Csor.00g085850.m01 (mRNA) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g085850.m01
Type	mRNA
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	calmodulin-like protein 7
Location	Csor_Chr05: 10274464 .. 10275132 (+)
Sequence length	669
RNA-Seq Expression	Csor.00g085850.m01
Synteny	Csor.00g085850.m01

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSsinglestart_codonpolypeptidestop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAATCCGTGATGAACCTTCTGTTCTTGGCGGTTTTGTTCGTTGCTGGGTTTGTCAATTTCCTCATGTATTTCCCCACCAAGAGATTCACCGCTTGGTTCCAATCTATAAAACCCTCTCCCCAATCTGTCCATTTCAAACCCACCGCCGTTCCTCCGCTGTCTCCGGCGCCGGAGAAGAAGAAATCAGAGATGAAGAAGGTGTTTGGGACATTTGACAAGAACAAAGATGGGTTCATAACGAAGAAGGAGCTGATGGAATCGCTGAAGAACATGAGTATGATGATTACAGAGAGGGAAGCAGAGGAAATGGTGAAAGGGGTGGATGAAAATGGAGATGGGTTGATTGATTTTGAAGAGTTTTGTGTTTTGGGGGAGAAATTGGTGATGGGTTTTGAAGAAAAACAGAGGAGGGTTGAAGATGAAGATGAAGATGAAGATGAAGATGAACTGAGAGAGGCTTTTGGAGTGTTTGATAAGGACAAAGATGGAATGATATCAGTGGAGGAATTGGGCTTGGTGCTGTCTTGTTTGGGGATGAATGAAGGGAAGAAACTTGAGAATTGTAAGGAAATGATCAAGAAAGTGGACTTGGATGGGGATGGAATGGTGAATTTTGATGAGTTTAAGAGGATGATGAGAAGTGGAACAACTCTGGTTTTCAATTGA

mRNA sequence

ATGGAATCCGTGATGAACCTTCTGTTCTTGGCGGTTTTGTTCGTTGCTGGGTTTGTCAATTTCCTCATGTATTTCCCCACCAAGAGATTCACCGCTTGGTTCCAATCTATAAAACCCTCTCCCCAATCTGTCCATTTCAAACCCACCGCCGTTCCTCCGCTGTCTCCGGCGCCGGAGAAGAAGAAATCAGAGATGAAGAAGGTGTTTGGGACATTTGACAAGAACAAAGATGGGTTCATAACGAAGAAGGAGCTGATGGAATCGCTGAAGAACATGAGTATGATGATTACAGAGAGGGAAGCAGAGGAAATGGTGAAAGGGGTGGATGAAAATGGAGATGGGTTGATTGATTTTGAAGAGTTTTGTGTTTTGGGGGAGAAATTGGTGATGGGTTTTGAAGAAAAACAGAGGAGGGTTGAAGATGAAGATGAAGATGAAGATGAAGATGAACTGAGAGAGGCTTTTGGAGTGTTTGATAAGGACAAAGATGGAATGATATCAGTGGAGGAATTGGGCTTGGTGCTGTCTTGTTTGGGGATGAATGAAGGGAAGAAACTTGAGAATTGTAAGGAAATGATCAAGAAAGTGGACTTGGATGGGGATGGAATGGTGAATTTTGATGAGTTTAAGAGGATGATGAGAAGTGGAACAACTCTGGTTTTCAATTGA

Coding sequence (CDS)

ATGGAATCCGTGATGAACCTTCTGTTCTTGGCGGTTTTGTTCGTTGCTGGGTTTGTCAATTTCCTCATGTATTTCCCCACCAAGAGATTCACCGCTTGGTTCCAATCTATAAAACCCTCTCCCCAATCTGTCCATTTCAAACCCACCGCCGTTCCTCCGCTGTCTCCGGCGCCGGAGAAGAAGAAATCAGAGATGAAGAAGGTGTTTGGGACATTTGACAAGAACAAAGATGGGTTCATAACGAAGAAGGAGCTGATGGAATCGCTGAAGAACATGAGTATGATGATTACAGAGAGGGAAGCAGAGGAAATGGTGAAAGGGGTGGATGAAAATGGAGATGGGTTGATTGATTTTGAAGAGTTTTGTGTTTTGGGGGAGAAATTGGTGATGGGTTTTGAAGAAAAACAGAGGAGGGTTGAAGATGAAGATGAAGATGAAGATGAAGATGAACTGAGAGAGGCTTTTGGAGTGTTTGATAAGGACAAAGATGGAATGATATCAGTGGAGGAATTGGGCTTGGTGCTGTCTTGTTTGGGGATGAATGAAGGGAAGAAACTTGAGAATTGTAAGGAAATGATCAAGAAAGTGGACTTGGATGGGGATGGAATGGTGAATTTTGATGAGTTTAAGAGGATGATGAGAAGTGGAACAACTCTGGTTTTCAATTGA

Protein sequence

MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEKKKSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEFCVLGEKLVMGFEEKQRRVEDEDEDEDEDELREAFGVFDKDKDGMISVEELGLVLSCLGMNEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN

Homology

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BLAST of Csor.00g085850.m01 vs. ExPASy Swiss-Prot
Match: Q9LNE7 (Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 2.0e-33
Identity = 72/154 (46.75%), Postives = 112/154 (72.73%), Query Frame = 0


Query: 63  SEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEFC 122
           +E+K+VF  FDKN DG IT KEL E+L+++ + I ++E  +M++ +D NGDG +D +EF 
Sbjct: 4   TELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEFG 63

Query: 123 VLGEKLVMGFEEKQRRVEDEDEDEDEDELREAFGVFDKDKDGMISVEELGLVLSCLGMNE 182
            L + ++            ++EDE+E++++EAF VFD++ DG I+V+EL  VLS LG+ +
Sbjct: 64  ELYKTIM------------DEEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQ 123

Query: 183 GKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSG 217
           GK L++CK+MIKKVD+DGDG VN+ EF++MM+ G
Sbjct: 124 GKTLDDCKKMIKKVDVDGDGRVNYKEFRQMMKGG 145

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BLAST of Csor.00g085850.m01 vs. ExPASy Swiss-Prot
Match: Q9SRR7 (Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 7.4e-33
Identity = 74/155 (47.74%), Postives = 111/155 (71.61%), Query Frame = 0


Query: 62  KSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEF 121
           ++E+ ++F  FD+N DG ITK+EL +SL+N+ + I +++  +M++ +D NGDG +D EEF
Sbjct: 3   QAELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEF 62

Query: 122 CVLGEKLVMGFEEKQRRVEDEDEDEDEDELREAFGVFDKDKDGMISVEELGLVLSCLGMN 181
             L + ++            E+ DE+ED +REAF VFD+++DG I+VEEL  VL+ LG+ 
Sbjct: 63  GGLYQTIM------------EERDEEED-MREAFNVFDQNRDGFITVEELRSVLASLGLK 122

Query: 182 EGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSG 217
           +G+ LE+CK MI KVD+DGDGMVNF EFK+MM+ G
Sbjct: 123 QGRTLEDCKRMISKVDVDGDGMVNFKEFKQMMKGG 144

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BLAST of Csor.00g085850.m01 vs. ExPASy Swiss-Prot
Match: Q9SU00 (Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 8.2e-32
Identity = 73/155 (47.10%), Postives = 105/155 (67.74%), Query Frame = 0


Query: 62  KSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEF 121
           + E+ +VF  FDKN DG I K EL +  K++ +M+ E E  EM+  +D NGDG +D +EF
Sbjct: 3   RGELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEF 62

Query: 122 CVLGEKLVMGFEEKQRRVEDEDEDEDEDELREAFGVFDKDKDGMISVEELGLVLSCLGMN 181
             L +++V             +E E+E+++REAF VFD++ DG I+ EEL  VL+ +G+ 
Sbjct: 63  GSLYQEMV-------------EEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLK 122

Query: 182 EGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSG 217
           +G+ LE+CK+MI KVD+DGDGMVNF EFK+MMR G
Sbjct: 123 QGRTLEDCKKMISKVDVDGDGMVNFKEFKQMMRGG 144

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BLAST of Csor.00g085850.m01 vs. ExPASy Swiss-Prot
Match: Q9ZR02 (Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 1.8e-31
Identity = 75/154 (48.70%), Postives = 108/154 (70.13%), Query Frame = 0


Query: 63  SEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEFC 122
           +E+ +VF  FDK+ DG IT KEL ES KN+ ++I E E  ++++ +D NGDG +D EEF 
Sbjct: 4   TELNRVFQMFDKDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIEEFG 63

Query: 123 VLGEKLVMGFEEKQRRVEDEDEDEDEDELREAFGVFDKDKDGMISVEELGLVLSCLGMNE 182
            L + ++         VEDEDE  +ED ++EAF VFD++ DG I+V+EL  VLS LG+ +
Sbjct: 64  ELYKTIM---------VEDEDEVGEED-MKEAFNVFDRNGDGFITVDELKAVLSSLGLKQ 123

Query: 183 GKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSG 217
           GK LE C++MI +VD+DGDG VN+ EF++MM+ G
Sbjct: 124 GKTLEECRKMIMQVDVDGDGRVNYMEFRQMMKKG 147

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BLAST of Csor.00g085850.m01 vs. ExPASy Swiss-Prot
Match: O22845 (Calmodulin-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=CML5 PE=2 SV=2)

HSP 1 Score: 135.2 bits (339), Expect = 9.1e-31
Identity = 79/192 (41.15%), Postives = 123/192 (64.06%), Query Frame = 0


Query: 32  AWF-----QSIKPSPQSVHFKPTAVPPLSPAPEKK--KSEMKKVFGTFDKNKDGFITKKE 91
           +WF     ++  PSP ++   P++    S AP K+   SE+K+VF  FDKN DG ITK+E
Sbjct: 30  SWFDKTLHKNSPPSPSTMLPSPSS----SSAPTKRIDPSELKRVFQMFDKNGDGRITKEE 89

Query: 92  LMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEFCVLGEKLVMGFEEKQRRVEDEDE 151
           L +SL+N+ + I +++  +M+  +D NGDG +D +EF    E L     ++       D 
Sbjct: 90  LNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDIDEF----ESLYSSIVDEHH----NDG 149

Query: 152 DEDEDELREAFGVFDKDKDGMISVEELGLVLSCLGMNEGKKLENCKEMIKKVDLDGDGMV 211
           + +E+++++AF VFD+D DG I+VEEL  V++ LG+ +GK L+ CK+MI +VD DGDG V
Sbjct: 150 ETEEEDMKDAFNVFDQDGDGFITVEELKSVMASLGLKQGKTLDGCKKMIMQVDADGDGRV 209

Query: 212 NFDEFKRMMRSG 217
           N+ EF +MM+ G
Sbjct: 210 NYKEFLQMMKGG 209

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BLAST of Csor.00g085850.m01 vs. NCBI nr
Match: KAG6599380.1 (Calmodulin-like protein 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 428 bits (1101), Expect = 5.17e-151
Identity = 222/222 (100.00%), Postives = 222/222 (100.00%), Query Frame = 0


Query: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEK 60
           MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEK
Sbjct: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEK 60

Query: 61  KKSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 120
           KKSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE
Sbjct: 61  KKSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 120

Query: 121 FCVLGEKLVMGFEEKQRRVEDEDEDEDEDELREAFGVFDKDKDGMISVEELGLVLSCLGM 180
           FCVLGEKLVMGFEEKQRRVEDEDEDEDEDELREAFGVFDKDKDGMISVEELGLVLSCLGM
Sbjct: 121 FCVLGEKLVMGFEEKQRRVEDEDEDEDEDELREAFGVFDKDKDGMISVEELGLVLSCLGM 180

Query: 181 NEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN 222
           NEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN
Sbjct: 181 NEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN 222

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BLAST of Csor.00g085850.m01 vs. NCBI nr
Match: XP_022946838.1 (calmodulin-like protein 7 [Cucurbita moschata])

HSP 1 Score: 427 bits (1097), Expect = 2.10e-150
Identity = 221/222 (99.55%), Postives = 222/222 (100.00%), Query Frame = 0


Query: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEK 60
           MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEK
Sbjct: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEK 60

Query: 61  KKSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 120
           KKSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE
Sbjct: 61  KKSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 120

Query: 121 FCVLGEKLVMGFEEKQRRVEDEDEDEDEDELREAFGVFDKDKDGMISVEELGLVLSCLGM 180
           FCVLGEKLVMGFEEKQRRVEDEDEDEDEDELREAFGVFDKDKDG+ISVEELGLVLSCLGM
Sbjct: 121 FCVLGEKLVMGFEEKQRRVEDEDEDEDEDELREAFGVFDKDKDGVISVEELGLVLSCLGM 180

Query: 181 NEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN 222
           NEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN
Sbjct: 181 NEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN 222

[Close]

BLAST of Csor.00g085850.m01 vs. NCBI nr
Match: XP_023545557.1 (calmodulin-like protein 7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 414 bits (1064), Expect = 2.10e-145
Identity = 217/222 (97.75%), Postives = 218/222 (98.20%), Query Frame = 0


Query: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEK 60
           MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEK
Sbjct: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEK 60

Query: 61  KKSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 120
           KKSEMKKVFGTFDKN DGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE
Sbjct: 61  KKSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 120

Query: 121 FCVLGEKLVMGFEEKQRRVEDEDEDEDEDELREAFGVFDKDKDGMISVEELGLVLSCLGM 180
           FCVLGEKLVMGFE KQRRVEDEDEDEDE  LREAFGVFDKDKDG+ISVEELGLVLSCLGM
Sbjct: 121 FCVLGEKLVMGFEVKQRRVEDEDEDEDE--LREAFGVFDKDKDGVISVEELGLVLSCLGM 180

Query: 181 NEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN 222
           NEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN
Sbjct: 181 NEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN 220

[Close]

BLAST of Csor.00g085850.m01 vs. NCBI nr
Match: XP_022999398.1 (calmodulin-like protein 7 [Cucurbita maxima])

HSP 1 Score: 408 bits (1048), Expect = 5.38e-143
Identity = 213/222 (95.95%), Postives = 216/222 (97.30%), Query Frame = 0


Query: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEK 60
           MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPP SPAPEK
Sbjct: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPPSPAPEK 60

Query: 61  KKSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 120
           KKSEMKKVFGTFDKN DGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE
Sbjct: 61  KKSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 120

Query: 121 FCVLGEKLVMGFEEKQRRVEDEDEDEDEDELREAFGVFDKDKDGMISVEELGLVLSCLGM 180
           FCVLGEKLV+GFEEKQRR+EDEDEDE    LREAFGVFDKDKDG+ISVEELGLVLSCLGM
Sbjct: 121 FCVLGEKLVVGFEEKQRRIEDEDEDE----LREAFGVFDKDKDGVISVEELGLVLSCLGM 180

Query: 181 NEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN 222
           NEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN
Sbjct: 181 NEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN 218

[Close]

BLAST of Csor.00g085850.m01 vs. NCBI nr
Match: XP_038889113.1 (calmodulin-like protein 3 [Benincasa hispida])

HSP 1 Score: 330 bits (847), Expect = 2.70e-112
Identity = 178/222 (80.18%), Postives = 192/222 (86.49%), Query Frame = 0


Query: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAV-PPLSPAPE 60
           ME +MN L L VL VAGFVNFL+YFPTKRF+AWFQSIKPSPQ  HFK  ++ PP  P P 
Sbjct: 1   MEPIMNFLLLVVLVVAGFVNFLLYFPTKRFSAWFQSIKPSPQISHFKSLSLQPPPPPPPS 60

Query: 61  KKKSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFE 120
               E+KKVFGTFDKN DGFITKKELMESLKNM M+ITE++AEEM+KGVDENGDGLIDFE
Sbjct: 61  PPSPELKKVFGTFDKNNDGFITKKELMESLKNMRMLITEKDAEEMLKGVDENGDGLIDFE 120

Query: 121 EFCVLGEKLVMGFEEKQRR-VEDEDEDEDEDELREAFGVFDKDKDGMISVEELGLVLSCL 180
           EFCVLGEKLVMGF EK++  VEDE   EDEDELREAFGVFDKDKDGMISVEEL LVL  L
Sbjct: 121 EFCVLGEKLVMGFGEKKKICVEDE---EDEDELREAFGVFDKDKDGMISVEELSLVLCSL 180

Query: 181 GMNEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLV 220
           GMNEGKK+ENCKEMIKKVDLDGDGMVNFDEFKRMMRSG +LV
Sbjct: 181 GMNEGKKVENCKEMIKKVDLDGDGMVNFDEFKRMMRSGVSLV 219

[Close]

BLAST of Csor.00g085850.m01 vs. ExPASy TrEMBL
Match: A0A6J1G4Z1 (calmodulin-like protein 7 OS=Cucurbita moschata OX=3662 GN=LOC111450782 PE=4 SV=1)

HSP 1 Score: 427 bits (1097), Expect = 1.02e-150
Identity = 221/222 (99.55%), Postives = 222/222 (100.00%), Query Frame = 0


Query: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEK 60
           MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEK
Sbjct: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEK 60

Query: 61  KKSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 120
           KKSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE
Sbjct: 61  KKSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 120

Query: 121 FCVLGEKLVMGFEEKQRRVEDEDEDEDEDELREAFGVFDKDKDGMISVEELGLVLSCLGM 180
           FCVLGEKLVMGFEEKQRRVEDEDEDEDEDELREAFGVFDKDKDG+ISVEELGLVLSCLGM
Sbjct: 121 FCVLGEKLVMGFEEKQRRVEDEDEDEDEDELREAFGVFDKDKDGVISVEELGLVLSCLGM 180

Query: 181 NEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN 222
           NEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN
Sbjct: 181 NEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN 222

[Close]

BLAST of Csor.00g085850.m01 vs. ExPASy TrEMBL
Match: A0A6J1KJL5 (calmodulin-like protein 7 OS=Cucurbita maxima OX=3661 GN=LOC111493780 PE=4 SV=1)

HSP 1 Score: 408 bits (1048), Expect = 2.60e-143
Identity = 213/222 (95.95%), Postives = 216/222 (97.30%), Query Frame = 0


Query: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEK 60
           MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPP SPAPEK
Sbjct: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPPSPAPEK 60

Query: 61  KKSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 120
           KKSEMKKVFGTFDKN DGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE
Sbjct: 61  KKSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 120

Query: 121 FCVLGEKLVMGFEEKQRRVEDEDEDEDEDELREAFGVFDKDKDGMISVEELGLVLSCLGM 180
           FCVLGEKLV+GFEEKQRR+EDEDEDE    LREAFGVFDKDKDG+ISVEELGLVLSCLGM
Sbjct: 121 FCVLGEKLVVGFEEKQRRIEDEDEDE----LREAFGVFDKDKDGVISVEELGLVLSCLGM 180

Query: 181 NEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN 222
           NEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN
Sbjct: 181 NEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN 218

[Close]

BLAST of Csor.00g085850.m01 vs. ExPASy TrEMBL
Match: A0A0A0LL19 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G007990 PE=4 SV=1)

HSP 1 Score: 306 bits (785), Expect = 4.10e-103
Identity = 166/229 (72.49%), Postives = 189/229 (82.53%), Query Frame = 0


Query: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAV---PPLSPA 60
           ME + N L L+VLFVAGF+NFL+YFP+KRF+AWFQSIKPS Q  HFK T +   PP SP+
Sbjct: 1   MEPIFNFLLLSVLFVAGFINFLLYFPSKRFSAWFQSIKPSSQITHFKSTPLQPPPPPSPS 60

Query: 61  PEKKKS-----EMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENG 120
           P          EMKKVFGTFDKN DGFITKKELMESLK+M MMITE++AEEM+KGVDENG
Sbjct: 61  PSPSPPPPSAMEMKKVFGTFDKNDDGFITKKELMESLKSMRMMITEKDAEEMLKGVDENG 120

Query: 121 DGLIDFEEFCVLGEKLVMGFEE-KQRRVEDEDEDEDEDELREAFGVFDKDKDGMISVEEL 180
           DGLIDFEEFCVLG KL+MGFEE K+  VEDE     EDEL++AFGVFDKD DG+ISVEEL
Sbjct: 121 DGLIDFEEFCVLGGKLMMGFEENKKTSVEDE-----EDELKDAFGVFDKDSDGLISVEEL 180

Query: 181 GLVLSCLGMNEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLV 220
            LVL  LGMNEGK +ENCKEMI+KVDLDGDGMVNFDEFK+MMR+G T++
Sbjct: 181 SLVLCSLGMNEGKIVENCKEMIRKVDLDGDGMVNFDEFKKMMRNGVTIL 224

[Close]

BLAST of Csor.00g085850.m01 vs. ExPASy TrEMBL
Match: A0A1S3C5X4 (calmodulin-like protein 7 OS=Cucumis melo OX=3656 GN=LOC103496824 PE=4 SV=1)

HSP 1 Score: 301 bits (772), Expect = 3.64e-101
Identity = 160/226 (70.80%), Postives = 184/226 (81.42%), Query Frame = 0


Query: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAV------PPL 60
           ME + N L L+VLFVAGFVNFL+YFPTKRFTAWFQSIKPS Q  HFK T +      PP 
Sbjct: 1   MEPIFNFLLLSVLFVAGFVNFLLYFPTKRFTAWFQSIKPSSQIPHFKSTPLQPPPPPPPP 60

Query: 61  SPAPEKKKSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDG 120
            P P     E+KKVFGTFDKN DGFITKKELMESLK+M MMITE++AEEM+K VDENGDG
Sbjct: 61  PPPPPPSAMELKKVFGTFDKNDDGFITKKELMESLKSMRMMITEKDAEEMLKEVDENGDG 120

Query: 121 LIDFEEFCVLGEKLVMGFEEKQRRVEDEDEDEDEDELREAFGVFDKDKDGMISVEELGLV 180
           LIDFEEFCVLGEKL+MGFEE ++    +DE+     L++AFGVFDKD DG+ISVEEL LV
Sbjct: 121 LIDFEEFCVLGEKLLMGFEENKKTSVGDDEEG----LKDAFGVFDKDSDGLISVEELSLV 180

Query: 181 LSCLGMNEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLV 220
           L  LGMNEGK +ENCKEMI+KVDLDGDGMVNFDEFK+MMR+G +++
Sbjct: 181 LCSLGMNEGKIVENCKEMIRKVDLDGDGMVNFDEFKKMMRNGVSIL 222

[Close]

BLAST of Csor.00g085850.m01 vs. ExPASy TrEMBL
Match: A0A6J1CMI5 (calmodulin-like protein 7 OS=Momordica charantia OX=3673 GN=LOC111012994 PE=4 SV=1)

HSP 1 Score: 260 bits (664), Expect = 4.52e-84
Identity = 144/217 (66.36%), Postives = 175/217 (80.65%), Query Frame = 0


Query: 2   ESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEKK 61
           +S+MN+L LAVLFVAG VNFL+YFPTKRF+AW   +K SP     KP AV   + AP + 
Sbjct: 67  QSMMNILLLAVLFVAGLVNFLVYFPTKRFSAW---LKQSPT----KPAAVA-AAVAPPQP 126

Query: 62  KSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEF 121
            +E++K+F TFDKN DG+IT+KEL ESL+N    I EREAEE+ +GVDENGDGLIDFEEF
Sbjct: 127 PTELRKIFRTFDKNNDGYITRKELTESLRNW---IAEREAEELFEGVDENGDGLIDFEEF 186

Query: 122 CVLGEKLVMGFEEKQRRVEDEDEDEDEDELREAFGVFDKDKDGMISVEELGLVLSCLGMN 181
           CVL EKL        R+ ++E+E++D++ELREAFGVFDKDKDG+ISVEELGLVLSCLG+ 
Sbjct: 187 CVLAEKL--------RKEKEEEEEDDDEELREAFGVFDKDKDGVISVEELGLVLSCLGLK 246

Query: 182 EGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTT 218
           EGK  ENC+EMI+KVDLDGDGMVNF+EFKRMMR+G T
Sbjct: 247 EGKMAENCREMIRKVDLDGDGMVNFEEFKRMMRNGGT 264

[Close]

BLAST of Csor.00g085850.m01 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 144.1 bits (362), Expect = 1.4e-34
Identity = 72/154 (46.75%), Postives = 112/154 (72.73%), Query Frame = 0


Query: 63  SEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEFC 122
           +E+K+VF  FDKN DG IT KEL E+L+++ + I ++E  +M++ +D NGDG +D +EF 
Sbjct: 4   TELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEFG 63

Query: 123 VLGEKLVMGFEEKQRRVEDEDEDEDEDELREAFGVFDKDKDGMISVEELGLVLSCLGMNE 182
            L + ++            ++EDE+E++++EAF VFD++ DG I+V+EL  VLS LG+ +
Sbjct: 64  ELYKTIM------------DEEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQ 123

Query: 183 GKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSG 217
           GK L++CK+MIKKVD+DGDG VN+ EF++MM+ G
Sbjct: 124 GKTLDDCKKMIKKVDVDGDGRVNYKEFRQMMKGG 145

[Close]

BLAST of Csor.00g085850.m01 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 142.1 bits (357), Expect = 5.3e-34
Identity = 74/155 (47.74%), Postives = 111/155 (71.61%), Query Frame = 0


Query: 62  KSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEF 121
           ++E+ ++F  FD+N DG ITK+EL +SL+N+ + I +++  +M++ +D NGDG +D EEF
Sbjct: 3   QAELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEF 62

Query: 122 CVLGEKLVMGFEEKQRRVEDEDEDEDEDELREAFGVFDKDKDGMISVEELGLVLSCLGMN 181
             L + ++            E+ DE+ED +REAF VFD+++DG I+VEEL  VL+ LG+ 
Sbjct: 63  GGLYQTIM------------EERDEEED-MREAFNVFDQNRDGFITVEELRSVLASLGLK 122

Query: 182 EGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSG 217
           +G+ LE+CK MI KVD+DGDGMVNF EFK+MM+ G
Sbjct: 123 QGRTLEDCKRMISKVDVDGDGMVNFKEFKQMMKGG 144

[Close]

BLAST of Csor.00g085850.m01 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 138.7 bits (348), Expect = 5.8e-33
Identity = 73/155 (47.10%), Postives = 105/155 (67.74%), Query Frame = 0


Query: 62  KSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEF 121
           + E+ +VF  FDKN DG I K EL +  K++ +M+ E E  EM+  +D NGDG +D +EF
Sbjct: 3   RGELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEF 62

Query: 122 CVLGEKLVMGFEEKQRRVEDEDEDEDEDELREAFGVFDKDKDGMISVEELGLVLSCLGMN 181
             L +++V             +E E+E+++REAF VFD++ DG I+ EEL  VL+ +G+ 
Sbjct: 63  GSLYQEMV-------------EEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLK 122

Query: 182 EGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSG 217
           +G+ LE+CK+MI KVD+DGDGMVNF EFK+MMR G
Sbjct: 123 QGRTLEDCKKMISKVDVDGDGMVNFKEFKQMMRGG 144

[Close]

BLAST of Csor.00g085850.m01 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 137.5 bits (345), Expect = 1.3e-32
Identity = 75/154 (48.70%), Postives = 108/154 (70.13%), Query Frame = 0


Query: 63  SEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEFC 122
           +E+ +VF  FDK+ DG IT KEL ES KN+ ++I E E  ++++ +D NGDG +D EEF 
Sbjct: 4   TELNRVFQMFDKDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIEEFG 63

Query: 123 VLGEKLVMGFEEKQRRVEDEDEDEDEDELREAFGVFDKDKDGMISVEELGLVLSCLGMNE 182
            L + ++         VEDEDE  +ED ++EAF VFD++ DG I+V+EL  VLS LG+ +
Sbjct: 64  ELYKTIM---------VEDEDEVGEED-MKEAFNVFDRNGDGFITVDELKAVLSSLGLKQ 123

Query: 183 GKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSG 217
           GK LE C++MI +VD+DGDG VN+ EF++MM+ G
Sbjct: 124 GKTLEECRKMIMQVDVDGDGRVNYMEFRQMMKKG 147

[Close]

BLAST of Csor.00g085850.m01 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 135.2 bits (339), Expect = 6.5e-32
Identity = 79/192 (41.15%), Postives = 123/192 (64.06%), Query Frame = 0


Query: 32  AWF-----QSIKPSPQSVHFKPTAVPPLSPAPEKK--KSEMKKVFGTFDKNKDGFITKKE 91
           +WF     ++  PSP ++   P++    S AP K+   SE+K+VF  FDKN DG ITK+E
Sbjct: 30  SWFDKTLHKNSPPSPSTMLPSPSS----SSAPTKRIDPSELKRVFQMFDKNGDGRITKEE 89

Query: 92  LMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEFCVLGEKLVMGFEEKQRRVEDEDE 151
           L +SL+N+ + I +++  +M+  +D NGDG +D +EF    E L     ++       D 
Sbjct: 90  LNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDIDEF----ESLYSSIVDEHH----NDG 149

Query: 152 DEDEDELREAFGVFDKDKDGMISVEELGLVLSCLGMNEGKKLENCKEMIKKVDLDGDGMV 211
           + +E+++++AF VFD+D DG I+VEEL  V++ LG+ +GK L+ CK+MI +VD DGDG V
Sbjct: 150 ETEEEDMKDAFNVFDQDGDGFITVEELKSVMASLGLKQGKTLDGCKKMIMQVDADGDGRV 209

Query: 212 NFDEFKRMMRSG 217
           N+ EF +MM+ G
Sbjct: 210 NYKEFLQMMKGG 209

The following BLAST results are available for this feature:

BLAST of Csor.00g085850.m01 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LNE7	2.0e-33	46.75	Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1	[more]
Q9SRR7	7.4e-33	47.74	Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1	[more]
Q9SU00	8.2e-32	47.10	Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1	[more]
Q9ZR02	1.8e-31	48.70	Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1	[more]
O22845	9.1e-31	41.15	Calmodulin-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=CML5 PE=2 SV=2	[more]

BLAST of Csor.00g085850.m01 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6599380.1	5.17e-151	100.00	Calmodulin-like protein 4, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022946838.1	2.10e-150	99.55	calmodulin-like protein 7 [Cucurbita moschata]	[more]
XP_023545557.1	2.10e-145	97.75	calmodulin-like protein 7 [Cucurbita pepo subsp. pepo]	[more]
XP_022999398.1	5.38e-143	95.95	calmodulin-like protein 7 [Cucurbita maxima]	[more]
XP_038889113.1	2.70e-112	80.18	calmodulin-like protein 3 [Benincasa hispida]	[more]

BLAST of Csor.00g085850.m01 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G4Z1	1.02e-150	99.55	calmodulin-like protein 7 OS=Cucurbita moschata OX=3662 GN=LOC111450782 PE=4 SV=...	[more]
A0A6J1KJL5	2.60e-143	95.95	calmodulin-like protein 7 OS=Cucurbita maxima OX=3661 GN=LOC111493780 PE=4 SV=1	[more]
A0A0A0LL19	4.10e-103	72.49	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G007990 PE=4 SV=1	[more]
A0A1S3C5X4	3.64e-101	70.80	calmodulin-like protein 7 OS=Cucumis melo OX=3656 GN=LOC103496824 PE=4 SV=1	[more]
A0A6J1CMI5	4.52e-84	66.36	calmodulin-like protein 7 OS=Momordica charantia OX=3673 GN=LOC111012994 PE=4 SV...	[more]

BLAST of Csor.00g085850.m01 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G05990.1	1.4e-34	46.75	EF hand calcium-binding protein family	[more]
AT3G07490.1	5.3e-34	47.74	ARF-GAP domain 11	[more]
AT4G12860.1	5.8e-33	47.10	EF hand calcium-binding protein family	[more]
AT4G03290.1	1.3e-32	48.70	EF hand calcium-binding protein family	[more]
AT2G43290.1	6.5e-32	41.15	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 150..178 e-value: 1.7E-5 score: 34.3 coord: 64..92 e-value: 2.6E-4 score: 30.3 coord: 100..128 e-value: 0.0058 score: 25.9 coord: 188..216 e-value: 5.2E-6 score: 36.0
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 149..214 e-value: 1.3E-11 score: 44.8 coord: 63..122 e-value: 1.2E-11 score: 44.9
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 184..219 score: 14.401823
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 96..131 score: 11.723866
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 146..181 score: 15.88028
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 60..95 score: 13.537066
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 64..122 e-value: 3.65748E-15 score: 65.6469
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 151..214 e-value: 1.73083E-17 score: 71.8101
None	No IPR available	GENE3D	1.10.238.10		coord: 147..216 e-value: 7.0E-22 score: 79.5
None	No IPR available	GENE3D	1.10.238.10		coord: 29..124 e-value: 2.8E-20 score: 74.4
None	No IPR available	PANTHER	PTHR10891:SF796	CALCIUM-BINDING PROTEIN CML30-RELATED	coord: 5..220
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 5..220
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 159..171
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 197..209
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 109..121
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 73..85
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 59..216

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Csor.00g085850	Csor.00g085850	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csor.00g085850.m01.CDS01	Csor.00g085850.m01.CDS01	CDS

The following single feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csor.00g085850.m01.single01	Csor.00g085850.m01.single01	single

The following start_codon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csor.00g085850.m01.start_codon01	Csor.00g085850.m01.start_codon01	start_codon

The following stop_codon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csor.00g085850.m01.stop_codon01	Csor.00g085850.m01.stop_codon01	stop_codon

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Csor.00g085850.m01	Csor.00g085850.m01-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005509	calcium ion binding