CsGy7G007250.1 (mRNA) Cucumber (Gy14) v2.1

Overview


Name	CsGy7G007250.1
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	calmodulin-like protein 1
Location	Gy14Chr7: 5224483 .. 5225364 (+)
Sequence length	882
RNA-Seq Expression	CsGy7G007250.1
Synteny	CsGy7G007250.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAATCAAACTCTCTTTTTTCTCTCTCTCTCACTATTTAAACTCTTCAAATCCATCTTCCTTCATCTTCAATTTCATTGCCTATCTTCCACTGTTTCAATAAACAAAAAACAGAGCAACCTTGTTTTCACTACTCAACCACTGAAATTCCATCTGGGTTCCTCTAAATTTTGAAAACCAATCTTATTTTCATCCAAAAACACAAAAACAGTGCAACCCATCGTTAAAAAAAATGCCAATTAACCCGAATTTCCTTGAACTACAGTACAACGTCTCAAAGAAAAAGTTCCTCCGTGAACCTTCCCGGCTGTTCTCCTTCAAGGACCGGCAGAGCTCTGGCCTCCGGCGGGCACTGCAGCCGAGCTCGGAGGAGATAAAGCAGATCTTCAACAAGTTTGACACGAACAAAGACGGGAGGATCTCGAAGCATGAATACAGGGGAATTCTGAAGGCGTTGGGGAGAGGGAATTCGATGGAAGAAGTTCAGAAGATTTTTAGGGCGGTGGATTCCGACGGCGATGGGTATATAAATTTGAATGAGTTTATGGAGGTTCACCGGAGCGGCGGGGGAGTTCAGGCAAAAGAGGTGGAATTTGCCTTCAAGACGTTTGATTTGAATGGGGATCGGAAGATTAGTGCAGAGGAAGTGATGAGAGTGTTGAAGGGATTGGGGGAGAAGTGTAGCATTGAGGATTGCCGGCGAATGGTGAGGGCGGTGGATAGCGACGGCGACGGCATGGTGGACATTAATGAGTTTATGACCATGATGACGCGCTCTGCTAAGTGAAAGCTTTCTTCTTCTTCTTCTTCTTCTTTCAATGCAAAGTAAAAGTGTTTGTGTAATATAAAGGCTTTGTGTTCATTGGAATTATAGCTTTCCATTC

mRNA sequence

GAATCAAACTCTCTTTTTTCTCTCTCTCTCACTATTTAAACTCTTCAAATCCATCTTCCTTCATCTTCAATTTCATTGCCTATCTTCCACTGTTTCAATAAACAAAAAACAGAGCAACCTTGTTTTCACTACTCAACCACTGAAATTCCATCTGGGTTCCTCTAAATTTTGAAAACCAATCTTATTTTCATCCAAAAACACAAAAACAGTGCAACCCATCGTTAAAAAAAATGCCAATTAACCCGAATTTCCTTGAACTACAGTACAACGTCTCAAAGAAAAAGTTCCTCCGTGAACCTTCCCGGCTGTTCTCCTTCAAGGACCGGCAGAGCTCTGGCCTCCGGCGGGCACTGCAGCCGAGCTCGGAGGAGATAAAGCAGATCTTCAACAAGTTTGACACGAACAAAGACGGGAGGATCTCGAAGCATGAATACAGGGGAATTCTGAAGGCGTTGGGGAGAGGGAATTCGATGGAAGAAGTTCAGAAGATTTTTAGGGCGGTGGATTCCGACGGCGATGGGTATATAAATTTGAATGAGTTTATGGAGGTTCACCGGAGCGGCGGGGGAGTTCAGGCAAAAGAGGTGGAATTTGCCTTCAAGACGTTTGATTTGAATGGGGATCGGAAGATTAGTGCAGAGGAAGTGATGAGAGTGTTGAAGGGATTGGGGGAGAAGTGTAGCATTGAGGATTGCCGGCGAATGGTGAGGGCGGTGGATAGCGACGGCGACGGCATGGTGGACATTAATGAGTTTATGACCATGATGACGCGCTCTGCTAAGTGAAAGCTTTCTTCTTCTTCTTCTTCTTCTTTCAATGCAAAGTAAAAGTGTTTGTGTAATATAAAGGCTTTGTGTTCATTGGAATTATAGCTTTCCATTC

Coding sequence (CDS)

ATGCCAATTAACCCGAATTTCCTTGAACTACAGTACAACGTCTCAAAGAAAAAGTTCCTCCGTGAACCTTCCCGGCTGTTCTCCTTCAAGGACCGGCAGAGCTCTGGCCTCCGGCGGGCACTGCAGCCGAGCTCGGAGGAGATAAAGCAGATCTTCAACAAGTTTGACACGAACAAAGACGGGAGGATCTCGAAGCATGAATACAGGGGAATTCTGAAGGCGTTGGGGAGAGGGAATTCGATGGAAGAAGTTCAGAAGATTTTTAGGGCGGTGGATTCCGACGGCGATGGGTATATAAATTTGAATGAGTTTATGGAGGTTCACCGGAGCGGCGGGGGAGTTCAGGCAAAAGAGGTGGAATTTGCCTTCAAGACGTTTGATTTGAATGGGGATCGGAAGATTAGTGCAGAGGAAGTGATGAGAGTGTTGAAGGGATTGGGGGAGAAGTGTAGCATTGAGGATTGCCGGCGAATGGTGAGGGCGGTGGATAGCGACGGCGACGGCATGGTGGACATTAATGAGTTTATGACCATGATGACGCGCTCTGCTAAGTGA

Protein sequence

MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVEFAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMTRSAK*

Homology

[Close]

BLAST of CsGy7G007250.1 vs. ExPASy Swiss-Prot
Match: Q9ZQE6 (Calmodulin-like protein 30 OS=Arabidopsis thaliana OX=3702 GN=CML30 PE=2 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 7.8e-52
Identity = 101/182 (55.49%), Postives = 143/182 (78.57%), Query Frame = 0


Query: 4   NPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRA-----LQPSSEEIKQIFNKFDTN 63
           N +FLELQY +SK K LR+PSR+FS +DRQSSGL         QPS  E++++F++FD +
Sbjct: 3   NVSFLELQYKLSKNKMLRKPSRMFS-RDRQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLD 62

Query: 64  KDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKE 123
           KDG+IS+ EY+ +L+ALG+  ++E+V KIF+AVD DGDG+I+  EF++ ++  GG+++ +
Sbjct: 63  KDGKISQTEYKVVLRALGQERAIEDVPKIFKAVDLDGDGFIDFREFIDAYKRSGGIRSSD 122

Query: 124 VEFAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTM 181
           +  +F TFDLNGD KISAEEVM VL  LGE+CS+EDC RMVRAVD+DGDG+V++ EF+ M
Sbjct: 123 IRNSFWTFDLNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIKM 182

[Close]

BLAST of CsGy7G007250.1 vs. ExPASy Swiss-Prot
Match: Q9LE22 (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 PE=1 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 2.3e-27
Identity = 63/138 (45.65%), Postives = 90/138 (65.22%), Query Frame = 0


Query: 46  EEIKQIFNKFDTNKDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFM 105
           EE+K++F++FD+N DG+IS  E  G+ KA+G   +  E+ ++   VD+D DGYINL+EF 
Sbjct: 22  EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 106 EVHRSGGGVQAKEVEFAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSD 165
            + RS     A E+  AF  +D + +  ISA E+ +VL  LG  CS+EDC RM+  VD+D
Sbjct: 82  TLCRSSS--SAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDAD 141

Query: 166 GDGMVDINEFMTMMTRSA 184
           GDG V+  EF  MMT S+
Sbjct: 142 GDGNVNFEEFQKMMTSSS 157

[Close]

BLAST of CsGy7G007250.1 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 3.0e-27
Identity = 63/150 (42.00%), Postives = 95/150 (63.33%), Query Frame = 0


Query: 34  SSGLRRALQPSSEEIKQIFNKFDTNKDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDS 93
           S  + R    S E+IK++F +FD N DG+IS  E + ++ AL    S EE + + +  D 
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 61

Query: 94  DGDGYINLNEFMEV----HRSGGGVQAKEVEFAFKTFDLNGDRKISAEEVMRVLKGLGEK 153
           DG+G+I+L+EF+ +     +S      ++++ AF  +DL+ + +ISA E+  V+K LGEK
Sbjct: 62  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 121

Query: 154 CSIEDCRRMVRAVDSDGDGMVDINEFMTMM 180
           CSI+DC+RM+  VDSDGDG VD  EF  MM
Sbjct: 122 CSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151

[Close]

BLAST of CsGy7G007250.1 vs. ExPASy Swiss-Prot
Match: Q9FYK2 (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 3.0e-27
Identity = 59/140 (42.14%), Postives = 88/140 (62.86%), Query Frame = 0


Query: 47  EIKQIFNKFDTNKDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFME 106
           E++ +F KFD N DG+IS  E   I+ +LG     EE++K    +D  GDGYIN  EF+E
Sbjct: 37  ELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVE 96

Query: 107 VHRSGGGVQ--AKEVEFAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDS 166
           ++  G       + ++ AF  +D++G+  ISAEE+  VL+ LG++CSI +CR+M+  VD 
Sbjct: 97  LNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDK 156

Query: 167 DGDGMVDINEFMTMMTRSAK 185
           DGDG +D  EF  MMT  ++
Sbjct: 157 DGDGTIDFEEFKIMMTMGSR 176

[Close]

BLAST of CsGy7G007250.1 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 5.1e-27
Identity = 62/152 (40.79%), Postives = 96/152 (63.16%), Query Frame = 0


Query: 31  DRQSSGLRRALQPSSEEIKQIFNKFDTNKDGRISKHEYRGILKALGRGNSMEEVQKIFRA 90
           D  S G   A  P  EE++++F KFD N DGRIS+ E   + ++LG   + +E+ ++   
Sbjct: 25  DPASGGGSPAPTP-EEEMERVFRKFDANGDGRISRSELGALFESLGHAATDDELARMMAE 84

Query: 91  VDSDGDGYINLNEFMEVHRSGGGVQA---KEVEFAFKTFDLNGDRKISAEEVMRVLKGLG 150
            D+DGDG+I+L+EF  ++ +  G  A   +++  AF+ FD +G+  ISA E+ RVL GLG
Sbjct: 85  ADADGDGFISLDEFAALNATASGDAAAVEEDLRHAFRVFDADGNGTISAAELARVLHGLG 144

Query: 151 EKCSIEDCRRMVRAVDSDGDGMVDINEFMTMM 180
           EK +++ CRRM+  VD +GDG++   EF  MM
Sbjct: 145 EKATVQQCRRMIEGVDQNGDGLISFEEFKVMM 175

[Close]

BLAST of CsGy7G007250.1 vs. NCBI nr
Match: XP_004137114.2 (calmodulin-like protein 30 [Cucumis sativus] >KGN43936.1 hypothetical protein Csa_017094 [Cucumis sativus])

HSP 1 Score: 365 bits (936), Expect = 4.24e-127
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 0


Query: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60
           MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD
Sbjct: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60

Query: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120
           GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE
Sbjct: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120

Query: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180
           FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

Query: 181 RSAK 184
           RSAK
Sbjct: 181 RSAK 184

[Close]

BLAST of CsGy7G007250.1 vs. NCBI nr
Match: XP_008454944.1 (PREDICTED: calmodulin-like protein 1 [Cucumis melo] >KAA0031292.1 calmodulin-like protein 1 [Cucumis melo var. makuwa] >TYK06744.1 calmodulin-like protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 359 bits (922), Expect = 5.79e-125
Identity = 181/184 (98.37%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60
           MPINPNFL+LQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPS EEIKQIFNKFDTNKD
Sbjct: 1   MPINPNFLDLQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSLEEIKQIFNKFDTNKD 60

Query: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120
           GRISKHEYRGILKALGRG+SMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE
Sbjct: 61  GRISKHEYRGILKALGRGSSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120

Query: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180
           FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

Query: 181 RSAK 184
           RSAK
Sbjct: 181 RSAK 184

[Close]

BLAST of CsGy7G007250.1 vs. NCBI nr
Match: XP_038888181.1 (calmodulin-like protein 30 [Benincasa hispida])

HSP 1 Score: 344 bits (882), Expect = 7.53e-119
Identity = 171/184 (92.93%), Postives = 180/184 (97.83%), Query Frame = 0


Query: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60
           MPINPN L+LQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPS+EEIKQIFNKFD N+D
Sbjct: 1   MPINPNLLDLQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSTEEIKQIFNKFDRNRD 60

Query: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120
           GRISK+EYRGILKALGR NSMEEVQ IFRAVD+DGDGYINLNEFMEVHRSGGGVQA+E+E
Sbjct: 61  GRISKNEYRGILKALGRENSMEEVQNIFRAVDADGDGYINLNEFMEVHRSGGGVQAREIE 120

Query: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180
           FAFKTFDLNGDRKISAEEVMRVL+GLGEKCSIEDCRRMVRAVD+DGDGMVDINEFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLRGLGEKCSIEDCRRMVRAVDNDGDGMVDINEFMTMMT 180

Query: 181 RSAK 184
           RSAK
Sbjct: 181 RSAK 184

[Close]

BLAST of CsGy7G007250.1 vs. NCBI nr
Match: XP_022988967.1 (calmodulin-like protein 1 [Cucurbita maxima])

HSP 1 Score: 342 bits (877), Expect = 4.20e-118
Identity = 168/183 (91.80%), Postives = 179/183 (97.81%), Query Frame = 0


Query: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60
           MP+NPN L+LQY VSKKKFLREPSRLFSFKDRQSSG+RRALQPS+EEIKQIFNKFDTN+D
Sbjct: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60

Query: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120
           GRISKHEYRG+LKALGRGNSMEEVQKIFRAVD+DGDGYINLNEFMEVHRSG GVQA+EVE
Sbjct: 61  GRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQAREVE 120

Query: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180
           FAFKTFDLNGDRKISAEE+MRVL+GLGEKCSIEDCRRMVRAVDSDGDGMVDI+EFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180

Query: 181 RSA 183
           RS 
Sbjct: 181 RSV 183

[Close]

BLAST of CsGy7G007250.1 vs. NCBI nr
Match: KAG6588656.1 (Calmodulin-like protein 30, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013282.1 Calmodulin-like protein 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 341 bits (874), Expect = 1.21e-117
Identity = 167/183 (91.26%), Postives = 179/183 (97.81%), Query Frame = 0


Query: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60
           MP+NPN L+LQY VSKKKFLREPSRLFSFKDRQSSG+RRALQPS+EEIKQIFNKFDTN+D
Sbjct: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60

Query: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120
           GRISKHEYRG+LKALGRGNSMEEVQKIFRAVD+DGDGYINLNEFMEVHRSG GVQA++VE
Sbjct: 61  GRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVE 120

Query: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180
           FAFKTFDLNGDRKISAEE+MRVL+GLGEKCSIEDCRRMVRAVDSDGDGMVDI+EFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180

Query: 181 RSA 183
           RS 
Sbjct: 181 RSV 183

[Close]

BLAST of CsGy7G007250.1 vs. ExPASy TrEMBL
Match: A0A0A0K804 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G073720 PE=4 SV=1)

HSP 1 Score: 365 bits (936), Expect = 2.05e-127
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 0


Query: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60
           MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD
Sbjct: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60

Query: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120
           GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE
Sbjct: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120

Query: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180
           FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

Query: 181 RSAK 184
           RSAK
Sbjct: 181 RSAK 184

[Close]

BLAST of CsGy7G007250.1 vs. ExPASy TrEMBL
Match: A0A5D3C643 (Calmodulin-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00450 PE=4 SV=1)

HSP 1 Score: 359 bits (922), Expect = 2.80e-125
Identity = 181/184 (98.37%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60
           MPINPNFL+LQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPS EEIKQIFNKFDTNKD
Sbjct: 1   MPINPNFLDLQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSLEEIKQIFNKFDTNKD 60

Query: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120
           GRISKHEYRGILKALGRG+SMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE
Sbjct: 61  GRISKHEYRGILKALGRGSSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120

Query: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180
           FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

Query: 181 RSAK 184
           RSAK
Sbjct: 181 RSAK 184

[Close]

BLAST of CsGy7G007250.1 vs. ExPASy TrEMBL
Match: A0A1S3C0X9 (calmodulin-like protein 1 OS=Cucumis melo OX=3656 GN=LOC103495233 PE=4 SV=1)

HSP 1 Score: 359 bits (922), Expect = 2.80e-125
Identity = 181/184 (98.37%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60
           MPINPNFL+LQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPS EEIKQIFNKFDTNKD
Sbjct: 1   MPINPNFLDLQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSLEEIKQIFNKFDTNKD 60

Query: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120
           GRISKHEYRGILKALGRG+SMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE
Sbjct: 61  GRISKHEYRGILKALGRGSSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120

Query: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180
           FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

Query: 181 RSAK 184
           RSAK
Sbjct: 181 RSAK 184

[Close]

BLAST of CsGy7G007250.1 vs. ExPASy TrEMBL
Match: A0A6J1JEH1 (calmodulin-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111486169 PE=4 SV=1)

HSP 1 Score: 342 bits (877), Expect = 2.04e-118
Identity = 168/183 (91.80%), Postives = 179/183 (97.81%), Query Frame = 0


Query: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60
           MP+NPN L+LQY VSKKKFLREPSRLFSFKDRQSSG+RRALQPS+EEIKQIFNKFDTN+D
Sbjct: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60

Query: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120
           GRISKHEYRG+LKALGRGNSMEEVQKIFRAVD+DGDGYINLNEFMEVHRSG GVQA+EVE
Sbjct: 61  GRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQAREVE 120

Query: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180
           FAFKTFDLNGDRKISAEE+MRVL+GLGEKCSIEDCRRMVRAVDSDGDGMVDI+EFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180

Query: 181 RSA 183
           RS 
Sbjct: 181 RSV 183

[Close]

BLAST of CsGy7G007250.1 vs. ExPASy TrEMBL
Match: A0A6J1ELL0 (calmodulin-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111434472 PE=4 SV=1)

HSP 1 Score: 338 bits (867), Expect = 6.81e-117
Identity = 166/183 (90.71%), Postives = 178/183 (97.27%), Query Frame = 0


Query: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60
           MP+NPN L+LQY VSKKKFLREPSRLFSFKDRQSSG+RRALQPS+EEIKQIFNKFDTN+D
Sbjct: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60

Query: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120
           G ISKHEYRG+LKALGRGNSMEEVQKIFRAVD+DGDGYINLNEFMEVHRSG GVQA++VE
Sbjct: 61  GLISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVE 120

Query: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180
           FAFKTFDLNGDRKISAEE+MRVL+GLGEKCSIEDCRRMVRAVDSDGDGMVDI+EFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180

Query: 181 RSA 183
           RS 
Sbjct: 181 RSV 183

[Close]

BLAST of CsGy7G007250.1 vs. TAIR 10
Match: AT2G15680.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 204.9 bits (520), Expect = 5.5e-53
Identity = 101/182 (55.49%), Postives = 143/182 (78.57%), Query Frame = 0


Query: 4   NPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRA-----LQPSSEEIKQIFNKFDTN 63
           N +FLELQY +SK K LR+PSR+FS +DRQSSGL         QPS  E++++F++FD +
Sbjct: 3   NVSFLELQYKLSKNKMLRKPSRMFS-RDRQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLD 62

Query: 64  KDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKE 123
           KDG+IS+ EY+ +L+ALG+  ++E+V KIF+AVD DGDG+I+  EF++ ++  GG+++ +
Sbjct: 63  KDGKISQTEYKVVLRALGQERAIEDVPKIFKAVDLDGDGFIDFREFIDAYKRSGGIRSSD 122

Query: 124 VEFAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTM 181
           +  +F TFDLNGD KISAEEVM VL  LGE+CS+EDC RMVRAVD+DGDG+V++ EF+ M
Sbjct: 123 IRNSFWTFDLNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIKM 182

[Close]

BLAST of CsGy7G007250.1 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 123.6 bits (309), Expect = 1.6e-28
Identity = 63/138 (45.65%), Postives = 90/138 (65.22%), Query Frame = 0


Query: 46  EEIKQIFNKFDTNKDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFM 105
           EE+K++F++FD+N DG+IS  E  G+ KA+G   +  E+ ++   VD+D DGYINL+EF 
Sbjct: 22  EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 106 EVHRSGGGVQAKEVEFAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSD 165
            + RS     A E+  AF  +D + +  ISA E+ +VL  LG  CS+EDC RM+  VD+D
Sbjct: 82  TLCRSSS--SAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDAD 141

Query: 166 GDGMVDINEFMTMMTRSA 184
           GDG V+  EF  MMT S+
Sbjct: 142 GDGNVNFEEFQKMMTSSS 157

[Close]

BLAST of CsGy7G007250.1 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 123.6 bits (309), Expect = 1.6e-28
Identity = 63/138 (45.65%), Postives = 90/138 (65.22%), Query Frame = 0


Query: 46  EEIKQIFNKFDTNKDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFM 105
           EE+K++F++FD+N DG+IS  E  G+ KA+G   +  E+ ++   VD+D DGYINL+EF 
Sbjct: 22  EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 106 EVHRSGGGVQAKEVEFAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSD 165
            + RS     A E+  AF  +D + +  ISA E+ +VL  LG  CS+EDC RM+  VD+D
Sbjct: 82  TLCRSSS--SAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDAD 141

Query: 166 GDGMVDINEFMTMMTRSA 184
           GDG V+  EF  MMT S+
Sbjct: 142 GDGNVNFEEFQKMMTSSS 157

[Close]

BLAST of CsGy7G007250.1 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 123.2 bits (308), Expect = 2.1e-28
Identity = 59/140 (42.14%), Postives = 88/140 (62.86%), Query Frame = 0


Query: 47  EIKQIFNKFDTNKDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFME 106
           E++ +F KFD N DG+IS  E   I+ +LG     EE++K    +D  GDGYIN  EF+E
Sbjct: 37  ELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVE 96

Query: 107 VHRSGGGVQ--AKEVEFAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDS 166
           ++  G       + ++ AF  +D++G+  ISAEE+  VL+ LG++CSI +CR+M+  VD 
Sbjct: 97  LNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDK 156

Query: 167 DGDGMVDINEFMTMMTRSAK 185
           DGDG +D  EF  MMT  ++
Sbjct: 157 DGDGTIDFEEFKIMMTMGSR 176

[Close]

BLAST of CsGy7G007250.1 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 123.2 bits (308), Expect = 2.1e-28
Identity = 63/150 (42.00%), Postives = 95/150 (63.33%), Query Frame = 0


Query: 34  SSGLRRALQPSSEEIKQIFNKFDTNKDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDS 93
           S  + R    S E+IK++F +FD N DG+IS  E + ++ AL    S EE + + +  D 
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 61

Query: 94  DGDGYINLNEFMEV----HRSGGGVQAKEVEFAFKTFDLNGDRKISAEEVMRVLKGLGEK 153
           DG+G+I+L+EF+ +     +S      ++++ AF  +DL+ + +ISA E+  V+K LGEK
Sbjct: 62  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 121

Query: 154 CSIEDCRRMVRAVDSDGDGMVDINEFMTMM 180
           CSI+DC+RM+  VDSDGDG VD  EF  MM
Sbjct: 122 CSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151

The following BLAST results are available for this feature:

BLAST of CsGy7G007250.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZQE6	7.8e-52	55.49	Calmodulin-like protein 30 OS=Arabidopsis thaliana OX=3702 GN=CML30 PE=2 SV=1	[more]
Q9LE22	2.3e-27	45.65	Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 ...	[more]
Q9C8Y1	3.0e-27	42.00	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ...	[more]
Q9FYK2	3.0e-27	42.14	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ...	[more]
Q8RZB5	5.1e-27	40.79	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of CsGy7G007250.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004137114.2	4.24e-127	100.00	calmodulin-like protein 30 [Cucumis sativus] >KGN43936.1 hypothetical protein Cs...	[more]
XP_008454944.1	5.79e-125	98.37	PREDICTED: calmodulin-like protein 1 [Cucumis melo] >KAA0031292.1 calmodulin-lik...	[more]
XP_038888181.1	7.53e-119	92.93	calmodulin-like protein 30 [Benincasa hispida]	[more]
XP_022988967.1	4.20e-118	91.80	calmodulin-like protein 1 [Cucurbita maxima]	[more]
KAG6588656.1	1.21e-117	91.26	Calmodulin-like protein 30, partial [Cucurbita argyrosperma subsp. sororia] >KAG...	[more]

BLAST of CsGy7G007250.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K804	2.05e-127	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G073720 PE=4 SV=1	[more]
A0A5D3C643	2.80e-125	98.37	Calmodulin-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3C0X9	2.80e-125	98.37	calmodulin-like protein 1 OS=Cucumis melo OX=3656 GN=LOC103495233 PE=4 SV=1	[more]
A0A6J1JEH1	2.04e-118	91.80	calmodulin-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111486169 PE=4 SV=1	[more]
A0A6J1ELL0	6.81e-117	90.71	calmodulin-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111434472 PE=4 SV=...	[more]

BLAST of CsGy7G007250.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G15680.1	5.5e-53	55.49	Calcium-binding EF-hand family protein	[more]
AT1G18210.1	1.6e-28	45.65	Calcium-binding EF-hand family protein	[more]
AT1G18210.2	1.6e-28	45.65	Calcium-binding EF-hand family protein	[more]
AT1G24620.1	2.1e-28	42.14	EF hand calcium-binding protein family	[more]
AT1G66400.1	2.1e-28	42.00	calmodulin like 23	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 118..146 e-value: 3.6E-4 score: 29.9 coord: 47..75 e-value: 6.0E-7 score: 39.1 coord: 154..182 e-value: 0.0086 score: 25.3 coord: 83..111 e-value: 8.3E-5 score: 32.0
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 119..179 e-value: 1.2E-11 score: 44.8 coord: 46..106 e-value: 7.1E-15 score: 55.2
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 150..184 score: 11.417016
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 79..114 score: 14.42972
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 117..149 score: 12.811786
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 43..78 score: 15.936071
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 118..180 e-value: 6.38272E-18 score: 71.8101
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 47..109 e-value: 5.65219E-19 score: 74.5065
None	No IPR available	GENE3D	1.10.238.10		coord: 114..184 e-value: 1.0E-20 score: 75.5
None	No IPR available	GENE3D	1.10.238.10		coord: 6..111 e-value: 7.3E-24 score: 86.7
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 6..183
None	No IPR available	PANTHER	PTHR10891:SF685	CALCIUM-BINDING PROTEIN CML34-RELATED	coord: 6..183
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 92..104
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 56..68
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 127..139
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 163..175
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 46..180

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CsGy7G007250	CsGy7G007250	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CsGy7G007250.1.utr5p1	CsGy7G007250.1.utr5p1	five_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CsGy7G007250.1.exon1	CsGy7G007250.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.CsGy7G007250.1	cds.CsGy7G007250.1	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CsGy7G007250.1.utr3p1	CsGy7G007250.1.utr3p1	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CsGy7G007250.1	CsGy7G007250.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0005509	calcium ion binding