Cp4.1LG12g09050.1 (mRNA) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG12g09050.1
TypemRNA
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionvacuolar protein sorting-associated protein 2 homolog 1-like
LocationCp4.1LG12: 5929703 .. 5933438 (-)
Sequence length1694
RNA-Seq ExpressionCp4.1LG12g09050.1
SyntenyCp4.1LG12g09050.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AACAATGATACAATTCGAGAATAATGCTTTTACGTACCGATCGACCAGAAGAAAAGGTGGCTCCGCTCCTCCATGACGAGAGCCCACCGTGGCCGGTGACCTCCATCGACGGACTTTGAGTAAGGGGAGAGTTGCAGCGTAACGGAAGGCGAAGAGAAGAAGGAATAAGCTCGTTGAGATCGGAAATTGCACGTATCTTTTGGTTTTATCAATAGGCGGTTAAGTATGAGCTTCCTCTTTGGCAGCCGGAAAACTCCTGCAGGTCCATTATGTTCTTACCTTCTCTAATTGTGCATATTCTGGAGCTTTGTTCTTAATCATAGCGTGATTGCTTGTGATGAATTTCTAACTCTGGAGTCTAGTCGAATAAACGTATGTTTGATGTGGATGAAATTTTGCCTTTTACCTTCTCAATTTTGCATTTGTCAGAGCTTTGTTCTTGTGATAGCGTGATTTTTTGTGTGAATTTCTAAGTCTGGAGCTCAGTCCTGTAAACGTCATTTTGATGTGGATGAAATTTCTTTGACGGTAATTTCAGTACATCTAATTTTACTCATGATATAGAGGAATCATATTTTCCCATGCGTTGTGATTTGTATGAGTGAATTCGTGCGTTCTACTTCTATTACAATAGACGGCTCTAGATTTGTAATCTGTTTTGGTTTTCTGCGCAGAGCTTTTGCGGGAGAACAAGAGGATGCTTGATAAGTCCATAAGAGAAATTGAACGGGAAAGACAAGGCTTACAGTCTCAGGAAAAGAAGTTGATTGTCGAGATAAAGAAAAATGCTAAACAAGGACAGATGGTGAGTTGAGCAATTTAGAACTTGTTCTTTGACATCGACACATTTCAGTGACGGGGTTTCTATAGCTCTACGGGATGGAAGTGTGCTTCTACTTCTTTTCTTTTGTTTCTTTAGAAAGGGCGATGGATTCCTGGTTGGAACCATCAACTCCGCACAACTTCCTTGATTCCTTTGTTTTTGTTTTCTCCAGGGAGCAGTTAAAATAATGGCCAAAGATCTCATCAGGACACGACATCAGATTGAAAAATTTTATAAACTAAAATCACAGCTACAGGGCGTGGCTTTGAGAATTCAGGTATTCTATGTGCACCTTTCTCCGAAACTGATGTTACTTCTCATCTTTCATTTTCATCAGAGCGAGAACAATTACTGCTCATTTTTGTACAAATTTTTGGATTATAGTTACTGATTTGAAGTGATTGGACGCAGAGGTAGTTATTCTTTATAGATGTGCTCTAAAAGCCCAAAATGAAAGTTTAAGGTTATAACTATCTAGAGCTTGTTAAGCTTTCATTGTAGATGCATTTTCTTGGTTCAAATTCTAACTGAGGCGTGCTTATATCTGTGCTAAGAAAAGGAACATCTTGCAAACCATACAGTTCTCTTCCCGCATGATTAAAGAACAAACAACAAAATCCTTTAGTATTAGTTTAATTTCTCTTGCATCTTCTAATTTTTTTGTACAGTAAAAGGAAGGTTTGAAAAAAATTGGGATTTTGGGGCAAGAGTATTTTCTTGCTTCAAGTTTATATGCAAATTGGAATTTGAAGTAGTTTATTCAAATCACTTTTTAGAATAATGGATTTGATTCCATTAAATCTTATGTGCCTTTTTAGGGTTTGTTTCTAGATTGATTTTTCATAACAAAGTTGGAAAAACTATCTTTTCAGTTCTTTGTGATTTCAGAAACGTGGGAAGGTTGTTTTTAGTTTGTCTGTTTACTTTTCATGGTTTGTCTAATGTCGCATTCTGTATCACTGTCCATGAAAATACACTGTATAAACATAATTTTCCATTGTTTATATTTACGAAACAAGTGGATGTATCATTAGAATGTTTTCTATTTGAAGAAAACTGTTCATCACTGTAAGAGAAGCTTTCAGCATTAAACGATTAATTTTGGAAATAGAATTTTTCTTTATAAATTTTGGAAATGAACTCTTACTTTCTTTTTGAGACCATAAAGATATAAAGTTGTTTTATTTAATCAATTGACACGTACAATTTCTGCATTTGTGATTGTTCAAATTTTGCTCCAAATTGTTTGCTTTCTGCAGACGTTGAAATCAACTCAAGCTATGGGAGAAGCCATGAAAGGCGTTACTAAGGCGATGGGCCAAATGAATAGGCAGATGAACTTGCCGGCATTACAGAAGATAATGCAAGAGTTTGAGAGGCAGAATGAAAGGATGGAAATGACATCTGAAGTAATGGCAGACGTTCTTGATGATGCTTTAGAAGGAGATGAAGAAGAGGAGGAGACAGTCGAGCTTGTGAATCAGGTGCTTGATGAGATTGGAATTGATATCAACTCAGAGGTAAATGTGCTTGCACACATACAGATTTTTTTCATAATGGTTCATATCTTGTCATGGGAAAAGGTAATTTAAACGCAATCGTGATTGTTTTTTGTTCTCTCTCACATACAGCTTGTGAATGCACCCTCAGCTTCTGTGTCAGTTTCAACTGCTAATAATAGGGTTGCCCAAGCAGAAGCCGAAGATACTGGAATTGACAGTGATTTACAGGCTAGGCTGGATAACTTGAGAAAAATGTAGGTTTTTCTATTATGCGAAATTAATAACTCTCAGATAATAGACATAATAAATCATGTATTGTTTATAGTTGAACTCTATGTATTTGAATACTACATTGACAAGTTTGATCTCTTGTTTGGAGAAGAGAATGAATTTAGCACTTGGGATTAGGTAATTCTCTCTTTACAAATTTAAGCCTTCAAGGAAGACAAAGGTTTTGGATTGATAGTATAGATTTGCATGAGATTAACCATTCCACTTCTTTGTCAAGTTGTTCTAGAACTAGAAGCCTTGAAGCTTTCTGAGTGCATATCGAGGTCTTCTTATTTCGATATGCAGTTGAATTATTAAACCATGTTTTCTGGCCACGCTTCGCTCTTAATTACATGAGGAGAAAGCTCAATCTGGAGACTGTTAATGTCTACTACCTGAGAAATCACCGCCTCTGGAGTCCGACAAGCTACACGGTGGACTGGAGTCCGACAGAGTGGGCACTCTGAATGTGAAGAGAGCCATACGTCGAAACACGAAGCATGAAAGACATGGTTGCAGCTGGGAAGCTGCCGTATGCATTCCCCATCTTCAAAAGGTGACAAGCACAAAACACAATCACTTTCAGTATTAGTTTCTTCATTCTCATTTTCTTTCTTGTACGTGACAGTTGGGATCTGCATGATATGTGATACTTCATTCTTAATATTCTCAACCGACTCGGTCGTCCCGGGTGAATGGCAGCAGCAATTGAACCATCCTAGTATTGTGAGATGGAAGAATGTGAGGGATATAATCAGTGAGCAAATAATGGCAATGCCGGAGCAAATGAAGAAGACGGACATGATGAATTCAGATTAGGAGCTCTCAAGTGTCGTGCTGAACAATGGAAATCAGATAAAAGATGCTGCAGCTGTAACGAACAATGAACGGCAAATCTTATGGACGTATTAACAATTTTGGCAATTGTTCCAGGTACGAATCTTCATAAAACGACATAAAAATGTGAGTCATCTTTTTTGTTTCCTTATTTTTCTCCTGACATAGTTCATCAACTTTTGTGGGTTCCGAACTGCTGAACTTGAAATATGTGCTTTGTCCGCGTCAACCCTCATTGCCCATTCCGATTACGCTCATAATGTGGCTGCAGGCATGCGTTGAAGTTAAGGTAACTTCTTTCTTT

mRNA sequence

AACAATGATACAATTCGAGAATAATGCTTTTACGTACCGATCGACCAGAAGAAAAGGTGGCTCCGCTCCTCCATGACGAGAGCCCACCGTGGCCGGTGACCTCCATCGACGGACTTTGAGTAAGGGGAGAGTTGCAGCGTAACGGAAGGCGAAGAGAAGAAGGAATAAGCTCGTTGAGATCGGAAATTGCACGTATCTTTTGGTTTTATCAATAGGCGGTTAAGTATGAGCTTCCTCTTTGGCAGCCGGAAAACTCCTGCAGAGCTTTTGCGGGAGAACAAGAGGATGCTTGATAAGTCCATAAGAGAAATTGAACGGGAAAGACAAGGCTTACAGTCTCAGGAAAAGAAGTTGATTGTCGAGATAAAGAAAAATGCTAAACAAGGACAGATGGGAGCAGTTAAAATAATGGCCAAAGATCTCATCAGGACACGACATCAGATTGAAAAATTTTATAAACTAAAATCACAGCTACAGGGCGTGGCTTTGAGAATTCAGACGTTGAAATCAACTCAAGCTATGGGAGAAGCCATGAAAGGCGTTACTAAGGCGATGGGCCAAATGAATAGGCAGATGAACTTGCCGGCATTACAGAAGATAATGCAAGAGTTTGAGAGGCAGAATGAAAGGATGGAAATGACATCTGAAGTAATGGCAGACGTTCTTGATGATGCTTTAGAAGGAGATGAAGAAGAGGAGGAGACAGTCGAGCTTGTGAATCAGGTGCTTGATGAGATTGGAATTGATATCAACTCAGAGCTTGTGAATGCACCCTCAGCTTCTGTGTCAGTTTCAACTGCTAATAATAGGGTTGCCCAAGCAGAAGCCGAAGATACTGGAATTGACAGTGATTTACAGGCTAGGCTGGATAACTTGAGAAAAATGTAGGTTTTTCTATTATGCGAAATTAATAACTCTCAGATAATAGACATAATAAATCATGTATTGTTTATAGTTGAACTCTATGTATTTGAATACTACATTGACAAGTTTGATCTCTTGTTTGGAGAAGAGAATGAATTTAGCACTTGGGATTAGTTGTTCTAGAACTAGAAGCCTTGAAGCTTTCTGAGTGCATATCGAGGTCTTCTTATTTCGATATGCAGTTGAATTATTAAACCATGTTTTCTGGCCACGCTTCGCTCTTAATTACATGAGGAGAAAGCTCAATCTGGAGACTGTTAATGTCTACTACCTGAGAAATCACCGCCTCTGGAGTCCGACAAGCTACACGGTGGACTGGAGTCCGACAGAGTGGGCACTCTGAATGTGAAGAGAGCCATACGTCGAAACACGAAGCATGAAAGACATGGTTGCAGCTGGGAAGCTGCCGTATGCATTCCCCATCTTCAAAAGTTGGGATCTGCATGATATGTGATACTTCATTCTTAATATTCTCAACCGACTCGGTCGTCCCGGGTGAATGGCAGCAGCAATTGAACCATCCTAGTATTGTGAGATGGAAGAATGTGAGGGATATAATCAGTGAGCAAATAATGGCAATGCCGGAGCAAATGAAGAAGACGGACATGATGAATTCAGATTAGGAGCTCTCAAGTGTCGTGCTGAACAATGGAAATCAGATAAAAGATGCTGCAGCTGTAACGAACAATGAACGGCAAATCTTATGGACGTATTAACAATTTTGGCAATTGTTCCAGGCATGCGTTGAAGTTAAGGTAACTTCTTTCTTT

Coding sequence (CDS)

ATGAGCTTCCTCTTTGGCAGCCGGAAAACTCCTGCAGAGCTTTTGCGGGAGAACAAGAGGATGCTTGATAAGTCCATAAGAGAAATTGAACGGGAAAGACAAGGCTTACAGTCTCAGGAAAAGAAGTTGATTGTCGAGATAAAGAAAAATGCTAAACAAGGACAGATGGGAGCAGTTAAAATAATGGCCAAAGATCTCATCAGGACACGACATCAGATTGAAAAATTTTATAAACTAAAATCACAGCTACAGGGCGTGGCTTTGAGAATTCAGACGTTGAAATCAACTCAAGCTATGGGAGAAGCCATGAAAGGCGTTACTAAGGCGATGGGCCAAATGAATAGGCAGATGAACTTGCCGGCATTACAGAAGATAATGCAAGAGTTTGAGAGGCAGAATGAAAGGATGGAAATGACATCTGAAGTAATGGCAGACGTTCTTGATGATGCTTTAGAAGGAGATGAAGAAGAGGAGGAGACAGTCGAGCTTGTGAATCAGGTGCTTGATGAGATTGGAATTGATATCAACTCAGAGCTTGTGAATGCACCCTCAGCTTCTGTGTCAGTTTCAACTGCTAATAATAGGGTTGCCCAAGCAGAAGCCGAAGATACTGGAATTGACAGTGATTTACAGGCTAGGCTGGATAACTTGAGAAAAATGTAG

Protein sequence

MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVKIMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLPALQKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEETVELVNQVLDEIGIDINSELVNAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM
Homology
BLAST of Cp4.1LG12g09050.1 vs. ExPASy Swiss-Prot
Match: Q9SKI2 (Vacuolar protein sorting-associated protein 2 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=VPS2.1 PE=1 SV=2)

HSP 1 Score: 341.3 bits (874), Expect = 8.3e-93
Identity = 192/224 (85.71%), Postives = 206/224 (91.96%), Query Frame = 0

Query: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60
           M+ +FG RKTPAELLRENKRMLDKSIREIERERQGLQ+QEKKLI EIKK AKQGQMGAVK
Sbjct: 2   MNSIFGKRKTPAELLRENKRMLDKSIREIERERQGLQTQEKKLINEIKKTAKQGQMGAVK 61

Query: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120
           +MAKDLIRTRHQIEKFYKLKSQLQGV+LRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP
Sbjct: 62  VMAKDLIRTRHQIEKFYKLKSQLQGVSLRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 121

Query: 121 ALQKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEETVELVNQVLDEIGIDINSELV 180
           +LQKIMQEFERQNE+MEM SEVM D +DDALEGDEEEEET +LV+QVLDEIGIDIN ELV
Sbjct: 122 SLQKIMQEFERQNEKMEMVSEVMGDAIDDALEGDEEEEETEDLVSQVLDEIGIDINQELV 181

Query: 181 NAPSASVSVSTANNRVAQAE---AEDT-GIDSDLQARLDNLRKM 221
           NAPS +V+V  A N+V QAE   AED+ GIDSDLQARLDNLRKM
Sbjct: 182 NAPSGAVAVPAAKNKVVQAEATGAEDSGGIDSDLQARLDNLRKM 225

BLAST of Cp4.1LG12g09050.1 vs. ExPASy Swiss-Prot
Match: Q54DB1 (Charged multivesicular body protein 2a homolog 2 OS=Dictyostelium discoideum OX=44689 GN=chmp2a2 PE=3 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 1.9e-57
Identity = 123/191 (64.40%), Postives = 160/191 (83.77%), Query Frame = 0

Query: 3   FLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVKIM 62
           FLFG +KTP E+LREN+R L+KS+REI+RER  LQ+QEKK+I++IKK AKQGQM + KIM
Sbjct: 2   FLFGKQKTPKEVLRENQRNLNKSMREIDRERVALQNQEKKIILDIKKMAKQGQMNSAKIM 61

Query: 63  AKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLPAL 122
           AKDL+RTR+ I+KFY++K+QLQ V+LRIQTL+STQAM EAMKGVTKAM  MNRQMNLP  
Sbjct: 62  AKDLVRTRYHIQKFYEMKTQLQAVSLRIQTLQSTQAMAEAMKGVTKAMITMNRQMNLPQF 121

Query: 123 QKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEETVELVNQVLDEIGIDINSELVNA 182
            KIM EFE+Q+++M+M  E+M D +D  +E D EEE++ E++NQVLDEIGID+ S+LV+A
Sbjct: 122 TKIMMEFEQQSDKMDMKEEMMNDTMDQVMEQDGEEEQSQEILNQVLDEIGIDLASQLVDA 181

Query: 183 PSASVSVSTAN 194
           P+ +V  S A+
Sbjct: 182 PT-TVGTSVAS 191

BLAST of Cp4.1LG12g09050.1 vs. ExPASy Swiss-Prot
Match: Q6IP52 (Charged multivesicular body protein 2a OS=Xenopus laevis OX=8355 GN=chmp2a PE=2 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 3.3e-57
Identity = 121/219 (55.25%), Postives = 167/219 (76.26%), Query Frame = 0

Query: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60
           M FLFG RKTP ELLR+N+R L++++RE++RERQ L+ QEKK+I +IKK AKQGQM AVK
Sbjct: 1   MEFLFGRRKTPEELLRQNQRALNRAMRELDRERQKLEQQEKKIIADIKKMAKQGQMDAVK 60

Query: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120
           IMAKDL+RTR  ++KF  +++ +Q V+L+IQTLKS  +M +AMKGVTKAM  MNRQ+ LP
Sbjct: 61  IMAKDLVRTRRYVKKFIMMRANIQAVSLKIQTLKSNNSMAQAMKGVTKAMATMNRQLKLP 120

Query: 121 ALQKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEETVELVNQVLDEIGIDINSELV 180
            +QKIM EFE+Q+E M+M  E+M D +DDA+  +++EEE+  +V+QVLDE+G+ +  EL 
Sbjct: 121 QIQKIMMEFEKQSEIMDMKEEMMNDAIDDAMGDEDDEEESDAVVSQVLDELGLTLTDELS 180

Query: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRK 220
           N PS   S+S A  +  +A A     D+DL+ RL+NLR+
Sbjct: 181 NLPSTGGSLSVAGAKKGEATAALADADADLEERLNNLRR 219

BLAST of Cp4.1LG12g09050.1 vs. ExPASy Swiss-Prot
Match: Q6DFS6 (Charged multivesicular body protein 2a OS=Xenopus tropicalis OX=8364 GN=chmp2a PE=2 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 9.6e-57
Identity = 120/219 (54.79%), Postives = 166/219 (75.80%), Query Frame = 0

Query: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60
           M FLFG RKTP E+LR+N+R L+K++RE++RERQ L+ QEKK+I +IKK AKQGQM AVK
Sbjct: 1   MEFLFGRRKTPEEMLRQNQRALNKAMREMDRERQKLEQQEKKIIADIKKMAKQGQMDAVK 60

Query: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120
           IMAKDL+RTR  ++KF  +++ +Q V+L+IQTLKS  +M +AMKGVTKAM  MNRQ+ LP
Sbjct: 61  IMAKDLVRTRRYVKKFIMMRANIQAVSLKIQTLKSNNSMAQAMKGVTKAMATMNRQLKLP 120

Query: 121 ALQKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEETVELVNQVLDEIGIDINSELV 180
            +QKIM EFE+Q+E M+M  E+M D +DDA+  +++EEE+  +V+QVLDE+G+ +  EL 
Sbjct: 121 QIQKIMMEFEKQSEIMDMKEEMMNDAIDDAMGDEDDEEESDAVVSQVLDELGLTLTDELS 180

Query: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRK 220
           N PS   S+S A  +  +  A     D+DL+ RL+NLR+
Sbjct: 181 NLPSTGGSLSVAGAKKGEPSAALADADADLEERLNNLRR 219

BLAST of Cp4.1LG12g09050.1 vs. ExPASy Swiss-Prot
Match: O43633 (Charged multivesicular body protein 2a OS=Homo sapiens OX=9606 GN=CHMP2A PE=1 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 1.4e-55
Identity = 121/221 (54.75%), Postives = 165/221 (74.66%), Query Frame = 0

Query: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60
           M  LFG RKTP ELLR+N+R L++++RE++RERQ L++QEKK+I +IKK AKQGQM AV+
Sbjct: 1   MDLLFGRRKTPEELLRQNQRALNRAMRELDRERQKLETQEKKIIADIKKMAKQGQMDAVR 60

Query: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120
           IMAKDL+RTR  + KF  +++ +Q V+L+IQTLKS  +M +AMKGVTKAMG MNRQ+ LP
Sbjct: 61  IMAKDLVRTRRYVRKFVLMRANIQAVSLKIQTLKSNNSMAQAMKGVTKAMGTMNRQLKLP 120

Query: 121 ALQKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEETVELVNQVLDEIGIDINSELV 180
            +QKIM EFERQ E M+M  E+M D +DDA+  +E+EEE+  +V+QVLDE+G+ +  EL 
Sbjct: 121 QIQKIMMEFERQAEIMDMKEEMMNDAIDDAMGDEEDEEESDAVVSQVLDELGLSLTDELS 180

Query: 181 NAPS--ASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRK 220
           N PS   S+SV+    +   A +     D+DL+ RL NLR+
Sbjct: 181 NLPSTGGSLSVAAGGKKAEAAASALADADADLEERLKNLRR 221

BLAST of Cp4.1LG12g09050.1 vs. NCBI nr
Match: XP_023547513.1 (vacuolar protein sorting-associated protein 2 homolog 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 390 bits (1003), Expect = 3.85e-136
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 0

Query: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60
           MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK
Sbjct: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60

Query: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120
           IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP
Sbjct: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120

Query: 121 ALQKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEETVELVNQVLDEIGIDINSELV 180
           ALQKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEETVELVNQVLDEIGIDINSELV
Sbjct: 121 ALQKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEETVELVNQVLDEIGIDINSELV 180

Query: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM 220
           NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM
Sbjct: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM 220

BLAST of Cp4.1LG12g09050.1 vs. NCBI nr
Match: XP_022954248.1 (vacuolar protein sorting-associated protein 2 homolog 1-like [Cucurbita moschata] >XP_022992389.1 vacuolar protein sorting-associated protein 2 homolog 1-like [Cucurbita maxima] >KAG7013995.1 Vacuolar protein sorting-associated protein 2-like 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 388 bits (996), Expect = 4.49e-135
Identity = 219/220 (99.55%), Postives = 219/220 (99.55%), Query Frame = 0

Query: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60
           MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK
Sbjct: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60

Query: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120
           IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP
Sbjct: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120

Query: 121 ALQKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEETVELVNQVLDEIGIDINSELV 180
           ALQKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEET ELVNQVLDEIGIDINSELV
Sbjct: 121 ALQKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEETDELVNQVLDEIGIDINSELV 180

Query: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM 220
           NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM
Sbjct: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM 220

BLAST of Cp4.1LG12g09050.1 vs. NCBI nr
Match: XP_008462317.1 (PREDICTED: vacuolar protein sorting-associated protein 2 homolog 1-like [Cucumis melo] >XP_038897571.1 vacuolar protein sorting-associated protein 2 homolog 1-like [Benincasa hispida])

HSP 1 Score: 386 bits (992), Expect = 1.83e-134
Identity = 218/220 (99.09%), Postives = 218/220 (99.09%), Query Frame = 0

Query: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60
           MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK
Sbjct: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60

Query: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120
           IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP
Sbjct: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120

Query: 121 ALQKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEETVELVNQVLDEIGIDINSELV 180
           ALQKIMQEFERQNERMEMTSEVMAD LDDALEGDEEEEET ELVNQVLDEIGIDINSELV
Sbjct: 121 ALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEEETDELVNQVLDEIGIDINSELV 180

Query: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM 220
           NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM
Sbjct: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM 220

BLAST of Cp4.1LG12g09050.1 vs. NCBI nr
Match: KAG6575451.1 (Vacuolar protein sorting-associated protein 2-like 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 386 bits (992), Expect = 3.12e-134
Identity = 218/220 (99.09%), Postives = 219/220 (99.55%), Query Frame = 0

Query: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60
           MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK
Sbjct: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60

Query: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120
           IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP
Sbjct: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120

Query: 121 ALQKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEETVELVNQVLDEIGIDINSELV 180
           ALQKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEET ELVNQVLDEIGIDINSELV
Sbjct: 121 ALQKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEETDELVNQVLDEIGIDINSELV 180

Query: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM 220
           NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRK+
Sbjct: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKI 220

BLAST of Cp4.1LG12g09050.1 vs. NCBI nr
Match: XP_004141696.1 (vacuolar protein sorting-associated protein 2 homolog 1 [Cucumis sativus] >KGN45498.1 hypothetical protein Csa_016370 [Cucumis sativus])

HSP 1 Score: 383 bits (984), Expect = 3.03e-133
Identity = 216/220 (98.18%), Postives = 218/220 (99.09%), Query Frame = 0

Query: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60
           MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK
Sbjct: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60

Query: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120
           IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMG+AMKGVTKAMGQMNRQMNLP
Sbjct: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGDAMKGVTKAMGQMNRQMNLP 120

Query: 121 ALQKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEETVELVNQVLDEIGIDINSELV 180
           ALQKIMQEFERQNERMEMTSEVMAD LDDALEGDEEEEET ELV+QVLDEIGIDINSELV
Sbjct: 121 ALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEEETDELVSQVLDEIGIDINSELV 180

Query: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM 220
           NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM
Sbjct: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM 220

BLAST of Cp4.1LG12g09050.1 vs. ExPASy TrEMBL
Match: A0A6J1JXE8 (vacuolar protein sorting-associated protein 2 homolog 1-like OS=Cucurbita maxima OX=3661 GN=LOC111488711 PE=4 SV=1)

HSP 1 Score: 388 bits (996), Expect = 2.18e-135
Identity = 219/220 (99.55%), Postives = 219/220 (99.55%), Query Frame = 0

Query: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60
           MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK
Sbjct: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60

Query: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120
           IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP
Sbjct: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120

Query: 121 ALQKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEETVELVNQVLDEIGIDINSELV 180
           ALQKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEET ELVNQVLDEIGIDINSELV
Sbjct: 121 ALQKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEETDELVNQVLDEIGIDINSELV 180

Query: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM 220
           NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM
Sbjct: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM 220

BLAST of Cp4.1LG12g09050.1 vs. ExPASy TrEMBL
Match: A0A6J1GQK3 (vacuolar protein sorting-associated protein 2 homolog 1-like OS=Cucurbita moschata OX=3662 GN=LOC111456560 PE=4 SV=1)

HSP 1 Score: 388 bits (996), Expect = 2.18e-135
Identity = 219/220 (99.55%), Postives = 219/220 (99.55%), Query Frame = 0

Query: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60
           MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK
Sbjct: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60

Query: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120
           IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP
Sbjct: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120

Query: 121 ALQKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEETVELVNQVLDEIGIDINSELV 180
           ALQKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEET ELVNQVLDEIGIDINSELV
Sbjct: 121 ALQKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEETDELVNQVLDEIGIDINSELV 180

Query: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM 220
           NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM
Sbjct: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM 220

BLAST of Cp4.1LG12g09050.1 vs. ExPASy TrEMBL
Match: A0A1S3CGM4 (vacuolar protein sorting-associated protein 2 homolog 1-like OS=Cucumis melo OX=3656 GN=LOC103500700 PE=4 SV=1)

HSP 1 Score: 386 bits (992), Expect = 8.86e-135
Identity = 218/220 (99.09%), Postives = 218/220 (99.09%), Query Frame = 0

Query: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60
           MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK
Sbjct: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60

Query: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120
           IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP
Sbjct: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120

Query: 121 ALQKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEETVELVNQVLDEIGIDINSELV 180
           ALQKIMQEFERQNERMEMTSEVMAD LDDALEGDEEEEET ELVNQVLDEIGIDINSELV
Sbjct: 121 ALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEEETDELVNQVLDEIGIDINSELV 180

Query: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM 220
           NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM
Sbjct: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM 220

BLAST of Cp4.1LG12g09050.1 vs. ExPASy TrEMBL
Match: A0A0A0K7U7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G450570 PE=4 SV=1)

HSP 1 Score: 383 bits (984), Expect = 1.47e-133
Identity = 216/220 (98.18%), Postives = 218/220 (99.09%), Query Frame = 0

Query: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60
           MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK
Sbjct: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60

Query: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120
           IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMG+AMKGVTKAMGQMNRQMNLP
Sbjct: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGDAMKGVTKAMGQMNRQMNLP 120

Query: 121 ALQKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEETVELVNQVLDEIGIDINSELV 180
           ALQKIMQEFERQNERMEMTSEVMAD LDDALEGDEEEEET ELV+QVLDEIGIDINSELV
Sbjct: 121 ALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEEETDELVSQVLDEIGIDINSELV 180

Query: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM 220
           NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM
Sbjct: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM 220

BLAST of Cp4.1LG12g09050.1 vs. ExPASy TrEMBL
Match: A0A6J1KC98 (vacuolar protein sorting-associated protein 2 homolog 1-like OS=Cucurbita maxima OX=3661 GN=LOC111494308 PE=4 SV=1)

HSP 1 Score: 380 bits (976), Expect = 2.43e-132
Identity = 213/220 (96.82%), Postives = 217/220 (98.64%), Query Frame = 0

Query: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60
           MSFLFGSRKTPAE+LRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK
Sbjct: 1   MSFLFGSRKTPAEILRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60

Query: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120
           IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP
Sbjct: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120

Query: 121 ALQKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEETVELVNQVLDEIGIDINSELV 180
           ALQKIMQEFERQNE+MEMTSE+MAD LDDA EGDEEEEET ELV+QVLDEIGIDINSELV
Sbjct: 121 ALQKIMQEFERQNEKMEMTSEMMADALDDAFEGDEEEEETEELVSQVLDEIGIDINSELV 180

Query: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM 220
           NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM
Sbjct: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM 220

BLAST of Cp4.1LG12g09050.1 vs. TAIR 10
Match: AT2G06530.1 (SNF7 family protein )

HSP 1 Score: 341.3 bits (874), Expect = 5.9e-94
Identity = 192/224 (85.71%), Postives = 206/224 (91.96%), Query Frame = 0

Query: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60
           M+ +FG RKTPAELLRENKRMLDKSIREIERERQGLQ+QEKKLI EIKK AKQGQMGAVK
Sbjct: 2   MNSIFGKRKTPAELLRENKRMLDKSIREIERERQGLQTQEKKLINEIKKTAKQGQMGAVK 61

Query: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120
           +MAKDLIRTRHQIEKFYKLKSQLQGV+LRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP
Sbjct: 62  VMAKDLIRTRHQIEKFYKLKSQLQGVSLRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 121

Query: 121 ALQKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEETVELVNQVLDEIGIDINSELV 180
           +LQKIMQEFERQNE+MEM SEVM D +DDALEGDEEEEET +LV+QVLDEIGIDIN ELV
Sbjct: 122 SLQKIMQEFERQNEKMEMVSEVMGDAIDDALEGDEEEEETEDLVSQVLDEIGIDINQELV 181

Query: 181 NAPSASVSVSTANNRVAQAE---AEDT-GIDSDLQARLDNLRKM 221
           NAPS +V+V  A N+V QAE   AED+ GIDSDLQARLDNLRKM
Sbjct: 182 NAPSGAVAVPAAKNKVVQAEATGAEDSGGIDSDLQARLDNLRKM 225

BLAST of Cp4.1LG12g09050.1 vs. TAIR 10
Match: AT1G03950.1 (vacuolar protein sorting-associated protein 2.3 )

HSP 1 Score: 153.3 bits (386), Expect = 2.3e-37
Identity = 93/215 (43.26%), Postives = 141/215 (65.58%), Query Frame = 0

Query: 4   LFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVKIMA 63
           +F  +  P E+LRE+KR + ++ R IE+E   LQS+EKKL++EIK+ AK G  GA KI+A
Sbjct: 3   IFTKKPNPREVLRESKREMTQATRGIEKEIGSLQSEEKKLVLEIKRTAKSGNEGATKILA 62

Query: 64  KDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLPALQ 123
           + LIR R QI      ++Q++G+A   Q + +  ++   M+G TKAM  M++ M+     
Sbjct: 63  RQLIRLRQQIANLQGSRAQMRGIATHTQAMHAHTSVAAGMQGATKAMAAMSKNMDPAKQA 122

Query: 124 KIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEETVELVNQVLDEIGIDINSELVNAP 183
           K+M+EF++Q+ +M+MT+E+M+D +DDAL+ DE E+ET +L NQVLDEIGIDI S+L +AP
Sbjct: 123 KVMREFQKQSAQMDMTTEMMSDSIDDALDNDEAEDETEDLTNQVLDEIGIDIASQLSSAP 182

Query: 184 SASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLR 219
              +    A       +   +GID +L+ RL  LR
Sbjct: 183 KGKIGGKKAE------DVGSSGID-ELEKRLAALR 210

BLAST of Cp4.1LG12g09050.1 vs. TAIR 10
Match: AT5G44560.1 (SNF7 family protein )

HSP 1 Score: 141.7 bits (356), Expect = 6.8e-34
Identity = 86/221 (38.91%), Postives = 139/221 (62.90%), Query Frame = 0

Query: 4   LFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVKIMA 63
           +F  + TP + LR +KR +  + R IERE   LQ +EK+L+ EIKK AK G   A KI+A
Sbjct: 3   IFKKKTTPKDALRTSKREMAVATRGIEREITSLQLEEKRLVAEIKKTAKTGNEAATKILA 62

Query: 64  KDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLPALQ 123
           + L+R R QI      ++Q++GV    Q L ++ ++   MKG TKAM  MN+QM      
Sbjct: 63  RQLVRLRQQITNLQGSRAQIRGVTTHTQALYASTSISSGMKGATKAMVAMNKQMAPTKQA 122

Query: 124 KIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEETVELVNQVLDEIGIDINSELVNAP 183
           K++++F++Q+ +++MT E+M++ +D+ L+ DE EEET +L NQVLDEIG+ + S+L +AP
Sbjct: 123 KVIKDFQKQSAQLDMTIEMMSEAIDETLDKDEAEEETEDLTNQVLDEIGVGVASQLSSAP 182

Query: 184 SASVSVST----ANNRVAQAEAEDTGIDSDLQARLDNLRKM 221
              ++  T    A+       +E + +D +L+ RL +LR++
Sbjct: 183 KGRIATKTAAPPASTAATNKNSESSEVD-ELEKRLASLRRI 222

BLAST of Cp4.1LG12g09050.1 vs. TAIR 10
Match: AT5G44560.2 (SNF7 family protein )

HSP 1 Score: 129.4 bits (324), Expect = 3.5e-30
Identity = 79/198 (39.90%), Postives = 126/198 (63.64%), Query Frame = 0

Query: 27  REIERERQGLQSQEKKLIVEIKKNAKQGQMGAVKIMAKDLIRTRHQIEKFYKLKSQLQGV 86
           R IERE   LQ +EK+L+ EIKK AK G   A KI+A+ L+R R QI      ++Q++GV
Sbjct: 6   RGIEREITSLQLEEKRLVAEIKKTAKTGNEAATKILARQLVRLRQQITNLQGSRAQIRGV 65

Query: 87  ALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLPALQKIMQEFERQNERMEMTSEVMADV 146
               Q L ++ ++   MKG TKAM  MN+QM      K++++F++Q+ +++MT E+M++ 
Sbjct: 66  TTHTQALYASTSISSGMKGATKAMVAMNKQMAPTKQAKVIKDFQKQSAQLDMTIEMMSEA 125

Query: 147 LDDALEGDEEEEETVELVNQVLDEIGIDINSELVNAPSASVSVST----ANNRVAQAEAE 206
           +D+ L+ DE EEET +L NQVLDEIG+ + S+L +AP   ++  T    A+       +E
Sbjct: 126 IDETLDKDEAEEETEDLTNQVLDEIGVGVASQLSSAPKGRIATKTAAPPASTAATNKNSE 185

Query: 207 DTGIDSDLQARLDNLRKM 221
            + +D +L+ RL +LR++
Sbjct: 186 SSEVD-ELEKRLASLRRI 202

BLAST of Cp4.1LG12g09050.1 vs. TAIR 10
Match: AT5G22950.1 (SNF7 family protein )

HSP 1 Score: 74.3 bits (181), Expect = 1.3e-13
Identity = 56/217 (25.81%), Postives = 118/217 (54.38%), Query Frame = 0

Query: 11  PAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVKIMAKDLIRTR 70
           P +LLR+ +R L +  R IER+ + +Q +E+ +   IK+ AK+  M + K +AK+++ +R
Sbjct: 14  PKQLLRDWQRKLRQECRNIERQIRDIQKEERNVQKAIKEAAKRNDMVSAKALAKEIVSSR 73

Query: 71  HQIEKFYKLKSQLQGVALRI-QTLKSTQAMGEAMKGVTKAMGQMNRQMNLPALQKIMQEF 130
             + + Y+ K+Q+  +++ + +++   + +G   K   + M  +N  M  P +   MQEF
Sbjct: 74  RTVNRLYENKAQMNSISMHLGESVAIARTVGHLSKS-AEVMKLVNNLMKAPQMAATMQEF 133

Query: 131 ERQNERMEMTSEVMADVLDDALEGDEEEEETVELVNQVLD--------EIGIDINSELVN 190
            ++  +  +  E + + +D+AL+ ++ EEE  E V++VL         E+ + +  E + 
Sbjct: 134 SKEMTKAGVIEEFVNEAIDNALDSEDMEEEIDEEVDKVLTAIAGETAAELPVAVRKERIK 193

Query: 191 APSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLR 219
            P+   S S     VA+   ++  ++ +++ARL  +R
Sbjct: 194 VPAQKASTSREEEAVAEGVDDEEELE-EIRARLAKVR 228

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SKI28.3e-9385.71Vacuolar protein sorting-associated protein 2 homolog 1 OS=Arabidopsis thaliana ... [more]
Q54DB11.9e-5764.40Charged multivesicular body protein 2a homolog 2 OS=Dictyostelium discoideum OX=... [more]
Q6IP523.3e-5755.25Charged multivesicular body protein 2a OS=Xenopus laevis OX=8355 GN=chmp2a PE=2 ... [more]
Q6DFS69.6e-5754.79Charged multivesicular body protein 2a OS=Xenopus tropicalis OX=8364 GN=chmp2a P... [more]
O436331.4e-5554.75Charged multivesicular body protein 2a OS=Homo sapiens OX=9606 GN=CHMP2A PE=1 SV... [more]
Match NameE-valueIdentityDescription
XP_023547513.13.85e-136100.00vacuolar protein sorting-associated protein 2 homolog 1-like [Cucurbita pepo sub... [more]
XP_022954248.14.49e-13599.55vacuolar protein sorting-associated protein 2 homolog 1-like [Cucurbita moschata... [more]
XP_008462317.11.83e-13499.09PREDICTED: vacuolar protein sorting-associated protein 2 homolog 1-like [Cucumis... [more]
KAG6575451.13.12e-13499.09Vacuolar protein sorting-associated protein 2-like 1, partial [Cucurbita argyros... [more]
XP_004141696.13.03e-13398.18vacuolar protein sorting-associated protein 2 homolog 1 [Cucumis sativus] >KGN45... [more]
Match NameE-valueIdentityDescription
A0A6J1JXE82.18e-13599.55vacuolar protein sorting-associated protein 2 homolog 1-like OS=Cucurbita maxima... [more]
A0A6J1GQK32.18e-13599.55vacuolar protein sorting-associated protein 2 homolog 1-like OS=Cucurbita moscha... [more]
A0A1S3CGM48.86e-13599.09vacuolar protein sorting-associated protein 2 homolog 1-like OS=Cucumis melo OX=... [more]
A0A0A0K7U71.47e-13398.18Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G450570 PE=4 SV=1[more]
A0A6J1KC982.43e-13296.82vacuolar protein sorting-associated protein 2 homolog 1-like OS=Cucurbita maxima... [more]
Match NameE-valueIdentityDescription
AT2G06530.15.9e-9485.71SNF7 family protein [more]
AT1G03950.12.3e-3743.26vacuolar protein sorting-associated protein 2.3 [more]
AT5G44560.16.8e-3438.91SNF7 family protein [more]
AT5G44560.23.5e-3039.90SNF7 family protein [more]
AT5G22950.11.3e-1325.81SNF7 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 119..139
NoneNo IPR availableCOILSCoilCoilcoord: 12..46
NoneNo IPR availableGENE3D6.10.140.1230coord: 6..181
e-value: 1.1E-68
score: 232.6
NoneNo IPR availablePANTHERPTHR10476CHARGED MULTIVESICULAR BODY PROTEINcoord: 1..220
NoneNo IPR availablePANTHERPTHR10476:SF44VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 2 HOMOLOG 1-LIKEcoord: 1..220
IPR005024Snf7 familyPFAMPF03357Snf7coord: 17..186
e-value: 2.6E-43
score: 147.6

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cp4.1LG12g09050Cp4.1LG12g09050gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG12g09050.1:exon:008Cp4.1LG12g09050.1:exon:008exon
Cp4.1LG12g09050.1:exon:007Cp4.1LG12g09050.1:exon:007exon
Cp4.1LG12g09050.1:exon:006Cp4.1LG12g09050.1:exon:006exon
Cp4.1LG12g09050.1:exon:005Cp4.1LG12g09050.1:exon:005exon
Cp4.1LG12g09050.1:exon:004Cp4.1LG12g09050.1:exon:004exon
Cp4.1LG12g09050.1:exon:003Cp4.1LG12g09050.1:exon:003exon
Cp4.1LG12g09050.1:exon:002Cp4.1LG12g09050.1:exon:002exon
Cp4.1LG12g09050.1:exon:001Cp4.1LG12g09050.1:exon:001exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG12g09050.1:three_prime_utr:004Cp4.1LG12g09050.1:three_prime_utr:004three_prime_UTR
Cp4.1LG12g09050.1:three_prime_utr:003Cp4.1LG12g09050.1:three_prime_utr:003three_prime_UTR
Cp4.1LG12g09050.1:three_prime_utr:002Cp4.1LG12g09050.1:three_prime_utr:002three_prime_UTR
Cp4.1LG12g09050.1:three_prime_utr:001Cp4.1LG12g09050.1:three_prime_utr:001three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG12g09050.1:cds:005Cp4.1LG12g09050.1:cds:005CDS
Cp4.1LG12g09050.1:cds:004Cp4.1LG12g09050.1:cds:004CDS
Cp4.1LG12g09050.1:cds:003Cp4.1LG12g09050.1:cds:003CDS
Cp4.1LG12g09050.1:cds:002Cp4.1LG12g09050.1:cds:002CDS
Cp4.1LG12g09050.1:cds:001Cp4.1LG12g09050.1:cds:001CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG12g09050.1:five_prime_utr:001Cp4.1LG12g09050.1:five_prime_utr:001five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cp4.1LG12g09050.1Cp4.1LG12g09050.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007034 vacuolar transport
biological_process GO:0032509 endosome transport via multivesicular body sorting pathway
biological_process GO:0045324 late endosome to vacuole transport
biological_process GO:0015031 protein transport
cellular_component GO:0000815 ESCRT III complex
cellular_component GO:0005771 multivesicular body