Cp4.1LG11g10410.1 (mRNA) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG11g10410.1
Type	mRNA
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	calmodulin-like protein 7
Location	Cp4.1LG11: 8821964 .. 8822626 (+)
Sequence length	663
RNA-Seq Expression	Cp4.1LG11g10410.1
Synteny	Cp4.1LG11g10410.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAATCCGTGATGAACCTTCTGTTCTTGGCGGTTTTGTTCGTTGCTGGGTTTGTCAATTTCCTCATGTATTTCCCCACCAAGAGATTCACCGCGTGGTTCCAATCTATAAAACCCTCTCCCCAATCTGTCCATTTCAAACCCACCGCCGTTCCTCCGCTGTCTCCGGCGCCGGAAAAGAAGAAATCAGAGATGAAGAAGGTGTTTGGGACATTTGACAAGAACAACGATGGGTTCATAACGAAGAAGGAGCTGATGGAATCGCTGAAGAACATGAGTATGATGATTACAGAGAGGGAAGCAGAGGAAATGGTGAAAGGGGTGGATGAAAATGGAGATGGGTTGATTGATTTTGAGGAGTTTTGTGTTTTGGGGGAGAAATTAGTGATGGGTTTTGAAGTAAAACAGAGGAGGGTTGAAGATGAAGATGAAGATGAAGATGAACTGAGAGAGGCTTTTGGAGTGTTTGATAAGGACAAAGATGGAGTGATATCAGTGGAGGAATTGGGCTTGGTGCTGTCTTGTTTGGGGATGAATGAAGGGAAGAAACTTGAGAATTGTAAGGAAATGATCAAGAAAGTGGATTTGGATGGGGATGGAATGGTGAATTTTGATGAGTTTAAGAGGATGATGAGAAGTGGAACAACTCTGGTTTTCAATTGA

mRNA sequence

ATGGAATCCGTGATGAACCTTCTGTTCTTGGCGGTTTTGTTCGTTGCTGGGTTTGTCAATTTCCTCATGTATTTCCCCACCAAGAGATTCACCGCGTGGTTCCAATCTATAAAACCCTCTCCCCAATCTGTCCATTTCAAACCCACCGCCGTTCCTCCGCTGTCTCCGGCGCCGGAAAAGAAGAAATCAGAGATGAAGAAGGTGTTTGGGACATTTGACAAGAACAACGATGGGTTCATAACGAAGAAGGAGCTGATGGAATCGCTGAAGAACATGAGTATGATGATTACAGAGAGGGAAGCAGAGGAAATGGTGAAAGGGGTGGATGAAAATGGAGATGGGTTGATTGATTTTGAGGAGTTTTGTGTTTTGGGGGAGAAATTAGTGATGGGTTTTGAAGTAAAACAGAGGAGGGTTGAAGATGAAGATGAAGATGAAGATGAACTGAGAGAGGCTTTTGGAGTGTTTGATAAGGACAAAGATGGAGTGATATCAGTGGAGGAATTGGGCTTGGTGCTGTCTTGTTTGGGGATGAATGAAGGGAAGAAACTTGAGAATTGTAAGGAAATGATCAAGAAAGTGGATTTGGATGGGGATGGAATGGTGAATTTTGATGAGTTTAAGAGGATGATGAGAAGTGGAACAACTCTGGTTTTCAATTGA

Coding sequence (CDS)

ATGGAATCCGTGATGAACCTTCTGTTCTTGGCGGTTTTGTTCGTTGCTGGGTTTGTCAATTTCCTCATGTATTTCCCCACCAAGAGATTCACCGCGTGGTTCCAATCTATAAAACCCTCTCCCCAATCTGTCCATTTCAAACCCACCGCCGTTCCTCCGCTGTCTCCGGCGCCGGAAAAGAAGAAATCAGAGATGAAGAAGGTGTTTGGGACATTTGACAAGAACAACGATGGGTTCATAACGAAGAAGGAGCTGATGGAATCGCTGAAGAACATGAGTATGATGATTACAGAGAGGGAAGCAGAGGAAATGGTGAAAGGGGTGGATGAAAATGGAGATGGGTTGATTGATTTTGAGGAGTTTTGTGTTTTGGGGGAGAAATTAGTGATGGGTTTTGAAGTAAAACAGAGGAGGGTTGAAGATGAAGATGAAGATGAAGATGAACTGAGAGAGGCTTTTGGAGTGTTTGATAAGGACAAAGATGGAGTGATATCAGTGGAGGAATTGGGCTTGGTGCTGTCTTGTTTGGGGATGAATGAAGGGAAGAAACTTGAGAATTGTAAGGAAATGATCAAGAAAGTGGATTTGGATGGGGATGGAATGGTGAATTTTGATGAGTTTAAGAGGATGATGAGAAGTGGAACAACTCTGGTTTTCAATTGA

Protein sequence

MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEKKKSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEFCVLGEKLVMGFEVKQRRVEDEDEDEDELREAFGVFDKDKDGVISVEELGLVLSCLGMNEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN

Homology

[Close]

BLAST of Cp4.1LG11g10410.1 vs. ExPASy Swiss-Prot
Match: Q9LNE7 (Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 3.0e-34
Identity = 72/152 (47.37%), Postives = 112/152 (73.68%), Query Frame = 0


Query: 63  SEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEFC 122
           +E+K+VF  FDKN DG IT KEL E+L+++ + I ++E  +M++ +D NGDG +D +EF 
Sbjct: 4   TELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEF- 63

Query: 123 VLGEKLVMGFEVKQRRVEDEDEDEDELREAFGVFDKDKDGVISVEELGLVLSCLGMNEGK 182
                     E+ +  +++EDE+E++++EAF VFD++ DG I+V+EL  VLS LG+ +GK
Sbjct: 64  ---------GELYKTIMDEEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGK 123

Query: 183 KLENCKEMIKKVDLDGDGMVNFDEFKRMMRSG 215
            L++CK+MIKKVD+DGDG VN+ EF++MM+ G
Sbjct: 124 TLDDCKKMIKKVDVDGDGRVNYKEFRQMMKGG 145

[Close]

BLAST of Cp4.1LG11g10410.1 vs. ExPASy Swiss-Prot
Match: Q9SRR7 (Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 8.7e-34
Identity = 74/153 (48.37%), Postives = 111/153 (72.55%), Query Frame = 0


Query: 62  KSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEF 121
           ++E+ ++F  FD+N DG ITK+EL +SL+N+ + I +++  +M++ +D NGDG +D EEF
Sbjct: 3   QAELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEF 62

Query: 122 CVLGEKLVMGFEVKQRRVEDEDEDEDELREAFGVFDKDKDGVISVEELGLVLSCLGMNEG 181
             L + +          +E+ DE+ED +REAF VFD+++DG I+VEEL  VL+ LG+ +G
Sbjct: 63  GGLYQTI----------MEERDEEED-MREAFNVFDQNRDGFITVEELRSVLASLGLKQG 122

Query: 182 KKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSG 215
           + LE+CK MI KVD+DGDGMVNF EFK+MM+ G
Sbjct: 123 RTLEDCKRMISKVDVDGDGMVNFKEFKQMMKGG 144

[Close]

BLAST of Cp4.1LG11g10410.1 vs. ExPASy Swiss-Prot
Match: Q9SU00 (Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 9.6e-33
Identity = 73/153 (47.71%), Postives = 105/153 (68.63%), Query Frame = 0


Query: 62  KSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEF 121
           + E+ +VF  FDKN DG I K EL +  K++ +M+ E E  EM+  +D NGDG +D +EF
Sbjct: 3   RGELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEF 62

Query: 122 CVLGEKLVMGFEVKQRRVEDEDEDEDELREAFGVFDKDKDGVISVEELGLVLSCLGMNEG 181
             L +++V           +E E+E+++REAF VFD++ DG I+ EEL  VL+ +G+ +G
Sbjct: 63  GSLYQEMV-----------EEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQG 122

Query: 182 KKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSG 215
           + LE+CK+MI KVD+DGDGMVNF EFK+MMR G
Sbjct: 123 RTLEDCKKMISKVDVDGDGMVNFKEFKQMMRGG 144

[Close]

BLAST of Cp4.1LG11g10410.1 vs. ExPASy Swiss-Prot
Match: O22845 (Calmodulin-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=CML5 PE=2 SV=2)

HSP 1 Score: 140.6 bits (353), Expect = 2.1e-32
Identity = 79/190 (41.58%), Postives = 122/190 (64.21%), Query Frame = 0


Query: 32  AWF-----QSIKPSPQSVHFKPTAVPPLSPAPEKK--KSEMKKVFGTFDKNNDGFITKKE 91
           +WF     ++  PSP ++   P++    S AP K+   SE+K+VF  FDKN DG ITK+E
Sbjct: 30  SWFDKTLHKNSPPSPSTMLPSPSS----SSAPTKRIDPSELKRVFQMFDKNGDGRITKEE 89

Query: 92  LMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEFCVLGEKLVMGFEVKQRRVEDEDE 151
           L +SL+N+ + I +++  +M+  +D NGDG +D +EF  L   +V           D + 
Sbjct: 90  LNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDIDEFESLYSSIV------DEHHNDGET 149

Query: 152 DEDELREAFGVFDKDKDGVISVEELGLVLSCLGMNEGKKLENCKEMIKKVDLDGDGMVNF 211
           +E+++++AF VFD+D DG I+VEEL  V++ LG+ +GK L+ CK+MI +VD DGDG VN+
Sbjct: 150 EEEDMKDAFNVFDQDGDGFITVEELKSVMASLGLKQGKTLDGCKKMIMQVDADGDGRVNY 209

Query: 212 DEFKRMMRSG 215
            EF +MM+ G
Sbjct: 210 KEFLQMMKGG 209

[Close]

BLAST of Cp4.1LG11g10410.1 vs. ExPASy Swiss-Prot
Match: Q9ZR02 (Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 3.7e-32
Identity = 75/153 (49.02%), Postives = 106/153 (69.28%), Query Frame = 0


Query: 63  SEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEFC 122
           +E+ +VF  FDK+ DG IT KEL ES KN+ ++I E E  ++++ +D NGDG +D EEF 
Sbjct: 4   TELNRVFQMFDKDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIEEF- 63

Query: 123 VLGEKLVMGFEVKQRRVEDEDE-DEDELREAFGVFDKDKDGVISVEELGLVLSCLGMNEG 182
                   G   K   VEDEDE  E++++EAF VFD++ DG I+V+EL  VLS LG+ +G
Sbjct: 64  --------GELYKTIMVEDEDEVGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQG 123

Query: 183 KKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSG 215
           K LE C++MI +VD+DGDG VN+ EF++MM+ G
Sbjct: 124 KTLEECRKMIMQVDVDGDGRVNYMEFRQMMKKG 147

[Close]

BLAST of Cp4.1LG11g10410.1 vs. NCBI nr
Match: XP_023545557.1 (calmodulin-like protein 7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 426 bits (1095), Expect = 3.64e-150
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 0


Query: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEK 60
           MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEK
Sbjct: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEK 60

Query: 61  KKSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 120
           KKSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE
Sbjct: 61  KKSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 120

Query: 121 FCVLGEKLVMGFEVKQRRVEDEDEDEDELREAFGVFDKDKDGVISVEELGLVLSCLGMNE 180
           FCVLGEKLVMGFEVKQRRVEDEDEDEDELREAFGVFDKDKDGVISVEELGLVLSCLGMNE
Sbjct: 121 FCVLGEKLVMGFEVKQRRVEDEDEDEDELREAFGVFDKDKDGVISVEELGLVLSCLGMNE 180

Query: 181 GKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN 220
           GKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN
Sbjct: 181 GKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN 220

[Close]

BLAST of Cp4.1LG11g10410.1 vs. NCBI nr
Match: XP_022946838.1 (calmodulin-like protein 7 [Cucurbita moschata])

HSP 1 Score: 416 bits (1070), Expect = 2.54e-146
Identity = 218/222 (98.20%), Postives = 218/222 (98.20%), Query Frame = 0


Query: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEK 60
           MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEK
Sbjct: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEK 60

Query: 61  KKSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 120
           KKSEMKKVFGTFDKN DGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE
Sbjct: 61  KKSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 120

Query: 121 FCVLGEKLVMGFEVKQRRVEDEDEDEDE--LREAFGVFDKDKDGVISVEELGLVLSCLGM 180
           FCVLGEKLVMGFE KQRRVEDEDEDEDE  LREAFGVFDKDKDGVISVEELGLVLSCLGM
Sbjct: 121 FCVLGEKLVMGFEEKQRRVEDEDEDEDEDELREAFGVFDKDKDGVISVEELGLVLSCLGM 180

Query: 181 NEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN 220
           NEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN
Sbjct: 181 NEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN 222

[Close]

BLAST of Cp4.1LG11g10410.1 vs. NCBI nr
Match: KAG6599380.1 (Calmodulin-like protein 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 415 bits (1067), Expect = 7.27e-146
Identity = 217/222 (97.75%), Postives = 218/222 (98.20%), Query Frame = 0


Query: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEK 60
           MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEK
Sbjct: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEK 60

Query: 61  KKSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 120
           KKSEMKKVFGTFDKN DGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE
Sbjct: 61  KKSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 120

Query: 121 FCVLGEKLVMGFEVKQRRVEDEDEDEDE--LREAFGVFDKDKDGVISVEELGLVLSCLGM 180
           FCVLGEKLVMGFE KQRRVEDEDEDEDE  LREAFGVFDKDKDG+ISVEELGLVLSCLGM
Sbjct: 121 FCVLGEKLVMGFEEKQRRVEDEDEDEDEDELREAFGVFDKDKDGMISVEELGLVLSCLGM 180

Query: 181 NEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN 220
           NEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN
Sbjct: 181 NEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN 222

[Close]

BLAST of Cp4.1LG11g10410.1 vs. NCBI nr
Match: XP_022999398.1 (calmodulin-like protein 7 [Cucurbita maxima])

HSP 1 Score: 410 bits (1055), Expect = 4.25e-144
Identity = 214/220 (97.27%), Postives = 216/220 (98.18%), Query Frame = 0


Query: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEK 60
           MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPP SPAPEK
Sbjct: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPPSPAPEK 60

Query: 61  KKSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 120
           KKSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE
Sbjct: 61  KKSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 120

Query: 121 FCVLGEKLVMGFEVKQRRVEDEDEDEDELREAFGVFDKDKDGVISVEELGLVLSCLGMNE 180
           FCVLGEKLV+GFE KQRR+EDEDEDE  LREAFGVFDKDKDGVISVEELGLVLSCLGMNE
Sbjct: 121 FCVLGEKLVVGFEEKQRRIEDEDEDE--LREAFGVFDKDKDGVISVEELGLVLSCLGMNE 180

Query: 181 GKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN 220
           GKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN
Sbjct: 181 GKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN 218

[Close]

BLAST of Cp4.1LG11g10410.1 vs. NCBI nr
Match: XP_038889113.1 (calmodulin-like protein 3 [Benincasa hispida])

HSP 1 Score: 333 bits (854), Expect = 2.14e-113
Identity = 173/219 (79.00%), Postives = 191/219 (87.21%), Query Frame = 0


Query: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAV-PPLSPAPE 60
           ME +MN L L VL VAGFVNFL+YFPTKRF+AWFQSIKPSPQ  HFK  ++ PP  P P 
Sbjct: 1   MEPIMNFLLLVVLVVAGFVNFLLYFPTKRFSAWFQSIKPSPQISHFKSLSLQPPPPPPPS 60

Query: 61  KKKSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFE 120
               E+KKVFGTFDKNNDGFITKKELMESLKNM M+ITE++AEEM+KGVDENGDGLIDFE
Sbjct: 61  PPSPELKKVFGTFDKNNDGFITKKELMESLKNMRMLITEKDAEEMLKGVDENGDGLIDFE 120

Query: 121 EFCVLGEKLVMGFEVKQRRVEDEDEDEDELREAFGVFDKDKDGVISVEELGLVLSCLGMN 180
           EFCVLGEKLVMGF  K++   +++EDEDELREAFGVFDKDKDG+ISVEEL LVL  LGMN
Sbjct: 121 EFCVLGEKLVMGFGEKKKICVEDEEDEDELREAFGVFDKDKDGMISVEELSLVLCSLGMN 180

Query: 181 EGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLV 218
           EGKK+ENCKEMIKKVDLDGDGMVNFDEFKRMMRSG +LV
Sbjct: 181 EGKKVENCKEMIKKVDLDGDGMVNFDEFKRMMRSGVSLV 219

[Close]

BLAST of Cp4.1LG11g10410.1 vs. ExPASy TrEMBL
Match: A0A6J1G4Z1 (calmodulin-like protein 7 OS=Cucurbita moschata OX=3662 GN=LOC111450782 PE=4 SV=1)

HSP 1 Score: 416 bits (1070), Expect = 1.23e-146
Identity = 218/222 (98.20%), Postives = 218/222 (98.20%), Query Frame = 0


Query: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEK 60
           MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEK
Sbjct: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEK 60

Query: 61  KKSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 120
           KKSEMKKVFGTFDKN DGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE
Sbjct: 61  KKSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 120

Query: 121 FCVLGEKLVMGFEVKQRRVEDEDEDEDE--LREAFGVFDKDKDGVISVEELGLVLSCLGM 180
           FCVLGEKLVMGFE KQRRVEDEDEDEDE  LREAFGVFDKDKDGVISVEELGLVLSCLGM
Sbjct: 121 FCVLGEKLVMGFEEKQRRVEDEDEDEDEDELREAFGVFDKDKDGVISVEELGLVLSCLGM 180

Query: 181 NEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN 220
           NEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN
Sbjct: 181 NEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN 222

[Close]

BLAST of Cp4.1LG11g10410.1 vs. ExPASy TrEMBL
Match: A0A6J1KJL5 (calmodulin-like protein 7 OS=Cucurbita maxima OX=3661 GN=LOC111493780 PE=4 SV=1)

HSP 1 Score: 410 bits (1055), Expect = 2.06e-144
Identity = 214/220 (97.27%), Postives = 216/220 (98.18%), Query Frame = 0


Query: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEK 60
           MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPP SPAPEK
Sbjct: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPPSPAPEK 60

Query: 61  KKSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 120
           KKSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE
Sbjct: 61  KKSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 120

Query: 121 FCVLGEKLVMGFEVKQRRVEDEDEDEDELREAFGVFDKDKDGVISVEELGLVLSCLGMNE 180
           FCVLGEKLV+GFE KQRR+EDEDEDE  LREAFGVFDKDKDGVISVEELGLVLSCLGMNE
Sbjct: 121 FCVLGEKLVVGFEEKQRRIEDEDEDE--LREAFGVFDKDKDGVISVEELGLVLSCLGMNE 180

Query: 181 GKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN 220
           GKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN
Sbjct: 181 GKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN 218

[Close]

BLAST of Cp4.1LG11g10410.1 vs. ExPASy TrEMBL
Match: A0A0A0LL19 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G007990 PE=4 SV=1)

HSP 1 Score: 306 bits (785), Expect = 3.80e-103
Identity = 165/227 (72.69%), Postives = 189/227 (83.26%), Query Frame = 0


Query: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAV---PPLSPA 60
           ME + N L L+VLFVAGF+NFL+YFP+KRF+AWFQSIKPS Q  HFK T +   PP SP+
Sbjct: 1   MEPIFNFLLLSVLFVAGFINFLLYFPSKRFSAWFQSIKPSSQITHFKSTPLQPPPPPSPS 60

Query: 61  PEKKKS-----EMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENG 120
           P          EMKKVFGTFDKN+DGFITKKELMESLK+M MMITE++AEEM+KGVDENG
Sbjct: 61  PSPSPPPPSAMEMKKVFGTFDKNDDGFITKKELMESLKSMRMMITEKDAEEMLKGVDENG 120

Query: 121 DGLIDFEEFCVLGEKLVMGFEV-KQRRVEDEDEDEDELREAFGVFDKDKDGVISVEELGL 180
           DGLIDFEEFCVLG KL+MGFE  K+  VEDE   EDEL++AFGVFDKD DG+ISVEEL L
Sbjct: 121 DGLIDFEEFCVLGGKLMMGFEENKKTSVEDE---EDELKDAFGVFDKDSDGLISVEELSL 180

Query: 181 VLSCLGMNEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLV 218
           VL  LGMNEGK +ENCKEMI+KVDLDGDGMVNFDEFK+MMR+G T++
Sbjct: 181 VLCSLGMNEGKIVENCKEMIRKVDLDGDGMVNFDEFKKMMRNGVTIL 224

[Close]

BLAST of Cp4.1LG11g10410.1 vs. ExPASy TrEMBL
Match: A0A1S3C5X4 (calmodulin-like protein 7 OS=Cucumis melo OX=3656 GN=LOC103496824 PE=4 SV=1)

HSP 1 Score: 301 bits (772), Expect = 3.37e-101
Identity = 162/225 (72.00%), Postives = 185/225 (82.22%), Query Frame = 0


Query: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAV------PPL 60
           ME + N L L+VLFVAGFVNFL+YFPTKRFTAWFQSIKPS Q  HFK T +      PP 
Sbjct: 1   MEPIFNFLLLSVLFVAGFVNFLLYFPTKRFTAWFQSIKPSSQIPHFKSTPLQPPPPPPPP 60

Query: 61  SPAPEKKKSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDG 120
            P P     E+KKVFGTFDKN+DGFITKKELMESLK+M MMITE++AEEM+K VDENGDG
Sbjct: 61  PPPPPPSAMELKKVFGTFDKNDDGFITKKELMESLKSMRMMITEKDAEEMLKEVDENGDG 120

Query: 121 LIDFEEFCVLGEKLVMGFEV-KQRRVEDEDEDEDELREAFGVFDKDKDGVISVEELGLVL 180
           LIDFEEFCVLGEKL+MGFE  K+  V D   DE+ L++AFGVFDKD DG+ISVEEL LVL
Sbjct: 121 LIDFEEFCVLGEKLLMGFEENKKTSVGD---DEEGLKDAFGVFDKDSDGLISVEELSLVL 180

Query: 181 SCLGMNEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLV 218
             LGMNEGK +ENCKEMI+KVDLDGDGMVNFDEFK+MMR+G +++
Sbjct: 181 CSLGMNEGKIVENCKEMIRKVDLDGDGMVNFDEFKKMMRNGVSIL 222

[Close]

BLAST of Cp4.1LG11g10410.1 vs. ExPASy TrEMBL
Match: A0A6J1CMI5 (calmodulin-like protein 7 OS=Momordica charantia OX=3673 GN=LOC111012994 PE=4 SV=1)

HSP 1 Score: 265 bits (677), Expect = 4.46e-86
Identity = 145/215 (67.44%), Postives = 175/215 (81.40%), Query Frame = 0


Query: 2   ESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEKK 61
           +S+MN+L LAVLFVAG VNFL+YFPTKRF+AW   +K SP     KP AV   + AP + 
Sbjct: 67  QSMMNILLLAVLFVAGLVNFLVYFPTKRFSAW---LKQSPT----KPAAVA-AAVAPPQP 126

Query: 62  KSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEF 121
            +E++K+F TFDKNNDG+IT+KEL ESL+N    I EREAEE+ +GVDENGDGLIDFEEF
Sbjct: 127 PTELRKIFRTFDKNNDGYITRKELTESLRNW---IAEREAEELFEGVDENGDGLIDFEEF 186

Query: 122 CVLGEKLVMGFEVKQRRVEDEDEDEDELREAFGVFDKDKDGVISVEELGLVLSCLGMNEG 181
           CVL EKL      ++ + E+E++D++ELREAFGVFDKDKDGVISVEELGLVLSCLG+ EG
Sbjct: 187 CVLAEKL------RKEKEEEEEDDDEELREAFGVFDKDKDGVISVEELGLVLSCLGLKEG 246

Query: 182 KKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTT 216
           K  ENC+EMI+KVDLDGDGMVNF+EFKRMMR+G T
Sbjct: 247 KMAENCREMIRKVDLDGDGMVNFEEFKRMMRNGGT 264

[Close]

BLAST of Cp4.1LG11g10410.1 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 146.7 bits (369), Expect = 2.1e-35
Identity = 72/152 (47.37%), Postives = 112/152 (73.68%), Query Frame = 0


Query: 63  SEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEFC 122
           +E+K+VF  FDKN DG IT KEL E+L+++ + I ++E  +M++ +D NGDG +D +EF 
Sbjct: 4   TELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEF- 63

Query: 123 VLGEKLVMGFEVKQRRVEDEDEDEDELREAFGVFDKDKDGVISVEELGLVLSCLGMNEGK 182
                     E+ +  +++EDE+E++++EAF VFD++ DG I+V+EL  VLS LG+ +GK
Sbjct: 64  ---------GELYKTIMDEEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGK 123

Query: 183 KLENCKEMIKKVDLDGDGMVNFDEFKRMMRSG 215
            L++CK+MIKKVD+DGDG VN+ EF++MM+ G
Sbjct: 124 TLDDCKKMIKKVDVDGDGRVNYKEFRQMMKGG 145

[Close]

BLAST of Cp4.1LG11g10410.1 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 145.2 bits (365), Expect = 6.2e-35
Identity = 74/153 (48.37%), Postives = 111/153 (72.55%), Query Frame = 0


Query: 62  KSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEF 121
           ++E+ ++F  FD+N DG ITK+EL +SL+N+ + I +++  +M++ +D NGDG +D EEF
Sbjct: 3   QAELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEF 62

Query: 122 CVLGEKLVMGFEVKQRRVEDEDEDEDELREAFGVFDKDKDGVISVEELGLVLSCLGMNEG 181
             L + +          +E+ DE+ED +REAF VFD+++DG I+VEEL  VL+ LG+ +G
Sbjct: 63  GGLYQTI----------MEERDEEED-MREAFNVFDQNRDGFITVEELRSVLASLGLKQG 122

Query: 182 KKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSG 215
           + LE+CK MI KVD+DGDGMVNF EFK+MM+ G
Sbjct: 123 RTLEDCKRMISKVDVDGDGMVNFKEFKQMMKGG 144

[Close]

BLAST of Cp4.1LG11g10410.1 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 141.7 bits (356), Expect = 6.8e-34
Identity = 73/153 (47.71%), Postives = 105/153 (68.63%), Query Frame = 0


Query: 62  KSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEF 121
           + E+ +VF  FDKN DG I K EL +  K++ +M+ E E  EM+  +D NGDG +D +EF
Sbjct: 3   RGELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEF 62

Query: 122 CVLGEKLVMGFEVKQRRVEDEDEDEDELREAFGVFDKDKDGVISVEELGLVLSCLGMNEG 181
             L +++V           +E E+E+++REAF VFD++ DG I+ EEL  VL+ +G+ +G
Sbjct: 63  GSLYQEMV-----------EEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQG 122

Query: 182 KKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSG 215
           + LE+CK+MI KVD+DGDGMVNF EFK+MMR G
Sbjct: 123 RTLEDCKKMISKVDVDGDGMVNFKEFKQMMRGG 144

[Close]

BLAST of Cp4.1LG11g10410.1 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 140.6 bits (353), Expect = 1.5e-33
Identity = 79/190 (41.58%), Postives = 122/190 (64.21%), Query Frame = 0


Query: 32  AWF-----QSIKPSPQSVHFKPTAVPPLSPAPEKK--KSEMKKVFGTFDKNNDGFITKKE 91
           +WF     ++  PSP ++   P++    S AP K+   SE+K+VF  FDKN DG ITK+E
Sbjct: 30  SWFDKTLHKNSPPSPSTMLPSPSS----SSAPTKRIDPSELKRVFQMFDKNGDGRITKEE 89

Query: 92  LMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEFCVLGEKLVMGFEVKQRRVEDEDE 151
           L +SL+N+ + I +++  +M+  +D NGDG +D +EF  L   +V           D + 
Sbjct: 90  LNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDIDEFESLYSSIV------DEHHNDGET 149

Query: 152 DEDELREAFGVFDKDKDGVISVEELGLVLSCLGMNEGKKLENCKEMIKKVDLDGDGMVNF 211
           +E+++++AF VFD+D DG I+VEEL  V++ LG+ +GK L+ CK+MI +VD DGDG VN+
Sbjct: 150 EEEDMKDAFNVFDQDGDGFITVEELKSVMASLGLKQGKTLDGCKKMIMQVDADGDGRVNY 209

Query: 212 DEFKRMMRSG 215
            EF +MM+ G
Sbjct: 210 KEFLQMMKGG 209

[Close]

BLAST of Cp4.1LG11g10410.1 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 139.8 bits (351), Expect = 2.6e-33
Identity = 75/153 (49.02%), Postives = 106/153 (69.28%), Query Frame = 0


Query: 63  SEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEFC 122
           +E+ +VF  FDK+ DG IT KEL ES KN+ ++I E E  ++++ +D NGDG +D EEF 
Sbjct: 4   TELNRVFQMFDKDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIEEF- 63

Query: 123 VLGEKLVMGFEVKQRRVEDEDE-DEDELREAFGVFDKDKDGVISVEELGLVLSCLGMNEG 182
                   G   K   VEDEDE  E++++EAF VFD++ DG I+V+EL  VLS LG+ +G
Sbjct: 64  --------GELYKTIMVEDEDEVGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQG 123

Query: 183 KKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSG 215
           K LE C++MI +VD+DGDG VN+ EF++MM+ G
Sbjct: 124 KTLEECRKMIMQVDVDGDGRVNYMEFRQMMKKG 147

The following BLAST results are available for this feature:

BLAST of Cp4.1LG11g10410.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LNE7	3.0e-34	47.37	Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1	[more]
Q9SRR7	8.7e-34	48.37	Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1	[more]
Q9SU00	9.6e-33	47.71	Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1	[more]
O22845	2.1e-32	41.58	Calmodulin-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=CML5 PE=2 SV=2	[more]
Q9ZR02	3.7e-32	49.02	Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1	[more]

BLAST of Cp4.1LG11g10410.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023545557.1	3.64e-150	100.00	calmodulin-like protein 7 [Cucurbita pepo subsp. pepo]	[more]
XP_022946838.1	2.54e-146	98.20	calmodulin-like protein 7 [Cucurbita moschata]	[more]
KAG6599380.1	7.27e-146	97.75	Calmodulin-like protein 4, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022999398.1	4.25e-144	97.27	calmodulin-like protein 7 [Cucurbita maxima]	[more]
XP_038889113.1	2.14e-113	79.00	calmodulin-like protein 3 [Benincasa hispida]	[more]

BLAST of Cp4.1LG11g10410.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G4Z1	1.23e-146	98.20	calmodulin-like protein 7 OS=Cucurbita moschata OX=3662 GN=LOC111450782 PE=4 SV=...	[more]
A0A6J1KJL5	2.06e-144	97.27	calmodulin-like protein 7 OS=Cucurbita maxima OX=3661 GN=LOC111493780 PE=4 SV=1	[more]
A0A0A0LL19	3.80e-103	72.69	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G007990 PE=4 SV=1	[more]
A0A1S3C5X4	3.37e-101	72.00	calmodulin-like protein 7 OS=Cucumis melo OX=3656 GN=LOC103496824 PE=4 SV=1	[more]
A0A6J1CMI5	4.46e-86	67.44	calmodulin-like protein 7 OS=Momordica charantia OX=3673 GN=LOC111012994 PE=4 SV...	[more]

BLAST of Cp4.1LG11g10410.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G05990.1	2.1e-35	47.37	EF hand calcium-binding protein family	[more]
AT3G07490.1	6.2e-35	48.37	ARF-GAP domain 11	[more]
AT4G12860.1	6.8e-34	47.71	EF hand calcium-binding protein family	[more]
AT2G43290.1	1.5e-33	41.58	Calcium-binding EF-hand family protein	[more]
AT4G03290.1	2.6e-33	49.02	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 64..92 e-value: 1.8E-4 score: 30.9 coord: 100..128 e-value: 0.0058 score: 25.9 coord: 148..176 e-value: 1.8E-5 score: 34.2 coord: 186..214 e-value: 5.2E-6 score: 36.0
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 147..212 e-value: 4.2E-12 score: 46.3 coord: 63..122 e-value: 6.6E-12 score: 45.7
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 144..179 score: 16.075548
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 96..131 score: 11.723866
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 182..217 score: 14.401823
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 60..95 score: 13.341799
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 64..122 e-value: 1.14521E-15 score: 66.8025
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 149..212 e-value: 9.89685E-18 score: 72.1953
None	No IPR available	GENE3D	1.10.238.10		coord: 29..124 e-value: 3.2E-20 score: 74.2
None	No IPR available	GENE3D	1.10.238.10		coord: 129..218 e-value: 3.1E-22 score: 80.6
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 5..218
None	No IPR available	PANTHER	PTHR10891:SF796	CALCIUM-BINDING PROTEIN CML30-RELATED	coord: 5..218
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 109..121
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 157..169
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 195..207
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 73..85
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 59..214

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cp4.1LG11g10410	Cp4.1LG11g10410	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG11g10410.1:exon:001	Cp4.1LG11g10410.1:exon:001	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG11g10410.1:cds:001	Cp4.1LG11g10410.1:cds:001	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG11g10410.1	Cp4.1LG11g10410.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005509	calcium ion binding