Csor.00g010220.m01 (mRNA) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g010220.m01
Type	mRNA
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	beta-amyrin 11-oxidase-like
Location	Csor_Chr16: 7875627 .. 7880307 (-)
Sequence length	771
RNA-Seq Expression	Csor.00g010220.m01
Synteny	Csor.00g010220.m01

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAAGGTTATTTGGTAGAAAGTCACTGATCCGAGTCTCCAAGGCGGAGCACCAGAAGCTCCGACGGATGACAATGGCGCCGATCAGCGGGCATGCTGCGCTCGAGATGTACATCGATCATATTGAACACACAGTGATCAGTGGGTTGGAGGAATGGTCAAGCATGGAGAAGCCATTGAAGCTGTTGACAGAGATAAAAGAGCTTACCTTCAAACTTATTTGGAACATTTTTATGGGTTCAACTTCCATAGACTATACCACAAAAGAAATGGAGGCTTTGTATGATGATGTTGCACTTGGCTTATTCTGTTTCCCCATCAACTTTCCTGGCTTCAACTTCCATAAATCTCTCAAGGTAACCACTTTCTTTTCCTTCTAAATATGTATATTAGACGAGAATGTCACGGTTCAATCAGCTAAAATATTATACTATCTAGTACATGTTATAGATTCATATCTGAATAGTATATGTCTTACCCTGAAAAGTATCTTCTAACCTATGATATTTAGGTGATCCGTTTATGAATTGCAACTTTTGAAAAAATTTAATTATTTTTCAAAGCAAATCTTTTATCATGCAACATATAATGTAATAGCTCAAGCTTACCGCTAGTAGATATTATCTGCTTTGACTAGTTACGTATCGTCAGTTTCAGGGTTTTAAAGCGCGTATGCTAGGGAGAGGTTTCCACACCCTTATAAGAAATGTTTCGTTCCCCTCTCCAACTGATGTGGAATCTCATAATCCACTCCCTTGGAGACCAGTGTCCTCGTTGGGCTTTGATACCATTTGTAACCACTCAAGCTCACCACTAGATATTGTCCGCTTTAGCCCGTTACATATCATCGTCAACCTCACGATTTTAAAGTCCGTCTGCTAGAGAGAGGTTTTCACACCCTTATAAGGAATGTTTTGTTCCCCTCTTCAACCGATGTAGGATCTCACATATAAATTTTCCATCAATCCCATATATGATCGATTAACGAAAATAAATGTATGAATACAGGCTCGAAAAAGATTGTATGAAATACTTCTATCTATTGTGAATGAGAAGAGGTTAATGAAGAAAAGCAAAGGGAAAGTTGGGAAGCAAAAGATATGATGGATTTGATGATAGAAGTGGAAGATGAAGATGGTGAAGGGATGGACAATGAGACCATTACAGATTTGATTTTTGGTAAGTTATTTGCAGGCCATGAGACTTCAGCTTTCACTACAATGTGGGCAATTATATTTCTTACAGATCATCCACATATATTTCAAAGGGCCAAGGTTAGAGTTCTTTAATTTTCTAACTTGTTAACTGCATTCATTTGTCTAAATCTTATGATCTCTTTATTTTTCTTAGTTATTGCGTATTAATTTTGTCTCGAATACGTTTTTGGTCTCTTTAATTTTGTGATACAAGAATATTTGGCTCGTAGGCTAGAGTTGTGTAGTCTAAGGTTAGGAAATATACTTGTTTATCCAAAGTTATGGGGTTCACGACGACATGAATGTGATGTTCGAGTAAAAGAACAATATATGAATGAACCGAGACCATATCTAAATGATAAAAATTTCGATGACTAAATCATAAAAATTTCGTTAAACATGTTTGACCTAGAATGTAATAGCTCAATCTCATCGCTAGCAGATATTATCCTCTTTAGGCTTTTCCTTTCGGGATTCCCCTCAAGGTTTTTAAAACGCGTCTGCTAAGGAGAGGTTTTTCACACTCTTATAAAGTGTGTTTCGTTCTACTCCCCAAACAATGTGGGATCTCACAATCCATCCCCGTTCAGAGCCTAGCCTCCTTCTTGGCACTCATTCCCTTTCTCAATCGATTTGGGACCCCCCCCCCCCCCCCCCCAATCCACCCCTTTTGGGGCCAGCGTCCTTGTTGGCACACCGCCTCGTGTCCACCCTCTTCGGGGCTCAACCTCCTCGCTTTTCCATCACCCGGTGTCTGGCTCTAATACCATTTGTAACAGCCAAAGCTCATTGCTACTAGATATTGTCCTCTTTGTGGTTTCCCTTTTGGGCTTCCCCTCGAGGTTTTTAAAACGCGTCTATTAGAAAAAGGTTTCCACACCCTTATAAATAATGTTTAGTTCTCCTCCCGAACCGATGTGGGATCTCACAATATCATGGCAACAAAGTGGTTGGAGGTTGCCTCGGCCCATTGGTTAGGGTTGCTAGAGCAGTTCTCAGGCCTATCCATACCAAGTAGGGAGTTTGACCATTAGAAATGGTATCAGAGACAAATACTAGACGATGTACCAACGAGGAGATTGAGCCCTGAAGGGGGTGGACACAAGGCGGTGTGCCAGCAAGGATGTTGGATTCTGAAGGGGGTGGATTGAGTTGTCTCACATCGATTGGAGAAGGGAACGAGTGCCAGAGAAGACGTTGAGCCCCGAAGGGAGTGGACTGTGAGATCCCACATATGTTGAGGAGGAAATGAAACATTTTTTTATAAGGGTGTAAAAACCTCTTCTTAGCATAGCAGACGCTATTTAGGGAAAGCCAAAAGAGGACAATATTTGTTAGCGGTGCGCTTGGACTGTTATATGAAACAAACAAGTTACAAGACTTGTGTAGAAACTATTATACTCTACTGTGTTTTAGATTTTTTAGATGAAAATCTTACCCAGTTGTACACGCTCTTTATTAAGACCAATCATTAAAAATTACATGATAGAACACTTACACTATTTATTAAGACCAATCAATAAGAGATTACAACACTCTGTATAGAAACTATTATACTTAGTGTTTTGAATTTTTTGGATGAAATTCTTAGGGAGAGGTATAGGAGAGACTAGACAATTTATTCATAAAATATTTAAATATTTATTCAAAAAAATGTCTAAACCATTTTCTTAAAATAATTAGATATTTTTGTTCCTAAAATACCAATATTTTTCTTAAAATATATACACCTTCCTACAACTATGAGACTTGACTTACTAGGCGAGTGATATATTTTAACACTAATCTCTTGAAACTTTTACTACTAATATGTGCGTGAGGACAAAGCTTGCTAAAAAGTTTCAAGATAATTAGGAAATGTGGCTATGTCGTAGGAAATGCAAATAAATGTCGGGTCATCAATTAGGCCTCATTTCTTACAATAAACTTATCTAATCGGTAATAAATAAGAATTTTATTTTCTAATTTTGTTTCTAAAAATTAATTAAGCTACATGTTTTAGAATAAGAAACAAATAAGAGGCTAGAATTCGTTCATGCATATGGATTTGTGTTCATATAAATCTTAATCCCTTCGAAAAGCCGAGTGAAATTTAAGATATTGCTCTTTCTAATATTATTTTGGATGATTTTATCATTTTGATGGAAATTTTTAAGATTAATTTGTTTTAGGAAGAACAAGAAGATATCATTAGGCGAAGACCTTCAACACAAAAGGGTATAAATCTCGTCGAGTTTAAACAAATGAAATTTCTTTCTCTATATATTTTTATCTTCAATGTTGGCTAAGATGTTTAAATAAACTACATATCTAAAACATTGTTAATAACGAAATTCTGTATAATTGTAATATCTAGGTGATAGATGAGACGCTTCGTCTTAGTAGTATTGCATTTGCTACTTTTCGTGAGGCAACAACTGATATTAATATCAATGGTTAGTCATCAATGGAGAAAGCTCGGGTTATACGATATCCTTATTTTGATTGATCTCAATCCCGTTGTGCTAAAGAAGGTGAAATTGTATACTAAGCTTGCATTTTCATTTTTTAGGTAAACTTATACCAAAAGGATGGAAAGTTATACTTTGGCATCGAGGGCTGTACATGGATGAAGAGCTACATCCTTCCCCACAAGACTTCAATCCATCGAGATGGGATGTATGCAATCATTTTCCATATTTGCTATCCTTTGTTATTGATTTTGTTCTACTTAAAATCATATTCTACATGCAATAAATACATGAAATATAAATGGACTAAACAATAAATACATGCAATACTTCTAATTTTGTAGTTTAGTTTTCATTTGGTAGGTTAGAAATTACGAATTTTAAGTATAAGCTTTTATAACCTTGTTCTTAATTTCTCCGAGAGGTCTCATATCAGTGGAGATAATGTTCCTTACTCAAAATGTATTTTTTTCTCGCATGTTATATCAGTTGAGTGATTGTTTATAATCATTAATGTTATTTCTTATCATTTGATTATTTATGTTGAACAGGATTTTATAGGCAACCCCGGTGCTTTTACTCCGTTTGGATTAGGAGTCAGGTTGTGCCCTGGACGTGATCTTGCCAAGCTCGAGATCTCAATTTTCCTTCACCATTTTCTCCTCAATTACAAGTGAGTTTTCTCCAACTTTTCTTCTATAAACTTCTAACACAATTAAATATAAGCTTTTAAAAGATGTAGTTGGTGACATTTTTATGTTCCTGGTTCCATTTTTCATATAGAAAAATGTTTTGAAAAAATGAATAACAAGAACTATGAATAGTAACTATATGATTCTCAAGTTCATGATTCAAGAAATACACATTATCAAACATACCCTAATTTTTCACAATGTATTGTTAATATATACGTTTTTGGTGAACGTAGGATCCAGCGATATAATCCACAATGCCAATTGACTTATCTACCACTTCCTCATCCCAAAGACAAATGCCTTGCAAGAGTGCTGAAAGCTGCAGGAGAATGTGTATAA

mRNA sequence

ATGAAAAGGTTATTTGGTAGAAAGTCACTGATCCGAGTCTCCAAGGCGGAGCACCAGAAGCTCCGACGGATGACAATGGCGCCGATCAGCGGGCATGCTGCGCTCGAGATGTACATCGATCATATTGAACACACAGTGATCAGTGGGTTGGAGGAATGGTCAAGCATGGAGAAGCCATTGAAGCTGTTGACAGAGATAAAAGAGCTTACCTTCAAACTTATTTGGAACATTTTTATGGGTTCAACTTCCATAGACTATACCACAAAAGAAATGGAGGCTTTGTATGATGATGTTGCACTTGGCTTATTCTGTTTCCCCATCAACTTTCCTGGCTTCAACTTCCATAAATCTCTCAAGGTGATAGATGAGACGCTTCGTCTTAGTAGTATTGCATTTGCTACTTTTCGTGAGGCAACAACTGATATTAATATCAATGGTAAACTTATACCAAAAGGATGGAAAGTTATACTTTGGCATCGAGGGCTGTACATGGATGAAGAGCTACATCCTTCCCCACAAGACTTCAATCCATCGAGATGGGATGATTTTATAGGCAACCCCGGTGCTTTTACTCCGTTTGGATTAGGAGTCAGGTTGTGCCCTGGACGTGATCTTGCCAAGCTCGAGATCTCAATTTTCCTTCACCATTTTCTCCTCAATTACAAGATCCAGCGATATAATCCACAATGCCAATTGACTTATCTACCACTTCCTCATCCCAAAGACAAATGCCTTGCAAGAGTGCTGAAAGCTGCAGGAGAATGTGTATAA

Coding sequence (CDS)

ATGAAAAGGTTATTTGGTAGAAAGTCACTGATCCGAGTCTCCAAGGCGGAGCACCAGAAGCTCCGACGGATGACAATGGCGCCGATCAGCGGGCATGCTGCGCTCGAGATGTACATCGATCATATTGAACACACAGTGATCAGTGGGTTGGAGGAATGGTCAAGCATGGAGAAGCCATTGAAGCTGTTGACAGAGATAAAAGAGCTTACCTTCAAACTTATTTGGAACATTTTTATGGGTTCAACTTCCATAGACTATACCACAAAAGAAATGGAGGCTTTGTATGATGATGTTGCACTTGGCTTATTCTGTTTCCCCATCAACTTTCCTGGCTTCAACTTCCATAAATCTCTCAAGGTGATAGATGAGACGCTTCGTCTTAGTAGTATTGCATTTGCTACTTTTCGTGAGGCAACAACTGATATTAATATCAATGGTAAACTTATACCAAAAGGATGGAAAGTTATACTTTGGCATCGAGGGCTGTACATGGATGAAGAGCTACATCCTTCCCCACAAGACTTCAATCCATCGAGATGGGATGATTTTATAGGCAACCCCGGTGCTTTTACTCCGTTTGGATTAGGAGTCAGGTTGTGCCCTGGACGTGATCTTGCCAAGCTCGAGATCTCAATTTTCCTTCACCATTTTCTCCTCAATTACAAGATCCAGCGATATAATCCACAATGCCAATTGACTTATCTACCACTTCCTCATCCCAAAGACAAATGCCTTGCAAGAGTGCTGAAAGCTGCAGGAGAATGTGTATAA

Protein sequence

MKRLFGRKSLIRVSKAEHQKLRRMTMAPISGHAALEMYIDHIEHTVISGLEEWSSMEKPLKLLTEIKELTFKLIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLKVIDETLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWDDFIGNPGAFTPFGLGVRLCPGRDLAKLEISIFLHHFLLNYKIQRYNPQCQLTYLPLPHPKDKCLARVLKAAGECV

Homology

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BLAST of Csor.00g010220.m01 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.0e-65
Identity = 138/372 (37.10%), Postives = 191/372 (51.34%), Query Frame = 0


Query: 1   MKRLFGRKSLIRVSKAEHQKLRRMTMAPISGHAALEMYIDHIEHTVISGLEEWSSMEKPL 60
           +K L   + +I VS AEH+  RR+  +PI GH AL MY++ +E  VI+ LEE SSM+ P+
Sbjct: 121 IKELARCRPMIDVSNAEHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSSMKHPV 180

Query: 61  KLLTEIKELTFKLIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSL-- 120
           +LL E+K+++FK I ++FMGS++ D   K++ + + D+  G+F  PIN PGF FHK+L  
Sbjct: 181 ELLKEMKKVSFKAIVHVFMGSSNQD-IIKKIGSSFTDLYNGMFSIPINVPGFTFHKALEA 240

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 241 RKKLAKIVQPVVDERRLMIENGPQEGSQRKDLIDILLEVKDENGRKLEDEDISDLLIGLL 300

Query: 181 ----------------------------------------------------------KV 240
                                                                     +V
Sbjct: 301 FAGHESTATSLMWSITYLTQHPHILKKAKEEQEEITRTRFSSQKQLSLKEIKQMVYLSQV 360

Query: 241 IDETLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRW 253
           IDETLR ++IAFATFREAT D+NING +IPKGW+V++W R ++MD E +P+P++FNPSRW
Sbjct: 361 IDETLRCANIAFATFREATADVNINGYIIPKGWRVLIWARAIHMDSEYYPNPEEFNPSRW 420

[Close]

BLAST of Csor.00g010220.m01 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 7.7e-58
Identity = 127/371 (34.23%), Postives = 179/371 (48.25%), Query Frame = 0


Query: 4   LFGRKSLIRVSKAEHQKLRRMTMAPISGHAALEMYIDHIEHTVISGLEEWSSMEK-PLKL 63
           L G KS + +S  +H+++R++T API+G  AL  Y+  I+ TV++ L  WSS E   ++ 
Sbjct: 136 LIGPKSFVNMSYDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVEF 195

Query: 64  LTEIKELTFKLIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLK--- 123
           LTE++ +TFK+I  IFM S + D T + +E  Y D+  G+    IN PGF ++++L+   
Sbjct: 196 LTELRRMTFKIIVQIFM-SGADDATMEALERSYTDLNYGMRAMAINLPGFAYYRALRARR 255

Query: 124 ------------------------------------------------------------ 183
                                                                       
Sbjct: 256 KLVSVLQGVLDGRRAAAAKGFKRSGAMDMMDRLIEAEDERGRRLADDEIVDVLIMYLNAG 315

Query: 184 --------------------------------------------------------VIDE 243
                                                                   V+DE
Sbjct: 316 HESSGHITMWATVFLQENPDIFARAKAEQEEIMRSIPATQNGLTLRDFKKMHFLSQVVDE 375

Query: 244 TLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWDDF 255
           TLR  +I+F +FR+AT DI +NG LIPKGWKV LW+R ++MD++++P P+ FNPSRW+  
Sbjct: 376 TLRCVNISFVSFRQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRWEGP 435

[Close]

BLAST of Csor.00g010220.m01 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 217.2 bits (552), Expect = 2.1e-55
Identity = 121/365 (33.15%), Postives = 175/365 (47.95%), Query Frame = 0


Query: 3   RLFGRKSLIRVSKAEHQKLRRMTMAPISGHAALEMYIDHIEHTVISGLEEWSSMEKPLKL 62
           +L GRKS + +S  EH++LRR+T AP++G  AL +YI  IE TV + LE+WS M + ++ 
Sbjct: 124 KLIGRKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEF 183

Query: 63  LTEIKELTFKLIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLK--- 122
           L+ +++LTFK+I  IF+ S S ++    +E  Y ++  G+    IN PGF +H++LK   
Sbjct: 184 LSHLRKLTFKVIMYIFLSSES-EHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARK 243

Query: 123 ------------------------------------------------------------ 182
                                                                       
Sbjct: 244 KLVAAFQSIVTNRRNQRKQNISSNRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGH 303

Query: 183 ------------------------------------------------------VIDETL 242
                                                                 VIDETL
Sbjct: 304 ESSGHLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQKLTLKETREMVYLSQVIDETL 363

Query: 243 RLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWDDFIG 251
           R+ + +   FREA +D+ ++G +IPKGWKV+ W R +++D E++P P+ F+PSRW+ +  
Sbjct: 364 RVITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTP 423

[Close]

BLAST of Csor.00g010220.m01 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 8.0e-55
Identity = 122/364 (33.52%), Postives = 172/364 (47.25%), Query Frame = 0


Query: 4   LFGRKSLIRVSKAEHQKLRRMTMAPISGHAALEMYIDHIEHTVISGLEEWSSMEKPLKLL 63
           L GRKS + +S  EH++LRR+T AP++GH AL  YI +IE  VI+ L++W+ M +  + L
Sbjct: 126 LIGRKSFVGISFEEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFL 185

Query: 64  TEIKELTFKLIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLK---- 123
           T +++LTF++I  IF+ S S +     +E  Y  +  G+    +N PGF +H++LK    
Sbjct: 186 THLRKLTFRIIMYIFLSSES-ENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKT 245

Query: 124 ------------------------------------------------------------ 183
                                                                       
Sbjct: 246 LVAAFQSIVTERRNQRKQNILSNKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHE 305

Query: 184 ------------------------------------------------------VIDETL 243
                                                                 V+DETL
Sbjct: 306 SSGHTIMWATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETL 365

Query: 244 RLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWDD-FI 249
           R+ + +   FREA TD+ +NG LIPKGWKV+ W R +++D E+ P P+ F+P+RWD+ F+
Sbjct: 366 RVITFSLTAFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFV 425

[Close]

BLAST of Csor.00g010220.m01 vs. ExPASy Swiss-Prot
Match: Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 1.4e-54
Identity = 120/369 (32.52%), Postives = 171/369 (46.34%), Query Frame = 0


Query: 4   LFGRKSLIRVSKAEHQKLRRMTMAPISGHAALEMYIDHIEHTVISGLEEWSSM--EKPLK 63
           L G KS + +   EH++LR++T API+G  AL  Y+  I+ TV++ L  WS    +   +
Sbjct: 126 LIGPKSFVNMGYDEHRRLRKLTAAPINGFDALTSYLGFIDDTVVTTLRGWSERGGDGHFE 185

Query: 64  LLTEIKELTFKLIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLK-- 123
            LTE++ +TF++I  IFMG    + T  E+E  Y ++  G+    I+ PGF +HK+++  
Sbjct: 186 FLTELRRMTFRIIVQIFMGGAD-ERTAAELERTYTELNYGMRAMAIDLPGFAYHKAIRAR 245

Query: 124 ------------------------------------------------------------ 183
                                                                       
Sbjct: 246 RRLVAALQRVLDERRARGGKTAAGAAAPVDMMDRLIAVEDEGGRRLQDDEIIDVLVMYLN 305

Query: 184 ----------------------------------------------------------VI 243
                                                                     V+
Sbjct: 306 AGHESSGHITMWATVFLQENPEILAKAKAEQEAIMRSIPPGQKGLTLRDFRKMAYLSQVV 365

Query: 244 DETLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWD 251
           DETLR  +I+F +FR+AT D+ +NG LIPKGWKV LW+R ++MD +++P P+ F+PSRW+
Sbjct: 366 DETLRFVNISFVSFRQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPKKFDPSRWE 425

[Close]

BLAST of Csor.00g010220.m01 vs. NCBI nr
Match: KAG6577616.1 (Beta-amyrin 11-oxidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 536 bits (1382), Expect = 1.02e-192
Identity = 256/256 (100.00%), Postives = 256/256 (100.00%), Query Frame = 0


Query: 1   MKRLFGRKSLIRVSKAEHQKLRRMTMAPISGHAALEMYIDHIEHTVISGLEEWSSMEKPL 60
           MKRLFGRKSLIRVSKAEHQKLRRMTMAPISGHAALEMYIDHIEHTVISGLEEWSSMEKPL
Sbjct: 1   MKRLFGRKSLIRVSKAEHQKLRRMTMAPISGHAALEMYIDHIEHTVISGLEEWSSMEKPL 60

Query: 61  KLLTEIKELTFKLIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLKV 120
           KLLTEIKELTFKLIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLKV
Sbjct: 61  KLLTEIKELTFKLIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLKV 120

Query: 121 IDETLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRW 180
           IDETLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRW
Sbjct: 121 IDETLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRW 180

Query: 181 DDFIGNPGAFTPFGLGVRLCPGRDLAKLEISIFLHHFLLNYKIQRYNPQCQLTYLPLPHP 240
           DDFIGNPGAFTPFGLGVRLCPGRDLAKLEISIFLHHFLLNYKIQRYNPQCQLTYLPLPHP
Sbjct: 181 DDFIGNPGAFTPFGLGVRLCPGRDLAKLEISIFLHHFLLNYKIQRYNPQCQLTYLPLPHP 240

Query: 241 KDKCLARVLKAAGECV 256
           KDKCLARVLKAAGECV
Sbjct: 241 KDKCLARVLKAAGECV 256

[Close]

BLAST of Csor.00g010220.m01 vs. NCBI nr
Match: XP_023552974.1 (beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 471 bits (1213), Expect = 1.35e-159
Identity = 246/374 (65.78%), Postives = 253/374 (67.65%), Query Frame = 0


Query: 1   MKRLFGRKSLIRVSKAEHQKLRRMTMAPISGHAALEMYIDHIEHTVISGLEEWSSMEKPL 60
           MKRLFGRKSLIRVSKAEH+KLRRMTMAPIS HAALE+YI+H EHTVISGLEEWSS+EKPL
Sbjct: 119 MKRLFGRKSLIRVSKAEHRKLRRMTMAPISSHAALEIYIEHTEHTVISGLEEWSSVEKPL 178

Query: 61  KLLTEIKELTFKLIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLK- 120
           KLLTEIKELTFK+IWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLK 
Sbjct: 179 KLLTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLKA 238

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 239 RKRLYEILLSIVNEKRLMKKSKGESWEAKDMMDLMTEVEDEDGEGMDNETITDLIFGKLF 298

Query: 181 ----------------------------------------------------------VI 240
                                                                     VI
Sbjct: 299 AGHETSAFTTMWAIIFLTDHPHIFQRAKEEQEDIIRRRPSTQKGINLVEFKQMKFLSQVI 358

Query: 241 DETLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWD 255
           DETLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWD
Sbjct: 359 DETLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWD 418

[Close]

BLAST of Csor.00g010220.m01 vs. NCBI nr
Match: KAG6577628.1 (Beta-amyrin 11-oxidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 433 bits (1113), Expect = 1.89e-148
Identity = 225/354 (63.56%), Postives = 240/354 (67.80%), Query Frame = 0


Query: 1   MKRLFGRKSLIRVSKAEHQKLRRMTMAPISGHAALEMYIDHIEHTVISGLEEWSSMEKPL 60
           MK LFGRKSL RVSKAEH+KLRR+T APISGHAALEMYI+HIEHTVISGLEEWSS+EKPL
Sbjct: 119 MKTLFGRKSLHRVSKAEHRKLRRVTTAPISGHAALEMYIEHIEHTVISGLEEWSSVEKPL 178

Query: 61  KLLTEIKELTFKLIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLKV 120
           KLLTEIKELTFK+IWNIFMGSTSIDYTTKEMEALY+D++LG F  PINFPGF FHKSLKV
Sbjct: 179 KLLTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKV 238

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 239 DEEKQREKLGSKDMMDLMIEVEDEDGEGMDNETIADLIFGKLFAGQETSAFTTMWAIIFL 298

Query: 181 ------------------------------------------IDETLRLSSIAFATFREA 240
                                                     IDETLRLS I FATFREA
Sbjct: 299 TDHPHIFQKAKEEQEDILSRRPSTQKGINLAEFKQMKFLSQVIDETLRLSGITFATFREA 358

Query: 241 TTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWDDFIGNPGAFTPFGLGVR 252
           TTD+NINGKLIPKGWKVILW R LYMDEELHPSPQDFNPSRWD+FIGNPGAFTPFGLGVR
Sbjct: 359 TTDVNINGKLIPKGWKVILWLRELYMDEELHPSPQDFNPSRWDNFIGNPGAFTPFGLGVR 418

[Close]

BLAST of Csor.00g010220.m01 vs. NCBI nr
Match: XP_023007801.1 (beta-amyrin 11-oxidase-like [Cucurbita maxima])

HSP 1 Score: 422 bits (1086), Expect = 4.13e-144
Identity = 223/371 (60.11%), Postives = 238/371 (64.15%), Query Frame = 0


Query: 1   MKRLFGRKSLIRVSKAEHQKLRRMTMAPISGHAALEMYIDHIEHTVISGLEEWSSMEKPL 60
           MK LFGRKSL RVSK EH+KLRR+T APISGHAALEMYI+HIEHTVISGLEEWSS+EKPL
Sbjct: 119 MKTLFGRKSLHRVSKVEHRKLRRVTTAPISGHAALEMYIEHIEHTVISGLEEWSSVEKPL 178

Query: 61  KLLTEIKELTFKLIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLK- 120
           KLLTEIKELTFK+IWNIFMGSTSIDYTTKEMEALY+D++LG F  PINFPGF FHKSLK 
Sbjct: 179 KLLTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKA 238

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 239 RKRLHEILLSIVNEKRLMKKSKGESWEAQDMMDLMIEVEDEDGEGMDNETIADLIFGKLF 298

Query: 181 ----------------------------------------------------------VI 240
                                                                     VI
Sbjct: 299 AGQETSAFTTMWAIIFLTDHPHLFQKAKEEQEDILSRRPSTQKSINFAEFKQMKFLSQVI 358

Query: 241 DETLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWD 252
           DETLRLS I FATFREATTD+NINGKLIPKGWKVILW R LYMDEELHPSPQDFNPSRWD
Sbjct: 359 DETLRLSGITFATFREATTDVNINGKLIPKGWKVILWLRELYMDEELHPSPQDFNPSRWD 418

[Close]

BLAST of Csor.00g010220.m01 vs. NCBI nr
Match: XP_022923281.1 (beta-amyrin 11-oxidase-like [Cucurbita moschata])

HSP 1 Score: 422 bits (1086), Expect = 4.13e-144
Identity = 223/371 (60.11%), Postives = 238/371 (64.15%), Query Frame = 0


Query: 1   MKRLFGRKSLIRVSKAEHQKLRRMTMAPISGHAALEMYIDHIEHTVISGLEEWSSMEKPL 60
           MK LFGRKSL RVSKAEH+KLRR+T APISGHAALEMYI+HIEHTVISGLEEWSS+EKPL
Sbjct: 119 MKTLFGRKSLHRVSKAEHRKLRRVTTAPISGHAALEMYIEHIEHTVISGLEEWSSVEKPL 178

Query: 61  KLLTEIKELTFKLIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLK- 120
           KLLTEIKELTFK+IWNIFMGSTSIDYTTKEMEALY+D++LG F  PINFPGF FHKSLK 
Sbjct: 179 KLLTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKA 238

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 239 RKRLHEILLSIVNEKRLMMKSKGESWEAKDMMDLMIEVEDEDGEGMDNETIADLIFGKLF 298

Query: 181 ----------------------------------------------------------VI 240
                                                                     VI
Sbjct: 299 AGQETSAFTTMWAIIFLTDHPHIFQKAKEEQEDILSRRPSTHKGINLAEFKQMKFLSQVI 358

Query: 241 DETLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWD 252
           DETLRLS I FATFREATTD+ INGKLIPKGWKVILW R LYMDEELHPSPQDFNPSRWD
Sbjct: 359 DETLRLSGITFATFREATTDVKINGKLIPKGWKVILWLRELYMDEELHPSPQDFNPSRWD 418

[Close]

BLAST of Csor.00g010220.m01 vs. ExPASy TrEMBL
Match: A0A6J1L1P3 (beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111500300 PE=3 SV=1)

HSP 1 Score: 422 bits (1086), Expect = 2.00e-144
Identity = 223/371 (60.11%), Postives = 238/371 (64.15%), Query Frame = 0


Query: 1   MKRLFGRKSLIRVSKAEHQKLRRMTMAPISGHAALEMYIDHIEHTVISGLEEWSSMEKPL 60
           MK LFGRKSL RVSK EH+KLRR+T APISGHAALEMYI+HIEHTVISGLEEWSS+EKPL
Sbjct: 119 MKTLFGRKSLHRVSKVEHRKLRRVTTAPISGHAALEMYIEHIEHTVISGLEEWSSVEKPL 178

Query: 61  KLLTEIKELTFKLIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLK- 120
           KLLTEIKELTFK+IWNIFMGSTSIDYTTKEMEALY+D++LG F  PINFPGF FHKSLK 
Sbjct: 179 KLLTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKA 238

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 239 RKRLHEILLSIVNEKRLMKKSKGESWEAQDMMDLMIEVEDEDGEGMDNETIADLIFGKLF 298

Query: 181 ----------------------------------------------------------VI 240
                                                                     VI
Sbjct: 299 AGQETSAFTTMWAIIFLTDHPHLFQKAKEEQEDILSRRPSTQKSINFAEFKQMKFLSQVI 358

Query: 241 DETLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWD 252
           DETLRLS I FATFREATTD+NINGKLIPKGWKVILW R LYMDEELHPSPQDFNPSRWD
Sbjct: 359 DETLRLSGITFATFREATTDVNINGKLIPKGWKVILWLRELYMDEELHPSPQDFNPSRWD 418

[Close]

BLAST of Csor.00g010220.m01 vs. ExPASy TrEMBL
Match: A0A6J1E6E2 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111431022 PE=3 SV=1)

HSP 1 Score: 422 bits (1086), Expect = 2.00e-144
Identity = 223/371 (60.11%), Postives = 238/371 (64.15%), Query Frame = 0


Query: 1   MKRLFGRKSLIRVSKAEHQKLRRMTMAPISGHAALEMYIDHIEHTVISGLEEWSSMEKPL 60
           MK LFGRKSL RVSKAEH+KLRR+T APISGHAALEMYI+HIEHTVISGLEEWSS+EKPL
Sbjct: 119 MKTLFGRKSLHRVSKAEHRKLRRVTTAPISGHAALEMYIEHIEHTVISGLEEWSSVEKPL 178

Query: 61  KLLTEIKELTFKLIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLK- 120
           KLLTEIKELTFK+IWNIFMGSTSIDYTTKEMEALY+D++LG F  PINFPGF FHKSLK 
Sbjct: 179 KLLTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKA 238

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 239 RKRLHEILLSIVNEKRLMMKSKGESWEAKDMMDLMIEVEDEDGEGMDNETIADLIFGKLF 298

Query: 181 ----------------------------------------------------------VI 240
                                                                     VI
Sbjct: 299 AGQETSAFTTMWAIIFLTDHPHIFQKAKEEQEDILSRRPSTHKGINLAEFKQMKFLSQVI 358

Query: 241 DETLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWD 252
           DETLRLS I FATFREATTD+ INGKLIPKGWKVILW R LYMDEELHPSPQDFNPSRWD
Sbjct: 359 DETLRLSGITFATFREATTDVKINGKLIPKGWKVILWLRELYMDEELHPSPQDFNPSRWD 418

[Close]

BLAST of Csor.00g010220.m01 vs. ExPASy TrEMBL
Match: A0A6J1L5Y8 (beta-amyrin 11-oxidase-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111500295 PE=3 SV=1)

HSP 1 Score: 418 bits (1074), Expect = 8.90e-144
Identity = 220/371 (59.30%), Postives = 236/371 (63.61%), Query Frame = 0


Query: 1   MKRLFGRKSLIRVSKAEHQKLRRMTMAPISGHAALEMYIDHIEHTVISGLEEWSSMEKPL 60
           M+ LFGR +L R SKAEH+KLRR+T APIS HAALEMY+DHIEHTVISGLEEWSS+EKPL
Sbjct: 38  MQTLFGRNTLHRASKAEHRKLRRVTTAPISSHAALEMYVDHIEHTVISGLEEWSSVEKPL 97

Query: 61  KLLTEIKELTFKLIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLK- 120
           KLLTEIKELTFK+IWNIFMGSTSIDYTTKEMEALY+D++LG F  PINFPGF FHKSLK 
Sbjct: 98  KLLTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKA 157

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 158 RKRLYEILLSIVNEKRLMKKSKGESWEAQDMMDLMIEVEDEDGEGMDNETIADLIFGKLF 217

Query: 181 ----------------------------------------------------------VI 240
                                                                     VI
Sbjct: 218 AGQETSAFTTMWAIIFLTDHPHLFQKAKEEQEDIISRRPSTQKGINLAEFKQMKFLSQVI 277

Query: 241 DETLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWD 252
           DETLRLS I FATFREATTD+NINGKLIPKGWKVILW R LYMDEELHPSPQDFNPSRWD
Sbjct: 278 DETLRLSGIIFATFREATTDVNINGKLIPKGWKVILWLRELYMDEELHPSPQDFNPSRWD 337

[Close]

BLAST of Csor.00g010220.m01 vs. ExPASy TrEMBL
Match: A0A6J1L8M7 (beta-amyrin 11-oxidase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111500295 PE=3 SV=1)

HSP 1 Score: 418 bits (1074), Expect = 1.32e-142
Identity = 220/371 (59.30%), Postives = 236/371 (63.61%), Query Frame = 0


Query: 1   MKRLFGRKSLIRVSKAEHQKLRRMTMAPISGHAALEMYIDHIEHTVISGLEEWSSMEKPL 60
           M+ LFGR +L R SKAEH+KLRR+T APIS HAALEMY+DHIEHTVISGLEEWSS+EKPL
Sbjct: 119 MQTLFGRNTLHRASKAEHRKLRRVTTAPISSHAALEMYVDHIEHTVISGLEEWSSVEKPL 178

Query: 61  KLLTEIKELTFKLIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLK- 120
           KLLTEIKELTFK+IWNIFMGSTSIDYTTKEMEALY+D++LG F  PINFPGF FHKSLK 
Sbjct: 179 KLLTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKA 238

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 239 RKRLYEILLSIVNEKRLMKKSKGESWEAQDMMDLMIEVEDEDGEGMDNETIADLIFGKLF 298

Query: 181 ----------------------------------------------------------VI 240
                                                                     VI
Sbjct: 299 AGQETSAFTTMWAIIFLTDHPHLFQKAKEEQEDIISRRPSTQKGINLAEFKQMKFLSQVI 358

Query: 241 DETLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWD 252
           DETLRLS I FATFREATTD+NINGKLIPKGWKVILW R LYMDEELHPSPQDFNPSRWD
Sbjct: 359 DETLRLSGIIFATFREATTDVNINGKLIPKGWKVILWLRELYMDEELHPSPQDFNPSRWD 418

[Close]

BLAST of Csor.00g010220.m01 vs. ExPASy TrEMBL
Match: A0A6J1KZN6 (beta-amyrin 11-oxidase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111500295 PE=3 SV=1)

HSP 1 Score: 418 bits (1074), Expect = 1.55e-142
Identity = 220/371 (59.30%), Postives = 236/371 (63.61%), Query Frame = 0


Query: 1   MKRLFGRKSLIRVSKAEHQKLRRMTMAPISGHAALEMYIDHIEHTVISGLEEWSSMEKPL 60
           M+ LFGR +L R SKAEH+KLRR+T APIS HAALEMY+DHIEHTVISGLEEWSS+EKPL
Sbjct: 124 MQTLFGRNTLHRASKAEHRKLRRVTTAPISSHAALEMYVDHIEHTVISGLEEWSSVEKPL 183

Query: 61  KLLTEIKELTFKLIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLK- 120
           KLLTEIKELTFK+IWNIFMGSTSIDYTTKEMEALY+D++LG F  PINFPGF FHKSLK 
Sbjct: 184 KLLTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKA 243

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 244 RKRLYEILLSIVNEKRLMKKSKGESWEAQDMMDLMIEVEDEDGEGMDNETIADLIFGKLF 303

Query: 181 ----------------------------------------------------------VI 240
                                                                     VI
Sbjct: 304 AGQETSAFTTMWAIIFLTDHPHLFQKAKEEQEDIISRRPSTQKGINLAEFKQMKFLSQVI 363

Query: 241 DETLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWD 252
           DETLRLS I FATFREATTD+NINGKLIPKGWKVILW R LYMDEELHPSPQDFNPSRWD
Sbjct: 364 DETLRLSGIIFATFREATTDVNINGKLIPKGWKVILWLRELYMDEELHPSPQDFNPSRWD 423

[Close]

BLAST of Csor.00g010220.m01 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 217.2 bits (552), Expect = 1.5e-56
Identity = 121/365 (33.15%), Postives = 175/365 (47.95%), Query Frame = 0


Query: 3   RLFGRKSLIRVSKAEHQKLRRMTMAPISGHAALEMYIDHIEHTVISGLEEWSSMEKPLKL 62
           +L GRKS + +S  EH++LRR+T AP++G  AL +YI  IE TV + LE+WS M + ++ 
Sbjct: 124 KLIGRKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEF 183

Query: 63  LTEIKELTFKLIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLK--- 122
           L+ +++LTFK+I  IF+ S S ++    +E  Y ++  G+    IN PGF +H++LK   
Sbjct: 184 LSHLRKLTFKVIMYIFLSSES-EHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARK 243

Query: 123 ------------------------------------------------------------ 182
                                                                       
Sbjct: 244 KLVAAFQSIVTNRRNQRKQNISSNRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGH 303

Query: 183 ------------------------------------------------------VIDETL 242
                                                                 VIDETL
Sbjct: 304 ESSGHLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQKLTLKETREMVYLSQVIDETL 363

Query: 243 RLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWDDFIG 251
           R+ + +   FREA +D+ ++G +IPKGWKV+ W R +++D E++P P+ F+PSRW+ +  
Sbjct: 364 RVITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTP 423

[Close]

BLAST of Csor.00g010220.m01 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 217.2 bits (552), Expect = 1.5e-56
Identity = 121/365 (33.15%), Postives = 175/365 (47.95%), Query Frame = 0


Query: 3   RLFGRKSLIRVSKAEHQKLRRMTMAPISGHAALEMYIDHIEHTVISGLEEWSSMEKPLKL 62
           +L GRKS + +S  EH++LRR+T AP++G  AL +YI  IE TV + LE+WS M + ++ 
Sbjct: 124 KLIGRKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEF 183

Query: 63  LTEIKELTFKLIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLK--- 122
           L+ +++LTFK+I  IF+ S S ++    +E  Y ++  G+    IN PGF +H++LK   
Sbjct: 184 LSHLRKLTFKVIMYIFLSSES-EHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARK 243

Query: 123 ------------------------------------------------------------ 182
                                                                       
Sbjct: 244 KLVAAFQSIVTNRRNQRKQNISSNRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGH 303

Query: 183 ------------------------------------------------------VIDETL 242
                                                                 VIDETL
Sbjct: 304 ESSGHLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQKLTLKETREMVYLSQVIDETL 363

Query: 243 RLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWDDFIG 251
           R+ + +   FREA +D+ ++G +IPKGWKV+ W R +++D E++P P+ F+PSRW+ +  
Sbjct: 364 RVITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTP 423

[Close]

BLAST of Csor.00g010220.m01 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 215.3 bits (547), Expect = 5.7e-56
Identity = 122/364 (33.52%), Postives = 172/364 (47.25%), Query Frame = 0


Query: 4   LFGRKSLIRVSKAEHQKLRRMTMAPISGHAALEMYIDHIEHTVISGLEEWSSMEKPLKLL 63
           L GRKS + +S  EH++LRR+T AP++GH AL  YI +IE  VI+ L++W+ M +  + L
Sbjct: 126 LIGRKSFVGISFEEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFL 185

Query: 64  TEIKELTFKLIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLK---- 123
           T +++LTF++I  IF+ S S +     +E  Y  +  G+    +N PGF +H++LK    
Sbjct: 186 THLRKLTFRIIMYIFLSSES-ENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKT 245

Query: 124 ------------------------------------------------------------ 183
                                                                       
Sbjct: 246 LVAAFQSIVTERRNQRKQNILSNKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHE 305

Query: 184 ------------------------------------------------------VIDETL 243
                                                                 V+DETL
Sbjct: 306 SSGHTIMWATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETL 365

Query: 244 RLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWDD-FI 249
           R+ + +   FREA TD+ +NG LIPKGWKV+ W R +++D E+ P P+ F+P+RWD+ F+
Sbjct: 366 RVITFSLTAFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFV 425

[Close]

BLAST of Csor.00g010220.m01 vs. TAIR 10
Match: AT3G19270.1 (cytochrome P450, family 707, subfamily A, polypeptide 4 )

HSP 1 Score: 112.5 bits (280), Expect = 5.2e-25
Identity = 54/124 (43.55%), Postives = 78/124 (62.90%), Query Frame = 0


Query: 119 KVIDETLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPS 178
           KVI E+LR++SI   TFREA  D+   G LIPKGWKV+   R ++ + +   +P+ F+PS
Sbjct: 335 KVIVESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNPKYFSNPEVFDPS 394

Query: 179 RWDDFIGNPGAFTPFGLGVRLCPGRDLAKLEISIFLHHFLLNYKIQRYNPQCQLTYLPLP 238
           R+ +    P  F PFG GV  CPG +LAKL+I IFLHH + N++ +    +  + Y P P
Sbjct: 395 RF-EVNPKPNTFMPFGSGVHACPGNELAKLQILIFLHHLVSNFRWEVKGGEKGIQYSPFP 454

Query: 239 HPKD 243
            P++
Sbjct: 455 IPQN 457

[Close]

BLAST of Csor.00g010220.m01 vs. TAIR 10
Match: AT4G36380.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 112.1 bits (279), Expect = 6.8e-25
Identity = 52/105 (49.52%), Postives = 72/105 (68.57%), Query Frame = 0


Query: 120 VIDETLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSR 179
           VI+ETLR+++I    +R+A  D+ I G LIPKGW V+     ++MDE+++ +P  F+P R
Sbjct: 380 VINETLRMANIINGVWRKALKDVEIKGYLIPKGWCVLASFISVHMDEDIYDNPYQFDPWR 439

Query: 180 WDDFIGNPGA---FTPFGLGVRLCPGRDLAKLEISIFLHHFLLNY 222
           WD   G+  +   FTPFG G RLCPG +L+KLEISIFLHH +  Y
Sbjct: 440 WDRINGSANSSICFTPFGGGQRLCPGLELSKLEISIFLHHLVTRY 484

The following BLAST results are available for this feature:

BLAST of Csor.00g010220.m01 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B5BSX1	1.0e-65	37.10	Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1	[more]
Q5VRM7	7.7e-58	34.23	Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ...	[more]
Q9C5Y2	2.1e-55	33.15	Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2	[more]
O23051	8.0e-55	33.52	Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1	[more]
Q9AXH9	1.4e-54	32.52	Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1	[more]

BLAST of Csor.00g010220.m01 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6577616.1	1.02e-192	100.00	Beta-amyrin 11-oxidase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023552974.1	1.35e-159	65.78	beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo]	[more]
KAG6577628.1	1.89e-148	63.56	Beta-amyrin 11-oxidase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023007801.1	4.13e-144	60.11	beta-amyrin 11-oxidase-like [Cucurbita maxima]	[more]
XP_022923281.1	4.13e-144	60.11	beta-amyrin 11-oxidase-like [Cucurbita moschata]	[more]

BLAST of Csor.00g010220.m01 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1L1P3	2.00e-144	60.11	beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111500300 PE=3 SV=...	[more]
A0A6J1E6E2	2.00e-144	60.11	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111431022 PE=3 S...	[more]
A0A6J1L5Y8	8.90e-144	59.30	beta-amyrin 11-oxidase-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC1115002...	[more]
A0A6J1L8M7	1.32e-142	59.30	beta-amyrin 11-oxidase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1115002...	[more]
A0A6J1KZN6	1.55e-142	59.30	beta-amyrin 11-oxidase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1115002...	[more]

BLAST of Csor.00g010220.m01 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G32440.1	1.5e-56	33.15	ent-kaurenoic acid hydroxylase 2	[more]
AT2G32440.2	1.5e-56	33.15	ent-kaurenoic acid hydroxylase 2	[more]
AT1G05160.1	5.7e-56	33.52	cytochrome P450, family 88, subfamily A, polypeptide 3	[more]
AT3G19270.1	5.2e-25	43.55	cytochrome P450, family 707, subfamily A, polypeptide 4	[more]
AT4G36380.1	6.8e-25	49.52	Cytochrome P450 superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 119..130 score: 41.37 coord: 191..200 score: 54.72 coord: 200..211 score: 40.16
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 119..225 e-value: 9.9E-32 score: 110.3
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 158..182 score: 25.12 coord: 190..200 score: 53.04 coord: 200..223 score: 30.6
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 118..250 e-value: 6.6E-36 score: 125.9
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..117 e-value: 1.7E-14 score: 55.5
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 3..248
None	No IPR available	PANTHER	PTHR24286:SF199	CYTOCHROME P450 88D6	coord: 120..229 coord: 2..119
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 120..229 coord: 2..119
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 193..202

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Csor.00g010220	Csor.00g010220	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csor.00g010220.m01.CDS01	Csor.00g010220.m01.CDS01	CDS
Csor.00g010220.m01.CDS02	Csor.00g010220.m01.CDS02	CDS
Csor.00g010220.m01.CDS03	Csor.00g010220.m01.CDS03	CDS
Csor.00g010220.m01.CDS04	Csor.00g010220.m01.CDS04	CDS
Csor.00g010220.m01.CDS05	Csor.00g010220.m01.CDS05	CDS

The following stop_codon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csor.00g010220.m01.stop_codon01	Csor.00g010220.m01.stop_codon01	stop_codon

The following terminal feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csor.00g010220.m01.terminal01	Csor.00g010220.m01.terminal01	terminal

The following intron feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csor.00g010220.m01.intron01	Csor.00g010220.m01.intron01	intron
Csor.00g010220.m01.intron02	Csor.00g010220.m01.intron02	intron
Csor.00g010220.m01.intron03	Csor.00g010220.m01.intron03	intron
Csor.00g010220.m01.intron04	Csor.00g010220.m01.intron04	intron

The following internal feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csor.00g010220.m01.internal01	Csor.00g010220.m01.internal01	internal
Csor.00g010220.m01.internal02	Csor.00g010220.m01.internal02	internal
Csor.00g010220.m01.internal03	Csor.00g010220.m01.internal03	internal

The following initial feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csor.00g010220.m01.initial01	Csor.00g010220.m01.initial01	initial

The following start_codon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csor.00g010220.m01.start_codon01	Csor.00g010220.m01.start_codon01	start_codon

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Csor.00g010220.m01	Csor.00g010220.m01-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen