CsGy1G013430.2 (mRNA) Cucumber (Gy14) v2.1

Overview
NameCsGy1G013430.2
TypemRNA
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description4-coumarate-CoA ligase
LocationGy14Chr1: 8812595 .. 8816920 (-)
Sequence length3198
RNA-Seq ExpressionCsGy1G013430.2
SyntenyCsGy1G013430.2
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTAGTTGGCATTATTTGTAAAAGATGGGTCATTTTAGATATTAACGGACAAATTTCCAAATTTTTTATCACCTTCCTCTTTACCGGTCAAGGTTGAAGACGAAATCTCCAAATCTTCCCATTACCTACCACTCATGGCGACACTTTTTTCTTCCCTCGTGCTGCTGTAACAAGTCTTCACTCCTTATACAAGTCTCTCCTTATTTTGCTTCCATTTAACCGACTTTTGATGATGCCTCATTTTTCTCATATTCCTTGTCATCTTCACTTTCCTTTTCTCCCTCAAATTCCGCATCGGTCTGTGTGCTACTACTACTTATCCAAACCCTCTCTGCTAATACCTTTGTAATTTTCATTCAATGGAGAAATCTGGGTATGGTCGCGATGGAATTTTCCGGTCGTTACGGCCTCCGCTTGTCTTTCCTAAAGATCCCAATCTCTCCATGATTTCTTTCCTCTTCAGAAATTTATTGTCTTATCCAAATAGACTAGCCATTGTTGATGCGGAATCTTCAAATTCTGTCTCGTATTCCCAGCTTAAAGCCTTGGCAATTAGGGTATCCAATGGTTTGATCCAATTGGGTATTGAGAAAAATGACGTGGTGTTGATTTTTGCTCCAAATTCAGTTCAATTCACCATTTGTTTCATTGGGGTTATTGCCATTGGGGCTATTGTAACTACTTGTAATCCTGTATACACGGTTTCTGAGCTAACTAAGCAAGTTAGAGATGCTAAACCTAAACTCGTGATATCTGTTGCTGAATTGTGGGATAAAGTGAAGAATCTAAACATCCCAACTGTGTTACTAGACCAGCAAATTCCTTCAGCAATTCATTCTCCTAAAATATTGTGTTTCAATGATTTGGTGAACATGGCTGGTGATAAATCTGGGTCTGAGTTTCCAATTGTTGGGGTGAAGCAGAGTGATACAGCTGCACTTCTATATTCATCAGGAACAACAGGAGCTAGTAAGGGTGTGATTTTGACTCACGGAAACTTCATAGCATCTTCTTTGATGATAACAATGGATCAGACGTTTAATGGAGAGGAACATGGCGTATTTCTGAACTTCTTGCCAATGTTTCATGTGTTTGGATTGGCTTGTATAACATATGCTCAACTGCAGAAAGGAAACACCGTTGTCTCTATGCCAAAGTTTAATCTTGAGAAAGCCTTGTGGGCAGTGGAGAAGTACAAGGTGACAGATCTGTGGGTTGTGCCGCCTGTGGTGCTTGCTTTGGCAAAGCAGAGTCTGGTGAAGAAATACAACCTTTCATCCGTGAAGCGTGTCGGTTCAGGGGCTGCACCTCTTGGGAGGGAATTAATGGAGGAATGTGCTAATAACATTCCCAGTGCTGTGGTTATCCAGGTACCCTGTTGTAGGCTGTGTGTGTTCTATAACATGTTTTGTGTATTGTTTCTTGTTGGCTAAAATCAGCTTATCAGATTACAGAGGTAATTACTAAATCACCCTGATTGTTTTTAGAAATGTTCAAAATCTCTGACGGTGATAAAAACAATGAAAATGCTTTCAAGTTATTTTATTCGGTTAAAAATACCTCCCCTGTTCTAAGTCAAATACAATCAACTTAAATGTCGACTACTCCTGAATGGAAATTATTGTGGTTCTCACTTCATTTGACTTTGATTTACAACCTCGTAGAATTGAATTATTACATTCTAATGTAATGAACCGTTGAATTTGTTATCATTTTCAGGGTTATGGTATGACTGAGACTTGTGGAGTTGTTGCTTTGGAGAATCCAGCAGTTGGCAAACGAAATTCTGGTTCAGCTGGAACACTTGCCTCTGGTGTTGAGGCTAAAATAGTCAGTGTAGATACTCTAAAGCCTCTACCTCCTAATCAATATGGCGAGATACATGTTCGAGGACCAAACATGATGCTCGGTATGATTGTGATTATCTTTTCATTGATTTTATGGTTTCATACGATACTTTGGAGAGAGTGTAAGCGTAGTCCCTGCATTTGTTTCAGTTAGAGTAACTATTATCATGAGCTAAGGTGATCTTTCCCATGTTAGGAGCCATTATTAGTACCTATGATTAAAGTCGGTTTTGAAATTCAATATTGAAATACCTCATTTACAGGTTATTTCAACAATCCAGAAGCCACCAAGCAAACTATTGATAAACATGGATGGGTGCATACTGGAGACCTTGGATACTTCGATGAAAATGGTCAGCTTTATGTTGTCGACCGGATCAAGGAACTCATCAAGTACAAAGGTTTTCAGGTGAATATATGATATGTTTATCTTATCATATTTAGTTACGATCTGTACATTTTTAAAATTGAAGTAACATGAGCCGAGCTTCTTTGTTGCTATAACTAACATTGTTGTTGAGATGATACTGCATTCAACCGTTACCTTTCAAAACTTTCTTGCGACACTAATGACATATTTTTCAGTAATTTTGAAATAGTTAAAACCAGACTGACACTGAAACTTGGTTTTGAATAGTAAAAGATATACATACACTTCAAAGTAATTTTAACAATTTCAAAACCATCCACAAACATGTCCTAATATCCGGTAATTTCTCTATCGATGATAGCATGCTAGGTTAGTCGTTGTATGTGCAAATGCTGTTTGGAGTTCAAGCTTTTCTCTCTCTCTCATTTTTTGTTACTATATCTCCAGGTTGCACCAGCTGAGCTTGAAGCACTGCTGGTTTCTCACCCTGAGATACTAGATGCTGTTGTCATCCCGTAAGCTTTCATTACTCATATTTAAACTTTTGAAGTTCATTTCTTAGTAACCATTTAACTGATACTATCAATTTTGAAACTTAATCGACAGCTGCCCTGATGAAGAAGCTGGAGAAGTTCCAATTGCTTATGTTGTTCGATCTGCCAATAGTTCTTTGACCGAAGAGGACATCCTTAAATTCATTGCTGATCAGGTAAGCCACTTTAGAAGAAAGTTTACTGTTGTTCAACGTACATCATGAATTAAACTTACTTGTCCTTCTCTTGTAACAGGTTTCTCCTTATAAAAGGTTAAGAAGAGTGACATTCATCAGCAGTGTCCCCAAGTCTGTTTCTGGTAAAATTCTGAGGAGAGAACTCATTGAGAAAGTAAGAGCTAAAATCTGAGATCTTTGTTTCCTAGTTTCATTATGATAGTGGCCATAGAAGAGAAGCAATAAGTTGGTCTATAGACCTAATATTAAATTCATTTTGAGTGAATCTGAATAACTTGACTATAATAGTGAATAAATTATCTCTTTTTATTACAATATGTGTGTGAAAAGAGTGAAAAGACTCACTACTCAATAGTTATCTCGTTTGACATCCATTTGGTTTTTCAAAATTAAACCTTCAAATTTCACTTCCATCCATCGGTTTTCAACTTTTTGTTGCCTATATTTTATTAGTGTTTCTTAAAACCAAATTGGTTTTAAAACGTTGTTTTATTTTTGGATTTTGGTGGTAAAGAAGATGAAATGGAGTCTCCACCATCTTTGAAATTTGAATATTGACTCCAACTATTGTTGGAGAAGTTACACAGTTTAATTCTTTACAAGATCTATTTTGTGTTGGACTATTATACAGTTTTGAACTTCCGTACCTCATTCTGTTTATGATCTACTTGTCTCAAAATGATCTAATCATTAATTTGCCATATGATACAGTTAATTAAGAAATGCTTTTTTTTAGTACAAGTTGGGATGAGGAATTTGAACCATGGATGTCATGGTTCTTAGCAAAACGAATATCAATTGAGTTAAGATGTTGTTTGTTTCTAGCATTACTCCATGCCTCTATGGTCATAGGAAAAAGGAAAATAGACTTCGAGAATTATTGGTGTACCACTCAATCTTTTTACCAACTTTAAATGTGAATTTATTTTCTCCACCTTAAATGTGACACCTAGAAATTGCTTGTACATCAAGGACCCACTTTAGTTAATTTAGAGAGTGGAAAATTTGATGGGTGTTTTCAATAGTGCATTTAATACAACGATTGTTTAAAAAATACACATTCTTTTAAGATATATCAATGCATCCCTTGTCTTTTTTCTAGTACAATTTATTGTTTGACTTTGAATAGTTGATATAAATATACCATCAAATCATAAAACAAAACAAACCAACCACGAAAGACGTCATTTCATATCACGTGTATTTAAAATAGCTTCAAAGATTCGAGAAGTGCTTAATTCATATGACTTTGAGTGTACTAATGACAATCAAGTCTATGGTTTGAAACTTGCATCTCCAATTATTATATTAAAAGAATCTTGAAGAGAATGTCAAAGAGACTCTTATTCTACAGGACTTGCGAATATTTATGGATGAAC

mRNA sequence

GTTAGTTGGCATTATTTGTAAAAGATGGGTCATTTTAGATATTAACGGACAAATTTCCAAATTTTTTATCACCTTCCTCTTTACCGGTCAAGGTTGAAGACGAAATCTCCAAATCTTCCCATTACCTACCACTCATGGCGACACTTTTTTCTTCCCTCGTGCTGCTGTAACAAGTCTTCACTCCTTATACAAGTCTCTCCTTATTTTGCTTCCATTTAACCGACTTTTGATGATGCCTCATTTTTCTCATATTCCTTGTCATCTTCACTTTCCTTTTCTCCCTCAAATTCCGCATCGGTCTGTGTGCTACTACTACTTATCCAAACCCTCTCTGCTAATACCTTTGTAATTTTCATTCAATGGAGAAATCTGGGTATGGTCGCGATGGAATTTTCCGGTCGTTACGGCCTCCGCTTGTCTTTCCTAAAGATCCCAATCTCTCCATGATTTCTTTCCTCTTCAGAAATTTATTGTCTTATCCAAATAGACTAGCCATTGTTGATGCGGAATCTTCAAATTCTGTCTCGTATTCCCAGCTTAAAGCCTTGGCAATTAGGGTATCCAATGGTTTGATCCAATTGGGTATTGAGAAAAATGACGTGGTGTTGATTTTTGCTCCAAATTCAGTTCAATTCACCATTTGTTTCATTGGGGTTATTGCCATTGGGGCTATTGTAACTACTTGTAATCCTGTATACACGGTTTCTGAGCTAACTAAGCAAGTTAGAGATGCTAAACCTAAACTCGTGATATCTGTTGCTGAATTGTGGGATAAAGTGAAGAATCTAAACATCCCAACTGTGTTACTAGACCAGCAAATTCCTTCAGCAATTCATTCTCCTAAAATATTGTGTTTCAATGATTTGGTGAACATGGCTGGTGATAAATCTGGGTCTGAGTTTCCAATTGTTGGGGTGAAGCAGAGTGATACAGCTGCACTTCTATATTCATCAGGAACAACAGGAGCTAGTAAGGGTGTGATTTTGACTCACGGAAACTTCATAGCATCTTCTTTGATGATAACAATGGATCAGACGTTTAATGGAGAGGAACATGGCGTATTTCTGAACTTCTTGCCAATGTTTCATGTGTTTGGATTGGCTTGTATAACATATGCTCAACTGCAGAAAGGAAACACCGTTGTCTCTATGCCAAAGTTTAATCTTGAGAAAGCCTTGTGGGCAGTGGAGAAGTACAAGGTGACAGATCTGTGGGTTGTGCCGCCTGTGGTGCTTGCTTTGGCAAAGCAGAGTCTGGTGAAGAAATACAACCTTTCATCCGTGAAGCGTGTCGGTTCAGGGGCTGCACCTCTTGGGAGGGAATTAATGGAGGAATGTGCTAATAACATTCCCAGTGCTGTGGTTATCCAGGGTTATGGTATGACTGAGACTTGTGGAGTTGTTGCTTTGGAGAATCCAGCAGTTGGCAAACGAAATTCTGGTTCAGCTGGAACACTTGCCTCTGGTGTTGAGGCTAAAATAGTCAGTGTAGATACTCTAAAGCCTCTACCTCCTAATCAATATGGCGAGATACATGTTCGAGGACCAAACATGATGCTCGGTTATTTCAACAATCCAGAAGCCACCAAGCAAACTATTGATAAACATGGATGGGTGCATACTGGAGACCTTGGATACTTCGATGAAAATGGTCAGCTTTATGTTGTCGACCGGATCAAGGAACTCATCAAGTACAAAGGTTTTCAGGTTGCACCAGCTGAGCTTGAAGCACTGCTGGTTTCTCACCCTGAGATACTAGATGCTGTTGTCATCCCCTGCCCTGATGAAGAAGCTGGAGAAGTTCCAATTGCTTATGTTGTTCGATCTGCCAATAGTTCTTTGACCGAAGAGGACATCCTTAAATTCATTGCTGATCAGGTTTCTCCTTATAAAAGGTTAAGAAGAGTGACATTCATCAGCAGTGTCCCCAAGTCTGTTTCTGGTAAAATTCTGAGGAGAGAACTCATTGAGAAAGTAAGAGCTAAAATCTGAGATCTTTGTTTCCTAGTTTCATTATGATAGTGGCCATAGAAGAGAAGCAATAAGTTGGTCTATAGACCTAATATTAAATTCATTTTGAGTGAATCTGAATAACTTGACTATAATAGTGAATAAATTATCTCTTTTTATTACAATATGTGTGTGAAAAGAGTGAAAAGACTCACTACTCAATAGTTATCTCGTTTGACATCCATTTGGTTTTTCAAAATTAAACCTTCAAATTTCACTTCCATCCATCGGTTTTCAACTTTTTGTTGCCTATATTTTATTAGTGTTTCTTAAAACCAAATTGGTTTTAAAACGTTGTTTTATTTTTGGATTTTGGTGGTAAAGAAGATGAAATGGAGTCTCCACCATCTTTGAAATTTGAATATTGACTCCAACTATTGTTGGAGAAGTTACACAGTTTAATTCTTTACAAGATCTATTTTGTGTTGGACTATTATACAGTTTTGAACTTCCGTACCTCATTCTGTTTATGATCTACTTGTCTCAAAATGATCTAATCATTAATTTGCCATATGATACAGTTAATTAAGAAATGCTTTTTTTTAGTACAAGTTGGGATGAGGAATTTGAACCATGGATGTCATGGTTCTTAGCAAAACGAATATCAATTGAGTTAAGATGTTGTTTGTTTCTAGCATTACTCCATGCCTCTATGGTCATAGGAAAAAGGAAAATAGACTTCGAGAATTATTGGTGTACCACTCAATCTTTTTACCAACTTTAAATGTGAATTTATTTTCTCCACCTTAAATGTGACACCTAGAAATTGCTTGTACATCAAGGACCCACTTTAGTTAATTTAGAGAGTGGAAAATTTGATGGGTGTTTTCAATAGTGCATTTAATACAACGATTGTTTAAAAAATACACATTCTTTTAAGATATATCAATGCATCCCTTGTCTTTTTTCTAGTACAATTTATTGTTTGACTTTGAATAGTTGATATAAATATACCATCAAATCATAAAACAAAACAAACCAACCACGAAAGACGTCATTTCATATCACGTGTATTTAAAATAGCTTCAAAGATTCGAGAAGTGCTTAATTCATATGACTTTGAGTGTACTAATGACAATCAAGTCTATGGTTTGAAACTTGCATCTCCAATTATTATATTAAAAGAATCTTGAAGAGAATGTCAAAGAGACTCTTATTCTACAGGACTTGCGAATATTTATGGATGAAC

Coding sequence (CDS)

ATGGAGAAATCTGGGTATGGTCGCGATGGAATTTTCCGGTCGTTACGGCCTCCGCTTGTCTTTCCTAAAGATCCCAATCTCTCCATGATTTCTTTCCTCTTCAGAAATTTATTGTCTTATCCAAATAGACTAGCCATTGTTGATGCGGAATCTTCAAATTCTGTCTCGTATTCCCAGCTTAAAGCCTTGGCAATTAGGGTATCCAATGGTTTGATCCAATTGGGTATTGAGAAAAATGACGTGGTGTTGATTTTTGCTCCAAATTCAGTTCAATTCACCATTTGTTTCATTGGGGTTATTGCCATTGGGGCTATTGTAACTACTTGTAATCCTGTATACACGGTTTCTGAGCTAACTAAGCAAGTTAGAGATGCTAAACCTAAACTCGTGATATCTGTTGCTGAATTGTGGGATAAAGTGAAGAATCTAAACATCCCAACTGTGTTACTAGACCAGCAAATTCCTTCAGCAATTCATTCTCCTAAAATATTGTGTTTCAATGATTTGGTGAACATGGCTGGTGATAAATCTGGGTCTGAGTTTCCAATTGTTGGGGTGAAGCAGAGTGATACAGCTGCACTTCTATATTCATCAGGAACAACAGGAGCTAGTAAGGGTGTGATTTTGACTCACGGAAACTTCATAGCATCTTCTTTGATGATAACAATGGATCAGACGTTTAATGGAGAGGAACATGGCGTATTTCTGAACTTCTTGCCAATGTTTCATGTGTTTGGATTGGCTTGTATAACATATGCTCAACTGCAGAAAGGAAACACCGTTGTCTCTATGCCAAAGTTTAATCTTGAGAAAGCCTTGTGGGCAGTGGAGAAGTACAAGGTGACAGATCTGTGGGTTGTGCCGCCTGTGGTGCTTGCTTTGGCAAAGCAGAGTCTGGTGAAGAAATACAACCTTTCATCCGTGAAGCGTGTCGGTTCAGGGGCTGCACCTCTTGGGAGGGAATTAATGGAGGAATGTGCTAATAACATTCCCAGTGCTGTGGTTATCCAGGGTTATGGTATGACTGAGACTTGTGGAGTTGTTGCTTTGGAGAATCCAGCAGTTGGCAAACGAAATTCTGGTTCAGCTGGAACACTTGCCTCTGGTGTTGAGGCTAAAATAGTCAGTGTAGATACTCTAAAGCCTCTACCTCCTAATCAATATGGCGAGATACATGTTCGAGGACCAAACATGATGCTCGGTTATTTCAACAATCCAGAAGCCACCAAGCAAACTATTGATAAACATGGATGGGTGCATACTGGAGACCTTGGATACTTCGATGAAAATGGTCAGCTTTATGTTGTCGACCGGATCAAGGAACTCATCAAGTACAAAGGTTTTCAGGTTGCACCAGCTGAGCTTGAAGCACTGCTGGTTTCTCACCCTGAGATACTAGATGCTGTTGTCATCCCCTGCCCTGATGAAGAAGCTGGAGAAGTTCCAATTGCTTATGTTGTTCGATCTGCCAATAGTTCTTTGACCGAAGAGGACATCCTTAAATTCATTGCTGATCAGGTTTCTCCTTATAAAAGGTTAAGAAGAGTGACATTCATCAGCAGTGTCCCCAAGTCTGTTTCTGGTAAAATTCTGAGGAGAGAACTCATTGAGAAAGTAAGAGCTAAAATCTGA

Protein sequence

MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQLKALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTKQVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSEFPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLPMFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLVKKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVRAKI*
Homology
BLAST of CsGy1G013430.2 vs. ExPASy Swiss-Prot
Match: M4IRL6 (Probable CoA ligase CCL7 OS=Humulus lupulus OX=3486 GN=CCL7 PE=2 SV=1)

HSP 1 Score: 771.9 bits (1992), Expect = 4.7e-222
Identity = 384/546 (70.33%), Postives = 466/546 (85.35%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDG+FRSLRPPLV PKD NLSM+SF+FRN  SYP + A++D++++ ++S+SQ 
Sbjct: 1   MEKSGYGRDGVFRSLRPPLVLPKDHNLSMVSFVFRNSSSYPQKPALIDSDTNETLSFSQF 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           K++ I+VS+G + LG++KNDVVLIFAPNS+   +CF+G++A GAI TT NP+YTVSEL+K
Sbjct: 61  KSMVIKVSHGFLNLGVQKNDVVLIFAPNSIHVPVCFLGIVASGAIATTSNPLYTVSELSK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLL--DQQIPSAIHS-PKILCFNDLVNMAGDKS 180
           QV+D+ PKL+++V EL++KVK  N+PT+L+  + +  S + S  K+L F+DLV ++G   
Sbjct: 121 QVKDSNPKLIVTVPELFEKVKGFNLPTILIGPNSEDSSPLKSRAKVLTFHDLVTLSGPV- 180

Query: 181 GSEFPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLN 240
            S+FP+V  KQSDTAALLYSSGTTG SKGV+L+H NFIASSLM+TM+Q   GE H VFL 
Sbjct: 181 -SDFPMVDFKQSDTAALLYSSGTTGMSKGVVLSHKNFIASSLMVTMEQDQAGEMHNVFLC 240

Query: 241 FLPMFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQ 300
           FLPMFHVFGLA ITYAQLQ+GNTV+SM +F+LEK L  VEKYKVT LWVVPPV+LAL K 
Sbjct: 241 FLPMFHVFGLAIITYAQLQRGNTVISMARFDLEKILKDVEKYKVTHLWVVPPVILALTKN 300

Query: 301 SLVKKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGK 360
           S+VKKY+LSS+K +GSGAAPLG++LMEECA  +P  +V QGYGMTETCG+V++E+   GK
Sbjct: 301 SIVKKYDLSSLKHIGSGAAPLGKDLMEECAKIVPHGIVAQGYGMTETCGIVSVEDTRGGK 360

Query: 361 RNSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHG 420
           R++GSAG L+SGVEA+IVSVDTLKPLPPNQ GEI VRGPNMM GYFNNP ATK TIDK G
Sbjct: 361 RHTGSAGMLSSGVEAQIVSVDTLKPLPPNQLGEIWVRGPNMMQGYFNNPRATKLTIDKKG 420

Query: 421 WVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEE 480
           WVHTGDLGYFDE+G LYVVDRIKELIKYKGFQVAPAELE LLVSHPEILDAVVIP PD +
Sbjct: 421 WVHTGDLGYFDEDGHLYVVDRIKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPFPDAD 480

Query: 481 AGEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIE 540
           AGEVP+AYVVRS NSSLTE+D+ KFIA QV+ +KRLR+VTFI+SVPKS SGKILRRELI+
Sbjct: 481 AGEVPVAYVVRSPNSSLTEDDVKKFIAGQVASFKRLRKVTFINSVPKSASGKILRRELIQ 540

Query: 541 KVRAKI 544
           KVR+ I
Sbjct: 541 KVRSNI 544

BLAST of CsGy1G013430.2 vs. ExPASy Swiss-Prot
Match: Q9M0X9 (4-coumarate--CoA ligase-like 7 OS=Arabidopsis thaliana OX=3702 GN=4CLL7 PE=1 SV=1)

HSP 1 Score: 750.7 bits (1937), Expect = 1.1e-215
Identity = 374/547 (68.37%), Postives = 463/547 (84.64%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGI+RSLRP LV PKDPN S++SFLFRN  SYP++LAI D+++ +S+++SQL
Sbjct: 1   MEKSGYGRDGIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           K+   R+++G  +LGI KNDVVLIFAPNS QF +CF+ V AIG + TT NP+YTV+E++K
Sbjct: 61  KSAVARLAHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQ----QIPSAIHSPKILCFNDLVNMAGDK 180
           Q++D+ PK++ISV +L+DK+K  ++P VLL      +IP   +S KIL F++++ ++  +
Sbjct: 121 QIKDSNPKIIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNS-KILSFDNVMELS--E 180

Query: 181 SGSEFPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFL 240
             SE+P V +KQSDTAALLYSSGTTG SKGV LTHGNFIA+SLM+TMDQ   GE HGVFL
Sbjct: 181 PVSEYPFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFL 240

Query: 241 NFLPMFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAK 300
            FLPMFHVFGLA ITY+QLQ+GN +VSM +F LE  L  +EK++VT LWVVPPV LAL+K
Sbjct: 241 CFLPMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSK 300

Query: 301 QSLVKKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVG 360
           QS+VKK++LSS+K +GSGAAPLG++LMEEC  NIP+ +++QGYGMTETCG+V++E+P +G
Sbjct: 301 QSIVKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLG 360

Query: 361 KRNSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKH 420
           KRNSGSAG LA GVEA+IVSV+T K  PPNQ GEI VRGPNMM GY NNP+ATK+TIDK 
Sbjct: 361 KRNSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKK 420

Query: 421 GWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDE 480
            WVHTGDLGYF+E+G LYVVDRIKELIKYKGFQVAPAELE LLVSHP+ILDAVVIP PDE
Sbjct: 421 SWVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDE 480

Query: 481 EAGEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELI 540
           EAGEVPIA+VVRS NSS+TE+DI KFIA QV+PYKRLRRV+FIS VPKS +GKILRREL+
Sbjct: 481 EAGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELV 540

Query: 541 EKVRAKI 544
           ++VR+K+
Sbjct: 541 QQVRSKM 544

BLAST of CsGy1G013430.2 vs. ExPASy Swiss-Prot
Match: M4IQS1 (Probable CoA ligase CCL10 OS=Humulus lupulus OX=3486 GN=CCL10 PE=2 SV=1)

HSP 1 Score: 646.4 bits (1666), Expect = 3.0e-184
Identity = 328/544 (60.29%), Postives = 416/544 (76.47%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEK       I+ S RPP+ FP DP LS+ SFLFR+  SYPNR A++DA+S  ++++ +L
Sbjct: 1   MEKCFNPETQIYSSHRPPVNFPTDPKLSLTSFLFRSSASYPNRTALIDADSGQTLTFLKL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           K    ++++ L+QL I+KNDVVLIFAPNS+ F +CF  + A+GAI TTCNP YT +EL+ 
Sbjct: 61  KTQVSKLAHSLVQLNIKKNDVVLIFAPNSIHFPVCFFSIAALGAITTTCNPSYTFTELSN 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           Q +D  P LVI+V ELW+K + LN+P ++L     S + S     F   +    D+  SE
Sbjct: 121 QAKDCNPTLVITVPELWEKARKLNLPAIILPSPSNSKLSSKSEFWFFSDLTRKSDRF-SE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
            PI  V+QSD AALLYSSGTTG SKGV+L+H NFI +SLM+T DQ   G+   + + FLP
Sbjct: 181 LPISDVRQSDVAALLYSSGTTGKSKGVVLSHKNFITTSLMVTSDQDRYGDPQNICMCFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS-L 300
           MFH+FGL+ I Y+QL++GN VVSM KF LE AL AVE Y+VT L+VVPPV++ALAK+S +
Sbjct: 241 MFHIFGLSVIAYSQLRRGNGVVSMGKFELEGALRAVEMYRVTHLFVVPPVMIALAKESVV 300

Query: 301 VKKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRN 360
           V++Y+LSSVK + SGAAPLG+ +ME+CA N+P A +IQGYGMTETCG++++E+   G R 
Sbjct: 301 VRRYDLSSVKEILSGAAPLGKNVMEQCARNVPGAAIIQGYGMTETCGIISIEDSKKGCRF 360

Query: 361 SGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWV 420
           SGS G LA G+E++I+      PLPPNQ GEI +RGPNMM GY NN EATK TID+ GWV
Sbjct: 361 SGSTGMLAPGIESQIMDTKRWNPLPPNQEGEIWLRGPNMMQGYLNNLEATKSTIDEQGWV 420

Query: 421 HTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAG 480
            TGD+GYFDE GQL+VVDR+KELIK  GFQVAPAELEALL+SHPEILDAVVIP PDE+AG
Sbjct: 421 KTGDIGYFDEEGQLFVVDRLKELIKCYGFQVAPAELEALLLSHPEILDAVVIPFPDEKAG 480

Query: 481 EVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKV 540
           EVPIA VVRS NSSL+EED+ +FI  QV+P+K+LRRVTF+SSV KS +GKILRRELI+KV
Sbjct: 481 EVPIANVVRSPNSSLSEEDVQRFIEKQVAPFKKLRRVTFVSSVLKSPAGKILRRELIQKV 540

Query: 541 RAKI 544
           R+KI
Sbjct: 541 RSKI 543

BLAST of CsGy1G013430.2 vs. ExPASy Swiss-Prot
Match: Q0DV32 (4-coumarate--CoA ligase-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL1 PE=2 SV=2)

HSP 1 Score: 580.9 bits (1496), Expect = 1.5e-164
Identity = 302/542 (55.72%), Postives = 393/542 (72.51%), Query Frame = 0

Query: 4   SGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAES-SNSVSYSQLKA 63
           +GYG DG++RSLRPP     DP LS+   L R   + P+ +A+ DA +   ++++++L++
Sbjct: 9   AGYGADGVYRSLRPPAPVASDPGLSLTDLLLRRADACPSAVALADAAAGGRALTFAELRS 68

Query: 64  LAIRVSNGL-IQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTKQ 123
             +  +  L  + G+   D VL+ APN V + +CF  V A+GA+ TT NP YT  E+ KQ
Sbjct: 69  AVLSTAVALSSRAGVRPGDAVLLLAPNCVLYPVCFFAVTALGAVGTTVNPDYTPREIAKQ 128

Query: 124 VRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHS-PKILCFNDLVNMAGDKSGSE 183
           V DA+ KLVI+++ L  K+  L +P +LLD    +A  S P         N+      ++
Sbjct: 129 VSDARAKLVITISALVPKIAGLRLPVILLDDDANAAAASLPPDATVTLYTNLVAGVKEAD 188

Query: 184 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 243
           +    +KQSDTAALLYSSGTTG SKGVILTH NFIA++ M+T DQ    E   VFL FLP
Sbjct: 189 YRRPPIKQSDTAALLYSSGTTGDSKGVILTHRNFIAAARMVTSDQDERREGPNVFLCFLP 248

Query: 244 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 303
           MFH+FGL+ ITYAQL +GN +++M +F++   + AV++++VT L+ VPPV++ALAK    
Sbjct: 249 MFHIFGLSVITYAQLHRGNAIIAMSRFDINSLMEAVQRHRVTHLFCVPPVIIALAKHGKA 308

Query: 304 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGK-RN 363
            KY+LSS+K +GSGAAPLG+++ME  A   P + ++QGYGMTETCG+++LE P  G+ R 
Sbjct: 309 GKYDLSSLKFIGSGAAPLGKDVMEVVAKKFPDSEIVQGYGMTETCGIISLEYPEKGQARE 368

Query: 364 SGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWV 423
            GS GTL SGVEAKIV + TLK LPPNQ GEI VRGPN+M GYFNN +AT+ TI K GW+
Sbjct: 369 FGSTGTLVSGVEAKIVDIKTLKHLPPNQVGEICVRGPNVMQGYFNNVQATEFTI-KQGWL 428

Query: 424 HTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAG 483
           HTGDLGYFD  GQL+VVDR+KELIKYKGFQ+APAELE LL+SHPEILDAVVIP PD +AG
Sbjct: 429 HTGDLGYFDGGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFPDAKAG 488

Query: 484 EVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKV 542
           EVPIAYVVRS +SSLTE D+ KFI  QV+ YKRL+RVTF+ SVPKS SGKILRR+LI +V
Sbjct: 489 EVPIAYVVRSPDSSLTEVDVQKFIEKQVAYYKRLKRVTFVGSVPKSASGKILRRQLIAQV 548

BLAST of CsGy1G013430.2 vs. ExPASy Swiss-Prot
Match: M4IQR7 (Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 2.0e-124
Identity = 238/546 (43.59%), Postives = 362/546 (66.30%), Query Frame = 0

Query: 2   EKSGYGR-DGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 61
           E+SGY + + IF S R P+  P++ ++ + +F+  +  ++  ++A +DA +   +++ QL
Sbjct: 11  ERSGYCKSNSIFYSKREPVQLPQNHSVDVTTFI--SSRAHHGKIAFIDAATGRHLTFPQL 70

Query: 62  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 121
                 V+  L  +GI K DV+L+ +PNS+ F +  + V+++GAI+TT NP+ T  E+ K
Sbjct: 71  WRAVDSVATCLSAMGIRKGDVILLLSPNSIYFPVVCLAVMSLGAIITTTNPLNTPREIAK 130

Query: 122 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 181
           Q+ D+KP L  ++ +L  K+   N+P V++D ++ S++    +   + L  M   +    
Sbjct: 131 QITDSKPVLAFTIPQLVSKIAGSNLPIVIIDDEVKSSLEK-TLNIVSSLGEMMRKEPSPN 190

Query: 182 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEE-HGVFLNFL 241
                V Q DTA LLYSSGTTGASKGV+ +H N IA  ++ T+   F  E+    F+  +
Sbjct: 191 RIGYRVNQEDTATLLYSSGTTGASKGVVSSHKNLIA--MVQTILSRFGTEDGEHTFICTV 250

Query: 242 PMFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS- 301
           PMFH++GLA      L  G+T+V + KF + + L A+EKY+ T L +VPP+++AL K + 
Sbjct: 251 PMFHIYGLAAFAMGLLSSGSTIVILSKFEIHEMLSAIEKYRATYLPLVPPILMALLKNAN 310

Query: 302 -LVKKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGK 361
            +  KY+LSS++ V SG APL +E++E    N P+  ++QGYG+TE+ G+ A  +     
Sbjct: 311 HIRAKYDLSSLQSVLSGGAPLSKEVIEGFVENYPTVSILQGYGLTESTGIGASTDCLQES 370

Query: 362 RNSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHG 421
           R  G+AG L+  +EAKIV+ +T + L  N+ GE+ +RGP +M GYF+N EAT  TID  G
Sbjct: 371 RRYGTAGMLSPSMEAKIVNPETGEALSVNRTGELWLRGPTIMKGYFSNEEATSSTIDSEG 430

Query: 422 WVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEE 481
           W+ TGDL Y DE+G ++VVDR+KELIKYKG+QVAPAELEALL+SHPEI DA VIP PD+E
Sbjct: 431 WLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLSHPEISDAAVIPYPDKE 490

Query: 482 AGEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIE 541
           AG+ P+AYVVR   S+L+E  ++ FIA  V+PYKR+R+V F++S+PK+ SGKILR++LI+
Sbjct: 491 AGQFPMAYVVRKGGSNLSESTVMDFIAKLVAPYKRIRKVAFVASIPKNPSGKILRKDLIK 550

Query: 542 KVRAKI 544
              +K+
Sbjct: 551 LATSKL 551

BLAST of CsGy1G013430.2 vs. NCBI nr
Match: XP_004147290.1 (4-coumarate--CoA ligase-like 7 [Cucumis sativus] >KGN64806.1 hypothetical protein Csa_013302 [Cucumis sativus])

HSP 1 Score: 1064 bits (2752), Expect = 0.0
Identity = 543/543 (100.00%), Postives = 543/543 (100.00%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK
Sbjct: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE
Sbjct: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV
Sbjct: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS
Sbjct: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH
Sbjct: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 543
           AKI
Sbjct: 541 AKI 543

BLAST of CsGy1G013430.2 vs. NCBI nr
Match: XP_008463063.1 (PREDICTED: 4-coumarate--CoA ligase-like 7 [Cucumis melo] >KAA0051818.1 4-coumarate--CoA ligase-like 7 [Cucumis melo var. makuwa] >TYK21426.1 4-coumarate--CoA ligase-like 7 [Cucumis melo var. makuwa])

HSP 1 Score: 1032 bits (2668), Expect = 0.0
Identity = 527/543 (97.05%), Postives = 537/543 (98.90%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSG GRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESS SVSYSQL
Sbjct: 1   MEKSGCGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSYSVSYSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           KALAIR+SNGLIQLGIEKNDVV+IFAPNSVQ +ICFIGVIAIGAIVTTCNPVYTVSELTK
Sbjct: 61  KALAIRLSNGLIQLGIEKNDVVMIFAPNSVQLSICFIGVIAIGAIVTTCNPVYTVSELTK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QVRDAKPKLVISVAELWDKVK+LNIPTVLLDQ+IPSAIHS KILCFNDLVNMAGDKSGSE
Sbjct: 121 QVRDAKPKLVISVAELWDKVKDLNIPTVLLDQKIPSAIHSSKILCFNDLVNMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTH NFIASSLMITMDQTF+GEEHGVFLNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFIASSLMITMDQTFSGEEHGVFLNFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGLACITYAQLQKGNT+VSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV
Sbjct: 241 MFHVFGLACITYAQLQKGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS
Sbjct: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDK+GWVH
Sbjct: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIP PDEEAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDEEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VPIAY+VRSANSSLTEEDILKFIADQVSPYKRLRRVTFIS+VPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYIVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISNVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 543
           AKI
Sbjct: 541 AKI 543

BLAST of CsGy1G013430.2 vs. NCBI nr
Match: XP_038895538.1 (4-coumarate--CoA ligase-like 7 [Benincasa hispida])

HSP 1 Score: 1004 bits (2597), Expect = 0.0
Identity = 510/543 (93.92%), Postives = 529/543 (97.42%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGIFRSLRPPLVFPK PNLSMISFLFRN  SYPNRLAIVDAESS+SVSYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPLVFPKHPNLSMISFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           KA  IRVSNGL+QLGIEKNDVVLIFAPNSVQ++ICF+G+IAIGAIVTTCNPVYTVSELTK
Sbjct: 61  KASVIRVSNGLLQLGIEKNDVVLIFAPNSVQYSICFLGIIAIGAIVTTCNPVYTVSELTK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QVRDAKPKLVISVAELWDKVK+LNIPTVLLDQQIPSAIHS K+LCFNDL+NMAGDKSGS+
Sbjct: 121 QVRDAKPKLVISVAELWDKVKDLNIPTVLLDQQIPSAIHSSKLLCFNDLMNMAGDKSGSD 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FPIV VKQSDTAALLYSSGTTGASKGVILTH NFI SSLMITMDQTF G+E+GV+LNFLP
Sbjct: 181 FPIVEVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITMDQTFRGKEYGVYLNFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS+V
Sbjct: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSS+KRVGSGAAPLG+ELMEEC NNIPSAVV+QGYGMTETCGVVALENPAVGKRNS
Sbjct: 301 KKYNLSSLKRVGSGAAPLGKELMEECGNNIPSAVVLQGYGMTETCGVVALENPAVGKRNS 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNP+ATKQTIDK+GWVH
Sbjct: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPQATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDE GQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIP PD EAGE
Sbjct: 421 TGDLGYFDEYGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDAEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 543
           AKI
Sbjct: 541 AKI 543

BLAST of CsGy1G013430.2 vs. NCBI nr
Match: KAG6607161.1 (4-coumarate--CoA ligase-like 7, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036848.1 4-coumarate--CoA ligase-like 7 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 967 bits (2501), Expect = 0.0
Identity = 483/543 (88.95%), Postives = 520/543 (95.76%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGIFRSLRPP+VFP+DPNLS+ SFLFRN  SYPNRLAIVDAESS+SVSYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           KA AI++SNGL+QLGIEKNDVV+IFAPNSV+F+ICFIG++AIGAIVTTCNPVY VSELTK
Sbjct: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QVRDAKPKL+ISV ELWDKVK+LNIPTVLLDQQIPS I S KILCFNDL+NMAGDKSGSE
Sbjct: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTH NFI SSLMIT+DQ  NG+E+G +LNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGL CIT AQLQ+GNT+VSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS+V
Sbjct: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSS+K +GSGAAPLG+ELM+ECANNIPSAVV+QGYGMTETCGVV+LEN  VGKRN+
Sbjct: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTLASGVEA+IVSVDTLKPLPPNQYGEI VRGPNMMLGY NNP+ATKQTIDK+GWVH
Sbjct: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIP PD+EAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRS NSS+TEED+LKF+ADQVSPYKRLRRVTFI+SVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 543
           AKI
Sbjct: 541 AKI 543

BLAST of CsGy1G013430.2 vs. NCBI nr
Match: XP_022949355.1 (4-coumarate--CoA ligase-like 7 [Cucurbita moschata] >XP_022949356.1 4-coumarate--CoA ligase-like 7 [Cucurbita moschata])

HSP 1 Score: 967 bits (2499), Expect = 0.0
Identity = 482/543 (88.77%), Postives = 520/543 (95.76%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGIFRSLRPP+VFP+DPNLS+ SFLFRN  SYPNRLAIVDAESS+SVSYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           KA AI++SNGL+QLGIEKNDVV+IFAPNSV+F+ICFIG++AIGAIVTTCNPVY VSELTK
Sbjct: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QVRDAKPKL+ISV ELWDKVK+LNIPTVLLDQQIPS I S KILCFNDL+NMAGDKSGSE
Sbjct: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTH NFI SSLMIT+DQ  NG+E+G +LNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGL CIT AQLQ+GNT+VSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS+V
Sbjct: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSS+K +GSGAAPLG+ELM+ECANNIPSAVV+QGYGMTETCGVV+LEN  VGKRN+
Sbjct: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTLASGVEA+IVSVDTLKPLPPNQYGEI VRGPNMMLGY NNP+ATKQTIDK+GWVH
Sbjct: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIP PD+EAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRS NSS+TEED+LKF+ADQVSPYKRLRRVTFI+SVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 543
           AK+
Sbjct: 541 AKL 543

BLAST of CsGy1G013430.2 vs. ExPASy TrEMBL
Match: A0A0A0LXU2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G108800 PE=4 SV=1)

HSP 1 Score: 1064 bits (2752), Expect = 0.0
Identity = 543/543 (100.00%), Postives = 543/543 (100.00%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK
Sbjct: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE
Sbjct: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV
Sbjct: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS
Sbjct: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH
Sbjct: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 543
           AKI
Sbjct: 541 AKI 543

BLAST of CsGy1G013430.2 vs. ExPASy TrEMBL
Match: A0A5D3DCX7 (4-coumarate--CoA ligase-like 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold609G00860 PE=4 SV=1)

HSP 1 Score: 1032 bits (2668), Expect = 0.0
Identity = 527/543 (97.05%), Postives = 537/543 (98.90%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSG GRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESS SVSYSQL
Sbjct: 1   MEKSGCGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSYSVSYSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           KALAIR+SNGLIQLGIEKNDVV+IFAPNSVQ +ICFIGVIAIGAIVTTCNPVYTVSELTK
Sbjct: 61  KALAIRLSNGLIQLGIEKNDVVMIFAPNSVQLSICFIGVIAIGAIVTTCNPVYTVSELTK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QVRDAKPKLVISVAELWDKVK+LNIPTVLLDQ+IPSAIHS KILCFNDLVNMAGDKSGSE
Sbjct: 121 QVRDAKPKLVISVAELWDKVKDLNIPTVLLDQKIPSAIHSSKILCFNDLVNMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTH NFIASSLMITMDQTF+GEEHGVFLNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFIASSLMITMDQTFSGEEHGVFLNFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGLACITYAQLQKGNT+VSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV
Sbjct: 241 MFHVFGLACITYAQLQKGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS
Sbjct: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDK+GWVH
Sbjct: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIP PDEEAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDEEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VPIAY+VRSANSSLTEEDILKFIADQVSPYKRLRRVTFIS+VPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYIVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISNVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 543
           AKI
Sbjct: 541 AKI 543

BLAST of CsGy1G013430.2 vs. ExPASy TrEMBL
Match: A0A1S3CIB0 (4-coumarate--CoA ligase-like 7 OS=Cucumis melo OX=3656 GN=LOC103501298 PE=4 SV=1)

HSP 1 Score: 1032 bits (2668), Expect = 0.0
Identity = 527/543 (97.05%), Postives = 537/543 (98.90%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSG GRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESS SVSYSQL
Sbjct: 1   MEKSGCGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSYSVSYSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           KALAIR+SNGLIQLGIEKNDVV+IFAPNSVQ +ICFIGVIAIGAIVTTCNPVYTVSELTK
Sbjct: 61  KALAIRLSNGLIQLGIEKNDVVMIFAPNSVQLSICFIGVIAIGAIVTTCNPVYTVSELTK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QVRDAKPKLVISVAELWDKVK+LNIPTVLLDQ+IPSAIHS KILCFNDLVNMAGDKSGSE
Sbjct: 121 QVRDAKPKLVISVAELWDKVKDLNIPTVLLDQKIPSAIHSSKILCFNDLVNMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTH NFIASSLMITMDQTF+GEEHGVFLNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFIASSLMITMDQTFSGEEHGVFLNFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGLACITYAQLQKGNT+VSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV
Sbjct: 241 MFHVFGLACITYAQLQKGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS
Sbjct: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDK+GWVH
Sbjct: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIP PDEEAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDEEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VPIAY+VRSANSSLTEEDILKFIADQVSPYKRLRRVTFIS+VPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYIVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISNVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 543
           AKI
Sbjct: 541 AKI 543

BLAST of CsGy1G013430.2 vs. ExPASy TrEMBL
Match: A0A6J1GCI6 (4-coumarate--CoA ligase-like 7 OS=Cucurbita moschata OX=3662 GN=LOC111452734 PE=4 SV=1)

HSP 1 Score: 967 bits (2499), Expect = 0.0
Identity = 482/543 (88.77%), Postives = 520/543 (95.76%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGIFRSLRPP+VFP+DPNLS+ SFLFRN  SYPNRLAIVDAESS+SVSYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           KA AI++SNGL+QLGIEKNDVV+IFAPNSV+F+ICFIG++AIGAIVTTCNPVY VSELTK
Sbjct: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QVRDAKPKL+ISV ELWDKVK+LNIPTVLLDQQIPS I S KILCFNDL+NMAGDKSGSE
Sbjct: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTH NFI SSLMIT+DQ  NG+E+G +LNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGL CIT AQLQ+GNT+VSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS+V
Sbjct: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSS+K +GSGAAPLG+ELM+ECANNIPSAVV+QGYGMTETCGVV+LEN  VGKRN+
Sbjct: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTLASGVEA+IVSVDTLKPLPPNQYGEI VRGPNMMLGY NNP+ATKQTIDK+GWVH
Sbjct: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIP PD+EAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRS NSS+TEED+LKF+ADQVSPYKRLRRVTFI+SVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 543
           AK+
Sbjct: 541 AKL 543

BLAST of CsGy1G013430.2 vs. ExPASy TrEMBL
Match: A0A6J1KDQ6 (4-coumarate--CoA ligase-like 7 OS=Cucurbita maxima OX=3661 GN=LOC111493400 PE=4 SV=1)

HSP 1 Score: 963 bits (2489), Expect = 0.0
Identity = 480/543 (88.40%), Postives = 519/543 (95.58%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGIFRSLRPP+VFP DPNLS+ SFLFRN  SYPNRLAIVDAESS+S SYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPIVFPTDPNLSVASFLFRNSSSYPNRLAIVDAESSDSASYSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           KA AI++SNGL+QLGIEKNDVV+IFAPNSV+F+ICFIG++AIGAIVTTCNPVYTVSEL+K
Sbjct: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYTVSELSK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QVRDAKPKL+ISV ELWDKVK+LNIPTVLLD+QIP  I S KILCFNDL+NMAGDKSGSE
Sbjct: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDKQIPLEIQSSKILCFNDLINMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGV+LTH NFI SSLMIT+DQ  NG+E+G +LNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVVLTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGL CIT AQLQ+GNT+VSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS+V
Sbjct: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSS+K +GSGAAPLG+ELM+ECANNIPSAVV+QGYGMTETCGVV+LEN AVGKRN+
Sbjct: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRAVGKRNT 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTLASGVEA+IVSVDTLKPLPPNQYGEI VRGPNMMLGY NNP+ATKQTIDK+GWVH
Sbjct: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIP PD+EAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRS NSS+TEED+LKF+ADQVSPYKRLRRVTFI+SVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 543
           AKI
Sbjct: 541 AKI 543

BLAST of CsGy1G013430.2 vs. TAIR 10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 750.7 bits (1937), Expect = 8.0e-217
Identity = 374/547 (68.37%), Postives = 463/547 (84.64%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGI+RSLRP LV PKDPN S++SFLFRN  SYP++LAI D+++ +S+++SQL
Sbjct: 1   MEKSGYGRDGIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           K+   R+++G  +LGI KNDVVLIFAPNS QF +CF+ V AIG + TT NP+YTV+E++K
Sbjct: 61  KSAVARLAHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQ----QIPSAIHSPKILCFNDLVNMAGDK 180
           Q++D+ PK++ISV +L+DK+K  ++P VLL      +IP   +S KIL F++++ ++  +
Sbjct: 121 QIKDSNPKIIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNS-KILSFDNVMELS--E 180

Query: 181 SGSEFPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFL 240
             SE+P V +KQSDTAALLYSSGTTG SKGV LTHGNFIA+SLM+TMDQ   GE HGVFL
Sbjct: 181 PVSEYPFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFL 240

Query: 241 NFLPMFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAK 300
            FLPMFHVFGLA ITY+QLQ+GN +VSM +F LE  L  +EK++VT LWVVPPV LAL+K
Sbjct: 241 CFLPMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSK 300

Query: 301 QSLVKKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVG 360
           QS+VKK++LSS+K +GSGAAPLG++LMEEC  NIP+ +++QGYGMTETCG+V++E+P +G
Sbjct: 301 QSIVKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLG 360

Query: 361 KRNSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKH 420
           KRNSGSAG LA GVEA+IVSV+T K  PPNQ GEI VRGPNMM GY NNP+ATK+TIDK 
Sbjct: 361 KRNSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKK 420

Query: 421 GWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDE 480
            WVHTGDLGYF+E+G LYVVDRIKELIKYKGFQVAPAELE LLVSHP+ILDAVVIP PDE
Sbjct: 421 SWVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDE 480

Query: 481 EAGEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELI 540
           EAGEVPIA+VVRS NSS+TE+DI KFIA QV+PYKRLRRV+FIS VPKS +GKILRREL+
Sbjct: 481 EAGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELV 540

Query: 541 EKVRAKI 544
           ++VR+K+
Sbjct: 541 QQVRSKM 544

BLAST of CsGy1G013430.2 vs. TAIR 10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 423.3 bits (1087), Expect = 2.9e-118
Identity = 226/541 (41.77%), Postives = 352/541 (65.06%), Query Frame = 0

Query: 3   KSGY-GRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQLK 62
           +SG+   +  F S R P+  P +P+L + +F+  +  ++  R+A +DA +  ++++++L 
Sbjct: 7   RSGFCNSNSTFYSKRTPIPLPPNPSLDVTTFI--SSQAHRGRIAFIDASTGQNLTFTELW 66

Query: 63  ALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTKQ 122
                V++ L ++GI K  VVL+ +PNS+ F +  + V+++GAI+TT NP+ T +E+ KQ
Sbjct: 67  RAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIAKQ 126

Query: 123 VRDAKPKLVISVAELWDKVKNL--NIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGS 182
           ++D+ P L  + ++L  K+      +P VL+D++   ++   +      LV M   +   
Sbjct: 127 IKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVR-----RLVEMMKKEPSG 186

Query: 183 EFPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIAS-SLMITMDQTFNGEEHGVFLNF 242
                 V Q DTA LLYSSGTTG SKGVI +H N IA    ++    + +GE+   F+  
Sbjct: 187 NRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQR--FICT 246

Query: 243 LPMFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAK-- 302
           +PMFH++GLA      L  G+T++ + KF + + + A+ KY+ T L +VPP+++A+    
Sbjct: 247 VPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGA 306

Query: 303 QSLVKKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVG 362
             +  KY+LSS+  V  G APL +E+ E  A   P+  ++QGYG+TE+ G+ A  +    
Sbjct: 307 DQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVEE 366

Query: 363 KRNSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKH 422
            R  G+AG L++ +E +IV   T + L P Q GE+ ++GP++M GYF+N EAT  T+D  
Sbjct: 367 SRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSE 426

Query: 423 GWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDE 482
           GW+ TGDL Y DE+G ++VVDR+KELIKYKG+QVAPAELEALL++HPEI DA VIP PD+
Sbjct: 427 GWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFPDK 486

Query: 483 EAGEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELI 538
           E G+ P+AYVVR   SSL+E+ I++F+A QV+PYKR+R+V F+SS+PK+ SGKILR++LI
Sbjct: 487 EVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILRKDLI 538

BLAST of CsGy1G013430.2 vs. TAIR 10
Match: AT1G20480.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 416.4 bits (1069), Expect = 3.6e-116
Identity = 238/550 (43.27%), Postives = 339/550 (61.64%), Query Frame = 0

Query: 3   KSGYGRD-GIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNR--LAIVDAESSNSVSYSQ 62
           KSG+     IF S R P+  P +  L + SF    + S P+R     VDA +   +S+ +
Sbjct: 24  KSGFCESTSIFYSKREPMALPPNQFLDVTSF----IASQPHRGKTVFVDAVTGRRLSFPE 83

Query: 63  LKALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELT 122
           L     RV+  L  LG+ K +VV+I +PNS+ F I  + V+++GAI+TT NP+ T  E++
Sbjct: 84  LWLGVERVAGCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSDEIS 143

Query: 123 KQVRDAKPKLVISVAELWDKV---KNLNIPTVLLDQ-QIPSAIHSPKILCFNDLVNMAGD 182
           KQ+ D++P L  +  +L  K+    N N+P VL+D   +PS  +  ++     L  M   
Sbjct: 144 KQIGDSRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLVGRLETMIET 203

Query: 183 KSGSEFPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVF 242
           +         V Q DTAALLYSSGTTG SKGV+L+H N IA  L+      F  E+  + 
Sbjct: 204 EPSESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIA--LVQAYRARFGLEQRTIC 263

Query: 243 LNFLPMFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALA 302
              +PM H+FG        +  G T+V +PKF++ K L AVE ++ + L +VPP+V+A+ 
Sbjct: 264 T--IPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLSLVPPIVVAMV 323

Query: 303 K--QSLVKKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENP 362
                +  KY+LSS+  V +G APL RE+ E+   N P   ++QGYG+TE+  + A    
Sbjct: 324 NGANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTESTAIAASMFN 383

Query: 363 AVGKRNSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTI 422
               +  G++G LA  VE KIV  DT + L  NQ GE+ +R P +M GYF N EAT  TI
Sbjct: 384 KEETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYFKNKEATASTI 443

Query: 423 DKHGWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPC 482
           D  GW+ TGDL Y D +G ++VVDR+KELIK  G+QVAPAELEALL++HPEI DA VIP 
Sbjct: 444 DSEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHPEIADAAVIPI 503

Query: 483 PDEEAGEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRR 542
           PD +AG+ P+AY+VR   S+L+E +I+ F+A QVSPYK++R+VTF++S+PK+ SGKILRR
Sbjct: 504 PDMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYKKIRKVTFLASIPKNPSGKILRR 563

Query: 543 ELIEKVRAKI 544
           EL +   +K+
Sbjct: 564 ELTKLTTSKL 565

BLAST of CsGy1G013430.2 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 401.7 bits (1031), Expect = 9.1e-112
Identity = 228/543 (41.99%), Postives = 337/543 (62.06%), Query Frame = 0

Query: 11  IFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQLKALAIRVSNG 70
           IFRS  P +  P   +L + ++ F  L S  ++  ++   +  S +Y +   +  RV++G
Sbjct: 34  IFRSKLPDIDIPN--HLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASG 93

Query: 71  LIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTKQVRDAKPKLV 130
           L +LGI K DV++I   NS +F   F+G   IGA+ TT NP YT  EL KQ++ +  KL+
Sbjct: 94  LYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLI 153

Query: 131 ISVAELWDKVKNL--NIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSEFPIVGVKQ 190
           I+ ++  DK+KNL  N+  +  D+  P        L F+ L+    D++      V +  
Sbjct: 154 ITHSQYVDKLKNLGENLTLITTDEPTPE-----NCLPFSTLI--TDDETNPFQETVDIGG 213

Query: 191 SDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLN-------FLPM 250
            D AAL +SSGTTG  KGV+LTH      SL+ ++ Q  +G+   ++L         LP+
Sbjct: 214 DDAAALPFSSGTTGLPKGVVLTH-----KSLITSVAQQVDGDNPNLYLKSNDVILCVLPL 273

Query: 251 FHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLVK 310
           FH++ L  +    L+ G TV+ M KF +   L  +++++VT   +VPP+V+ALAK   V 
Sbjct: 274 FHIYSLNSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVN 333

Query: 311 KYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVAL-----ENPAVG 370
            Y+LSSV+ V SGAAPLG+EL +     +P A++ QGYGMTE   V+++     + P   
Sbjct: 334 SYDLSSVRFVLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPT 393

Query: 371 KRNSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKH 430
           K  SGS GT+    E K+V ++T   L  NQ GEI +RG  +M  Y N+PEAT  TID+ 
Sbjct: 394 K--SGSCGTVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEE 453

Query: 431 GWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDE 490
           GW+HTGD+GY DE+ ++++VDR+KE+IK+KGFQV PAELE+LL++H  I DA V+P  DE
Sbjct: 454 GWLHTGDIGYVDEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDE 513

Query: 491 EAGEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELI 540
            AGEVP+A+VVRS  + +TEED+ +++A QV  YKRL +V F++S+PKS SGKILR++L 
Sbjct: 514 VAGEVPVAFVVRSNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLK 560

BLAST of CsGy1G013430.2 vs. TAIR 10
Match: AT1G20500.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 400.2 bits (1027), Expect = 2.6e-111
Identity = 232/545 (42.57%), Postives = 342/545 (62.75%), Query Frame = 0

Query: 3   KSGYGR-DGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQLK 62
           +SG+ + +  F S R PL  P  PNLS     F +   +  + A +DA +   +++S L 
Sbjct: 13  RSGFCKSNSTFYSKRQPLSLP--PNLSRDVTTFISSQPHRGKTAFIDAATGQCLTFSDLW 72

Query: 63  ALAIRVSNGLI-QLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 122
               RV++ L  ++GI + DVVLI +PNS+   +  + V+++GA+ TT N + T  E++K
Sbjct: 73  RAVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTLNTSGEISK 132

Query: 123 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 182
           Q+ D+ P LV +  +L  K+  + I  VL D ++   + S  I     L  M   +   +
Sbjct: 133 QIADSNPTLVFTTRQLAPKLP-VAISVVLTDDEVYQELTS-AIRVVGILSEMVKKEPSGQ 192

Query: 183 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 242
                V Q DTA +LYSSGTTG SKGVI +H N  A       D   N +   +F+  +P
Sbjct: 193 RVRDRVNQDDTAMMLYSSGTTGPSKGVISSHRNLTAHVARFISD---NLKRDDIFICTVP 252

Query: 243 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS-L 302
           MFH +GL       +  G+TVV + +F L   + AVEK++ T L + PPV++A+   + L
Sbjct: 253 MFHTYGLLTFAMGTVALGSTVVILRRFQLHDMMDAVEKHRATALALAPPVLVAMINDADL 312

Query: 303 VK-KYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKR 362
           +K KY+LSS+K V  G APL +E+ E      P+  ++QGY +TE+ G  A  N A   R
Sbjct: 313 IKAKYDLSSLKTVRCGGAPLSKEVTEGFLEKYPTVDILQGYALTESNGGGAFTNSAEESR 372

Query: 363 NSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGW 422
             G+AGTL S VEA+IV  +T + +  NQ GE+ ++GP++  GYF N EAT +TI+  GW
Sbjct: 373 RYGTAGTLTSDVEARIVDPNTGRFMGINQTGELWLKGPSISKGYFKNQEATNETINLEGW 432

Query: 423 VHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEA 482
           + TGDL Y DE+G L+VVDR+KELIKYKG+QV PAELEALL++HP+ILDA VIP PD+EA
Sbjct: 433 LKTGDLCYIDEDGFLFVVDRLKELIKYKGYQVPPAELEALLITHPDILDAAVIPFPDKEA 492

Query: 483 GEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEK 542
           G+ P+AYVVR   S+L+E+ ++ FI+ QV+PYK++R+V+FI+S+PK+ SGK LR++LI+ 
Sbjct: 493 GQYPMAYVVRKHESNLSEKQVIDFISKQVAPYKKIRKVSFINSIPKTASGKTLRKDLIKL 550

Query: 543 VRAKI 544
             +K+
Sbjct: 553 ATSKL 550

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
M4IRL64.7e-22270.33Probable CoA ligase CCL7 OS=Humulus lupulus OX=3486 GN=CCL7 PE=2 SV=1[more]
Q9M0X91.1e-21568.374-coumarate--CoA ligase-like 7 OS=Arabidopsis thaliana OX=3702 GN=4CLL7 PE=1 SV=... [more]
M4IQS13.0e-18460.29Probable CoA ligase CCL10 OS=Humulus lupulus OX=3486 GN=CCL10 PE=2 SV=1[more]
Q0DV321.5e-16455.724-coumarate--CoA ligase-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL1... [more]
M4IQR72.0e-12443.59Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_004147290.10.0100.004-coumarate--CoA ligase-like 7 [Cucumis sativus] >KGN64806.1 hypothetical protei... [more]
XP_008463063.10.097.05PREDICTED: 4-coumarate--CoA ligase-like 7 [Cucumis melo] >KAA0051818.1 4-coumara... [more]
XP_038895538.10.093.924-coumarate--CoA ligase-like 7 [Benincasa hispida][more]
KAG6607161.10.088.954-coumarate--CoA ligase-like 7, partial [Cucurbita argyrosperma subsp. sororia] ... [more]
XP_022949355.10.088.774-coumarate--CoA ligase-like 7 [Cucurbita moschata] >XP_022949356.1 4-coumarate-... [more]
Match NameE-valueIdentityDescription
A0A0A0LXU20.0100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G108800 PE=4 SV=1[more]
A0A5D3DCX70.097.054-coumarate--CoA ligase-like 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3CIB00.097.054-coumarate--CoA ligase-like 7 OS=Cucumis melo OX=3656 GN=LOC103501298 PE=4 SV=1[more]
A0A6J1GCI60.088.774-coumarate--CoA ligase-like 7 OS=Cucurbita moschata OX=3662 GN=LOC111452734 PE=... [more]
A0A6J1KDQ60.088.404-coumarate--CoA ligase-like 7 OS=Cucurbita maxima OX=3661 GN=LOC111493400 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT4G05160.18.0e-21768.37AMP-dependent synthetase and ligase family protein [more]
AT1G20510.12.9e-11841.77OPC-8:0 CoA ligase1 [more]
AT1G20480.13.6e-11643.27AMP-dependent synthetase and ligase family protein [more]
AT1G65060.19.1e-11241.994-coumarate:CoA ligase 3 [more]
AT1G20500.12.6e-11142.57AMP-dependent synthetase and ligase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 38..445
e-value: 8.0E-106
score: 354.2
NoneNo IPR availableGENE3D3.30.300.30coord: 441..542
e-value: 4.8E-31
score: 109.5
NoneNo IPR availablePANTHERPTHR24096:SF3574-COUMARATE:COA LIGASE-LIKE PROTEINcoord: 4..542
NoneNo IPR availablePANTHERPTHR24096LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 4..542
NoneNo IPR availableCDDcd059044CLcoord: 21..535
e-value: 0.0
score: 735.581
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 17..541
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 454..529
e-value: 7.3E-15
score: 55.7
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 10..440
e-value: 1.2E-131
score: 441.4
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 194..205

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CsGy1G013430CsGy1G013430gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CsGy1G013430.2.exon6CsGy1G013430.2.exon6exon
CsGy1G013430.2.exon5CsGy1G013430.2.exon5exon
CsGy1G013430.2.exon4CsGy1G013430.2.exon4exon
CsGy1G013430.2.exon3CsGy1G013430.2.exon3exon
CsGy1G013430.2.exon2CsGy1G013430.2.exon2exon
CsGy1G013430.2.exon1CsGy1G013430.2.exon1exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CsGy1G013430.2.utr3p1CsGy1G013430.2.utr3p1three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.CsGy1G013430.2cds.CsGy1G013430.2_6CDS
cds.CsGy1G013430.2cds.CsGy1G013430.2_5CDS
cds.CsGy1G013430.2cds.CsGy1G013430.2_4CDS
cds.CsGy1G013430.2cds.CsGy1G013430.2_3CDS
cds.CsGy1G013430.2cds.CsGy1G013430.2_2CDS
cds.CsGy1G013430.2cds.CsGy1G013430.2CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CsGy1G013430.2.utr5p1CsGy1G013430.2.utr5p1five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CsGy1G013430.2CsGy1G013430.2-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016405 CoA-ligase activity