEAIKNSTTLKELAKDPRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVAD 180

Query: 181 LKIWLSAALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLHA 240
           LKIWLS ALTYEQTCLDGFENTTGD+G RM+EFLKSAQ+MTTNGLGIV+ELS ILG+L  
Sbjct: 181 LKIWLSGALTYEQTCLDGFENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQL 240

Query: 241 PAATGRRLLDTD---MPLWVSDGKRRLMQMGTGAIKPDLVVAQDGSGKYKTINEALVHVP 300
           P  +GRRLL+ D   MP WVSDGKRRLMQ G  A+KPDLVVAQDGSGKYKTIN AL  VP
Sbjct: 241 PGMSGRRLLEDDDREMPSWVSDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVP 300

Query: 301 MKSNKTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFA 360
           +KSNKTFVI+VKAGIYKEIVVIPKHMTHLTMYGDGPTK+VVTG+LNF+DGIQTFKTATF+
Sbjct: 301 LKSNKTFVIHVKAGIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFS 360

Query: 361 AIGANFFVRDMGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDC 420
           AIGANF+ RDMGF NTAG  KHQAVALRVQSDR+I +NCQIDGYQDTLYAHA+RQFYRDC
Sbjct: 361 AIGANFYARDMGFENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDC 420

Query: 421 IISGTIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDP 480
            ISGTIDFVFGN+A  FQNCKLVVRKPLDNQQCIVTAHGRLNR+EPTALIFQ CHFMGDP
Sbjct: 421 TISGTIDFVFGNAATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDP 480

Query: 481 SYLPFKKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRG 540
           +YLPFK +NKAYLGRPWK+YSRTII+GS IDD+IQPEGWLPWMGDF LNTLFYAEVQN+G
Sbjct: 481 AYLPFKAINKAYLGRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKG 540

Query: 541 PGADQSHRVKWRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM 579
            GAD+S RVKWRGI+HITPQHAA +T RRFIDGDAWIP +G+PYSSGMM
Sbjct: 541 AGADESKRVKWRGIKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 576

BLAST of Csor.00g259820.m01 vs. ExPASy TrEMBL
Match: A0A6J1ER80 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111436828 PE=3 SV=1)

HSP 1 Score: 873 bits (2256), Expect = 1.95e-315
Identity = 432/580 (74.48%), Postives = 495/580 (85.34%), Query Frame = 0

Query: 1   MSNGFDAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHG 60
           MSNGFDAE D +KKK+A++ VSSL LVAMVVAVTVGVNN+  +        G G  + HG
Sbjct: 1   MSNGFDAEGDNEKKKMALVGVSSLFLVAMVVAVTVGVNNSSHK--------GEGLGNGHG 60

Query: 61  DDGEKVSSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIR 120
           D   ++SSSTKAI+TIC+PTDYKETCEESLM E  NTTDP+ELVK GFK+ +++LN+AIR
Sbjct: 61  DGAGEISSSTKAIQTICRPTDYKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIR 120

Query: 121 NSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSA 180
           NSTTLQ LA++PRANQALQNCRELLEYAIDD+ DS++RIGTF ++K ++FV DL +WLS 
Sbjct: 121 NSTTLQALAKEPRANQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSG 180

Query: 181 ALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLHAPAATGRR 240
           ALTYEQTCLDGFENTTGD+GE+MK+FLKSAQEMT+NGL IV+EL  +LG+L    A GRR
Sbjct: 181 ALTYEQTCLDGFENTTGDAGEQMKKFLKSAQEMTSNGLAIVTELKNMLGSLQGSKA-GRR 240

Query: 241 LLDTD-MPLWVSDGKRRLMQMGTGAIKPDLVVAQDGSGKYKTINEALVHVPMKSNKTFVI 300
           LL  D +P WVSD KRRLMQ+ T AI P++VVAQDGSGKYKTINEALV V  KSN TFVI
Sbjct: 241 LLAGDEVPSWVSDEKRRLMQVPTAAITPNVVVAQDGSGKYKTINEALVEVLKKSNITFVI 300

Query: 301 YVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGANFFVR 360
           YVKAGIYKE V+IPK MTH+TMYGDGPTK+VV+G+LNFVDGIQTFKTATFA +GANFF R
Sbjct: 301 YVKAGIYKETVLIPKAMTHVTMYGDGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCR 360

Query: 361 DMGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGTIDFV 420
           DMGF NTAG TKHQAVALRVQSDR+I +NC +DG+QDTLYA  +RQFYRDC +SGTIDFV
Sbjct: 361 DMGFENTAGATKHQAVALRVQSDRSIFFNCHMDGHQDTLYAQTHRQFYRDCKVSGTIDFV 420

Query: 421 FGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSYLPFKKVN 480
           FGNSAA+FQNC LVVRKPL NQQCIVTA GRL  REPT L+FQ CHFMGDP+YLPFK VN
Sbjct: 421 FGNSAAVFQNCNLVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVN 480

Query: 481 KAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQSHRV 540
           KAYLGRPWKQ+SRTIIMGS+IDDLIQPEGWLPWMGDF LNTLFYAE QN+GPGAD ++RV
Sbjct: 481 KAYLGRPWKQFSRTIIMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRV 540

Query: 541 KWRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM 579
           KW+GI+ +TPQ AA +T + FIDGD WI   GVPYS GMM
Sbjct: 541 KWQGIKTLTPQQAADFTPKIFIDGDDWILNTGVPYSPGMM 571

BLAST of Csor.00g259820.m01 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 637.9 bits (1644), Expect = 8.0e-183
Identity = 298/578 (51.56%), Postives = 410/578 (70.93%), Query Frame = 0

Query: 1   MSNGFDAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHG 60
           MS G+D E  K+KK+  +IS+SS++L++MVVAVT+GV+    ++  D             
Sbjct: 1   MSYGYDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAGD------------- 60

Query: 61  DDGEKVSSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIR 120
              E++++S KAIK +C PTDYKETCE++L  +A +T+DP ELVK  F   +++++   +
Sbjct: 61  ---EEITTSVKAIKDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAK 120

Query: 121 NSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSA 180
            S T+ EL +DPRA  AL  C+EL++YAI +L  S+  +G F   K +E +  L+IWLSA
Sbjct: 121 KSQTMIELQKDPRAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSA 180

Query: 181 ALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLHAPAATGRR 240
            +++EQTCLDGF+ T G++GE +K+ LK+A ++T NGL +V+E+S  LG +  P    RR
Sbjct: 181 TISHEQTCLDGFQGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRR 240

Query: 241 LLDTDMPLWVSDGKRRLMQMGTGAIKPDLVVAQDGSGKYKTINEALVHVPMKSNKTFVIY 300
           LL  + P W+    RRL+      +KPD+VVAQDGSG+YKTINEAL  VP K N TFV++
Sbjct: 241 LLSQEFPSWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVH 300

Query: 301 VKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGANFFVRD 360
           +K GIYKE V + + MTHL   GDGP K+V++G+ ++ DGI T+KTAT A +G +F  ++
Sbjct: 301 IKEGIYKEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKN 360

Query: 361 MGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGTIDFVF 420
           + F NTAG  KHQAVA+RV +D +I YNC+ DGYQDTLYAH++RQFYRDC ISGTIDF+F
Sbjct: 361 IAFENTAGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLF 420

Query: 421 GNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSYLPFKKVNK 480
           G++AA+FQNC L+VRKPL NQ C +TAHGR + RE T  + QGC  +G+P YL  K+ +K
Sbjct: 421 GDAAAVFQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSK 480

Query: 481 AYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQSHRVK 540
            YLGRPWK+YSRTIIM + I D + PEGW PW+G+F LNTLFY+EVQN GPGA  + RV 
Sbjct: 481 TYLGRPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVT 540

Query: 541 WRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGM 579
           W GI+ ++ +    +T  ++I GDAWIP +GVPY  G+
Sbjct: 541 WPGIKKLSDEEILKFTPAQYIQGDAWIPGKGVPYILGL 562

BLAST of Csor.00g259820.m01 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 635.2 bits (1637), Expect = 5.2e-182
Identity = 300/581 (51.64%), Postives = 418/581 (71.94%), Query Frame = 0

Query: 1   MSNGFDAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHG 60
           MS G+D ES K+K++  VI++SS++L++MVVAVTVGV+      L+   G   G A    
Sbjct: 1   MSYGYDDES-KRKRRYIVITISSVLLISMVVAVTVGVS------LNKHDGDSKGKA---- 60

Query: 61  DDGEKVSSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIR 120
               +V++S KA+K +C PTDY++TCE++L+    NTTDP ELVK  F + ++++  A +
Sbjct: 61  ----EVNASVKAVKDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAK 120

Query: 121 NSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSA 180
            S T+ EL +D R   AL  C+EL++YA+D+L +S+  +G F     +E + +L+IWLSA
Sbjct: 121 KSQTIMELQKDSRTRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSA 180

Query: 181 ALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLHAPAATGRR 240
           A+++E+TCL+GF+ T G++GE MK+ LK+A E+T NGL I+SE+S  +G +  P    RR
Sbjct: 181 AISHEETCLEGFQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRR 240

Query: 241 LLDTDMPLWVSDGKRRLMQMGT--GAIKPDLVVAQDGSGKYKTINEALVHVPMKSNKTFV 300
           LL    P WV    R+L+Q       +KPD+VVAQDGSG+YKTINEAL  VP K N TFV
Sbjct: 241 LLAEGFPSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFV 300

Query: 301 IYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGANFFV 360
           +++KAG+YKE V + K M+HL   GDGP K++++GN N+ DGI T++TAT A +G  F  
Sbjct: 301 VHIKAGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIA 360

Query: 361 RDMGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGTIDF 420
           +++GF NTAG  KHQAVA+RVQSD +I +NC+ DGYQDTLY H++RQF+RDC ISGTIDF
Sbjct: 361 KNIGFENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDF 420

Query: 421 VFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSYLPFKKV 480
           +FG++AA+FQNC L+VRKPL NQ C +TAHGR + RE T  +FQGC   G+P YL  K+ 
Sbjct: 421 LFGDAAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKET 480

Query: 481 NKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQSHR 540
           +KAYLGRPWK+YSRTIIM + I D +QP+GW PW+GDF L TLFY+EVQN GPG+  ++R
Sbjct: 481 SKAYLGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANR 540

Query: 541 VKWRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM 580
           V W GI+ ++ +    +T  ++I GD WIP +GVPY++G++
Sbjct: 541 VTWAGIKTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLL 566

BLAST of Csor.00g259820.m01 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 565.1 bits (1455), Expect = 6.6e-161
Identity = 268/582 (46.05%), Postives = 382/582 (65.64%), Query Frame = 0

Query: 4   GFDAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHGDDG 63
           G D E  KKK  IA +  + L+L+ + V +T   N +  E +                  
Sbjct: 2   GVDGELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVP---------------- 61

Query: 64  EKVSSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIRNST 123
            ++ ++T A++ +C PTDYKETC  SLM  + ++T P +L+K GF + +  +  +I+ ++
Sbjct: 62  VQIKTATTAVEAVCAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKAS 121

Query: 124 T--LQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSAA 183
                + A D     AL+ C +L+  A DDL         F + + E+FV DL++WLS +
Sbjct: 122 VELTAKAANDKDTKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGS 181

Query: 184 LTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLHAPAATG--- 243
           + Y+QTC+D FE T     + M++  K+++E+T+NGL +++ +S +LG  +    TG   
Sbjct: 182 IAYQQTCMDTFEETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLG 241

Query: 244 ---RRLLDTD--MPLWVSDGKRRLMQMGTGAIKPDLVVAQDGSGKYKTINEALVHVPMKS 303
              R+LL  +  +P WV    RRLM    G +K ++VVA DGSG+YKTINEAL  VP  +
Sbjct: 242 KYARKLLSAEDGIPSWVGPNTRRLMAT-KGGVKANVVVAHDGSGQYKTINEALNAVPKAN 301

Query: 304 NKTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDG-IQTFKTATFAAI 363
            K FVIY+K G+Y E V + K MTH+T  GDGPTK+ +TG+LN+  G ++T+ TAT A  
Sbjct: 302 QKPFVIYIKQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAIN 361

Query: 364 GANFFVRDMGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCII 423
           G NF  +++GF NTAGP  HQAVALRV +D A+ YNCQIDGYQDTLY H++RQF+RDC +
Sbjct: 362 GDNFTAKNIGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTV 421

Query: 424 SGTIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSY 483
           SGT+DF+FG+   + QNC +VVRKP+ +Q C++TA GR ++RE T L+ Q CH  G+P+Y
Sbjct: 422 SGTVDFIFGDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAY 481

Query: 484 LPFKKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPG 543
           +P K +NKAYLGRPWK++SRTIIMG+ IDD+I P GWLPW GDFALNTL+YAE +N GPG
Sbjct: 482 IPVKSINKAYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPG 541

Query: 544 ADQSHRVKWRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPY 575
           ++Q+ RVKW GI+ ++P+ A  +T  RF+ G+ WIP   VPY
Sbjct: 542 SNQAQRVKWPGIKKLSPKQALRFTPARFLRGNLWIPPNRVPY 566

BLAST of Csor.00g259820.m01 vs. TAIR 10
Match: AT3G06830.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 530.0 bits (1364), Expect = 2.4e-150
Identity = 267/584 (45.72%), Postives = 376/584 (64.38%), Query Frame = 0

Query: 7   AESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHGDDGEKV 66
           ++ DKKKK I   SVS   LV MVV+V V  +                  H   DD   +
Sbjct: 3   SDGDKKKKFIVAGSVSG-FLVIMVVSVAVVTSK-----------------HSPKDDENHI 62

Query: 67  SSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIRNST--T 126
             +TKA++ +C PTD+K+TC  SLM  + ++ DP +L+K GFK+ ++ +N ++  ++   
Sbjct: 63  RKTTKAVQAVCAPTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDI 122

Query: 127 LQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSAALTY 186
             +  ++P A  A + C +L+  AIDDL         F V + E FV DL++WLS ++ +
Sbjct: 123 KAKADKNPEAKGAFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAF 182

Query: 187 EQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLHAPAATG------ 246
           +QTC+D F     +  + M +  K+++E+++N L +V+ +S ++   +    TG      
Sbjct: 183 QQTCMDSFGEIKSNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYA 242

Query: 247 RRLLDTD--MPLWVSDGKRRLMQM---GTGAIKPDLVVAQDGSGKYKTINEALVHVPMKS 306
           R+LL T+  +P WV    RRLM     G G +K + VVAQDG+G++KTI +AL  VP  +
Sbjct: 243 RKLLSTEDSIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGN 302

Query: 307 NKTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNF-VDGIQTFKTATFAAI 366
              F+I++K GIYKE V + K M H+T  GDGP K+++TG+LNF +  ++TF TAT    
Sbjct: 303 KVPFIIHIKEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIE 362

Query: 367 GANFFVRDMGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCII 426
           G +F  +++G  NTAGP   QAVALRV +D A+ ++CQIDG+QDTLY H++RQFYRDC +
Sbjct: 363 GDHFTAKNIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTV 422

Query: 427 SGTIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSY 486
           SGT+DF+FG++  I QNCK+VVRKP   Q C+VTA GR N RE T L+  GCH  GDP+Y
Sbjct: 423 SGTVDFIFGDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAY 482

Query: 487 LPFKKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPG 546
           +P K VNKAYLGRPWK++SRTIIM + IDD+I P GWLPW GDFAL TL+YAE  N GPG
Sbjct: 483 IPMKSVNKAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPG 542

Query: 547 ADQSHRVKWRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSS 577
           ++Q+ RVKW GI+ +TPQ A  YT  RF+ GD WIP   VPY++
Sbjct: 543 SNQAQRVKWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

BLAST of Csor.00g259820.m01 vs. TAIR 10
Match: AT4G15980.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 446.4 bits (1147), Expect = 3.4e-125
Identity = 253/713 (35.48%), Postives = 368/713 (51.61%), Query Frame = 0

Query: 15  KIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHGDDGEKVSSSTKAIK 74
           K  ++ V++LI+ AMV+ V     N+P   +++           H +    ++ +T +I 
Sbjct: 3   KYVLLGVTALIM-AMVICVEANDGNSPSRKMEE----------VHRESKLMITKTTVSI- 62

Query: 75  TICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAI-RNSTTLQELAQ-DP 134
            IC  TDYK+ C  SL      + DP+ L+++ F +A+  + + I R    L+  A  D 
Sbjct: 63  -ICASTDYKQDCTTSLATV--RSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADM 122

Query: 135 RANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSAALTYEQTCLDGF 194
              +AL  CREL++ AIDDL  +  +   F  ++  +FV DL +WLS ++TY+QTC+DGF
Sbjct: 123 HTREALNTCRELMDDAIDDLRKTRDKFRGFLFTRLSDFVEDLCVWLSGSITYQQTCIDGF 182

Query: 195 ENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLHAP------------------ 254
           E    ++   M+  ++  Q +T+NGL I + L K+L     P                  
Sbjct: 183 EGIDSEAAVMMERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGS 242

Query: 255 ------------------------------------------------------------ 314
                                                                       
Sbjct: 243 SRDESVGSSQDSPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQME 302

Query: 315 ---------AATGRRLLDT----------------------------------------- 374
                    A +G + LD                                          
Sbjct: 303 SSEDTPKKSAFSGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKL 362

Query: 375 ------------------DMPLWVSDGKRRLM--QMGTGAIKPDLVVAQDGSGKYKTINE 434
                             + P WV+   RRL+  +     IK ++VVA+DGSGK KTI +
Sbjct: 363 NPLNKLDSLKDRHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQ 422

Query: 435 ALVHVPMKSNKTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFV-DGIQT 494
           AL  VPMK+ K FVI++K G+YKE V + K M H+   GDGPTK+V+TG++ F+ D + T
Sbjct: 423 ALAMVPMKNTKKFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGT 482

Query: 495 FKTATFAAIGANFFVRDMGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHAN 554
           ++TA+ A  G  F  +D+GF NTAG  +HQAVALRV +D A+ +NC ++GYQDTLY H +
Sbjct: 483 YRTASVAVNGDYFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTH 542

Query: 555 RQFYRDCIISGTIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQG 577
           RQFYR+C +SGTIDFVFG++ A+FQNC+ V+R+P+++QQCIVTA GR +RRE T ++   
Sbjct: 543 RQFYRNCRVSGTIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHN 602

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3E8Z81.1e-18151.56Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3... [more]
Q8GX867.3e-18151.64Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3... [more]
Q9FJ219.3e-16046.05Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3... [more]
Q8GXA13.3e-14945.72Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3... [more]
Q429204.5e-13050.67Pectinesterase/pectinesterase inhibitor OS=Medicago sativa OX=3879 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAG6575080.10.0100.00putative pectinesterase/pectinesterase inhibitor 21, partial [Cucurbita argyrosp... [more]
XP_023549205.10.099.31probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita pepo subsp. pepo][more]
XP_022959432.10.099.14probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita moschata][more]
XP_023006255.10.093.67probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita maxima][more]
XP_008458409.10.080.58PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A6J1H4H80.099.14Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111460411 PE=3 SV=1[more]
A0A6J1KZN00.093.67Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499042 PE=3 SV=1[more]
A0A1S3C7B60.080.58Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497833 PE=3 SV=1[more]
A0A0A0KD240.079.29Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G318700 PE=3 SV=1[more]
A0A6J1ER801.95e-31574.48Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111436828 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G27870.18.0e-18351.56Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G05610.15.2e-18251.64Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G49180.16.6e-16146.05Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G06830.12.4e-15045.72Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G15980.13.4e-12535.48Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 68..220
e-value: 5.6E-38
score: 142.2
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 69..223
e-value: 8.0E-25
score: 85.2
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 72..219
e-value: 2.3E-27
score: 96.2
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 268..564
e-value: 1.2E-137
score: 457.6
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 262..578
e-value: 1.8E-115
score: 387.7
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 67..226
e-value: 3.2E-33
score: 116.9
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 71..225
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 50..68
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 41..70
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 17..578
NoneNo IPR availablePANTHERPTHR31707:SF337PECTINESTERASEcoord: 17..578
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 75..226
e-value: 1.21396E-45
score: 156.444
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 412..421
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 293..312
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 264..577

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Csor.00g259820Csor.00g259820gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g259820.m01.CDS01Csor.00g259820.m01.CDS01CDS
Csor.00g259820.m01.CDS02Csor.00g259820.m01.CDS02CDS


The following stop_codon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g259820.m01.stop_codon01Csor.00g259820.m01.stop_codon01stop_codon


The following terminal feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g259820.m01.terminal01Csor.00g259820.m01.terminal01terminal


The following intron feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g259820.m01.intron01Csor.00g259820.m01.intron01intron


The following initial feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g259820.m01.initial01Csor.00g259820.m01.initial01initial


The following start_codon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g259820.m01.start_codon01Csor.00g259820.m01.start_codon01start_codon


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Csor.00g259820.m01Csor.00g259820.m01-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity