CsGy2G026490.2 (mRNA) Cucumber (Gy14) v2.1

Overview


Name	CsGy2G026490.2
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like
Location	Gy14Chr2: 34111120 .. 34116187 (+)
Sequence length	2255
RNA-Seq Expression	CsGy2G026490.2
Synteny	CsGy2G026490.2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTCAGTAGGTCGGTGATTGATTTGACTTTTCCATCCAGTTCAAATCCAGGATAAGGATTTGGGAGAGATATATGGTGTCCTGTGATTGGATCCTTCTCTTTGTCCACAGAAGTCATCACTTCTACATGAAAAACTTCACGAGAGGGGCCTATAAATAGATGAAGCTTGACCATTTTGAAGTTGCTTCTACCCCGAGTTGAATTAAGAAGGAATTGAGGAGATCTCCAAAGTTTTCAAAATTCTCATAAACTTGAATATCGGCAAAGAAGTTGCAAGGAAGGGAGTTCTAGACGGGGGAACTGGACGAAGAAGCTAGCTGTTTGATTAAGTGTGAGTGGTTCTTCTTACAAGATGTTTTCAAAATGAAAATGATTTCTAAAATGTATTTGAATTTGTATATGATTTCATTTATGCTTTCCAATTCTTAAAAATGCATATGATTTATAAATGAATTAGACTTATTATGACGGGAATGATGTGACAAAATAATATGTATTTGAGAATGACATGAGATTTAAAATGAATGGAATGATGAAACAAGAAAGATCCTTTGTATCCTATGTGATGAGAATACCTCATATGTATGTGATATTTTATCCTTGTTGTGTGATATGAGGTAAGATGCTCTATTTTGGTTGTGTTCTAGAATAAAATGTCTTATTCATCTTGAGTAGGATGCATCATATATACTTGGTGGATGATCTGATATGAGATGTCATTTGCTTGTTGTGTGATCTGGTATCGAAAGACGTGTTATGTGTGATGAAATTGGTGAAATTAGTTAAATGCATTAAGATTACTGCACATAGTTGTAACTTGGCAAAATAGTGTTGTAAACAATTGAACTGAGAAAAAAAGGTGTAGAGATGGTTATGTTGTATGCAAAATTTGTATTACGAAGTTATATGAAATGGTACAAACAAATTTTAAATTTAAAATCACAAACTGTAGGGACCAAAATTTGAAGTTTAATGAAAGTTAAAGATTATTACATATTTGAAGGAAGAGGTTATAGATTTTATAAAGTGATGTATTTTCAATTACATACAAAATGTTATTTTAAAAAATTATAATGGGTGTTAATCATAGATTTAAAAACAAAATTTCATAAATAGAATTGGGATAGTCTCATGTGATAACATAAAACAAAATTGATGATTTAAAACTGAGTTTGGCAAGAAACAAAATGGTTGGAGGGAAATATATTTACGTGAAAAACCTAATCCATAAGGCATAAACCCCGAAAGAAATAGATTCCATTTGCACCCTAAAATCTCCTCTACCGGACCGGCGGCGGCGATGGGCTGCCTAACCATCCTCATCCTTAGGTTATGAGATCTCTTTCCAATTCTCATCTTCATCTCAAATATATATTCTCTCTCCCTAACTCTCTCATGGCTTCCTCTCTTATACAAACCCTTAAACCAGATTTAGCTCCTCAAGACCTAGACGACCTTCTCTGTTCCCCTTCACACAAACAGCCACAACACGAATCCTCCTCTCTTACCAATTCCACTTCCAATGACTCAACTCCTCTCTTGGGGTCGCCGACTGGATATTTGAGTCGCGAGTCGCGCATTGAACGAGCCTGGGCTCACTGGACCAAGCTCGGTCGCCCAAAGTTGATTGTAGCTCCCATGGTTGATAATTCGGAGTTGCCATTTCGTTTACTCTGCAGGAAATATGGTGCTGAGGCTGCTTATACGCCTATGCTCCATTCACGAATCTTTACCGAGAACGAGAAGTATCGGAACATGGAATTCACCACTTGTCAGGTATGTGACAATTTAAAGCCATTTTATTGTGTTAACAACTTAAATATTCTGTTTCATTTGGAATTCATGTATGTTTCCGGCTGCTTTTTGTGGATCGGTAGCTAATTGTAGGTTTTGGTCTTGGCTGTACTATTGTTTATGCTTATTTTTCATTGTTTTTCAATTTTTGAATGCTTTGTAGGAGGACCGTCCATTATTTGTTCAATTTTGTGCCAATGATCCCGATGTTCTGTTGGAGGCTGCTAGAAGAGTGGAGCCATATTGTGATTACGTCGATCTTAACCTTGGGTACTCTCTATTACTCTAATTTGTACTTGGTTGATTAATTCGTATGGATGTTTGCATTCTTTATGGTCGTATGATCCAAATGCTGACTTCTTATAATTCTTGTCGGTGGTGGAGTGAGGATTTGTTCTCCAAAATACTTTATGTTTGTTAAGATTGTGGATTGTTCACCAAGTACATTTTGTCATTAAGAAGTAACAAAACAAAACTACAAAAGAAGGGAGCCCTCAGTAGGAGGATCTACAAAATACTTCTCCAATTAGAGAGCAGGAAAGGAAAGCTATAGTAATGAAAAAGAGGGGAGCATTTACGCCAAGTCACACAAACTAACTATTGTCCCAAAAATATCCTTAAAATTTTTTCTATTACGCTATTGGCAAATATCCTTAAAGAGCACTCACATAACGTTCCCGCAAGAAGCCTCGTTTCCTTTCTGAATGGATGACCCCTTGAACTTCAACTGTTTAAGCAATAAAGATCTTTGGATTCTCTGGTAGGACCATATGCTAAAAAACACCTTCATAATAGCCAGGAGATTCAAATACTTCATTCCCTAAGGAGGACACTATGTCTTGGACCATTTTAAACTTTATTATAAGTTGATGCATGTGCATGTAAAGGTATCCGAGAGAACTTGATATTGAGGAGTTTGAAAATCCTTGAGGATGTCATTTTGTCCCAGACAAAGGTATTTGGAAAATGGGGAAAATGGCGTCTTTTCTGAATGGTTCAGCTTTTCAAATAAGAAAAACTGAAAATATTCAGCCAGTGGTGAGCTTAGTCAAATTTGAACCAAATATACTCGAATCCGATCAAAGATTGAATAGTTTGCTAAGTAATGGAAGTGGTGCACCCGGTTAAAATTTTGCTTCCATATCCTAGAGGGCTTGAGTTCGATCCTCCGTCCTTGAGATTGGATCTTTTTAAAGACATTGGCGGCTTTCCTTCTGTTCAGCCAAATTTTTTCAGCACATCCCATTTGCAGTTCACAGGTTCTAAGCCCAACCCAACGCTGTGTGCCATTAACCAAATGGTGTACTGCTTCTAAACACTAAACCAACAGTTTGGTAGGTTTGGTTATTATGTGTGACAATTCAGTTCGTTTTTTAAATTTCTTCCTAGATTTAGCAATTTACTTTTTGATACTCTAAACTAACAAAAACAATCTAGGATAATCTGAGTTCACTTGCTTTCCAAATGTGTTGCATACCAAATATGTTGATTGTGTTCATATTTTTCACTTAAGGTTCCTGAACGTCAATTGAGCAGTCAAGTAATTTTGTGCAGGTGTCCTCAGCGTATTGCAAGGCGAGGAAATTATGGGGCTTTTTTAATGGATAACCTACCTCTTGTAAAAGCAATAGTAGAGAAACTGGCTTCCAATCTTCAAGTTCCTGTATCATGCAAAATCCGAATCTTCCCAAATCTACAGGATACAATTAATTATGCAAGAATGTTAGAGGATGCTGGATGCTCTCTTTTAGCTGTTCATGGCCGCACAAGAGATGAAAAAGATGGTAAAAAATTCCGAGCTAACTGGAGTGCCATTAGTGCGGTTAAAAACGCTGTACGAATTCCAGTTCTTGCCAATGGGAATATACGGCACATGGAAGATGTTAACGACTGTTTACAAGAGACGGGAGTTGAGGGTGTGCTCTCGGCAGAAACCCTTCTGGAGAACCCTGCACTTTTTGCTGGGTTTCGAACTGCTGAATGGATAGAGGGCCACGAAGAAAATACCCGGGATGGTAACCTGGATCAAGCGGATCTGCTTGTAGACTACTTGAAGCTTTGTGAAAAATACCCTGTCCCATGGAGAATGATCCGTTCGCATGTACACAAGTTATTGGGAGATTGGTTCAAGATTCATCCACACATTAGAGAAGACTTCAATGCACAATCGAAGCTCACCTTTGAATTTCTTTACAATATGGTGGAGCGGCTGAGAGAATTGCAGGTAAAAATGCCTCTTTATATCAAAGATTCTCATGCACTTGCAATTTCATCCAATGCTTGATTGATTCCACACATAATAATTGATAGGTTTATGGTCTTAACTTAAAGATTCTTTGAGGAAATTAGATTGATACTGTTGTTGTACCACTAATATTCACATAAAATTGTCTTTCTTCAATCAGCTTGAGCACACAGTGAAGAAAGAAAAGATTTTGTATTTATTCTTGCGAATTGTAGCCTTATTTGATTTAAATAGGTAAGAGAGATAAGAGAGTAAACATTACCTTTTGGCTTTGCCCACATTATCTATTCTACCATCTTGTTTACATTCTTCTAGAAACATTTTTCATTGTGTTCAAATGTTCATAGTGATTTGATGCGAGACTCTTGAAAATGTAATTCTTCGACATGTTTCATTTTAGTTCAACAAATGTTCGGGGTGGAGATTAAACTATTGACACCTTTGAAATAGTAATTGATATTTTATTTATGAAGCTATGCTTGGATTGATACTTATATTTCACATTGATCTTTGTATGATCGAACACTACTGGGTCAAACAATCTTCATCCTAATTGAATAAGAAAAGTAAAGATCGTACGCTATGCAACTTGATTCTTATGAACAGTTTATTTATCGCATGTTTGGAGTTTCTTTTGGACACTTTGGAGTGAGCACAATAGTCGACTTCTCAGAGACAGTCTGTTCTTTCGATAGATTTTTGGATTTTGTTTCTTGCACATGTTTTCAATTCCTGTGATTTCTGATCTTCCTTTTTGTTCCTCTATCTTATCCTTTTTATTTTCATTATCATAAGAGAGATTTCTTGTACTAATATATTCTCTCTTTTCGTTTCTATTTTTTTTTTTTAAAAAAAGATCACGGTTGGCCCATCTCTTCAAAGTTGTAGTTAAGTTGGCTCTGAACAGATTATGCTTGAACTTTGTAAGAGCAAGTATGTGCACATACGTGATCAAACTTTTCATTAATGTTCAATCATGTTCTTTCAAAATAGTTTTTTAACTAGCAATTATTTAGGATTGGAATGGGTA

mRNA sequence

GTCAGTAGGTCGGTGATTGATTTGACTTTTCCATCCAGTTCAAATCCAGGATAAGGATTTGGGAGAGATATATGGTGTCCTGTGATTGGATCCTTCTCTTTGTCCACAGAAGTCATCACTTCTACATGAAAAACTTCACGAGAGGGGCCTATAAATAGATGAAGCTTGACCATTTTGAAGTTGCTTCTACCCCGAGTTGAATTAAGAAGGAATTGAGGAGATCTCCAAAGTTTTCAAAATTCTCATAAACTTGAATATCGGCAAAGAAGTTGCAAGGAAGGGAGTTCTAGACGGGGGAACTGGACGAAGAAGCTAGCTGTTTGATTAAGTGAAATATGGTGCTGAGGCTGCTTATACGCCTATGCTCCATTCACGAATCTTTACCGAGAACGAGAAGTATCGGAACATGGAATTCACCACTTGTCAGGAGGACCGTCCATTATTTGTTCAATTTTGTGCCAATGATCCCGATGTTCTGTTGGAGGCTGCTAGAAGAGTGGAGCCATATTGTGATTACGTCGATCTTAACCTTGGGTGTCCTCAGCGTATTGCAAGGCGAGGAAATTATGGGGCTTTTTTAATGGATAACCTACCTCTTGTAAAAGCAATAGTAGAGAAACTGGCTTCCAATCTTCAAGTTCCTGTATCATGCAAAATCCGAATCTTCCCAAATCTACAGGATACAATTAATTATGCAAGAATGTTAGAGGATGCTGGATGCTCTCTTTTAGCTGTTCATGGCCGCACAAGAGATGAAAAAGATGGTAAAAAATTCCGAGCTAACTGGAGTGCCATTAGTGCGGTTAAAAACGCTGTACGAATTCCAGTTCTTGCCAATGGGAATATACGGCACATGGAAGATGTTAACGACTGTTTACAAGAGACGGGAGTTGAGGGTGTGCTCTCGGCAGAAACCCTTCTGGAGAACCCTGCACTTTTTGCTGGGTTTCGAACTGCTGAATGGATAGAGGGCCACGAAGAAAATACCCGGGATGGTAACCTGGATCAAGCGGATCTGCTTGTAGACTACTTGAAGCTTTGTGAAAAATACCCTGTCCCATGGAGAATGATCCGTTCGCATGTACACAAGTTATTGGGAGATTGGTTCAAGATTCATCCACACATTAGAGAAGACTTCAATGCACAATCGAAGCTCACCTTTGAATTTCTTTACAATATGGTGGAGCGGCTGAGAGAATTGCAGGTAAAAATGCCTCTTTATATCAAAGATTCTCATGCACTTGCAATTTCATCCAATGCTTGATTGATTCCACACATAATAATTGATAGGTTTATGGTCTTAACTTAAAGATTCTTTGAGGAAATTAGATTGATACTGTTGTTGTACCACTAATATTCACATAAAATTGTCTTTCTTCAATCAGCTTGAGCACACAGTGAAGAAAGAAAAGATTTTGTATTTATTCTTGCGAATTGTAGCCTTATTTGATTTAAATAGGTAAGAGAGATAAGAGAGTAAACATTACCTTTTGGCTTTGCCCACATTATCTATTCTACCATCTTGTTTACATTCTTCTAGAAACATTTTTCATTGTGTTCAAATGTTCATAGTGATTTGATGCGAGACTCTTGAAAATGTAATTCTTCGACATGTTTCATTTTAGTTCAACAAATGTTCGGGGTGGAGATTAAACTATTGACACCTTTGAAATAGTAATTGATATTTTATTTATGAAGCTATGCTTGGATTGATACTTATATTTCACATTGATCTTTGTATGATCGAACACTACTGGGTCAAACAATCTTCATCCTAATTGAATAAGAAAAGTAAAGATCGTACGCTATGCAACTTGATTCTTATGAACAGTTTATTTATCGCATGTTTGGAGTTTCTTTTGGACACTTTGGAGTGAGCACAATAGTCGACTTCTCAGAGACAGTCTGTTCTTTCGATAGATTTTTGGATTTTGTTTCTTGCACATGTTTTCAATTCCTGTGATTTCTGATCTTCCTTTTTGTTCCTCTATCTTATCCTTTTTATTTTCATTATCATAAGAGAGATTTCTTGTACTAATATATTCTCTCTTTTCGTTTCTATTTTTTTTTTTTAAAAAAAGATCACGGTTGGCCCATCTCTTCAAAGTTGTAGTTAAGTTGGCTCTGAACAGATTATGCTTGAACTTTGTAAGAGCAAGTATGTGCACATACGTGATCAAACTTTTCATTAATGTTCAATCATGTTCTTTCAAAATAGTTTTTTAACTAGCAATTATTTAGGATTGGAATGGGTA

Coding sequence (CDS)

ATGCTCCATTCACGAATCTTTACCGAGAACGAGAAGTATCGGAACATGGAATTCACCACTTGTCAGGAGGACCGTCCATTATTTGTTCAATTTTGTGCCAATGATCCCGATGTTCTGTTGGAGGCTGCTAGAAGAGTGGAGCCATATTGTGATTACGTCGATCTTAACCTTGGGTGTCCTCAGCGTATTGCAAGGCGAGGAAATTATGGGGCTTTTTTAATGGATAACCTACCTCTTGTAAAAGCAATAGTAGAGAAACTGGCTTCCAATCTTCAAGTTCCTGTATCATGCAAAATCCGAATCTTCCCAAATCTACAGGATACAATTAATTATGCAAGAATGTTAGAGGATGCTGGATGCTCTCTTTTAGCTGTTCATGGCCGCACAAGAGATGAAAAAGATGGTAAAAAATTCCGAGCTAACTGGAGTGCCATTAGTGCGGTTAAAAACGCTGTACGAATTCCAGTTCTTGCCAATGGGAATATACGGCACATGGAAGATGTTAACGACTGTTTACAAGAGACGGGAGTTGAGGGTGTGCTCTCGGCAGAAACCCTTCTGGAGAACCCTGCACTTTTTGCTGGGTTTCGAACTGCTGAATGGATAGAGGGCCACGAAGAAAATACCCGGGATGGTAACCTGGATCAAGCGGATCTGCTTGTAGACTACTTGAAGCTTTGTGAAAAATACCCTGTCCCATGGAGAATGATCCGTTCGCATGTACACAAGTTATTGGGAGATTGGTTCAAGATTCATCCACACATTAGAGAAGACTTCAATGCACAATCGAAGCTCACCTTTGAATTTCTTTACAATATGGTGGAGCGGCTGAGAGAATTGCAGGTAAAAATGCCTCTTTATATCAAAGATTCTCATGCACTTGCAATTTCATCCAATGCTTGA

Protein sequence

MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGVLSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISSNA*

Homology

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BLAST of CsGy2G026490.2 vs. ExPASy Swiss-Prot
Match: Q6P1R4 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Homo sapiens OX=9606 GN=DUS1L PE=1 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 4.1e-66
Identity = 121/260 (46.54%), Postives = 166/260 (63.85%), Query Frame = 0


Query: 1   MLHSRIFTENEKYR--NMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLG 60
           MLH+++F  +  YR  N+    C EDRPL VQFCANDP+V ++AA   + YCD +DLNLG
Sbjct: 48  MLHAQVFVRDANYRKENLYCEVCPEDRPLIVQFCANDPEVFVQAALLAQDYCDAIDLNLG 107

Query: 61  CPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDA 120
           CPQ IA+RG+YGAFL D   L++ ++      L VPV+CKIR+FP +  T+ YA+MLE A
Sbjct: 108 CPQMIAKRGHYGAFLQDEWDLLQRMILLAHEKLSVPVTCKIRVFPEIDKTVRYAQMLEKA 167

Query: 121 GCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVE 180
           GC LL VHGRT+++K      A+W  I AV+ AV IPV ANGNI+ ++DV  CL++TGV+
Sbjct: 168 GCQLLTVHGRTKEQKGPLSGAASWEHIKAVRKAVAIPVFANGNIQCLQDVERCLRDTGVQ 227

Query: 181 GVLSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIR 240
           GV+SAE  L NPALF G   A W                +L  +YL +  ++P P   +R
Sbjct: 228 GVMSAEGNLHNPALFEGRSPAVW----------------ELAEEYLDIVREHPCPLSYVR 287

Query: 241 SHVHKLLGDWFKIHPHIRED 259
           +H+ KL     ++H  +RE+
Sbjct: 288 AHLFKLWHHTLQVHQELREE 291

[Close]

BLAST of CsGy2G026490.2 vs. ExPASy Swiss-Prot
Match: Q8K582 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Rattus norvegicus OX=10116 GN=Dus1l PE=2 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 5.3e-66
Identity = 121/260 (46.54%), Postives = 165/260 (63.46%), Query Frame = 0


Query: 1   MLHSRIFTENEKYR--NMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLG 60
           MLH+++F  +  YR  N+    C EDRPL VQFCANDP+V ++AA   + YCD +DLNLG
Sbjct: 48  MLHAQVFVRDANYRKENLYCDVCPEDRPLIVQFCANDPEVFVQAALLAQDYCDAIDLNLG 107

Query: 61  CPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDA 120
           CPQ IA+RG YGAFL +   L++ ++      L VPV+CKIR+FP +  T+ YA+MLE A
Sbjct: 108 CPQMIAKRGRYGAFLQEEWDLLQRMILLAHERLSVPVTCKIRVFPEIDKTVRYAQMLEKA 167

Query: 121 GCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVE 180
           GC LL VHGRT+++K      A+W  I AV+ AV IPV ANGNIR ++DV  C+Q+TGV+
Sbjct: 168 GCQLLTVHGRTKEQKGPMAGTASWEHIKAVRKAVGIPVFANGNIRCLQDVERCIQDTGVQ 227

Query: 181 GVLSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIR 240
           GV+SAE  L NPALF G   A W                +L  +YL +  ++P P   +R
Sbjct: 228 GVMSAEGNLHNPALFEGRSPAVW----------------ELADEYLDIVRQHPCPLSYVR 287

Query: 241 SHVHKLLGDWFKIHPHIRED 259
           +H+ KL     ++H  +RE+
Sbjct: 288 AHLFKLWHHTLQVHQQLREE 291

[Close]

BLAST of CsGy2G026490.2 vs. ExPASy Swiss-Prot
Match: Q8C2P3 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Mus musculus OX=10090 GN=Dus1l PE=2 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 9.0e-66
Identity = 120/260 (46.15%), Postives = 166/260 (63.85%), Query Frame = 0


Query: 1   MLHSRIFTENEKYR--NMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLG 60
           MLH+++F  +  YR  N+    C EDRPL VQFCANDP+V ++AA   + YCD +DLNLG
Sbjct: 48  MLHAQVFVRDANYRKENLYCDVCPEDRPLIVQFCANDPEVFVQAALLAQDYCDAIDLNLG 107

Query: 61  CPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDA 120
           CPQ IA+RG+YGAFL +   L++ ++      L VPV+CKIR+FP +  T+ YA+MLE A
Sbjct: 108 CPQMIAKRGHYGAFLQEEWDLLQRMILLAHERLSVPVTCKIRVFPEIDKTVRYAQMLEKA 167

Query: 121 GCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVE 180
           GC LL VHGRT+++K      A+W  I AV+ AV IPV ANGNI+ ++DV  C+Q+TGV+
Sbjct: 168 GCQLLTVHGRTKEQKGPMAGTASWEHIKAVRKAVGIPVFANGNIQCLQDVERCIQDTGVQ 227

Query: 181 GVLSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIR 240
           GV+SAE  L NPALF G   A W                +L  +YL +  ++P P   +R
Sbjct: 228 GVMSAEGNLHNPALFEGRSPAVW----------------ELAEEYLDIVRQHPCPLSYVR 287

Query: 241 SHVHKLLGDWFKIHPHIRED 259
           +H+ KL     ++H  +RE+
Sbjct: 288 AHLFKLWHHTLQVHQQLREE 291

[Close]

BLAST of CsGy2G026490.2 vs. ExPASy Swiss-Prot
Match: Q9HGN6 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dus1 PE=3 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 6.1e-54
Identity = 115/265 (43.40%), Postives = 164/265 (61.89%), Query Frame = 0


Query: 1   MLHSRIFTENEKYRNMEFT--TCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLG 60
           M HSR+F E+E YRN  F+  T  E+RPL +QFC NDP+++L+AA+   PYCD VD+NLG
Sbjct: 49  MFHSRLFGESEDYRNKVFSTRTIPEERPLIIQFCGNDPEIMLKAAKIAAPYCDAVDVNLG 108

Query: 61  CPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDA 120
           CPQ IA++G YG+FL +N  L+++I+ KL + L +PV+ KIRIFP+ Q T++YA+M+  A
Sbjct: 109 CPQGIAKKGKYGSFLQENWNLIESIITKLHTELSIPVTAKIRIFPDPQKTLDYAKMILKA 168

Query: 121 GCSLLAVHGRTRDEKDGKKFRANWSAISAV-KNAVRIPVL-ANGNIRHMEDVNDCLQETG 180
           G S+LAVHGR R++K      A+W  I  + KN     VL ANGNI H +D++ C++ TG
Sbjct: 169 GASILAVHGRLREQKGHFTGIADWEQIQMLRKNLPSETVLFANGNILHAQDIDRCIKYTG 228

Query: 181 VEGVLSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPV---- 240
           V+GVLSAE  L NP +F               T    +D  D+  +YL +  ++ +    
Sbjct: 229 VDGVLSAEGSLYNPRIFLP-------PSSPLMTLYPRID--DMCEEYLNIIREFKLESDY 288

Query: 241 -PWRMIRSHVHKLLGDWFKIHPHIR 257
                I+ H+ KL+     IH  IR
Sbjct: 289 SSLSAIKGHLFKLMRPLLSIHTDIR 304

[Close]

BLAST of CsGy2G026490.2 vs. ExPASy Swiss-Prot
Match: P53759 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DUS1 PE=1 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 1.6e-49
Identity = 106/278 (38.13%), Postives = 161/278 (57.91%), Query Frame = 0


Query: 1   MLHSRIFTENEKYRNMEFTT---CQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL 60
           MLH+++F  ++KYR   +++      DRPL VQFCANDP+ LL AA+ VE  CD VDLNL
Sbjct: 60  MLHAKLFATSKKYREDNWSSLDGSSVDRPLVVQFCANDPEYLLAAAKLVEDKCDAVDLNL 119

Query: 61  GCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLED 120
           GCPQ IA++G+YG+FLM+   L+  ++  L  NL+VPV+ KIRIF + + ++NYA+M+ D
Sbjct: 120 GCPQGIAKKGHYGSFLMEEWDLIHNLINTLHKNLKVPVTAKIRIFDDCEKSLNYAKMVLD 179

Query: 121 AGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAV--RIPVLANGNIRHMEDVNDCLQET 180
           AG   L VHGR R++K  K   ANW  I  +++ +       ANGNI + ED++ C++  
Sbjct: 180 AGAQFLTVHGRVREQKGQKTGLANWETIKYLRDNLPKETVFFANGNILYPEDISRCMEHI 239

Query: 181 GVEGVLSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLV----DYLKLCEKYP 240
           G + V+SAE  L NP +F   +T           ++    + D ++      +K C++  
Sbjct: 240 GADAVMSAEGNLYNPGVFNVGQT---------KNKEKIFPRVDKIIREYFQIVKECQESK 299

Query: 241 VPWRMIRSHVHKLLGDWFKIHPHIREDF-NAQSKLTFE 269
                ++SH  K+L  +   H  IR       +K T+E
Sbjct: 300 ASKTAMKSHFFKILRPFLPHHTDIRSTLATMNAKATWE 328

[Close]

BLAST of CsGy2G026490.2 vs. NCBI nr
Match: KAE8652323.1 (hypothetical protein Csa_021950 [Cucumis sativus])

HSP 1 Score: 619 bits (1595), Expect = 5.31e-222
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 0


Query: 1   MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 60
           MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP
Sbjct: 106 MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 165

Query: 61  QRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGC 120
           QRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGC
Sbjct: 166 QRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGC 225

Query: 121 SLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGV 180
           SLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGV
Sbjct: 226 SLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGV 285

Query: 181 LSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH 240
           LSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH
Sbjct: 286 LSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH 345

Query: 241 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISSNA 300
           VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISSNA
Sbjct: 346 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISSNA 405

[Close]

BLAST of CsGy2G026490.2 vs. NCBI nr
Match: XP_011650036.1 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucumis sativus])

HSP 1 Score: 619 bits (1595), Expect = 1.14e-221
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 0


Query: 1   MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 60
           MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP
Sbjct: 127 MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 186

Query: 61  QRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGC 120
           QRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGC
Sbjct: 187 QRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGC 246

Query: 121 SLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGV 180
           SLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGV
Sbjct: 247 SLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGV 306

Query: 181 LSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH 240
           LSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH
Sbjct: 307 LSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH 366

Query: 241 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISSNA 300
           VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISSNA
Sbjct: 367 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISSNA 426

[Close]

BLAST of CsGy2G026490.2 vs. NCBI nr
Match: XP_008462837.1 (PREDICTED: tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucumis melo])

HSP 1 Score: 609 bits (1571), Expect = 5.17e-218
Identity = 296/300 (98.67%), Postives = 297/300 (99.00%), Query Frame = 0


Query: 1   MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 60
           MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP
Sbjct: 127 MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 186

Query: 61  QRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGC 120
           QRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGC
Sbjct: 187 QRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGC 246

Query: 121 SLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGV 180
           SLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVN+CLQETGVEGV
Sbjct: 247 SLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQETGVEGV 306

Query: 181 LSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH 240
           LSAETLLENPALFAGFRTAEWIEG EENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH
Sbjct: 307 LSAETLLENPALFAGFRTAEWIEGQEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH 366

Query: 241 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISSNA 300
           VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSH  AISSNA
Sbjct: 367 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHVPAISSNA 426

[Close]

BLAST of CsGy2G026490.2 vs. NCBI nr
Match: XP_038901742.1 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Benincasa hispida])

HSP 1 Score: 602 bits (1552), Expect = 3.63e-215
Identity = 291/300 (97.00%), Postives = 296/300 (98.67%), Query Frame = 0


Query: 1   MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 60
           MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP
Sbjct: 124 MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 183

Query: 61  QRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGC 120
           QRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIF NLQDTINYARMLEDAGC
Sbjct: 184 QRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFSNLQDTINYARMLEDAGC 243

Query: 121 SLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGV 180
           SLLAVHGRTRDEKDGKKFRANWSAISAVKNAVR+PVLANGNIRHMEDV +CLQETGVEGV
Sbjct: 244 SLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRVPVLANGNIRHMEDVYNCLQETGVEGV 303

Query: 181 LSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH 240
           LSAETLLENPALFAGFRTAEWI+GHEENTRDGNLDQADLLVDYLK CEKYPVPWRMIRSH
Sbjct: 304 LSAETLLENPALFAGFRTAEWIKGHEENTRDGNLDQADLLVDYLKFCEKYPVPWRMIRSH 363

Query: 241 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISSNA 300
           VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVK+PLYIKDSHA AI+SNA
Sbjct: 364 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSHASAIASNA 423

[Close]

BLAST of CsGy2G026490.2 vs. NCBI nr
Match: XP_022986543.1 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucurbita maxima])

HSP 1 Score: 587 bits (1514), Expect = 1.99e-209
Identity = 284/298 (95.30%), Postives = 292/298 (97.99%), Query Frame = 0


Query: 1   MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 60
           MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP
Sbjct: 121 MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 180

Query: 61  QRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGC 120
           QRIARRGNYGAFLMDNLPLVKAIVEK+ASNLQVPVSCKIR+FPNLQDTINYARMLEDAGC
Sbjct: 181 QRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGC 240

Query: 121 SLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGV 180
           SLLAVHGRTRDEKDGKKFRANWSAI AVKNA+RIPVLANGNIRHMEDVN+CLQETGVEGV
Sbjct: 241 SLLAVHGRTRDEKDGKKFRANWSAIRAVKNALRIPVLANGNIRHMEDVNNCLQETGVEGV 300

Query: 181 LSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH 240
           LSAETLLENPALFAGFRTA+W  G EE++RDGNLDQADLLVDYLKLCEKYPVPWRMIRSH
Sbjct: 301 LSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH 360

Query: 241 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISS 298
           VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVK+PLYIKDS A AISS
Sbjct: 361 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPASAISS 418

[Close]

BLAST of CsGy2G026490.2 vs. ExPASy TrEMBL
Match: A0A0A0LTG2 (Dus domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G428390 PE=4 SV=1)

HSP 1 Score: 619 bits (1595), Expect = 5.54e-222
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 0


Query: 1   MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 60
           MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP
Sbjct: 127 MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 186

Query: 61  QRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGC 120
           QRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGC
Sbjct: 187 QRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGC 246

Query: 121 SLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGV 180
           SLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGV
Sbjct: 247 SLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGV 306

Query: 181 LSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH 240
           LSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH
Sbjct: 307 LSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH 366

Query: 241 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISSNA 300
           VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISSNA
Sbjct: 367 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISSNA 426

[Close]

BLAST of CsGy2G026490.2 vs. ExPASy TrEMBL
Match: A0A1S3CHU6 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucumis melo OX=3656 GN=LOC103501114 PE=4 SV=1)

HSP 1 Score: 609 bits (1571), Expect = 2.50e-218
Identity = 296/300 (98.67%), Postives = 297/300 (99.00%), Query Frame = 0


Query: 1   MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 60
           MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP
Sbjct: 127 MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 186

Query: 61  QRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGC 120
           QRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGC
Sbjct: 187 QRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGC 246

Query: 121 SLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGV 180
           SLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVN+CLQETGVEGV
Sbjct: 247 SLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQETGVEGV 306

Query: 181 LSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH 240
           LSAETLLENPALFAGFRTAEWIEG EENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH
Sbjct: 307 LSAETLLENPALFAGFRTAEWIEGQEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH 366

Query: 241 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISSNA 300
           VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSH  AISSNA
Sbjct: 367 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHVPAISSNA 426

[Close]

BLAST of CsGy2G026490.2 vs. ExPASy TrEMBL
Match: A0A6J1JGU3 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita maxima OX=3661 GN=LOC111484251 PE=4 SV=1)

HSP 1 Score: 587 bits (1514), Expect = 9.64e-210
Identity = 284/298 (95.30%), Postives = 292/298 (97.99%), Query Frame = 0


Query: 1   MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 60
           MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP
Sbjct: 121 MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 180

Query: 61  QRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGC 120
           QRIARRGNYGAFLMDNLPLVKAIVEK+ASNLQVPVSCKIR+FPNLQDTINYARMLEDAGC
Sbjct: 181 QRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGC 240

Query: 121 SLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGV 180
           SLLAVHGRTRDEKDGKKFRANWSAI AVKNA+RIPVLANGNIRHMEDVN+CLQETGVEGV
Sbjct: 241 SLLAVHGRTRDEKDGKKFRANWSAIRAVKNALRIPVLANGNIRHMEDVNNCLQETGVEGV 300

Query: 181 LSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH 240
           LSAETLLENPALFAGFRTA+W  G EE++RDGNLDQADLLVDYLKLCEKYPVPWRMIRSH
Sbjct: 301 LSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH 360

Query: 241 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISS 298
           VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVK+PLYIKDS A AISS
Sbjct: 361 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPASAISS 418

[Close]

BLAST of CsGy2G026490.2 vs. ExPASy TrEMBL
Match: A0A6J1FWR0 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita moschata OX=3662 GN=LOC111448913 PE=4 SV=1)

HSP 1 Score: 586 bits (1510), Expect = 3.92e-209
Identity = 283/298 (94.97%), Postives = 293/298 (98.32%), Query Frame = 0


Query: 1   MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 60
           MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP
Sbjct: 121 MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 180

Query: 61  QRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGC 120
           QRIARRGNYGAFLMDNLPLVKAIVEK+ASNLQVPVSCKIR+FPNLQDTINYARMLEDAGC
Sbjct: 181 QRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGC 240

Query: 121 SLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGV 180
           SLLAVHGRTRDEKDGKKFRANWSAISAVKNA+RIPVLANGNIRHMEDV++CLQETGVEGV
Sbjct: 241 SLLAVHGRTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGVEGV 300

Query: 181 LSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH 240
           LSAETLLENPALFAGFRTA+W  G EE++RDGNLDQADLLVDYLKLCEKYPVPWRMIRSH
Sbjct: 301 LSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH 360

Query: 241 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISS 298
           VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVE+LRELQVK+PLYIKDS A AISS
Sbjct: 361 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVEQLRELQVKIPLYIKDSPASAISS 418

[Close]

BLAST of CsGy2G026490.2 vs. ExPASy TrEMBL
Match: A0A6J1L641 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita maxima OX=3661 GN=LOC111499526 PE=4 SV=1)

HSP 1 Score: 581 bits (1498), Expect = 1.23e-207
Identity = 282/301 (93.69%), Postives = 294/301 (97.67%), Query Frame = 0


Query: 1   MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 60
           MLHSRIFTEN+KYRN+EFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP
Sbjct: 99  MLHSRIFTENDKYRNVEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 158

Query: 61  QRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGC 120
           QRIA+RGNYGAFLMDNLPLVKAIVEKLASNL+VPVSCKIRIFPNLQDTINYARMLEDAGC
Sbjct: 159 QRIAKRGNYGAFLMDNLPLVKAIVEKLASNLEVPVSCKIRIFPNLQDTINYARMLEDAGC 218

Query: 121 SLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGV 180
           SLLAVHGRTRDEKDGKKFRANWSAI+AVKNAVRIPVLANGNIRHMEDV DCLQETGVEGV
Sbjct: 219 SLLAVHGRTRDEKDGKKFRANWSAITAVKNAVRIPVLANGNIRHMEDVYDCLQETGVEGV 278

Query: 181 LSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH 240
           LSAETLLENPALFAGFRT EWIEG EE+TRDGNLDQADLLV+YLKLCEKYPVPWR+IRSH
Sbjct: 279 LSAETLLENPALFAGFRTVEWIEGCEESTRDGNLDQADLLVEYLKLCEKYPVPWRIIRSH 338

Query: 241 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSH-ALAISSN 300
           VHKLLGDWFK+HPHIREDFNAQSKLTFEFLYNMVERLRELQVK+PLYIKDS  A AI +N
Sbjct: 339 VHKLLGDWFKLHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSSSASAILAN 398

[Close]

BLAST of CsGy2G026490.2 vs. TAIR 10
Match: AT5G67220.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 500.4 bits (1287), Expect = 1.0e-141
Identity = 233/290 (80.34%), Postives = 263/290 (90.69%), Query Frame = 0


Query: 1   MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 60
           MLHSRIFTE EKYRN EFTTC+EDRPLFVQFCANDPD LLEAA+RVEPYCDYVD+NLGCP
Sbjct: 119 MLHSRIFTETEKYRNQEFTTCKEDRPLFVQFCANDPDTLLEAAKRVEPYCDYVDINLGCP 178

Query: 61  QRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGC 120
           QRIARRGNYGAFLMDNLPLVK++VEKLA NL VPVSCKIRIFPNL+DT+ YA+MLEDAGC
Sbjct: 179 QRIARRGNYGAFLMDNLPLVKSLVEKLAQNLNVPVSCKIRIFPNLEDTLKYAKMLEDAGC 238

Query: 121 SLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGV 180
           SLLAVHGRTRDEKDGKKFRA+WSAI  VKNA+RIPVLANGN+R +EDV++C++ETGVEGV
Sbjct: 239 SLLAVHGRTRDEKDGKKFRADWSAIKEVKNAMRIPVLANGNVRCIEDVDNCIKETGVEGV 298

Query: 181 LSAETLLENPALFAGFRTAEWI-EGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRS 240
           LSAETLLENPA FAGFRTAEW  +  EE   DG LDQ DL+V+YLKLCEK+PVPWRMIRS
Sbjct: 299 LSAETLLENPAAFAGFRTAEWAKDNEEEGFVDGGLDQGDLVVEYLKLCEKHPVPWRMIRS 358

Query: 241 HVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIK 290
           HVHK+LG+WF+IHP +RE  NAQ+ LTFEFLY +V++LREL  ++PLY K
Sbjct: 359 HVHKMLGEWFRIHPQVREQLNAQNILTFEFLYGLVDQLRELGGRVPLYKK 408

[Close]

BLAST of CsGy2G026490.2 vs. TAIR 10
Match: AT3G49640.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 111.7 bits (278), Expect = 1.0e-24
Identity = 64/188 (34.04%), Postives = 99/188 (52.66%), Query Frame = 0


Query: 15  NMEFTTCQEDRPLFV-QFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFL 74
           N+ F+TC E++   V Q   +D    L+A+  V      +D+N+GCP+  + +G  GA L
Sbjct: 65  NVVFSTCDEEKSRVVFQMGTSDAVRALKASEIVCNDVATIDINMGCPKAFSIQGGMGAAL 124

Query: 75  MDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEK 134
           +    L+  I+  L  NL VPV+CKIR+  +  DT+  AR +E  G   LAVHG  R   
Sbjct: 125 LSKPELIHDILATLKRNLDVPVTCKIRLLKSPADTVELARRIEKLGVPALAVHG--RKIA 184

Query: 135 DGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGVLSAETLLENPALF 194
           D  +  A W  I+ V  A+ IPV+ANG++   +D +     TG   V+ A   + N ++F
Sbjct: 185 DRPRDPAKWDEIADVVAALSIPVIANGDVLEYDDFSRIKTATGAASVMVARGAMWNASIF 244

Query: 195 AGFRTAEW 202
           +    + W
Sbjct: 245 SPKGKSHW 250

[Close]

BLAST of CsGy2G026490.2 vs. TAIR 10
Match: AT3G49640.2 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 111.7 bits (278), Expect = 1.0e-24
Identity = 64/188 (34.04%), Postives = 99/188 (52.66%), Query Frame = 0


Query: 15  NMEFTTCQEDRPLFV-QFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFL 74
           N+ F+TC E++   V Q   +D    L+A+  V      +D+N+GCP+  + +G  GA L
Sbjct: 26  NVVFSTCDEEKSRVVFQMGTSDAVRALKASEIVCNDVATIDINMGCPKAFSIQGGMGAAL 85

Query: 75  MDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEK 134
           +    L+  I+  L  NL VPV+CKIR+  +  DT+  AR +E  G   LAVHG  R   
Sbjct: 86  LSKPELIHDILATLKRNLDVPVTCKIRLLKSPADTVELARRIEKLGVPALAVHG--RKIA 145

Query: 135 DGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGVLSAETLLENPALF 194
           D  +  A W  I+ V  A+ IPV+ANG++   +D +     TG   V+ A   + N ++F
Sbjct: 146 DRPRDPAKWDEIADVVAALSIPVIANGDVLEYDDFSRIKTATGAASVMVARGAMWNASIF 205

Query: 195 AGFRTAEW 202
           +    + W
Sbjct: 206 SPKGKSHW 211

[Close]

BLAST of CsGy2G026490.2 vs. TAIR 10
Match: AT4G38890.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 65.5 bits (158), Expect = 8.5e-11
Identity = 40/147 (27.21%), Postives = 74/147 (50.34%), Query Frame = 0


Query: 29  VQFCANDPDVLLEAARRVEPYC--DYVDLNLGCPQRIARRGNYGAFLMDNLPLVKAIVEK 88
           VQ C + PD +      ++  C  D++D+N+GCP  +    + G+ L++    +K IVE 
Sbjct: 397 VQICGSYPDTVSRVVELIDRECTVDFIDINMGCPIDMVVNKSAGSALLNKPLRMKNIVEV 456

Query: 89  LASNLQVPVSCKIR--IFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRANWSA 148
            +S ++ P++ K+R   F       +    + + G + + +HGR+R ++  K   A+W  
Sbjct: 457 SSSIVETPITIKVRTAFFEGKNRIDSLIADIGNWGATAVTIHGRSRQQRYSKS--ADWDY 516

Query: 149 I-SAVKNA-VRIPVLANGNIRHMEDVN 170
           I    KNA   + V+ NG++    D N
Sbjct: 517 IYQCTKNATTNLQVIGNGDVYSYLDWN 541

[Close]

BLAST of CsGy2G026490.2 vs. TAIR 10
Match: AT3G63510.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 51.2 bits (121), Expect = 1.7e-06
Identity = 54/195 (27.69%), Postives = 77/195 (39.49%), Query Frame = 0


Query: 23  EDRPLFVQFCANDPDVLLEAARRVEPY-CDYVDLNLGCPQ-RIARRGNYGAFLMDNLPLV 82
           +  P+ +Q   ++ + L +AA+  + Y  D ++LN GCP  ++A  G +G  LM    LV
Sbjct: 106 QQHPIVLQLGGSNVENLAKAAKLSDAYGYDEINLNCGCPSPKVAGHGCFGVSLMLKPKLV 165

Query: 83  KAIVEKLASNLQVPVSCKIRI-------FPNLQDTINYARMLEDAGCSLLAVHGRTRDEK 142
              +  +A+N  VPV+ K RI       +  L D I     L         VH R     
Sbjct: 166 GEAMSAIAANTNVPVTVKCRIGVDNHDSYDELCDFIYKVSTLSPT--RHFIVHSR----- 225

Query: 143 DGKKFRANWSAISAVKNAVRIPVL------------------ANGNIRHMEDVNDCLQET 191
                +A    IS   N  RIP L                   NG I  +  VN  L+E 
Sbjct: 226 -----KALLGGISPADNR-RIPPLKYEYYYALVRDFPDLRFTINGGITSVSKVNAALKE- 285

The following BLAST results are available for this feature:

BLAST of CsGy2G026490.2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6P1R4	4.1e-66	46.54	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Homo sapiens OX=9606 GN=...	[more]
Q8K582	5.3e-66	46.54	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Rattus norvegicus OX=101...	[more]
Q8C2P3	9.0e-66	46.15	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Mus musculus OX=10090 GN...	[more]
Q9HGN6	6.1e-54	43.40	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS=Schizosaccharomyces pombe (st...	[more]
P53759	1.6e-49	38.13	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS=Saccharomyces cerevisiae (str...	[more]

BLAST of CsGy2G026490.2 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8652323.1	5.31e-222	100.00	hypothetical protein Csa_021950 [Cucumis sativus]	[more]
XP_011650036.1	1.14e-221	100.00	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucumis sativus]	[more]
XP_008462837.1	5.17e-218	98.67	PREDICTED: tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucumis melo]	[more]
XP_038901742.1	3.63e-215	97.00	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Benincasa hispida]	[more]
XP_022986543.1	1.99e-209	95.30	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucurbita maxima]	[more]

BLAST of CsGy2G026490.2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LTG2	5.54e-222	100.00	Dus domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G428390 PE=4 SV...	[more]
A0A1S3CHU6	2.50e-218	98.67	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucumis melo OX=3656 GN=...	[more]
A0A6J1JGU3	9.64e-210	95.30	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita maxima OX=3661...	[more]
A0A6J1FWR0	3.92e-209	94.97	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita moschata OX=36...	[more]
A0A6J1L641	1.23e-207	93.69	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita maxima OX=3661...	[more]

BLAST of CsGy2G026490.2 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G67220.1	1.0e-141	80.34	FMN-linked oxidoreductases superfamily protein	[more]
AT3G49640.1	1.0e-24	34.04	Aldolase-type TIM barrel family protein	[more]
AT3G49640.2	1.0e-24	34.04	Aldolase-type TIM barrel family protein	[more]
AT4G38890.1	8.5e-11	27.21	FMN-linked oxidoreductases superfamily protein	[more]
AT3G63510.1	1.7e-06	27.69	FMN-linked oxidoreductases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001269	tRNA-dihydrouridine synthase	PIRSF	PIRSF006621	Dus	coord: 1..287 e-value: 1.7E-57 score: 192.8
IPR035587	DUS-like, FMN-binding domain	PFAM	PF01207	Dus	coord: 1..264 e-value: 5.2E-63 score: 213.0
IPR035587	DUS-like, FMN-binding domain	CDD	cd02801	DUS_like_FMN	coord: 1..201 e-value: 3.16143E-86 score: 256.267
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 1..202 e-value: 2.1E-64 score: 219.0
None	No IPR available	PANTHER	PTHR11082	TRNA-DIHYDROURIDINE SYNTHASE	coord: 1..289
None	No IPR available	PANTHER	PTHR11082:SF35	BNAA09G07510D PROTEIN	coord: 1..289
None	No IPR available	SUPERFAMILY	51395	FMN-linked oxidoreductases	coord: 2..246
IPR018517	tRNA-dihydrouridine synthase, conserved site	PROSITE	PS01136	UPF0034	coord: 53..71

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CsGy2G026490	CsGy2G026490	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CsGy2G026490.2.utr5p1	CsGy2G026490.2.utr5p1	five_prime_UTR
CsGy2G026490.2.utr5p2	CsGy2G026490.2.utr5p2	five_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CsGy2G026490.2.exon1	CsGy2G026490.2.exon1	exon
CsGy2G026490.2.exon2	CsGy2G026490.2.exon2	exon
CsGy2G026490.2.exon3	CsGy2G026490.2.exon3	exon
CsGy2G026490.2.exon4	CsGy2G026490.2.exon4	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.CsGy2G026490.2	cds.CsGy2G026490.2	CDS
cds.CsGy2G026490.2	cds.CsGy2G026490.2_2	CDS
cds.CsGy2G026490.2	cds.CsGy2G026490.2_3	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CsGy2G026490.2.utr3p1	CsGy2G026490.2.utr3p1	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CsGy2G026490.2	CsGy2G026490.2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008033	tRNA processing
biological_process	GO:0002943	tRNA dihydrouridine synthesis
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0017150	tRNA dihydrouridine synthase activity