CsaV3_5G032220.1 (mRNA) Cucumber (Chinese Long) v3

Overview
NameCsaV3_5G032220.1
TypemRNA
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
DescriptionProtein NRT1/ PTR FAMILY 2.13-like
Locationchr5: 26139363 .. 26143062 (-)
Sequence length1797
RNA-Seq ExpressionCsaV3_5G032220.1
SyntenyCsaV3_5G032220.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGTAGCTCATGACATCAACATACATACGGTGTATGTTGAGGATTAGAATATTAATTTGTTTTTGTTTGGTATGTATGTGATGAGAAAGATGCATATTGCATTTTGAGTTGTGTTTTAAAGTGATTACTTTTTCTATAAATACAAACACACCCAATAATGACAAAGAGGCAGAAACGAGAGAGAGAGGGGTAGAGATAATGGAGAGTGAGAGTGAAAGAAAAGCTTCGAGCAATTCTCGTAGTCCTAACAACTCCAAAGATGCTAATAATAATCCCAAGCTTTCAATAATATCCAACAAATCTAAAAGAGAGCCTGGAGGATGGAAGAGCATGCCTTACATTCTTGGTAATTTAATTTCTGATATTCTTCTTCTCTTTCTAAATTCTTGTTTGTTTTTGAGAGGTTATGTTATGTATATCTAATACTATTATTGTTGGGGATGCAGGAAATGAAACATTTGAAAGACTAGCTGCAATGGGATTACTAGCAAATTTCATGGTGTATCTAAAAAAAATGTATCATATGGATCAAGTTTCGGCGACCAGCTTAATGGGGTTATGGACGGGAGTCACCAGTTTCTTGCCTCTTCTCGGCGCATTTCTCTCCGACGCTTATATTGGCCGCTACTGGACTATCGCTATTGCTTCCATTTTTTCCTTTCTGGTACCTGAACTAAATCACTCCCCCCCTTTTTTAATGGCTCTTCTTCTTCGTATACTAACACAGCAAATTCTAGAAACGGGTTCACGTGGATTTTTAGCCGTTAAATTAAACAGATTATTGAGATTTCTCGTGTTCTTGCGTCCTTTTTCTCTCTCTACTCGTTCTCGTTCTGCAACACAAATTAAAAAGAGGGAGAGTGAAGAAGAGAACTGAAGAACAAGTCGTCCGGAGTTGCTTGTGTGAACTTGCTAGAGCAGGTTATGTGGTTTCGTCCAGAAGAAAAACACCACATAAAAAGAGAAGAATTAAGAGAAAAATGACAATGATTAGGTGCTTTTCATTACACAAGATTAAGTACACAAATAAAATTACCCTATTTTGATAATTTTATTTGTATGTGATGAAGAGATGAGGAGTAGTAGAAAGCACCTAAGCTTTTTATATAAAAGATAAATATATTTTGAGAAGTGATGCAGTATTACTCGTTAAGAAGAGACAAAAGCAAAAGCACTTAAGTCCATTGTTGGAAAAGAGTTAAAAGCAATTTGTGGCAGCTTTAAATTGGGTGTAGCAAGGGCTACACAAATAAACGACTGAATGTAGCCCAATTACACCTAATTGTCTTTTTTTCTAAAAGTGAAATGAGGACAGTTTAATATAATTGTTTTGGGTGAGGTCAAAATTCTTAAATTTTAAAAAGCAAAAAATCATTTGGGCCTGAAAATTGGGTTTTCTTTAATCAATTCTCTCTAATTTAATTCTTTTGAAATTTCTCAAAAAAAAAAATTTACTTAATTTACTGAACCAATTATGTAACCACAACACAGGGGATGGCTTCAATGACACTAACGGCATGGTTGCCGCAGCTACATCCACCGGGGTGTGGTGGATCAGTGAAATGCTTGACGCCGACGGCTTCTCAATTGGGATTCTTAATAATGAGTCTTTGTTTAGTATCAATCGGAAGCGGCGGAATACGACCGTGTAGTATTCCATTCGGGGTGGATCAATTTGATCCGACGACGGAGAAAGGGCGGAAAGGGATAGCGAGTTTTTATAATTGGTATTACGCAACATTCATGGTGGTTTTGGTGATTACATTGACAATTGTTGTGTATATTCAAGACTCAGTTAGCTGGGTTTTGGGATATGGAATTCCGACGGTGCTGATGTTTTGTTCTATAATTCTGTTCTTCATGGGAACTCATGTTTATGTGTTCATAAAGCCTGAAGGAAGCATATTTACTGGCTTAGCTCAAGTGGCTGTTGCTGCTTACAAAAAGCGTCATCTTAAACTCCCTGATGATAGGAAGGGTTTTTATGACCCCCCGTTGGACCCTGCCTCTGTTGTCCTCTCAAAGCTTCCTCTCACTAACCAATTCAGGTTCATTCCTCTCTCTCTCATTCTTTTAACTTAGTTTATTTTAAGTTAGCTATGGAGGGAGGAAGGTAAGCGTAGAAGTTATGTTAATGGTTACTAATTTAAACTTTCGAATTGAGATGTATTTATTGTAGTACTAAAGTTAAAGTAAAATACACTATGTTCTAACTTTGTAGTGTGAAAGAAATAATTTCTCTAGTCTATCGATTTCTTTTAAAAGAATAGTATATGCTAATTATTCACAATATACACTTGTAGGGAATGAAAAACAAAACAAATTGACACATTTCATAAAATAAAACAAAAACTGATGTCCCCTGGAGAGTTGATGGAATTCTTCTTGATTGACCACCTTTAAGGAAATGACATGCAACTATTCATAACTCATTTTATAACTCTATTGTTTTTATTATGAATTTTTATTTGTTTTCTCACACATCTTTTCAAAATAATTATTCATCTTTCTTATACGAGCTTTTAAAGTTGAATTTTAGAAAACCTTACTAAAATTTGAGAATATTAGAAGTAGAATTTGTATACTTTAATATTGAACTAAAAATATATTTTATGGTTAGTTTGGCGCTTCTACTATGTTCACTCTCAAATCTCTTGTAAGAATTTAGACGTTATTTGCTTTTAATTTTTGGTTTCTTTACTTTTCATTTAGTTAAACTTGTGTTTGATTTCTGTGATAATTTGCAGTTTTCTAAACAAAGCAGCAATCAAGACAGAGAATGATATAAACCCGGACGGAAGGAGAGTAAACAAATGGAATTTAAGCAGCATACAACAAGTAGAAGAATTAAAATGCTTGTTTAGAGTTTTCCCAATTTGGGTAACAGGAATCCTTAGCCTAACTCCAATTATTCAACAATCCACCTTCTCAATTTCTCAAGCTCTTAAAATGAACCGTCACATGGGGTCCAATTTCCAAATCCCTCCTGCTTCCATAATCGTCATCTCTTTCCTCACCATCACTTTCTTCATCCCTTTCTACGATCAATTCCTCGTCCCGACTCTCCGTAAATTCACTGGACACCCGAACGGCATAACCGAGCTCCAACGCATGGCCATCGGCATAGTCTTCGCAGTCCTTTCCATGGTCGTCGCTGGCCTAATCGAAATGCAGAGAAGGAATCAAGCCAACAATAACGGGTCTATAATGTCAGTTTTTTGGCTCACTCCACAATTCTTCCTAATGGGGCTCTGTGAAGCGTTTAATATAATTGGGCAAATAGAGTTTTTCAATAAGGAATTTCCAGAGCATATGAGGACTATGGGAAATGCTTTTTCGTCATGCTCTATCGCTTTATCTAGCTATATTAATACGGCTATGGTTTTGATTGTGCATCGAACCACCGGACGTGGCGGCGCTCATGGCAAGCCCGATTGGTTGATAGATGATCTCAATAAAGGGAGACTAGATTACTTTTATTATGTAGTAGCTGCAACAGCGTTTTTTAATTTTTTCTTTTTTCTTTATTGTGCTAAAAATTACCGTTACAAAGGTCATGTGTCTGACGACGAGCAAGATTTGAAACTTGTTATTAGCTCGGACAAAGTGTTGGATGTTTGAATATCATCATTTGCTTAATAATTAATTTCTTATACTTATACTCTCTATAATATAATCCTTAGTTTCACATCAAATATAC

mRNA sequence

ATGGAGAGTGAGAGTGAAAGAAAAGCTTCGAGCAATTCTCGTAGTCCTAACAACTCCAAAGATGCTAATAATAATCCCAAGCTTTCAATAATATCCAACAAATCTAAAAGAGAGCCTGGAGGATGGAAGAGCATGCCTTACATTCTTGGAAATGAAACATTTGAAAGACTAGCTGCAATGGGATTACTAGCAAATTTCATGGTGTATCTAAAAAAAATGTATCATATGGATCAAGTTTCGGCGACCAGCTTAATGGGGTTATGGACGGGAGTCACCAGTTTCTTGCCTCTTCTCGGCGCATTTCTCTCCGACGCTTATATTGGCCGCTACTGGACTATCGCTATTGCTTCCATTTTTTCCTTTCTGGGGATGGCTTCAATGACACTAACGGCATGGTTGCCGCAGCTACATCCACCGGGGTGTGGTGGATCAGTGAAATGCTTGACGCCGACGGCTTCTCAATTGGGATTCTTAATAATGAGTCTTTGTTTAGTATCAATCGGAAGCGGCGGAATACGACCGTGTAGTATTCCATTCGGGGTGGATCAATTTGATCCGACGACGGAGAAAGGGCGGAAAGGGATAGCGAGTTTTTATAATTGGTATTACGCAACATTCATGGTGGTTTTGGTGATTACATTGACAATTGTTGTGTATATTCAAGACTCAGTTAGCTGGGTTTTGGGATATGGAATTCCGACGGTGCTGATGTTTTGTTCTATAATTCTGTTCTTCATGGGAACTCATGTTTATGTGTTCATAAAGCCTGAAGGAAGCATATTTACTGGCTTAGCTCAAGTGGCTGTTGCTGCTTACAAAAAGCGTCATCTTAAACTCCCTGATGATAGGAAGGGTTTTTATGACCCCCCGTTGGACCCTGCCTCTGTTGTCCTCTCAAAGCTTCCTCTCACTAACCAATTCAGTTTTCTAAACAAAGCAGCAATCAAGACAGAGAATGATATAAACCCGGACGGAAGGAGAGTAAACAAATGGAATTTAAGCAGCATACAACAAGTAGAAGAATTAAAATGCTTGTTTAGAGTTTTCCCAATTTGGGTAACAGGAATCCTTAGCCTAACTCCAATTATTCAACAATCCACCTTCTCAATTTCTCAAGCTCTTAAAATGAACCGTCACATGGGGTCCAATTTCCAAATCCCTCCTGCTTCCATAATCGTCATCTCTTTCCTCACCATCACTTTCTTCATCCCTTTCTACGATCAATTCCTCGTCCCGACTCTCCGTAAATTCACTGGACACCCGAACGGCATAACCGAGCTCCAACGCATGGCCATCGGCATAGTCTTCGCAGTCCTTTCCATGGTCGTCGCTGGCCTAATCGAAATGCAGAGAAGGAATCAAGCCAACAATAACGGGTCTATAATGTCAGTTTTTTGGCTCACTCCACAATTCTTCCTAATGGGGCTCTGTGAAGCGTTTAATATAATTGGGCAAATAGAGTTTTTCAATAAGGAATTTCCAGAGCATATGAGGACTATGGGAAATGCTTTTTCGTCATGCTCTATCGCTTTATCTAGCTATATTAATACGGCTATGGTTTTGATTGTGCATCGAACCACCGGACGTGGCGGCGCTCATGGCAAGCCCGATTGGTTGATAGATGATCTCAATAAAGGGAGACTAGATTACTTTTATTATGTAGTAGCTGCAACAGCGTTTTTTAATTTTTTCTTTTTTCTTTATTGTGCTAAAAATTACCGTTACAAAGGTCATGTGTCTGACGACGAGCAAGATTTGAAACTTGTTATTAGCTCGGACAAAGTGTTGGATGTTTGA

Coding sequence (CDS)

ATGGAGAGTGAGAGTGAAAGAAAAGCTTCGAGCAATTCTCGTAGTCCTAACAACTCCAAAGATGCTAATAATAATCCCAAGCTTTCAATAATATCCAACAAATCTAAAAGAGAGCCTGGAGGATGGAAGAGCATGCCTTACATTCTTGGAAATGAAACATTTGAAAGACTAGCTGCAATGGGATTACTAGCAAATTTCATGGTGTATCTAAAAAAAATGTATCATATGGATCAAGTTTCGGCGACCAGCTTAATGGGGTTATGGACGGGAGTCACCAGTTTCTTGCCTCTTCTCGGCGCATTTCTCTCCGACGCTTATATTGGCCGCTACTGGACTATCGCTATTGCTTCCATTTTTTCCTTTCTGGGGATGGCTTCAATGACACTAACGGCATGGTTGCCGCAGCTACATCCACCGGGGTGTGGTGGATCAGTGAAATGCTTGACGCCGACGGCTTCTCAATTGGGATTCTTAATAATGAGTCTTTGTTTAGTATCAATCGGAAGCGGCGGAATACGACCGTGTAGTATTCCATTCGGGGTGGATCAATTTGATCCGACGACGGAGAAAGGGCGGAAAGGGATAGCGAGTTTTTATAATTGGTATTACGCAACATTCATGGTGGTTTTGGTGATTACATTGACAATTGTTGTGTATATTCAAGACTCAGTTAGCTGGGTTTTGGGATATGGAATTCCGACGGTGCTGATGTTTTGTTCTATAATTCTGTTCTTCATGGGAACTCATGTTTATGTGTTCATAAAGCCTGAAGGAAGCATATTTACTGGCTTAGCTCAAGTGGCTGTTGCTGCTTACAAAAAGCGTCATCTTAAACTCCCTGATGATAGGAAGGGTTTTTATGACCCCCCGTTGGACCCTGCCTCTGTTGTCCTCTCAAAGCTTCCTCTCACTAACCAATTCAGTTTTCTAAACAAAGCAGCAATCAAGACAGAGAATGATATAAACCCGGACGGAAGGAGAGTAAACAAATGGAATTTAAGCAGCATACAACAAGTAGAAGAATTAAAATGCTTGTTTAGAGTTTTCCCAATTTGGGTAACAGGAATCCTTAGCCTAACTCCAATTATTCAACAATCCACCTTCTCAATTTCTCAAGCTCTTAAAATGAACCGTCACATGGGGTCCAATTTCCAAATCCCTCCTGCTTCCATAATCGTCATCTCTTTCCTCACCATCACTTTCTTCATCCCTTTCTACGATCAATTCCTCGTCCCGACTCTCCGTAAATTCACTGGACACCCGAACGGCATAACCGAGCTCCAACGCATGGCCATCGGCATAGTCTTCGCAGTCCTTTCCATGGTCGTCGCTGGCCTAATCGAAATGCAGAGAAGGAATCAAGCCAACAATAACGGGTCTATAATGTCAGTTTTTTGGCTCACTCCACAATTCTTCCTAATGGGGCTCTGTGAAGCGTTTAATATAATTGGGCAAATAGAGTTTTTCAATAAGGAATTTCCAGAGCATATGAGGACTATGGGAAATGCTTTTTCGTCATGCTCTATCGCTTTATCTAGCTATATTAATACGGCTATGGTTTTGATTGTGCATCGAACCACCGGACGTGGCGGCGCTCATGGCAAGCCCGATTGGTTGATAGATGATCTCAATAAAGGGAGACTAGATTACTTTTATTATGTAGTAGCTGCAACAGCGTTTTTTAATTTTTTCTTTTTTCTTTATTGTGCTAAAAATTACCGTTACAAAGGTCATGTGTCTGACGACGAGCAAGATTTGAAACTTGTTATTAGCTCGGACAAAGTGTTGGATGTTTGA

Protein sequence

MESESERKASSNSRSPNNSKDANNNPKLSIISNKSKREPGGWKSMPYILGNETFERLAAMGLLANFMVYLKKMYHMDQVSATSLMGLWTGVTSFLPLLGAFLSDAYIGRYWTIAIASIFSFLGMASMTLTAWLPQLHPPGCGGSVKCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPFGVDQFDPTTEKGRKGIASFYNWYYATFMVVLVITLTIVVYIQDSVSWVLGYGIPTVLMFCSIILFFMGTHVYVFIKPEGSIFTGLAQVAVAAYKKRHLKLPDDRKGFYDPPLDPASVVLSKLPLTNQFSFLNKAAIKTENDINPDGRRVNKWNLSSIQQVEELKCLFRVFPIWVTGILSLTPIIQQSTFSISQALKMNRHMGSNFQIPPASIIVISFLTITFFIPFYDQFLVPTLRKFTGHPNGITELQRMAIGIVFAVLSMVVAGLIEMQRRNQANNNGSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPEHMRTMGNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWLIDDLNKGRLDYFYYVVAATAFFNFFFFLYCAKNYRYKGHVSDDEQDLKLVISSDKVLDV*
Homology
BLAST of CsaV3_5G032220.1 vs. NCBI nr
Match: XP_004135202.1 (protein NRT1/ PTR FAMILY 2.13 [Cucumis sativus] >KGN51872.1 hypothetical protein Csa_008529 [Cucumis sativus])

HSP 1 Score: 1191.0 bits (3080), Expect = 0.0e+00
Identity = 598/598 (100.00%), Postives = 598/598 (100.00%), Query Frame = 0

Query: 1   MESESERKASSNSRSPNNSKDANNNPKLSIISNKSKREPGGWKSMPYILGNETFERLAAM 60
           MESESERKASSNSRSPNNSKDANNNPKLSIISNKSKREPGGWKSMPYILGNETFERLAAM
Sbjct: 1   MESESERKASSNSRSPNNSKDANNNPKLSIISNKSKREPGGWKSMPYILGNETFERLAAM 60

Query: 61  GLLANFMVYLKKMYHMDQVSATSLMGLWTGVTSFLPLLGAFLSDAYIGRYWTIAIASIFS 120
           GLLANFMVYLKKMYHMDQVSATSLMGLWTGVTSFLPLLGAFLSDAYIGRYWTIAIASIFS
Sbjct: 61  GLLANFMVYLKKMYHMDQVSATSLMGLWTGVTSFLPLLGAFLSDAYIGRYWTIAIASIFS 120

Query: 121 FLGMASMTLTAWLPQLHPPGCGGSVKCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPFG 180
           FLGMASMTLTAWLPQLHPPGCGGSVKCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPFG
Sbjct: 121 FLGMASMTLTAWLPQLHPPGCGGSVKCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPFG 180

Query: 181 VDQFDPTTEKGRKGIASFYNWYYATFMVVLVITLTIVVYIQDSVSWVLGYGIPTVLMFCS 240
           VDQFDPTTEKGRKGIASFYNWYYATFMVVLVITLTIVVYIQDSVSWVLGYGIPTVLMFCS
Sbjct: 181 VDQFDPTTEKGRKGIASFYNWYYATFMVVLVITLTIVVYIQDSVSWVLGYGIPTVLMFCS 240

Query: 241 IILFFMGTHVYVFIKPEGSIFTGLAQVAVAAYKKRHLKLPDDRKGFYDPPLDPASVVLSK 300
           IILFFMGTHVYVFIKPEGSIFTGLAQVAVAAYKKRHLKLPDDRKGFYDPPLDPASVVLSK
Sbjct: 241 IILFFMGTHVYVFIKPEGSIFTGLAQVAVAAYKKRHLKLPDDRKGFYDPPLDPASVVLSK 300

Query: 301 LPLTNQFSFLNKAAIKTENDINPDGRRVNKWNLSSIQQVEELKCLFRVFPIWVTGILSLT 360
           LPLTNQFSFLNKAAIKTENDINPDGRRVNKWNLSSIQQVEELKCLFRVFPIWVTGILSLT
Sbjct: 301 LPLTNQFSFLNKAAIKTENDINPDGRRVNKWNLSSIQQVEELKCLFRVFPIWVTGILSLT 360

Query: 361 PIIQQSTFSISQALKMNRHMGSNFQIPPASIIVISFLTITFFIPFYDQFLVPTLRKFTGH 420
           PIIQQSTFSISQALKMNRHMGSNFQIPPASIIVISFLTITFFIPFYDQFLVPTLRKFTGH
Sbjct: 361 PIIQQSTFSISQALKMNRHMGSNFQIPPASIIVISFLTITFFIPFYDQFLVPTLRKFTGH 420

Query: 421 PNGITELQRMAIGIVFAVLSMVVAGLIEMQRRNQANNNGSIMSVFWLTPQFFLMGLCEAF 480
           PNGITELQRMAIGIVFAVLSMVVAGLIEMQRRNQANNNGSIMSVFWLTPQFFLMGLCEAF
Sbjct: 421 PNGITELQRMAIGIVFAVLSMVVAGLIEMQRRNQANNNGSIMSVFWLTPQFFLMGLCEAF 480

Query: 481 NIIGQIEFFNKEFPEHMRTMGNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWLI 540
           NIIGQIEFFNKEFPEHMRTMGNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWLI
Sbjct: 481 NIIGQIEFFNKEFPEHMRTMGNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWLI 540

Query: 541 DDLNKGRLDYFYYVVAATAFFNFFFFLYCAKNYRYKGHVSDDEQDLKLVISSDKVLDV 599
           DDLNKGRLDYFYYVVAATAFFNFFFFLYCAKNYRYKGHVSDDEQDLKLVISSDKVLDV
Sbjct: 541 DDLNKGRLDYFYYVVAATAFFNFFFFLYCAKNYRYKGHVSDDEQDLKLVISSDKVLDV 598

BLAST of CsaV3_5G032220.1 vs. NCBI nr
Match: XP_008446297.2 (PREDICTED: LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 2.13-like [Cucumis melo])

HSP 1 Score: 1131.3 bits (2925), Expect = 0.0e+00
Identity = 569/599 (94.99%), Postives = 583/599 (97.33%), Query Frame = 0

Query: 1   MESESERKASSNSRSPNNSKDA-NNNPKLSIISNKSKREPGGWKSMPYILGNETFERLAA 60
           MESESE+K SSNS SPNN KDA NNNPKLS ISNKSKREPGGWKSMPYILGNETFERLA+
Sbjct: 1   MESESEKKGSSNSHSPNNFKDANNNNPKLS-ISNKSKREPGGWKSMPYILGNETFERLAS 60

Query: 61  MGLLANFMVYLKKMYHMDQVSATSLMGLWTGVTSFLPLLGAFLSDAYIGRYWTIAIASIF 120
           MGLLANFMVYLK +YHMDQVSATSLMGLWTGVTSFLPLLGAFLSDAYIGRYWTIAIASIF
Sbjct: 61  MGLLANFMVYLKTVYHMDQVSATSLMGLWTGVTSFLPLLGAFLSDAYIGRYWTIAIASIF 120

Query: 121 SFLGMASMTLTAWLPQLHPPGCGGSVKCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPF 180
           SFLGMASMTLTAWLPQLHPPGCGGSVKCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPF
Sbjct: 121 SFLGMASMTLTAWLPQLHPPGCGGSVKCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPF 180

Query: 181 GVDQFDPTTEKGRKGIASFYNWYYATFMVVLVITLTIVVYIQDSVSWVLGYGIPTVLMFC 240
           GVDQFDPTTEKGRKGIASFYNWYYATFMVVLV TLTIVVYIQDSVSWVLGYGIPT LMFC
Sbjct: 181 GVDQFDPTTEKGRKGIASFYNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGLMFC 240

Query: 241 SIILFFMGTHVYVFIKPEGSIFTGLAQVAVAAYKKRHLKLPDDRKGFYDPPLDPASVVLS 300
           SIILFFMGTHVYVFIKPEGSIFTGLAQVAVAAYKKRHLKLPDDRKGFYDPPLDPASVVLS
Sbjct: 241 SIILFFMGTHVYVFIKPEGSIFTGLAQVAVAAYKKRHLKLPDDRKGFYDPPLDPASVVLS 300

Query: 301 KLPLTNQFSFLNKAAIKTENDINPDGRRVNKWNLSSIQQVEELKCLFRVFPIWVTGILSL 360
           KLPLTNQFSFLNKAAI TEN+I PDG R+NKWNLSSIQQVEELKCLFRVFPIWVTGILSL
Sbjct: 301 KLPLTNQFSFLNKAAIVTENEIKPDGTRLNKWNLSSIQQVEELKCLFRVFPIWVTGILSL 360

Query: 361 TPIIQQSTFSISQALKMNRHMGSNFQIPPASIIVISFLTITFFIPFYDQFLVPTLRKFTG 420
           TPIIQQSTFSISQAL+MNRHMGSNFQIPPASIIVISFLTITFFIP YD+FLVPTLRKFTG
Sbjct: 361 TPIIQQSTFSISQALQMNRHMGSNFQIPPASIIVISFLTITFFIPLYDRFLVPTLRKFTG 420

Query: 421 HPNGITELQRMAIGIVFAVLSMVVAGLIEMQRRNQANNNGSIMSVFWLTPQFFLMGLCEA 480
           HPNGITELQRMAIGI+FAVLSMVVAGL+EMQRRNQANN+GSIMSVFWLTPQFFLMGLCEA
Sbjct: 421 HPNGITELQRMAIGIIFAVLSMVVAGLVEMQRRNQANNDGSIMSVFWLTPQFFLMGLCEA 480

Query: 481 FNIIGQIEFFNKEFPEHMRTMGNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWL 540
           FNIIGQIEFFNKEFPEHMRTMGNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWL
Sbjct: 481 FNIIGQIEFFNKEFPEHMRTMGNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWL 540

Query: 541 IDDLNKGRLDYFYYVVAATAFFNFFFFLYCAKNYRYKGHVSDDEQDLKLVISSDKVLDV 599
           IDDLNKGRLDYFYY+VAATA F  F FLYCAKNYRYKGHVSDDEQDL+LV+SSDK+LDV
Sbjct: 541 IDDLNKGRLDYFYYIVAATAXFVIFLFLYCAKNYRYKGHVSDDEQDLQLVVSSDKLLDV 598

BLAST of CsaV3_5G032220.1 vs. NCBI nr
Match: XP_038892122.1 (protein NRT1/ PTR FAMILY 2.13-like [Benincasa hispida])

HSP 1 Score: 1071.2 bits (2769), Expect = 3.2e-309
Identity = 542/599 (90.48%), Postives = 564/599 (94.16%), Query Frame = 0

Query: 1   MESESERKASSNSRSPNNSKDANNNPKLSIISNKSKREPGGWKSMPYILGNETFERLAAM 60
           MESE+E KASS   SP+N +    +  L    NKSKREPGGWKSMPYILGNETFERLA+M
Sbjct: 15  MESEAESKASS---SPSNLQCFTKDVSL---HNKSKREPGGWKSMPYILGNETFERLASM 74

Query: 61  GLLANFMVYLKKMYHMDQVSATSLMGLWTGVTSFLPLLGAFLSDAYIGRYWTIAIASIFS 120
           GLLANFMVYLKK+YHMDQVSATSLMGLWTGVTSFLPLLGAFLSDAYIGRYWTIA+ASIFS
Sbjct: 75  GLLANFMVYLKKVYHMDQVSATSLMGLWTGVTSFLPLLGAFLSDAYIGRYWTIAVASIFS 134

Query: 121 FLGMASMTLTAWLPQLHPPGCGGSVKCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPFG 180
           FLGM SMTLTAWLPQLHP GC GS KC  PTASQLGFLIMSLCLVSIGSGGIRPCSIPFG
Sbjct: 135 FLGMTSMTLTAWLPQLHPQGCVGSTKCSGPTASQLGFLIMSLCLVSIGSGGIRPCSIPFG 194

Query: 181 VDQFDPTTEKGRKGIASFYNWYYATFMVVLVITLTIVVYIQDSVSWVLGYGIPTVLMFCS 240
           VDQFDPTTEKGRKGIASFYNWYYATFMVVLV TLTIVVYIQDSVSWVLGYGIPT LMFCS
Sbjct: 195 VDQFDPTTEKGRKGIASFYNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGLMFCS 254

Query: 241 IILFFMGTHVYVFIKPEGSIFTGLAQVAVAAYKKRHLKLPDDRKGFYDPPLDPASVVLSK 300
           I+LFFMGT VYVFIKPEGSIFTGLAQVAVAAYKKRHLKLPDDRKGFYDPPLDP SVVLSK
Sbjct: 255 IVLFFMGTRVYVFIKPEGSIFTGLAQVAVAAYKKRHLKLPDDRKGFYDPPLDPDSVVLSK 314

Query: 301 LPLTNQFSFLNKAAIKTENDINPDGRRVNKWNLSSIQQVEELKCLFRVFPIWVTGILSLT 360
           LPLTNQFSFLNKAAI TE +INPDG RVNKWN+SSIQQVEELKCLFRVFPIWVTGILSLT
Sbjct: 315 LPLTNQFSFLNKAAIITEKEINPDGTRVNKWNVSSIQQVEELKCLFRVFPIWVTGILSLT 374

Query: 361 PIIQQSTFSISQALKMNRHMGSNFQIPPASIIVISFLTITFFIPFYDQFLVPTLRKFTGH 420
           PIIQQSTFSISQAL+MNRHMG NFQIPPASIIVISFLTITFFIPFYDQFLVPTLRKFT H
Sbjct: 375 PIIQQSTFSISQALQMNRHMGPNFQIPPASIIVISFLTITFFIPFYDQFLVPTLRKFTRH 434

Query: 421 PNGITELQRMAIGIVFAVLSMVVAGLIEMQRRNQANNNGSIMSVFWLTPQFFLMGLCEAF 480
           PNGITELQRMA+GI+FAV+SMVVAGLIEMQRRN+ANN GSIMSVFWLTPQFFLMGLCEAF
Sbjct: 435 PNGITELQRMAVGIIFAVVSMVVAGLIEMQRRNRANNGGSIMSVFWLTPQFFLMGLCEAF 494

Query: 481 NIIGQIEFFNKEFPEHMRTMGNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWLI 540
           NIIGQIEFFNKEFPEHMRTMGNAFSSCSIALSSY+NTAMVLIVHRTTG GGAHG+PDWL+
Sbjct: 495 NIIGQIEFFNKEFPEHMRTMGNAFSSCSIALSSYVNTAMVLIVHRTTGSGGAHGRPDWLV 554

Query: 541 DDLNKGRLDYFYYVVAATAFFNFFFFLYCAKNYRYKGHVSDDEQDLKLVISSD-KVLDV 599
           DDLNKGRLDYFYYVVAATAF NFFFFLYCAKNYRYKGHVS+ E+DL+LVISSD K+LDV
Sbjct: 555 DDLNKGRLDYFYYVVAATAFCNFFFFLYCAKNYRYKGHVSNTERDLELVISSDKKILDV 607

BLAST of CsaV3_5G032220.1 vs. NCBI nr
Match: KAA0034378.1 (protein NRT1/ PTR FAMILY 2.13-like [Cucumis melo var. makuwa] >TYK15541.1 protein NRT1/ PTR FAMILY 2.13-like [Cucumis melo var. makuwa])

HSP 1 Score: 1023.5 bits (2645), Expect = 7.5e-295
Identity = 505/523 (96.56%), Postives = 516/523 (98.66%), Query Frame = 0

Query: 76  MDQVSATSLMGLWTGVTSFLPLLGAFLSDAYIGRYWTIAIASIFSFLGMASMTLTAWLPQ 135
           MDQVSATSLMGLWTGVTSFLPLLGAFLSDAYIGRYWTIAIASIFSFLGMASMTLTAWLPQ
Sbjct: 1   MDQVSATSLMGLWTGVTSFLPLLGAFLSDAYIGRYWTIAIASIFSFLGMASMTLTAWLPQ 60

Query: 136 LHPPGCGGSVKCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPFGVDQFDPTTEKGRKGI 195
           LHPPGCGGSVKCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPFGVDQFDPTTEKGRKGI
Sbjct: 61  LHPPGCGGSVKCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPFGVDQFDPTTEKGRKGI 120

Query: 196 ASFYNWYYATFMVVLVITLTIVVYIQDSVSWVLGYGIPTVLMFCSIILFFMGTHVYVFIK 255
           ASFYNWYYATFMVVLV TLTIVVYIQDSVSWVLGYGIPT LMFCSIILFFMGTHVYVFIK
Sbjct: 121 ASFYNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGLMFCSIILFFMGTHVYVFIK 180

Query: 256 PEGSIFTGLAQVAVAAYKKRHLKLPDDRKGFYDPPLDPASVVLSKLPLTNQFSFLNKAAI 315
           PEGSIFTGLAQVAVAAYKKRHLKLPDDRKGFYDPPLDPASVVLSKLPLTNQFSFLNKAAI
Sbjct: 181 PEGSIFTGLAQVAVAAYKKRHLKLPDDRKGFYDPPLDPASVVLSKLPLTNQFSFLNKAAI 240

Query: 316 KTENDINPDGRRVNKWNLSSIQQVEELKCLFRVFPIWVTGILSLTPIIQQSTFSISQALK 375
            TEN+I PDG R+NKWNLSSIQQVEELKCLFRVFPIWVTGILSLTPIIQQSTFSISQAL+
Sbjct: 241 VTENEIKPDGTRLNKWNLSSIQQVEELKCLFRVFPIWVTGILSLTPIIQQSTFSISQALQ 300

Query: 376 MNRHMGSNFQIPPASIIVISFLTITFFIPFYDQFLVPTLRKFTGHPNGITELQRMAIGIV 435
           MNRHMGSNFQIPPASIIVISFLTITFFIP YD+FLVPTLRKFTGHPNGITELQRMAIGI+
Sbjct: 301 MNRHMGSNFQIPPASIIVISFLTITFFIPLYDRFLVPTLRKFTGHPNGITELQRMAIGII 360

Query: 436 FAVLSMVVAGLIEMQRRNQANNNGSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPE 495
           FAVLSMVVAGL+EMQRRNQANN+GSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPE
Sbjct: 361 FAVLSMVVAGLVEMQRRNQANNDGSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPE 420

Query: 496 HMRTMGNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWLIDDLNKGRLDYFYYVV 555
           HMRTMGNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWLIDDLNKGRLDYFYY+V
Sbjct: 421 HMRTMGNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWLIDDLNKGRLDYFYYIV 480

Query: 556 AATAFFNFFFFLYCAKNYRYKGHVSDDEQDLKLVISSDKVLDV 599
           AATAF NFFFFLYCAKNYRYKGHVSDDEQDL+LV+SSDK+LDV
Sbjct: 481 AATAFCNFFFFLYCAKNYRYKGHVSDDEQDLQLVVSSDKLLDV 523

BLAST of CsaV3_5G032220.1 vs. NCBI nr
Match: XP_022998476.1 (protein NRT1/ PTR FAMILY 2.13-like [Cucurbita maxima])

HSP 1 Score: 1015.8 bits (2625), Expect = 1.6e-292
Identity = 504/579 (87.05%), Postives = 535/579 (92.40%), Query Frame = 0

Query: 25  NPKLSII-SNKSKREPGGWKSMPYILGNETFERLAAMGLLANFMVYLKKMYHMDQVSATS 84
           NPKL     NK+KREPGGWKSMPYILGNETFERLA+MGLLANFMVYL K+YHMDQVSATS
Sbjct: 10  NPKLCFSPQNKAKREPGGWKSMPYILGNETFERLASMGLLANFMVYLTKVYHMDQVSATS 69

Query: 85  LMGLWTGVTSFLPLLGAFLSDAYIGRYWTIAIASIFSFLGMASMTLTAWLPQLHPPGCGG 144
           LMGLWTGVT+FLPLLGAFLSDAY GRYWTIA+AS FSFLGM SMTLTAWLPQLHPP C G
Sbjct: 70  LMGLWTGVTNFLPLLGAFLSDAYAGRYWTIAVASFFSFLGMTSMTLTAWLPQLHPPTCSG 129

Query: 145 SVKCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPFGVDQFDPTTEKGRKGIASFYNWYY 204
           +  C  PTASQLGFL+MSLCLVSIGS GIRPCSIPFGVDQFDPTT+KGRKGIASFYNWYY
Sbjct: 130 A-NCEGPTASQLGFLVMSLCLVSIGSAGIRPCSIPFGVDQFDPTTDKGRKGIASFYNWYY 189

Query: 205 ATFMVVLVITLTIVVYIQDSVSWVLGYGIPTVLMFCSIILFFMGTHVYVFIKPEGSIFTG 264
           ATF VVLV TLTIVVYIQDSVSWVLGYGIPTVLMFCSI+LFFMGTHVYVFIKPEGSIFTG
Sbjct: 190 ATFSVVLVFTLTIVVYIQDSVSWVLGYGIPTVLMFCSIVLFFMGTHVYVFIKPEGSIFTG 249

Query: 265 LAQVAVAAYKKRHLKLP--DDRKGFYDPPLDPASVVLSKLPLTNQFSFLNKAAIKTENDI 324
           LAQVAVAAYKKRHL+LP  DDR  FYDPPL+PASVVLSKLPLTNQFSFL KAAI  E ++
Sbjct: 250 LAQVAVAAYKKRHLELPSLDDRTAFYDPPLEPASVVLSKLPLTNQFSFLTKAAIVVEKEL 309

Query: 325 NPDGRRVNKWNLSSIQQVEELKCLFRVFPIWVTGILSLTPIIQQSTFSISQALKMNRHMG 384
           NPDG R+NKWNLSSIQQVEELKCL RV PIWVTGILSLTPIIQQ+TFSISQAL+M+RHMG
Sbjct: 310 NPDGTRINKWNLSSIQQVEELKCLVRVGPIWVTGILSLTPIIQQATFSISQALQMDRHMG 369

Query: 385 SNFQIPPASIIVISFLTITFFIPFYDQFLVPTLRKFTGHPNGITELQRMAIGIVFAVLSM 444
            NFQ+PPASIIVISFLTITFFIP YDQFLVP  RKFT HPNGITELQRMA+GI+FAVLSM
Sbjct: 370 PNFQMPPASIIVISFLTITFFIPLYDQFLVPAFRKFTQHPNGITELQRMAVGIIFAVLSM 429

Query: 445 VVAGLIEMQRRNQAN--NNGSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPEHMRT 504
           +VAG++E +RRN+AN  N GSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPEHMRT
Sbjct: 430 IVAGIVEKERRNRANGPNGGSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPEHMRT 489

Query: 505 MGNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWLIDDLNKGRLDYFYYVVAATA 564
           MGNAFSSCSIALSSY+NTAMVLIVHRTTG GGAHGKPDWL +DLNKGRLDYFYYVVAATA
Sbjct: 490 MGNAFSSCSIALSSYVNTAMVLIVHRTTGSGGAHGKPDWLTNDLNKGRLDYFYYVVAATA 549

Query: 565 FFNFFFFLYCAKNYRYKGHVSDDEQDLKLVISSDKVLDV 599
           FFNFFFF+YCAKNY YKG VSD +QDL+LVI+SDK LDV
Sbjct: 550 FFNFFFFIYCAKNYHYKGKVSDSQQDLELVIASDKKLDV 587

BLAST of CsaV3_5G032220.1 vs. ExPASy Swiss-Prot
Match: Q8RX77 (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 4.1e-179
Identity = 309/574 (53.83%), Postives = 419/574 (73.00%), Query Frame = 0

Query: 24  NNPKLSIISNKSKREPGGWKSMPYILGNETFERLAAMGLLANFMVYLKKMYHMDQVSATS 83
           ++P   + + K +++PGGW+++ +ILGNET ERL ++GLLANFMVYL K++H++QV A +
Sbjct: 37  SSPGSILDAEKVEKKPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAAN 96

Query: 84  LMGLWTGVTSFLPLLGAFLSDAYIGRYWTIAIASIFSFLGMASMTLTAWLPQLHPPGCGG 143
           ++ +W+G T+  PL+GA++SD Y+GR+ TIA AS  + LG+ ++TLTA  PQLHP  C  
Sbjct: 97  VINIWSGFTNLTPLVGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNS 156

Query: 144 S--VKCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPFGVDQFDPTTEKGRKGIASFYNW 203
              + C  P   Q+G L++ LC +S+GSGGIRPCSIPFGVDQFD  TE+G KG+ASF+NW
Sbjct: 157 QDPLSCGGPNKLQIGVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNW 216

Query: 204 YYATFMVVLVITLTIVVYIQDSVSWVLGYGIPTVLMFCSIILFFMGTHVYVFIKPEGSIF 263
           YY TF VVL+IT T+VVYIQD VSW++G+ IPT LM  ++++FF G   YV++KPEGSIF
Sbjct: 217 YYMTFTVVLIITQTVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIF 276

Query: 264 TGLAQVAVAAYKKRHLKLPDDRKG---FYDPPLDPASVVLSKLPLTNQFSFLNKAAIKTE 323
           +G+AQV VAA KKR LKLP +  G   +YDP +   S VLSKL  +NQF  L+KAA+  E
Sbjct: 277 SGIAQVIVAARKKRKLKLPAEDDGTVTYYDPAI--KSSVLSKLHRSNQFRCLDKAAVVIE 336

Query: 324 NDINPDGRRVNKWNLSSIQQVEELKCLFRVFPIWVTGILSLTPIIQQSTFSISQALKMNR 383
            D+ P+G   +KW L S+Q+VEE+KCL R+ PIW  GI+SL  +  Q TF++SQALKM+R
Sbjct: 337 GDLTPEGPPADKWRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDR 396

Query: 384 HMGSNFQIPPASIIVISFLTITFFIPFYDQFLVPTLRKFTGHPNGITELQRMAIGIVFAV 443
           ++G  F+IP  S+ VIS LTI  F+PFYD+  VP +R+ TGH +GIT LQR+  GIVFA+
Sbjct: 397 NLGPKFEIPAGSLSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAI 456

Query: 444 LSMVVAGLIEMQRRNQANNNG-----SIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEF 503
            SM+VAG++E  RR ++ N G     + MSVFWL+PQ  LMGLCEAFNIIGQIEFFN +F
Sbjct: 457 FSMIVAGIVERMRRIRSINAGDPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQF 516

Query: 504 PEHMRTMGNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWLIDDLNKGRLDYFYY 563
           PEHMR++ N+  S S A SSY+++ +V +VH+ +   G H +PDWL  +LN G+LDYFYY
Sbjct: 517 PEHMRSIANSLFSLSFAGSSYLSSFLVTVVHKFS---GGHDRPDWLNKNLNAGKLDYFYY 576

Query: 564 VVAATAFFNFFFFLYCAKNYRYKG--HVSDDEQD 586
           ++A     N  +F YCA+ YRYK    + D E+D
Sbjct: 577 LIAVLGVVNLVYFWYCARGYRYKVGLPIEDFEED 605

BLAST of CsaV3_5G032220.1 vs. ExPASy Swiss-Prot
Match: Q9LFX9 (Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana OX=3702 GN=NPF2.12 PE=1 SV=2)

HSP 1 Score: 576.6 bits (1485), Expect = 3.2e-163
Identity = 292/560 (52.14%), Postives = 398/560 (71.07%), Query Frame = 0

Query: 28  LSIISNKS---KREPGGWKSMPYILGNETFERLAAMGLLANFMVYLKKMYHMDQVSATSL 87
           + ++ N+    +++ GGW+++ +ILGNET E+L ++G+ ANFM+YL+ ++HM+ V A ++
Sbjct: 1   MGVVENRKILPEKKLGGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNV 60

Query: 88  MGLWTGVTSFLPLLGAFLSDAYIGRYWTIAIASIFSFLGMASMTLTAWLPQLHPPGCGG- 147
             LW G+T+F PLLGA +SDAYIGR+ TIA AS+FS LG+ ++TLTA LPQLHPP C   
Sbjct: 61  YYLWMGLTNFAPLLGALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNP 120

Query: 148 -SVKCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPFGVDQFDPTTEKGRKGIASFYNWY 207
              +C  P   QLG L + L  +SIGSGGIRPCSIPFGVDQFD  TE+G KG+ASF+NWY
Sbjct: 121 HPDECDDPNKLQLGILFLGLGFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVASFFNWY 180

Query: 208 YATFMVVLVITLTIVVYIQDSVSWVLGYGIPTVLMFCSIILFFMGTHVYVFIKPEGSIFT 267
           Y T  +VL+ + T+VVY+Q +VSWV+G+ IPT LM C+++LFF+G   YV++KPEGS+F+
Sbjct: 181 YLTLTMVLIFSHTVVVYLQ-TVSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFS 240

Query: 268 GLAQVAVAAYKKRHLKL---PDDRKGFYDPPLDPASVVLSKLPLTNQFSFLNKAAIKTEN 327
           G+A+V VAA KKR LK+    D  + +Y+PP+ P   VLSKLPLT+QF FL+KAA+  + 
Sbjct: 241 GIARVIVAARKKRDLKISLVDDGTEEYYEPPVKPG--VLSKLPLTDQFKFLDKAAVILDG 300

Query: 328 DINPDGRRVNKWNLSSIQQVEELKCLFRVFPIWVTGILSLTPIIQQSTFSISQALKMNRH 387
           D+  +G   NKW L SIQ+VEE+KCL RV P+W  GI+S+  +  Q+TF + QA KM+RH
Sbjct: 301 DLTSEGVPANKWRLCSIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRH 360

Query: 388 MGSNFQIPPASIIVISFLTITFFIPFYDQFLVPTL---RKFTGHPNGITELQRMAIGIVF 447
           MG +F+IP ASI VIS++TI  ++P Y+  LVP L   RKF      +T LQRM IGIVF
Sbjct: 361 MGPHFEIPAASITVISYITIGIWVPIYEHLLVPFLWRMRKFR-----VTLLQRMGIGIVF 420

Query: 448 AVLSMVVAGLIEMQRRNQANNNGSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPEH 507
           A+LSM  AG +E  RR +A    + MSVFWL     LMGLCE+FN IG IEFFN +FPEH
Sbjct: 421 AILSMFTAGFVEGVRRTRATEM-TQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEH 480

Query: 508 MRTMGNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWLIDDLNKGRLDYFYYVVA 567
           MR++ N+    S A ++Y+++ +V  VH+ +   G    PDWL  DL++G+LDYFYY++A
Sbjct: 481 MRSIANSLFPLSFAAANYLSSLLVTTVHKVS---GTKDHPDWLNKDLDRGKLDYFYYLIA 540

Query: 568 ATAFFNFFFFLYCAKNYRYK 577
                N  +F YCA  Y+YK
Sbjct: 541 VLGVVNLVYFWYCAHRYQYK 548

BLAST of CsaV3_5G032220.1 vs. ExPASy Swiss-Prot
Match: Q9CAR9 (Putative protein NRT1/ PTR FAMILY 2.14 OS=Arabidopsis thaliana OX=3702 GN=NPF2.14 PE=5 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 5.3e-142
Identity = 251/550 (45.64%), Postives = 377/550 (68.55%), Query Frame = 0

Query: 36  KREPGGWKSMPYILGNETFERLAAMGLLANFMVYLKKMYHMDQVSATSLMGLWTGVTSFL 95
           +R+P GWK+MPYI+GNET ERLA  GL+ANFMVY+ + YHMDQV A +L+  W+ +T+F 
Sbjct: 16  QRKPLGWKAMPYIIGNETLERLATFGLMANFMVYMVREYHMDQVQAVTLINTWSALTNFA 75

Query: 96  PLLGAFLSDAYIGRYWTIAIASIFSFLGMASMTLTAWLPQLHPPGCGG---SVKCLTPTA 155
           P++GAF+SD+Y G++ TI   SI   LGM  +T T+ +P L PP C     + +C+  + 
Sbjct: 76  PIIGAFISDSYTGKFNTIVFGSIAELLGMLVLTFTSLVPNLRPPPCTADQITGQCIPYSY 135

Query: 156 SQLGFLIMSLCLVSIGSGGIRPCSIPFGVDQFDPTTEKGRKGIASFYNWYYATFMVVLVI 215
           SQL  L+  L L+S+G+GGIR CS+PF +DQFD +TE+GR+G  SF++WYY T  +V ++
Sbjct: 136 SQLYVLLSGLFLLSVGTGGIRSCSVPFSLDQFDDSTEEGREGSRSFFSWYYTTHTIVQLV 195

Query: 216 TLTIVVYIQDSVSWVLGYGIPTVLMFCSIILFFMGTHVYVFIKPEGSIFTGLAQVAVAAY 275
           ++T+V+Y+Q+++SW +G+ IPTVL F +++L F+GT  YVF+KPEGS+F+G+ +V VAAY
Sbjct: 196 SMTLVLYVQNNISWGIGFAIPTVLNFFALLLLFVGTRYYVFVKPEGSVFSGVFKVLVAAY 255

Query: 276 KKRHLKLPDDRKGFYDPPLDPASVVLSKLPLTNQFSFLNKAAIKTENDINPDGRRVNKWN 335
           KKR  +        Y  PL    +  +KL LT+QF FLNKA I   ND   +     +W 
Sbjct: 256 KKRKARFTSGID--YHQPLLETDLQSNKLVLTDQFRFLNKAVIVMNNDEAGN----EEWR 315

Query: 336 LSSIQQVEELKCLFRVFPIWVTGILSLTPIIQQSTFSISQALKMN-RHMGSNFQIPPASI 395
             +++Q+E++K +  + PI+ + I+    + QQ TF++SQALKM+ +  G+++ IPPASI
Sbjct: 316 TCTVRQIEDIKSIISIIPIFASSIIGFLAMNQQQTFTVSQALKMDLQFPGTSYLIPPASI 375

Query: 396 IVISFLTITFFIPFYDQFLVPTLRKFTGHPNGITELQRMAIGIVFAVLSMVVAGLIEMQR 455
            VIS L I  ++PFY+  LV  +   T    GI+ LQ++ IG +F++ +M+++G++E +R
Sbjct: 376 TVISLLNIGIWLPFYETVLVRHIENITKQNGGISLLQKVGIGNIFSISTMLISGIVERKR 435

Query: 456 RNQANNNGSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPEHMRTMGNAFSSCSIAL 515
           R+  + NG  MSVFWLTPQ  LMG  + F I+G  EFFNK+ P +MR++GN+     ++L
Sbjct: 436 RD-LSLNGVKMSVFWLTPQQVLMGFYQVFTIVGLTEFFNKQVPINMRSIGNSLLYLGLSL 495

Query: 516 SSYINTAMVLIVHRTTGRGGAHGKPDWLIDDLNKGRLDYFYYVVAATAFFNFFFFLYCAK 575
           +SY+++AMV IVH  T RGG      WL DD++K +LD FYY +AA +  NF FF +CA+
Sbjct: 496 ASYLSSAMVSIVHSVTARGG----QSWLTDDIDKSKLDCFYYFIAALSTLNFIFFFWCAR 554

Query: 576 NYRYKGHVSD 582
            YRY+ + ++
Sbjct: 556 RYRYRNYSNE 554

BLAST of CsaV3_5G032220.1 vs. ExPASy Swiss-Prot
Match: Q9LV10 (Protein NRT1/ PTR FAMILY 2.11 OS=Arabidopsis thaliana OX=3702 GN=NPF2.11 PE=1 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 3.9e-129
Identity = 251/568 (44.19%), Postives = 351/568 (61.80%), Query Frame = 0

Query: 31  ISNKSKREPGGWKSMPYILGNETFERLAAMGLLANFMVYLKKMYHMDQVSATSLMGLWTG 90
           + N+ K    GWK MP+I+GNETFE+L  +G L+N +VYL  ++++  ++A +++  ++G
Sbjct: 36  VQNQKKVVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATIINAFSG 95

Query: 91  VTSFLPLLGAFLSDAYIGRYWTIAIASIFSFLGMASMTLTAWLPQLHPPGCGGSVK--CL 150
             +F   + AFL D Y GRY T+++A I  FLG   + LTA +PQLHP  CG +    C 
Sbjct: 96  TINFGTFVAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAACGTAADSICN 155

Query: 151 TPTASQLGFLIMSLCLVSIGSGGIRPCSIPFGVDQFDPTTEKGRKGIASFYNWYYATFMV 210
            P+  Q+ FL+M L  + +G+GGIRPC++ FG DQF+P +E G++GI SF+NWY+ TF  
Sbjct: 156 GPSGGQIAFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKRGIDSFFNWYFFTFTF 215

Query: 211 VLVITLTIVVYIQDSVSWVLGYGIPTVLMFCSIILFFMGTHVYVFIKPEGSIFTGLAQVA 270
             +++LT+VVY+Q +VSW +G  IP VLMF + ++FF G  +YV IK  GS   G+AQV 
Sbjct: 216 AQILSLTLVVYVQSNVSWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLAGIAQVI 275

Query: 271 VAAYKKRHLKLPDDRKGFYDPPLDPASVVLSKLPLTNQFSFLNKAAIKT-ENDINPDGRR 330
             A KKR LK P  +         P     SKL  T+QF FL+KAAI T E+ + PDG+ 
Sbjct: 276 AVAIKKRGLK-PAKQPWLNLYNYYPPKYANSKLKYTDQFRFLDKAAILTPEDKLQPDGKP 335

Query: 331 VNKWNLSSIQQVEELKCLFRVFPIWVTGILSLTPIIQQSTFSISQALKMNRHMGS-NFQI 390
            + W L ++QQVEE+KC+ RV PIW    +    I QQ T+ + QAL+ +R +GS  F I
Sbjct: 336 ADPWKLCTMQQVEEVKCIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGSGGFVI 395

Query: 391 PPASIIVISFLTITFFIPFYDQFLVPTLRKFTGHPNGITELQRMAIGIVFAVLSMVVAGL 450
           P A+ +V     +T FI  YD+ LVPT+R+ TG   GIT LQR+  GI FA  S+VVAG 
Sbjct: 396 PAATYVVFLMTGMTVFIVVYDRVLVPTMRRITGLDTGITLLQRIGTGIFFATASLVVAGF 455

Query: 451 IEMQRR---------NQANNNGSI--MSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPE 510
           +E +RR           A   G I  MS  WL PQ  L G+ EAF  IGQ+EF+ K+FPE
Sbjct: 456 VEERRRTFALTKPTLGMAPRKGEISSMSAMWLIPQLSLAGVAEAFAAIGQMEFYYKQFPE 515

Query: 511 HMRTMGNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWLIDDLNKGRLDYFYYVV 570
           +MR+   +       +SSY+ + ++  VHRTT         +WL +DLNKGRLD FY+++
Sbjct: 516 NMRSFAGSIFYVGGGVSSYLGSFLIATVHRTTQNSSG---GNWLAEDLNKGRLDLFYFMI 575

Query: 571 AATAFFNFFFFLYCAKNYRYKGHVSDDE 584
           A     NF +FL  ++ YRYKG  SDDE
Sbjct: 576 AGILAVNFAYFLVMSRWYRYKG--SDDE 597

BLAST of CsaV3_5G032220.1 vs. ExPASy Swiss-Prot
Match: Q9M9V7 (Protein NRT1/ PTR FAMILY 2.9 OS=Arabidopsis thaliana OX=3702 GN=NPF2.9 PE=1 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 2.2e-127
Identity = 248/571 (43.43%), Postives = 360/571 (63.05%), Query Frame = 0

Query: 41  GWKSMPYILGNETFERLAAMGLLANFMVYLKKMYHMDQVSATSLMGLWTGVTSFLPLLGA 100
           GWK MP+I+GNETFE+L  +G  +N ++YL  +++M  ++A  ++ ++ G ++F  ++ A
Sbjct: 22  GWKVMPFIIGNETFEKLGIVGSSSNLVIYLTTVFNMKSITAAKVVNIYGGTSNFGTIVAA 81

Query: 101 FLSDAYIGRYWTIAIASIFSFLGMASMTLTAWLPQLHPPGCG---GSVKCLTPTASQLGF 160
           FL D+Y GRY T++ A I  FLG  +M LTA +  LHP  C    GSV C  P+  Q+ F
Sbjct: 82  FLCDSYFGRYKTLSFAMIACFLGSVAMDLTAVIHPLHPAQCAKEIGSV-CNGPSIGQIMF 141

Query: 161 LIMSLCLVSIGSGGIRPCSIPFGVDQFDPTTEKGRKGIASFYNWYYATFMVVLVITLTIV 220
           L  ++ L+ IG+GGIRPC++PFG DQFDP T++G++GI SF+NWY+ TF    +++LT++
Sbjct: 142 LAGAMVLLVIGAGGIRPCNLPFGADQFDPKTKEGKRGIESFFNWYFFTFTFAQMVSLTLI 201

Query: 221 VYIQDSVSWVLGYGIPTVLMFCSIILFFMGTHVYVFIKPEGSIFTGLAQVAVAAYKKRHL 280
           VY+Q +VSW +G  IP +LM    I+FF G+ +YV +K  GS    + +V V A KKR L
Sbjct: 202 VYVQSNVSWSIGLAIPAILMLLGCIIFFAGSKLYVKVKASGSPIHSITRVIVVAIKKRRL 261

Query: 281 KLPDDRKGFYDPPLDPASVVLSKLPLTNQFSFLNKAAIKTEND-INPDGRRVNKWNLSSI 340
           K P      Y+     +    SKL  T QF FL+K+AI+T++D +N DG  V+ W L S+
Sbjct: 262 K-PVGPNELYN--YIASDFKNSKLGHTEQFRFLDKSAIQTQDDKLNKDGSPVDAWKLCSM 321

Query: 341 QQVEELKCLFRVFPIWVTGILSLTPIIQQSTFSISQALKMNRHMG-SNFQIPPASIIVIS 400
           QQVEE+KC+ RV P+W++  L     IQQ+T++I Q+L+ +R +G  +FQIP  S  V  
Sbjct: 322 QQVEEVKCVIRVLPVWLSAALFYLAYIQQTTYTIFQSLQSDRRLGPGSFQIPAGSYTVFL 381

Query: 401 FLTITFFIPFYDQFLVPTLRKFTGHPNGITELQRMAIGIVFAVLSMVVAGLIEMQRR--- 460
            L +T FIP YD+ LVP LRK+TG   GIT+LQR+  G+   + SM+V+ ++E  RR   
Sbjct: 382 MLGMTIFIPIYDRVLVPFLRKYTGRDGGITQLQRVGAGLFLCITSMMVSAIVEQYRRKVA 441

Query: 461 ------NQANNNGSI--MSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPEHMRTMGNAF 520
                   A   G+I  MS  WL PQ  LMG+ +A   +GQ+EF+ K+FPE+MR+   + 
Sbjct: 442 LTKPTLGLAPRKGAISSMSGMWLIPQLVLMGIADALAGVGQMEFYYKQFPENMRSFAGSL 501

Query: 521 SSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWLIDDLNKGRLDYFYYVVAATAFFNFF 580
             C I L+SY++T ++  VH TT  G + G   WL +DLNKGRL+YFY++VA     N  
Sbjct: 502 YYCGIGLASYLSTFLLSAVHDTT-EGFSGG--SWLPEDLNKGRLEYFYFLVAGMMTLNLA 561

Query: 581 FFLYCAKNYRYKGHVSDDEQDLKLVISSDKV 596
           +FL  +  YRYK  V+ D+   K     DKV
Sbjct: 562 YFLLVSHWYRYKDVVAKDKDMDKTSAEFDKV 585

BLAST of CsaV3_5G032220.1 vs. ExPASy TrEMBL
Match: A0A0A0KTW3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G604170 PE=3 SV=1)

HSP 1 Score: 1191.0 bits (3080), Expect = 0.0e+00
Identity = 598/598 (100.00%), Postives = 598/598 (100.00%), Query Frame = 0

Query: 1   MESESERKASSNSRSPNNSKDANNNPKLSIISNKSKREPGGWKSMPYILGNETFERLAAM 60
           MESESERKASSNSRSPNNSKDANNNPKLSIISNKSKREPGGWKSMPYILGNETFERLAAM
Sbjct: 1   MESESERKASSNSRSPNNSKDANNNPKLSIISNKSKREPGGWKSMPYILGNETFERLAAM 60

Query: 61  GLLANFMVYLKKMYHMDQVSATSLMGLWTGVTSFLPLLGAFLSDAYIGRYWTIAIASIFS 120
           GLLANFMVYLKKMYHMDQVSATSLMGLWTGVTSFLPLLGAFLSDAYIGRYWTIAIASIFS
Sbjct: 61  GLLANFMVYLKKMYHMDQVSATSLMGLWTGVTSFLPLLGAFLSDAYIGRYWTIAIASIFS 120

Query: 121 FLGMASMTLTAWLPQLHPPGCGGSVKCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPFG 180
           FLGMASMTLTAWLPQLHPPGCGGSVKCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPFG
Sbjct: 121 FLGMASMTLTAWLPQLHPPGCGGSVKCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPFG 180

Query: 181 VDQFDPTTEKGRKGIASFYNWYYATFMVVLVITLTIVVYIQDSVSWVLGYGIPTVLMFCS 240
           VDQFDPTTEKGRKGIASFYNWYYATFMVVLVITLTIVVYIQDSVSWVLGYGIPTVLMFCS
Sbjct: 181 VDQFDPTTEKGRKGIASFYNWYYATFMVVLVITLTIVVYIQDSVSWVLGYGIPTVLMFCS 240

Query: 241 IILFFMGTHVYVFIKPEGSIFTGLAQVAVAAYKKRHLKLPDDRKGFYDPPLDPASVVLSK 300
           IILFFMGTHVYVFIKPEGSIFTGLAQVAVAAYKKRHLKLPDDRKGFYDPPLDPASVVLSK
Sbjct: 241 IILFFMGTHVYVFIKPEGSIFTGLAQVAVAAYKKRHLKLPDDRKGFYDPPLDPASVVLSK 300

Query: 301 LPLTNQFSFLNKAAIKTENDINPDGRRVNKWNLSSIQQVEELKCLFRVFPIWVTGILSLT 360
           LPLTNQFSFLNKAAIKTENDINPDGRRVNKWNLSSIQQVEELKCLFRVFPIWVTGILSLT
Sbjct: 301 LPLTNQFSFLNKAAIKTENDINPDGRRVNKWNLSSIQQVEELKCLFRVFPIWVTGILSLT 360

Query: 361 PIIQQSTFSISQALKMNRHMGSNFQIPPASIIVISFLTITFFIPFYDQFLVPTLRKFTGH 420
           PIIQQSTFSISQALKMNRHMGSNFQIPPASIIVISFLTITFFIPFYDQFLVPTLRKFTGH
Sbjct: 361 PIIQQSTFSISQALKMNRHMGSNFQIPPASIIVISFLTITFFIPFYDQFLVPTLRKFTGH 420

Query: 421 PNGITELQRMAIGIVFAVLSMVVAGLIEMQRRNQANNNGSIMSVFWLTPQFFLMGLCEAF 480
           PNGITELQRMAIGIVFAVLSMVVAGLIEMQRRNQANNNGSIMSVFWLTPQFFLMGLCEAF
Sbjct: 421 PNGITELQRMAIGIVFAVLSMVVAGLIEMQRRNQANNNGSIMSVFWLTPQFFLMGLCEAF 480

Query: 481 NIIGQIEFFNKEFPEHMRTMGNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWLI 540
           NIIGQIEFFNKEFPEHMRTMGNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWLI
Sbjct: 481 NIIGQIEFFNKEFPEHMRTMGNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWLI 540

Query: 541 DDLNKGRLDYFYYVVAATAFFNFFFFLYCAKNYRYKGHVSDDEQDLKLVISSDKVLDV 599
           DDLNKGRLDYFYYVVAATAFFNFFFFLYCAKNYRYKGHVSDDEQDLKLVISSDKVLDV
Sbjct: 541 DDLNKGRLDYFYYVVAATAFFNFFFFLYCAKNYRYKGHVSDDEQDLKLVISSDKVLDV 598

BLAST of CsaV3_5G032220.1 vs. ExPASy TrEMBL
Match: A0A1S3BEQ4 (LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 2.13-like OS=Cucumis melo OX=3656 GN=LOC103489074 PE=3 SV=1)

HSP 1 Score: 1131.3 bits (2925), Expect = 0.0e+00
Identity = 569/599 (94.99%), Postives = 583/599 (97.33%), Query Frame = 0

Query: 1   MESESERKASSNSRSPNNSKDA-NNNPKLSIISNKSKREPGGWKSMPYILGNETFERLAA 60
           MESESE+K SSNS SPNN KDA NNNPKLS ISNKSKREPGGWKSMPYILGNETFERLA+
Sbjct: 1   MESESEKKGSSNSHSPNNFKDANNNNPKLS-ISNKSKREPGGWKSMPYILGNETFERLAS 60

Query: 61  MGLLANFMVYLKKMYHMDQVSATSLMGLWTGVTSFLPLLGAFLSDAYIGRYWTIAIASIF 120
           MGLLANFMVYLK +YHMDQVSATSLMGLWTGVTSFLPLLGAFLSDAYIGRYWTIAIASIF
Sbjct: 61  MGLLANFMVYLKTVYHMDQVSATSLMGLWTGVTSFLPLLGAFLSDAYIGRYWTIAIASIF 120

Query: 121 SFLGMASMTLTAWLPQLHPPGCGGSVKCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPF 180
           SFLGMASMTLTAWLPQLHPPGCGGSVKCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPF
Sbjct: 121 SFLGMASMTLTAWLPQLHPPGCGGSVKCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPF 180

Query: 181 GVDQFDPTTEKGRKGIASFYNWYYATFMVVLVITLTIVVYIQDSVSWVLGYGIPTVLMFC 240
           GVDQFDPTTEKGRKGIASFYNWYYATFMVVLV TLTIVVYIQDSVSWVLGYGIPT LMFC
Sbjct: 181 GVDQFDPTTEKGRKGIASFYNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGLMFC 240

Query: 241 SIILFFMGTHVYVFIKPEGSIFTGLAQVAVAAYKKRHLKLPDDRKGFYDPPLDPASVVLS 300
           SIILFFMGTHVYVFIKPEGSIFTGLAQVAVAAYKKRHLKLPDDRKGFYDPPLDPASVVLS
Sbjct: 241 SIILFFMGTHVYVFIKPEGSIFTGLAQVAVAAYKKRHLKLPDDRKGFYDPPLDPASVVLS 300

Query: 301 KLPLTNQFSFLNKAAIKTENDINPDGRRVNKWNLSSIQQVEELKCLFRVFPIWVTGILSL 360
           KLPLTNQFSFLNKAAI TEN+I PDG R+NKWNLSSIQQVEELKCLFRVFPIWVTGILSL
Sbjct: 301 KLPLTNQFSFLNKAAIVTENEIKPDGTRLNKWNLSSIQQVEELKCLFRVFPIWVTGILSL 360

Query: 361 TPIIQQSTFSISQALKMNRHMGSNFQIPPASIIVISFLTITFFIPFYDQFLVPTLRKFTG 420
           TPIIQQSTFSISQAL+MNRHMGSNFQIPPASIIVISFLTITFFIP YD+FLVPTLRKFTG
Sbjct: 361 TPIIQQSTFSISQALQMNRHMGSNFQIPPASIIVISFLTITFFIPLYDRFLVPTLRKFTG 420

Query: 421 HPNGITELQRMAIGIVFAVLSMVVAGLIEMQRRNQANNNGSIMSVFWLTPQFFLMGLCEA 480
           HPNGITELQRMAIGI+FAVLSMVVAGL+EMQRRNQANN+GSIMSVFWLTPQFFLMGLCEA
Sbjct: 421 HPNGITELQRMAIGIIFAVLSMVVAGLVEMQRRNQANNDGSIMSVFWLTPQFFLMGLCEA 480

Query: 481 FNIIGQIEFFNKEFPEHMRTMGNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWL 540
           FNIIGQIEFFNKEFPEHMRTMGNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWL
Sbjct: 481 FNIIGQIEFFNKEFPEHMRTMGNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWL 540

Query: 541 IDDLNKGRLDYFYYVVAATAFFNFFFFLYCAKNYRYKGHVSDDEQDLKLVISSDKVLDV 599
           IDDLNKGRLDYFYY+VAATA F  F FLYCAKNYRYKGHVSDDEQDL+LV+SSDK+LDV
Sbjct: 541 IDDLNKGRLDYFYYIVAATAXFVIFLFLYCAKNYRYKGHVSDDEQDLQLVVSSDKLLDV 598

BLAST of CsaV3_5G032220.1 vs. ExPASy TrEMBL
Match: A0A5D3CUH6 (Protein NRT1/ PTR FAMILY 2.13-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00330 PE=3 SV=1)

HSP 1 Score: 1023.5 bits (2645), Expect = 3.6e-295
Identity = 505/523 (96.56%), Postives = 516/523 (98.66%), Query Frame = 0

Query: 76  MDQVSATSLMGLWTGVTSFLPLLGAFLSDAYIGRYWTIAIASIFSFLGMASMTLTAWLPQ 135
           MDQVSATSLMGLWTGVTSFLPLLGAFLSDAYIGRYWTIAIASIFSFLGMASMTLTAWLPQ
Sbjct: 1   MDQVSATSLMGLWTGVTSFLPLLGAFLSDAYIGRYWTIAIASIFSFLGMASMTLTAWLPQ 60

Query: 136 LHPPGCGGSVKCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPFGVDQFDPTTEKGRKGI 195
           LHPPGCGGSVKCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPFGVDQFDPTTEKGRKGI
Sbjct: 61  LHPPGCGGSVKCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPFGVDQFDPTTEKGRKGI 120

Query: 196 ASFYNWYYATFMVVLVITLTIVVYIQDSVSWVLGYGIPTVLMFCSIILFFMGTHVYVFIK 255
           ASFYNWYYATFMVVLV TLTIVVYIQDSVSWVLGYGIPT LMFCSIILFFMGTHVYVFIK
Sbjct: 121 ASFYNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGLMFCSIILFFMGTHVYVFIK 180

Query: 256 PEGSIFTGLAQVAVAAYKKRHLKLPDDRKGFYDPPLDPASVVLSKLPLTNQFSFLNKAAI 315
           PEGSIFTGLAQVAVAAYKKRHLKLPDDRKGFYDPPLDPASVVLSKLPLTNQFSFLNKAAI
Sbjct: 181 PEGSIFTGLAQVAVAAYKKRHLKLPDDRKGFYDPPLDPASVVLSKLPLTNQFSFLNKAAI 240

Query: 316 KTENDINPDGRRVNKWNLSSIQQVEELKCLFRVFPIWVTGILSLTPIIQQSTFSISQALK 375
            TEN+I PDG R+NKWNLSSIQQVEELKCLFRVFPIWVTGILSLTPIIQQSTFSISQAL+
Sbjct: 241 VTENEIKPDGTRLNKWNLSSIQQVEELKCLFRVFPIWVTGILSLTPIIQQSTFSISQALQ 300

Query: 376 MNRHMGSNFQIPPASIIVISFLTITFFIPFYDQFLVPTLRKFTGHPNGITELQRMAIGIV 435
           MNRHMGSNFQIPPASIIVISFLTITFFIP YD+FLVPTLRKFTGHPNGITELQRMAIGI+
Sbjct: 301 MNRHMGSNFQIPPASIIVISFLTITFFIPLYDRFLVPTLRKFTGHPNGITELQRMAIGII 360

Query: 436 FAVLSMVVAGLIEMQRRNQANNNGSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPE 495
           FAVLSMVVAGL+EMQRRNQANN+GSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPE
Sbjct: 361 FAVLSMVVAGLVEMQRRNQANNDGSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPE 420

Query: 496 HMRTMGNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWLIDDLNKGRLDYFYYVV 555
           HMRTMGNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWLIDDLNKGRLDYFYY+V
Sbjct: 421 HMRTMGNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWLIDDLNKGRLDYFYYIV 480

Query: 556 AATAFFNFFFFLYCAKNYRYKGHVSDDEQDLKLVISSDKVLDV 599
           AATAF NFFFFLYCAKNYRYKGHVSDDEQDL+LV+SSDK+LDV
Sbjct: 481 AATAFCNFFFFLYCAKNYRYKGHVSDDEQDLQLVVSSDKLLDV 523

BLAST of CsaV3_5G032220.1 vs. ExPASy TrEMBL
Match: A0A6J1KAA8 (protein NRT1/ PTR FAMILY 2.13-like OS=Cucurbita maxima OX=3661 GN=LOC111493070 PE=3 SV=1)

HSP 1 Score: 1015.8 bits (2625), Expect = 7.6e-293
Identity = 504/579 (87.05%), Postives = 535/579 (92.40%), Query Frame = 0

Query: 25  NPKLSII-SNKSKREPGGWKSMPYILGNETFERLAAMGLLANFMVYLKKMYHMDQVSATS 84
           NPKL     NK+KREPGGWKSMPYILGNETFERLA+MGLLANFMVYL K+YHMDQVSATS
Sbjct: 10  NPKLCFSPQNKAKREPGGWKSMPYILGNETFERLASMGLLANFMVYLTKVYHMDQVSATS 69

Query: 85  LMGLWTGVTSFLPLLGAFLSDAYIGRYWTIAIASIFSFLGMASMTLTAWLPQLHPPGCGG 144
           LMGLWTGVT+FLPLLGAFLSDAY GRYWTIA+AS FSFLGM SMTLTAWLPQLHPP C G
Sbjct: 70  LMGLWTGVTNFLPLLGAFLSDAYAGRYWTIAVASFFSFLGMTSMTLTAWLPQLHPPTCSG 129

Query: 145 SVKCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPFGVDQFDPTTEKGRKGIASFYNWYY 204
           +  C  PTASQLGFL+MSLCLVSIGS GIRPCSIPFGVDQFDPTT+KGRKGIASFYNWYY
Sbjct: 130 A-NCEGPTASQLGFLVMSLCLVSIGSAGIRPCSIPFGVDQFDPTTDKGRKGIASFYNWYY 189

Query: 205 ATFMVVLVITLTIVVYIQDSVSWVLGYGIPTVLMFCSIILFFMGTHVYVFIKPEGSIFTG 264
           ATF VVLV TLTIVVYIQDSVSWVLGYGIPTVLMFCSI+LFFMGTHVYVFIKPEGSIFTG
Sbjct: 190 ATFSVVLVFTLTIVVYIQDSVSWVLGYGIPTVLMFCSIVLFFMGTHVYVFIKPEGSIFTG 249

Query: 265 LAQVAVAAYKKRHLKLP--DDRKGFYDPPLDPASVVLSKLPLTNQFSFLNKAAIKTENDI 324
           LAQVAVAAYKKRHL+LP  DDR  FYDPPL+PASVVLSKLPLTNQFSFL KAAI  E ++
Sbjct: 250 LAQVAVAAYKKRHLELPSLDDRTAFYDPPLEPASVVLSKLPLTNQFSFLTKAAIVVEKEL 309

Query: 325 NPDGRRVNKWNLSSIQQVEELKCLFRVFPIWVTGILSLTPIIQQSTFSISQALKMNRHMG 384
           NPDG R+NKWNLSSIQQVEELKCL RV PIWVTGILSLTPIIQQ+TFSISQAL+M+RHMG
Sbjct: 310 NPDGTRINKWNLSSIQQVEELKCLVRVGPIWVTGILSLTPIIQQATFSISQALQMDRHMG 369

Query: 385 SNFQIPPASIIVISFLTITFFIPFYDQFLVPTLRKFTGHPNGITELQRMAIGIVFAVLSM 444
            NFQ+PPASIIVISFLTITFFIP YDQFLVP  RKFT HPNGITELQRMA+GI+FAVLSM
Sbjct: 370 PNFQMPPASIIVISFLTITFFIPLYDQFLVPAFRKFTQHPNGITELQRMAVGIIFAVLSM 429

Query: 445 VVAGLIEMQRRNQAN--NNGSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPEHMRT 504
           +VAG++E +RRN+AN  N GSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPEHMRT
Sbjct: 430 IVAGIVEKERRNRANGPNGGSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPEHMRT 489

Query: 505 MGNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWLIDDLNKGRLDYFYYVVAATA 564
           MGNAFSSCSIALSSY+NTAMVLIVHRTTG GGAHGKPDWL +DLNKGRLDYFYYVVAATA
Sbjct: 490 MGNAFSSCSIALSSYVNTAMVLIVHRTTGSGGAHGKPDWLTNDLNKGRLDYFYYVVAATA 549

Query: 565 FFNFFFFLYCAKNYRYKGHVSDDEQDLKLVISSDKVLDV 599
           FFNFFFF+YCAKNY YKG VSD +QDL+LVI+SDK LDV
Sbjct: 550 FFNFFFFIYCAKNYHYKGKVSDSQQDLELVIASDKKLDV 587

BLAST of CsaV3_5G032220.1 vs. ExPASy TrEMBL
Match: A0A6J1GZR8 (protein NRT1/ PTR FAMILY 2.13-like OS=Cucurbita moschata OX=3662 GN=LOC111458629 PE=3 SV=1)

HSP 1 Score: 1015.4 bits (2624), Expect = 9.9e-293
Identity = 503/578 (87.02%), Postives = 535/578 (92.56%), Query Frame = 0

Query: 25  NPKLSIISNKSKREPGGWKSMPYILGNETFERLAAMGLLANFMVYLKKMYHMDQVSATSL 84
           NPKL     KS+REPGGWKSMPYILGNETFERLA+MGLLANFMVYL+K+YHMDQVSATSL
Sbjct: 10  NPKL----RKSRREPGGWKSMPYILGNETFERLASMGLLANFMVYLRKVYHMDQVSATSL 69

Query: 85  MGLWTGVTSFLPLLGAFLSDAYIGRYWTIAIASIFSFLGMASMTLTAWLPQLHPPGCGGS 144
           MGLWTGVT+FLPLLGAFLSDAY GRYWTIA+AS FSFLGM SMTLTAWLPQLHPP C G+
Sbjct: 70  MGLWTGVTNFLPLLGAFLSDAYAGRYWTIAVASFFSFLGMTSMTLTAWLPQLHPPTCSGA 129

Query: 145 VKCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPFGVDQFDPTTEKGRKGIASFYNWYYA 204
             C  PTASQLGFL+MSLCLVSIGS GIRPCSIPFGVDQFDPTT+KGRKGIASFYNWYYA
Sbjct: 130 -NCEGPTASQLGFLVMSLCLVSIGSAGIRPCSIPFGVDQFDPTTDKGRKGIASFYNWYYA 189

Query: 205 TFMVVLVITLTIVVYIQDSVSWVLGYGIPTVLMFCSIILFFMGTHVYVFIKPEGSIFTGL 264
           TF VVLV TLTIVVYIQDSVSWVLGYGIPTVLMFCSI+LFFMGTHVYVFIKPEGSIFTGL
Sbjct: 190 TFSVVLVFTLTIVVYIQDSVSWVLGYGIPTVLMFCSIVLFFMGTHVYVFIKPEGSIFTGL 249

Query: 265 AQVAVAAYKKRHLKLP--DDRKGFYDPPLDPASVVLSKLPLTNQFSFLNKAAIKTENDIN 324
           AQVAVAAYKKRHL+LP  DDR  FYDPPL+P SVVLSKLPLTNQFSFLNKAAI  E ++N
Sbjct: 250 AQVAVAAYKKRHLELPSLDDRTAFYDPPLEPGSVVLSKLPLTNQFSFLNKAAIVVEKELN 309

Query: 325 PDGRRVNKWNLSSIQQVEELKCLFRVFPIWVTGILSLTPIIQQSTFSISQALKMNRHMGS 384
           PDG R+NKWNLSSIQQVEELKCL RV PIWVTGILSLTPIIQQ+TFSISQAL+M+RHMG 
Sbjct: 310 PDGTRINKWNLSSIQQVEELKCLVRVGPIWVTGILSLTPIIQQATFSISQALQMDRHMGP 369

Query: 385 NFQIPPASIIVISFLTITFFIPFYDQFLVPTLRKFTGHPNGITELQRMAIGIVFAVLSMV 444
           NFQ+PPASIIVISFLTITFFIP YDQFLVP  RKFT HPNGITELQRMA+GI+FAVLSM+
Sbjct: 370 NFQMPPASIIVISFLTITFFIPLYDQFLVPAFRKFTHHPNGITELQRMAVGIIFAVLSMI 429

Query: 445 VAGLIEMQRRNQAN--NNGSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPEHMRTM 504
           VAG++E +RRN+AN  N GSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPEHMRTM
Sbjct: 430 VAGIVEKERRNRANGPNGGSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPEHMRTM 489

Query: 505 GNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWLIDDLNKGRLDYFYYVVAATAF 564
           GNAFSSCSIALSSY+NTAMVLIVHRTTG GGAHGKPDWL +DLNKGRLDYFYYVVAATAF
Sbjct: 490 GNAFSSCSIALSSYVNTAMVLIVHRTTGSGGAHGKPDWLANDLNKGRLDYFYYVVAATAF 549

Query: 565 FNFFFFLYCAKNYRYKGHVSDDEQDLKLVISSDKVLDV 599
           FNFFFF+YCAKNY YKG VSD +QDL+LVI+SDK LDV
Sbjct: 550 FNFFFFIYCAKNYHYKGKVSDSQQDLELVIASDKKLDV 582

BLAST of CsaV3_5G032220.1 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 629.4 bits (1622), Expect = 2.9e-180
Identity = 309/574 (53.83%), Postives = 419/574 (73.00%), Query Frame = 0

Query: 24  NNPKLSIISNKSKREPGGWKSMPYILGNETFERLAAMGLLANFMVYLKKMYHMDQVSATS 83
           ++P   + + K +++PGGW+++ +ILGNET ERL ++GLLANFMVYL K++H++QV A +
Sbjct: 37  SSPGSILDAEKVEKKPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAAN 96

Query: 84  LMGLWTGVTSFLPLLGAFLSDAYIGRYWTIAIASIFSFLGMASMTLTAWLPQLHPPGCGG 143
           ++ +W+G T+  PL+GA++SD Y+GR+ TIA AS  + LG+ ++TLTA  PQLHP  C  
Sbjct: 97  VINIWSGFTNLTPLVGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNS 156

Query: 144 S--VKCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPFGVDQFDPTTEKGRKGIASFYNW 203
              + C  P   Q+G L++ LC +S+GSGGIRPCSIPFGVDQFD  TE+G KG+ASF+NW
Sbjct: 157 QDPLSCGGPNKLQIGVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNW 216

Query: 204 YYATFMVVLVITLTIVVYIQDSVSWVLGYGIPTVLMFCSIILFFMGTHVYVFIKPEGSIF 263
           YY TF VVL+IT T+VVYIQD VSW++G+ IPT LM  ++++FF G   YV++KPEGSIF
Sbjct: 217 YYMTFTVVLIITQTVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIF 276

Query: 264 TGLAQVAVAAYKKRHLKLPDDRKG---FYDPPLDPASVVLSKLPLTNQFSFLNKAAIKTE 323
           +G+AQV VAA KKR LKLP +  G   +YDP +   S VLSKL  +NQF  L+KAA+  E
Sbjct: 277 SGIAQVIVAARKKRKLKLPAEDDGTVTYYDPAI--KSSVLSKLHRSNQFRCLDKAAVVIE 336

Query: 324 NDINPDGRRVNKWNLSSIQQVEELKCLFRVFPIWVTGILSLTPIIQQSTFSISQALKMNR 383
            D+ P+G   +KW L S+Q+VEE+KCL R+ PIW  GI+SL  +  Q TF++SQALKM+R
Sbjct: 337 GDLTPEGPPADKWRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDR 396

Query: 384 HMGSNFQIPPASIIVISFLTITFFIPFYDQFLVPTLRKFTGHPNGITELQRMAIGIVFAV 443
           ++G  F+IP  S+ VIS LTI  F+PFYD+  VP +R+ TGH +GIT LQR+  GIVFA+
Sbjct: 397 NLGPKFEIPAGSLSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAI 456

Query: 444 LSMVVAGLIEMQRRNQANNNG-----SIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEF 503
            SM+VAG++E  RR ++ N G     + MSVFWL+PQ  LMGLCEAFNIIGQIEFFN +F
Sbjct: 457 FSMIVAGIVERMRRIRSINAGDPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQF 516

Query: 504 PEHMRTMGNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWLIDDLNKGRLDYFYY 563
           PEHMR++ N+  S S A SSY+++ +V +VH+ +   G H +PDWL  +LN G+LDYFYY
Sbjct: 517 PEHMRSIANSLFSLSFAGSSYLSSFLVTVVHKFS---GGHDRPDWLNKNLNAGKLDYFYY 576

Query: 564 VVAATAFFNFFFFLYCAKNYRYKG--HVSDDEQD 586
           ++A     N  +F YCA+ YRYK    + D E+D
Sbjct: 577 LIAVLGVVNLVYFWYCARGYRYKVGLPIEDFEED 605

BLAST of CsaV3_5G032220.1 vs. TAIR 10
Match: AT1G27080.1 (nitrate transporter 1.6 )

HSP 1 Score: 576.6 bits (1485), Expect = 2.3e-164
Identity = 292/560 (52.14%), Postives = 398/560 (71.07%), Query Frame = 0

Query: 28  LSIISNKS---KREPGGWKSMPYILGNETFERLAAMGLLANFMVYLKKMYHMDQVSATSL 87
           + ++ N+    +++ GGW+++ +ILGNET E+L ++G+ ANFM+YL+ ++HM+ V A ++
Sbjct: 1   MGVVENRKILPEKKLGGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNV 60

Query: 88  MGLWTGVTSFLPLLGAFLSDAYIGRYWTIAIASIFSFLGMASMTLTAWLPQLHPPGCGG- 147
             LW G+T+F PLLGA +SDAYIGR+ TIA AS+FS LG+ ++TLTA LPQLHPP C   
Sbjct: 61  YYLWMGLTNFAPLLGALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNP 120

Query: 148 -SVKCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPFGVDQFDPTTEKGRKGIASFYNWY 207
              +C  P   QLG L + L  +SIGSGGIRPCSIPFGVDQFD  TE+G KG+ASF+NWY
Sbjct: 121 HPDECDDPNKLQLGILFLGLGFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVASFFNWY 180

Query: 208 YATFMVVLVITLTIVVYIQDSVSWVLGYGIPTVLMFCSIILFFMGTHVYVFIKPEGSIFT 267
           Y T  +VL+ + T+VVY+Q +VSWV+G+ IPT LM C+++LFF+G   YV++KPEGS+F+
Sbjct: 181 YLTLTMVLIFSHTVVVYLQ-TVSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFS 240

Query: 268 GLAQVAVAAYKKRHLKL---PDDRKGFYDPPLDPASVVLSKLPLTNQFSFLNKAAIKTEN 327
           G+A+V VAA KKR LK+    D  + +Y+PP+ P   VLSKLPLT+QF FL+KAA+  + 
Sbjct: 241 GIARVIVAARKKRDLKISLVDDGTEEYYEPPVKPG--VLSKLPLTDQFKFLDKAAVILDG 300

Query: 328 DINPDGRRVNKWNLSSIQQVEELKCLFRVFPIWVTGILSLTPIIQQSTFSISQALKMNRH 387
           D+  +G   NKW L SIQ+VEE+KCL RV P+W  GI+S+  +  Q+TF + QA KM+RH
Sbjct: 301 DLTSEGVPANKWRLCSIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRH 360

Query: 388 MGSNFQIPPASIIVISFLTITFFIPFYDQFLVPTL---RKFTGHPNGITELQRMAIGIVF 447
           MG +F+IP ASI VIS++TI  ++P Y+  LVP L   RKF      +T LQRM IGIVF
Sbjct: 361 MGPHFEIPAASITVISYITIGIWVPIYEHLLVPFLWRMRKFR-----VTLLQRMGIGIVF 420

Query: 448 AVLSMVVAGLIEMQRRNQANNNGSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPEH 507
           A+LSM  AG +E  RR +A    + MSVFWL     LMGLCE+FN IG IEFFN +FPEH
Sbjct: 421 AILSMFTAGFVEGVRRTRATEM-TQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEH 480

Query: 508 MRTMGNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWLIDDLNKGRLDYFYYVVA 567
           MR++ N+    S A ++Y+++ +V  VH+ +   G    PDWL  DL++G+LDYFYY++A
Sbjct: 481 MRSIANSLFPLSFAAANYLSSLLVTTVHKVS---GTKDHPDWLNKDLDRGKLDYFYYLIA 540

Query: 568 ATAFFNFFFFLYCAKNYRYK 577
                N  +F YCA  Y+YK
Sbjct: 541 VLGVVNLVYFWYCAHRYQYK 548

BLAST of CsaV3_5G032220.1 vs. TAIR 10
Match: AT1G69860.1 (Major facilitator superfamily protein )

HSP 1 Score: 506.1 bits (1302), Expect = 3.8e-143
Identity = 251/550 (45.64%), Postives = 377/550 (68.55%), Query Frame = 0

Query: 36  KREPGGWKSMPYILGNETFERLAAMGLLANFMVYLKKMYHMDQVSATSLMGLWTGVTSFL 95
           +R+P GWK+MPYI+GNET ERLA  GL+ANFMVY+ + YHMDQV A +L+  W+ +T+F 
Sbjct: 16  QRKPLGWKAMPYIIGNETLERLATFGLMANFMVYMVREYHMDQVQAVTLINTWSALTNFA 75

Query: 96  PLLGAFLSDAYIGRYWTIAIASIFSFLGMASMTLTAWLPQLHPPGCGG---SVKCLTPTA 155
           P++GAF+SD+Y G++ TI   SI   LGM  +T T+ +P L PP C     + +C+  + 
Sbjct: 76  PIIGAFISDSYTGKFNTIVFGSIAELLGMLVLTFTSLVPNLRPPPCTADQITGQCIPYSY 135

Query: 156 SQLGFLIMSLCLVSIGSGGIRPCSIPFGVDQFDPTTEKGRKGIASFYNWYYATFMVVLVI 215
           SQL  L+  L L+S+G+GGIR CS+PF +DQFD +TE+GR+G  SF++WYY T  +V ++
Sbjct: 136 SQLYVLLSGLFLLSVGTGGIRSCSVPFSLDQFDDSTEEGREGSRSFFSWYYTTHTIVQLV 195

Query: 216 TLTIVVYIQDSVSWVLGYGIPTVLMFCSIILFFMGTHVYVFIKPEGSIFTGLAQVAVAAY 275
           ++T+V+Y+Q+++SW +G+ IPTVL F +++L F+GT  YVF+KPEGS+F+G+ +V VAAY
Sbjct: 196 SMTLVLYVQNNISWGIGFAIPTVLNFFALLLLFVGTRYYVFVKPEGSVFSGVFKVLVAAY 255

Query: 276 KKRHLKLPDDRKGFYDPPLDPASVVLSKLPLTNQFSFLNKAAIKTENDINPDGRRVNKWN 335
           KKR  +        Y  PL    +  +KL LT+QF FLNKA I   ND   +     +W 
Sbjct: 256 KKRKARFTSGID--YHQPLLETDLQSNKLVLTDQFRFLNKAVIVMNNDEAGN----EEWR 315

Query: 336 LSSIQQVEELKCLFRVFPIWVTGILSLTPIIQQSTFSISQALKMN-RHMGSNFQIPPASI 395
             +++Q+E++K +  + PI+ + I+    + QQ TF++SQALKM+ +  G+++ IPPASI
Sbjct: 316 TCTVRQIEDIKSIISIIPIFASSIIGFLAMNQQQTFTVSQALKMDLQFPGTSYLIPPASI 375

Query: 396 IVISFLTITFFIPFYDQFLVPTLRKFTGHPNGITELQRMAIGIVFAVLSMVVAGLIEMQR 455
            VIS L I  ++PFY+  LV  +   T    GI+ LQ++ IG +F++ +M+++G++E +R
Sbjct: 376 TVISLLNIGIWLPFYETVLVRHIENITKQNGGISLLQKVGIGNIFSISTMLISGIVERKR 435

Query: 456 RNQANNNGSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPEHMRTMGNAFSSCSIAL 515
           R+  + NG  MSVFWLTPQ  LMG  + F I+G  EFFNK+ P +MR++GN+     ++L
Sbjct: 436 RD-LSLNGVKMSVFWLTPQQVLMGFYQVFTIVGLTEFFNKQVPINMRSIGNSLLYLGLSL 495

Query: 516 SSYINTAMVLIVHRTTGRGGAHGKPDWLIDDLNKGRLDYFYYVVAATAFFNFFFFLYCAK 575
           +SY+++AMV IVH  T RGG      WL DD++K +LD FYY +AA +  NF FF +CA+
Sbjct: 496 ASYLSSAMVSIVHSVTARGG----QSWLTDDIDKSKLDCFYYFIAALSTLNFIFFFWCAR 554

Query: 576 NYRYKGHVSD 582
            YRY+ + ++
Sbjct: 556 RYRYRNYSNE 554

BLAST of CsaV3_5G032220.1 vs. TAIR 10
Match: AT5G62680.1 (Major facilitator superfamily protein )

HSP 1 Score: 463.4 bits (1191), Expect = 2.8e-130
Identity = 251/568 (44.19%), Postives = 351/568 (61.80%), Query Frame = 0

Query: 31  ISNKSKREPGGWKSMPYILGNETFERLAAMGLLANFMVYLKKMYHMDQVSATSLMGLWTG 90
           + N+ K    GWK MP+I+GNETFE+L  +G L+N +VYL  ++++  ++A +++  ++G
Sbjct: 36  VQNQKKVVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATIINAFSG 95

Query: 91  VTSFLPLLGAFLSDAYIGRYWTIAIASIFSFLGMASMTLTAWLPQLHPPGCGGSVK--CL 150
             +F   + AFL D Y GRY T+++A I  FLG   + LTA +PQLHP  CG +    C 
Sbjct: 96  TINFGTFVAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAACGTAADSICN 155

Query: 151 TPTASQLGFLIMSLCLVSIGSGGIRPCSIPFGVDQFDPTTEKGRKGIASFYNWYYATFMV 210
            P+  Q+ FL+M L  + +G+GGIRPC++ FG DQF+P +E G++GI SF+NWY+ TF  
Sbjct: 156 GPSGGQIAFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKRGIDSFFNWYFFTFTF 215

Query: 211 VLVITLTIVVYIQDSVSWVLGYGIPTVLMFCSIILFFMGTHVYVFIKPEGSIFTGLAQVA 270
             +++LT+VVY+Q +VSW +G  IP VLMF + ++FF G  +YV IK  GS   G+AQV 
Sbjct: 216 AQILSLTLVVYVQSNVSWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLAGIAQVI 275

Query: 271 VAAYKKRHLKLPDDRKGFYDPPLDPASVVLSKLPLTNQFSFLNKAAIKT-ENDINPDGRR 330
             A KKR LK P  +         P     SKL  T+QF FL+KAAI T E+ + PDG+ 
Sbjct: 276 AVAIKKRGLK-PAKQPWLNLYNYYPPKYANSKLKYTDQFRFLDKAAILTPEDKLQPDGKP 335

Query: 331 VNKWNLSSIQQVEELKCLFRVFPIWVTGILSLTPIIQQSTFSISQALKMNRHMGS-NFQI 390
            + W L ++QQVEE+KC+ RV PIW    +    I QQ T+ + QAL+ +R +GS  F I
Sbjct: 336 ADPWKLCTMQQVEEVKCIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGSGGFVI 395

Query: 391 PPASIIVISFLTITFFIPFYDQFLVPTLRKFTGHPNGITELQRMAIGIVFAVLSMVVAGL 450
           P A+ +V     +T FI  YD+ LVPT+R+ TG   GIT LQR+  GI FA  S+VVAG 
Sbjct: 396 PAATYVVFLMTGMTVFIVVYDRVLVPTMRRITGLDTGITLLQRIGTGIFFATASLVVAGF 455

Query: 451 IEMQRR---------NQANNNGSI--MSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPE 510
           +E +RR           A   G I  MS  WL PQ  L G+ EAF  IGQ+EF+ K+FPE
Sbjct: 456 VEERRRTFALTKPTLGMAPRKGEISSMSAMWLIPQLSLAGVAEAFAAIGQMEFYYKQFPE 515

Query: 511 HMRTMGNAFSSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWLIDDLNKGRLDYFYYVV 570
           +MR+   +       +SSY+ + ++  VHRTT         +WL +DLNKGRLD FY+++
Sbjct: 516 NMRSFAGSIFYVGGGVSSYLGSFLIATVHRTTQNSSG---GNWLAEDLNKGRLDLFYFMI 575

Query: 571 AATAFFNFFFFLYCAKNYRYKGHVSDDE 584
           A     NF +FL  ++ YRYKG  SDDE
Sbjct: 576 AGILAVNFAYFLVMSRWYRYKG--SDDE 597

BLAST of CsaV3_5G032220.1 vs. TAIR 10
Match: AT1G18880.1 (Major facilitator superfamily protein )

HSP 1 Score: 457.6 bits (1176), Expect = 1.5e-128
Identity = 248/571 (43.43%), Postives = 360/571 (63.05%), Query Frame = 0

Query: 41  GWKSMPYILGNETFERLAAMGLLANFMVYLKKMYHMDQVSATSLMGLWTGVTSFLPLLGA 100
           GWK MP+I+GNETFE+L  +G  +N ++YL  +++M  ++A  ++ ++ G ++F  ++ A
Sbjct: 22  GWKVMPFIIGNETFEKLGIVGSSSNLVIYLTTVFNMKSITAAKVVNIYGGTSNFGTIVAA 81

Query: 101 FLSDAYIGRYWTIAIASIFSFLGMASMTLTAWLPQLHPPGCG---GSVKCLTPTASQLGF 160
           FL D+Y GRY T++ A I  FLG  +M LTA +  LHP  C    GSV C  P+  Q+ F
Sbjct: 82  FLCDSYFGRYKTLSFAMIACFLGSVAMDLTAVIHPLHPAQCAKEIGSV-CNGPSIGQIMF 141

Query: 161 LIMSLCLVSIGSGGIRPCSIPFGVDQFDPTTEKGRKGIASFYNWYYATFMVVLVITLTIV 220
           L  ++ L+ IG+GGIRPC++PFG DQFDP T++G++GI SF+NWY+ TF    +++LT++
Sbjct: 142 LAGAMVLLVIGAGGIRPCNLPFGADQFDPKTKEGKRGIESFFNWYFFTFTFAQMVSLTLI 201

Query: 221 VYIQDSVSWVLGYGIPTVLMFCSIILFFMGTHVYVFIKPEGSIFTGLAQVAVAAYKKRHL 280
           VY+Q +VSW +G  IP +LM    I+FF G+ +YV +K  GS    + +V V A KKR L
Sbjct: 202 VYVQSNVSWSIGLAIPAILMLLGCIIFFAGSKLYVKVKASGSPIHSITRVIVVAIKKRRL 261

Query: 281 KLPDDRKGFYDPPLDPASVVLSKLPLTNQFSFLNKAAIKTEND-INPDGRRVNKWNLSSI 340
           K P      Y+     +    SKL  T QF FL+K+AI+T++D +N DG  V+ W L S+
Sbjct: 262 K-PVGPNELYN--YIASDFKNSKLGHTEQFRFLDKSAIQTQDDKLNKDGSPVDAWKLCSM 321

Query: 341 QQVEELKCLFRVFPIWVTGILSLTPIIQQSTFSISQALKMNRHMG-SNFQIPPASIIVIS 400
           QQVEE+KC+ RV P+W++  L     IQQ+T++I Q+L+ +R +G  +FQIP  S  V  
Sbjct: 322 QQVEEVKCVIRVLPVWLSAALFYLAYIQQTTYTIFQSLQSDRRLGPGSFQIPAGSYTVFL 381

Query: 401 FLTITFFIPFYDQFLVPTLRKFTGHPNGITELQRMAIGIVFAVLSMVVAGLIEMQRR--- 460
            L +T FIP YD+ LVP LRK+TG   GIT+LQR+  G+   + SM+V+ ++E  RR   
Sbjct: 382 MLGMTIFIPIYDRVLVPFLRKYTGRDGGITQLQRVGAGLFLCITSMMVSAIVEQYRRKVA 441

Query: 461 ------NQANNNGSI--MSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPEHMRTMGNAF 520
                   A   G+I  MS  WL PQ  LMG+ +A   +GQ+EF+ K+FPE+MR+   + 
Sbjct: 442 LTKPTLGLAPRKGAISSMSGMWLIPQLVLMGIADALAGVGQMEFYYKQFPENMRSFAGSL 501

Query: 521 SSCSIALSSYINTAMVLIVHRTTGRGGAHGKPDWLIDDLNKGRLDYFYYVVAATAFFNFF 580
             C I L+SY++T ++  VH TT  G + G   WL +DLNKGRL+YFY++VA     N  
Sbjct: 502 YYCGIGLASYLSTFLLSAVHDTT-EGFSGG--SWLPEDLNKGRLEYFYFLVAGMMTLNLA 561

Query: 581 FFLYCAKNYRYKGHVSDDEQDLKLVISSDKV 596
           +FL  +  YRYK  V+ D+   K     DKV
Sbjct: 562 YFLLVSHWYRYKDVVAKDKDMDKTSAEFDKV 585

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004135202.10.0e+00100.00protein NRT1/ PTR FAMILY 2.13 [Cucumis sativus] >KGN51872.1 hypothetical protein... [more]
XP_008446297.20.0e+0094.99PREDICTED: LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 2.13-like [Cucumis melo... [more]
XP_038892122.13.2e-30990.48protein NRT1/ PTR FAMILY 2.13-like [Benincasa hispida][more]
KAA0034378.17.5e-29596.56protein NRT1/ PTR FAMILY 2.13-like [Cucumis melo var. makuwa] >TYK15541.1 protei... [more]
XP_022998476.11.6e-29287.05protein NRT1/ PTR FAMILY 2.13-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8RX774.1e-17953.83Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV... [more]
Q9LFX93.2e-16352.14Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana OX=3702 GN=NPF2.12 PE=1 SV... [more]
Q9CAR95.3e-14245.64Putative protein NRT1/ PTR FAMILY 2.14 OS=Arabidopsis thaliana OX=3702 GN=NPF2.1... [more]
Q9LV103.9e-12944.19Protein NRT1/ PTR FAMILY 2.11 OS=Arabidopsis thaliana OX=3702 GN=NPF2.11 PE=1 SV... [more]
Q9M9V72.2e-12743.43Protein NRT1/ PTR FAMILY 2.9 OS=Arabidopsis thaliana OX=3702 GN=NPF2.9 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KTW30.0e+00100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G604170 PE=3 SV=1[more]
A0A1S3BEQ40.0e+0094.99LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 2.13-like OS=Cucumis melo OX=3656 ... [more]
A0A5D3CUH63.6e-29596.56Protein NRT1/ PTR FAMILY 2.13-like OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A6J1KAA87.6e-29387.05protein NRT1/ PTR FAMILY 2.13-like OS=Cucurbita maxima OX=3661 GN=LOC111493070 P... [more]
A0A6J1GZR89.9e-29387.02protein NRT1/ PTR FAMILY 2.13-like OS=Cucurbita moschata OX=3662 GN=LOC111458629... [more]
Match NameE-valueIdentityDescription
AT1G69870.12.9e-18053.83nitrate transporter 1.7 [more]
AT1G27080.12.3e-16452.14nitrate transporter 1.6 [more]
AT1G69860.13.8e-14345.64Major facilitator superfamily protein [more]
AT5G62680.12.8e-13044.19Major facilitator superfamily protein [more]
AT1G18880.11.5e-12843.43Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 110..529
e-value: 7.5E-83
score: 278.6
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 27..584
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 16..582
e-value: 1.1E-172
score: 577.0
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 59..574
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 10..32
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..37
NoneNo IPR availablePANTHERPTHR11654:SF378PEPTIDE/NITRATE TRANSPORTERcoord: 27..584
NoneNo IPR availableCDDcd17416MFS_NPF1_2coord: 45..573
e-value: 0.0
score: 562.273
IPR018456PTR2 family proton/oligopeptide symporter, conserved sitePROSITEPS01022PTR2_1coord: 99..123

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CsaV3_5G032220CsaV3_5G032220gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CsaV3_5G032220.1.exon4CsaV3_5G032220.1.exon4exon
CsaV3_5G032220.1.exon3CsaV3_5G032220.1.exon3exon
CsaV3_5G032220.1.exon2CsaV3_5G032220.1.exon2exon
CsaV3_5G032220.1.exon1CsaV3_5G032220.1.exon1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CsaV3_5G032220.1.cds4CsaV3_5G032220.1.cds4CDS
CsaV3_5G032220.1.cds3CsaV3_5G032220.1.cds3CDS
CsaV3_5G032220.1.cds2CsaV3_5G032220.1.cds2CDS
CsaV3_5G032220.1.cds1CsaV3_5G032220.1.cds1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CsaV3_5G032220.1CsaV3_5G032220.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006857 oligopeptide transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity