Csor.00g106120.m01 (mRNA) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g106120.m01
TypemRNA
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionnifU-like protein 1, chloroplastic
LocationCsor_Chr17: 8621505 .. 8623464 (-)
Sequence length663
RNA-Seq ExpressionCsor.00g106120.m01
SyntenyCsor.00g106120.m01
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTCTTTAACTATCTCCGGCCTCCTCAAAACCCCTCTTTATTATTCCCAAGCTCTTGCAAATCACCGCCAGTACCCACCTTCTGCTTTTGGGCAACATCAAATACCCGTTTCCCTGAATAGGGTGTTGCCCAGACAAGCCATTAGAGCTTCCGCTTCGAGTTCTGGTCCATCCACGAGCTCCTCGCCAGGGCTGTACTCCGCCCAGAAATTCGAACTGACCACTGCAAACGTAGACTTGGTTCTTGAGGACGTTAGGCCCTATCTAATCGCAGATGGAGGAAATGTCGATGTCGTGTCCGTTGAAGATGGAATCGTTTCTCTCAAACTCGTTGGTGGGTGAATTCCTGATCTCATTTCATTGAATGGGTTTGTTTTAATCTTGTTTGTTAATTTAGCTTTGTGTATTGAAATTTTTGTAATTGAATGATTGGTTGCTACTGCTTATGTGCGCGAAATGTTGATGAACAATCGAATAGGTTAGGGAGTTTATGCCTCAGAAAATTCTAAATTCTTCATTTGGGCGTTTCTTAGACCAAGAGTTATAGGATCCCTAGAAGTTGAAGACATCAAAGAACTCTTAGTTCCATGGCCTTGGTACTTAGAAGCTCAAACACCACTTCATGAAGATACTGTATCCTTTTACCATCCAGCTTCTCAATTCTTATATGTTTAGGTTCTGATCATATCTTTTGCTCTCTGCCCCATTGCCTAGAGTTTTCAGGAGATTTTTTTTGGGAGATCATATTTGATCATATTCCATAGTCCCACCATCTCCCCAGTACAGTTTGGCTCAGCTCATAACATCAAAACCCACCGCTAACAGATATTGTCCACTTTGGTCCGTTATGTATCGCTGTCAGCCTCATGGGGTTAAAACGCGTCTATTAGGGAGAGGTTTCCACACCCTTATAAAGAGTGCTTTATTCCCTCTCCAACCGATGTGGGATCTCACAATCCACCCCCCTTGGGGGCCTACGTCTTCGCTAGTACAGTACTCGGTGTTTGGCTCTAATACCATTTGTAACAGTCCAAGCCCACCGCTAACAAATATTGTCTACTTTAGCTTGTTACGGACCGTTGTCAGTCTCAAAGTTTTAAAACGTGTCTGCTAGGGAGAGGTTTTCACGTTCTTATGAAAAATGTTTCGTTCCTCTCTCCAACCGATGTGAGATCTCACGCAGCTAGTTTAAAACTTTTCCTTATTGACTAGTTGAAGAGCATTTATTTGTTATCTCGGTCCAGACTGGGTTTGGACCTCTAGTACCTGCCTCGACACGTGCCTGGTGAGTTTATCTGTTTCATCTGCTGTAGGGGAAAACGACTCCTTGAAGTAGGTGGGCAAAAGGTAGTGAGCATGTAATCTTCACTATCTTGTTAGACATCTTGCAGCAGATACCTGAGTTTATCTTTGAGTTTTGAGCTTAGATTCCATTTAGTCCATAAGCTTCAAGATTTTACACTTTTTTTTTTAACTAAAGACTTAGCAAGAATCAAATCAATCCTCAGGAGCATGCGGTAGCTGCCCGAGCTCAACAACCACCATGAAGATGGGAATCGAACGAGTACTGAAGGAAAAGTTTGGAGATTCAGTGAAGGAAATATGCCAAGTATATGATGAAGAGCCAAAGGAAATAACAACCGAGGTGAGTTTTCTCTGGACGAACGAACGAATACAAAAGAACTCGAACATATTTCGATGTTATGTTAAATTCAAACAAAAACAGAGAGATGAATTAGTATTAACGTTGGATGTTGCATCAATGCAGGCAGTAAATAGTCATCTTGACATATTGAGGCCAGCCATAAGGAACTATGGCGGGAGCGTGGAAGTAATATCGATCGATGGTGGCAATTGCGTCGTTAAGTATGAGGGTCCTGAATCTATTGGTACAGGAATTAAAGCAGCCATTAAGGAGAAGTTCCCAGATATTACAAACGTTGTGTTTTCAAGCTAG

mRNA sequence

ATGGCGTCTTTAACTATCTCCGGCCTCCTCAAAACCCCTCTTTATTATTCCCAAGCTCTTGCAAATCACCGCCAGTACCCACCTTCTGCTTTTGGGCAACATCAAATACCCGTTTCCCTGAATAGGGTGTTGCCCAGACAAGCCATTAGAGCTTCCGCTTCGAGTTCTGGTCCATCCACGAGCTCCTCGCCAGGGCTGTACTCCGCCCAGAAATTCGAACTGACCACTGCAAACGTAGACTTGGTTCTTGAGGACGTTAGGCCCTATCTAATCGCAGATGGAGGAAATGTCGATGTCGTGTCCGTTGAAGATGGAATCGTTTCTCTCAAACTCGTTGGAGCATGCGGTAGCTGCCCGAGCTCAACAACCACCATGAAGATGGGAATCGAACGAGTACTGAAGGAAAAGTTTGGAGATTCAGTGAAGGAAATATGCCAAGTATATGATGAAGAGCCAAAGGAAATAACAACCGAGGCAGTAAATAGTCATCTTGACATATTGAGGCCAGCCATAAGGAACTATGGCGGGAGCGTGGAAGTAATATCGATCGATGGTGGCAATTGCGTCGTTAAGTATGAGGGTCCTGAATCTATTGGTACAGGAATTAAAGCAGCCATTAAGGAGAAGTTCCCAGATATTACAAACGTTGTGTTTTCAAGCTAG

Coding sequence (CDS)

ATGGCGTCTTTAACTATCTCCGGCCTCCTCAAAACCCCTCTTTATTATTCCCAAGCTCTTGCAAATCACCGCCAGTACCCACCTTCTGCTTTTGGGCAACATCAAATACCCGTTTCCCTGAATAGGGTGTTGCCCAGACAAGCCATTAGAGCTTCCGCTTCGAGTTCTGGTCCATCCACGAGCTCCTCGCCAGGGCTGTACTCCGCCCAGAAATTCGAACTGACCACTGCAAACGTAGACTTGGTTCTTGAGGACGTTAGGCCCTATCTAATCGCAGATGGAGGAAATGTCGATGTCGTGTCCGTTGAAGATGGAATCGTTTCTCTCAAACTCGTTGGAGCATGCGGTAGCTGCCCGAGCTCAACAACCACCATGAAGATGGGAATCGAACGAGTACTGAAGGAAAAGTTTGGAGATTCAGTGAAGGAAATATGCCAAGTATATGATGAAGAGCCAAAGGAAATAACAACCGAGGCAGTAAATAGTCATCTTGACATATTGAGGCCAGCCATAAGGAACTATGGCGGGAGCGTGGAAGTAATATCGATCGATGGTGGCAATTGCGTCGTTAAGTATGAGGGTCCTGAATCTATTGGTACAGGAATTAAAGCAGCCATTAAGGAGAAGTTCCCAGATATTACAAACGTTGTGTTTTCAAGCTAG

Protein sequence

MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPSTSSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEVISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS
Homology
BLAST of Csor.00g106120.m01 vs. ExPASy Swiss-Prot
Match: Q93W77 (NifU-like protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU1 PE=1 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 6.6e-66
Identity = 134/195 (68.72%), Postives = 156/195 (80.00%), Query Frame = 0

Query: 31  FGQHQIPVSLNRVLPRQAIRASASSSGPSTSS-----SPGLYSAQKFELTTANVDLVLED 90
           F   +  +S   +  R AI  S SS G   S      S GLYSAQ F+LT  NVDLVLED
Sbjct: 39  FANKRRHISRTAIFHRSAI--SGSSQGEKISPLASGVSSGLYSAQTFDLTPQNVDLVLED 98

Query: 91  VRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKMGIERVLKEKFGDSVKEIC 150
           VRP+LI+DGGNVDVVSVEDG+VSLKL GAC SCPSS+TTM MGIERVLKEKFGD++K+I 
Sbjct: 99  VRPFLISDGGNVDVVSVEDGVVSLKLQGACTSCPSSSTTMTMGIERVLKEKFGDALKDIR 158

Query: 151 QVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEVISIDGGNCVVKYEGPESIGTGIKAA 210
           QV+DEE K+IT EAVN+HLDILRPAI+NYGGSVEV+S++G +CVVKY GPESIG GI+AA
Sbjct: 159 QVFDEEVKQITVEAVNAHLDILRPAIKNYGGSVEVLSVEGEDCVVKYVGPESIGMGIQAA 218

Query: 211 IKEKFPDITNVVFSS 221
           IKEKF DI+NV F+S
Sbjct: 219 IKEKFKDISNVTFTS 231

BLAST of Csor.00g106120.m01 vs. ExPASy Swiss-Prot
Match: Q93W20 (NifU-like protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU2 PE=1 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 8.5e-21
Identity = 58/148 (39.19%), Postives = 86/148 (58.11%), Query Frame = 0

Query: 74  LTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKMGIERVL 133
           LT  NV+ VL+++RPYL++DGGNV +  ++  IV +KL GACGSCPSST TMKMGIER L
Sbjct: 85  LTEENVESVLDEIRPYLMSDGGNVALHEIDGNIVRVKLQGACGSCPSSTMTMKMGIERRL 144

Query: 134 KEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRP-AIRNYGGSVEVISIDGGNCVVKY 193
            EK  + V       +E   E+  E +   L+ +RP  I    GS++++ I+     ++ 
Sbjct: 145 MEKIPEIVAVEALPDEETGLELNEENIEKVLEEIRPYLIGTADGSLDLVEIEDPIVKIRI 204

Query: 194 EGPE----SIGTGIKAAIKEKFPDITNV 217
            GP     ++   +   ++EK P I  V
Sbjct: 205 TGPAAGVMTVRVAVTQKLREKIPSIAAV 232

BLAST of Csor.00g106120.m01 vs. ExPASy Swiss-Prot
Match: Q84LK7 (NifU-like protein 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NIFU1 PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 2.7e-19
Identity = 62/152 (40.79%), Postives = 87/152 (57.24%), Query Frame = 0

Query: 68  SAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKM 127
           +A +  LT  NV+ VL+ VRPYL ADGG+V +  +   +V LKL GACGSCPSS  T+K 
Sbjct: 69  TAVQLPLTAGNVESVLDQVRPYLTADGGDVALHEIAGNVVRLKLQGACGSCPSSLITIKR 128

Query: 128 GIERVLKEKFGDSVKEICQVYDEEP-KEITTEAVNSHLDILRPAIRNY-GGSVEVISIDG 187
           GIER L EK  D V  +  V D+E   E+  E V   L+ +RP +    GG ++ + I G
Sbjct: 129 GIERRLMEKIPD-VAAVEPVTDKETGLELNEENVEKVLNEIRPYLAGTGGGGLQFLMIKG 188

Query: 188 GNCVVKYEGPESIGTGIKAAI----KEKFPDI 214
               V+  GP ++   ++ A+    +EK P I
Sbjct: 189 PIVKVRLTGPAAVVRTVRIAVSKKLREKIPSI 219

BLAST of Csor.00g106120.m01 vs. ExPASy Swiss-Prot
Match: Q84RQ7 (NifU-like protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU3 PE=2 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 2.3e-18
Identity = 56/139 (40.29%), Postives = 84/139 (60.43%), Query Frame = 0

Query: 74  LTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKMGIERVL 133
           LT  NV+ VL++VRP L+ADGGNV +  ++  +V LKL GACGSCPSS+ T+KMGIE  L
Sbjct: 84  LTEENVERVLDEVRPSLMADGGNVALHEIDGLVVVLKLQGACGSCPSSSMTLKMGIESRL 143

Query: 134 KEKFGDSVKEICQVYDEEP--KEITTEAVNSHLDILRPAIRNY-GGSVEVISIDGGNCVV 193
           ++K  + +  + Q  + E    E+  E +   L  LRP +    GG +E++ IDG    V
Sbjct: 144 RDKIPE-IMSVEQFLESETGGLELNDENIEKVLSELRPYLSGTGGGGLELVEIDGYVVKV 203

Query: 194 KYEGPESIGTGIKAAIKEK 210
           +  GP +    ++ A+ +K
Sbjct: 204 RLTGPAAGVMTVRVALTQK 221

BLAST of Csor.00g106120.m01 vs. ExPASy Swiss-Prot
Match: O32119 (Putative nitrogen fixation protein YutI OS=Bacillus subtilis (strain 168) OX=224308 GN=yutI PE=3 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 5.0e-13
Identity = 38/70 (54.29%), Postives = 53/70 (75.71%), Query Frame = 0

Query: 79  VDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKMGIERVLKEKFG 138
           V  VL+ +RP+L+ DGG+ ++V V++GIV L+L+GACGSCPSST T+K GIER L E+  
Sbjct: 43  VQEVLDKLRPFLLRDGGDCELVDVDEGIVKLRLLGACGSCPSSTITLKAGIERALLEEV- 102

Query: 139 DSVKEICQVY 149
             V E+ QV+
Sbjct: 103 PGVVEVEQVF 111

BLAST of Csor.00g106120.m01 vs. NCBI nr
Match: XP_022953177.1 (nifU-like protein 1, chloroplastic [Cucurbita moschata] >KAG6575747.1 NifU-like protein 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014298.1 NifU-like protein 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 427 bits (1099), Expect = 8.95e-151
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 0

Query: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPST 60
           MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPST
Sbjct: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPST 60

Query: 61  SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120
           SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180

Query: 181 ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS 220
           ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS
Sbjct: 181 ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS 220

BLAST of Csor.00g106120.m01 vs. NCBI nr
Match: XP_022991837.1 (nifU-like protein 1, chloroplastic [Cucurbita maxima])

HSP 1 Score: 419 bits (1077), Expect = 2.02e-147
Identity = 215/220 (97.73%), Postives = 217/220 (98.64%), Query Frame = 0

Query: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPST 60
           MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNR LPRQ+IRASAS+SGPST
Sbjct: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRALPRQSIRASASNSGPST 60

Query: 61  SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120
           SSSPGLYSAQKFELT ANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180

Query: 181 ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS 220
           ISIDGGNCVV YEGPESIGTGIKAAIKEKFPDITNVVFSS
Sbjct: 181 ISIDGGNCVVMYEGPESIGTGIKAAIKEKFPDITNVVFSS 220

BLAST of Csor.00g106120.m01 vs. NCBI nr
Match: XP_023547622.1 (nifU-like protein 1, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 418 bits (1075), Expect = 4.08e-147
Identity = 215/220 (97.73%), Postives = 216/220 (98.18%), Query Frame = 0

Query: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPST 60
           MASLTISGLLKTPLYYSQALANHRQYPP +FGQHQIPV LNR LP QAIRASASSSGPST
Sbjct: 1   MASLTISGLLKTPLYYSQALANHRQYPPFSFGQHQIPVCLNRALPSQAIRASASSSGPST 60

Query: 61  SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120
           SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180

Query: 181 ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS 220
           ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS
Sbjct: 181 ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS 220

BLAST of Csor.00g106120.m01 vs. NCBI nr
Match: XP_022144330.1 (nifU-like protein 1, chloroplastic [Momordica charantia])

HSP 1 Score: 379 bits (972), Expect = 2.19e-131
Identity = 195/219 (89.04%), Postives = 204/219 (93.15%), Query Frame = 0

Query: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPST 60
           MASLT SGLLKTP  YSQ  AN+RQYPPSA  QHQ P SL RVLPR+ I+ASAS+SGPST
Sbjct: 1   MASLTTSGLLKTPPCYSQIPANYRQYPPSAHRQHQGPNSLKRVLPRRGIKASASNSGPST 60

Query: 61  SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120
           SSSPGLYSAQKFELT  NVDLVLEDVRPYLIADGGNVDVVSVEDG+VSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKE T EAVNSHLD+LRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDVLRPAIRNYGGSVEV 180

Query: 181 ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFS 219
           ISIDGG+C+VKYEGPESIGTGIKAAIKE+FPDITNVVFS
Sbjct: 181 ISIDGGDCLVKYEGPESIGTGIKAAIKERFPDITNVVFS 219

BLAST of Csor.00g106120.m01 vs. NCBI nr
Match: XP_008451442.1 (PREDICTED: nifU-like protein 1, chloroplastic [Cucumis melo])

HSP 1 Score: 378 bits (970), Expect = 4.12e-131
Identity = 192/220 (87.27%), Postives = 205/220 (93.18%), Query Frame = 0

Query: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPST 60
           MASLT SGLLKTPL  SQ+ AN+ QYPP A+GQHQ+PVSL R  P +A+RAS S+SGPST
Sbjct: 1   MASLTTSGLLKTPLKNSQSPANYFQYPPFAYGQHQLPVSLKRAFPGRAVRASPSNSGPST 60

Query: 61  SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120
           SSSPGLYSAQKFELT  NVDLVLEDVRPYLIADGGNVDVVSVEDG+VSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKE T EAVN+HLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNNHLDILRPAIRNYGGSVEV 180

Query: 181 ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS 220
           ISI+GGNC+VKYEGPESIGTG+KAAIKE+FPDITNVVFSS
Sbjct: 181 ISINGGNCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 220

BLAST of Csor.00g106120.m01 vs. ExPASy TrEMBL
Match: A0A6J1GMI1 (nifU-like protein 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111455795 PE=3 SV=1)

HSP 1 Score: 427 bits (1099), Expect = 4.33e-151
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 0

Query: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPST 60
           MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPST
Sbjct: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPST 60

Query: 61  SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120
           SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180

Query: 181 ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS 220
           ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS
Sbjct: 181 ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS 220

BLAST of Csor.00g106120.m01 vs. ExPASy TrEMBL
Match: A0A6J1JN01 (nifU-like protein 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111488364 PE=3 SV=1)

HSP 1 Score: 419 bits (1077), Expect = 9.79e-148
Identity = 215/220 (97.73%), Postives = 217/220 (98.64%), Query Frame = 0

Query: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPST 60
           MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNR LPRQ+IRASAS+SGPST
Sbjct: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRALPRQSIRASASNSGPST 60

Query: 61  SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120
           SSSPGLYSAQKFELT ANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180

Query: 181 ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS 220
           ISIDGGNCVV YEGPESIGTGIKAAIKEKFPDITNVVFSS
Sbjct: 181 ISIDGGNCVVMYEGPESIGTGIKAAIKEKFPDITNVVFSS 220

BLAST of Csor.00g106120.m01 vs. ExPASy TrEMBL
Match: A0A6J1CRB9 (nifU-like protein 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111014036 PE=3 SV=1)

HSP 1 Score: 379 bits (972), Expect = 1.06e-131
Identity = 195/219 (89.04%), Postives = 204/219 (93.15%), Query Frame = 0

Query: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPST 60
           MASLT SGLLKTP  YSQ  AN+RQYPPSA  QHQ P SL RVLPR+ I+ASAS+SGPST
Sbjct: 1   MASLTTSGLLKTPPCYSQIPANYRQYPPSAHRQHQGPNSLKRVLPRRGIKASASNSGPST 60

Query: 61  SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120
           SSSPGLYSAQKFELT  NVDLVLEDVRPYLIADGGNVDVVSVEDG+VSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKE T EAVNSHLD+LRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDVLRPAIRNYGGSVEV 180

Query: 181 ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFS 219
           ISIDGG+C+VKYEGPESIGTGIKAAIKE+FPDITNVVFS
Sbjct: 181 ISIDGGDCLVKYEGPESIGTGIKAAIKERFPDITNVVFS 219

BLAST of Csor.00g106120.m01 vs. ExPASy TrEMBL
Match: A0A1S3BQX1 (nifU-like protein 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492737 PE=3 SV=1)

HSP 1 Score: 378 bits (970), Expect = 1.99e-131
Identity = 192/220 (87.27%), Postives = 205/220 (93.18%), Query Frame = 0

Query: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPST 60
           MASLT SGLLKTPL  SQ+ AN+ QYPP A+GQHQ+PVSL R  P +A+RAS S+SGPST
Sbjct: 1   MASLTTSGLLKTPLKNSQSPANYFQYPPFAYGQHQLPVSLKRAFPGRAVRASPSNSGPST 60

Query: 61  SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120
           SSSPGLYSAQKFELT  NVDLVLEDVRPYLIADGGNVDVVSVEDG+VSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKE T EAVN+HLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNNHLDILRPAIRNYGGSVEV 180

Query: 181 ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS 220
           ISI+GGNC+VKYEGPESIGTG+KAAIKE+FPDITNVVFSS
Sbjct: 181 ISINGGNCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 220

BLAST of Csor.00g106120.m01 vs. ExPASy TrEMBL
Match: A0A5A7TT28 (NifU-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001900 PE=3 SV=1)

HSP 1 Score: 376 bits (965), Expect = 1.15e-130
Identity = 191/220 (86.82%), Postives = 205/220 (93.18%), Query Frame = 0

Query: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPST 60
           MASLT SGLLKTPL  SQ+ AN+ QYPP A+GQHQ+PVSL R  P +A+RAS S+SGPST
Sbjct: 1   MASLTTSGLLKTPLKNSQSPANYFQYPPFAYGQHQLPVSLKRAFPGRAVRASPSNSGPST 60

Query: 61  SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120
           SSSPGLYSAQKFELT  NVDLVLEDVRPYLIADGGNVDVVSVEDG+VSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKE T EAVN+HLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNNHLDILRPAIRNYGGSVEV 180

Query: 181 ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS 220
           ISI+GG+C+VKYEGPESIGTG+KAAIKE+FPDITNVVFSS
Sbjct: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 220

BLAST of Csor.00g106120.m01 vs. TAIR 10
Match: AT4G01940.1 (NFU domain protein 1 )

HSP 1 Score: 251.9 bits (642), Expect = 4.7e-67
Identity = 134/195 (68.72%), Postives = 156/195 (80.00%), Query Frame = 0

Query: 31  FGQHQIPVSLNRVLPRQAIRASASSSGPSTSS-----SPGLYSAQKFELTTANVDLVLED 90
           F   +  +S   +  R AI  S SS G   S      S GLYSAQ F+LT  NVDLVLED
Sbjct: 39  FANKRRHISRTAIFHRSAI--SGSSQGEKISPLASGVSSGLYSAQTFDLTPQNVDLVLED 98

Query: 91  VRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKMGIERVLKEKFGDSVKEIC 150
           VRP+LI+DGGNVDVVSVEDG+VSLKL GAC SCPSS+TTM MGIERVLKEKFGD++K+I 
Sbjct: 99  VRPFLISDGGNVDVVSVEDGVVSLKLQGACTSCPSSSTTMTMGIERVLKEKFGDALKDIR 158

Query: 151 QVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEVISIDGGNCVVKYEGPESIGTGIKAA 210
           QV+DEE K+IT EAVN+HLDILRPAI+NYGGSVEV+S++G +CVVKY GPESIG GI+AA
Sbjct: 159 QVFDEEVKQITVEAVNAHLDILRPAIKNYGGSVEVLSVEGEDCVVKYVGPESIGMGIQAA 218

Query: 211 IKEKFPDITNVVFSS 221
           IKEKF DI+NV F+S
Sbjct: 219 IKEKFKDISNVTFTS 231

BLAST of Csor.00g106120.m01 vs. TAIR 10
Match: AT5G49940.1 (NIFU-like protein 2 )

HSP 1 Score: 102.1 bits (253), Expect = 6.0e-22
Identity = 58/148 (39.19%), Postives = 86/148 (58.11%), Query Frame = 0

Query: 74  LTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKMGIERVL 133
           LT  NV+ VL+++RPYL++DGGNV +  ++  IV +KL GACGSCPSST TMKMGIER L
Sbjct: 85  LTEENVESVLDEIRPYLMSDGGNVALHEIDGNIVRVKLQGACGSCPSSTMTMKMGIERRL 144

Query: 134 KEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRP-AIRNYGGSVEVISIDGGNCVVKY 193
            EK  + V       +E   E+  E +   L+ +RP  I    GS++++ I+     ++ 
Sbjct: 145 MEKIPEIVAVEALPDEETGLELNEENIEKVLEEIRPYLIGTADGSLDLVEIEDPIVKIRI 204

Query: 194 EGPE----SIGTGIKAAIKEKFPDITNV 217
            GP     ++   +   ++EK P I  V
Sbjct: 205 TGPAAGVMTVRVAVTQKLREKIPSIAAV 232

BLAST of Csor.00g106120.m01 vs. TAIR 10
Match: AT4G25910.1 (NFU domain protein 3 )

HSP 1 Score: 94.0 bits (232), Expect = 1.6e-19
Identity = 56/139 (40.29%), Postives = 84/139 (60.43%), Query Frame = 0

Query: 74  LTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKMGIERVL 133
           LT  NV+ VL++VRP L+ADGGNV +  ++  +V LKL GACGSCPSS+ T+KMGIE  L
Sbjct: 84  LTEENVERVLDEVRPSLMADGGNVALHEIDGLVVVLKLQGACGSCPSSSMTLKMGIESRL 143

Query: 134 KEKFGDSVKEICQVYDEEP--KEITTEAVNSHLDILRPAIRNY-GGSVEVISIDGGNCVV 193
           ++K  + +  + Q  + E    E+  E +   L  LRP +    GG +E++ IDG    V
Sbjct: 144 RDKIPE-IMSVEQFLESETGGLELNDENIEKVLSELRPYLSGTGGGGLELVEIDGYVVKV 203

Query: 194 KYEGPESIGTGIKAAIKEK 210
           +  GP +    ++ A+ +K
Sbjct: 204 RLTGPAAGVMTVRVALTQK 221

BLAST of Csor.00g106120.m01 vs. TAIR 10
Match: AT5G49940.2 (NIFU-like protein 2 )

HSP 1 Score: 84.0 bits (206), Expect = 1.7e-16
Identity = 44/87 (50.57%), Postives = 58/87 (66.67%), Query Frame = 0

Query: 74  LTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKMGIERVL 133
           LT  NV+ VL+++RPYL++DGGNV +  ++  IV +KL GACGSCPSST TMKMGIER L
Sbjct: 85  LTEENVESVLDEIRPYLMSDGGNVALHEIDGNIVRVKLQGACGSCPSSTMTMKMGIERRL 144

Query: 134 KEKFGDSVKEICQVYDEEPKEITTEAV 161
            EK  + V       +E   E+  E +
Sbjct: 145 MEKIPEIVAVEALPDEETGLELNEENI 171

BLAST of Csor.00g106120.m01 vs. TAIR 10
Match: AT3G20970.1 (NFU domain protein 4 )

HSP 1 Score: 55.1 bits (131), Expect = 8.4e-08
Identity = 34/85 (40.00%), Postives = 50/85 (58.82%), Query Frame = 0

Query: 75  TTANVDLVLED-VRPYLIADGGNVDVVSV--EDGIVSLKLVGACGSCPSSTTTMKMGIER 134
           T A +  +LE  +RP +  DGG+++      E GIV L++ GAC  CPSS+ T+K GIE 
Sbjct: 192 TVAMIKELLETRIRPAVQDDGGDIEYCGFDPESGIVKLRMQGACSGCPSSSVTLKSGIEN 251

Query: 135 VLKEKFGDSVKEICQVYDEEPKEIT 157
           +L   +   VK + Q +D E +E T
Sbjct: 252 MLMH-YVSEVKGVEQEFDGEDEEGT 275

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93W776.6e-6668.72NifU-like protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU1 PE=1... [more]
Q93W208.5e-2139.19NifU-like protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU2 PE=1... [more]
Q84LK72.7e-1940.79NifU-like protein 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=N... [more]
Q84RQ72.3e-1840.29NifU-like protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU3 PE=2... [more]
O321195.0e-1354.29Putative nitrogen fixation protein YutI OS=Bacillus subtilis (strain 168) OX=224... [more]
Match NameE-valueIdentityDescription
XP_022953177.18.95e-151100.00nifU-like protein 1, chloroplastic [Cucurbita moschata] >KAG6575747.1 NifU-like ... [more]
XP_022991837.12.02e-14797.73nifU-like protein 1, chloroplastic [Cucurbita maxima][more]
XP_023547622.14.08e-14797.73nifU-like protein 1, chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_022144330.12.19e-13189.04nifU-like protein 1, chloroplastic [Momordica charantia][more]
XP_008451442.14.12e-13187.27PREDICTED: nifU-like protein 1, chloroplastic [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A6J1GMI14.33e-151100.00nifU-like protein 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111455795... [more]
A0A6J1JN019.79e-14897.73nifU-like protein 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111488364 P... [more]
A0A6J1CRB91.06e-13189.04nifU-like protein 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC11101403... [more]
A0A1S3BQX11.99e-13187.27nifU-like protein 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492737 PE=3 ... [more]
A0A5A7TT281.15e-13086.82NifU-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G... [more]
Match NameE-valueIdentityDescription
AT4G01940.14.7e-6768.72NFU domain protein 1 [more]
AT5G49940.16.0e-2239.19NIFU-like protein 2 [more]
AT4G25910.11.6e-1940.29NFU domain protein 3 [more]
AT5G49940.21.7e-1650.57NIFU-like protein 2 [more]
AT3G20970.18.4e-0840.00NFU domain protein 4 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001075NIF system FeS cluster assembly, NifU, C-terminalPFAMPF01106NifUcoord: 162..217
e-value: 3.8E-5
score: 23.8
coord: 80..139
e-value: 1.2E-22
score: 79.8
IPR034904Fe-S cluster assembly domain superfamilyGENE3D3.30.300.130coord: 155..218
e-value: 1.3E-11
score: 46.5
IPR034904Fe-S cluster assembly domain superfamilyGENE3D3.30.300.130coord: 71..154
e-value: 4.4E-27
score: 95.9
IPR034904Fe-S cluster assembly domain superfamilySUPERFAMILY117916Fe-S cluster assembly (FSCA) domain-likecoord: 77..149
IPR034904Fe-S cluster assembly domain superfamilySUPERFAMILY117916Fe-S cluster assembly (FSCA) domain-likecoord: 157..217
NoneNo IPR availablePANTHERPTHR11178:SF15NIFU-LIKE PROTEIN 1, CHLOROPLASTICcoord: 32..219
NoneNo IPR availablePANTHERPTHR11178IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATEDcoord: 32..219

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Csor.00g106120Csor.00g106120gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g106120.m01.CDS01Csor.00g106120.m01.CDS01CDS
Csor.00g106120.m01.CDS02Csor.00g106120.m01.CDS02CDS
Csor.00g106120.m01.CDS03Csor.00g106120.m01.CDS03CDS


The following stop_codon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g106120.m01.stop_codon01Csor.00g106120.m01.stop_codon01stop_codon


The following terminal feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g106120.m01.terminal01Csor.00g106120.m01.terminal01terminal


The following intron feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g106120.m01.intron01Csor.00g106120.m01.intron01intron
Csor.00g106120.m01.intron02Csor.00g106120.m01.intron02intron


The following internal feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g106120.m01.internal01Csor.00g106120.m01.internal01internal


The following initial feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g106120.m01.initial01Csor.00g106120.m01.initial01initial


The following start_codon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g106120.m01.start_codon01Csor.00g106120.m01.start_codon01start_codon


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Csor.00g106120.m01Csor.00g106120.m01-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016226 iron-sulfur cluster assembly
biological_process GO:0097428 protein maturation by iron-sulfur cluster transfer
cellular_component GO:0009507 chloroplast
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005506 iron ion binding
molecular_function GO:0051536 iron-sulfur cluster binding
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding