Cp4.1LG13g07980.1 (mRNA) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG13g07980.1 vs. ExPASy Swiss-Prot
Match: Q9UT45 (Primary septum endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng1 PE=3 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 1.7e-33
Identity = 164/678 (24.19%), Postives = 279/678 (41.15%), Query Frame = 0

Query: 81  SPELLSAPLPTNSFFQNFTLNNGDQPEFIHPYLI----KSSLSSISVSY----------- 140
           S + LS+P+ TN FF N  L +     +  P+        ++  I +++           
Sbjct: 74  STDSLSSPIETNKFFDNNLLGSRTNFMYADPFRYWWQSSDTMGGICIAHTDDNQRVMDTD 133

Query: 141 ---PSISSKSASICQVFI--PDLTISPSDKIDPLPQKSHVISSFNDLNVTLDIPSSNLRF 200
              PS   +   IC +      +T +    +D + Q S           T    SS+++ 
Sbjct: 134 DTIPSYYYEPIGICSLGFGASGITSNTDPIVDEIDQMS--------ARFTFSWDSSSMQL 193

Query: 201 YLVRGSPFLTFTVSKGVAFSIST---IHEVISFSFNNALTKYTIKLKNNQTWIIY---SS 260
            L  G    T   +  +    S+   I++ +     +A+ KY + + +N  W+IY    S
Sbjct: 194 TLTEGMAVTTAVYTNAIPQIFSSTLYINDFVEVPGTSAVQKYRVTMSDNHVWLIYIYGDS 253

Query: 261 LPINLTHSLSVITSGGYAGIIRIAALPNNDPECEPILDRFSSCY----PISGEVRFTKPF 320
           L +  + S  ++ S  + G I+IA +P  D   E + D ++  Y     ISG V     +
Sbjct: 254 LTLTESTSQMLVGSNTFNGYIQIAKIPLGDGTAEALYDTYAGVYITGISISGYVEDAVGY 313

Query: 321 CLEYKWETKGWGD------LLMLAHPLHLRLLAGTDNDVVILDKFKYRSIDGELVGVVGS 380
              Y ++    GD        +L H +    ++GT    ++L       + G++    G+
Sbjct: 314 ---YSFDFTTAGDTSVEPLFFLLPHQVD-TAVSGTKVTSIVLASL----VSGDMNAAAGN 373

Query: 381 SWALK---PEPI-LVSWH---SMKGVEEESFTDIISALDKDIESLNSTTMTTKSPYSYGK 440
           S       P+ I  + W       G  EE+   I      ++    S      S Y  GK
Sbjct: 374 SITFAEAIPQDIGFLPWSPTGGQIGYSEEALEIIAEVAGTELGEDFSANSNLNSMYYSGK 433

Query: 441 LIARAARLAVIAEEVRYLQVLPD--IRKFLMGAIEPWLNGTLEGNGFVYDEKWGGIISKE 500
           ++A+ A L V   ++   +   +  I+K L  A   +++   +     YD  W G++S  
Sbjct: 434 VLAKYAMLCVTINDILGDETSSEQCIQK-LEAAFARFVDNQ-QIYPLTYDNTWKGVVSVA 493

Query: 501 G-SFHSGADFGFGIYNNHHHTLGYFFYAIAVLVKIDPAWGRKYSPQ--VYALMADIMNLS 560
           G S  S ADFG   YN+HH   GYF +  AV+  IDP W    + +  V  L+ D+ N S
Sbjct: 494 GLSGDSLADFGNSYYNDHHFHYGYFVFTAAVIGHIDPDWINTGNNKEWVNFLVRDVANPS 553

Query: 561 KQSNSKFPRLRCFDAYKLHSWGTGLAEFMDGRSQESISEAVNAYYSAALVGLAYGDADLV 620
             ++  FP+ R  D Y  H W +GL E  DG+ +ES SE  N ++   L G   GD+D+ 
Sbjct: 554 -SNDPYFPKHRMIDIYHGHGWASGLFESNDGKDEESTSEDYNFFFGMKLWGQVIGDSDME 613

Query: 621 SIGATLAALEIKAGQMWWQVREGQALYEEEFVKENRLVGVLWSNKRDSGLWFGPGEWKEC 680
                +  +E  A   +    +G    +   ++ N + G+ + NK     +FG     E 
Sbjct: 614 DRANIILGIERNALNKYMLYADGNV--QPTSMQPNYVAGITFMNKITHTTYFGTN--IEY 673

Query: 681 RLGIQVLPMLPITELLLSDVGFVREAVNWALPSLGREGVGEAWKGFVYGMESVYDKAGAL 707
             GI +LP+ PI+  +     FV    N  L S+  + V   W+  +Y   ++ +   + 
Sbjct: 674 IQGIHMLPITPISAFIRGP-SFVLAEWNALLASV-IDYVDSGWRSLLYANLAIAEPEESY 726

BLAST of Cp4.1LG13g07980.1 vs. ExPASy Swiss-Prot
Match: Q09850 (Ascus wall endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng2 PE=1 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 4.6e-31
Identity = 166/676 (24.56%), Postives = 281/676 (41.57%), Query Frame = 0

Query: 66  PSTHSTVLPDPSLFFSPELLSAPLPTNSFFQNFTLNNGDQPEFIHPYLIK----SSLSSI 125
           P   S   P P L  +  L   P+ TN F+ N  +     P + HPY +     SS   +
Sbjct: 14  PVFPSRAHPSPPLGLTSNLF--PIQTNKFYGNLYIGTRHNPSWSHPYSVTWLNGSSYYGL 73

Query: 126 SVSYPSISSKSASICQVFIPDLTISPSD-KIDPLPQKSHVISSFNDLNVTLDIPSSNLRF 185
           ++S+   S +      VF PD    P     +P    S +IS+    +  L     +  F
Sbjct: 74  AISHIDDSQR------VFGPDPESVPCQYYFNPAGLYSIIISAREFASGNLLSLDQSRHF 133

Query: 186 YL-------VRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALT-------------KYT 245
            +         GS  +   +  G+ F +S  +  ++  FN+++              KY 
Sbjct: 134 SIQATLSATTSGSGTIILPIVAGMGF-VSGYYTNLTPVFNSSILFSSITKINFSRGYKYR 193

Query: 246 IKLKNNQTWIIY-------SSLPINLTHSLSVITSGGYAGIIRIAALPN---NDPECEPI 305
           I+L + + W +Y       S+  + L  +  + TS  + G+I+I  +PN   N+   + I
Sbjct: 194 IQLTDGKIWFLYAFPTSSSSTFNLTLASNSQLTTSTKFTGLIQICKVPNESVNNSYPDTI 253

Query: 306 LDRFSSCY----PISGEVRFTKPFCLEY--KWETKGWGDL--LMLAHPLHLRLLAGTDND 365
            D  +  Y     +S +V  T     EY  ++ T G+ +L  LM A P H++   G+D  
Sbjct: 254 YDASAGVYTTSISLSAQVSGTTG---EYWFRFATAGYTNLNPLMFALPHHMQSF-GSDTQ 313

Query: 366 VVILDKFKYRSIDGELVGVVGSSWAL--KPEP-----ILVSWHSMKGVEEESFTDII--- 425
                     +  G +      +W L  K  P     + + W+        +    I   
Sbjct: 314 AYKTGLGLASTTMGIMFAYATKTWHLIEKNLPTQVGFLPIPWNGGSNTYSPTALAAIRAA 373

Query: 426 SALDKDIESLNSTTMTTKSPYSYGKLIARAARLAVIAEEVRYLQVLPDIRKFLMGAIEPW 485
            A D + + +N++ +   S Y+ GK++A  A++ ++A  +     L +     +      
Sbjct: 374 CATDINFDVVNASNL--DSMYTSGKIVAMYAQVCLVASRILGDSTLTNTGLTKLKQAMAR 433

Query: 486 LNGTLEGNGFVYDEKWGGIISKEGSFHSGADFGFGIYNNHHHTLGYFFYAIAVLVKIDPA 545
                +    VYD  + GIIS  G     AD+G   YN+HH   GY  YA AV+  +DP+
Sbjct: 434 FTTNTQMYPLVYDTTYKGIISTAGYSSPLADYGNTYYNDHHFHWGYHIYACAVIGLLDPS 493

Query: 546 W-GRKYSPQVYALMADIMNLSKQSNSKFPRLRCFDAYKLHSWGTGLAEFMDGRSQESISE 605
           W        V AL+ D  N S +S++ F   R FD +  HSW TG+ E  DG+ +ES SE
Sbjct: 494 WLVNDNIRYVNALLRDSANPS-ESDTYFAMFRNFDWFVGHSWATGIFESGDGKDEESTSE 553

Query: 606 AVNAYYSAALVGLAYGDADLVSIGATLAALEIKAGQMWWQVREGQALYEEEFVKENRLVG 665
             N  Y+  L G+   D  L++  A L    +K     +         +   +  N + G
Sbjct: 554 DFNFLYATKLWGMVRNDTVLIN-RANLMLAVLKNSLNTYIYMTPTTSVQPSQILGNYVTG 613

Query: 666 VLWSNKRDSGLWFGPGEWKECRLGIQVLPMLPITELLLSDVGFVREAVNWALPSLGREGV 688
           + + NK D   +F   E+  C+ GI ++P  PI+  L S   +V++  N  +  +     
Sbjct: 614 ITFMNKVDYATYFSAEEY--CKQGIHMIPTTPISGYLRSP-SYVQQDWNAKIAPI-INTF 668

BLAST of Cp4.1LG13g07980.1 vs. ExPASy Swiss-Prot
Match: D4AZ24 (Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_01444 PE=1 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 1.4e-19
Identity = 191/779 (24.52%), Postives = 316/779 (40.56%), Query Frame = 0

Query: 16  KRGSPPPRLPPPP-------------------PTSPPPPPPPPPPPPEPPQSPPPEPSQK 75
           KR   P R PP P                   PT+  P    P  P  PP +   +   +
Sbjct: 136 KRSPAPQRHPPAPTKATAGYQFTNCTSTSNPGPTATSPTSGIPSQPSAPPATMAGQDIFQ 195

Query: 76  PSEPTPFLFPSTHSTVLPDPSLFFSPELLSAPLPTNSFFQNFTLNNGDQPEFIHPYLI-- 135
           P    P   P+   +    P      E  + P+ TN F+ NF L N     F HPY +  
Sbjct: 196 PIAKDP--IPANIKSRDDHPVKANHIENPTGPISTNKFYANFFLGNQTSTTFTHPYTMIW 255

Query: 136 -----KSSLSSISVSYPSISSKSASICQVFIPDLTISPSDKIDPLPQKSHVISSF---ND 195
                 +S   +++S+   S ++       +P   +     I+P+  KS V+S+      
Sbjct: 256 AKGDKNASSFGMAISHVEPSQRATGEPNNKLPGNPV--RYYINPVGIKSLVLSASELKES 315

Query: 196 LNVTLDIPSSNLRFYLVR----GSPFLTFTVSKGVAFSISTIHEVISFSFNNA------- 255
             +++  P +     ++R     S  +TF + +G+ F I+ I+  +  +  +A       
Sbjct: 316 TTMSVAKPQAFSAQAILRPTGGSSESITFPLVQGMGF-ITAIYNNLQPAIQSAVLFRKVE 375

Query: 256 --------LTKYTIKLKNNQTWIIY------SSLPINLTHSLSVITSGGYAGIIRIAALP 315
                   + KY I L++++ W++Y      +   + L  +  +    G+ G+I++A  P
Sbjct: 376 PAGSPQGGIFKYKITLEDDKNWLLYVIPENGADPKLKLEGNKLISGPTGFKGVIQVAKNP 435

Query: 316 NNDPECEPILDRFSSCY----PISGEVRFTKPFCLEYKWETKGWG-DLLMLAHPLHLRLL 375
           + + E E I D+ +  Y     ISG V        ++ +E  G G  L+M A P H+   
Sbjct: 436 SAE-EGEGIYDKSAGSYATDIKISGSVGTDGTGTYKFSFEKAGKGAPLVMYALPHHVESF 495

Query: 376 AGTDNDVVILDKFKYRSIDGELVGVVGSSWALKPEPILVS-----W---HSMKGVEEESF 435
                +     K    +  G     VG SW +    + +S     W    S +    E  
Sbjct: 496 DDATKNTKKNMKLS-TTTKGMATACVGDSWTMVEGNLPLSMDFAPWKPGSSSQVTLSEGA 555

Query: 436 TDIISA-----LDKDIE---SLNSTTMTTKSPYSYGKLIARAARLAVIAEEVRYLQVLPD 495
            + I A     L +D+E   +LNS   + K    +   I     L  + ++      L  
Sbjct: 556 KNAIKAVAGNELSQDMELQTNLNSMYFSGKGLNKFAGAIYTVQEL--VGDKAAASGPLNS 615

Query: 496 IRKFLMGAIEPWLNGTLEGNGFVYDEKWGGIISKEGSFH---SGADFGFGIYNNHHHTLG 555
           +++     ++       +    VYD  W G++S  G++    +G DFG  +YN+HH   G
Sbjct: 616 LKESFKRFVD-----NKQQIPLVYDNVWKGVVS-SGTYEKGDTGLDFGNTLYNDHHFHYG 675

Query: 556 YFFYAIAVLVKIDPAWGRKYSPQVYALMADIMNLSKQSNSKFPRLRCFDAYKLHSWGTGL 615
           YF    A+L K+DPAW       V  L+ D  N S  ++  FP  R FD Y  HSW  GL
Sbjct: 676 YFILTAAILGKLDPAWLDANKAYVNMLVRDSGN-SVDNDEHFPFSRAFDWYHGHSWAKGL 735

Query: 616 AEFMDGRSQESISEAVNAYYSAALVGLAYGDADLVSIGATLAALEIKAGQMWWQVREGQA 675
            E  DG+ QES SE     Y+  + G   GD  + + G  +  +  +    ++ ++    
Sbjct: 736 FESSDGKDQESTSEDTMYAYAIKMWGKTSGDKSMEARGNLMLGILARTLNNYFLMKNDNV 795

Query: 676 LYEEEFVKENRLVGVLWSNKRDSGLWFGPGEWKECRLGIQVLPMLPITELLLSDVGFVRE 707
              + F+  N++ G+L+ NK D   +FG     E   GI +LP+LP +    S   FV+E
Sbjct: 796 NQPKNFI-GNKVTGILFENKIDHTTYFGAN--LEYIQGIHMLPLLPNSAYTRS-AEFVKE 855

BLAST of Cp4.1LG13g07980.1 vs. ExPASy Swiss-Prot
Match: Q12168 (Endo-1,3(4)-beta-glucanase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ACF2 PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 9.1e-19
Identity = 186/786 (23.66%), Postives = 320/786 (40.71%), Query Frame = 0

Query: 16  KRGSPPPRLPPPPPTSPPPPPPPPPPPPEPPQSPPPEPSQKPSE--------PTPFLFPS 75
           ++  PPP    P  T+   PPP PP     P     E S KP E         TP     
Sbjct: 5   RQAIPPPVPNRPGGTTNRGPPPLPPRANVQPPVCSSENSSKPRENRVAGESLRTPSSSNP 64

Query: 76  THSTVLPDPSLFFSPELLSA-------------------------PLPTNSFFQNFTLNN 135
              + +   ++F SP L +                          PL TN F+ N  L++
Sbjct: 65  LADSQVNSDNIFQSPVLSNLKAPPSVFNKVQHPVPKPNIDDQSVDPLETNKFYTNMLLDD 124

Query: 136 GDQPEFIHPYLIKSS----LSSISVSYPSISSKSASICQVFIPDLTISPSDKIDPLPQKS 195
             QP + HPY I  S    L  ++ ++ +++S+         P    +P++ I     K+
Sbjct: 125 NTQPIWTHPYSIWFSRDPELFGLAANH-TLASQRVFDTTTNPPRFYFNPTN-IKSFVFKA 184

Query: 196 HVISSFNDLNVTL-DIPSSNLRFYL-VRGSPFLTFTVSKGVAFSISTIHEV-------IS 255
               S ND+ +   D+   ++   + +  S F+ F + +G+ F  +  H++       + 
Sbjct: 185 REFVSSNDIKLEFRDMKHMSMCLLMSLSSSQFIEFPLVQGMGFVTAIYHDLGFELRSAVG 244

Query: 256 F------SFNNALTKYTIKLKNNQTWIIYSSLP---------INLTHSLSVITSGGYAGI 315
           F      S N    KY I+L+NN+ WI+Y + P         I+L  S ++I+S    G+
Sbjct: 245 FRSLERISVNERYGKYNIQLENNRNWILYLTSPDYSFPQDFQISLLDSNTIISSHKINGL 304

Query: 316 I-RIAALPNNDPECEPILDRFSSCYPI----SGEVRFTKPFCLEYKWETKGW---GDLLM 375
           I +++A      +  P +D  + CYP+    SG+          + +   G+   G  LM
Sbjct: 305 ICQLSA------DSVPSIDMAAGCYPVYCDLSGQTVDEHFTNYRFNYTVAGYSQSGTTLM 364

Query: 376 LAHPLHLRLLAGTDNDVVILDKFKYRSIDGELVGVVGSSWALKP--------EPILVSWH 435
            A P H         +  I       ++ G + G + +S+ ++         EP+ +S +
Sbjct: 365 YALPHHKAAFTPEMQEREIASSLD-STVKGLMTGYLTNSFDMQVQVPQELGFEPVALSLN 424

Query: 436 SMKGVEEESFTDIISALDKDIE-SLNSTTMTTKSPYSYGKLIARAARLAVIAEEVRYLQV 495
                 +E  + I  A  ++++ S         S Y  GK++A+ A +  +   + + + 
Sbjct: 425 KKADYSQEKLSKIREAAVQEVQLSDPQQESNIDSMYFSGKILAKYAWILYVTHYILHDEN 484

Query: 496 L-PDIRKFLMGAIEPWLNGTLEGNGFVYDEKWGGIISKEGSFHSGADFGFGIYNNHHHTL 555
           L  ++   L  A+E +++   +     YD  W GIIS   S  S  DFG   YN+HH   
Sbjct: 485 LTKELLSKLTIAMERFISNQ-QVLPLNYDVSWKGIIS---SGSSSQDFGNSYYNDHHFHY 544

Query: 556 GYFFYAIAVLVKIDP--------AWGRKYSPQVYALMADIMNLSKQSNSKFPRLRCFDAY 615
            Y     A++  +D         +W       V  L+ D   +    +  FP+ R FD +
Sbjct: 545 SYHVITAAIISLVDSDLSGVTNNSWLENNRDWVECLIRDYSGVD-NDDPYFPQFRSFDWF 604

Query: 616 KLHSWGTGLAEFMDGRSQESISEAVNAYYSAALVGLAYGDADLVSIGATLAALEIKAGQM 675
             HSW  GL    DG+ +ES SE VN+ Y+  L GL  G++ L  I      +     Q 
Sbjct: 605 NGHSWAKGLFPSGDGKDEESTSEDVNSCYAIKLWGLVTGNSKLTDIANLQLGIMRNVFQS 664

Query: 676 WWQVREGQALYEEEFVKENRLVGVLWSNKRDSGLWFGPGEWKECRLGIQVLPMLPITEL- 709
           ++       +  +EF+  N++ G+L+ NK D   +FG          I ++  +PIT   
Sbjct: 665 YFLYESNNTVQPKEFI-GNKVSGILFENKIDHATYFGMEPQY-----IHMIHAIPITSAS 724

BLAST of Cp4.1LG13g07980.1 vs. NCBI nr
Match: XP_023550602.1 (probable endo-1,3(4)-beta-glucanase ARB_01444 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1447 bits (3747), Expect = 0.0
Identity = 733/733 (100.00%), Postives = 733/733 (100.00%), Query Frame = 0

Query: 1   MFKKIERELRRAIKGKRGSPPPRLPPPPPTSPPPPPPPPPPPPEPPQSPPPEPSQKPSEP 60
           MFKKIERELRRAIKGKRGSPPPRLPPPPPTSPPPPPPPPPPPPEPPQSPPPEPSQKPSEP
Sbjct: 9   MFKKIERELRRAIKGKRGSPPPRLPPPPPTSPPPPPPPPPPPPEPPQSPPPEPSQKPSEP 68

Query: 61  TPFLFPSTHSTVLPDPSLFFSPELLSAPLPTNSFFQNFTLNNGDQPEFIHPYLIKSSLSS 120
           TPFLFPSTHSTVLPDPSLFFSPELLSAPLPTNSFFQNFTLNNGDQPEFIHPYLIKSSLSS
Sbjct: 69  TPFLFPSTHSTVLPDPSLFFSPELLSAPLPTNSFFQNFTLNNGDQPEFIHPYLIKSSLSS 128

Query: 121 ISVSYPSISSKSASICQVFIPDLTISPSDKIDPLPQKSHVISSFNDLNVTLDIPSSNLRF 180
           ISVSYPSISSKSASICQVFIPDLTISPSDKIDPLPQKSHVISSFNDLNVTLDIPSSNLRF
Sbjct: 129 ISVSYPSISSKSASICQVFIPDLTISPSDKIDPLPQKSHVISSFNDLNVTLDIPSSNLRF 188

Query: 181 YLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWIIYSSLPINLT 240
           YLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWIIYSSLPINLT
Sbjct: 189 YLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWIIYSSLPINLT 248

Query: 241 HSLSVITSGGYAGIIRIAALPNNDPECEPILDRFSSCYPISGEVRFTKPFCLEYKWETKG 300
           HSLSVITSGGYAGIIRIAALPNNDPECEPILDRFSSCYPISGEVRFTKPFCLEYKWETKG
Sbjct: 249 HSLSVITSGGYAGIIRIAALPNNDPECEPILDRFSSCYPISGEVRFTKPFCLEYKWETKG 308

Query: 301 WGDLLMLAHPLHLRLLAGTDNDVVILDKFKYRSIDGELVGVVGSSWALKPEPILVSWHSM 360
           WGDLLMLAHPLHLRLLAGTDNDVVILDKFKYRSIDGELVGVVGSSWALKPEPILVSWHSM
Sbjct: 309 WGDLLMLAHPLHLRLLAGTDNDVVILDKFKYRSIDGELVGVVGSSWALKPEPILVSWHSM 368

Query: 361 KGVEEESFTDIISALDKDIESLNSTTMTTKSPYSYGKLIARAARLAVIAEEVRYLQVLPD 420
           KGVEEESFTDIISALDKDIESLNSTTMTTKSPYSYGKLIARAARLAVIAEEVRYLQVLPD
Sbjct: 369 KGVEEESFTDIISALDKDIESLNSTTMTTKSPYSYGKLIARAARLAVIAEEVRYLQVLPD 428

Query: 421 IRKFLMGAIEPWLNGTLEGNGFVYDEKWGGIISKEGSFHSGADFGFGIYNNHHHTLGYFF 480
           IRKFLMGAIEPWLNGTLEGNGFVYDEKWGGIISKEGSFHSGADFGFGIYNNHHHTLGYFF
Sbjct: 429 IRKFLMGAIEPWLNGTLEGNGFVYDEKWGGIISKEGSFHSGADFGFGIYNNHHHTLGYFF 488

Query: 481 YAIAVLVKIDPAWGRKYSPQVYALMADIMNLSKQSNSKFPRLRCFDAYKLHSWGTGLAEF 540
           YAIAVLVKIDPAWGRKYSPQVYALMADIMNLSKQSNSKFPRLRCFDAYKLHSWGTGLAEF
Sbjct: 489 YAIAVLVKIDPAWGRKYSPQVYALMADIMNLSKQSNSKFPRLRCFDAYKLHSWGTGLAEF 548

Query: 541 MDGRSQESISEAVNAYYSAALVGLAYGDADLVSIGATLAALEIKAGQMWWQVREGQALYE 600
           MDGRSQESISEAVNAYYSAALVGLAYGDADLVSIGATLAALEIKAGQMWWQVREGQALYE
Sbjct: 549 MDGRSQESISEAVNAYYSAALVGLAYGDADLVSIGATLAALEIKAGQMWWQVREGQALYE 608

Query: 601 EEFVKENRLVGVLWSNKRDSGLWFGPGEWKECRLGIQVLPMLPITELLLSDVGFVREAVN 660
           EEFVKENRLVGVLWSNKRDSGLWFGPGEWKECRLGIQVLPMLPITELLLSDVGFVREAVN
Sbjct: 609 EEFVKENRLVGVLWSNKRDSGLWFGPGEWKECRLGIQVLPMLPITELLLSDVGFVREAVN 668

Query: 661 WALPSLGREGVGEAWKGFVYGMESVYDKAGALQKIRNLKAFDDGNSLTNLLWWVHSRPKE 720
           WALPSLGREGVGEAWKGFVYGMESVYDKAGALQKIRNLKAFDDGNSLTNLLWWVHSRPKE
Sbjct: 669 WALPSLGREGVGEAWKGFVYGMESVYDKAGALQKIRNLKAFDDGNSLTNLLWWVHSRPKE 728

Query: 721 GEANISCTKVQLL 733
           GEANISCTKVQLL
Sbjct: 729 GEANISCTKVQLL 741

BLAST of Cp4.1LG13g07980.1 vs. NCBI nr
Match: XP_022939114.1 (endo-1,3(4)-beta-glucanase 2-like [Cucurbita moschata])

HSP 1 Score: 1419 bits (3673), Expect = 0.0
Identity = 719/734 (97.96%), Postives = 724/734 (98.64%), Query Frame = 0

Query: 1   MFKKIERELRRAIKGKRGSPPPRLPPPPPTSPPPPPPPPPPPP-EPPQSPPPEPSQKPSE 60
           MFKKIERELRR  KGKR SPPPRLPPPPPTSPPPPPPPPPPPP EPPQSPPPEPSQ PSE
Sbjct: 9   MFKKIERELRRVTKGKRSSPPPRLPPPPPTSPPPPPPPPPPPPPEPPQSPPPEPSQNPSE 68

Query: 61  PTPFLFPSTHSTVLPDPSLFFSPELLSAPLPTNSFFQNFTLNNGDQPEFIHPYLIKSSLS 120
           PTPFLFPSTHSTVLPDPSLFFSPELLSAPLPTNSFFQNFTLNNGDQPEFIHPYLIKSSLS
Sbjct: 69  PTPFLFPSTHSTVLPDPSLFFSPELLSAPLPTNSFFQNFTLNNGDQPEFIHPYLIKSSLS 128

Query: 121 SISVSYPSISSKSASICQVFIPDLTISPSDKIDPLPQKSHVISSFNDLNVTLDIPSSNLR 180
           S+SVSYPSISS SASICQVF PDLTISPSDKIDPLPQKSHVISSFNDLNVTLDIPSSNLR
Sbjct: 129 SVSVSYPSISSNSASICQVFTPDLTISPSDKIDPLPQKSHVISSFNDLNVTLDIPSSNLR 188

Query: 181 FYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWIIYSSLPINL 240
           FYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWIIYSSLPINL
Sbjct: 189 FYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWIIYSSLPINL 248

Query: 241 THSLSVITSGGYAGIIRIAALPNNDPECEPILDRFSSCYPISGEVRFTKPFCLEYKWETK 300
           THSLSVITSGGYAGIIRIAALPNNDPECEPILDRFSSCYP+SGEVRFTKPFCLEYKWETK
Sbjct: 249 THSLSVITSGGYAGIIRIAALPNNDPECEPILDRFSSCYPVSGEVRFTKPFCLEYKWETK 308

Query: 301 GWGDLLMLAHPLHLRLLAGTDNDVVILDKFKYRSIDGELVGVVGSSWALKPEPILVSWHS 360
           GWGDLLMLAHPLHLRLLAGTD DVVILDKFKYRSIDGELVGVVGSSWALKPEPILVSWHS
Sbjct: 309 GWGDLLMLAHPLHLRLLAGTDKDVVILDKFKYRSIDGELVGVVGSSWALKPEPILVSWHS 368

Query: 361 MKGVEEESFTDIISALDKDIESLNSTTMTTKSPYSYGKLIARAARLAVIAEEVRYLQVLP 420
           MKGVEEESFTDIISALDKDIESLNSTTMTTKSPYSYGKLIARAARLAVIAEEVRYLQVLP
Sbjct: 369 MKGVEEESFTDIISALDKDIESLNSTTMTTKSPYSYGKLIARAARLAVIAEEVRYLQVLP 428

Query: 421 DIRKFLMGAIEPWLNGTLEGNGFVYDEKWGGIISKEGSFHSGADFGFGIYNNHHHTLGYF 480
           DIRKFLMGAIEPWLNGTLEGNGFVYDEKWGGIISKEGSFHSGADFGFGIYNNHHHTLGYF
Sbjct: 429 DIRKFLMGAIEPWLNGTLEGNGFVYDEKWGGIISKEGSFHSGADFGFGIYNNHHHTLGYF 488

Query: 481 FYAIAVLVKIDPAWGRKYSPQVYALMADIMNLSKQSNSKFPRLRCFDAYKLHSWGTGLAE 540
           FYAIAVLVKIDPAWGRKYSPQVYALMADIMNLS++SNSKFPRLRCFDAYKLHSWGTGLAE
Sbjct: 489 FYAIAVLVKIDPAWGRKYSPQVYALMADIMNLSRRSNSKFPRLRCFDAYKLHSWGTGLAE 548

Query: 541 FMDGRSQESISEAVNAYYSAALVGLAYGDADLVSIGATLAALEIKAGQMWWQVREGQALY 600
           FMDGRSQESISEAVNAYYSAALVGLAYGDADLVSIGATLAALEIKAGQMWWQVREGQALY
Sbjct: 549 FMDGRSQESISEAVNAYYSAALVGLAYGDADLVSIGATLAALEIKAGQMWWQVREGQALY 608

Query: 601 EEEFVKENRLVGVLWSNKRDSGLWFGPGEWKECRLGIQVLPMLPITELLLSDVGFVREAV 660
           EEEFVKENRLVGVLWSNKRDSGLWFGPGEWKECRLGIQVLPMLPITELLLSDVGFVREAV
Sbjct: 609 EEEFVKENRLVGVLWSNKRDSGLWFGPGEWKECRLGIQVLPMLPITELLLSDVGFVREAV 668

Query: 661 NWALPSLGREGVGEAWKGFVYGMESVYDKAGALQKIRNLKAFDDGNSLTNLLWWVHSRPK 720
           NWALPSLGREGVGE WKGFVYGMESVYDKAGALQKIRNL+AFDDGNSLTNLLWWVHSR K
Sbjct: 669 NWALPSLGREGVGEGWKGFVYGMESVYDKAGALQKIRNLEAFDDGNSLTNLLWWVHSRVK 728

Query: 721 EGEANISCTKVQLL 733
           EGEANISCTKVQLL
Sbjct: 729 EGEANISCTKVQLL 742

BLAST of Cp4.1LG13g07980.1 vs. NCBI nr
Match: KAG7016077.1 (Protein trichome birefringence-like 19, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1410 bits (3650), Expect = 0.0
Identity = 716/730 (98.08%), Postives = 721/730 (98.77%), Query Frame = 0

Query: 4    KIERELRRAIKGKRGSPPPRLPPPPPTSPPPPPPPPPPPPEPPQSPPPEPSQKPSEPTPF 63
            KIERELRRAIKGKRGSPPPRLPPPP T  PPPPPPPPPPPEPPQSPPPEPSQKPSEPTPF
Sbjct: 806  KIERELRRAIKGKRGSPPPRLPPPP-T--PPPPPPPPPPPEPPQSPPPEPSQKPSEPTPF 865

Query: 64   LFPSTHSTVLPDPSLFFSPELLSAPLPTNSFFQNFTLNNGDQPEFIHPYLIKSSLSSISV 123
            LFPSTHSTVLPDPSLFFSPELLSAPLPTNSFFQNFTLNNGDQPEFIHPYLIKSSLSSISV
Sbjct: 866  LFPSTHSTVLPDPSLFFSPELLSAPLPTNSFFQNFTLNNGDQPEFIHPYLIKSSLSSISV 925

Query: 124  SYPSISSKSASICQVFIPDLTISPSDKIDPLPQKSHVISSFNDLNVTLDIPSSNLRFYLV 183
            SYPSISS SASICQVF PDLTISPSDKIDPLPQKSHVISSFNDLNVTLDIPSSNLRFYLV
Sbjct: 926  SYPSISSNSASICQVFTPDLTISPSDKIDPLPQKSHVISSFNDLNVTLDIPSSNLRFYLV 985

Query: 184  RGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWIIYSSLPINLTHSL 243
            RGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWIIYSSLPINLTHSL
Sbjct: 986  RGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWIIYSSLPINLTHSL 1045

Query: 244  SVITSGGYAGIIRIAALPNNDPECEPILDRFSSCYPISGEVRFTKPFCLEYKWETKGWGD 303
            SVITSGGYAGIIRIAALPNNDPECEPILDRFSSCYP+SGEV+FTKPFCLEYKWETKGWGD
Sbjct: 1046 SVITSGGYAGIIRIAALPNNDPECEPILDRFSSCYPVSGEVQFTKPFCLEYKWETKGWGD 1105

Query: 304  LLMLAHPLHLRLLAGTDNDVVILDKFKYRSIDGELVGVVGSSWALKPEPILVSWHSMKGV 363
            LLMLAHPLHLRLLAGTDNDVVILDKFKY+SIDGELVGVVGSSWALKPEPILVSWHSMKGV
Sbjct: 1106 LLMLAHPLHLRLLAGTDNDVVILDKFKYKSIDGELVGVVGSSWALKPEPILVSWHSMKGV 1165

Query: 364  EEESFTDIISALDKDIESLNSTTMTTKSPYSYGKLIARAARLAVIAEEVRYLQVLPDIRK 423
            EEESFTDIISALDKDIESLNSTTMTTKSPYSYGKLIARAARLAVIAEEVRYLQVLPDIRK
Sbjct: 1166 EEESFTDIISALDKDIESLNSTTMTTKSPYSYGKLIARAARLAVIAEEVRYLQVLPDIRK 1225

Query: 424  FLMGAIEPWLNGTLEGNGFVYDEKWGGIISKEGSFHSGADFGFGIYNNHHHTLGYFFYAI 483
            FLMGAIEPWLNGTLEGNGFVYDEKWGGIISKEGSFHSGADFGFGIYNNHHHTLGYFFYAI
Sbjct: 1226 FLMGAIEPWLNGTLEGNGFVYDEKWGGIISKEGSFHSGADFGFGIYNNHHHTLGYFFYAI 1285

Query: 484  AVLVKIDPAWGRKYSPQVYALMADIMNLSKQSNSKFPRLRCFDAYKLHSWGTGLAEFMDG 543
            AVLVKIDPAWGRKYSPQVYALMADIMNLS++SNSKFPRLRCFD YKLHSWGTGLAEF DG
Sbjct: 1286 AVLVKIDPAWGRKYSPQVYALMADIMNLSRRSNSKFPRLRCFDTYKLHSWGTGLAEFTDG 1345

Query: 544  RSQESISEAVNAYYSAALVGLAYGDADLVSIGATLAALEIKAGQMWWQVREGQALYEEEF 603
            RSQESISEAVNAYYSAALVGLAYGDADLVSIGATLAALEIKAGQMWWQVREGQALYEEEF
Sbjct: 1346 RSQESISEAVNAYYSAALVGLAYGDADLVSIGATLAALEIKAGQMWWQVREGQALYEEEF 1405

Query: 604  VKENRLVGVLWSNKRDSGLWFGPGEWKECRLGIQVLPMLPITELLLSDVGFVREAVNWAL 663
            VKENRLVGVLWSNKRDSGLWFGPGEWKECRLGIQVLPMLPITELLLSDVGFVREAVNWAL
Sbjct: 1406 VKENRLVGVLWSNKRDSGLWFGPGEWKECRLGIQVLPMLPITELLLSDVGFVREAVNWAL 1465

Query: 664  PSLGREGVGEAWKGFVYGMESVYDKAGALQKIRNLKAFDDGNSLTNLLWWVHSRPKEGEA 723
            PSLGREGVGE WKGFVYGMESVYDKAGALQKIRNLKAFDDGNSLTNLLWWVHSR KEGEA
Sbjct: 1466 PSLGREGVGEGWKGFVYGMESVYDKAGALQKIRNLKAFDDGNSLTNLLWWVHSRVKEGEA 1525

Query: 724  NISCTKVQLL 733
            NISCTKVQLL
Sbjct: 1526 NISCTKVQLL 1532

BLAST of Cp4.1LG13g07980.1 vs. NCBI nr
Match: XP_022993033.1 (endo-1,3(4)-beta-glucanase 2 [Cucurbita maxima])

HSP 1 Score: 1396 bits (3614), Expect = 0.0
Identity = 711/735 (96.73%), Postives = 717/735 (97.55%), Query Frame = 0

Query: 1   MFKKIERELRRAIKGKRGSPPPRLPPPPP--TSPPPPPPPPPPPPEPPQSPPPEPSQKPS 60
           MFKKIERELRRAIKGK GSPPPRLPPPPP  TSPP PPPPP   PEPPQSPPPE SQKPS
Sbjct: 9   MFKKIERELRRAIKGKCGSPPPRLPPPPPPPTSPPCPPPPP---PEPPQSPPPELSQKPS 68

Query: 61  EPTPFLFPSTHSTVLPDPSLFFSPELLSAPLPTNSFFQNFTLNNGDQPEFIHPYLIKSSL 120
           EPTPFLFPSTHSTVLPDPSLFFSP+LLSAPLPTNSFFQNFTLNNGDQPEFIHPYLIKSSL
Sbjct: 69  EPTPFLFPSTHSTVLPDPSLFFSPDLLSAPLPTNSFFQNFTLNNGDQPEFIHPYLIKSSL 128

Query: 121 SSISVSYPSISSKSASICQVFIPDLTISPSDKIDPLPQKSHVISSFNDLNVTLDIPSSNL 180
           SSISVSYPSISS SASICQVF PDLTISPSDKIDPLPQKSHVISSFNDLNVTLDIPSSNL
Sbjct: 129 SSISVSYPSISSNSASICQVFTPDLTISPSDKIDPLPQKSHVISSFNDLNVTLDIPSSNL 188

Query: 181 RFYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWIIYSSLPIN 240
           RFYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWIIYSSLPIN
Sbjct: 189 RFYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWIIYSSLPIN 248

Query: 241 LTHSLSVITSGGYAGIIRIAALPNNDPECEPILDRFSSCYPISGEVRFTKPFCLEYKWET 300
           LTHSL VITSGGYAGIIRIAALPN+DPECE ILDR SSCYP+SGEVRFTKPFCLEYKWET
Sbjct: 249 LTHSLYVITSGGYAGIIRIAALPNSDPECELILDRLSSCYPVSGEVRFTKPFCLEYKWET 308

Query: 301 KGWGDLLMLAHPLHLRLLAGTDNDVVILDKFKYRSIDGELVGVVGSSWALKPEPILVSWH 360
           KGWGDLLMLAHPLHLRLLAGTDND+VILDKFKYRSIDGELVGVVGSSWALKPEPILVSWH
Sbjct: 309 KGWGDLLMLAHPLHLRLLAGTDNDIVILDKFKYRSIDGELVGVVGSSWALKPEPILVSWH 368

Query: 361 SMKGVEEESFTDIISALDKDIESLNSTTMTTKSPYSYGKLIARAARLAVIAEEVRYLQVL 420
           SMKGVEEESFTDIISALDKDIESLNSTTMTTKSPY+YGKLIARAARLAVIAEEVRYLQVL
Sbjct: 369 SMKGVEEESFTDIISALDKDIESLNSTTMTTKSPYAYGKLIARAARLAVIAEEVRYLQVL 428

Query: 421 PDIRKFLMGAIEPWLNGTLEGNGFVYDEKWGGIISKEGSFHSGADFGFGIYNNHHHTLGY 480
           PDIRKFLMGAIEPWLNGTLEGNGFVYDEKWGGIISKEGSFHSGADFGFGIYNNHHHTLGY
Sbjct: 429 PDIRKFLMGAIEPWLNGTLEGNGFVYDEKWGGIISKEGSFHSGADFGFGIYNNHHHTLGY 488

Query: 481 FFYAIAVLVKIDPAWGRKYSPQVYALMADIMNLSKQSNSKFPRLRCFDAYKLHSWGTGLA 540
           FFYAIAVLVKIDPAWGRKYSPQVYALM DIMNLSK+SNSKFPRLRCFD YKLHSWGTGLA
Sbjct: 489 FFYAIAVLVKIDPAWGRKYSPQVYALMGDIMNLSKRSNSKFPRLRCFDGYKLHSWGTGLA 548

Query: 541 EFMDGRSQESISEAVNAYYSAALVGLAYGDADLVSIGATLAALEIKAGQMWWQVREGQAL 600
           EF DGRSQESISEAVNAYYSAALVGLAYGDADLVSIGATLAALEIKAGQMWWQVREGQAL
Sbjct: 549 EFTDGRSQESISEAVNAYYSAALVGLAYGDADLVSIGATLAALEIKAGQMWWQVREGQAL 608

Query: 601 YEEEFVKENRLVGVLWSNKRDSGLWFGPGEWKECRLGIQVLPMLPITELLLSDVGFVREA 660
           YEEEFVKENRLVGVLWSNKRDSGLWFGPGEWKECRLGIQVLPMLPITELLLSDVGFVREA
Sbjct: 609 YEEEFVKENRLVGVLWSNKRDSGLWFGPGEWKECRLGIQVLPMLPITELLLSDVGFVREA 668

Query: 661 VNWALPSLGREGVGEAWKGFVYGMESVYDKAGALQKIRNLKAFDDGNSLTNLLWWVHSRP 720
           VNWALPSLGREGVGE WKGFVYGMESVYDKAGALQKIRNLKAFDDGNSLTNLLWWVHSR 
Sbjct: 669 VNWALPSLGREGVGEGWKGFVYGMESVYDKAGALQKIRNLKAFDDGNSLTNLLWWVHSRA 728

Query: 721 KEGEANISCTKVQLL 733
           KEGEANISCTKVQLL
Sbjct: 729 KEGEANISCTKVQLL 740

BLAST of Cp4.1LG13g07980.1 vs. NCBI nr
Match: KAG6578514.1 (hypothetical protein SDJN03_22962, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1373 bits (3553), Expect = 0.0
Identity = 699/711 (98.31%), Postives = 704/711 (99.02%), Query Frame = 0

Query: 1   MFKKIERELRRAIKGKRGSPPPRLPPPPPTSPPPPPPPPPPPPEPPQSPPPEPSQKPSEP 60
           MFKKIERELRRAIKGKRGSPPPRLPPPP T  PPPPPPPPPPPEPPQSPPPEPSQKPSEP
Sbjct: 1   MFKKIERELRRAIKGKRGSPPPRLPPPP-T--PPPPPPPPPPPEPPQSPPPEPSQKPSEP 60

Query: 61  TPFLFPSTHSTVLPDPSLFFSPELLSAPLPTNSFFQNFTLNNGDQPEFIHPYLIKSSLSS 120
           TPFLFPSTHSTVLPDPSLFFSPELLSAPLPTNSFFQNFTLNNGDQPEFIHPYLIKSSLSS
Sbjct: 61  TPFLFPSTHSTVLPDPSLFFSPELLSAPLPTNSFFQNFTLNNGDQPEFIHPYLIKSSLSS 120

Query: 121 ISVSYPSISSKSASICQVFIPDLTISPSDKIDPLPQKSHVISSFNDLNVTLDIPSSNLRF 180
           ISVSYPSISS SASICQVF PDLTISPSDKIDPLPQKSHVISSFNDLNVTLDIPSSNLRF
Sbjct: 121 ISVSYPSISSNSASICQVFTPDLTISPSDKIDPLPQKSHVISSFNDLNVTLDIPSSNLRF 180

Query: 181 YLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWIIYSSLPINLT 240
           YLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWIIYSSLPINLT
Sbjct: 181 YLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWIIYSSLPINLT 240

Query: 241 HSLSVITSGGYAGIIRIAALPNNDPECEPILDRFSSCYPISGEVRFTKPFCLEYKWETKG 300
           HSLSVITSGGYAGIIRIAALPNNDPECEPILDRFSSCYP+SGEV+FTKPFCLEYKWETKG
Sbjct: 241 HSLSVITSGGYAGIIRIAALPNNDPECEPILDRFSSCYPVSGEVQFTKPFCLEYKWETKG 300

Query: 301 WGDLLMLAHPLHLRLLAGTDNDVVILDKFKYRSIDGELVGVVGSSWALKPEPILVSWHSM 360
           WGDLLMLAHPLHLRLLAGTDNDVVILDKFKY+SIDGELVGVVGSSWALKPEPILVSWHSM
Sbjct: 301 WGDLLMLAHPLHLRLLAGTDNDVVILDKFKYKSIDGELVGVVGSSWALKPEPILVSWHSM 360

Query: 361 KGVEEESFTDIISALDKDIESLNSTTMTTKSPYSYGKLIARAARLAVIAEEVRYLQVLPD 420
           KGVEEESFTDIISALDKDIESLNSTTMTTKSPYSYGKLIARAARLAVIAEEVRYLQVLPD
Sbjct: 361 KGVEEESFTDIISALDKDIESLNSTTMTTKSPYSYGKLIARAARLAVIAEEVRYLQVLPD 420

Query: 421 IRKFLMGAIEPWLNGTLEGNGFVYDEKWGGIISKEGSFHSGADFGFGIYNNHHHTLGYFF 480
           IRKFLMGAIEPWLNGTLEGNGFVYDEKWGGIISKEGSFHSGADFGFGIYNNHHHTLGYFF
Sbjct: 421 IRKFLMGAIEPWLNGTLEGNGFVYDEKWGGIISKEGSFHSGADFGFGIYNNHHHTLGYFF 480

Query: 481 YAIAVLVKIDPAWGRKYSPQVYALMADIMNLSKQSNSKFPRLRCFDAYKLHSWGTGLAEF 540
           YAIAVLVKIDPAWGRKYSPQVYALMADIMNLS++SNSKFPRLRCFDAYKLHSWGTGLAEF
Sbjct: 481 YAIAVLVKIDPAWGRKYSPQVYALMADIMNLSRRSNSKFPRLRCFDAYKLHSWGTGLAEF 540

Query: 541 MDGRSQESISEAVNAYYSAALVGLAYGDADLVSIGATLAALEIKAGQMWWQVREGQALYE 600
           MDGRSQESISEAVNAYYSAALVGLAYGDADLVSIGA LAALEIKAGQMWWQVREGQALYE
Sbjct: 541 MDGRSQESISEAVNAYYSAALVGLAYGDADLVSIGAKLAALEIKAGQMWWQVREGQALYE 600

Query: 601 EEFVKENRLVGVLWSNKRDSGLWFGPGEWKECRLGIQVLPMLPITELLLSDVGFVREAVN 660
           EEFVKENRLVGVLWSNKRDSGLWFGPGEWKECRLGIQVLPMLPITELLLSDVGFVREAVN
Sbjct: 601 EEFVKENRLVGVLWSNKRDSGLWFGPGEWKECRLGIQVLPMLPITELLLSDVGFVREAVN 660

Query: 661 WALPSLGREGVGEAWKGFVYGMESVYDKAGALQKIRNLKAFDDGNSLTNLL 711
           WALPSLGREGVGE WKGFVYGMESVYDKAGALQKIRNLKAFDDGNSLTNLL
Sbjct: 661 WALPSLGREGVGEGWKGFVYGMESVYDKAGALQKIRNLKAFDDGNSLTNLL 708

BLAST of Cp4.1LG13g07980.1 vs. ExPASy TrEMBL
Match: A0A6J1FG59 (Endo-1,3(4)-beta-glucanase OS=Cucurbita moschata OX=3662 GN=LOC111445114 PE=3 SV=1)

HSP 1 Score: 1419 bits (3673), Expect = 0.0
Identity = 719/734 (97.96%), Postives = 724/734 (98.64%), Query Frame = 0

Query: 1   MFKKIERELRRAIKGKRGSPPPRLPPPPPTSPPPPPPPPPPPP-EPPQSPPPEPSQKPSE 60
           MFKKIERELRR  KGKR SPPPRLPPPPPTSPPPPPPPPPPPP EPPQSPPPEPSQ PSE
Sbjct: 9   MFKKIERELRRVTKGKRSSPPPRLPPPPPTSPPPPPPPPPPPPPEPPQSPPPEPSQNPSE 68

Query: 61  PTPFLFPSTHSTVLPDPSLFFSPELLSAPLPTNSFFQNFTLNNGDQPEFIHPYLIKSSLS 120
           PTPFLFPSTHSTVLPDPSLFFSPELLSAPLPTNSFFQNFTLNNGDQPEFIHPYLIKSSLS
Sbjct: 69  PTPFLFPSTHSTVLPDPSLFFSPELLSAPLPTNSFFQNFTLNNGDQPEFIHPYLIKSSLS 128

Query: 121 SISVSYPSISSKSASICQVFIPDLTISPSDKIDPLPQKSHVISSFNDLNVTLDIPSSNLR 180
           S+SVSYPSISS SASICQVF PDLTISPSDKIDPLPQKSHVISSFNDLNVTLDIPSSNLR
Sbjct: 129 SVSVSYPSISSNSASICQVFTPDLTISPSDKIDPLPQKSHVISSFNDLNVTLDIPSSNLR 188

Query: 181 FYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWIIYSSLPINL 240
           FYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWIIYSSLPINL
Sbjct: 189 FYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWIIYSSLPINL 248

Query: 241 THSLSVITSGGYAGIIRIAALPNNDPECEPILDRFSSCYPISGEVRFTKPFCLEYKWETK 300
           THSLSVITSGGYAGIIRIAALPNNDPECEPILDRFSSCYP+SGEVRFTKPFCLEYKWETK
Sbjct: 249 THSLSVITSGGYAGIIRIAALPNNDPECEPILDRFSSCYPVSGEVRFTKPFCLEYKWETK 308

Query: 301 GWGDLLMLAHPLHLRLLAGTDNDVVILDKFKYRSIDGELVGVVGSSWALKPEPILVSWHS 360
           GWGDLLMLAHPLHLRLLAGTD DVVILDKFKYRSIDGELVGVVGSSWALKPEPILVSWHS
Sbjct: 309 GWGDLLMLAHPLHLRLLAGTDKDVVILDKFKYRSIDGELVGVVGSSWALKPEPILVSWHS 368

Query: 361 MKGVEEESFTDIISALDKDIESLNSTTMTTKSPYSYGKLIARAARLAVIAEEVRYLQVLP 420
           MKGVEEESFTDIISALDKDIESLNSTTMTTKSPYSYGKLIARAARLAVIAEEVRYLQVLP
Sbjct: 369 MKGVEEESFTDIISALDKDIESLNSTTMTTKSPYSYGKLIARAARLAVIAEEVRYLQVLP 428

Query: 421 DIRKFLMGAIEPWLNGTLEGNGFVYDEKWGGIISKEGSFHSGADFGFGIYNNHHHTLGYF 480
           DIRKFLMGAIEPWLNGTLEGNGFVYDEKWGGIISKEGSFHSGADFGFGIYNNHHHTLGYF
Sbjct: 429 DIRKFLMGAIEPWLNGTLEGNGFVYDEKWGGIISKEGSFHSGADFGFGIYNNHHHTLGYF 488

Query: 481 FYAIAVLVKIDPAWGRKYSPQVYALMADIMNLSKQSNSKFPRLRCFDAYKLHSWGTGLAE 540
           FYAIAVLVKIDPAWGRKYSPQVYALMADIMNLS++SNSKFPRLRCFDAYKLHSWGTGLAE
Sbjct: 489 FYAIAVLVKIDPAWGRKYSPQVYALMADIMNLSRRSNSKFPRLRCFDAYKLHSWGTGLAE 548

Query: 541 FMDGRSQESISEAVNAYYSAALVGLAYGDADLVSIGATLAALEIKAGQMWWQVREGQALY 600
           FMDGRSQESISEAVNAYYSAALVGLAYGDADLVSIGATLAALEIKAGQMWWQVREGQALY
Sbjct: 549 FMDGRSQESISEAVNAYYSAALVGLAYGDADLVSIGATLAALEIKAGQMWWQVREGQALY 608

Query: 601 EEEFVKENRLVGVLWSNKRDSGLWFGPGEWKECRLGIQVLPMLPITELLLSDVGFVREAV 660
           EEEFVKENRLVGVLWSNKRDSGLWFGPGEWKECRLGIQVLPMLPITELLLSDVGFVREAV
Sbjct: 609 EEEFVKENRLVGVLWSNKRDSGLWFGPGEWKECRLGIQVLPMLPITELLLSDVGFVREAV 668

Query: 661 NWALPSLGREGVGEAWKGFVYGMESVYDKAGALQKIRNLKAFDDGNSLTNLLWWVHSRPK 720
           NWALPSLGREGVGE WKGFVYGMESVYDKAGALQKIRNL+AFDDGNSLTNLLWWVHSR K
Sbjct: 669 NWALPSLGREGVGEGWKGFVYGMESVYDKAGALQKIRNLEAFDDGNSLTNLLWWVHSRVK 728

Query: 721 EGEANISCTKVQLL 733
           EGEANISCTKVQLL
Sbjct: 729 EGEANISCTKVQLL 742

BLAST of Cp4.1LG13g07980.1 vs. ExPASy TrEMBL
Match: A0A6J1K0Z9 (Endo-1,3(4)-beta-glucanase OS=Cucurbita maxima OX=3661 GN=LOC111489174 PE=3 SV=1)

HSP 1 Score: 1396 bits (3614), Expect = 0.0
Identity = 711/735 (96.73%), Postives = 717/735 (97.55%), Query Frame = 0

Query: 1   MFKKIERELRRAIKGKRGSPPPRLPPPPP--TSPPPPPPPPPPPPEPPQSPPPEPSQKPS 60
           MFKKIERELRRAIKGK GSPPPRLPPPPP  TSPP PPPPP   PEPPQSPPPE SQKPS
Sbjct: 9   MFKKIERELRRAIKGKCGSPPPRLPPPPPPPTSPPCPPPPP---PEPPQSPPPELSQKPS 68

Query: 61  EPTPFLFPSTHSTVLPDPSLFFSPELLSAPLPTNSFFQNFTLNNGDQPEFIHPYLIKSSL 120
           EPTPFLFPSTHSTVLPDPSLFFSP+LLSAPLPTNSFFQNFTLNNGDQPEFIHPYLIKSSL
Sbjct: 69  EPTPFLFPSTHSTVLPDPSLFFSPDLLSAPLPTNSFFQNFTLNNGDQPEFIHPYLIKSSL 128

Query: 121 SSISVSYPSISSKSASICQVFIPDLTISPSDKIDPLPQKSHVISSFNDLNVTLDIPSSNL 180
           SSISVSYPSISS SASICQVF PDLTISPSDKIDPLPQKSHVISSFNDLNVTLDIPSSNL
Sbjct: 129 SSISVSYPSISSNSASICQVFTPDLTISPSDKIDPLPQKSHVISSFNDLNVTLDIPSSNL 188

Query: 181 RFYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWIIYSSLPIN 240
           RFYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWIIYSSLPIN
Sbjct: 189 RFYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWIIYSSLPIN 248

Query: 241 LTHSLSVITSGGYAGIIRIAALPNNDPECEPILDRFSSCYPISGEVRFTKPFCLEYKWET 300
           LTHSL VITSGGYAGIIRIAALPN+DPECE ILDR SSCYP+SGEVRFTKPFCLEYKWET
Sbjct: 249 LTHSLYVITSGGYAGIIRIAALPNSDPECELILDRLSSCYPVSGEVRFTKPFCLEYKWET 308

Query: 301 KGWGDLLMLAHPLHLRLLAGTDNDVVILDKFKYRSIDGELVGVVGSSWALKPEPILVSWH 360
           KGWGDLLMLAHPLHLRLLAGTDND+VILDKFKYRSIDGELVGVVGSSWALKPEPILVSWH
Sbjct: 309 KGWGDLLMLAHPLHLRLLAGTDNDIVILDKFKYRSIDGELVGVVGSSWALKPEPILVSWH 368

Query: 361 SMKGVEEESFTDIISALDKDIESLNSTTMTTKSPYSYGKLIARAARLAVIAEEVRYLQVL 420
           SMKGVEEESFTDIISALDKDIESLNSTTMTTKSPY+YGKLIARAARLAVIAEEVRYLQVL
Sbjct: 369 SMKGVEEESFTDIISALDKDIESLNSTTMTTKSPYAYGKLIARAARLAVIAEEVRYLQVL 428

Query: 421 PDIRKFLMGAIEPWLNGTLEGNGFVYDEKWGGIISKEGSFHSGADFGFGIYNNHHHTLGY 480
           PDIRKFLMGAIEPWLNGTLEGNGFVYDEKWGGIISKEGSFHSGADFGFGIYNNHHHTLGY
Sbjct: 429 PDIRKFLMGAIEPWLNGTLEGNGFVYDEKWGGIISKEGSFHSGADFGFGIYNNHHHTLGY 488

Query: 481 FFYAIAVLVKIDPAWGRKYSPQVYALMADIMNLSKQSNSKFPRLRCFDAYKLHSWGTGLA 540
           FFYAIAVLVKIDPAWGRKYSPQVYALM DIMNLSK+SNSKFPRLRCFD YKLHSWGTGLA
Sbjct: 489 FFYAIAVLVKIDPAWGRKYSPQVYALMGDIMNLSKRSNSKFPRLRCFDGYKLHSWGTGLA 548

Query: 541 EFMDGRSQESISEAVNAYYSAALVGLAYGDADLVSIGATLAALEIKAGQMWWQVREGQAL 600
           EF DGRSQESISEAVNAYYSAALVGLAYGDADLVSIGATLAALEIKAGQMWWQVREGQAL
Sbjct: 549 EFTDGRSQESISEAVNAYYSAALVGLAYGDADLVSIGATLAALEIKAGQMWWQVREGQAL 608

Query: 601 YEEEFVKENRLVGVLWSNKRDSGLWFGPGEWKECRLGIQVLPMLPITELLLSDVGFVREA 660
           YEEEFVKENRLVGVLWSNKRDSGLWFGPGEWKECRLGIQVLPMLPITELLLSDVGFVREA
Sbjct: 609 YEEEFVKENRLVGVLWSNKRDSGLWFGPGEWKECRLGIQVLPMLPITELLLSDVGFVREA 668

Query: 661 VNWALPSLGREGVGEAWKGFVYGMESVYDKAGALQKIRNLKAFDDGNSLTNLLWWVHSRP 720
           VNWALPSLGREGVGE WKGFVYGMESVYDKAGALQKIRNLKAFDDGNSLTNLLWWVHSR 
Sbjct: 669 VNWALPSLGREGVGEGWKGFVYGMESVYDKAGALQKIRNLKAFDDGNSLTNLLWWVHSRA 728

Query: 721 KEGEANISCTKVQLL 733
           KEGEANISCTKVQLL
Sbjct: 729 KEGEANISCTKVQLL 740

BLAST of Cp4.1LG13g07980.1 vs. ExPASy TrEMBL
Match: A0A6J1BXH2 (Endo-1,3(4)-beta-glucanase OS=Momordica charantia OX=3673 GN=LOC111006593 PE=3 SV=1)

HSP 1 Score: 1263 bits (3267), Expect = 0.0
Identity = 632/733 (86.22%), Postives = 677/733 (92.36%), Query Frame = 0

Query: 1   MFKKIERELRRAIKGKRGSPPPRLPPPPPTSPPPPPPPPPPPPEPPQSPPPEPSQKPSEP 60
           MFKKIERELRRAIKGKR S PP+  PPPP  PPPPPPP PPP +PP+SPPPEPSQKPSEP
Sbjct: 1   MFKKIERELRRAIKGKRRSSPPQRLPPPPPPPPPPPPPSPPPQQPPKSPPPEPSQKPSEP 60

Query: 61  TPFLFPSTHSTVLPDPSLFFSPELLSAPLPTNSFFQNFTLNNGDQPEFIHPYLIKSSLSS 120
           TPFLFP T STVLPDPS FFS ELLSAPLPTNSFFQNFTLNNGDQPE+IHPYLIKSSLSS
Sbjct: 61  TPFLFPPTQSTVLPDPSPFFSHELLSAPLPTNSFFQNFTLNNGDQPEYIHPYLIKSSLSS 120

Query: 121 ISVSYPSISSKSASICQVFIPDLTISPSDKIDPLPQKSHVISSFNDLNVTLDIPSSNLRF 180
           ISVSYPSISS SAS  Q+F PDLTI PS+KI+PLPQKSHVISSFNDLNVTLDIPSSNLRF
Sbjct: 121 ISVSYPSISSNSASESQIFTPDLTIYPSEKIEPLPQKSHVISSFNDLNVTLDIPSSNLRF 180

Query: 181 YLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWIIYSSLPINLT 240
           +LVRGSPFLTFTVSKG AFSIS  HEV+SFSFNNALTKYT+KLKNNQTW+IYSS PINLT
Sbjct: 181 FLVRGSPFLTFTVSKGAAFSISASHEVLSFSFNNALTKYTVKLKNNQTWLIYSSCPINLT 240

Query: 241 HSLSVITSGGYAGIIRIAALPNNDPECEPILDRFSSCYPISGEVRFTKPFCLEYKWETKG 300
           H+LSVITSGG+AGIIRIAALPN+DPECEPILDRFSSCYP+SGE  FTKPFC+EYKWET+G
Sbjct: 241 HNLSVITSGGFAGIIRIAALPNSDPECEPILDRFSSCYPVSGEALFTKPFCMEYKWETRG 300

Query: 301 WGDLLMLAHPLHLRLLAGTDNDVVILDKFKYRSIDGELVGVVGSSWALKPEPILVSWHSM 360
           WGDLLMLAHPLH+RLLAGT+NDVVILDKFKY+SIDG+LVGVVGS W LKPEP+ V WHS+
Sbjct: 301 WGDLLMLAHPLHIRLLAGTNNDVVILDKFKYKSIDGDLVGVVGSLWVLKPEPVSVCWHSI 360

Query: 361 KGVEEESFTDIISALDKDIESLNSTTMTTKSPYSYGKLIARAARLAVIAEEVRYLQVLPD 420
           +GVEEESF +IISAL KD+E+LNSTTMTT SPYSYGKLIARAARLAVIAEEVR L+V+P+
Sbjct: 361 RGVEEESFAEIISALRKDVEALNSTTMTTTSPYSYGKLIARAARLAVIAEEVRCLEVIPE 420

Query: 421 IRKFLMGAIEPWLNGTLEGNGFVYDEKWGGIISKEGSFHSGADFGFGIYNNHHHTLGYFF 480
           IRKFLMGAIEPWLNGT +GNGFVYD KWGGI+SKEGSF S ADFGFGIYNNHHH LGYF 
Sbjct: 421 IRKFLMGAIEPWLNGTFDGNGFVYDGKWGGIVSKEGSFDSVADFGFGIYNNHHHHLGYFL 480

Query: 481 YAIAVLVKIDPAWGRKYSPQVYALMADIMNLSKQSNSKFPRLRCFDAYKLHSWGTGLAEF 540
           YAIAVLVKIDPAWGRKYSPQ Y+LMADIMNLS++SNSKFPRLRCFD YKLHSWGTGL EF
Sbjct: 481 YAIAVLVKIDPAWGRKYSPQAYSLMADIMNLSRRSNSKFPRLRCFDVYKLHSWGTGLTEF 540

Query: 541 MDGRSQESISEAVNAYYSAALVGLAYGDADLVSIGATLAALEIKAGQMWWQVREGQALYE 600
           MDGR QESISEAVNAYYSAALVGLAYGDA LVSIG+ LAALEIKAGQMWWQVREG+ LY+
Sbjct: 541 MDGRGQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGETLYQ 600

Query: 601 EEFVKENRLVGVLWSNKRDSGLWFGPGEWKECRLGIQVLPMLPITELLLSDVGFVREAVN 660
           EEFVKENRLVGVLWSNKRDSGLWF P EW+EC+LGIQVLP+LPITELL SDVGFVRE VN
Sbjct: 601 EEFVKENRLVGVLWSNKRDSGLWFAPREWRECKLGIQVLPVLPITELLFSDVGFVRELVN 660

Query: 661 WALPSLGREGVGEAWKGFVYGMESVYDKAGALQKIRNLKAFDDGNSLTNLLWWVHSRPKE 720
           W LPSLGREGVGE WKGFVY +ES+YDK GAL+KIRNLK FDDGNSLTNLLWWVHSR KE
Sbjct: 661 WTLPSLGREGVGEGWKGFVYALESIYDKDGALEKIRNLKDFDDGNSLTNLLWWVHSRGKE 720

Query: 721 GEANISCTKVQLL 733
           GEANIS  KVQL+
Sbjct: 721 GEANISSNKVQLV 733

BLAST of Cp4.1LG13g07980.1 vs. ExPASy TrEMBL
Match: A0A5A7T8V6 (Endo-1,3(4)-beta-glucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001970 PE=3 SV=1)

HSP 1 Score: 1246 bits (3224), Expect = 0.0
Identity = 628/736 (85.33%), Postives = 681/736 (92.53%), Query Frame = 0

Query: 1   MFKKIERELRRAIKGKRGSPPPRLPPPPPTSPPPPPPPPPPPPEPPQSPPPEPSQKPSEP 60
           MFKKIERELRRA KGKR SPP RLPPPPP        PPPPPP+ P+S PPEPSQKPSEP
Sbjct: 1   MFKKIERELRRAFKGKRHSPPLRLPPPPP--------PPPPPPQTPESQPPEPSQKPSEP 60

Query: 61  TPFLFPSTHSTVLPDPSLFFSPELLSAPLPTNSFFQNFTLNNGDQPEFIHPYLIKSSLSS 120
           TPFLFPST STVLPDPS+FFSPELLSAPLP+NSFFQNFTLNNGDQPE+IHPYLIKSSLSS
Sbjct: 61  TPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSS 120

Query: 121 ISVSYPSISSKSASICQVFIPDLTISPSDKIDPLPQKSHVISSFNDLNVTLDIPSSNLRF 180
           ISVSYPSI S  AS CQ+F PDLTISPS+KI+PLPQKSHVISSFNDL+VTLDIPSSNLRF
Sbjct: 121 ISVSYPSILSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRF 180

Query: 181 YLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWIIYSSLPINLT 240
           YLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTW+IYSS PINLT
Sbjct: 181 YLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLT 240

Query: 241 HSLSVITSGGYAGIIRIAALPNNDPECEPILDRFSSCYPISGEVRFTKPFCLEYKWETKG 300
           H+LS+ITSGG+AGIIRIAALPN+D ECE ILDRFSSCYP+ GE +FTKPFCLEYKWETKG
Sbjct: 241 HNLSMITSGGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKG 300

Query: 301 WGDLLMLAHPLHLRLLAGTDNDVVILDKFKYRSIDGELVGVVGSSWALKPEPILVSWHSM 360
           WGDLLMLAHPLHLRLL G+D++V+ILDKFKY+SIDGELVGVVGSSWALKPEPI VSWHS+
Sbjct: 301 WGDLLMLAHPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSI 360

Query: 361 KGVEEESFTDIISALDKDIESLNSTTM--TTKSPYSYGKLIARAARLAVIAEEVRYLQVL 420
           +GVEEESF +IISAL KD+E+LNST+M  TTKSPYSYGKLIARAARLAVIAEEVR L+V+
Sbjct: 361 RGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVV 420

Query: 421 PDIRKFLMGAIEPWLNGTLEGNGFVYDEKWGGIISKEGSFHSGADFGFGIYNNHHHTLGY 480
           P+IRKFLMGAIEPWLNGT EGNGF+YDEKWGGI++KEG+F   ADFGFGIYNNHHH LGY
Sbjct: 421 PEIRKFLMGAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGY 480

Query: 481 FFYAIAVLVKIDPAWGRKYSPQVYALMADIMNLSKQSNSKFPRLRCFDAYKLHSWGTGLA 540
           F YAIAVLVKIDPAWGRKYSPQVY+LMADIMNLS+++NSKFP+LRCFD YKLHSWGTGLA
Sbjct: 481 FLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLA 540

Query: 541 EFMDGRSQESISEAVNAYYSAALVGLAYGDADLVSIGATLAALEIKAGQMWWQVREGQA- 600
           EF DGRSQES+SEAVNAYYSAALVGLAYGDA LVSIG+ LAALEIKAGQMWWQ+REG+  
Sbjct: 541 EFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETT 600

Query: 601 LYEEEFVKENRLVGVLWSNKRDSGLWFGPGEWKECRLGIQVLPMLPITELLLSDVGFVRE 660
           LY+EEFVKENR+VGVLWSNKRDSGLWF P EWKECRLGIQVLP+LPITELLLSDVGFVRE
Sbjct: 601 LYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRE 660

Query: 661 AVNWALPSLGREGVGEAWKGFVYGMESVYDKAGALQKIRNLKAFDDGNSLTNLLWWVHSR 720
            VNWALPSLGREGVGE WKGFV+ +ES+YDK G+LQKIRNLK FDDGNSLTNLLWWVHSR
Sbjct: 661 LVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHSR 720

Query: 721 PKEGEANISCTKVQLL 733
            KE E NIS +K+ L+
Sbjct: 721 GKE-EENISNSKIHLV 727

BLAST of Cp4.1LG13g07980.1 vs. ExPASy TrEMBL
Match: E5GCT6 (Endo-1,3(4)-beta-glucanase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 1244 bits (3220), Expect = 0.0
Identity = 627/736 (85.19%), Postives = 681/736 (92.53%), Query Frame = 0

Query: 1   MFKKIERELRRAIKGKRGSPPPRLPPPPPTSPPPPPPPPPPPPEPPQSPPPEPSQKPSEP 60
           MFKKIERELRRA KGKR SPP RLPPPPP        PPPPPP+ P+S PPEPSQKPSEP
Sbjct: 1   MFKKIERELRRAFKGKRHSPPLRLPPPPP--------PPPPPPQTPESQPPEPSQKPSEP 60

Query: 61  TPFLFPSTHSTVLPDPSLFFSPELLSAPLPTNSFFQNFTLNNGDQPEFIHPYLIKSSLSS 120
           TPFLFPST STVLPDPS+FFSPELLSAPLP+NSFFQNFTLNNGDQPE+IHPYLIKSSLSS
Sbjct: 61  TPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSS 120

Query: 121 ISVSYPSISSKSASICQVFIPDLTISPSDKIDPLPQKSHVISSFNDLNVTLDIPSSNLRF 180
           ISVSYPSI S  AS CQ+F PDLTISPS+KI+PLPQKSHVISSFNDL+VTLDIPSSNLRF
Sbjct: 121 ISVSYPSILSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRF 180

Query: 181 YLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWIIYSSLPINLT 240
           YLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTW+IYSS PINLT
Sbjct: 181 YLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLT 240

Query: 241 HSLSVITSGGYAGIIRIAALPNNDPECEPILDRFSSCYPISGEVRFTKPFCLEYKWETKG 300
           H+LS+ITSGG+AGIIRIAALPN+D ECE ILDRFSSCYP+ GE +FTKPFCLEYKWETKG
Sbjct: 241 HNLSMITSGGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKG 300

Query: 301 WGDLLMLAHPLHLRLLAGTDNDVVILDKFKYRSIDGELVGVVGSSWALKPEPILVSWHSM 360
           WGDLLMLAHPLHLRLL G+D++V+ILDKFKY+SIDGELVGVVGSSWALKPEPI VSWHS+
Sbjct: 301 WGDLLMLAHPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSI 360

Query: 361 KGVEEESFTDIISALDKDIESLNSTTM--TTKSPYSYGKLIARAARLAVIAEEVRYLQVL 420
           +GVEEESF +IISAL KD+E+LNST+M  TTKSPYSYGKLIARAARLAVIAEEVR L+V+
Sbjct: 361 RGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVV 420

Query: 421 PDIRKFLMGAIEPWLNGTLEGNGFVYDEKWGGIISKEGSFHSGADFGFGIYNNHHHTLGY 480
           P+IRKFL+GAIEPWLNGT EGNGF+YDEKWGGI++KEG+F   ADFGFGIYNNHHH LGY
Sbjct: 421 PEIRKFLIGAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGY 480

Query: 481 FFYAIAVLVKIDPAWGRKYSPQVYALMADIMNLSKQSNSKFPRLRCFDAYKLHSWGTGLA 540
           F YAIAVLVKIDPAWGRKYSPQVY+LMADIMNLS+++NSKFP+LRCFD YKLHSWGTGLA
Sbjct: 481 FLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLA 540

Query: 541 EFMDGRSQESISEAVNAYYSAALVGLAYGDADLVSIGATLAALEIKAGQMWWQVREGQA- 600
           EF DGRSQES+SEAVNAYYSAALVGLAYGDA LVSIG+ LAALEIKAGQMWWQ+REG+  
Sbjct: 541 EFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETT 600

Query: 601 LYEEEFVKENRLVGVLWSNKRDSGLWFGPGEWKECRLGIQVLPMLPITELLLSDVGFVRE 660
           LY+EEFVKENR+VGVLWSNKRDSGLWF P EWKECRLGIQVLP+LPITELLLSDVGFVRE
Sbjct: 601 LYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRE 660

Query: 661 AVNWALPSLGREGVGEAWKGFVYGMESVYDKAGALQKIRNLKAFDDGNSLTNLLWWVHSR 720
            VNWALPSLGREGVGE WKGFV+ +ES+YDK G+LQKIRNLK FDDGNSLTNLLWWVHSR
Sbjct: 661 LVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHSR 720

Query: 721 PKEGEANISCTKVQLL 733
            KE E NIS +K+ L+
Sbjct: 721 GKE-EENISNSKIHLV 727

BLAST of Cp4.1LG13g07980.1 vs. TAIR 10
Match: AT5G15870.1 (glycosyl hydrolase family 81 protein )

HSP 1 Score: 880.6 bits (2274), Expect = 9.0e-256
Identity = 453/727 (62.31%), Postives = 554/727 (76.20%), Query Frame = 0

Query: 1   MFKKIERELRRAIKGKRGSPPPRLPPPPPTSPPPPPPPPPPPPEPPQSPPPEPSQKPSEP 60
           M KK+ R+++  I     + P + P   P SPPPP P PP P  PP        QK    
Sbjct: 1   MLKKVRRKVKVLI-----TKPFKKPKNRPPSPPPPLPLPPSPSPPPSQQMSSSRQK---N 60

Query: 61  TPFLFPSTHSTVLPDPSLFFSPELLSAPLPTNSFFQNFTLNNGDQPEFIHPYLIKSSLSS 120
           TPFLFP + S+VLPDPS FFS +LLS+PLPTNSFFQNFTLNNGDQ E+ HPY+IK S SS
Sbjct: 61  TPFLFPRSDSSVLPDPSRFFSHDLLSSPLPTNSFFQNFTLNNGDQAEYFHPYIIKPSTSS 120

Query: 121 ISVSYPSISSKSASICQVFIPDLTISPSDKIDPLPQKSHVISSFNDLNVTLDIPSSNLRF 180
           +S+SYPS+S  SA I + F  D+TI+ SD  DP  +KSH+ISSF+DL VTLD PSSNLRF
Sbjct: 121 LSISYPSLSHNSAFIYEAFNADITITGSDGPDPHSRKSHLISSFSDLGVTLDFPSSNLRF 180

Query: 181 YLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWIIYSSLPINLT 240
           +LVRGSPF+TF+V+  +  +ISTIH V+S S N + TKYT+KL NNQTW+IY+S PINLT
Sbjct: 181 FLVRGSPFITFSVNSSI--TISTIHAVLSLSGNTSSTKYTVKLNNNQTWLIYASSPINLT 240

Query: 241 -HSLSVITSG-GYAGIIRIAALPNNDPECEPILDRFSSCYPISGEVRFTKPFCLEYKWET 300
              +S I  G G++GIIRI  LPN +P  E ILD FS  YP+SG+  FTKPF LEYKWE 
Sbjct: 241 KDGVSSINCGDGFSGIIRIVVLPNPNPYFETILDGFSCSYPVSGDADFTKPFALEYKWEK 300

Query: 301 KGWGDLLMLAHPLHLRLLAGTDNDVVILDKFKYRSIDGELVGVVGSSWALKPEPILVSWH 360
           +G+GDLLMLAHPLHL+LL+  D  + +LD FKY SIDG+LVGV+G SW LKP+P+ V+WH
Sbjct: 301 RGYGDLLMLAHPLHLKLLSTNDCSITVLDNFKYNSIDGDLVGVIGDSWVLKPDPVSVTWH 360

Query: 361 SMKGVEEESFTDIISALDKDIESLNSTTMTTKSPYSYGKLIARAARLAVIAEEVRYLQVL 420
           S+KGV+E+S  +IISAL KD+ +L+S+   T S Y Y KLIARAARLA+IAEEV YL V+
Sbjct: 361 SIKGVQEDSHQEIISALIKDVNALDSSAEVTNSSYFYAKLIARAARLALIAEEVCYLDVI 420

Query: 421 PDIRKFLMGAIEPWLNGTLEGNGFVYDEKWGGIISKEGSFHSGADFGFGIYNNHHHTLGY 480
           P IR +L   IEPWLNG+   NGF+YD KWGG+I+K GS  SGADFGFGIYN+HH+ LGY
Sbjct: 421 PKIRTYLKNMIEPWLNGSFGPNGFLYDPKWGGVITKLGSRDSGADFGFGIYNDHHYHLGY 480

Query: 481 FFYAIAVLVKIDPAWGRKYSPQVYALMADIMNLSK---QSNSKFPRLRCFDAYKLHSWGT 540
           F YAIAVL KIDP WG++Y PQ Y LMAD + L K   +SNS +PRLRCFD +KLHSW  
Sbjct: 481 FVYAIAVLAKIDPLWGKRYRPQAYTLMADYLTLGKKGAKSNSNYPRLRCFDLFKLHSWAG 540

Query: 541 GLAEFMDGRSQESISEAVNAYYSAALVGLAYGDADLVSIGATLAALEIKAGQMWWQVREG 600
           GL EF DGR+QES SEAVNAYYSAAL+GLAYGD  LV+  + +  LEI A +MWWQV+E 
Sbjct: 541 GLTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLVAAASMVLTLEIHAAKMWWQVKED 600

Query: 601 QALYEEEFVKENRLVGVLWSNKRDSGLWFGPGEWKECRLGIQVLPMLPITELLLSDVGFV 660
            A+Y ++F  ENR+VGVLWS KRDSGLWF P EWKECRLGIQ+LP+LP++E+L SDV FV
Sbjct: 601 DAIYPQDFTSENRVVGVLWSTKRDSGLWFAPKEWKECRLGIQLLPLLPVSEVLFSDVTFV 660

Query: 661 REAVNWALPSLGREGVGEAWKGFVYGMESVYDKAGALQKIRNLKAFDDGNSLTNLLWWVH 720
           ++ VNW +P+L R+ VGE WKGFVY +ES+YDK GA++KI+ L  FDDGNSL+NLLWWVH
Sbjct: 661 KQLVNWTMPALARDSVGEGWKGFVYALESMYDKDGAMEKIKGLNGFDDGNSLSNLLWWVH 717

Query: 721 SRPKEGE 723
           SR  + +
Sbjct: 721 SRNNDDD 717

BLAST of Cp4.1LG13g07980.1 vs. TAIR 10
Match: AT1G18310.1 (glycosyl hydrolase family 81 protein )

HSP 1 Score: 790.8 bits (2041), Expect = 9.4e-229
Identity = 394/681 (57.86%), Postives = 484/681 (71.07%), Query Frame = 0

Query: 63  FLFPSTHSTVLPDPSLFFSPELLSAPLPTNSFFQNFTLNNGDQPEFIHPYLIKSSLSSIS 122
           FLFP T S+VLPDPS FFSP+LLS PLPTNSFFQNFTL NGDQ E+ HPYLIKS  SS+ 
Sbjct: 8   FLFPETKSSVLPDPSRFFSPDLLSNPLPTNSFFQNFTLKNGDQAEYFHPYLIKSVDSSLC 67

Query: 123 VSYPSISSKSASICQVFIPDLTISPSDKIDPLPQKSHVISSFNDLNVTLDIPSSNLRFYL 182
           +SYPS+   S    +VF  D+ IS S+   P  +K+H+ISSF+DL VTLD PSSNLRF+L
Sbjct: 68  ISYPSLFHNSDFFYEVFTADIIISGSNGPGPRSRKTHIISSFSDLVVTLDFPSSNLRFFL 127

Query: 183 VRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWIIYSSLPINLTH- 242
           VRGSP                          ++ TK++ KL NNQ W+IY+S PI+LT  
Sbjct: 128 VRGSP--------------------------SSSTKWSDKLTNNQRWLIYASSPIDLTKH 187

Query: 243 -SLSVITSGGYAGIIRIAALPNNDPECEPILDRFSSCYPISGEVRFTKPFCLEYKWETKG 302
              S+   GG+ GI+RI  LP ++P  E  LDRFSSCYP+SG+  FTKPF L+Y WE +G
Sbjct: 188 GDSSIHCRGGFTGIVRITVLPGSNPGYESTLDRFSSCYPVSGDADFTKPFTLKYMWEKRG 247

Query: 303 WGDLLMLAHPLHLRLLAG--------------------TDNDVVILDKFKYRSIDGELVG 362
            GDLLMLAHPLHL+LLA                     T++ V +LD F+Y+SIDG+LVG
Sbjct: 248 SGDLLMLAHPLHLKLLAKDASSLFQQSTLLLVCPDRGCTNSSVTVLDHFRYKSIDGDLVG 307

Query: 363 VVGSSWALKPEPILVSWHSMKGVEEESFTDIISALDKDIESLNSTTMTTKSPYSYGKLIA 422
           VVG SW LKP+ + V+WHSMKGV+++S+ +IISAL KD+  LNS+   T S Y YGKLIA
Sbjct: 308 VVGDSWVLKPDSVSVTWHSMKGVKKDSYKEIISALGKDVNGLNSSAEVTSSSYFYGKLIA 367

Query: 423 RAARLAVIAEEVRYLQVLPDIRKFLMGAIEPWLNGTLEGNGFVYDEKWGGIISKEGSFHS 482
           RAAR A+IAEEV YL V+P I  +L   IEPWL+G+ + NGF+YD KWGG+I+K+GS  S
Sbjct: 368 RAARFALIAEEVCYLDVIPKIVTYLKNMIEPWLDGSFKPNGFLYDPKWGGLITKQGSKDS 427

Query: 483 GADFGFGIYNNHHHTLGYFFYAIAVLVKIDPAWGRKYSPQVYALMADIMNLSKQ----SN 542
            ADFGFGIYN+HH+ +GYF YAIAVL K DP WG +Y  Q Y+L+AD M   ++    SN
Sbjct: 428 QADFGFGIYNDHHYHIGYFLYAIAVLAKFDPLWGERYRAQAYSLLADFMTFGRKDDNNSN 487

Query: 543 SKFPRLRCFDAYKLHSWGTGLAEFMDGRSQESISEAVNAYYSAALVGLAYGDADLVSIGA 602
           S +PRLR FD +KLHSW  GL EF DGR+QES SEAVNAYYSAAL+GLAYGD  LV   +
Sbjct: 488 SSYPRLRNFDLFKLHSWAGGLTEFWDGRNQESTSEAVNAYYSAALLGLAYGDKHLVETAS 547

Query: 603 TLAALEIKAGQMWWQVREGQALYEEEFVKENRLVGVLWSNKRDSGLWFGPGEWKECRLGI 662
           T+  LEI A +MWWQV++G+ALY ++F  ENR+VGVLWS KRDS LWFGP EWKECRLGI
Sbjct: 548 TIMTLEIHAAKMWWQVKKGEALYPKDFTAENRVVGVLWSTKRDSSLWFGPKEWKECRLGI 607

Query: 663 QVLPMLPITELLLSDVGFVREAVNWALPSLGREGVGEAWKGFVYGMESVYDKAGALQKIR 718
           Q+LP+LP+              VNW LP+L R GVGE WKGF+Y +ES+YDK GA++KI+
Sbjct: 608 QLLPILPL--------------VNWTLPALQRNGVGEGWKGFLYALESLYDKDGAIKKIK 648