CsaV3_6G024180.1 (mRNA) Cucumber (Chinese Long) v3

Overview
NameCsaV3_6G024180.1
TypemRNA
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
DescriptionNADH dehydrogenase subunit 7
Locationchr6: 15998121 .. 15998579 (+)
Sequence length219
RNA-Seq ExpressionCsaV3_6G024180.1
SyntenyCsaV3_6G024180.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCAATCGGCAACCGTATCTGGAAACAACGATTAGTGGATATTGGTACTGTCACTGCACAGCAAGCAAAGGATTGGGGATTCAGTGGTGTAATGTTAAGAGGTCGTGCGACATGAAGACATTGATAGCAATATGGGGGAAGTTCCCATCAGGCAACAAGGGTTCTGCCTGACCCTACTTAAGCATGCATATTATGTCAGTGAAGACTTGGTGTGAAGCCTTGGAGCTTACGTTAGAAGAGCAAAAGGCCCGGGGCTAGGGTAAGCTGAGGGGGGACAGCGTAAGTGAGCGAATGTGTGTAAGCCCAGTCAAAGATGACTGTTCTAGGCGGGGCGGGCCACCCACCTGAGAATGGTCTTGGTCCTACGGACCGTGAACGGATTCGCCTCTGGCCTCTGGGTACGTCTCCACCGCATAAGTTCACCGGGGTGGAGCACGGTCCGCCAAAATCGGCATAG

mRNA sequence

ATGTCAATCGGCAACCGTATCTGGAAACAACGATTAGTGGATATTGGTACTGTCACTGCACAGCAAGCAAAGGATTGGGGATTCAGTGGCGGGGCGGGCCACCCACCTGAGAATGGTCTTGGTCCTACGGACCGTGAACGGATTCGCCTCTGGCCTCTGGGTACGTCTCCACCGCATAAGTTCACCGGGGTGGAGCACGGTCCGCCAAAATCGGCATAG

Coding sequence (CDS)

ATGTCAATCGGCAACCGTATCTGGAAACAACGATTAGTGGATATTGGTACTGTCACTGCACAGCAAGCAAAGGATTGGGGATTCAGTGGCGGGGCGGGCCACCCACCTGAGAATGGTCTTGGTCCTACGGACCGTGAACGGATTCGCCTCTGGCCTCTGGGTACGTCTCCACCGCATAAGTTCACCGGGGTGGAGCACGGTCCGCCAAAATCGGCATAG

Protein sequence

MSIGNRIWKQRLVDIGTVTAQQAKDWGFSGGAGHPPENGLGPTDRERIRLWPLGTSPPHKFTGVEHGPPKSA*
Homology
BLAST of CsaV3_6G024180.1 vs. NCBI nr
Match: KAE8647122.1 (hypothetical protein Csa_021731 [Cucumis sativus])

HSP 1 Score: 162.2 bits (409), Expect = 1.7e-36
Identity = 72/72 (100.00%), Postives = 72/72 (100.00%), Query Frame = 0

Query: 1  MSIGNRIWKQRLVDIGTVTAQQAKDWGFSGGAGHPPENGLGPTDRERIRLWPLGTSPPHK 60
          MSIGNRIWKQRLVDIGTVTAQQAKDWGFSGGAGHPPENGLGPTDRERIRLWPLGTSPPHK
Sbjct: 1  MSIGNRIWKQRLVDIGTVTAQQAKDWGFSGGAGHPPENGLGPTDRERIRLWPLGTSPPHK 60

Query: 61 FTGVEHGPPKSA 73
          FTGVEHGPPKSA
Sbjct: 61 FTGVEHGPPKSA 72

BLAST of CsaV3_6G024180.1 vs. NCBI nr
Match: TYK06543.1 (NADH dehydrogenase subunit 7 [Cucumis melo var. makuwa])

HSP 1 Score: 154.1 bits (388), Expect = 4.7e-34
Identity = 71/77 (92.21%), Postives = 71/77 (92.21%), Query Frame = 0

Query: 1   MSIGNRIWKQRLVDIGTVTAQQAKDWGFS-----GGAGHPPENGLGPTDRERIRLWPLGT 60
           MS GNRIWKQRLVDIGTVTAQQAKDWGFS     GGAGHPPENGLGPTDRERIRLWPLGT
Sbjct: 136 MSTGNRIWKQRLVDIGTVTAQQAKDWGFSGVMLRGGAGHPPENGLGPTDRERIRLWPLGT 195

Query: 61  SPPHKFTGVEHGPPKSA 73
           SPPHKFTGVEHGPPKSA
Sbjct: 196 SPPHKFTGVEHGPPKSA 212

BLAST of CsaV3_6G024180.1 vs. NCBI nr
Match: KAF1856959.1 (hypothetical protein Lal_00002471 [Lupinus albus])

HSP 1 Score: 143.7 bits (361), Expect = 6.4e-31
Identity = 66/78 (84.62%), Postives = 68/78 (87.18%), Query Frame = 0

Query: 1   MSIGNRIWKQRLVDIGTVTAQQAKDWGFSG------GAGHPPENGLGPTDRERIRLWPLG 60
           MS GNRIWKQRLVDIGTVTAQQAKDWGFSG      GAGHPP NG+GPTDRERIRLWPLG
Sbjct: 117 MSTGNRIWKQRLVDIGTVTAQQAKDWGFSGVMLRGRGAGHPPSNGVGPTDRERIRLWPLG 176

Query: 61  TSPPHKFTGVEHGPPKSA 73
           TS PH+FTGVEH PPKSA
Sbjct: 177 TSEPHEFTGVEHSPPKSA 194

BLAST of CsaV3_6G024180.1 vs. NCBI nr
Match: KAF1890196.1 (hypothetical protein Lal_00025529 [Lupinus albus])

HSP 1 Score: 138.7 bits (348), Expect = 2.1e-29
Identity = 64/78 (82.05%), Postives = 66/78 (84.62%), Query Frame = 0

Query: 1   MSIGNRIWKQRLVDIGTVTAQQAKDWGFSG------GAGHPPENGLGPTDRERIRLWPLG 60
           MS GNRIWKQRLVDIGTVTAQQAKDWGFSG      GAGHPP NG+GPTD ERIRLWPLG
Sbjct: 117 MSTGNRIWKQRLVDIGTVTAQQAKDWGFSGVMLRGRGAGHPPSNGVGPTDHERIRLWPLG 176

Query: 61  TSPPHKFTGVEHGPPKSA 73
           TS PH+FT VEH PPKSA
Sbjct: 177 TSEPHEFTRVEHSPPKSA 194

BLAST of CsaV3_6G024180.1 vs. NCBI nr
Match: PNX98164.1 (NADH dehydrogenase subunit 7 [Trifolium pratense])

HSP 1 Score: 137.9 bits (346), Expect = 3.5e-29
Identity = 63/72 (87.50%), Postives = 65/72 (90.28%), Query Frame = 0

Query: 1   MSIGNRIWKQRLVDIGTVTAQQAKDWGFSGGAGHPPENGLGPTDRERIRLWPLGTSPPHK 60
           MS GNRIWKQRLVDIGTVTAQQAKDWGFS GAGHPPE G+GPTDRE IRLWP GTS PH+
Sbjct: 70  MSTGNRIWKQRLVDIGTVTAQQAKDWGFS-GAGHPPEEGVGPTDREWIRLWPRGTSEPHE 129

Query: 61  FTGVEHGPPKSA 73
           FTGVEH PPKSA
Sbjct: 130 FTGVEHSPPKSA 140

BLAST of CsaV3_6G024180.1 vs. ExPASy Swiss-Prot
Match: Q36450 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 OS=Nicotiana sylvestris OX=4096 GN=NAD7 PE=2 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 2.9e-10
Identity = 29/30 (96.67%), Postives = 29/30 (96.67%), Query Frame = 0

Query: 1   MSIGNRIWKQRLVDIGTVTAQQAKDWGFSG 31
           MS GNRIWKQRLVDIGTVTAQQAKDWGFSG
Sbjct: 192 MSTGNRIWKQRLVDIGTVTAQQAKDWGFSG 221

BLAST of CsaV3_6G024180.1 vs. ExPASy Swiss-Prot
Match: P93306 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 OS=Arabidopsis thaliana OX=3702 GN=NAD7 PE=1 SV=2)

HSP 1 Score: 63.2 bits (152), Expect = 1.4e-09
Identity = 28/30 (93.33%), Postives = 28/30 (93.33%), Query Frame = 0

Query: 1   MSIGNRIWKQRLVDIGTVTAQQAKDWGFSG 31
           M  GNRIWKQRLVDIGTVTAQQAKDWGFSG
Sbjct: 192 MLTGNRIWKQRLVDIGTVTAQQAKDWGFSG 221

BLAST of CsaV3_6G024180.1 vs. ExPASy Swiss-Prot
Match: O21270 (NADH-ubiquinone oxidoreductase 49 kDa subunit OS=Reclinomonas americana OX=48483 GN=NAD7 PE=3 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 1.1e-06
Identity = 22/26 (84.62%), Postives = 25/26 (96.15%), Query Frame = 0

Query: 5   NRIWKQRLVDIGTVTAQQAKDWGFSG 31
           NRIWKQRLVDIGTV+A++A DWGFSG
Sbjct: 198 NRIWKQRLVDIGTVSAEEALDWGFSG 223

BLAST of CsaV3_6G024180.1 vs. ExPASy Swiss-Prot
Match: Q37619 (NADH-ubiquinone oxidoreductase 49 kDa subunit OS=Prototheca wickerhamii OX=3111 GN=NAD7 PE=3 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 7.4e-06
Identity = 21/30 (70.00%), Postives = 24/30 (80.00%), Query Frame = 0

Query: 1   MSIGNRIWKQRLVDIGTVTAQQAKDWGFSG 31
           M   NRIWKQRLVD+G V+A+QA DW FSG
Sbjct: 198 MLTSNRIWKQRLVDVGVVSAEQALDWSFSG 227

BLAST of CsaV3_6G024180.1 vs. ExPASy Swiss-Prot
Match: Q37720 (NADH-ubiquinone oxidoreductase 49 kDa subunit OS=Pylaiella littoralis OX=2885 GN=NAD7 PE=3 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 1.3e-05
Identity = 21/30 (70.00%), Postives = 25/30 (83.33%), Query Frame = 0

Query: 1   MSIGNRIWKQRLVDIGTVTAQQAKDWGFSG 31
           M   NRIW++RLVDIG V+AQ+A DWGFSG
Sbjct: 196 MLTDNRIWQERLVDIGVVSAQEAIDWGFSG 225

BLAST of CsaV3_6G024180.1 vs. ExPASy TrEMBL
Match: A0A5D3C3P7 (NADH dehydrogenase subunit 7 (Mitochondrion) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84067G00020 PE=3 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 2.3e-34
Identity = 71/77 (92.21%), Postives = 71/77 (92.21%), Query Frame = 0

Query: 1   MSIGNRIWKQRLVDIGTVTAQQAKDWGFS-----GGAGHPPENGLGPTDRERIRLWPLGT 60
           MS GNRIWKQRLVDIGTVTAQQAKDWGFS     GGAGHPPENGLGPTDRERIRLWPLGT
Sbjct: 136 MSTGNRIWKQRLVDIGTVTAQQAKDWGFSGVMLRGGAGHPPENGLGPTDRERIRLWPLGT 195

Query: 61  SPPHKFTGVEHGPPKSA 73
           SPPHKFTGVEHGPPKSA
Sbjct: 196 SPPHKFTGVEHGPPKSA 212

BLAST of CsaV3_6G024180.1 vs. ExPASy TrEMBL
Match: A0A6A5L872 (Complex1_49kDa domain-containing protein OS=Lupinus albus OX=3870 GN=Lal_00002471 PE=3 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 3.1e-31
Identity = 66/78 (84.62%), Postives = 68/78 (87.18%), Query Frame = 0

Query: 1   MSIGNRIWKQRLVDIGTVTAQQAKDWGFSG------GAGHPPENGLGPTDRERIRLWPLG 60
           MS GNRIWKQRLVDIGTVTAQQAKDWGFSG      GAGHPP NG+GPTDRERIRLWPLG
Sbjct: 117 MSTGNRIWKQRLVDIGTVTAQQAKDWGFSGVMLRGRGAGHPPSNGVGPTDRERIRLWPLG 176

Query: 61  TSPPHKFTGVEHGPPKSA 73
           TS PH+FTGVEH PPKSA
Sbjct: 177 TSEPHEFTGVEHSPPKSA 194

BLAST of CsaV3_6G024180.1 vs. ExPASy TrEMBL
Match: A0A6A5NZ26 (Complex1_49kDa domain-containing protein OS=Lupinus albus OX=3870 GN=Lal_00025529 PE=3 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.0e-29
Identity = 64/78 (82.05%), Postives = 66/78 (84.62%), Query Frame = 0

Query: 1   MSIGNRIWKQRLVDIGTVTAQQAKDWGFSG------GAGHPPENGLGPTDRERIRLWPLG 60
           MS GNRIWKQRLVDIGTVTAQQAKDWGFSG      GAGHPP NG+GPTD ERIRLWPLG
Sbjct: 117 MSTGNRIWKQRLVDIGTVTAQQAKDWGFSGVMLRGRGAGHPPSNGVGPTDHERIRLWPLG 176

Query: 61  TSPPHKFTGVEHGPPKSA 73
           TS PH+FT VEH PPKSA
Sbjct: 177 TSEPHEFTRVEHSPPKSA 194

BLAST of CsaV3_6G024180.1 vs. ExPASy TrEMBL
Match: A0A2K3N589 (NADH dehydrogenase subunit 7 OS=Trifolium pratense OX=57577 GN=L195_g021406 PE=3 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.7e-29
Identity = 63/72 (87.50%), Postives = 65/72 (90.28%), Query Frame = 0

Query: 1   MSIGNRIWKQRLVDIGTVTAQQAKDWGFSGGAGHPPENGLGPTDRERIRLWPLGTSPPHK 60
           MS GNRIWKQRLVDIGTVTAQQAKDWGFS GAGHPPE G+GPTDRE IRLWP GTS PH+
Sbjct: 70  MSTGNRIWKQRLVDIGTVTAQQAKDWGFS-GAGHPPEEGVGPTDREWIRLWPRGTSEPHE 129

Query: 61  FTGVEHGPPKSA 73
           FTGVEH PPKSA
Sbjct: 130 FTGVEHSPPKSA 140

BLAST of CsaV3_6G024180.1 vs. ExPASy TrEMBL
Match: A0A2Z6LN36 (Complex1_49kDa domain-containing protein OS=Trifolium subterraneum OX=3900 GN=TSUD_198960 PE=3 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 2.2e-24
Identity = 55/63 (87.30%), Postives = 57/63 (90.48%), Query Frame = 0

Query: 1   MSIGNRIWKQRLVDIGTVTAQQAKDWGFSGGAGHPPENGLGPTDRERIRLWPLGTSPPHK 60
           MS GNRIWKQRLVDIGTVTAQQAKDWGFS GAGHPP NG+GPTDRE IRLWP GTS PH+
Sbjct: 117 MSTGNRIWKQRLVDIGTVTAQQAKDWGFS-GAGHPPSNGVGPTDREWIRLWPRGTSEPHE 176

Query: 61  FTG 64
           FTG
Sbjct: 177 FTG 178

BLAST of CsaV3_6G024180.1 vs. TAIR 10
Match: ATMG00510.1 (NADH dehydrogenase subunit 7 )

HSP 1 Score: 65.5 bits (158), Expect = 2.1e-11
Identity = 29/30 (96.67%), Postives = 29/30 (96.67%), Query Frame = 0

Query: 1   MSIGNRIWKQRLVDIGTVTAQQAKDWGFSG 31
           MS GNRIWKQRLVDIGTVTAQQAKDWGFSG
Sbjct: 192 MSTGNRIWKQRLVDIGTVTAQQAKDWGFSG 221

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAE8647122.11.7e-36100.00hypothetical protein Csa_021731 [Cucumis sativus][more]
TYK06543.14.7e-3492.21NADH dehydrogenase subunit 7 [Cucumis melo var. makuwa][more]
KAF1856959.16.4e-3184.62hypothetical protein Lal_00002471 [Lupinus albus][more]
KAF1890196.12.1e-2982.05hypothetical protein Lal_00025529 [Lupinus albus][more]
PNX98164.13.5e-2987.50NADH dehydrogenase subunit 7 [Trifolium pratense][more]
Match NameE-valueIdentityDescription
Q364502.9e-1096.67NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 OS=Nicotiana sylvestris OX... [more]
P933061.4e-0993.33NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 OS=Arabidopsis thaliana OX... [more]
O212701.1e-0684.62NADH-ubiquinone oxidoreductase 49 kDa subunit OS=Reclinomonas americana OX=48483... [more]
Q376197.4e-0670.00NADH-ubiquinone oxidoreductase 49 kDa subunit OS=Prototheca wickerhamii OX=3111 ... [more]
Q377201.3e-0570.00NADH-ubiquinone oxidoreductase 49 kDa subunit OS=Pylaiella littoralis OX=2885 GN... [more]
Match NameE-valueIdentityDescription
A0A5D3C3P72.3e-3492.21NADH dehydrogenase subunit 7 (Mitochondrion) OS=Cucumis melo var. makuwa OX=1194... [more]
A0A6A5L8723.1e-3184.62Complex1_49kDa domain-containing protein OS=Lupinus albus OX=3870 GN=Lal_0000247... [more]
A0A6A5NZ261.0e-2982.05Complex1_49kDa domain-containing protein OS=Lupinus albus OX=3870 GN=Lal_0002552... [more]
A0A2K3N5891.7e-2987.50NADH dehydrogenase subunit 7 OS=Trifolium pratense OX=57577 GN=L195_g021406 PE=3... [more]
A0A2Z6LN362.2e-2487.30Complex1_49kDa domain-containing protein OS=Trifolium subterraneum OX=3900 GN=TS... [more]
Match NameE-valueIdentityDescription
ATMG00510.12.1e-1196.67NADH dehydrogenase subunit 7 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029014[NiFe]-hydrogenase, large subunitGENE3D1.10.645.10coord: 1..34
e-value: 3.6E-7
score: 31.3
IPR029014[NiFe]-hydrogenase, large subunitSUPERFAMILY56762HydB/Nqo4-likecoord: 3..33
IPR001135NADH-quinone oxidoreductase, subunit DPFAMPF00346Complex1_49kDacoord: 3..30
e-value: 8.6E-6
score: 25.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 26..72

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CsaV3_6G024180CsaV3_6G024180gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CsaV3_6G024180.1.exon1CsaV3_6G024180.1.exon1exon
CsaV3_6G024180.1.exon2CsaV3_6G024180.1.exon2exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CsaV3_6G024180.1.cds1CsaV3_6G024180.1.cds1CDS
CsaV3_6G024180.1.cds2CsaV3_6G024180.1.cds2CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CsaV3_6G024180.1CsaV3_6G024180.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051287 NAD binding
molecular_function GO:0016651 oxidoreductase activity, acting on NAD(P)H
molecular_function GO:0048038 quinone binding