Cp4.1LG10g10610.1 (mRNA) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG10g10610.1
Type	mRNA
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Cysteine proteinase inhibitor
Location	Cp4.1LG10: 7201234 .. 7205130 (-)
Sequence length	792
RNA-Seq Expression	Cp4.1LG10g10610.1
Synteny	Cp4.1LG10g10610.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTATTCGATTTGAGGTATGGAATACATACATTTCCTTAGCCGATAAGTTTCTTAGCATCGAAGTCACTCCATCATTTCAACTCAATTTCTTCCTCCAATATCAACTTCGCTCTTACAAACTCCTGTGTTCCAATCCCAGAAAGCGACCATGAAAGTGAATCACATACCTGCGCTGTTGCTGCTGCTTCTTCTTCCCGTGTTTGATTCGGGGTATTGCGTTCAAGACCCATTTATCAAGATGAAACTGGGCGGTGTTCGCGATTACATCGGCGCTCAGAACAGCCTTGAAATTGACGCTCTCGCTCGTTTCGCAGTCCAAGAGCACAACAAGAAACAGGTTTTTTCCTTTGTTTTCTTTGGTTTTTCAGCTCTTTATTCATTAATTTCTGGATTCGTTTCACTGCCTGATTTTCTTTCTTATTTTTGCTCACAATCTACTCACTTGCTTATTTAATTCGTTAATTAGTGGCTATTTGGTACCCATGTTTTGGTTGTTCATGCGTCGTTTTGATTTGTTGATCACCACTAGATGCGGTAGTTTAAATCGAATTCTTGACAGGCTTTTATGTCTCTTTGGTGCAGAATGGTCTTCTGGAATTTGAGCGGGTGTTGAAAGCTAAAGAACAGGTAGTTGCCGGTAAGTTGTATCATCTTACATTGGAAGCCAGTGATGGTGGTATGAAAAAGGTTTATGAAGCCAAAGTGTGGGTGAAGCCATGGATGAACTTCAAACAGTTGCAGGAATTCAAACCATGCCCATGATGGATCTTCAGGTGCCGTTCTGCTCCTCGTGTTCAAACTGGTATGGTAGGAATCATTCTGTTCTATTTTAATTGTATGTTTTGTATATCTGCATGATAGTACTAGACTATTAGTCCATTTATGGTAGGAACCTAAGATAGCGTGTCTATGTCCGACACACATTAGACACTCCGACACTCCGGCACACCCTTTTTGGACTTGTTGTAACGGCCCAAGCCTACCGCTAGCAGATATTTTCCTTTTTGGACTTTCTTTTTCGGGCTTCCCCTCAAGGTTAATAAAATGCGTCTATTAGGGAAAGGTTTCCACACTCTTATAAAGGGTGTATCATTCTCCTCCCCAACCGATGTGGGATTTCACAATCCTCCCCTTCAGGGCCCATCGCTGGCACTCGTTCCTTTCTCCAATCGATGTGGGATCCCTACCAAAACCACCCCCTTTCGGGGCCCAACGTCCTTACTGTCTACCCCCTCTGGGGAACAGCCTCCTCGCTGGCACATCGCCCGGTGTTTGGCTCTGATACCATTTGTAACGGCCCAGGCCTATTGCTAACATATATTGTCTTCTTTGGGATTTCCCTTTCGGGCTTCCCCTCAAGGTTTTTAAAACGCGTTTGCTAAGGAAAGGTCTTCACACCCTTATAAAGGGTGTTTTGTTCTTCTCCCCAACCGATATGGGATCTCACACTTGTGATGGATACTTGTTAGTACAATAAATATATGTTATACCAAATCAATATAGATCCAACATTTGTTAGACACGTACTGATCACTTGCAAGTATATTAGTATAATAAAATGATAATAGAAAAATACATCACACATTGATTTAGATATAAATGCATAAACTTGTTTGTTCTGAATTTTCTTATGCTATTGAAATGATATATATTTTAATAAATGTGTTCTTGTTGTGTACGTGTCCTAAATTTTTTAAAACATGTTGTGTCACTATGGCTGTGTCGTGTTTTGTACTTTGTATTTGTATTCGTGCTTCTTAGCTTATAATTTGGTATGTGTACATGTGTAAATTTCAATAATTTGAGTTTTGGCTTTTGAATGCTATATGGATGGAAACTATTCTGAAGAGTTGAAGAAAGATAAAGGGTGTGAGACTAGTTAATCCTCTCTTCTTTTTCTTGTTTGTTATAATTGCTGGTCTGCTTGGTTGGTAGGATGATTGCAATGAAAATGGCTCTCACACTCTTATCAAAATAACGAACTTTGTTATTAACGATACCAAGATTAATAGTGAGTCCAAGTCAGTCGCAAACTGAATTGAATTAGAAATCTTTGTATCCTTAGTGTTGAATGGTGAGTCCAACTTAGTCACACCACCTCGTGTCCGCCCCCTTTTTGGGCTCAGTCTCCTTGCTGGCACATTTTCTGGAGTGTTTGGCTCTGATATCATTTGTAACGGCCTAAGTCCATCACTAGTAGATATTGTCCTCTTTGCTTTCCCTTTCGGGCTTCCCCTTAATGTTTTTAAAATGCGTCTGCTAGGGAGAGATTTCTACACCTTTATAAAGAATGCTTCGTTCTCCTCCCCAACCGATGTGGGATCACACATTAGTGTTTGAGTTTAATTAGGTCAAAGGAAACAAGAATTTGGTGGGTTGTGTGGTCTCTCCATTTTTGTTATGTTATTTTGGTCTTCCATGGTCATCGGGAAGCATGAGAGATTTTAAAAAAAATTCTCTTTGGATCATCTTAATGAATTTGATTAGTAAAACACTCTTCCATTTGGCTAGTAGTTGTCTGCTAGTTTTCCCTGGAATCTAAGACTCTTTGGTATAACATTATTGTTAGTTTGCATATCTTCTGGTTTTGAGGTCACATCCATTTTTCTTTTGAGAATGGAGCTCGTGTTAACTTCTGGGGAGGATCGATTGATTCTGGTCGTTAACTCCGTATATACCATCTAGAAGTTTATTATGTTCTTCTTCGTCTGGTTGTGTATTATCTATTAGGAGCAGATTAAATACTATACGATTGTGTTTTGTGGGTTTTTTTTTTTTTCTCAACATGGATTGGATAAAGCTATAGCTCATCTTAAAGATTTTCTTGAGCAAGTAGTTGCAAGGATACCTCCTCGTGGGAACTGCCACTTTGACAAACGACATGGAATATTTTAGAGGGGCAAAAATAAACATAGTATGACGTAGAATCTTATGCTGAGTTGTCTCAGTTTTTATTAGTATATCATGTCAACTTGATCCCAGATCAGTTGGGGAGTGGAATGAATGAAACACTCTTTATAAGGGTGTGAAAACCTCTCCCTAGCAGACGCATTATAAAATCGTGAAACTGACGGCGATACGTAACGAACTAAAGAGGGCAATATCTCCTAGTAGTGGACTTGGGCCGTTACAAATGGTAGCAGAGCTAGACACTGGGGATGTGCCAGCAAAGACGTTGGGCCCCGAAGGGGGGTGAATTTTATGGGGGGTCCCCCATCGATTGGAGAAAGGAATGAGTGCCAACGAGGATGCTGGGCCTCGGAGAGGGGTGGATTGTGATATCCCACATTAGTTTGGGAGGACAACGAAACACCCTTTATAAGGGTGTGGAAACCTCTCCCTAGCTTTGAGGGGAAGCCTGGAAGGGAAAGGGAAAGTTACACACGACAATATCGGCTAGTAGTGGGCTTGGACCGTTACACTTTCATTCTTACCTTAGTTATTGTGTTACTGTTTAGCTAGAAATTTATCTTACAATAAATTGATATATTCACCTAGATCTTCAAATAAGGACAATATGCATCAAAATAAGAAGTACAGTAACCAAAATTCATGTGTTATGTTTTCGTTTCGTGCATTGTTAAAATGGAGTCGAGAGTCTTGTTGTGTCGTAAGATCGATTTCCGCGAATGTTGATCACCAAATGTTTGTTGTTCTTGGATGGCAGGTAAGTGAAGAGTATACATAAACATACCCTATAGCCCATGTGGGTTGCTGCTGAAGGTAAACAACAGAATAGATCTGAAACATTATAGGAACTCTGTTATATGATATGTTTATAATAATTGTGTAATATATGTATATGGAACTGGTAATCTAAGTTTGTAATCTAAGTTTGTAGAAACAATAAATAAGAGATGCATATACTTTCATGTAGAGATCCCTTCAAAGTTGGT

mRNA sequence

ATTATTCGATTTGAGGTATGGAATACATACATTTCCTTAGCCGATAAGTTTCTTAGCATCGAAGTCACTCCATCATTTCAACTCAATTTCTTCCTCCAATATCAACTTCGCTCTTACAAACTCCTGTGTTCCAATCCCAGAAAGCGACCATGAAAGTGAATCACATACCTGCGCTGTTGCTGCTGCTTCTTCTTCCCGTGTTTGATTCGGGGTATTGCGTTCAAGACCCATTTATCAAGATGAAACTGGGCGGTGTTCGCGATTACATCGGCGCTCAGAACAGCCTTGAAATTGACGCTCTCGCTCGTTTCGCAGTCCAAGAGCACAACAAGAAACAGAATGGTCTTCTGGAATTTGAGCGGGTGTTGAAAGCTAAAGAACAGGTAGTTGCCGGTAAGTTGTATCATCTTACATTGGAAGCCAGTGATGGTGGTATGAAAAAGGTTTATGAAGCCAAAGTGTGGGTGAAGCCATGGATGAACTTCAAACAGTTGCAGGAATTCAAACCATGCCCATGATGGATCTTCAGGTGCCGTTCTGCTCCTCGTGTTCAAACTGGTATGGTAAGTGAAGAGTATACATAAACATACCCTATAGCCCATGTGGGTTGCTGCTGAAGGTAAACAACAGAATAGATCTGAAACATTATAGGAACTCTGTTATATGATATGTTTATAATAATTGTGTAATATATGTATATGGAACTGGTAATCTAAGTTTGTAATCTAAGTTTGTAGAAACAATAAATAAGAGATGCATATACTTTCATGTAGAGATCCCTTCAAAGTTGGT

Coding sequence (CDS)

ATGAAAGTGAATCACATACCTGCGCTGTTGCTGCTGCTTCTTCTTCCCGTGTTTGATTCGGGGTATTGCGTTCAAGACCCATTTATCAAGATGAAACTGGGCGGTGTTCGCGATTACATCGGCGCTCAGAACAGCCTTGAAATTGACGCTCTCGCTCGTTTCGCAGTCCAAGAGCACAACAAGAAACAGAATGGTCTTCTGGAATTTGAGCGGGTGTTGAAAGCTAAAGAACAGGTAGTTGCCGGTAAGTTGTATCATCTTACATTGGAAGCCAGTGATGGTGGTATGAAAAAGGTTTATGAAGCCAAAGTGTGGGTGAAGCCATGGATGAACTTCAAACAGTTGCAGGAATTCAAACCATGCCCATGA

Protein sequence

MKVNHIPALLLLLLLPVFDSGYCVQDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHNKKQNGLLEFERVLKAKEQVVAGKLYHLTLEASDGGMKKVYEAKVWVKPWMNFKQLQEFKPCP

Homology

[Close]

BLAST of Cp4.1LG10g10610.1 vs. ExPASy Swiss-Prot
Match: Q06445 (Cysteine proteinase inhibitor OS=Vigna unguiculata OX=3917 PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 5.9e-32
Identity = 66/87 (75.86%), Postives = 75/87 (86.21%), Query Frame = 0


Query: 33  LGGVRDYIGAQNSLEIDALARFAVQEHNKKQNGLLEFERVLKAKEQVVAGKLYHLTLEAS 92
           LGG RD  G QNSLEID+LARFAV+EHNKKQN LLEF RV+ A++QVV+G LY +TLEA 
Sbjct: 4   LGGNRDVAGNQNSLEIDSLARFAVEEHNKKQNALLEFGRVVSAQQQVVSGTLYTITLEAK 63

Query: 93  DGGMKKVYEAKVWVKPWMNFKQLQEFK 120
           DGG KKVYEAKVW KPW+NFK+LQEFK
Sbjct: 64  DGGQKKVYEAKVWEKPWLNFKELQEFK 90

[Close]

BLAST of Cp4.1LG10g10610.1 vs. ExPASy Swiss-Prot
Match: Q8H0X6 (Cysteine proteinase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=CYS6 PE=1 SV=2)

HSP 1 Score: 135.2 bits (339), Expect = 5.0e-31
Identity = 68/111 (61.26%), Postives = 84/111 (75.68%), Query Frame = 0


Query: 10  LLLLLLPVFDSGYCVQDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHNKKQNGLLEF 69
           L +  L   D G+C ++  +   +GGV D    QNS E+++LARFAV EHNKK+N LLEF
Sbjct: 17  LFISSLIASDLGFCNEEMAL---VGGVGDVPANQNSGEVESLARFAVDEHNKKENALLEF 76

Query: 70  ERVLKAKEQVVAGKLYHLTLEASDGGMKKVYEAKVWVKPWMNFKQLQEFKP 121
            RV+KAKEQVVAG L+HLTLE  + G KK+YEAKVWVKPW+NFK+LQEFKP
Sbjct: 77  ARVVKAKEQVVAGTLHHLTLEILEAGQKKLYEAKVWVKPWLNFKELQEFKP 124

[Close]

BLAST of Cp4.1LG10g10610.1 vs. ExPASy Swiss-Prot
Match: Q41906 (Cysteine proteinase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=CYS3 PE=2 SV=2)

HSP 1 Score: 134.8 bits (338), Expect = 6.5e-31
Identity = 63/89 (70.79%), Postives = 76/89 (85.39%), Query Frame = 0


Query: 31  MKLGGVRDYIGAQNSLEIDALARFAVQEHNKKQNGLLEFERVLKAKEQVVAGKLYHLTLE 90
           M LGGV D  G QNS EI++LARFA+QEHNK+QN +LEF++++KA+EQVVAG +YHLTLE
Sbjct: 33  MMLGGVHDLRGNQNSGEIESLARFAIQEHNKQQNKILEFKKIVKAREQVVAGTMYHLTLE 92

Query: 91  ASDGGMKKVYEAKVWVKPWMNFKQLQEFK 120
           A +G   K +EAKVWVKPWMNFKQLQEFK
Sbjct: 93  AKEGDQTKNFEAKVWVKPWMNFKQLQEFK 121

[Close]

BLAST of Cp4.1LG10g10610.1 vs. ExPASy Swiss-Prot
Match: Q0JNR2 (Cysteine proteinase inhibitor 12 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0270100 PE=2 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 1.6e-29
Identity = 68/117 (58.12%), Postives = 84/117 (71.79%), Query Frame = 0


Query: 6   IPALLLLLLLPVFDSGYCVQDPFIKMK---LGGVRDYIGAQNSLEIDALARFAVQEHNKK 65
           +P  LLL +     + + V    + M    LGG  D   A NS+E DALARFAV EHNK+
Sbjct: 18  LPLFLLLAVAAAAAALFLVGSASLAMAGHVLGGAHDAPSAANSVETDALARFAVDEHNKR 77

Query: 66  QNGLLEFERVLKAKEQVVAGKLYHLTLEASDGGMKKVYEAKVWVKPWMNFKQLQEFK 120
           +N LLEF RV++AKEQVVAG L+HLTLEA + G KKVYEAKVWVKPW++FK+LQEF+
Sbjct: 78  ENALLEFVRVVEAKEQVVAGTLHHLTLEALEAGRKKVYEAKVWVKPWLDFKELQEFR 134

[Close]

BLAST of Cp4.1LG10g10610.1 vs. ExPASy Swiss-Prot
Match: Q10992 (Cysteine proteinase inhibitor A OS=Helianthus annuus OX=4232 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 1.4e-28
Identity = 59/76 (77.63%), Postives = 68/76 (89.47%), Query Frame = 0


Query: 45  SLEIDALARFAVQEHNKKQNGLLEFERVLKAKEQVVAGKLYHLTLEASDGGMKKVYEAKV 104
           SLEID LARFAV EHNKKQN LLEF +V+  KEQVVAGK+Y++TLEA++GG+KK YEAKV
Sbjct: 1   SLEIDELARFAVDEHNKKQNALLEFGKVVNTKEQVVAGKMYYITLEATNGGVKKTYEAKV 60

Query: 105 WVKPWMNFKQLQEFKP 121
           WVKPW NFK+LQEFKP
Sbjct: 61  WVKPWENFKELQEFKP 76

[Close]

BLAST of Cp4.1LG10g10610.1 vs. NCBI nr
Match: XP_023543498.1 (cysteine proteinase inhibitor A-like [Cucurbita pepo subsp. pepo] >XP_023543500.1 cysteine proteinase inhibitor A-like [Cucurbita pepo subsp. pepo] >XP_023543501.1 cysteine proteinase inhibitor A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 251 bits (642), Expect = 2.62e-84
Identity = 122/122 (100.00%), Postives = 122/122 (100.00%), Query Frame = 0


Query: 1   MKVNHIPALLLLLLLPVFDSGYCVQDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60
           MKVNHIPALLLLLLLPVFDSGYCVQDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN
Sbjct: 1   MKVNHIPALLLLLLLPVFDSGYCVQDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60

Query: 61  KKQNGLLEFERVLKAKEQVVAGKLYHLTLEASDGGMKKVYEAKVWVKPWMNFKQLQEFKP 120
           KKQNGLLEFERVLKAKEQVVAGKLYHLTLEASDGGMKKVYEAKVWVKPWMNFKQLQEFKP
Sbjct: 61  KKQNGLLEFERVLKAKEQVVAGKLYHLTLEASDGGMKKVYEAKVWVKPWMNFKQLQEFKP 120

Query: 121 CP 122
           CP
Sbjct: 121 CP 122

[Close]

BLAST of Cp4.1LG10g10610.1 vs. NCBI nr
Match: XP_022932782.1 (cysteine proteinase inhibitor A-like [Cucurbita moschata] >XP_022932783.1 cysteine proteinase inhibitor A-like [Cucurbita moschata] >XP_022932784.1 cysteine proteinase inhibitor A-like [Cucurbita moschata] >KAG7034455.1 hypothetical protein SDJN02_04184 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 240 bits (612), Expect = 9.89e-80
Identity = 115/122 (94.26%), Postives = 120/122 (98.36%), Query Frame = 0


Query: 1   MKVNHIPALLLLLLLPVFDSGYCVQDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60
           MKVNHI A+LLLLLLP+FDSGYCV+DPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN
Sbjct: 1   MKVNHIAAVLLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60

Query: 61  KKQNGLLEFERVLKAKEQVVAGKLYHLTLEASDGGMKKVYEAKVWVKPWMNFKQLQEFKP 120
           KK+NGLLEFERVLKAKEQVVAGKLYHLTLEA DGG+KKVYEAKVWVKPWMNFKQLQEFKP
Sbjct: 61  KKENGLLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFKP 120

Query: 121 CP 122
           CP
Sbjct: 121 CP 122

[Close]

BLAST of Cp4.1LG10g10610.1 vs. NCBI nr
Match: XP_022967919.1 (cysteine proteinase inhibitor A-like [Cucurbita maxima] >XP_022967920.1 cysteine proteinase inhibitor A-like [Cucurbita maxima] >XP_022967921.1 cysteine proteinase inhibitor A-like [Cucurbita maxima])

HSP 1 Score: 238 bits (606), Expect = 8.14e-79
Identity = 114/121 (94.21%), Postives = 119/121 (98.35%), Query Frame = 0


Query: 1   MKVNHIPALLLLLLLPVFDSGYCVQDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60
           MKVNHI A+LL+LLLPVFDSGYCV+DPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN
Sbjct: 1   MKVNHIAAVLLVLLLPVFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60

Query: 61  KKQNGLLEFERVLKAKEQVVAGKLYHLTLEASDGGMKKVYEAKVWVKPWMNFKQLQEFKP 120
           KK+NGLLEFERVLKAKEQVV+GKLYHLTLEA DGGMKKVYEAKVWVKPWMNFKQLQEFKP
Sbjct: 61  KKENGLLEFERVLKAKEQVVSGKLYHLTLEAIDGGMKKVYEAKVWVKPWMNFKQLQEFKP 120

[Close]

BLAST of Cp4.1LG10g10610.1 vs. NCBI nr
Match: XP_038880981.1 (cysteine proteinase inhibitor A-like [Benincasa hispida])

HSP 1 Score: 215 bits (548), Expect = 1.26e-69
Identity = 105/122 (86.07%), Postives = 111/122 (90.98%), Query Frame = 0


Query: 1   MKVNHIPALLLLLLLPVFDSGYCVQDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60
           MKVN I   LLLLLL VFD G+ V+DPFI+MKLGGVRDYIGAQNS+EID LARFAVQEHN
Sbjct: 25  MKVNRIAVALLLLLLAVFDLGFSVEDPFIRMKLGGVRDYIGAQNSVEIDYLARFAVQEHN 84

Query: 61  KKQNGLLEFERVLKAKEQVVAGKLYHLTLEASDGGMKKVYEAKVWVKPWMNFKQLQEFKP 120
           KK+NGLLEFERVLKAKEQV+AGKLYHLTLEA DGG KKVYEAKVWVKPWMNFKQLQEFK 
Sbjct: 85  KKENGLLEFERVLKAKEQVIAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFKQLQEFKL 144

Query: 121 CP 122
           CP
Sbjct: 145 CP 146

[Close]

BLAST of Cp4.1LG10g10610.1 vs. NCBI nr
Match: XP_008455746.1 (PREDICTED: cysteine proteinase inhibitor A [Cucumis melo] >XP_016901818.1 PREDICTED: cysteine proteinase inhibitor A [Cucumis melo] >XP_016901819.1 PREDICTED: cysteine proteinase inhibitor A [Cucumis melo] >KAA0025834.1 cysteine proteinase inhibitor A [Cucumis melo var. makuwa] >TYK09622.1 cysteine proteinase inhibitor A [Cucumis melo var. makuwa])

HSP 1 Score: 204 bits (519), Expect = 1.51e-65
Identity = 97/121 (80.17%), Postives = 108/121 (89.26%), Query Frame = 0


Query: 1   MKVNHIPALLLLLLLPVFDSGYCVQDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60
           MK+NHI   LL LLL  FD G+ ++DPFI+MKLGGVRDYIG+QN +EI++LARFAVQEHN
Sbjct: 1   MKLNHIAVPLLFLLLAAFDLGFSIEDPFIRMKLGGVRDYIGSQNRVEIESLARFAVQEHN 60

Query: 61  KKQNGLLEFERVLKAKEQVVAGKLYHLTLEASDGGMKKVYEAKVWVKPWMNFKQLQEFKP 120
            K+NGLLEF+RVLKAKEQVVAGKLYHLTLEA DGG KKVYEAKVWVKPWMNFKQLQEFK 
Sbjct: 61  NKENGLLEFDRVLKAKEQVVAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFKQLQEFKL 120

[Close]

BLAST of Cp4.1LG10g10610.1 vs. ExPASy TrEMBL
Match: A0A6J1F2Q3 (Cysteine proteinase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111439234 PE=3 SV=1)

HSP 1 Score: 240 bits (612), Expect = 4.79e-80
Identity = 115/122 (94.26%), Postives = 120/122 (98.36%), Query Frame = 0


Query: 1   MKVNHIPALLLLLLLPVFDSGYCVQDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60
           MKVNHI A+LLLLLLP+FDSGYCV+DPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN
Sbjct: 1   MKVNHIAAVLLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60

Query: 61  KKQNGLLEFERVLKAKEQVVAGKLYHLTLEASDGGMKKVYEAKVWVKPWMNFKQLQEFKP 120
           KK+NGLLEFERVLKAKEQVVAGKLYHLTLEA DGG+KKVYEAKVWVKPWMNFKQLQEFKP
Sbjct: 61  KKENGLLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFKP 120

Query: 121 CP 122
           CP
Sbjct: 121 CP 122

[Close]

BLAST of Cp4.1LG10g10610.1 vs. ExPASy TrEMBL
Match: A0A6J1HVR4 (Cysteine proteinase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111467290 PE=3 SV=1)

HSP 1 Score: 238 bits (606), Expect = 3.94e-79
Identity = 114/121 (94.21%), Postives = 119/121 (98.35%), Query Frame = 0


Query: 1   MKVNHIPALLLLLLLPVFDSGYCVQDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60
           MKVNHI A+LL+LLLPVFDSGYCV+DPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN
Sbjct: 1   MKVNHIAAVLLVLLLPVFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60

Query: 61  KKQNGLLEFERVLKAKEQVVAGKLYHLTLEASDGGMKKVYEAKVWVKPWMNFKQLQEFKP 120
           KK+NGLLEFERVLKAKEQVV+GKLYHLTLEA DGGMKKVYEAKVWVKPWMNFKQLQEFKP
Sbjct: 61  KKENGLLEFERVLKAKEQVVSGKLYHLTLEAIDGGMKKVYEAKVWVKPWMNFKQLQEFKP 120

[Close]

BLAST of Cp4.1LG10g10610.1 vs. ExPASy TrEMBL
Match: A0A1S4E0R5 (Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103495847 PE=3 SV=1)

HSP 1 Score: 204 bits (519), Expect = 7.31e-66
Identity = 97/121 (80.17%), Postives = 108/121 (89.26%), Query Frame = 0


Query: 1   MKVNHIPALLLLLLLPVFDSGYCVQDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60
           MK+NHI   LL LLL  FD G+ ++DPFI+MKLGGVRDYIG+QN +EI++LARFAVQEHN
Sbjct: 1   MKLNHIAVPLLFLLLAAFDLGFSIEDPFIRMKLGGVRDYIGSQNRVEIESLARFAVQEHN 60

Query: 61  KKQNGLLEFERVLKAKEQVVAGKLYHLTLEASDGGMKKVYEAKVWVKPWMNFKQLQEFKP 120
            K+NGLLEF+RVLKAKEQVVAGKLYHLTLEA DGG KKVYEAKVWVKPWMNFKQLQEFK 
Sbjct: 61  NKENGLLEFDRVLKAKEQVVAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFKQLQEFKL 120

[Close]

BLAST of Cp4.1LG10g10610.1 vs. ExPASy TrEMBL
Match: A0A5D3CEB5 (Cysteine proteinase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold447G00160 PE=3 SV=1)

HSP 1 Score: 204 bits (519), Expect = 7.31e-66
Identity = 97/121 (80.17%), Postives = 108/121 (89.26%), Query Frame = 0


Query: 1   MKVNHIPALLLLLLLPVFDSGYCVQDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60
           MK+NHI   LL LLL  FD G+ ++DPFI+MKLGGVRDYIG+QN +EI++LARFAVQEHN
Sbjct: 1   MKLNHIAVPLLFLLLAAFDLGFSIEDPFIRMKLGGVRDYIGSQNRVEIESLARFAVQEHN 60

Query: 61  KKQNGLLEFERVLKAKEQVVAGKLYHLTLEASDGGMKKVYEAKVWVKPWMNFKQLQEFKP 120
            K+NGLLEF+RVLKAKEQVVAGKLYHLTLEA DGG KKVYEAKVWVKPWMNFKQLQEFK 
Sbjct: 61  NKENGLLEFDRVLKAKEQVVAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFKQLQEFKL 120

[Close]

BLAST of Cp4.1LG10g10610.1 vs. ExPASy TrEMBL
Match: A0FK05 (Cysteine proteinase inhibitor OS=Populus tomentosa OX=118781 PE=2 SV=1)

HSP 1 Score: 167 bits (422), Expect = 7.35e-51
Identity = 79/101 (78.22%), Postives = 87/101 (86.14%), Query Frame = 0


Query: 19  DSGYCVQDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHNKKQNGLLEFERVLKAKEQ 78
           + G C QD F+KMKLGGV D  G+QNS EID+LARFAVQEHNKK+N +LEF RVLKAKEQ
Sbjct: 22  ELGLCRQDNFLKMKLGGVHDCRGSQNSAEIDSLARFAVQEHNKKENAILEFVRVLKAKEQ 81

Query: 79  VVAGKLYHLTLEASDGGMKKVYEAKVWVKPWMNFKQLQEFK 119
           VVAGKLYHLTLEA+D G  K+YE KVWVKPWMNFKQLQEFK
Sbjct: 82  VVAGKLYHLTLEATDAGNNKMYEVKVWVKPWMNFKQLQEFK 122

[Close]

BLAST of Cp4.1LG10g10610.1 vs. TAIR 10
Match: AT3G12490.2 (cystatin B )

HSP 1 Score: 135.2 bits (339), Expect = 3.6e-32
Identity = 68/111 (61.26%), Postives = 84/111 (75.68%), Query Frame = 0


Query: 10  LLLLLLPVFDSGYCVQDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHNKKQNGLLEF 69
           L +  L   D G+C ++  +   +GGV D    QNS E+++LARFAV EHNKK+N LLEF
Sbjct: 17  LFISSLIASDLGFCNEEMAL---VGGVGDVPANQNSGEVESLARFAVDEHNKKENALLEF 76

Query: 70  ERVLKAKEQVVAGKLYHLTLEASDGGMKKVYEAKVWVKPWMNFKQLQEFKP 121
            RV+KAKEQVVAG L+HLTLE  + G KK+YEAKVWVKPW+NFK+LQEFKP
Sbjct: 77  ARVVKAKEQVVAGTLHHLTLEILEAGQKKLYEAKVWVKPWLNFKELQEFKP 124

[Close]

BLAST of Cp4.1LG10g10610.1 vs. TAIR 10
Match: AT2G40880.1 (cystatin A )

HSP 1 Score: 134.8 bits (338), Expect = 4.6e-32
Identity = 63/89 (70.79%), Postives = 76/89 (85.39%), Query Frame = 0


Query: 31  MKLGGVRDYIGAQNSLEIDALARFAVQEHNKKQNGLLEFERVLKAKEQVVAGKLYHLTLE 90
           M LGGV D  G QNS EI++LARFA+QEHNK+QN +LEF++++KA+EQVVAG +YHLTLE
Sbjct: 33  MMLGGVHDLRGNQNSGEIESLARFAIQEHNKQQNKILEFKKIVKAREQVVAGTMYHLTLE 92

Query: 91  ASDGGMKKVYEAKVWVKPWMNFKQLQEFK 120
           A +G   K +EAKVWVKPWMNFKQLQEFK
Sbjct: 93  AKEGDQTKNFEAKVWVKPWMNFKQLQEFK 121

[Close]

BLAST of Cp4.1LG10g10610.1 vs. TAIR 10
Match: AT3G12490.1 (cystatin B )

HSP 1 Score: 133.3 bits (334), Expect = 1.4e-31
Identity = 63/88 (71.59%), Postives = 74/88 (84.09%), Query Frame = 0


Query: 33  LGGVRDYIGAQNSLEIDALARFAVQEHNKKQNGLLEFERVLKAKEQVVAGKLYHLTLEAS 92
           +GGV D    QNS E+++LARFAV EHNKK+N LLEF RV+KAKEQVVAG L+HLTLE  
Sbjct: 4   VGGVGDVPANQNSGEVESLARFAVDEHNKKENALLEFARVVKAKEQVVAGTLHHLTLEIL 63

Query: 93  DGGMKKVYEAKVWVKPWMNFKQLQEFKP 121
           + G KK+YEAKVWVKPW+NFK+LQEFKP
Sbjct: 64  EAGQKKLYEAKVWVKPWLNFKELQEFKP 91

[Close]

BLAST of Cp4.1LG10g10610.1 vs. TAIR 10
Match: AT5G05110.1 (Cystatin/monellin family protein )

HSP 1 Score: 125.9 bits (315), Expect = 2.2e-29
Identity = 64/95 (67.37%), Postives = 72/95 (75.79%), Query Frame = 0


Query: 26  DPFIKMKLGGVRDYIGAQN-SLEIDALARFAVQEHNKKQNGLLEFERVLKAKEQVVAGKL 85
           D  +KMKLGG  D     N   EID +A FAVQEHN+++N +LE  RVLKA EQVVAGKL
Sbjct: 38  DNVVKMKLGGFSDSKNDWNGGKEIDDIALFAVQEHNRRENAVLELARVLKATEQVVAGKL 97

Query: 86  YHLTLEASDGGMKKVYEAKVWVKPWMNFKQLQEFK 120
           Y LTLE  + G KK+YEAKVWVKPWMNFKQLQEFK
Sbjct: 98  YRLTLEVIEAGEKKIYEAKVWVKPWMNFKQLQEFK 132

[Close]

BLAST of Cp4.1LG10g10610.1 vs. TAIR 10
Match: AT5G12140.1 (cystatin-1 )

HSP 1 Score: 101.7 bits (252), Expect = 4.4e-22
Identity = 49/86 (56.98%), Postives = 63/86 (73.26%), Query Frame = 0


Query: 33  LGGVRDYIGAQNSLEIDALARFAVQEHNKKQNGLLEFERVLKAKEQVVAGKLYHLTLEAS 92
           +GGVRD     N L++++LARFAV EHNK +N  LE++R+L AK QVVAG ++HLT+E +
Sbjct: 10  VGGVRDIDANANDLQVESLARFAVDEHNKNENLTLEYKRLLGAKTQVVAGTMHHLTVEVA 69

Query: 93  DGGMKKVYEAKVWVKPWMNFKQLQEF 119
           DG   KVYEAKV  K W N KQL+ F
Sbjct: 70  DGETNKVYEAKVLEKAWENLKQLESF 95

The following BLAST results are available for this feature:

BLAST of Cp4.1LG10g10610.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q06445	5.9e-32	75.86	Cysteine proteinase inhibitor OS=Vigna unguiculata OX=3917 PE=1 SV=1	[more]
Q8H0X6	5.0e-31	61.26	Cysteine proteinase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=CYS6 PE=1 SV=...	[more]
Q41906	6.5e-31	70.79	Cysteine proteinase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=CYS3 PE=2 SV=...	[more]
Q0JNR2	1.6e-29	58.12	Cysteine proteinase inhibitor 12 OS=Oryza sativa subsp. japonica OX=39947 GN=Os0...	[more]
Q10992	1.4e-28	77.63	Cysteine proteinase inhibitor A OS=Helianthus annuus OX=4232 PE=1 SV=1	[more]

BLAST of Cp4.1LG10g10610.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023543498.1	2.62e-84	100.00	cysteine proteinase inhibitor A-like [Cucurbita pepo subsp. pepo] >XP_023543500....	[more]
XP_022932782.1	9.89e-80	94.26	cysteine proteinase inhibitor A-like [Cucurbita moschata] >XP_022932783.1 cystei...	[more]
XP_022967919.1	8.14e-79	94.21	cysteine proteinase inhibitor A-like [Cucurbita maxima] >XP_022967920.1 cysteine...	[more]
XP_038880981.1	1.26e-69	86.07	cysteine proteinase inhibitor A-like [Benincasa hispida]	[more]
XP_008455746.1	1.51e-65	80.17	PREDICTED: cysteine proteinase inhibitor A [Cucumis melo] >XP_016901818.1 PREDIC...	[more]

BLAST of Cp4.1LG10g10610.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F2Q3	4.79e-80	94.26	Cysteine proteinase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111439234 PE=3...	[more]
A0A6J1HVR4	3.94e-79	94.21	Cysteine proteinase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111467290 PE=3 S...	[more]
A0A1S4E0R5	7.31e-66	80.17	Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103495847 PE=3 SV=1	[more]
A0A5D3CEB5	7.31e-66	80.17	Cysteine proteinase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0FK05	7.35e-51	78.22	Cysteine proteinase inhibitor OS=Populus tomentosa OX=118781 PE=2 SV=1	[more]

BLAST of Cp4.1LG10g10610.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12490.2	3.6e-32	61.26	cystatin B	[more]
AT2G40880.1	4.6e-32	70.79	cystatin A	[more]
AT3G12490.1	1.4e-31	71.59	cystatin B	[more]
AT5G05110.1	2.2e-29	67.37	Cystatin/monellin family protein	[more]
AT5G12140.1	4.4e-22	56.98	cystatin-1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	SMART	SM00043	CY_4	coord: 31..121 e-value: 3.3E-27 score: 106.4
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 46..121 e-value: 4.9E-24 score: 84.5
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 44..120 e-value: 8.24351E-22 score: 80.9812
None	No IPR available	GENE3D	3.10.450.10		coord: 30..122 e-value: 2.6E-40 score: 138.9
None	No IPR available	PANTHER	PTHR11413:SF110	CYSTEINE PROTEINASE INHIBITOR 7	coord: 31..120
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 40..121
IPR027214	Cystatin	PANTHER	PTHR11413	CYSTATIN FAMILY MEMBER	coord: 31..120
IPR018073	Proteinase inhibitor I25, cystatin, conserved site	PROSITE	PS00287	CYSTATIN	coord: 77..90

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cp4.1LG10g10610	Cp4.1LG10g10610	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG10g10610.1:exon:003	Cp4.1LG10g10610.1:exon:003	exon
Cp4.1LG10g10610.1:exon:002	Cp4.1LG10g10610.1:exon:002	exon
Cp4.1LG10g10610.1:exon:001	Cp4.1LG10g10610.1:exon:001	exon

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG10g10610.1:three_prime_utr:002	Cp4.1LG10g10610.1:three_prime_utr:002	three_prime_UTR
Cp4.1LG10g10610.1:three_prime_utr:001	Cp4.1LG10g10610.1:three_prime_utr:001	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG10g10610.1:cds:002	Cp4.1LG10g10610.1:cds:002	CDS
Cp4.1LG10g10610.1:cds:001	Cp4.1LG10g10610.1:cds:001	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG10g10610.1:five_prime_utr:001	Cp4.1LG10g10610.1:five_prime_utr:001	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG10g10610.1	Cp4.1LG10g10610.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006972	hyperosmotic response
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009409	response to cold
biological_process	GO:0006979	response to oxidative stress
biological_process	GO:0009414	response to water deprivation
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity