Csor.00g230880.m01 (mRNA) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g230880.m01
TypemRNA
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionRegulator of Vps4 activity in the MVB pathway protein, putative
LocationCsor_Chr08: 4373728 .. 4375528 (-)
Sequence length1269
RNA-Seq ExpressionCsor.00g230880.m01
SyntenyCsor.00g230880.m01
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCGACATGTTCTTGAAGCCGAAGTACTACACGAAGTGGTATTGATTCTGATTCTGTTTCTGATTCTGATTCCGATTCCGATTTTTATGGGTTTCTTTTGATTGATTCTTTCTTTTATTGTGGTGTAGCAAATCTTGCTTAAAGCTGTTGAAGTCGCGGCTGGATGCAATTAGGAAGAAGAAGAATACGGTTCTTAAGTATTTGAAGAGCGACATTGTTGAACTTCTCAAGAGTCGTCTCGATTACAATGCTTACAACAGGGTTAGTTCTTCTTCGTGTCTTGGATTAGATTCTTAATCTCTCCGAAACCCATAATTTTAATATCTGTCTTCTGTTGTGATTGTTCTTAAATTCTTTGTGTTTGGATTGACTGTAAGAGAATTTCTGGTTCATCTTCCCTGATTTTGGTCGTGCTTGTTGTGCTAATGGTGAAATAGGCTGAAGGGTTTCTTGTTGAGCGAAATGTGTTGAGATGTTATGAGCTAATTGATGAGTTTTGTGGGACTATCTCCAATCAACTCTCTGTCTTGATCAAACAGAGGTAGTAGATATCATGATTTTTGGAACATGTCTGTGTTCTTAGGCTTCAATTTGTTCAAATCAGTTTAAATGTGGTTTCCATGTTTTTGTTTCTCTTCTAGCGAATGCCCTGATGAATGCAACGAAGCAGTCGCGTCGTTGATCTACGCTGCAGCGAGATTCGCTGACTTGCCTGAATTACGAGAGCTTCGAAACTTCTTTACTGGGAAATATGGATGTTCCTTCGAACCATTTACCAGCATAGAGGTTGTTCTTGGTAGCTTAAGAACTCGGTTGTTTCGTCAGAGGGTCGTCCTAAAACTTGGTTTAATTCTTGTTTTTCCTTTTAGCTTATCGAGAAGTCGAGGGCGACGATTCAATCGAAAGAGATAAAGCTTCAACTACTTCAGGAGATTGCTCAGGAATCCGCCATTGATTGGAACTCCAAAGCTCTTGAACAACAATTGTATATTCCTCCCCCAAATGAAGTGAGTTTGTGTGCAATTCTGTTTTTGTGTGTTGATGAACTCTCTTCATTCTATTGATGGTTTTATGGTCGATCTTGTTGCAGCCTGATGTTGTTCCCGTGCATGAAAGGAAGAAGAGCTCGGTCGGAAAAAGGGACAGCAGCGACGACGAATCCATGTTCGATATTAGAAGTGAAGGCAACACGACCGAGACCTCGACCGTAGATAGTAGTACTGATCAAGATGTCCGAAAAGGAGGTTCCGAGGATGAAGTAGAGGATCAGACACCGTTCTACCTTAGAGTTATCCGACCTCCTTACCTTAAAACAAAACCAACCAACAAGGAAACAATGGAAGAAGCACGAAAAGCTACCGACCAAGTAGAAAACGATGAACGTAATAAGCAGCAATCCGAGCCAACCTCGGAAGAGAAACCGAAACCGAAACCGAAATCAGTCAGGCGAAGAAATGTGAAACTACAGCCTACAGTGGAGATCAAGATCGGCAATGTCGAGAATTCGGGAAACGATATCAGTTCGAGTCGAAACAAAGGCAAAGAAGCCATGAAAGGAGAAGGGGATGATGATGAGGAGGAGGAGAAGATGTTAGATGGGCTGTTGATGCATTACAGTAAGAAAAAACCAAGCCATGAATCAAACGAAGTCAGGGCAGTGTCACTGTCCATAGACTCAACTGAGCCAACTGAGCTAGAGAACAAGCATCATACTAGAACCAATTCGTTTGTTCATCCTAAGCTCCCGGACTACGACAAACTCGCAGCTCGGTTCGCAGCCCTCAAAGAAGATAAATAA

mRNA sequence

ATGTTCGACATGTTCTTGAAGCCGAAGTACTACACGAAGTGCAAATCTTGCTTAAAGCTGTTGAAGTCGCGGCTGGATGCAATTAGGAAGAAGAAGAATACGGTTCTTAAGTATTTGAAGAGCGACATTGTTGAACTTCTCAAGAGTCGTCTCGATTACAATGCTTACAACAGGGCTGAAGGGTTTCTTGTTGAGCGAAATGTGTTGAGATGTTATGAGCTAATTGATGAGTTTTGTGGGACTATCTCCAATCAACTCTCTGTCTTGATCAAACAGAGCGAATGCCCTGATGAATGCAACGAAGCAGTCGCGTCGTTGATCTACGCTGCAGCGAGATTCGCTGACTTGCCTGAATTACGAGAGCTTCGAAACTTCTTTACTGGGAAATATGGATGTTCCTTCGAACCATTTACCAGCATAGAGCTTATCGAGAAGTCGAGGGCGACGATTCAATCGAAAGAGATAAAGCTTCAACTACTTCAGGAGATTGCTCAGGAATCCGCCATTGATTGGAACTCCAAAGCTCTTGAACAACAATTGTATATTCCTCCCCCAAATGAACCTGATGTTGTTCCCGTGCATGAAAGGAAGAAGAGCTCGGTCGGAAAAAGGGACAGCAGCGACGACGAATCCATGTTCGATATTAGAAGTGAAGGCAACACGACCGAGACCTCGACCGTAGATAGTAGTACTGATCAAGATGTCCGAAAAGGAGGTTCCGAGGATGAAGTAGAGGATCAGACACCGTTCTACCTTAGAGTTATCCGACCTCCTTACCTTAAAACAAAACCAACCAACAAGGAAACAATGGAAGAAGCACGAAAAGCTACCGACCAAGTAGAAAACGATGAACGTAATAAGCAGCAATCCGAGCCAACCTCGGAAGAGAAACCGAAACCGAAACCGAAATCAGTCAGGCGAAGAAATGTGAAACTACAGCCTACAGTGGAGATCAAGATCGGCAATGTCGAGAATTCGGGAAACGATATCAGTTCGAGTCGAAACAAAGGCAAAGAAGCCATGAAAGGAGAAGGGGATGATGATGAGGAGGAGGAGAAGATGTTAGATGGGCTGTTGATGCATTACAGTAAGAAAAAACCAAGCCATGAATCAAACGAAGTCAGGGCAGTGTCACTGTCCATAGACTCAACTGAGCCAACTGAGCTAGAGAACAAGCATCATACTAGAACCAATTCGTTTGTTCATCCTAAGCTCCCGGACTACGACAAACTCGCAGCTCGGTTCGCAGCCCTCAAAGAAGATAAATAA

Coding sequence (CDS)

ATGTTCGACATGTTCTTGAAGCCGAAGTACTACACGAAGTGCAAATCTTGCTTAAAGCTGTTGAAGTCGCGGCTGGATGCAATTAGGAAGAAGAAGAATACGGTTCTTAAGTATTTGAAGAGCGACATTGTTGAACTTCTCAAGAGTCGTCTCGATTACAATGCTTACAACAGGGCTGAAGGGTTTCTTGTTGAGCGAAATGTGTTGAGATGTTATGAGCTAATTGATGAGTTTTGTGGGACTATCTCCAATCAACTCTCTGTCTTGATCAAACAGAGCGAATGCCCTGATGAATGCAACGAAGCAGTCGCGTCGTTGATCTACGCTGCAGCGAGATTCGCTGACTTGCCTGAATTACGAGAGCTTCGAAACTTCTTTACTGGGAAATATGGATGTTCCTTCGAACCATTTACCAGCATAGAGCTTATCGAGAAGTCGAGGGCGACGATTCAATCGAAAGAGATAAAGCTTCAACTACTTCAGGAGATTGCTCAGGAATCCGCCATTGATTGGAACTCCAAAGCTCTTGAACAACAATTGTATATTCCTCCCCCAAATGAACCTGATGTTGTTCCCGTGCATGAAAGGAAGAAGAGCTCGGTCGGAAAAAGGGACAGCAGCGACGACGAATCCATGTTCGATATTAGAAGTGAAGGCAACACGACCGAGACCTCGACCGTAGATAGTAGTACTGATCAAGATGTCCGAAAAGGAGGTTCCGAGGATGAAGTAGAGGATCAGACACCGTTCTACCTTAGAGTTATCCGACCTCCTTACCTTAAAACAAAACCAACCAACAAGGAAACAATGGAAGAAGCACGAAAAGCTACCGACCAAGTAGAAAACGATGAACGTAATAAGCAGCAATCCGAGCCAACCTCGGAAGAGAAACCGAAACCGAAACCGAAATCAGTCAGGCGAAGAAATGTGAAACTACAGCCTACAGTGGAGATCAAGATCGGCAATGTCGAGAATTCGGGAAACGATATCAGTTCGAGTCGAAACAAAGGCAAAGAAGCCATGAAAGGAGAAGGGGATGATGATGAGGAGGAGGAGAAGATGTTAGATGGGCTGTTGATGCATTACAGTAAGAAAAAACCAAGCCATGAATCAAACGAAGTCAGGGCAGTGTCACTGTCCATAGACTCAACTGAGCCAACTGAGCTAGAGAACAAGCATCATACTAGAACCAATTCGTTTGTTCATCCTAAGCTCCCGGACTACGACAAACTCGCAGCTCGGTTCGCAGCCCTCAAAGAAGATAAATAA

Protein sequence

MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAEGFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELRELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQLYIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGSEDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKPKPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDDDEEEEKMLDGLLMHYSKKKPSHESNEVRAVSLSIDSTEPTELENKHHTRTNSFVHPKLPDYDKLAARFAALKEDK
Homology
BLAST of Csor.00g230880.m01 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 4.3e-13
Identity = 47/181 (25.97%), Postives = 91/181 (50.28%), Query Frame = 0

Query: 13  KCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAEGFLVERNVLRCY 72
           K K  LKL  SR+  ++ KK  +++  K ++ ELL+ + + +A  R E  + +  ++ C+
Sbjct: 12  KLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRDEYLIECF 71

Query: 73  ELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELRELRNFFTGKYG- 132
           ++I+  C  +  +++++   +E P E  E++ +L+Y++ R   +PEL +++N    KYG 
Sbjct: 72  QIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPELEQIKNQLKAKYGK 131

Query: 133 -------CSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQLYIPP 186
                  C      + +++ K         I  Q L EIA++  +DW         Y PP
Sbjct: 132 GLENEANCHCSTHVNPKIVHKLSYATPDPSIIFQTLSEIAEKFNVDWCGSD-----YPPP 186

BLAST of Csor.00g230880.m01 vs. NCBI nr
Match: KAG6593450.1 (IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 804 bits (2077), Expect = 4.78e-293
Identity = 422/422 (100.00%), Postives = 422/422 (100.00%), Query Frame = 0

Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240
           YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS
Sbjct: 181 YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240

Query: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKP 300
           EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKP
Sbjct: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKP 300

Query: 301 KPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDDDEEEEKMLDGLLM 360
           KPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDDDEEEEKMLDGLLM
Sbjct: 301 KPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDDDEEEEKMLDGLLM 360

Query: 361 HYSKKKPSHESNEVRAVSLSIDSTEPTELENKHHTRTNSFVHPKLPDYDKLAARFAALKE 420
           HYSKKKPSHESNEVRAVSLSIDSTEPTELENKHHTRTNSFVHPKLPDYDKLAARFAALKE
Sbjct: 361 HYSKKKPSHESNEVRAVSLSIDSTEPTELENKHHTRTNSFVHPKLPDYDKLAARFAALKE 420

Query: 421 DK 422
           DK
Sbjct: 421 DK 422

BLAST of Csor.00g230880.m01 vs. NCBI nr
Match: XP_022964044.1 (uncharacterized protein LOC111464187 [Cucurbita moschata] >KAG7025791.1 IST1-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 797 bits (2059), Expect = 2.96e-290
Identity = 421/425 (99.06%), Postives = 422/425 (99.29%), Query Frame = 0

Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240
           YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS
Sbjct: 181 YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240

Query: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKP 300
           EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKP
Sbjct: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKP 300

Query: 301 KPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDDDEEEEKMLDGLLM 360
           KPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDD+EEEEKMLDGLLM
Sbjct: 301 KPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDDEEEEEKMLDGLLM 360

Query: 361 HYSKKKPSHESNEVRAVSLSIDSTEPTEL---ENKHHTRTNSFVHPKLPDYDKLAARFAA 420
           HYSKKKPSHESNEVRAVSLSIDSTEPTEL   ENKHHTRTNSFVHPKLPDYDKLAARFAA
Sbjct: 361 HYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHTRTNSFVHPKLPDYDKLAARFAA 420

Query: 421 LKEDK 422
           LKEDK
Sbjct: 421 LKEDK 425

BLAST of Csor.00g230880.m01 vs. NCBI nr
Match: XP_023514654.1 (uncharacterized protein LOC111778886 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 768 bits (1982), Expect = 1.49e-278
Identity = 409/425 (96.24%), Postives = 413/425 (97.18%), Query Frame = 0

Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKN VLKYLKSDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240
           YIPPPNEPDVVPVH RKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS
Sbjct: 181 YIPPPNEPDVVPVHGRKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240

Query: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKP 300
           EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKAT+QVENDERNKQQ EPT EEKPKP
Sbjct: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATNQVENDERNKQQPEPTLEEKPKP 300

Query: 301 KPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDDDEEEEKMLDGLLM 360
           K  SVRRRNVKLQPT+EIKI NV NSGND+SSSRNKGKEAMKGEGDDDEEEEKMLDGLLM
Sbjct: 301 K--SVRRRNVKLQPTMEIKIDNVGNSGNDMSSSRNKGKEAMKGEGDDDEEEEKMLDGLLM 360

Query: 361 HYSKKKPSHESNEVRAVSLSIDSTEPTEL---ENKHHTRTNSFVHPKLPDYDKLAARFAA 420
           HYSKKKPSHESNEVRAVSLSIDSTEPTEL   ENKHH RTNSFVHPKLPDYD+LAARFAA
Sbjct: 361 HYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHIRTNSFVHPKLPDYDELAARFAA 420

Query: 421 LKEDK 422
           LKEDK
Sbjct: 421 LKEDK 423

BLAST of Csor.00g230880.m01 vs. NCBI nr
Match: XP_023000359.1 (uncharacterized protein LOC111494616 [Cucurbita maxima])

HSP 1 Score: 756 bits (1953), Expect = 3.90e-274
Identity = 401/423 (94.80%), Postives = 409/423 (96.69%), Query Frame = 0

Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF MFLKPKYYTKCKSCLKLLKSRLDAIRKKKN VLKYLKSDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFGMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           GFLVERNVL+CYELIDEFCG ISNQLSVLIKQSECPDE  EAVASLIYAAARFADLPELR
Sbjct: 61  GFLVERNVLKCYELIDEFCGAISNQLSVLIKQSECPDEFKEAVASLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRNFFTGKYGCSFEPFTSIELIEKSRATIQ+KEIKLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQTKEIKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240
           YIPPPNEPDVVP+HERKK SVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS
Sbjct: 181 YIPPPNEPDVVPMHERKKDSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240

Query: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKP 300
           EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDE  KQQ EPTSEEKPKP
Sbjct: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDECKKQQPEPTSEEKPKP 300

Query: 301 KPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDDDEEEE-KMLDGLL 360
           KPKSVRRRNVKLQPT+EIKI +V NSGND+SSSRNKGKEAMKGEGDD+EEEE KMLDGLL
Sbjct: 301 KPKSVRRRNVKLQPTMEIKIDDVGNSGNDMSSSRNKGKEAMKGEGDDEEEEEEKMLDGLL 360

Query: 361 MHYSKKKPSHESNEVRAVSLSIDSTEPTELENKHHTRTNSFVHPKLPDYDKLAARFAALK 420
           MHYSKKKPSHESNEVRAVSLSIDSTEPTELENK HTRTNSFVHP LPDYD+LAARFAALK
Sbjct: 361 MHYSKKKPSHESNEVRAVSLSIDSTEPTELENKRHTRTNSFVHPNLPDYDELAARFAALK 420

Query: 421 EDK 422
           EDK
Sbjct: 421 EDK 423

BLAST of Csor.00g230880.m01 vs. NCBI nr
Match: XP_008462153.1 (PREDICTED: uncharacterized protein LOC103500577 [Cucumis melo])

HSP 1 Score: 503 bits (1295), Expect = 1.55e-173
Identity = 307/466 (65.88%), Postives = 343/466 (73.61%), Query Frame = 0

Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFDMFLKPK+YTKCKSC+K+ K+RLD IRKKKN VLKYLK+DIVELLKSRLDYNA+NRAE
Sbjct: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           GFLVERNVLRCYELIDEFCGTISNQ+ VL K+SECPDEC EAVA+LIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRN FT KYG SF  FT+ E IEKSRAT Q+KE+K+QLLQEIAQE+AIDWNSKALEQQL
Sbjct: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180

Query: 181 YIPPP-NEPD---------------VVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTET 240
           Y PPP NE D                VPV+ERK +S   +++SD+ES+FD RSEGNTTET
Sbjct: 181 YTPPPQNELDGERSGATKRNKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTET 240

Query: 241 STVDSSTDQDVRKGGSEDEVEDQTPFYLRVIRPPYLKTKPTNKETME-EARKATDQVEND 300
           ST DS TDQD+ KG SEDEVEDQ PF  R ++PPYLKTKP   E  E E RK T  +END
Sbjct: 241 STGDS-TDQDIHKGVSEDEVEDQKPFNHRFVQPPYLKTKPIKTEANEKEPRKVT--IEND 300

Query: 301 ERNKQQSEPTSEEKPKPKPKSVRRRNVKLQPTVEIKIGNVENSGND----ISSSRNKGKE 360
           E N  +S PT E   KPKP+SVRRRNVK QP  +I I +V +S  D    ISSSRNKGKE
Sbjct: 301 ESNNPKS-PTEE---KPKPRSVRRRNVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKE 360

Query: 361 AMKGE--GDDDEEEEKMLDGLLMHYSKKKPSHESNE-----------------------V 420
            M GE  G  D+EE ++LDGLLM YSKKK + ES                          
Sbjct: 361 TMIGEEKGARDDEE-RVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTT 420

BLAST of Csor.00g230880.m01 vs. ExPASy TrEMBL
Match: A0A6J1HLZ1 (uncharacterized protein LOC111464187 OS=Cucurbita moschata OX=3662 GN=LOC111464187 PE=3 SV=1)

HSP 1 Score: 797 bits (2059), Expect = 1.44e-290
Identity = 421/425 (99.06%), Postives = 422/425 (99.29%), Query Frame = 0

Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240
           YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS
Sbjct: 181 YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240

Query: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKP 300
           EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKP
Sbjct: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKP 300

Query: 301 KPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDDDEEEEKMLDGLLM 360
           KPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDD+EEEEKMLDGLLM
Sbjct: 301 KPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDDEEEEEKMLDGLLM 360

Query: 361 HYSKKKPSHESNEVRAVSLSIDSTEPTEL---ENKHHTRTNSFVHPKLPDYDKLAARFAA 420
           HYSKKKPSHESNEVRAVSLSIDSTEPTEL   ENKHHTRTNSFVHPKLPDYDKLAARFAA
Sbjct: 361 HYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHTRTNSFVHPKLPDYDKLAARFAA 420

Query: 421 LKEDK 422
           LKEDK
Sbjct: 421 LKEDK 425

BLAST of Csor.00g230880.m01 vs. ExPASy TrEMBL
Match: A0A6J1KJN9 (uncharacterized protein LOC111494616 OS=Cucurbita maxima OX=3661 GN=LOC111494616 PE=3 SV=1)

HSP 1 Score: 756 bits (1953), Expect = 1.89e-274
Identity = 401/423 (94.80%), Postives = 409/423 (96.69%), Query Frame = 0

Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF MFLKPKYYTKCKSCLKLLKSRLDAIRKKKN VLKYLKSDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFGMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           GFLVERNVL+CYELIDEFCG ISNQLSVLIKQSECPDE  EAVASLIYAAARFADLPELR
Sbjct: 61  GFLVERNVLKCYELIDEFCGAISNQLSVLIKQSECPDEFKEAVASLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRNFFTGKYGCSFEPFTSIELIEKSRATIQ+KEIKLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQTKEIKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240
           YIPPPNEPDVVP+HERKK SVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS
Sbjct: 181 YIPPPNEPDVVPMHERKKDSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240

Query: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKP 300
           EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDE  KQQ EPTSEEKPKP
Sbjct: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDECKKQQPEPTSEEKPKP 300

Query: 301 KPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDDDEEEE-KMLDGLL 360
           KPKSVRRRNVKLQPT+EIKI +V NSGND+SSSRNKGKEAMKGEGDD+EEEE KMLDGLL
Sbjct: 301 KPKSVRRRNVKLQPTMEIKIDDVGNSGNDMSSSRNKGKEAMKGEGDDEEEEEEKMLDGLL 360

Query: 361 MHYSKKKPSHESNEVRAVSLSIDSTEPTELENKHHTRTNSFVHPKLPDYDKLAARFAALK 420
           MHYSKKKPSHESNEVRAVSLSIDSTEPTELENK HTRTNSFVHP LPDYD+LAARFAALK
Sbjct: 361 MHYSKKKPSHESNEVRAVSLSIDSTEPTELENKRHTRTNSFVHPNLPDYDELAARFAALK 420

Query: 421 EDK 422
           EDK
Sbjct: 421 EDK 423

BLAST of Csor.00g230880.m01 vs. ExPASy TrEMBL
Match: A0A1S3CGT5 (uncharacterized protein LOC103500577 OS=Cucumis melo OX=3656 GN=LOC103500577 PE=3 SV=1)

HSP 1 Score: 503 bits (1295), Expect = 7.52e-174
Identity = 307/466 (65.88%), Postives = 343/466 (73.61%), Query Frame = 0

Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFDMFLKPK+YTKCKSC+K+ K+RLD IRKKKN VLKYLK+DIVELLKSRLDYNA+NRAE
Sbjct: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           GFLVERNVLRCYELIDEFCGTISNQ+ VL K+SECPDEC EAVA+LIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRN FT KYG SF  FT+ E IEKSRAT Q+KE+K+QLLQEIAQE+AIDWNSKALEQQL
Sbjct: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180

Query: 181 YIPPP-NEPD---------------VVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTET 240
           Y PPP NE D                VPV+ERK +S   +++SD+ES+FD RSEGNTTET
Sbjct: 181 YTPPPQNELDGERSGATKRNKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTET 240

Query: 241 STVDSSTDQDVRKGGSEDEVEDQTPFYLRVIRPPYLKTKPTNKETME-EARKATDQVEND 300
           ST DS TDQD+ KG SEDEVEDQ PF  R ++PPYLKTKP   E  E E RK T  +END
Sbjct: 241 STGDS-TDQDIHKGVSEDEVEDQKPFNHRFVQPPYLKTKPIKTEANEKEPRKVT--IEND 300

Query: 301 ERNKQQSEPTSEEKPKPKPKSVRRRNVKLQPTVEIKIGNVENSGND----ISSSRNKGKE 360
           E N  +S PT E   KPKP+SVRRRNVK QP  +I I +V +S  D    ISSSRNKGKE
Sbjct: 301 ESNNPKS-PTEE---KPKPRSVRRRNVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKE 360

Query: 361 AMKGE--GDDDEEEEKMLDGLLMHYSKKKPSHESNE-----------------------V 420
            M GE  G  D+EE ++LDGLLM YSKKK + ES                          
Sbjct: 361 TMIGEEKGARDDEE-RVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTT 420

BLAST of Csor.00g230880.m01 vs. ExPASy TrEMBL
Match: A0A6J1CWS8 (vacuolar protein sorting-associated protein IST1 OS=Momordica charantia OX=3673 GN=LOC111015509 PE=3 SV=1)

HSP 1 Score: 501 bits (1289), Expect = 4.97e-173
Identity = 295/469 (62.90%), Postives = 329/469 (70.15%), Query Frame = 0

Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFDMF KPK++TKCKSC+KLLK RLD IRKKKN VLK+LK+DI ELL+S LD NAYNRAE
Sbjct: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           G LVERNVL+CY+LIDEF GTI NQLSVL KQSECPDEC EAVASLIYAAARFADLPELR
Sbjct: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELR+ FT KYG SFE FT+ ELIEK  A   +KE+KLQLLQEIAQES I+WNSKALEQQL
Sbjct: 121 ELRSLFTAKYGRSFEQFTNKELIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180

Query: 181 YIPPPNEPDV--------------VPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETST 240
           Y PP N PD               +PV ERK S + K+D+SDD+S+FD RSE N TETST
Sbjct: 181 YTPPQNTPDEEPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTETST 240

Query: 241 VDSSTDQDVRKGGSEDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERN 300
            DSSTDQDV+KG  EDEVED+ PFYLR I PPYLK+KP+ KE   E              
Sbjct: 241 GDSSTDQDVQKGVFEDEVEDRKPFYLRFIPPPYLKSKPSKKEASFE-------------- 300

Query: 301 KQQSEPTSEEKPKPKPKSVRRRNVKLQPTVEIKIGNVENSGND----ISSSRNKGKEAMK 360
               +P  EEKPKP+  SVRRRN KL+PT +I IGNV+NS ND     SSSRNKGKE M 
Sbjct: 301 ----KPAVEEKPKPR--SVRRRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMM 360

Query: 361 GEGDDDEEEEKMLDGLLMHYSKKKPSHESNEV---------------------------- 420
           GE     EEE+MLDGLLMHYSKKK + ESN                              
Sbjct: 361 GEEKGAGEEERMLDGLLMHYSKKKSTQESNRAKGNLKSQKHKEQDEPQRTKNSKAEEDLS 420

BLAST of Csor.00g230880.m01 vs. ExPASy TrEMBL
Match: A0A0A0K926 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G446760 PE=3 SV=1)

HSP 1 Score: 479 bits (1232), Expect = 2.62e-164
Identity = 297/467 (63.60%), Postives = 333/467 (71.31%), Query Frame = 0

Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFDMFLKPK+YTKCKSC+K+ K+RLD  RKKKN VLKYLK+DIVELLKSRLDYNAYNRAE
Sbjct: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTTRKKKNAVLKYLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           GFLVERNVLRCYELIDEFCGTISNQ+ VL K+SECPDEC E+VA+LIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKESVATLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRN FT KYG SF  FT+ E IEKSR T Q+KE+K+QLLQEIAQE+AIDWNSKALEQQL
Sbjct: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRTTTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180

Query: 181 YIPPP-NEPD---------------VVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTET 240
           Y PPP NE D                VPV+E+K +S   +++SD+ES+FD RSEGNTTET
Sbjct: 181 YTPPPENELDGERSGATKRNKTKVVSVPVYEKKANSPRNKNNSDNESIFDSRSEGNTTET 240

Query: 241 STVDSSTDQDVRKGGSEDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEA--RKATDQVEN 300
           ST DS TDQDV KG S DEV DQ PF  R ++PPYLKTKP   E   E   RK T  +EN
Sbjct: 241 STGDS-TDQDVHKGVSGDEV-DQKPFNRRFVQPPYLKTKPIKTEANAEELPRKVT--IEN 300

Query: 301 DERNKQQSEPTSEEKPKPKPKSVRRRNVKLQPTVEIKIGNVENSGND----ISSSRNKGK 360
           DE N     P S  + KPKP+SVRRR VK QP  +I I +V +S  D    ISS RNKGK
Sbjct: 301 DESN-----PKSPTEEKPKPRSVRRRIVKPQPARDINIDDVGSSTVDVTKKISSIRNKGK 360

Query: 361 EAMKGE--GDDDEEEEKMLDGLLMHYSKKKPSHESNE----------------------- 420
           E M GE  G  D+EE ++LDGLLM YSKKK + ES                         
Sbjct: 361 ETMIGEEKGARDDEE-RVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPT 420

BLAST of Csor.00g230880.m01 vs. TAIR 10
Match: AT1G79910.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 230.7 bits (587), Expect = 2.1e-60
Identity = 143/357 (40.06%), Postives = 214/357 (59.94%), Query Frame = 0

Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFD   KPK+YTKCKS +K+ K+R+D +++KKN+V KYLK+DIV+LLK+ LDYNAY RAE
Sbjct: 1   MFDGLFKPKFYTKCKSLVKITKTRVDTVKRKKNSVCKYLKNDIVDLLKNSLDYNAYGRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           G + E+  L CYE +++FC  +++ +S+L K   CPDEC EA++SL+YAAAR +++PELR
Sbjct: 61  GLIEEKRRLACYEFLEQFCNCVASNVSLLQKSIRCPDECREAISSLVYAAARVSEVPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           +LR+ F  +YG + + F + E +E+ +A   SKE+K++LLQEIA+E +I W++K+LEQ+L
Sbjct: 121 DLRSLFAERYGNTLDQFVNPEFVERFKAEPPSKEMKVELLQEIAREYSIKWDAKSLEQRL 180

Query: 181 YIPP--------PNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTT--ETSTVDSS 240
           Y PP          EP     +   + ++ KR S       D   + N +   TS  DS 
Sbjct: 181 YTPPHHHHVHTETEEPKTEKTNSEPEKTMEKRSSLSFHGREDSMDDSNKSIMSTSEEDSM 240

Query: 241 TDQDVRKGGSEDEVEDQTPFYLRVIRP-PYLKTKPTNKETMEEARKATDQVENDERNKQQ 300
           +       G ED+ E   PFY R + P PY K K   +E++ E    +  ++ +      
Sbjct: 241 STSGSCMTGPEDDPE-MKPFYYRFMTPAPYTKPKIEKQESLPEKMTTSHLMDTE------ 300

Query: 301 SEPTSEEKPKPKPKSVRRRNVKLQPTVEIKIGNVE---NSGNDISSSRNKGKEAMKG 344
             PT     KPKP+SVRRR     P  E +   ++    S N + +S + GK + +G
Sbjct: 301 -SPTG---GKPKPRSVRRRFANPPPEAETEGEALKLKVESKNMMRASESMGKASGEG 346

BLAST of Csor.00g230880.m01 vs. TAIR 10
Match: AT1G79910.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 152.5 bits (384), Expect = 7.4e-37
Identity = 105/298 (35.23%), Postives = 164/298 (55.03%), Query Frame = 0

Query: 60  EGFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPEL 119
           EG + E+  L CYE +++FC  +++ +S+L K   CPDEC EA++SL+YAAAR +++PEL
Sbjct: 46  EGLIEEKRRLACYEFLEQFCNCVASNVSLLQKSIRCPDECREAISSLVYAAARVSEVPEL 105

Query: 120 RELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQ 179
           R+LR+ F  +YG + + F + E +E+ +A   SKE+K++LLQEIA+E +I W++K+LEQ+
Sbjct: 106 RDLRSLFAERYGNTLDQFVNPEFVERFKAEPPSKEMKVELLQEIAREYSIKWDAKSLEQR 165

Query: 180 LYIPP--------PNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTT--ETSTVDS 239
           LY PP          EP     +   + ++ KR S       D   + N +   TS  DS
Sbjct: 166 LYTPPHHHHVHTETEEPKTEKTNSEPEKTMEKRSSLSFHGREDSMDDSNKSIMSTSEEDS 225

Query: 240 STDQDVRKGGSEDEVEDQTPFYLRVIRP-PYLKTKPTNKETMEEARKATDQVENDERNKQ 299
            +       G ED+ E   PFY R + P PY K K   +E++ E    +  ++ +     
Sbjct: 226 MSTSGSCMTGPEDDPE-MKPFYYRFMTPAPYTKPKIEKQESLPEKMTTSHLMDTE----- 285

Query: 300 QSEPTSEEKPKPKPKSVRRRNVKLQPTVEIKIGNVE---NSGNDISSSRNKGKEAMKG 344
              PT     KPKP+SVRRR     P  E +   ++    S N + +S + GK + +G
Sbjct: 286 --SPTG---GKPKPRSVRRRFANPPPEAETEGEALKLKVESKNMMRASESMGKASGEG 332

BLAST of Csor.00g230880.m01 vs. TAIR 10
Match: AT4G32350.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 138.7 bits (348), Expect = 1.1e-32
Identity = 82/223 (36.77%), Postives = 130/223 (58.30%), Query Frame = 0

Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFD FL   +  K K  +KL K+R+D +R+K+N  +K+LK D+ +L+ +  DYNA++RA 
Sbjct: 1   MFDGFLGRGFAPKGKPLIKLTKNRIDVLRRKRNATIKFLKRDLADLIINGHDYNAFSRAG 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           G L E   L   + +++ C  +  QLS + K  ECP++C EA++SL++AA+ F++LPELR
Sbjct: 61  GLLDELRYLWSLDFVEQTCDFVYKQLSTMQKTPECPEDCREAISSLMFAASGFSELPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELR  F  KY  S   F + EL+E   +   S E K++L++++A E +I W+SK  E+++
Sbjct: 121 ELRQMFHEKYTDSLALFVNQELVENMSSKPFSMEKKVKLMEDVALEFSIRWDSKDFEKRI 180

Query: 181 YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTE 224
                   + + V E  KS+  K    D       R E   +E
Sbjct: 181 V-----RQNSISVMETPKSTNDKYKPVDRNMALPKREEFEGSE 218

BLAST of Csor.00g230880.m01 vs. TAIR 10
Match: AT1G52315.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 136.7 bits (343), Expect = 4.2e-32
Identity = 97/304 (31.91%), Postives = 164/304 (53.95%), Query Frame = 0

Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF    KPK+Y K KS    +K R+D +R+K+  +++  K+DIV  LK+  D  AY RAE
Sbjct: 1   MFLRCFKPKFYKKSKSTTSYMKIRIDIVRRKRIAMVRNYKTDIVNFLKNGQDSEAYRRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
             L E  ++ CY+LI+ FC  IS  LS+++K+ ECP+EC EAV+SLIYA A   D+PEL+
Sbjct: 61  LLLEELRIISCYDLIERFCDCISENLSLMLKKRECPEECREAVSSLIYATAWVPDVPELK 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSR-ATIQSKEIKLQLLQEIAQESAIDWNSKALEQQ 180
           +LR  FT ++G       + EL+EK+      S+E+K+Q ++++A E +I+W+   L+  
Sbjct: 121 DLRAVFTKRFGNFIASSVNHELVEKTELLRPPSRELKIQTVKDVANEFSINWDPTPLKLM 180

Query: 181 LYIPPPNEPDVVPVHERKKSSVG----------KRDSSDDESM------FDIRSEGNTTE 240
           L      E   + + ++ ++               + SDDES+       D  S+G+ + 
Sbjct: 181 LL----RETSALQIKDKVETRADYLNKDTEKSFMDEHSDDESVLSESWRIDSLSKGSLSS 240

Query: 241 TSTVDSSTDQDVRKGGSEDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVEND 288
           +S+  SS+ + V    S  + + +      +I  P  K    N E  +E ++   ++ + 
Sbjct: 241 SSSSSSSSLRRV----SVRKKKGKKILPYGIISSPDTKLGAGNDEKAQEEKEEKRKIADQ 296

BLAST of Csor.00g230880.m01 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 111.3 bits (277), Expect = 1.9e-24
Identity = 116/440 (26.36%), Postives = 208/440 (47.27%), Query Frame = 0

Query: 12  TKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAEGFLVERNVLRC 71
           +KCK+  K+  +R+  IR K+  V+K ++ DI  LL+S  D  A  R E  + E+N+   
Sbjct: 29  SKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHVIREQNIQAA 88

Query: 72  YELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELRELRNFFTGKYG 131
            E+I+ FC  I ++L+++ KQ +CP +  E +ASLI+AA R +++PEL +LR+ F  KYG
Sbjct: 89  NEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDLRDIFAKKYG 148

Query: 132 CSF-------EPFTSIE--LIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQLYI 191
             F        P   +   LI+K        E KL++++EIA+E  +DW++   EQ+L +
Sbjct: 149 KDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAKEFQVDWDTTETEQEL-L 208

Query: 192 PPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGSED 251
            P  E    P      SS+    ++ +E +   ++   +T + ++++          +  
Sbjct: 209 KPQEESIDGPRKFVSASSLPVNRAAINEPIDPTKAVPRSTSSMSINTHYHDTESAAEAAT 268

Query: 252 EVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVEN-----------DERNKQQSE 311
           E+  Q     +V      +   +NKE    +  +T Q ++             R  + SE
Sbjct: 269 ELAKQAVAAAQVASLLATRRDSSNKEFSVSSDHSTHQKDSQYMDHHHHHPGSRRQSRDSE 328

Query: 312 PTS-EEKPKPKPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDDDEE 371
            +S   KP  + + + RR+    P +     N  +   + +++  + KE M+     +  
Sbjct: 329 TSSYYAKPGAENRGMGRRHSYNNPGI-----NESDYEEEYTNTEAEAKETMRRRHSYNPR 388

Query: 372 E------EKMLDGLLMHYSKKKPSHESNEVRAVSLSIDSTEPTELENKHHTRTNS--FVH 423
                   ++      +Y ++    E ++ R  SL  +   P   ++    + +S   VH
Sbjct: 389 SVPPPATSEIKFDESDYYEEETEPDEPSQGRVSSLPPNRAPPQAPQSGESRQDSSGHQVH 448

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q54I394.3e-1325.97IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KAG6593450.14.78e-293100.00IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022964044.12.96e-29099.06uncharacterized protein LOC111464187 [Cucurbita moschata] >KAG7025791.1 IST1-lik... [more]
XP_023514654.11.49e-27896.24uncharacterized protein LOC111778886 [Cucurbita pepo subsp. pepo][more]
XP_023000359.13.90e-27494.80uncharacterized protein LOC111494616 [Cucurbita maxima][more]
XP_008462153.11.55e-17365.88PREDICTED: uncharacterized protein LOC103500577 [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A6J1HLZ11.44e-29099.06uncharacterized protein LOC111464187 OS=Cucurbita moschata OX=3662 GN=LOC1114641... [more]
A0A6J1KJN91.89e-27494.80uncharacterized protein LOC111494616 OS=Cucurbita maxima OX=3661 GN=LOC111494616... [more]
A0A1S3CGT57.52e-17465.88uncharacterized protein LOC103500577 OS=Cucumis melo OX=3656 GN=LOC103500577 PE=... [more]
A0A6J1CWS84.97e-17362.90vacuolar protein sorting-associated protein IST1 OS=Momordica charantia OX=3673 ... [more]
A0A0A0K9262.62e-16463.60Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G446760 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G79910.12.1e-6040.06Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G79910.27.4e-3735.23Regulator of Vps4 activity in the MVB pathway protein [more]
AT4G32350.11.1e-3236.77Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G52315.14.2e-3231.91Regulator of Vps4 activity in the MVB pathway protein [more]
AT4G35730.11.9e-2426.36Regulator of Vps4 activity in the MVB pathway protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 266..286
NoneNo IPR availableCOILSCoilCoilcoord: 143..163
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 319..334
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 190..216
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 217..233
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 381..403
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 265..302
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 234..249
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 182..352
NoneNo IPR availablePANTHERPTHR12161:SF60REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEINcoord: 9..420
IPR005061Vacuolar protein sorting-associated protein Ist1PFAMPF03398Ist1coord: 15..169
e-value: 1.6E-39
score: 135.5
IPR005061Vacuolar protein sorting-associated protein Ist1PANTHERPTHR12161IST1 FAMILY MEMBERcoord: 9..420
IPR042277Vacuolar protein sorting-associated protein IST1-likeGENE3D1.20.1260.60coord: 6..177
e-value: 1.6E-45
score: 156.9

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Csor.00g230880Csor.00g230880gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g230880.m01.CDS01Csor.00g230880.m01.CDS01CDS
Csor.00g230880.m01.CDS02Csor.00g230880.m01.CDS02CDS
Csor.00g230880.m01.CDS03Csor.00g230880.m01.CDS03CDS
Csor.00g230880.m01.CDS04Csor.00g230880.m01.CDS04CDS
Csor.00g230880.m01.CDS05Csor.00g230880.m01.CDS05CDS
Csor.00g230880.m01.CDS06Csor.00g230880.m01.CDS06CDS


The following stop_codon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g230880.m01.stop_codon01Csor.00g230880.m01.stop_codon01stop_codon


The following terminal feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g230880.m01.terminal01Csor.00g230880.m01.terminal01terminal


The following intron feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g230880.m01.intron01Csor.00g230880.m01.intron01intron
Csor.00g230880.m01.intron02Csor.00g230880.m01.intron02intron
Csor.00g230880.m01.intron03Csor.00g230880.m01.intron03intron
Csor.00g230880.m01.intron04Csor.00g230880.m01.intron04intron
Csor.00g230880.m01.intron05Csor.00g230880.m01.intron05intron


The following internal feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g230880.m01.internal01Csor.00g230880.m01.internal01internal
Csor.00g230880.m01.internal02Csor.00g230880.m01.internal02internal
Csor.00g230880.m01.internal03Csor.00g230880.m01.internal03internal
Csor.00g230880.m01.internal04Csor.00g230880.m01.internal04internal


The following initial feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g230880.m01.initial01Csor.00g230880.m01.initial01initial


The following start_codon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g230880.m01.start_codon01Csor.00g230880.m01.start_codon01start_codon


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Csor.00g230880.m01Csor.00g230880.m01-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015031 protein transport