Cp4.1LG10g03250.1 (mRNA) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG10g03250.1
TypemRNA
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionTFIIS N-terminal domain-containing protein
LocationCp4.1LG10: 2095567 .. 2098744 (-)
Sequence length1979
RNA-Seq ExpressionCp4.1LG10g03250.1
SyntenyCp4.1LG10g03250.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTCCTAATGGATTATGACGATTTTGCTTTTCCGCCACTGGGTTCTCTCTGGAATTTCTAGCGTTTTGAATAGTGAACTTCCTTCTTAGATCGGTTTATGTTTTCAGAGTCCACTACTGAAAACGAGAAGTACAAACGTTTTTCGGATTGTTTATCGGGAAGATAACCTTTCTCAATCGCCGATTCTTCTAATCGGATTCACTATAAAATGGTTCGGTTGGATTTAGATGATTTCCGGACCATATTGGATACAGCCGGTGTCGATGTTTGGACCTTCATCGATACAGCTATGGAGGTTGCTTCATTGGATTACGGCAATCAATTGAAGAATCGGAGAGACGGAATCGTTGAGAGGCTCTATGCGTTGACTTCGCCTCCTACTCGATGTCGGAATTGCGATACGGATCGCTATAACGACGGCCGTTCTAATGGCTGTGAAATCAAACAGAGCCCCGTTGAGGCCAAGGAAGCTTCCCCTTCGACGCCGCAGTTCGTTGTAGTCGAAGGAGACGACGAGGGAGTAGACCCGTATGGAGGTTTGTTTGATGATGAACAGAAGAAGGTTTTAGAAATAAAGGAGCAGCTTGAAATTCCTCACCAGGTATTCTTCTTTTGATTATGATTATGATTATCTAGAATTGTTTGATTGTTTTTTGGGGAAATTCTCTTAACTTACCCAATTGGTGATTGTTTGGCTTGTTTCAGCCTGAAGATGCCTTGGTTGAACTGCTTCAAAACTTGGCAGACATGGATATAACATTCCAAGCTCTTAAGGTGATTGTTTATGGTGTTTTTTCTTGTTCTTTGTGAATGTAATTCTTTTGAATTTCTGGGTTCTGTTGCTTCCTTCTTCTTCTTCTATGTTTCTTAATTGTTCTTGTTCTTGTTCTTGTTTTTGTATAGGAAACTGATATTGGAAGGCATGTGAACCGGTTGAGGAAGCATTCATCTAATGATGTTCGCAGATTGGTGAAACATCTTGTCAGGTTCGTATAAGCTTTACAAATGGATAAAATGGTTGATGCTTGGTAAATTTCAGAGAATGGTTGGTGAATCATCAGCTCAAACGGTGTTAATAACTTCAATTTGTTTTGGTGTTGTTCATCAGAAAATGGAAGGAGATTGTGGATGAATGGGTCAGGTTGAATCAGCCTGGGGAGCAGACAGCTACACTCATAGGTAGCAAATATATGAACACCAGTTTTAACTTCATGATTCAATTCATTCAAAATCTTAATCTTAGTCACTCTGTTGCCAAAAAATTTAGCTAAGAATGGTTTCTACTGTTATTGACAGCTGATAGTGACTCACCCCAGCAAAAGGTGCCTCAAAATGGTTATCACCAGGTGAACGGAAATCATTGATCTTATGGGTTCGGATGTTTGTCTTCTGTACGATTGTGTTTATAATGTTTCGTTGTTGTGTAGGTTCCTGATTTTGCATACTCTCCGAATCCACACAGTGAGTATTGCATTATAATAATATGCAGATTTCAGCTTCTTGTTTCACTTGCCATGATTTTCCACGGATTGATTTGGTTTCTCATTTTGTCCCCCCTTCTTTGTCTTGTTAGATGGAGGTTCTGGGTCAGATAGAAATAACGTAGAACCCGAACCGAAGGGAAAGTCCGTTCCAAGAAGAGATGCCCCACCAAAACCAGCACAACCGGCTCCAATGTCGTCATATACTCCTCAAAATGTATGGTAACCAAATTAGAGTCTTTTAACTCTATGTTTGGTTGCCAAAAATGGAGGAAGAAAATTGAACTTACGATTCTTATGCTTCCGAAAACATAACAAACTTAACTCAAACTGCATCAATTGTGTTGAAATTTGACAAAATTTGAGTGGTAGGTCTAAATTTTCTGAGGTCCTAGATGATATGCCTGGTCTTACTTACAGTCTGCCAAATTTGTCTTCAGCAAATAGCTGTTTTAGGAAGATTTTCTTATTATGTCTGTTGAATTGATGGCCATTTGCAGAGACAGAAAGAACAACAGAAGGAAGCAAATTTTGATTCCCAAAAGCTTGCATCAGCTAGAAAAAGGCTTCAAGAAAATTACAAAGAAGCTGAAAATGGTTTGGCAACAGATTTCTTGCCTTTTATTTCGTTTCTGATTCTTTTGGAACAAATCAATACTAATTGCTCTGCTTGTCTGTTTCCTTTATCGAAGCTAAAAAGCAAAGAACAATCCAAGTAATGGACATTCATGAGATCCCAAAGCCAAAAAATACCTTCTTCTCAAAGAACAAAGGCACCGGCGGTGGCTCTCAAGGCAGACATTGGTGATATTTGGCAATGCAGCAAAGCAACATCTTCAAAATTAGGTCTCGGTATCGTCGAAACTGGAATCGGTTTATCATCCTTTCTATTTGTTACCTTTTTGATCATTAAAATTTAAACTCCTCTATCAAGCTTAAAGAGAATCAAAAACTACTTTTCGCGTAAATTTTCTCCTAATAAGCAATGCTAATGCTCTGATATATTGTTTATTTAGAATCCCCAAGTCTTAAAATCTCATTGGTGTAATTGGATCAACTTCAAATATAAAACTCAAATAGGAGGAAGGCCTTGTTCTTATGTCCATTGTTGTTTTCTGGTTTGGATCATATCAACAGGGGCAGCAGGGTAGAGGGTAGAGAGGTGGCTCATTAGTCATATTTGACCTTAAAAAAAGATTATTTATTACAGCAACTTAAGGTTTGGGGGCCAACACATGTGGATGGGGTTTGGTTTGTTGGCTTGCAAGTGGTCACTCCCTTATTTTCTTGATATAATATTATGGAACATGGGGCTTGGCCTAACATAAAGAAGGGTGGTTTTCCACATACAAAAAAGCTTCTACTTTTCAAGGGTGTGGGGTAGAGGATGAGAAACTTGATGCTCATTCAAAGATCTTGAAGATATCTCTTTTTGACAAAGGATAAATCTCGCAAAAGCTGTATATTTCATTAGTGTTTGGACTTGAATCAACCTTGCTTTATGATATTCTATGTTTGAATAGGTAATTTAGGGTGTAAAACCAGTAAGGGGGACCTCTTTTTACCTTTAGAATCTTTCCACTGTGAAGTGATTTATTTACTTCAAGAACAACACCCGGTAGATGCTTTCATAGCTTAAAGCCACCCAATGTGATTGATGATAAGCTTAATACAATACTTTCGTACTTTCTTTTTA

mRNA sequence

ATTTCCTAATGGATTATGACGATTTTGCTTTTCCGCCACTGGAGTCCACTACTGAAAACGAGAAGTACAAACATGATTTCCGGACCATATTGGATACAGCCGGTGTCGATGTTTGGACCTTCATCGATACAGCTATGGAGGTTGCTTCATTGGATTACGGCAATCAATTGAAGAATCGGAGAGACGGAATCGTTGAGAGGCTCTATGCGTTGACTTCGCCTCCTACTCGATGTCGGAATTGCGATACGGATCGCTATAACGACGGCCGTTCTAATGGCTGTGAAATCAAACAGAGCCCCGTTGAGGCCAAGGAAGCTTCCCCTTCGACGCCGCAGTTCGTTGTAGTCGAAGGAGACGACGAGGGAGTAGACCCGTATGGAGGTTTGTTTGATGATGAACAGAAGAAGGTTTTAGAAATAAAGGAGCAGCTTGAAATTCCTCACCAGCCTGAAGATGCCTTGGTTGAACTGCTTCAAAACTTGGCAGACATGGATATAACATTCCAAGCTCTTAAGGAAACTGATATTGGAAGGCATGTGAACCGGTTGAGGAAGCATTCATCTAATGATGTTCGCAGATTGGTGAAACATCTTGTCAGAAAATGGAAGGAGATTGTGGATGAATGGGTCAGGTTGAATCAGCCTGGGGAGCAGACAGCTACACTCATAGCTGATAGTGACTCACCCCAGCAAAAGGTGCCTCAAAATGGTTATCACCAGGTTCCTGATTTTGCATACTCTCCGAATCCACACAATGGAGGTTCTGGGTCAGATAGAAATAACGTAGAACCCGAACCGAAGGGAAAGTCCGTTCCAAGAAGAGATGCCCCACCAAAACCAGCACAACCGGCTCCAATGTCGTCATATACTCCTCAAAATAGACAGAAAGAACAACAGAAGGAAGCAAATTTTGATTCCCAAAAGCTTGCATCAGCTAGAAAAAGGCTTCAAGAAAATTACAAAGAAGCTGAAAATGCTAAAAAGCAAAGAACAATCCAAGTAATGGACATTCATGAGATCCCAAAGCCAAAAAATACCTTCTTCTCAAAGAACAAAGGCACCGGCGGTGGCTCTCAAGGCAGACATTGGTGATATTTGGCAATGCAGCAAAGCAACATCTTCAAAATTAGGTCTCGGTATCGTCGAAACTGGAATCGGTTTATCATCCTTTCTATTTGTTACCTTTTTGATCATTAAAATTTAAACTCCTCTATCAAGCTTAAAGAGAATCAAAAACTACTTTTCGCGTAAATTTTCTCCTAATAAGCAATGCTAATGCTCTGATATATTGTTTATTTAGAATCCCCAAGTCTTAAAATCTCATTGGTGTAATTGGATCAACTTCAAATATAAAACTCAAATAGGAGGAAGGCCTTGTTCTTATGTCCATTGTTGTTTTCTGGTTTGGATCATATCAACAGGGGCAGCAGGGTAGAGGGTAGAGAGGTGGCTCATTAGTCATATTTGACCTTAAAAAAAGATTATTTATTACAGCAACTTAAGGTTTGGGGGCCAACACATGTGGATGGGGTTTGGTTTGTTGGCTTGCAAGTGGTCACTCCCTTATTTTCTTGATATAATATTATGGAACATGGGGCTTGGCCTAACATAAAGAAGGGTGGTTTTCCACATACAAAAAAGCTTCTACTTTTCAAGGGTGTGGGGTAGAGGATGAGAAACTTGATGCTCATTCAAAGATCTTGAAGATATCTCTTTTTGACAAAGGATAAATCTCGCAAAAGCTGTATATTTCATTAGTGTTTGGACTTGAATCAACCTTGCTTTATGATATTCTATGTTTGAATAGGTAATTTAGGGTGTAAAACCAGTAAGGGGGACCTCTTTTTACCTTTAGAATCTTTCCACTGTGAAGTGATTTATTTACTTCAAGAACAACACCCGGTAGATGCTTTCATAGCTTAAAGCCACCCAATGTGATTGATGATAAGCTTAATACAATACTTTCGTACTTTCTTTTTA

Coding sequence (CDS)

ATGGATTATGACGATTTTGCTTTTCCGCCACTGGAGTCCACTACTGAAAACGAGAAGTACAAACATGATTTCCGGACCATATTGGATACAGCCGGTGTCGATGTTTGGACCTTCATCGATACAGCTATGGAGGTTGCTTCATTGGATTACGGCAATCAATTGAAGAATCGGAGAGACGGAATCGTTGAGAGGCTCTATGCGTTGACTTCGCCTCCTACTCGATGTCGGAATTGCGATACGGATCGCTATAACGACGGCCGTTCTAATGGCTGTGAAATCAAACAGAGCCCCGTTGAGGCCAAGGAAGCTTCCCCTTCGACGCCGCAGTTCGTTGTAGTCGAAGGAGACGACGAGGGAGTAGACCCGTATGGAGGTTTGTTTGATGATGAACAGAAGAAGGTTTTAGAAATAAAGGAGCAGCTTGAAATTCCTCACCAGCCTGAAGATGCCTTGGTTGAACTGCTTCAAAACTTGGCAGACATGGATATAACATTCCAAGCTCTTAAGGAAACTGATATTGGAAGGCATGTGAACCGGTTGAGGAAGCATTCATCTAATGATGTTCGCAGATTGGTGAAACATCTTGTCAGAAAATGGAAGGAGATTGTGGATGAATGGGTCAGGTTGAATCAGCCTGGGGAGCAGACAGCTACACTCATAGCTGATAGTGACTCACCCCAGCAAAAGGTGCCTCAAAATGGTTATCACCAGGTTCCTGATTTTGCATACTCTCCGAATCCACACAATGGAGGTTCTGGGTCAGATAGAAATAACGTAGAACCCGAACCGAAGGGAAAGTCCGTTCCAAGAAGAGATGCCCCACCAAAACCAGCACAACCGGCTCCAATGTCGTCATATACTCCTCAAAATAGACAGAAAGAACAACAGAAGGAAGCAAATTTTGATTCCCAAAAGCTTGCATCAGCTAGAAAAAGGCTTCAAGAAAATTACAAAGAAGCTGAAAATGCTAAAAAGCAAAGAACAATCCAAGTAATGGACATTCATGAGATCCCAAAGCCAAAAAATACCTTCTTCTCAAAGAACAAAGGCACCGGCGGTGGCTCTCAAGGCAGACATTGGTGA

Protein sequence

MDYDDFAFPPLESTTENEKYKHDFRTILDTAGVDVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPPTRCRNCDTDRYNDGRSNGCEIKQSPVEAKEASPSTPQFVVVEGDDEGVDPYGGLFDDEQKKVLEIKEQLEIPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEIVDEWVRLNQPGEQTATLIADSDSPQQKVPQNGYHQVPDFAYSPNPHNGGSGSDRNNVEPEPKGKSVPRRDAPPKPAQPAPMSSYTPQNRQKEQQKEANFDSQKLASARKRLQENYKEAENAKKQRTIQVMDIHEIPKPKNTFFSKNKGTGGGSQGRHW
Homology
BLAST of Cp4.1LG10g03250.1 vs. ExPASy Swiss-Prot
Match: F4KFC7 (Probable mediator of RNA polymerase II transcription subunit 26c OS=Arabidopsis thaliana OX=3702 GN=MED26C PE=1 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 3.3e-91
Identity = 204/360 (56.67%), Postives = 246/360 (68.33%), Query Frame = 0

Query: 23  DFRTILDTAGVDVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPPTRCRNCD--- 82
           DFR+++D AGVDVWTFIDTA+ VASLDYG +LK RRD IVERLYA TS   +CRNCD   
Sbjct: 5   DFRSVMDNAGVDVWTFIDTAILVASLDYGQELKRRRDNIVERLYA-TSMANKCRNCDFGG 64

Query: 83  --------TDRYNDGR--SNGCEIKQSPVEAKEASPSTPQFVVVEGDDEGVDPYGGLFDD 142
                     R N+GR      E  +  V A        + V VE DD+  DP+ GLFDD
Sbjct: 65  GGNVTEAAIGRVNNGRVHEETEEEDEEGVTAAAEEEVREKSVNVE-DDDDFDPFAGLFDD 124

Query: 143 EQKKVLEIKEQLEIPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVR 202
           EQK ++EIKE+LE P   E++LVELLQNL DMDITFQAL+ETDIGRHVNR+RKH SN+VR
Sbjct: 125 EQKSIVEIKEKLEDPDLSEESLVELLQNLEDMDITFQALQETDIGRHVNRVRKHPSNNVR 184

Query: 203 RLVKHLVRKWKEIVDEWVRLNQPGE-QTATLIADSDSPQQKVPQNG-YHQVPDFAYSPNP 262
           RL K LV+KWKE VDEWV+ NQPG+ +  +LIAD DSP QK   NG   QVPDF YSP P
Sbjct: 185 RLAKQLVKKWKETVDEWVKFNQPGDLEPPSLIADEDSPVQKALHNGSRQQVPDFGYSPVP 244

Query: 263 HNGGSGSDRNN--VEPEPKGKSV---PRRD--APPKPAQPAPMSSYTPQNRQKEQQKEAN 322
            NG S S +N+   EPE K + V   PRR+  +P KP++P+P     P+++   + KE +
Sbjct: 245 QNGYSSSSKNSNITEPERKPRPVAPQPRRESPSPAKPSRPSPSQQTIPRDK---EHKEVD 304

Query: 323 FDSQKLASARKRLQENYKEAENAKKQRTIQVMDIHEIPKPKNTFFSKNKGTGGGSQGRHW 361
           FD     +ARKRLQ+NY++AENAKKQRTIQVMDIH+IPKPK   F   KG G    GRHW
Sbjct: 305 FD-----TARKRLQQNYRQAENAKKQRTIQVMDIHDIPKPKKGGFFPRKG-GSSQGGRHW 353

BLAST of Cp4.1LG10g03250.1 vs. ExPASy Swiss-Prot
Match: Q9FHK9 (Probable mediator of RNA polymerase II transcription subunit 26b OS=Arabidopsis thaliana OX=3702 GN=MED26B PE=2 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 2.1e-16
Identity = 112/417 (26.86%), Postives = 171/417 (41.01%), Query Frame = 0

Query: 34  DVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPPTRCRNCD-----------TDR 93
           D++  ID A+ VA+ D  N+ K+RRD I E L++      RC  CD            +R
Sbjct: 21  DIFGIIDHAIMVAATDCPNKFKSRRDKIAELLFSCR--VNRCVGCDHLELSVPGDDEANR 80

Query: 94  YNDGRSNG-------CEIKQSPVEAKEASPSTPQFVVVEG----DDEGVDPYGGLFDDEQ 153
              G   G        E+     E+K  S       +V      + E +      F    
Sbjct: 81  GTTGNGGGGTAVDEDYEVAGGSKESKANSSRGDNNQIVSNYTFDEAEALSDEIEEFSVVS 140

Query: 154 KKVLEIKE-QLEIPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRR 213
           K+V  IKE  L    +P   L++ L++L  M +    LK T+IG+ VN LRKHSS+ +R+
Sbjct: 141 KEVARIKEILLNKEDEPNSVLLDSLRHLKLMSLNVDILKSTEIGKAVNGLRKHSSDKIRQ 200

Query: 214 LVKHLVRKWKEIVDEWVRLNQ-------PGEQTATLIADSDSPQQKVP---------QNG 273
           L K L+ +WKE+VD+WV   +         E     + D +     +P          NG
Sbjct: 201 LAKTLIAEWKELVDQWVNTTKEITGAEGTPESANPSVLDEEEAFPSLPYDVDIFTPEPNG 260

Query: 274 Y---HQVPDFAYSPNP-----HNGGSGSDR---NNVEPEPKGKSVPRRDAPPKPAQPA-- 333
           +   H      +  NP     HN     +R   N  + +P+G  +  +DAP +  +P+  
Sbjct: 261 FEISHFFDSLDFDGNPRNSEEHNTSREHERRPQNIAKRKPEGTQMRIQDAPFRSIKPSSA 320

Query: 334 --------PMSSYTPQNRQKE-----------------------------QQKEANFDSQ 361
                   P+   T Q  + E                             Q+K    D+ 
Sbjct: 321 TDFDGTRRPVKQSTEQRMKNETVSVHKSEKPMIQRKPVVTEQKRKAPGPQQEKLKGLDAD 380

BLAST of Cp4.1LG10g03250.1 vs. ExPASy Swiss-Prot
Match: F4J4Y5 (Probable mediator of RNA polymerase II transcription subunit 26a OS=Arabidopsis thaliana OX=3702 GN=MED26A PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 4.5e-11
Identity = 96/382 (25.13%), Postives = 163/382 (42.67%), Query Frame = 0

Query: 34  DVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPPTRCRNCDTDRYNDGRSNGCEI 93
           D++  ID A+ VA+ D+  + K+R D I E L++       C+          R  GC+ 
Sbjct: 23  DIFGIIDHAIMVAAADWPKEFKSRSDRIAELLFS-------CK--------VSRCIGCDH 82

Query: 94  KQSPVEAKEASPSTPQFVVVEGDDEGVDPYGGLFDDEQ-----KKVLEIKEQLEIPHQPE 153
            +  +   EA+    + V V G  +  D      + E+      +V+ I++ L      +
Sbjct: 83  LELSIAGDEAA---VEIVGVGGGGDRGDSGVATGEGEEASVSVDEVMRIRDILSNKDDEK 142

Query: 154 DA-LVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEIVDEWV 213
           D+ L+E L+ L  M ++   LK+T+IG+ VN LR+HSS+ + +L K L  +WK +VD+W 
Sbjct: 143 DSVLLESLRKLESMSMSVDILKDTEIGKAVNGLRRHSSDKISKLAKTLFAEWKRLVDQW- 202

Query: 214 RLNQPGEQTAT---------LIADSDSPQQKVPQNGYHQVPDFAYSPNPHN--------G 273
            +N P E   T          + D +      P    H +  +A  PN            
Sbjct: 203 -MNTPEEMAGTEGTPESLNLSVIDEEEAFPSPP----HDLDIYAPEPNGFELSQILDCLD 262

Query: 274 GSGSDRNNVEPE------------PKG-------------KSVPRRDAPPKPAQPAPMSS 333
             G+ R++VE +            PKG             +   R +A  +P + +    
Sbjct: 263 CDGNPRHSVESKHERKSQSSAGRRPKGTNDANVVGRYCNDQQTRREEADVRPMKHSATDV 322

Query: 334 YTPQNRQKEQQKE-----------ANFDSQKLAS-----------------ARKRLQENY 340
             P+ + K+ +++                +KLA                  A+++LQE+Y
Sbjct: 323 VEPKRQTKQSREQMVSAIQRKPTAVTEQKRKLAGPQQDKLKALDPDSKFEFAKRKLQESY 378

BLAST of Cp4.1LG10g03250.1 vs. ExPASy Swiss-Prot
Match: Q96MN5 (Transcription elongation factor A N-terminal and central domain-containing protein 2 OS=Homo sapiens OX=9606 GN=TCEANC2 PE=1 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 3.7e-05
Identity = 26/68 (38.24%), Postives = 40/68 (58.82%), Query Frame = 0

Query: 138 KEQLEIPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVR 197
           K  LE+P Q ++ LVE LQ L     + + LK T IG  VN++RKHS ++V  L + +  
Sbjct: 46  KTMLELPDQTKENLVEALQELKKKIPSREVLKSTRIGHTVNKMRKHSDSEVASLAREVYT 105

Query: 198 KWKEIVDE 206
           +WK   ++
Sbjct: 106 EWKTFTEK 113

BLAST of Cp4.1LG10g03250.1 vs. ExPASy Swiss-Prot
Match: B0UYI1 (Transcription elongation factor A N-terminal and central domain-containing protein 2 OS=Danio rerio OX=7955 GN=tceanc2 PE=3 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 1.4e-04
Identity = 25/69 (36.23%), Postives = 42/69 (60.87%), Query Frame = 0

Query: 138 KEQLEIPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSN-DVRRLVKHLV 197
           K  LE+P QP++ ++E L+ L     + + LK T IG  VN LRKHS + +++ L K + 
Sbjct: 45  KSILELPGQPKENMIEALKELDKKVPSREVLKSTRIGHTVNNLRKHSDDPEIKSLAKEVY 104

Query: 198 RKWKEIVDE 206
           + W+  ++E
Sbjct: 105 KHWRTFIEE 113

BLAST of Cp4.1LG10g03250.1 vs. NCBI nr
Match: XP_023544969.1 (probable mediator of RNA polymerase II transcription subunit 26c [Cucurbita pepo subsp. pepo])

HSP 1 Score: 672 bits (1733), Expect = 5.82e-243
Identity = 338/338 (100.00%), Postives = 338/338 (100.00%), Query Frame = 0

Query: 23  DFRTILDTAGVDVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPPTRCRNCDTDR 82
           DFRTILDTAGVDVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPPTRCRNCDTDR
Sbjct: 8   DFRTILDTAGVDVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPPTRCRNCDTDR 67

Query: 83  YNDGRSNGCEIKQSPVEAKEASPSTPQFVVVEGDDEGVDPYGGLFDDEQKKVLEIKEQLE 142
           YNDGRSNGCEIKQSPVEAKEASPSTPQFVVVEGDDEGVDPYGGLFDDEQKKVLEIKEQLE
Sbjct: 68  YNDGRSNGCEIKQSPVEAKEASPSTPQFVVVEGDDEGVDPYGGLFDDEQKKVLEIKEQLE 127

Query: 143 IPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEI 202
           IPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEI
Sbjct: 128 IPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEI 187

Query: 203 VDEWVRLNQPGEQTATLIADSDSPQQKVPQNGYHQVPDFAYSPNPHNGGSGSDRNNVEPE 262
           VDEWVRLNQPGEQTATLIADSDSPQQKVPQNGYHQVPDFAYSPNPHNGGSGSDRNNVEPE
Sbjct: 188 VDEWVRLNQPGEQTATLIADSDSPQQKVPQNGYHQVPDFAYSPNPHNGGSGSDRNNVEPE 247

Query: 263 PKGKSVPRRDAPPKPAQPAPMSSYTPQNRQKEQQKEANFDSQKLASARKRLQENYKEAEN 322
           PKGKSVPRRDAPPKPAQPAPMSSYTPQNRQKEQQKEANFDSQKLASARKRLQENYKEAEN
Sbjct: 248 PKGKSVPRRDAPPKPAQPAPMSSYTPQNRQKEQQKEANFDSQKLASARKRLQENYKEAEN 307

Query: 323 AKKQRTIQVMDIHEIPKPKNTFFSKNKGTGGGSQGRHW 360
           AKKQRTIQVMDIHEIPKPKNTFFSKNKGTGGGSQGRHW
Sbjct: 308 AKKQRTIQVMDIHEIPKPKNTFFSKNKGTGGGSQGRHW 345

BLAST of Cp4.1LG10g03250.1 vs. NCBI nr
Match: XP_022950583.1 (probable mediator of RNA polymerase II transcription subunit 26c [Cucurbita moschata] >KAG7034423.1 putative mediator of RNA polymerase II transcription subunit 26c, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 666 bits (1719), Expect = 7.92e-241
Identity = 335/338 (99.11%), Postives = 336/338 (99.41%), Query Frame = 0

Query: 23  DFRTILDTAGVDVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPPTRCRNCDTDR 82
           DFRTILDTAGVDVWTFIDTAMEVAS+DYGNQLKNRRDGIVERLYALTSPPTRCRNCDTDR
Sbjct: 8   DFRTILDTAGVDVWTFIDTAMEVASMDYGNQLKNRRDGIVERLYALTSPPTRCRNCDTDR 67

Query: 83  YNDGRSNGCEIKQSPVEAKEASPSTPQFVVVEGDDEGVDPYGGLFDDEQKKVLEIKEQLE 142
           YNDGRSNGCEIKQSPVEAKEASPSTPQFVVVEGDDEGVDPYGGLFDDEQKKVLEIKEQLE
Sbjct: 68  YNDGRSNGCEIKQSPVEAKEASPSTPQFVVVEGDDEGVDPYGGLFDDEQKKVLEIKEQLE 127

Query: 143 IPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEI 202
           IPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEI
Sbjct: 128 IPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEI 187

Query: 203 VDEWVRLNQPGEQTATLIADSDSPQQKVPQNGYHQVPDFAYSPNPHNGGSGSDRNNVEPE 262
           VDEWVRLNQPGEQTATLIAD DSPQQKV QNGYHQVPDFAYSPNPHNGGSGSDRNNVEPE
Sbjct: 188 VDEWVRLNQPGEQTATLIADGDSPQQKVTQNGYHQVPDFAYSPNPHNGGSGSDRNNVEPE 247

Query: 263 PKGKSVPRRDAPPKPAQPAPMSSYTPQNRQKEQQKEANFDSQKLASARKRLQENYKEAEN 322
           PKGKSVPRRDAPPKPAQPAPMSSYTPQNRQKEQQKEANFDSQKLASARKRLQENYKEAEN
Sbjct: 248 PKGKSVPRRDAPPKPAQPAPMSSYTPQNRQKEQQKEANFDSQKLASARKRLQENYKEAEN 307

Query: 323 AKKQRTIQVMDIHEIPKPKNTFFSKNKGTGGGSQGRHW 360
           AKKQRTIQVMDIHEIPKPKNTFFSKNKGTGGGSQGRHW
Sbjct: 308 AKKQRTIQVMDIHEIPKPKNTFFSKNKGTGGGSQGRHW 345

BLAST of Cp4.1LG10g03250.1 vs. NCBI nr
Match: KAG6604266.1 (putative mediator of RNA polymerase II transcription subunit 26c, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 665 bits (1717), Expect = 1.60e-240
Identity = 335/338 (99.11%), Postives = 336/338 (99.41%), Query Frame = 0

Query: 23  DFRTILDTAGVDVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPPTRCRNCDTDR 82
           DFRTIL+TAGVDVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPPTRCRNCDTDR
Sbjct: 8   DFRTILETAGVDVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPPTRCRNCDTDR 67

Query: 83  YNDGRSNGCEIKQSPVEAKEASPSTPQFVVVEGDDEGVDPYGGLFDDEQKKVLEIKEQLE 142
           YNDGRSNGCEIKQSPVEAKEASPSTPQFVVVEGDDEGVDPYGGLFDDEQKKVLEIKEQLE
Sbjct: 68  YNDGRSNGCEIKQSPVEAKEASPSTPQFVVVEGDDEGVDPYGGLFDDEQKKVLEIKEQLE 127

Query: 143 IPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEI 202
           IPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEI
Sbjct: 128 IPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEI 187

Query: 203 VDEWVRLNQPGEQTATLIADSDSPQQKVPQNGYHQVPDFAYSPNPHNGGSGSDRNNVEPE 262
           VDEWVRLNQPGEQTATLIAD DSPQQKV QNGYHQVPDFAYSPNPHNGGSGSDRNNVEPE
Sbjct: 188 VDEWVRLNQPGEQTATLIADGDSPQQKVTQNGYHQVPDFAYSPNPHNGGSGSDRNNVEPE 247

Query: 263 PKGKSVPRRDAPPKPAQPAPMSSYTPQNRQKEQQKEANFDSQKLASARKRLQENYKEAEN 322
           PKGKSVPRRDAPPKPAQPAPMSSYTPQNRQKEQQKEANFDSQKLASARKRLQENYKEAEN
Sbjct: 248 PKGKSVPRRDAPPKPAQPAPMSSYTPQNRQKEQQKEANFDSQKLASARKRLQENYKEAEN 307

Query: 323 AKKQRTIQVMDIHEIPKPKNTFFSKNKGTGGGSQGRHW 360
           AKKQRTIQVMDIHEIPKPKNTFFSKNKGTGGGSQGRHW
Sbjct: 308 AKKQRTIQVMDIHEIPKPKNTFFSKNKGTGGGSQGRHW 345

BLAST of Cp4.1LG10g03250.1 vs. NCBI nr
Match: XP_022977926.1 (probable mediator of RNA polymerase II transcription subunit 26c [Cucurbita maxima])

HSP 1 Score: 659 bits (1699), Expect = 9.19e-238
Identity = 335/339 (98.82%), Postives = 335/339 (98.82%), Query Frame = 0

Query: 23  DFRTILDTAGVDVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPPTRCRNCDTDR 82
           DFRTILDTAGVDVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPPTRCRNCDTDR
Sbjct: 8   DFRTILDTAGVDVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPPTRCRNCDTDR 67

Query: 83  YNDGRSNGCEIKQSPVEAKEASPSTPQFVVVEGDDEGVDPYGGLFDDEQKKVLEIKEQLE 142
           YNDGRSNGCEIKQSPVEAKEASPSTPQFVVVEGDDEGVDPYGGLFDDEQKKVLEIKEQLE
Sbjct: 68  YNDGRSNGCEIKQSPVEAKEASPSTPQFVVVEGDDEGVDPYGGLFDDEQKKVLEIKEQLE 127

Query: 143 IPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEI 202
           IPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEI
Sbjct: 128 IPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEI 187

Query: 203 VDEWVRLNQPGEQTATLIADSDSPQQKVPQNGYHQVPDFAYSPNPHNGGSGSDRNNVEPE 262
           VDEWVRLNQPGEQTATLIAD DSPQQKV QNGYHQVPDFAYSPNPHNGGSGSDRNNVEPE
Sbjct: 188 VDEWVRLNQPGEQTATLIADGDSPQQKVSQNGYHQVPDFAYSPNPHNGGSGSDRNNVEPE 247

Query: 263 PKGKSVPRRDAPPKPAQPAPMSS-YTPQNRQKEQQKEANFDSQKLASARKRLQENYKEAE 322
           PKGKSVPRRDA PKPAQPAPMSS YTPQNRQKEQQKEANFDSQKLASARKRLQENYKEAE
Sbjct: 248 PKGKSVPRRDALPKPAQPAPMSSSYTPQNRQKEQQKEANFDSQKLASARKRLQENYKEAE 307

Query: 323 NAKKQRTIQVMDIHEIPKPKNTFFSKNKGTGGGSQGRHW 360
           NAKKQRTIQVMDIHEIPKPKNTFFSKNKGTGGGSQGRHW
Sbjct: 308 NAKKQRTIQVMDIHEIPKPKNTFFSKNKGTGGGSQGRHW 346

BLAST of Cp4.1LG10g03250.1 vs. NCBI nr
Match: XP_038882890.1 (probable mediator of RNA polymerase II transcription subunit 26c [Benincasa hispida])

HSP 1 Score: 634 bits (1636), Expect = 3.53e-228
Identity = 318/338 (94.08%), Postives = 326/338 (96.45%), Query Frame = 0

Query: 23  DFRTILDTAGVDVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPPTRCRNCDTDR 82
           DFRTILDTAGVDVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPP+RCRNCDTDR
Sbjct: 8   DFRTILDTAGVDVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPPSRCRNCDTDR 67

Query: 83  YNDGRSNGCEIKQSPVEAKEASPSTPQFVVVEGDDEGVDPYGGLFDDEQKKVLEIKEQLE 142
            +DGRSNGCEIKQSP E K ASPSTPQFV V+GDD+GVDP+GGLFDDEQKKVLEIKEQLE
Sbjct: 68  NHDGRSNGCEIKQSPGEVKGASPSTPQFVAVDGDDDGVDPHGGLFDDEQKKVLEIKEQLE 127

Query: 143 IPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEI 202
           IPHQPED LVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEI
Sbjct: 128 IPHQPEDGLVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEI 187

Query: 203 VDEWVRLNQPGEQTATLIADSDSPQQKVPQNGYHQVPDFAYSPNPHNGGSGSDRNNVEPE 262
           VDEWVRLNQPGEQTATL+AD DSPQQK PQNGYHQVPDFAYSPNPHNGGSGSDRNN EPE
Sbjct: 188 VDEWVRLNQPGEQTATLLADGDSPQQKAPQNGYHQVPDFAYSPNPHNGGSGSDRNNSEPE 247

Query: 263 PKGKSVPRRDAPPKPAQPAPMSSYTPQNRQKEQQKEANFDSQKLASARKRLQENYKEAEN 322
           PKGKSVPRRDAPPKPAQPAP SS TPQNRQKEQQKEANFDSQKLASARKRLQENYKEAEN
Sbjct: 248 PKGKSVPRRDAPPKPAQPAPTSSLTPQNRQKEQQKEANFDSQKLASARKRLQENYKEAEN 307

Query: 323 AKKQRTIQVMDIHEIPKPKNTFFSKNKGTGGGSQGRHW 360
           AK+QRTIQVMDIHEIPKPKN FFSKNKG+GGGSQGRHW
Sbjct: 308 AKRQRTIQVMDIHEIPKPKNAFFSKNKGSGGGSQGRHW 345

BLAST of Cp4.1LG10g03250.1 vs. ExPASy TrEMBL
Match: A0A6J1GFA3 (probable mediator of RNA polymerase II transcription subunit 26c OS=Cucurbita moschata OX=3662 GN=LOC111453638 PE=4 SV=1)

HSP 1 Score: 666 bits (1719), Expect = 3.84e-241
Identity = 335/338 (99.11%), Postives = 336/338 (99.41%), Query Frame = 0

Query: 23  DFRTILDTAGVDVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPPTRCRNCDTDR 82
           DFRTILDTAGVDVWTFIDTAMEVAS+DYGNQLKNRRDGIVERLYALTSPPTRCRNCDTDR
Sbjct: 8   DFRTILDTAGVDVWTFIDTAMEVASMDYGNQLKNRRDGIVERLYALTSPPTRCRNCDTDR 67

Query: 83  YNDGRSNGCEIKQSPVEAKEASPSTPQFVVVEGDDEGVDPYGGLFDDEQKKVLEIKEQLE 142
           YNDGRSNGCEIKQSPVEAKEASPSTPQFVVVEGDDEGVDPYGGLFDDEQKKVLEIKEQLE
Sbjct: 68  YNDGRSNGCEIKQSPVEAKEASPSTPQFVVVEGDDEGVDPYGGLFDDEQKKVLEIKEQLE 127

Query: 143 IPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEI 202
           IPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEI
Sbjct: 128 IPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEI 187

Query: 203 VDEWVRLNQPGEQTATLIADSDSPQQKVPQNGYHQVPDFAYSPNPHNGGSGSDRNNVEPE 262
           VDEWVRLNQPGEQTATLIAD DSPQQKV QNGYHQVPDFAYSPNPHNGGSGSDRNNVEPE
Sbjct: 188 VDEWVRLNQPGEQTATLIADGDSPQQKVTQNGYHQVPDFAYSPNPHNGGSGSDRNNVEPE 247

Query: 263 PKGKSVPRRDAPPKPAQPAPMSSYTPQNRQKEQQKEANFDSQKLASARKRLQENYKEAEN 322
           PKGKSVPRRDAPPKPAQPAPMSSYTPQNRQKEQQKEANFDSQKLASARKRLQENYKEAEN
Sbjct: 248 PKGKSVPRRDAPPKPAQPAPMSSYTPQNRQKEQQKEANFDSQKLASARKRLQENYKEAEN 307

Query: 323 AKKQRTIQVMDIHEIPKPKNTFFSKNKGTGGGSQGRHW 360
           AKKQRTIQVMDIHEIPKPKNTFFSKNKGTGGGSQGRHW
Sbjct: 308 AKKQRTIQVMDIHEIPKPKNTFFSKNKGTGGGSQGRHW 345

BLAST of Cp4.1LG10g03250.1 vs. ExPASy TrEMBL
Match: A0A6J1ILB4 (probable mediator of RNA polymerase II transcription subunit 26c OS=Cucurbita maxima OX=3661 GN=LOC111478070 PE=4 SV=1)

HSP 1 Score: 659 bits (1699), Expect = 4.45e-238
Identity = 335/339 (98.82%), Postives = 335/339 (98.82%), Query Frame = 0

Query: 23  DFRTILDTAGVDVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPPTRCRNCDTDR 82
           DFRTILDTAGVDVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPPTRCRNCDTDR
Sbjct: 8   DFRTILDTAGVDVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPPTRCRNCDTDR 67

Query: 83  YNDGRSNGCEIKQSPVEAKEASPSTPQFVVVEGDDEGVDPYGGLFDDEQKKVLEIKEQLE 142
           YNDGRSNGCEIKQSPVEAKEASPSTPQFVVVEGDDEGVDPYGGLFDDEQKKVLEIKEQLE
Sbjct: 68  YNDGRSNGCEIKQSPVEAKEASPSTPQFVVVEGDDEGVDPYGGLFDDEQKKVLEIKEQLE 127

Query: 143 IPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEI 202
           IPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEI
Sbjct: 128 IPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEI 187

Query: 203 VDEWVRLNQPGEQTATLIADSDSPQQKVPQNGYHQVPDFAYSPNPHNGGSGSDRNNVEPE 262
           VDEWVRLNQPGEQTATLIAD DSPQQKV QNGYHQVPDFAYSPNPHNGGSGSDRNNVEPE
Sbjct: 188 VDEWVRLNQPGEQTATLIADGDSPQQKVSQNGYHQVPDFAYSPNPHNGGSGSDRNNVEPE 247

Query: 263 PKGKSVPRRDAPPKPAQPAPMSS-YTPQNRQKEQQKEANFDSQKLASARKRLQENYKEAE 322
           PKGKSVPRRDA PKPAQPAPMSS YTPQNRQKEQQKEANFDSQKLASARKRLQENYKEAE
Sbjct: 248 PKGKSVPRRDALPKPAQPAPMSSSYTPQNRQKEQQKEANFDSQKLASARKRLQENYKEAE 307

Query: 323 NAKKQRTIQVMDIHEIPKPKNTFFSKNKGTGGGSQGRHW 360
           NAKKQRTIQVMDIHEIPKPKNTFFSKNKGTGGGSQGRHW
Sbjct: 308 NAKKQRTIQVMDIHEIPKPKNTFFSKNKGTGGGSQGRHW 346

BLAST of Cp4.1LG10g03250.1 vs. ExPASy TrEMBL
Match: A0A5A7SIU9 (Putative mediator of RNA polymerase II transcription subunit 26c OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00130 PE=4 SV=1)

HSP 1 Score: 627 bits (1618), Expect = 9.44e-226
Identity = 316/338 (93.49%), Postives = 322/338 (95.27%), Query Frame = 0

Query: 23  DFRTILDTAGVDVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPPTRCRNCDTDR 82
           DFRTILDTAGVDVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPP+RCRNCDTDR
Sbjct: 8   DFRTILDTAGVDVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPPSRCRNCDTDR 67

Query: 83  YNDGRSNGCEIKQSPVEAKEASPSTPQFVVVEGDDEGVDPYGGLFDDEQKKVLEIKEQLE 142
            +DGRSNGCEIKQ   E K ASPSTPQFVVVEGDD+G DPY GLFDDEQKKVLEIKEQLE
Sbjct: 68  NHDGRSNGCEIKQGSGEVKGASPSTPQFVVVEGDDDGADPYAGLFDDEQKKVLEIKEQLE 127

Query: 143 IPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEI 202
           IPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEI
Sbjct: 128 IPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEI 187

Query: 203 VDEWVRLNQPGEQTATLIADSDSPQQKVPQNGYHQVPDFAYSPNPHNGGSGSDRNNVEPE 262
           VDEWVRLNQPGEQTATL+AD DSPQQK PQNGYHQVPDFAYSPNPHNGGSGSDRNN EPE
Sbjct: 188 VDEWVRLNQPGEQTATLLADGDSPQQKAPQNGYHQVPDFAYSPNPHNGGSGSDRNNSEPE 247

Query: 263 PKGKSVPRRDAPPKPAQPAPMSSYTPQNRQKEQQKEANFDSQKLASARKRLQENYKEAEN 322
           PKGKSVPRRDAPPKP Q AP SS TPQNRQKEQQKEANFDSQKLASARKRLQENYKEAEN
Sbjct: 248 PKGKSVPRRDAPPKPTQQAPTSSLTPQNRQKEQQKEANFDSQKLASARKRLQENYKEAEN 307

Query: 323 AKKQRTIQVMDIHEIPKPKNTFFSKNKGTGGGSQGRHW 360
           AK+QRTIQVMDIHEIPKPKN FFSKNKG+GGGSQGRHW
Sbjct: 308 AKRQRTIQVMDIHEIPKPKNAFFSKNKGSGGGSQGRHW 345

BLAST of Cp4.1LG10g03250.1 vs. ExPASy TrEMBL
Match: A0A1S3B2G2 (probable mediator of RNA polymerase II transcription subunit 26c OS=Cucumis melo OX=3656 GN=LOC103485244 PE=4 SV=1)

HSP 1 Score: 627 bits (1618), Expect = 9.44e-226
Identity = 316/338 (93.49%), Postives = 322/338 (95.27%), Query Frame = 0

Query: 23  DFRTILDTAGVDVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPPTRCRNCDTDR 82
           DFRTILDTAGVDVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPP+RCRNCDTDR
Sbjct: 8   DFRTILDTAGVDVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPPSRCRNCDTDR 67

Query: 83  YNDGRSNGCEIKQSPVEAKEASPSTPQFVVVEGDDEGVDPYGGLFDDEQKKVLEIKEQLE 142
            +DGRSNGCEIKQ   E K ASPSTPQFVVVEGDD+G DPY GLFDDEQKKVLEIKEQLE
Sbjct: 68  NHDGRSNGCEIKQGSGEVKGASPSTPQFVVVEGDDDGADPYAGLFDDEQKKVLEIKEQLE 127

Query: 143 IPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEI 202
           IPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEI
Sbjct: 128 IPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEI 187

Query: 203 VDEWVRLNQPGEQTATLIADSDSPQQKVPQNGYHQVPDFAYSPNPHNGGSGSDRNNVEPE 262
           VDEWVRLNQPGEQTATL+AD DSPQQK PQNGYHQVPDFAYSPNPHNGGSGSDRNN EPE
Sbjct: 188 VDEWVRLNQPGEQTATLLADGDSPQQKAPQNGYHQVPDFAYSPNPHNGGSGSDRNNSEPE 247

Query: 263 PKGKSVPRRDAPPKPAQPAPMSSYTPQNRQKEQQKEANFDSQKLASARKRLQENYKEAEN 322
           PKGKSVPRRDAPPKP Q AP SS TPQNRQKEQQKEANFDSQKLASARKRLQENYKEAEN
Sbjct: 248 PKGKSVPRRDAPPKPTQQAPTSSLTPQNRQKEQQKEANFDSQKLASARKRLQENYKEAEN 307

Query: 323 AKKQRTIQVMDIHEIPKPKNTFFSKNKGTGGGSQGRHW 360
           AK+QRTIQVMDIHEIPKPKN FFSKNKG+GGGSQGRHW
Sbjct: 308 AKRQRTIQVMDIHEIPKPKNAFFSKNKGSGGGSQGRHW 345

BLAST of Cp4.1LG10g03250.1 vs. ExPASy TrEMBL
Match: A0A0A0KHV9 (TFIIS N-terminal domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G486670 PE=4 SV=1)

HSP 1 Score: 621 bits (1602), Expect = 2.59e-223
Identity = 314/338 (92.90%), Postives = 320/338 (94.67%), Query Frame = 0

Query: 23  DFRTILDTAGVDVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPPTRCRNCDTDR 82
           DFRTILDTAGVDVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPP+RCRNCDTDR
Sbjct: 8   DFRTILDTAGVDVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPPSRCRNCDTDR 67

Query: 83  YNDGRSNGCEIKQSPVEAKEASPSTPQFVVVEGDDEGVDPYGGLFDDEQKKVLEIKEQLE 142
            +DGRSNGCEIKQ   E KEASPSTPQFVVVEGDD+G DPY GLFDDEQKKVLEIKEQLE
Sbjct: 68  NHDGRSNGCEIKQGSGEVKEASPSTPQFVVVEGDDDGADPYAGLFDDEQKKVLEIKEQLE 127

Query: 143 IPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEI 202
           IP QPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKH SNDVRRLVKHLVRKWKEI
Sbjct: 128 IPQQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHPSNDVRRLVKHLVRKWKEI 187

Query: 203 VDEWVRLNQPGEQTATLIADSDSPQQKVPQNGYHQVPDFAYSPNPHNGGSGSDRNNVEPE 262
           VDEWVRLNQPGEQTATL+AD DSPQQK PQNGYHQVPDFAYSPNPHNGGSGSDRNN EPE
Sbjct: 188 VDEWVRLNQPGEQTATLLADGDSPQQKAPQNGYHQVPDFAYSPNPHNGGSGSDRNNSEPE 247

Query: 263 PKGKSVPRRDAPPKPAQPAPMSSYTPQNRQKEQQKEANFDSQKLASARKRLQENYKEAEN 322
           PKGKSVPRRDA PKP Q AP SS TPQNRQKEQQKEANFDSQKLASARKRLQENYKEAEN
Sbjct: 248 PKGKSVPRRDALPKPTQQAPTSSLTPQNRQKEQQKEANFDSQKLASARKRLQENYKEAEN 307

Query: 323 AKKQRTIQVMDIHEIPKPKNTFFSKNKGTGGGSQGRHW 360
           AK+QRTIQVMDIHEIPKPKN FFSKNKG+GGGSQGRHW
Sbjct: 308 AKRQRTIQVMDIHEIPKPKNAFFSKNKGSGGGSQGRHW 345

BLAST of Cp4.1LG10g03250.1 vs. TAIR 10
Match: AT5G09850.1 (Transcription elongation factor (TFIIS) family protein )

HSP 1 Score: 336.7 bits (862), Expect = 2.4e-92
Identity = 204/360 (56.67%), Postives = 246/360 (68.33%), Query Frame = 0

Query: 23  DFRTILDTAGVDVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPPTRCRNCD--- 82
           DFR+++D AGVDVWTFIDTA+ VASLDYG +LK RRD IVERLYA TS   +CRNCD   
Sbjct: 5   DFRSVMDNAGVDVWTFIDTAILVASLDYGQELKRRRDNIVERLYA-TSMANKCRNCDFGG 64

Query: 83  --------TDRYNDGR--SNGCEIKQSPVEAKEASPSTPQFVVVEGDDEGVDPYGGLFDD 142
                     R N+GR      E  +  V A        + V VE DD+  DP+ GLFDD
Sbjct: 65  GGNVTEAAIGRVNNGRVHEETEEEDEEGVTAAAEEEVREKSVNVE-DDDDFDPFAGLFDD 124

Query: 143 EQKKVLEIKEQLEIPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVR 202
           EQK ++EIKE+LE P   E++LVELLQNL DMDITFQAL+ETDIGRHVNR+RKH SN+VR
Sbjct: 125 EQKSIVEIKEKLEDPDLSEESLVELLQNLEDMDITFQALQETDIGRHVNRVRKHPSNNVR 184

Query: 203 RLVKHLVRKWKEIVDEWVRLNQPGE-QTATLIADSDSPQQKVPQNG-YHQVPDFAYSPNP 262
           RL K LV+KWKE VDEWV+ NQPG+ +  +LIAD DSP QK   NG   QVPDF YSP P
Sbjct: 185 RLAKQLVKKWKETVDEWVKFNQPGDLEPPSLIADEDSPVQKALHNGSRQQVPDFGYSPVP 244

Query: 263 HNGGSGSDRNN--VEPEPKGKSV---PRRD--APPKPAQPAPMSSYTPQNRQKEQQKEAN 322
            NG S S +N+   EPE K + V   PRR+  +P KP++P+P     P+++   + KE +
Sbjct: 245 QNGYSSSSKNSNITEPERKPRPVAPQPRRESPSPAKPSRPSPSQQTIPRDK---EHKEVD 304

Query: 323 FDSQKLASARKRLQENYKEAENAKKQRTIQVMDIHEIPKPKNTFFSKNKGTGGGSQGRHW 361
           FD     +ARKRLQ+NY++AENAKKQRTIQVMDIH+IPKPK   F   KG G    GRHW
Sbjct: 305 FD-----TARKRLQQNYRQAENAKKQRTIQVMDIHDIPKPKKGGFFPRKG-GSSQGGRHW 353

BLAST of Cp4.1LG10g03250.1 vs. TAIR 10
Match: AT5G05140.1 (Transcription elongation factor (TFIIS) family protein )

HSP 1 Score: 88.2 bits (217), Expect = 1.5e-17
Identity = 112/417 (26.86%), Postives = 171/417 (41.01%), Query Frame = 0

Query: 34  DVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPPTRCRNCD-----------TDR 93
           D++  ID A+ VA+ D  N+ K+RRD I E L++      RC  CD            +R
Sbjct: 21  DIFGIIDHAIMVAATDCPNKFKSRRDKIAELLFSCR--VNRCVGCDHLELSVPGDDEANR 80

Query: 94  YNDGRSNG-------CEIKQSPVEAKEASPSTPQFVVVEG----DDEGVDPYGGLFDDEQ 153
              G   G        E+     E+K  S       +V      + E +      F    
Sbjct: 81  GTTGNGGGGTAVDEDYEVAGGSKESKANSSRGDNNQIVSNYTFDEAEALSDEIEEFSVVS 140

Query: 154 KKVLEIKE-QLEIPHQPEDALVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRR 213
           K+V  IKE  L    +P   L++ L++L  M +    LK T+IG+ VN LRKHSS+ +R+
Sbjct: 141 KEVARIKEILLNKEDEPNSVLLDSLRHLKLMSLNVDILKSTEIGKAVNGLRKHSSDKIRQ 200

Query: 214 LVKHLVRKWKEIVDEWVRLNQ-------PGEQTATLIADSDSPQQKVP---------QNG 273
           L K L+ +WKE+VD+WV   +         E     + D +     +P          NG
Sbjct: 201 LAKTLIAEWKELVDQWVNTTKEITGAEGTPESANPSVLDEEEAFPSLPYDVDIFTPEPNG 260

Query: 274 Y---HQVPDFAYSPNP-----HNGGSGSDR---NNVEPEPKGKSVPRRDAPPKPAQPA-- 333
           +   H      +  NP     HN     +R   N  + +P+G  +  +DAP +  +P+  
Sbjct: 261 FEISHFFDSLDFDGNPRNSEEHNTSREHERRPQNIAKRKPEGTQMRIQDAPFRSIKPSSA 320

Query: 334 --------PMSSYTPQNRQKE-----------------------------QQKEANFDSQ 361
                   P+   T Q  + E                             Q+K    D+ 
Sbjct: 321 TDFDGTRRPVKQSTEQRMKNETVSVHKSEKPMIQRKPVVTEQKRKAPGPQQEKLKGLDAD 380

BLAST of Cp4.1LG10g03250.1 vs. TAIR 10
Match: AT3G10820.1 (Transcription elongation factor (TFIIS) family protein )

HSP 1 Score: 73.9 bits (180), Expect = 2.9e-13
Identity = 57/191 (29.84%), Postives = 96/191 (50.26%), Query Frame = 0

Query: 34  DVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPPTRCRNCDTDRYNDGRSNGCEI 93
           D++  ID A+ VA+ D+  + K+R D I E L++       C+          R  GC+ 
Sbjct: 23  DIFGIIDHAIMVAAADWPKEFKSRSDRIAELLFS-------CK--------VSRCIGCDH 82

Query: 94  KQSPVEAKEASPSTPQFVVVEGDDEGVDPYGGLFDDEQ-----KKVLEIKEQLEIPHQPE 153
            +  +   EA+    + V V G  +  D      + E+      +V+ I++ L      +
Sbjct: 83  LELSIAGDEAA---VEIVGVGGGGDRGDSGVATGEGEEASVSVDEVMRIRDILSNKDDEK 142

Query: 154 DA-LVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEIVDEWV 213
           D+ L+E L+ L  M ++   LK+T+IG+ VN LR+HSS+ + +L K L  +WK +VD+W 
Sbjct: 143 DSVLLESLRKLESMSMSVDILKDTEIGKAVNGLRRHSSDKISKLAKTLFAEWKRLVDQW- 193

Query: 214 RLNQPGEQTAT 219
            +N P E   T
Sbjct: 203 -MNTPEEMAGT 193

BLAST of Cp4.1LG10g03250.1 vs. TAIR 10
Match: AT3G10820.2 (Transcription elongation factor (TFIIS) family protein )

HSP 1 Score: 70.5 bits (171), Expect = 3.2e-12
Identity = 96/382 (25.13%), Postives = 163/382 (42.67%), Query Frame = 0

Query: 34  DVWTFIDTAMEVASLDYGNQLKNRRDGIVERLYALTSPPTRCRNCDTDRYNDGRSNGCEI 93
           D++  ID A+ VA+ D+  + K+R D I E L++       C+          R  GC+ 
Sbjct: 23  DIFGIIDHAIMVAAADWPKEFKSRSDRIAELLFS-------CK--------VSRCIGCDH 82

Query: 94  KQSPVEAKEASPSTPQFVVVEGDDEGVDPYGGLFDDEQ-----KKVLEIKEQLEIPHQPE 153
            +  +   EA+    + V V G  +  D      + E+      +V+ I++ L      +
Sbjct: 83  LELSIAGDEAA---VEIVGVGGGGDRGDSGVATGEGEEASVSVDEVMRIRDILSNKDDEK 142

Query: 154 DA-LVELLQNLADMDITFQALKETDIGRHVNRLRKHSSNDVRRLVKHLVRKWKEIVDEWV 213
           D+ L+E L+ L  M ++   LK+T+IG+ VN LR+HSS+ + +L K L  +WK +VD+W 
Sbjct: 143 DSVLLESLRKLESMSMSVDILKDTEIGKAVNGLRRHSSDKISKLAKTLFAEWKRLVDQW- 202

Query: 214 RLNQPGEQTAT---------LIADSDSPQQKVPQNGYHQVPDFAYSPNPHN--------G 273
            +N P E   T          + D +      P    H +  +A  PN            
Sbjct: 203 -MNTPEEMAGTEGTPESLNLSVIDEEEAFPSPP----HDLDIYAPEPNGFELSQILDCLD 262

Query: 274 GSGSDRNNVEPE------------PKG-------------KSVPRRDAPPKPAQPAPMSS 333
             G+ R++VE +            PKG             +   R +A  +P + +    
Sbjct: 263 CDGNPRHSVESKHERKSQSSAGRRPKGTNDANVVGRYCNDQQTRREEADVRPMKHSATDV 322

Query: 334 YTPQNRQKEQQKE-----------ANFDSQKLAS-----------------ARKRLQENY 340
             P+ + K+ +++                +KLA                  A+++LQE+Y
Sbjct: 323 VEPKRQTKQSREQMVSAIQRKPTAVTEQKRKLAGPQQDKLKALDPDSKFEFAKRKLQESY 378

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4KFC73.3e-9156.67Probable mediator of RNA polymerase II transcription subunit 26c OS=Arabidopsis ... [more]
Q9FHK92.1e-1626.86Probable mediator of RNA polymerase II transcription subunit 26b OS=Arabidopsis ... [more]
F4J4Y54.5e-1125.13Probable mediator of RNA polymerase II transcription subunit 26a OS=Arabidopsis ... [more]
Q96MN53.7e-0538.24Transcription elongation factor A N-terminal and central domain-containing prote... [more]
B0UYI11.4e-0436.23Transcription elongation factor A N-terminal and central domain-containing prote... [more]
Match NameE-valueIdentityDescription
XP_023544969.15.82e-243100.00probable mediator of RNA polymerase II transcription subunit 26c [Cucurbita pepo... [more]
XP_022950583.17.92e-24199.11probable mediator of RNA polymerase II transcription subunit 26c [Cucurbita mosc... [more]
KAG6604266.11.60e-24099.11putative mediator of RNA polymerase II transcription subunit 26c, partial [Cucur... [more]
XP_022977926.19.19e-23898.82probable mediator of RNA polymerase II transcription subunit 26c [Cucurbita maxi... [more]
XP_038882890.13.53e-22894.08probable mediator of RNA polymerase II transcription subunit 26c [Benincasa hisp... [more]
Match NameE-valueIdentityDescription
A0A6J1GFA33.84e-24199.11probable mediator of RNA polymerase II transcription subunit 26c OS=Cucurbita mo... [more]
A0A6J1ILB44.45e-23898.82probable mediator of RNA polymerase II transcription subunit 26c OS=Cucurbita ma... [more]
A0A5A7SIU99.44e-22693.49Putative mediator of RNA polymerase II transcription subunit 26c OS=Cucumis melo... [more]
A0A1S3B2G29.44e-22693.49probable mediator of RNA polymerase II transcription subunit 26c OS=Cucumis melo... [more]
A0A0A0KHV92.59e-22392.90TFIIS N-terminal domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G4... [more]
Match NameE-valueIdentityDescription
AT5G09850.12.4e-9256.67Transcription elongation factor (TFIIS) family protein [more]
AT5G05140.11.5e-1726.86Transcription elongation factor (TFIIS) family protein [more]
AT3G10820.12.9e-1329.84Transcription elongation factor (TFIIS) family protein [more]
AT3G10820.23.2e-1225.13Transcription elongation factor (TFIIS) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 303..330
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 220..234
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 295..334
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 220..360
NoneNo IPR availablePANTHERPTHR47210:SF1MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 26C-RELATEDcoord: 22..360
NoneNo IPR availableCDDcd00183TFIIS_Icoord: 131..204
e-value: 1.96353E-19
score: 79.6598
IPR003617Transcription elongation factor, TFIIS/CRSP70, N-terminal, sub-typeSMARTSM00509TFS2_5coord: 134..205
e-value: 1.7E-9
score: 47.6
IPR017923Transcription factor IIS, N-terminalPFAMPF08711Med26coord: 154..203
e-value: 7.6E-13
score: 48.3
IPR017923Transcription factor IIS, N-terminalPROSITEPS51319TFIIS_Ncoord: 129..206
score: 25.527599
IPR035441TFIIS/LEDGF domain superfamilyGENE3D1.20.930.10Conserved domain common to transcription factors TFIIS, elongin A, CRSP70coord: 128..217
e-value: 1.8E-23
score: 84.1
IPR035441TFIIS/LEDGF domain superfamilySUPERFAMILY47676Conserved domain common to transcription factors TFIIS, elongin A, CRSP70coord: 132..213
IPR044790Mediator of RNA polymerase II transcription subunit 26c-likePANTHERPTHR47210MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 26C-RELATEDcoord: 22..360

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cp4.1LG10g03250Cp4.1LG10g03250gene


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG10g03250.1:three_prime_utr:001Cp4.1LG10g03250.1:three_prime_utr:001three_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG10g03250.1:exon:011Cp4.1LG10g03250.1:exon:011exon
Cp4.1LG10g03250.1:exon:010Cp4.1LG10g03250.1:exon:010exon
Cp4.1LG10g03250.1:exon:009Cp4.1LG10g03250.1:exon:009exon
Cp4.1LG10g03250.1:exon:008Cp4.1LG10g03250.1:exon:008exon
Cp4.1LG10g03250.1:exon:007Cp4.1LG10g03250.1:exon:007exon
Cp4.1LG10g03250.1:exon:006Cp4.1LG10g03250.1:exon:006exon
Cp4.1LG10g03250.1:exon:005Cp4.1LG10g03250.1:exon:005exon
Cp4.1LG10g03250.1:exon:004Cp4.1LG10g03250.1:exon:004exon
Cp4.1LG10g03250.1:exon:003Cp4.1LG10g03250.1:exon:003exon
Cp4.1LG10g03250.1:exon:002Cp4.1LG10g03250.1:exon:002exon
Cp4.1LG10g03250.1:exon:001Cp4.1LG10g03250.1:exon:001exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG10g03250.1:cds:011Cp4.1LG10g03250.1:cds:011CDS
Cp4.1LG10g03250.1:cds:010Cp4.1LG10g03250.1:cds:010CDS
Cp4.1LG10g03250.1:cds:009Cp4.1LG10g03250.1:cds:009CDS
Cp4.1LG10g03250.1:cds:008Cp4.1LG10g03250.1:cds:008CDS
Cp4.1LG10g03250.1:cds:007Cp4.1LG10g03250.1:cds:007CDS
Cp4.1LG10g03250.1:cds:006Cp4.1LG10g03250.1:cds:006CDS
Cp4.1LG10g03250.1:cds:005Cp4.1LG10g03250.1:cds:005CDS
Cp4.1LG10g03250.1:cds:004Cp4.1LG10g03250.1:cds:004CDS
Cp4.1LG10g03250.1:cds:003Cp4.1LG10g03250.1:cds:003CDS
Cp4.1LG10g03250.1:cds:002Cp4.1LG10g03250.1:cds:002CDS
Cp4.1LG10g03250.1:cds:001Cp4.1LG10g03250.1:cds:001CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG10g03250.1:five_prime_utr:001Cp4.1LG10g03250.1:five_prime_utr:001five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cp4.1LG10g03250.1Cp4.1LG10g03250.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005634 nucleus