Cp4.1LG09g01300.1 (mRNA) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG09g01300.1
TypemRNA
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptiongibberellin 20 oxidase 2-like
LocationCp4.1LG09: 720464 .. 726815 (-)
Sequence length2097
RNA-Seq ExpressionCp4.1LG09g01300.1
SyntenyCp4.1LG09g01300.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GCAACAATGGCGCTGAATTGCATGAACGCAACCGAGTCCTCACCGGCGTTGAACCATGTGAAGAATAGGGAGTATCGTCCGGCATTTTGTGGCTCCGACGAGACAAAGATACCGGAGGATTTCATTTGGCCAGAAGAGTTTAAGGCGGCGGAGGTGGTGCCGGAGCTGCATGTTCCACATATTGACTTGAAGAAGTTTTTTAGTGGCAATGAAAGTGACGTTGAAGAGGCGACGAGGCTGGTGGATGAGGCTTGTAGAAAACATGGATTCTTTGTGGTGGTGAACCATGGAGTTGATATGGAATTGATGAAAGGTGTTCATGAGTGTATTGATGAGTTCTTTACAATGCCTTTGGATGTGAAGCAGAGGGCTCAAAGGAAGGTAGGTGAGAATTATGGATATACTAATAGCTTCATTGGGAGATTCGCGTCCAAGCTTCCTTGGAAGGAAACCTTTTCCCTTCGTTGTGTGGCTGCTCAAAACTCCTCCGCTGCTCATGACTATGTTCTTGACACTTTAGGCTCGCCATTCTCCCATCATGGGTATCTAAATTCCACTATCTCTACCTATGCCTATTTAATCCTTACACTTTTTTTAATTGTTTGATGGTGTTTTAGGAAGTTGTATCAAGAGTGTGGGATAGCATTGAACGAGCTTGGTATGAAGATTGTGGAGCTTTTGGGGCTGAGCCTTGGCATTTCAAGAGAATGCTTCAAGAATTTCTATGAGGACAACGATTCAATATTGAGGCTTAATTATTACCCAACATGCGATAAGCCAGAATTGGTGTTGGGAACTGGCCCTCATAGTGATCCTACCTCCATCACAATCCTTCACCAAGACCATGTTAGTGGCCTTCAAGTGTTCGTAGATGATCAATGGTATTCAATTCCTCCAACCCCAGATTCCTTTGTCATCAACATTGGTGACACTTTCATGGTATGTATATGCAACACAAAATTAGAATTAGTCTTCCGGTCTCCACCTCCACGTAGTATGATATTGTCCACTTTGAATATAAACTCTCATGACTTTGCTTTGAGCTTCCTAAAAAGCTTCATGCCAATAGAGATATATTCTAATTCCTTACTTATAGACTCATGATGTTTCCTTAAATTAGCTAATGTGAGAGTTCCTGCCAATAATTCTCAACATTAACATTAGGGTTTTCATTTTTCATATGAATTGACTAGTTTTGAAATGGGTTGTTTTATTTTTTGTGTTTGAAGTCTCTGACAAATGGGATTTACAAGAGCTGCTTGCACCGAGCTGTAGTGAACTGCAAGGAATCAAGAAAATCATTGGCGTTCTTTCTATGTCCAGCGCATGACAAAGTGGTGAGAGCACCGGAGGAGTTGGTTGAGAAGAATCCACCAAGGAATTATTCAGATTACACATGGGAGATGTTGCTTAAAATGACCCAAAAGGATTACAGGGCTGATTTCAAGACTTTGGAAGCCTTCATGGCTCGTGTTCAAAAGGAAAAGTCGTTGGAGGACACTACCTCCCCAGCTGGTACGTCTACTTAAACCAATCTTAACTATCTCTCTATCTATGCCAACCAGATGTTCATTCATGAGCTTTGTTGGAAAGAAGAAGAAGACCACTTCGTGTTCGTTTCGTGTTTAGTCAATGTACACAATACACATGTTTATGCTTCACTCATCTTTTGTTCTGTTCGTAGGTTGTCATATAAATGTTTAAAAGCTTTCAATTTCTAAATCATTATCGATCTCTTTAAATTTATATGTTCTTCAAATATCTAATATTCTTGATCAACCATTATATCTAAAATGAATGGTAAAGATGGATAGAGTTCGTATCTAATAAACTCAACGTAAACCCAGGCATATTTTTAAATATTTTTTAATTGTCAAGCTATTTTTGCATCTTTTGAAGTAAACAAAAGGCCCACATGAAATTTTTAATTTGGTATTTAAATAGATTCTTAATATATTTGATATTTTTTTAATTTATAAACTTATTTGTAAAAAATTCACATAGCTAATTAAGAAAAAAATTATGGATATATAAATAAAGGAAATGATAGTTATCGGGAGACATTTTTAGAAAATAAAAAACAAAATTCATTGTTCCAACCATATCTATATATTCCATTACATAATAAAGAGAAAACTAGATCATTAGAGGTTCGTTGAAGACAAATCTCATGCAGAGGTTAGGTTAGCTTAGCTGGTGAACTTTACCTACACCATCATCTAGTCACCCAAGTTGGGGGAGCGACTCTTCTATGCTTGCCGGTTACTAAGACTACCTACATAATCCTATGTCTCCTTTCGGGTACATTTGTTGGGTAGGTAATTCAACTTAAATGTTGATCACACAACCCTAACCAGCCTAAGCTTGGTTTGACGGTATTGGTATCGAGTGAAAAAGAAAATGGAAGCTCTGTTGACATCGTACCAATATGGCTTGGAGGTTATCTTTGTCCGCTTACTTTGCTTATGCATTGAATGAACGGTGAATTGGGTTGAGGCGCGACCTTGTACACTATAGAAACAGTATCGTGTTTGCTAGTTTGAATATCATCCATGGAAGGTTAGTTGGTAGACCTTTCTACAGGACCATAAAACTCACCAGAGCTCGTGGGGAGCGACCTCCCATGTGCCGGTCAGCTACTGCGTCAACCTATGTGGTCCCACACCCCCCCTCCTCTCAAGAACGAGTTTATATCATAGAGGCCTTGCTCAGGAGACCGCTGGCCATTTCTACATGGTTGGGCACCTAGTCCTCTCGACGCACCTCCCCTATCCAGTTATGCCTACCTCAATGGTACAAGAATAAGAGTAGAATCTCGACGAAATGTGTATCGGAGTGGATAGGTAGCGAAGACTTTGATGATACCCTCACGCTGCTAACAACCTGAAGAACTTTTGTGGTCAAACTTAGATATCGTGGAGGATTACTAAATTCTTAGGAGTCTAGGCAGTTTAAATCTCATCTCTCGTTTCCAGGACACTTGGGGTTAGCTCCTCGAGCCTAATCCAAATATGTAGGTATACTCAGGTTTATTGAAAATATTTTGACCTAGGTCAATGTGTAAATTGCACTAGTTGTAGCGCCTCAATCTTAACTGAGTTTTCTCATGCATGCTTTACTAGAGAGATATACCTATCCATCACTTAAGCACGTAATCCATAATTTAAACTTCGCCTTTGTCGTATCTCGGAACTGAGAAGATCTATTGGATTCCTAGGGTGACTAGGTGGTTTTAAACTTCTTAGCCTGAGAGCACTAGAGTCTAGACCTTTTGACAGTTGCTCACGATCTTACTGTGCTCAGTCTATTAGAAGCATAATGATCTTGATCATTGTCTAACCAACCTAACTCTAACCTTATCTTTTAAAAATTTTTTTTTTTTTTTTTTTTTTTTCACTTCATTGTCTGCTTCTTTATTCTACAACACCATTTGTAAAATTCATTTTAAACAACACTTCAGCTTAAAGATAATAGCTAGACACATGAAGGCACTCGTGGACGATGATGGAGAACTTAGCTGAAAGAAGGGAAAAGAACATAAAAAAAAACATCTAAAAGTAAAACAAAAAACTTGAAGAACAAAACGACAAAGGACGAAAGAATGTCCACACTAAACAAGTGAGAGCATTCAAGAATATGGAGACACATTGAAAAATGTTAAGCATGCAGATATGGACACCGGGAGAGATGAAAATTAAATGTTCATTTTAAGGTACATGTCCGAGTGACAAAACTTTTAGTCACATCTCGAATTTTCATGAATTTATAAATAAAAAATACAATTTTAATGGTATGAGGCCTTTCGGAGAAGGTCAAAGCAAAGGTATGAGAGTTTATGTTCAGAGTCGCTATTCCTAACAAATTTTGTCACCCTATAAATGTCCCGAAATCCCCGGATCAATTCCATTCCGTTTGTTGGCTATCATACTCTCTTACTTATTCTATCTTCTCAAATCTCTCTCAAAAAGGAAAAAAAAAAAAACTATTTTTTTCTTTGCATGCAATAGCTTTCAATTGCAAGATGGCAACCGAATCCTCATTATCCGCTCACTCAAACTTGAACCAGGAGATGAAAAGGGAACGCCGTGAGACATTTGGGGGCTCCGAAGAGTCAAAGGTGCCGGAATATTTCATTTGGCCGGACGAGTTTAAGGGATCGCAGTTGGTGCCGGAGCTACATGTTCCACATATTGACGTGAATAAGTTTTTGAGTGGCAATGAAAGTGATGTAGAAGAGGCAACGAGGCTGGTGGATGAGGCTTGTAGAGAACATGGGTTCTTTGTAGTGGTGAACCATGGAGTTGATATGGAATTGATGAAAGGTGTTCATGAGTGTATGAATGAGTTCTTTACAATGCCTTTGGATGTGAAGCAGAGGGCTAAGAGGAAGGTAGGTGAGAGTTATGGATATACTAATAGCTTCTTTGGGAGATTCGCATCCAAGCTTCCATGGAAGGAAACCTTTTCCCTTCGCTGTGTGGCTGCTCAAAACTCATCCACTGCTCGTGACTATGTTATTGACATTTTAGGCTCAACATTCTCCCATCATGGGTATCTAAATTCCACTATCTAGTTTGATAAGATTAAGAGATATGTCTATTTAATTCATATAGTTTGAAGTTTCTAAATTTTTCATTTTTTCTAATCCATATTTACTAATAATTGGTTTTGACCTATTTTTTTTTAAGAAGGGTGAGGTGGGTTGATTTTTTAATATAGTTTTTCAAAACTTAAAATTTACACTAGGATATGATAATATGATATCTCCACAATAATATGATATTGTCCACTTTGAACATAAGTGGACAAGACTTCCTCCTAACAATCCTCCCCTTAGGCCTAATGGAGCCCTTGAACAACCTCCTATTAATCGAGACTCGAATCCACCCCTAAACAAAATACATTCTTTGTTCGACACTTTAATAACTTTTGACTGTACTTTTGAGGCTCACAACTTCTTTGTTCGACATTTGAGGATTCTATTGACATGACTAAGTTAAGGGCATTATGTGAGGAGCTACGAATCTCCACAATGGTATGTTATGAATCACGTCATGGCTTTGCTTTGGAGTTCCCCAAAAGGCCTCATATTAATGGAGATAGTATTGCTCACTTATAACCCCATGATCTTCCACTAAATTAACTAATGTGGGACTCAATCCCAATAATACTCAACAATCCTCCCCTTAATCGAGGCGACTCTTTTCTCTGGAGCCCTAGAACAAAGTACACCATTTGTTTGACACTTCAGTCACTTTTGACTACACTTTCGAGGCTCACACTTCTTTGTTTGATATTTGAGGATTCTATTGACATGACTAAGTTTAGGGCATGACTCTGATACCCTGTTATGAATCATGACTCTCCACAAAGGTATGATATTGTTTACTTTGAGCATAAGCTCTCATAGGTTTGTTTTGGACTTCCTCAAAAGGCCTCGAGATAGTATTCCTCTCTTATAAACCAATGATATTCCACTAAATTAACCAATGTGGGACTCACTCCCAATAATCCTCAACATAGTATGACATTTTCTACTTTGAGTATAAGCTCTCATGGTTTTGCTTTGGCCTTCTCAAGACGTCTCGTACCAATAGAGATCTATTCCTTACTTATAAACCGATGATCATTCCGAAAATTAGCCAATATGAAACTCACTCCCAATAATTCTCAACAGAAAAGATACTAGATACTAGTTGGACTTAAAATTTAATTCAATTAAAGGTGGGAAGTGAGGTTTGAATAGGTTTGATGGTGTTATAGGAAGGTATATCAGGAGTGTGGGAAAGCATTGAACGATCTGGGCATGAAGATATTGGAGATTTTGGGGCTGAGCCTTGGCGTTTCAAGAGAATACTTCAAGAATTTCTATGAGGACAACGATTCAATATTGAGGCTTAATTATTACCCAACATGCGACAAGCCAGAGGTTGTGTTGGGAACTGGCCCTCACACTGATCCCACTTCCGTCACAATCCTTCACCAAGACCATGTTAGTGGCCTTCAAGTGTGCGTCAATGATCAATGGTATTCAATTCCTCCGACCCCAGATTCCTTTGTCGTCAACATCGGTGACACTTTCACGGTAAGTCACACAAAATTACAATTAGGGTTCTTTATTTTTCGTCTTCCTTCTTCTAAACTCAACTAATTTTGAAATGGGTTGTTTGTTTTTTGTGTATTGAAGTCTCTCACAAATGGGATTTACAAGAGCTGCATACACCGAGCTGTAGTGAATTGCAAGGAAGCCAGAAAATCAATGGCCTTCTCTTTATGTCCAGCGCATGACAAAGTGGTGAGAGCACCGGAGGAATTGGTGGAGAAGAACCCACCACGAAAGTATCCAGATTAA

mRNA sequence

GCAACAATGGCGCTGAATTGCATGAACGCAACCGAGTCCTCACCGGCGTTGAACCATGTGAAGAATAGGGAGTATCGTCCGGCATTTTGTGGCTCCGACGAGACAAAGATACCGGAGGATTTCATTTGGCCAGAAGAGTTTAAGGCGGCGGAGGTGGTGCCGGAGCTGCATGTTCCACATATTGACTTGAAGAAGTTTTTTAGTGGCAATGAAAGTGACGTTGAAGAGGCGACGAGGCTGGTGGATGAGGCTTGTAGAAAACATGGATTCTTTGTGGTGGTGAACCATGGAGTTGATATGGAATTGATGAAAGGTGTTCATGAGTGTATTGATGAGTTCTTTACAATGCCTTTGGATGTGAAGCAGAGGGCTCAAAGGAAGGTAGGTGAGAATTATGGATATACTAATAGCTTCATTGGGAGATTCGCGTCCAAGCTTCCTTGGAAGGAAACCTTTTCCCTTCGTTGTGTGGCTGCTCAAAACTCCTCCGCTGCTCATGACTATGTTCTTGACACTTTAGGCTCGCCATTCTCCCATCATGGGAAGTTGTATCAAGAGTGTGGGATAGCATTGAACGAGCTTGGTATGAAGATTGTGGAGCTTTTGGGGCTGAGCCTTGGCATTTCAAGAGAATGCTTCAAGAATTTCTATGAGGACAACGATTCAATATTGAGGCTTAATTATTACCCAACATGCGATAAGCCAGAATTGGTGTTGGGAACTGGCCCTCATAGTGATCCTACCTCCATCACAATCCTTCACCAAGACCATGTTAGTGGCCTTCAAGTGTTCGTAGATGATCAATGGTATTCAATTCCTCCAACCCCAGATTCCTTTTCTCTGACAAATGGGATTTACAAGAGCTGCTTGCACCGAGCTGTAGTGAACTGCAAGGAATCAAGAAAATCATTGGCGTTCTTTCTATGTCCAGCGCATGACAAAGTGGTGAGAGCACCGGAGGAGTTGGTTGAGAAGAATCCACCAAGGAATTATTCAGATTACACATGGGAGATGTTGCTTAAAATGACCCAAAAGGATTACAGGGCTGATTTCAAGACTTTGGAAGCCTTCATGGCTCGTATGGCAACCGAATCCTCATTATCCGCTCACTCAAACTTGAACCAGGAGATGAAAAGGGAACGCCGTGAGACATTTGGGGGCTCCGAAGAGTCAAAGGTGCCGGAATATTTCATTTGGCCGGACGAGTTTAAGGGATCGCAGTTGGTGCCGGAGCTACATGTTCCACATATTGACGTGAATAAGTTTTTGAGTGGCAATGAAAGTGATGTAGAAGAGGCAACGAGGCTGGTGGATGAGGCTTGTAGAGAACATGGGTTCTTTGTAGTGGTGAACCATGGAGTTGATATGGAATTGATGAAAGGTGTTCATGAGTGTATGAATGAGTTCTTTACAATGCCTTTGGATGTGAAGCAGAGGGCTAAGAGGAAGGTAGGTGAGAGTTATGGATATACTAATAGCTTCTTTGGGAGATTCGCATCCAAGCTTCCATGGAAGGAAACCTTTTCCCTTCGCTGTGTGGCTGCTCAAAACTCATCCACTGCTCGTGACTATGTTATTGACATTTTAGGCTCAACATTCTCCCATCATGGGAAGGTATATCAGGAGTGTGGGAAAGCATTGAACGATCTGGGCATGAAGATATTGGAGATTTTGGGGCTGAGCCTTGGCGTTTCAAGAGAATACTTCAAGAATTTCTATGAGGACAACGATTCAATATTGAGGCTTAATTATTACCCAACATGCGACAAGCCAGAGGTTGTGTTGGGAACTGGCCCTCACACTGATCCCACTTCCGTCACAATCCTTCACCAAGACCATGTTAGTGGCCTTCAAGTGTGCGTCAATGATCAATGGTATTCAATTCCTCCGACCCCAGATTCCTTTGTCGTCAACATCGGTGACACTTTCACGTCTCTCACAAATGGGATTTACAAGAGCTGCATACACCGAGCTGTAGTGAATTGCAAGGAAGCCAGAAAATCAATGGCCTTCTCTTTATGTCCAGCGCATGACAAAGTGGTGAGAGCACCGGAGGAATTGGTGGAGAAGAACCCACCACGAAAGTATCCAGATTAA

Coding sequence (CDS)

GCAACAATGGCGCTGAATTGCATGAACGCAACCGAGTCCTCACCGGCGTTGAACCATGTGAAGAATAGGGAGTATCGTCCGGCATTTTGTGGCTCCGACGAGACAAAGATACCGGAGGATTTCATTTGGCCAGAAGAGTTTAAGGCGGCGGAGGTGGTGCCGGAGCTGCATGTTCCACATATTGACTTGAAGAAGTTTTTTAGTGGCAATGAAAGTGACGTTGAAGAGGCGACGAGGCTGGTGGATGAGGCTTGTAGAAAACATGGATTCTTTGTGGTGGTGAACCATGGAGTTGATATGGAATTGATGAAAGGTGTTCATGAGTGTATTGATGAGTTCTTTACAATGCCTTTGGATGTGAAGCAGAGGGCTCAAAGGAAGGTAGGTGAGAATTATGGATATACTAATAGCTTCATTGGGAGATTCGCGTCCAAGCTTCCTTGGAAGGAAACCTTTTCCCTTCGTTGTGTGGCTGCTCAAAACTCCTCCGCTGCTCATGACTATGTTCTTGACACTTTAGGCTCGCCATTCTCCCATCATGGGAAGTTGTATCAAGAGTGTGGGATAGCATTGAACGAGCTTGGTATGAAGATTGTGGAGCTTTTGGGGCTGAGCCTTGGCATTTCAAGAGAATGCTTCAAGAATTTCTATGAGGACAACGATTCAATATTGAGGCTTAATTATTACCCAACATGCGATAAGCCAGAATTGGTGTTGGGAACTGGCCCTCATAGTGATCCTACCTCCATCACAATCCTTCACCAAGACCATGTTAGTGGCCTTCAAGTGTTCGTAGATGATCAATGGTATTCAATTCCTCCAACCCCAGATTCCTTTTCTCTGACAAATGGGATTTACAAGAGCTGCTTGCACCGAGCTGTAGTGAACTGCAAGGAATCAAGAAAATCATTGGCGTTCTTTCTATGTCCAGCGCATGACAAAGTGGTGAGAGCACCGGAGGAGTTGGTTGAGAAGAATCCACCAAGGAATTATTCAGATTACACATGGGAGATGTTGCTTAAAATGACCCAAAAGGATTACAGGGCTGATTTCAAGACTTTGGAAGCCTTCATGGCTCGTATGGCAACCGAATCCTCATTATCCGCTCACTCAAACTTGAACCAGGAGATGAAAAGGGAACGCCGTGAGACATTTGGGGGCTCCGAAGAGTCAAAGGTGCCGGAATATTTCATTTGGCCGGACGAGTTTAAGGGATCGCAGTTGGTGCCGGAGCTACATGTTCCACATATTGACGTGAATAAGTTTTTGAGTGGCAATGAAAGTGATGTAGAAGAGGCAACGAGGCTGGTGGATGAGGCTTGTAGAGAACATGGGTTCTTTGTAGTGGTGAACCATGGAGTTGATATGGAATTGATGAAAGGTGTTCATGAGTGTATGAATGAGTTCTTTACAATGCCTTTGGATGTGAAGCAGAGGGCTAAGAGGAAGGTAGGTGAGAGTTATGGATATACTAATAGCTTCTTTGGGAGATTCGCATCCAAGCTTCCATGGAAGGAAACCTTTTCCCTTCGCTGTGTGGCTGCTCAAAACTCATCCACTGCTCGTGACTATGTTATTGACATTTTAGGCTCAACATTCTCCCATCATGGGAAGGTATATCAGGAGTGTGGGAAAGCATTGAACGATCTGGGCATGAAGATATTGGAGATTTTGGGGCTGAGCCTTGGCGTTTCAAGAGAATACTTCAAGAATTTCTATGAGGACAACGATTCAATATTGAGGCTTAATTATTACCCAACATGCGACAAGCCAGAGGTTGTGTTGGGAACTGGCCCTCACACTGATCCCACTTCCGTCACAATCCTTCACCAAGACCATGTTAGTGGCCTTCAAGTGTGCGTCAATGATCAATGGTATTCAATTCCTCCGACCCCAGATTCCTTTGTCGTCAACATCGGTGACACTTTCACGTCTCTCACAAATGGGATTTACAAGAGCTGCATACACCGAGCTGTAGTGAATTGCAAGGAAGCCAGAAAATCAATGGCCTTCTCTTTATGTCCAGCGCATGACAAAGTGGTGAGAGCACCGGAGGAATTGGTGGAGAAGAACCCACCACGAAAGTATCCAGATTAA

Protein sequence

ATMALNCMNATESSPALNHVKNREYRPAFCGSDETKIPEDFIWPEEFKAAEVVPELHVPHIDLKKFFSGNESDVEEATRLVDEACRKHGFFVVVNHGVDMELMKGVHECIDEFFTMPLDVKQRAQRKVGENYGYTNSFIGRFASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGSPFSHHGKLYQECGIALNELGMKIVELLGLSLGISRECFKNFYEDNDSILRLNYYPTCDKPELVLGTGPHSDPTSITILHQDHVSGLQVFVDDQWYSIPPTPDSFSLTNGIYKSCLHRAVVNCKESRKSLAFFLCPAHDKVVRAPEELVEKNPPRNYSDYTWEMLLKMTQKDYRADFKTLEAFMARMATESSLSAHSNLNQEMKRERRETFGGSEESKVPEYFIWPDEFKGSQLVPELHVPHIDVNKFLSGNESDVEEATRLVDEACREHGFFVVVNHGVDMELMKGVHECMNEFFTMPLDVKQRAKRKVGESYGYTNSFFGRFASKLPWKETFSLRCVAAQNSSTARDYVIDILGSTFSHHGKVYQECGKALNDLGMKILEILGLSLGVSREYFKNFYEDNDSILRLNYYPTCDKPEVVLGTGPHTDPTSVTILHQDHVSGLQVCVNDQWYSIPPTPDSFVVNIGDTFTSLTNGIYKSCIHRAVVNCKEARKSMAFSLCPAHDKVVRAPEELVEKNPPRKYPD
Homology
BLAST of Cp4.1LG09g01300.1 vs. ExPASy Swiss-Prot
Match: Q39110 (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 382.1 bits (980), Expect = 1.3e-104
Identity = 186/334 (55.69%), Postives = 230/334 (68.86%), Query Frame = 0

Query: 34  ETKIPEDFIWPEEFKAAEVVPELHVPHIDLKKFFSGNESDVEEATRLVDEACRKHGFFVV 93
           +  IP  FIWP++ K +  V EL VP IDL+   S + S   +A+RL+ EAC+KHGFF+V
Sbjct: 37  QANIPNQFIWPDDEKPSINVLELDVPLIDLQNLLS-DPSSTLDASRLISEACKKHGFFLV 96

Query: 94  VNHGVDMELMKGVHECIDEFFTMPLDVKQRAQRKVGENYGYTNSFIGRFASKLPWKETFS 153
           VNHG+  EL+   HE    FF MPL  KQR  RK GE+ GY +SF GRF++KLPWKET S
Sbjct: 97  VNHGISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLS 156

Query: 154 LR-CVAAQNSSAAHDYVLDTLGSPFSHHGKLYQECGIALNELGMKIVELLGLSLGISREC 213
            R C     S +  DY  D LG  F   GK+YQE   A++ L +KI+ELLGLSLG+ R+ 
Sbjct: 157 FRFCDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDY 216

Query: 214 FKNFYEDNDSILRLNYYPTCDKPELVLGTGPHSDPTSITILHQDHVSGLQVFVDDQWYSI 273
           F+ F+E+NDSI+RLNYYP C KP+L LGTGPH DPTS+TILHQDHV+GLQVFV++QW SI
Sbjct: 217 FREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSI 276

Query: 274 PPTPDSF---------SLTNGIYKSCLHRAVVNCKESRKSLAFFLCPAHDKVVRAPEELV 333
            P P +F         +L+N  YKSCLHRAVVN +  RKSLAFFLCP  D+VV  P EL+
Sbjct: 277 RPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRELL 336

Query: 334 EKNPPRNYSDYTWEMLLKMTQKDYRADFKTLEAF 358
           +    R Y D+TW M L+ TQK YRAD  TL+AF
Sbjct: 337 DSITSRRYPDFTWSMFLEFTQKHYRADMNTLQAF 369

BLAST of Cp4.1LG09g01300.1 vs. ExPASy Swiss-Prot
Match: Q39111 (Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 5.1e-104
Identity = 182/343 (53.06%), Postives = 241/343 (70.26%), Query Frame = 0

Query: 34  ETKIPEDFIWPEEFKAAEVVPELHVPHIDLKKFFSGNESDVEEATRLVDEACRKHGFFVV 93
           +++IP  FIWP+E K +  +PEL+VP IDL      ++    EA R++ EAC KHGFF+V
Sbjct: 39  QSQIPNQFIWPDEEKPSIDIPELNVPFIDL-----SSQDSTLEAPRVIAEACTKHGFFLV 98

Query: 94  VNHGVDMELMKGVHECIDEFFTMPLDVKQRAQRKVGENYGYTNSFIGRFASKLPWKETFS 153
           VNHGV   L+   H  ++ FF MPL  KQ+AQRK GE+ GY +SF GRF++KLPWKET S
Sbjct: 99  VNHGVSESLIADAHRLMESFFDMPLAGKQKAQRKPGESCGYASSFTGRFSTKLPWKETLS 158

Query: 154 LRCVAAQNSS-AAHDYVLDTLGSPFSHHGKLYQECGIALNELGMKIVELLGLSLGISREC 213
            +     + S    DY  DTLG  F   GK+YQ+   A++ L +KI+ELLGLSLG++R+ 
Sbjct: 159 FQFSNDNSGSRTVQDYFSDTLGQEFEQFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRDY 218

Query: 214 FKNFYEDNDSILRLNYYPTCDKPELVLGTGPHSDPTSITILHQDHVSGLQVFVDDQWYSI 273
           F+ F+E+NDSI+RLN+YP C  P+L LGTGPH DP+S+TILHQDHV+GLQVFVD+QW SI
Sbjct: 219 FRGFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQDHVNGLQVFVDNQWQSI 278

Query: 274 PPTPDSF---------SLTNGIYKSCLHRAVVNCKESRKSLAFFLCPAHDKVVRAPEELV 333
            P P +F         +L+NGI+KSCLHRAVVN + +RKS+AFFLCP  DKVV+ P +++
Sbjct: 279 RPNPKAFVVNIGDTFMALSNGIFKSCLHRAVVNRESARKSMAFFLCPKKDKVVKPPSDIL 338

Query: 334 EKNPPRNYSDYTWEMLLKMTQKDYRADFKTLEAFMARMATESS 367
           EK   R Y D+TW M L+ TQK YRAD  TL++F   + T ++
Sbjct: 339 EKMKTRKYPDFTWSMFLEFTQKHYRADVNTLDSFSNWVITNNN 376

BLAST of Cp4.1LG09g01300.1 vs. ExPASy Swiss-Prot
Match: Q39112 (Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=GA20OX3 PE=2 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 1.1e-101
Identity = 182/334 (54.49%), Postives = 226/334 (67.66%), Query Frame = 0

Query: 37  IPEDFIWPEEFKAAEVVPELHVPHIDLKKFFSGNESDVEEATRLVDEACRKHGFFVVVNH 96
           IP+ F+WP+  K +  V  L VP IDL  F SG+     EATRLV +A  KHGFF++ NH
Sbjct: 37  IPQQFVWPDHEKPSTDVQPLQVPLIDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNH 96

Query: 97  GVDMELMKGVHECIDEFFTMPLDVKQRAQRKVGENYGYTNSFIGRFASKLPWKETFSLRC 156
           GVD  L+   +  +D FF  P   KQ+AQRK GE+ GY +SF+GRF+SKLPWKET S + 
Sbjct: 97  GVDESLLSRAYLHMDSFFKAPACEKQKAQRKWGESSGYASSFVGRFSSKLPWKETLSFKF 156

Query: 157 VAAQ--NSSAAHDYVLDTLGSPFSHHGKLYQECGIALNELGMKIVELLGLSLGISRECFK 216
              +  +S    D+V   +G  +   GK+YQE   A+N L +KI+ELLG+SLG+ R  FK
Sbjct: 157 SPEEKIHSQTVKDFVSKKMGDGYEDFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFK 216

Query: 217 NFYEDNDSILRLNYYPTCDKPELVLGTGPHSDPTSITILHQDHVSGLQVFVDDQWYSIPP 276
            F+ED+DSI RLNYYP C +PEL LGTGPH DPTS+TILHQD V GLQVFVD++W SIPP
Sbjct: 217 EFFEDSDSIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPP 276

Query: 277 TPDSF---------SLTNGIYKSCLHRAVVNCKESRKSLAFFLCPAHDKVVRAPEELVE- 336
            P +F         +LTNG YKSCLHRAVVN +  RK+ AFFLCP  +KVV+ PEELV  
Sbjct: 277 NPHAFVVNIGDTFMALTNGRYKSCLHRAVVNSERERKTFAFFLCPKGEKVVKPPEELVNG 336

Query: 337 -KNPPRNYSDYTWEMLLKMTQKDYRADFKTLEAF 358
            K+  R Y D+TW M L+ TQK YRAD  TL+ F
Sbjct: 337 VKSGERKYPDFTWSMFLEFTQKHYRADMNTLDEF 370

BLAST of Cp4.1LG09g01300.1 vs. ExPASy Swiss-Prot
Match: O04707 (Gibberellin 20 oxidase 1-A OS=Triticum aestivum OX=4565 GN=GA20ox1A PE=2 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 8.4e-99
Identity = 182/345 (52.75%), Postives = 224/345 (64.93%), Query Frame = 0

Query: 26  RPAFCG---SDETKIPEDFIWPE-EFKAAEVVPELHVPHIDLKKFFSGNESDVEEATRLV 85
           RP F     S  + IP  FIWPE E    +   ELHVP I++    SG+ +   E TRLV
Sbjct: 3   RPVFDAAVLSGRSDIPSQFIWPEGESPTPDAAEELHVPLINIGGMLSGDAAAAAEVTRLV 62

Query: 86  DEACRKHGFFVVVNHGVDMELMKGVHECIDEFFTMPLDVKQRAQRKVGENYGYTNSFIGR 145
            EAC +HGFF VVNHG+D EL+   H C+D FFTMPL  KQRA R+ GE+ GY +SF GR
Sbjct: 63  GEACERHGFFQVVNHGIDAELLADAHRCVDNFFTMPLPEKQRALRRPGESCGYASSFTGR 122

Query: 146 FASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGSPFSHHGKLYQECGIALNELGMKIVEL 205
           FASKLPWKET S R     + +   DY++ TLG      G++Y      ++ L ++I+E+
Sbjct: 123 FASKLPWKETLSFRS-CPSDPALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEV 182

Query: 206 LGLSLGISRECFKNFYEDNDSILRLNYYPTCDKPELVLGTGPHSDPTSITILHQDHVSGL 265
           LG SLG+ R  ++ F+E NDSI+RLNYYP C +P   LGTGPH DPTS+TILHQD+V GL
Sbjct: 183 LGESLGVGRAHYRRFFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDNVGGL 242

Query: 266 QVFVDDQWYSIPPTPDSF---------SLTNGIYKSCLHRAVVNCKESRKSLAFFLCPAH 325
           QV  + +W SI P  D+F         +L+NG YKSCLHRAVVN +  RKSLAFFLCP  
Sbjct: 243 QVHTEGRWRSIRPRADAFVVNIGDTFMALSNGRYKSCLHRAVVNSRVPRKSLAFFLCPEM 302

Query: 326 DKVVRAPEELVEKNPPRNYSDYTWEMLLKMTQKDYRADFKTLEAF 358
           DKVV  P  LV+ + PR Y D+TW  LL  TQK YRAD KTLE F
Sbjct: 303 DKVVAPPGTLVDASNPRAYPDFTWRSLLDFTQKHYRADMKTLEVF 346

BLAST of Cp4.1LG09g01300.1 vs. ExPASy Swiss-Prot
Match: O04706 (Gibberellin 20 oxidase 1-B OS=Triticum aestivum OX=4565 GN=GA20ox1B PE=2 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 8.4e-99
Identity = 181/336 (53.87%), Postives = 218/336 (64.88%), Query Frame = 0

Query: 32  SDETKIPEDFIWPE-EFKAAEVVPELHVPHIDLKKFFSGNESDVEEATRLVDEACRKHGF 91
           S    IP  FIWPE E    +   ELHVP ID+    SG+     E TRLV EAC +HGF
Sbjct: 12  SGRADIPSQFIWPEGESPTPDAAEELHVPLIDIGGMLSGDPRATAEVTRLVGEACERHGF 71

Query: 92  FVVVNHGVDMELMKGVHECIDEFFTMPLDVKQRAQRKVGENYGYTNSFIGRFASKLPWKE 151
           F VVNHG+D EL+   H C+D FFTMPL  KQRA R+ GE+ GY +SF GRFASKLPWKE
Sbjct: 72  FQVVNHGIDAELLADAHRCVDAFFTMPLPEKQRALRRPGESCGYASSFTGRFASKLPWKE 131

Query: 152 TFSLRCVAAQNSSAAHDYVLDTLGSPFSHHGKLYQECGIALNELGMKIVELLGLSLGISR 211
           T S R     + +   DY++ TLG      G++Y      ++ L ++I+E+LG SLG+ R
Sbjct: 132 TLSFRS-CPSDPALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESLGVGR 191

Query: 212 ECFKNFYEDNDSILRLNYYPTCDKPELVLGTGPHSDPTSITILHQDHVSGLQVFVDDQWY 271
             ++ F+E NDSI+RLNYYP C +P   LGTGPH DPTS+TILHQD+V GLQV  + +W 
Sbjct: 192 AHYRRFFEGNDSIMRLNYYPPCQRPMETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRWR 251

Query: 272 SIPPTPDSF---------SLTNGIYKSCLHRAVVNCKESRKSLAFFLCPAHDKVVRAPEE 331
           SI P  D+F         +L+NG YKSCLHRAVVN K  RKSLAFFLCP  DKVV  P  
Sbjct: 252 SIRPRADAFVVNIGDTFMALSNGRYKSCLHRAVVNSKVPRKSLAFFLCPEMDKVVAPPGT 311

Query: 332 LVEKNPPRNYSDYTWEMLLKMTQKDYRADFKTLEAF 358
           LV+   PR Y D+TW  LL  TQK YRAD KTLE F
Sbjct: 312 LVDAANPRAYPDFTWRSLLDFTQKHYRADMKTLEVF 346

BLAST of Cp4.1LG09g01300.1 vs. NCBI nr
Match: XP_023542034.1 (uncharacterized protein LOC111802006 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1057 bits (2734), Expect = 0.0
Identity = 523/766 (68.28%), Postives = 596/766 (77.81%), Query Frame = 0

Query: 10  ATESSPALNHVKNRE----YRPAFCGSDETKIPEDFIWPEEFKAAEVVPELHVPHIDLKK 69
           ATE+  + N   N+E    YR  F G DE+K+PE F+WP++ K ++ VP LHVPHID+ K
Sbjct: 2   ATETLLSANSDVNQETKGEYRGTFAGPDESKVPESFVWPDDLKGSQEVPVLHVPHIDINK 61

Query: 70  FFSGNESDVEEATRLVDEACRKHGFFVVVNHGVDMELMKGVHECIDEFFTMPLDVKQRAQ 129
           F SG ESDVEE TRLVDEACRKHGFFVVVNHGVDM++M+ +HEC+ EFF+MP+DVKQRA 
Sbjct: 62  FMSGKESDVEEVTRLVDEACRKHGFFVVVNHGVDMKVMESLHECMSEFFSMPMDVKQRAH 121

Query: 130 RKVGENYGYTNSFIGRFASKLPWKETFSLRCVAA-QNSSAAHDYVLDTLGSPFSHHGKLY 189
           RK GEN+GY  SF  RF+S LPWKETFSL CVAA +NSS+A+DYV++ LGS FSHHGK+Y
Sbjct: 122 RKFGENFGYAYSFFVRFSSNLPWKETFSLPCVAAGENSSSAYDYVIEKLGSSFSHHGKVY 181

Query: 190 QECGIALNELGMKIVELLGLSLGISRECFKNFYEDNDSILRLNYYPTCDKPELVLGTGPH 249
           Q+C  A+N+LGMKI+E+LGLSLG+SRE F+NFYEDNDSI+RLNYYP C KPELVLGTGPH
Sbjct: 182 QDCANAVNDLGMKILEVLGLSLGVSREYFRNFYEDNDSIMRLNYYPPCAKPELVLGTGPH 241

Query: 250 SDPTSITILHQDHVSGLQVFVDDQWYSIPPTPDSF---------SLTNGIYKSCLHRAVV 309
            DPTSITILHQD VSG QV VDDQWYSIPPTPDSF         SLTNGIYKSC+HRAVV
Sbjct: 242 CDPTSITILHQDKVSGFQVSVDDQWYSIPPTPDSFVINIGDTFKSLTNGIYKSCVHRAVV 301

Query: 310 NCKESRKSLAFFLCPAHDKVVRAPEELVEKNPPRNYSDYTWEMLLKMTQKDYRADFKTLE 369
           N +  RK++AFF+CP HDKVVRAPEELVEKNPPR Y DYTW  LL++TQK YR D  TLE
Sbjct: 302 NSESVRKTMAFFICPNHDKVVRAPEELVEKNPPRKYPDYTWGTLLEITQKHYRPDCNTLE 361

Query: 370 AF---------------------------------------------------------- 429
           A                                                           
Sbjct: 362 ALTDWIQKGKPLDNGSTLTAPKSKEFSLMKPQIPNLPFLPPYKSPQLPPIQIHSVSSLIL 421

Query: 430 -----MARMATESSLSAHSNLNQEMKRERRETFGGSEESKVPEYFIWPDEFKGSQLVPEL 489
                MA     ++ +A SNLN+E K E R  FGGS+ESKVPE FIW ++F+ S+L+P L
Sbjct: 422 SSLHTMALNGKVATEAAPSNLNEETKGEYRPPFGGSDESKVPEDFIWSEKFEASELLPVL 481

Query: 490 HVPHIDVNKFLSGNESDVEEATRLVDEACREHGFFVVVNHGVDMELMKGVHECMNEFFTM 549
            VP+ID+ KF+SG++S VEEATRLVDEACR+HG F VVNHGVDME+M  VH+CMNEFFTM
Sbjct: 482 DVPNIDLEKFMSGDKSYVEEATRLVDEACRQHGIFFVVNHGVDMEMMGRVHDCMNEFFTM 541

Query: 550 PLDVKQRAKRKVGESYGYTNSFFGRFASKLPWKETFSLRCVAAQNSSTARDYVIDILGST 609
           PLDVKQRAKR+VGESYGYTNSFFGRFA+ LPWKETFSLRC+AAQNSS A DYV+D LGS+
Sbjct: 542 PLDVKQRAKRRVGESYGYTNSFFGRFANNLPWKETFSLRCLAAQNSSAAHDYVLDTLGSS 601

Query: 610 FSHHGKVYQECGKALNDLGMKILEILGLSLGVSREYFKNFYEDNDSILRLNYYPTCDKPE 669
           FSHHGK YQECG ALN LG KI+E+LGLSLGVSREYFKNFYEDNDSILRLNYYP CDKPE
Sbjct: 602 FSHHGKAYQECGIALNQLGTKIVELLGLSLGVSREYFKNFYEDNDSILRLNYYPPCDKPE 661

Query: 670 VVLGTGPHTDPTSVTILHQDHVSGLQVCVNDQWYSIPPTPDSFVVNIGDTFTSLTNGIYK 698
           VVLGTGPHTDPTSVT+LHQD VSGLQVC NDQWYSIPP P++FV+NIGDTFTSLTNGIYK
Sbjct: 662 VVLGTGPHTDPTSVTLLHQDPVSGLQVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYK 721

BLAST of Cp4.1LG09g01300.1 vs. NCBI nr
Match: XP_023541911.1 (gibberellin 20 oxidase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 743 bits (1917), Expect = 1.07e-264
Identity = 361/374 (96.52%), Postives = 362/374 (96.79%), Query Frame = 0

Query: 3   MALNCMNATESSPALNHVKNREYRPAFCGSDETKIPEDFIWPEEFKAAEVVPELHVPHID 62
           MALNCMNATESSPALNHVKNREYRPAFCGSDETKIPEDFIWPEEFKAAEVVPELHVPHID
Sbjct: 1   MALNCMNATESSPALNHVKNREYRPAFCGSDETKIPEDFIWPEEFKAAEVVPELHVPHID 60

Query: 63  LKKFFSGNESDVEEATRLVDEACRKHGFFVVVNHGVDMELMKGVHECIDEFFTMPLDVKQ 122
           LKKFFSGNESDVEEATRLVDEACRKHGFFVVVNHGVDMELMKGVHECIDEFFTMPLDVKQ
Sbjct: 61  LKKFFSGNESDVEEATRLVDEACRKHGFFVVVNHGVDMELMKGVHECIDEFFTMPLDVKQ 120

Query: 123 RAQRKVGENYGYTNSFIGRFASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGSPFSHHGK 182
           RAQRKVGENYGYTNSFIGRFASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGSPFSHHGK
Sbjct: 121 RAQRKVGENYGYTNSFIGRFASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGSPFSHHGK 180

Query: 183 LYQECGIALNELGMKIVELLGLSLGISRECFKNFYEDNDSILRLNYYPTCDKPELVLGTG 242
           LYQECGIALNELGMKIVELLGLSLGISRECFKNFYEDNDSILRLNYYPTCDKPELVLGTG
Sbjct: 181 LYQECGIALNELGMKIVELLGLSLGISRECFKNFYEDNDSILRLNYYPTCDKPELVLGTG 240

Query: 243 PHSDPTSITILHQDHVSGLQVFVDDQWYSIPPTPDSF---------SLTNGIYKSCLHRA 302
           PHSDPTSITILHQDHVSGLQVFVDDQWYSIPPTPDSF         SLTNGIYKSCLHRA
Sbjct: 241 PHSDPTSITILHQDHVSGLQVFVDDQWYSIPPTPDSFVINIGDTFMSLTNGIYKSCLHRA 300

Query: 303 VVNCKESRKSLAFFLCPAHDKVVRAPEELVEKNPPRNYSDYTWEMLLKMTQKDYRADFKT 362
           VVNCKESRKSLAFFLCPAHDKVVRAPEELVEKNPPRNYSDYTWEMLLKMTQKDYRADFKT
Sbjct: 301 VVNCKESRKSLAFFLCPAHDKVVRAPEELVEKNPPRNYSDYTWEMLLKMTQKDYRADFKT 360

Query: 363 LEAFMARMATESSL 367
           LEAFMAR+  E SL
Sbjct: 361 LEAFMARVQKEKSL 374

BLAST of Cp4.1LG09g01300.1 vs. NCBI nr
Match: XP_022945557.1 (gibberellin 20 oxidase 1-like [Cucurbita moschata])

HSP 1 Score: 730 bits (1884), Expect = 1.11e-259
Identity = 355/374 (94.92%), Postives = 359/374 (95.99%), Query Frame = 0

Query: 3   MALNCMNATESSPALNHVKNREYRPAFCGSDETKIPEDFIWPEEFKAAEVVPELHVPHID 62
           MALNCMNATESSPALN V+NREYRPAFCGSDE+KIPEDFIWPEEFKAAEVVPELHVPHID
Sbjct: 1   MALNCMNATESSPALNQVRNREYRPAFCGSDESKIPEDFIWPEEFKAAEVVPELHVPHID 60

Query: 63  LKKFFSGNESDVEEATRLVDEACRKHGFFVVVNHGVDMELMKGVHECIDEFFTMPLDVKQ 122
           LKKFFSGNESDVEEATRLVDEACRKHGFFVVVNHGVDMELMKGVHECIDEFFTMPLDVKQ
Sbjct: 61  LKKFFSGNESDVEEATRLVDEACRKHGFFVVVNHGVDMELMKGVHECIDEFFTMPLDVKQ 120

Query: 123 RAQRKVGENYGYTNSFIGRFASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGSPFSHHGK 182
           RAQRKVGENYGYTNSFIGRFASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGS FSHHGK
Sbjct: 121 RAQRKVGENYGYTNSFIGRFASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHGK 180

Query: 183 LYQECGIALNELGMKIVELLGLSLGISRECFKNFYEDNDSILRLNYYPTCDKPELVLGTG 242
           LYQECGIALNELGMKIVELLGLSLGISRECFKNFYEDNDSILRLNYYP C+KPELVLGTG
Sbjct: 181 LYQECGIALNELGMKIVELLGLSLGISRECFKNFYEDNDSILRLNYYPICEKPELVLGTG 240

Query: 243 PHSDPTSITILHQDHVSGLQVFVDDQWYSIPPTPDSF---------SLTNGIYKSCLHRA 302
           PHSDPTSITILHQDHVSGLQVFVDDQWYSIPPTPDSF         SLTNGIYKSCLHRA
Sbjct: 241 PHSDPTSITILHQDHVSGLQVFVDDQWYSIPPTPDSFVINIGDTFMSLTNGIYKSCLHRA 300

Query: 303 VVNCKESRKSLAFFLCPAHDKVVRAPEELVEKNPPRNYSDYTWEMLLKMTQKDYRADFKT 362
           VVNCKESRKSLAFFLCPAHDKVVRAPEELVEKNPPRNYSDYTWEMLLKMTQKDYRADFKT
Sbjct: 301 VVNCKESRKSLAFFLCPAHDKVVRAPEELVEKNPPRNYSDYTWEMLLKMTQKDYRADFKT 360

Query: 363 LEAFMARMATESSL 367
           LEAFMAR+  E SL
Sbjct: 361 LEAFMARVQKEKSL 374

BLAST of Cp4.1LG09g01300.1 vs. NCBI nr
Match: KAG6574139.1 (Gibberellin 20 oxidase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 724 bits (1870), Expect = 1.48e-257
Identity = 354/374 (94.65%), Postives = 357/374 (95.45%), Query Frame = 0

Query: 3   MALNCMNATESSPALNHVKNREYRPAFCGSDETKIPEDFIWPEEFKAAEVVPELHVPHID 62
           MALNCMNA ESS ALN VKNREYRPAFCGSDE+KIPEDFIWPEEFKAAEVVPELHVPHID
Sbjct: 1   MALNCMNAAESSLALNQVKNREYRPAFCGSDESKIPEDFIWPEEFKAAEVVPELHVPHID 60

Query: 63  LKKFFSGNESDVEEATRLVDEACRKHGFFVVVNHGVDMELMKGVHECIDEFFTMPLDVKQ 122
           LKKFFSGNESDVEEATRLVDEACRKHGFFVVVNHGVDMELMKGVHECIDEFFTMPLDVKQ
Sbjct: 61  LKKFFSGNESDVEEATRLVDEACRKHGFFVVVNHGVDMELMKGVHECIDEFFTMPLDVKQ 120

Query: 123 RAQRKVGENYGYTNSFIGRFASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGSPFSHHGK 182
           RAQRKVGENYGYTNSFIGRFASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGS FSHHGK
Sbjct: 121 RAQRKVGENYGYTNSFIGRFASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHGK 180

Query: 183 LYQECGIALNELGMKIVELLGLSLGISRECFKNFYEDNDSILRLNYYPTCDKPELVLGTG 242
           LYQECGIALNELGMKIVELLGLSLGISRECFKNFYEDNDSILRLNYYPTC+KPELVLGTG
Sbjct: 181 LYQECGIALNELGMKIVELLGLSLGISRECFKNFYEDNDSILRLNYYPTCEKPELVLGTG 240

Query: 243 PHSDPTSITILHQDHVSGLQVFVDDQWYSIPPTPDSF---------SLTNGIYKSCLHRA 302
           PHSDPTSITILHQDHVSGLQVFVDDQWYSIPPTPDSF         SLTNGIYKSCLHRA
Sbjct: 241 PHSDPTSITILHQDHVSGLQVFVDDQWYSIPPTPDSFVINIGDTFMSLTNGIYKSCLHRA 300

Query: 303 VVNCKESRKSLAFFLCPAHDKVVRAPEELVEKNPPRNYSDYTWEMLLKMTQKDYRADFKT 362
           VVNCKESRKSLAFFLCPAHDKVVRAPEELVEK PPRNYSDYTWEMLLKMTQKDYRADFKT
Sbjct: 301 VVNCKESRKSLAFFLCPAHDKVVRAPEELVEKYPPRNYSDYTWEMLLKMTQKDYRADFKT 360

Query: 363 LEAFMARMATESSL 367
           LEAFMAR+  E SL
Sbjct: 361 LEAFMARVQKEKSL 374

BLAST of Cp4.1LG09g01300.1 vs. NCBI nr
Match: XP_022968301.1 (gibberellin 20 oxidase 1-like [Cucurbita maxima])

HSP 1 Score: 713 bits (1840), Expect = 5.35e-253
Identity = 345/374 (92.25%), Postives = 354/374 (94.65%), Query Frame = 0

Query: 3   MALNCMNATESSPALNHVKNREYRPAFCGSDETKIPEDFIWPEEFKAAEVVPELHVPHID 62
           MALNCMNATESSPALN VKNREY PAFCGSDE+KIPE+FIWPEEFKAAEVVPELHVPHID
Sbjct: 1   MALNCMNATESSPALNQVKNREYLPAFCGSDESKIPEEFIWPEEFKAAEVVPELHVPHID 60

Query: 63  LKKFFSGNESDVEEATRLVDEACRKHGFFVVVNHGVDMELMKGVHECIDEFFTMPLDVKQ 122
            KKFFSGNESD+EEATRLVDEACRKHGFFV+VNHGVDMELMK VHEC++EFFTMPLDVKQ
Sbjct: 61  FKKFFSGNESDIEEATRLVDEACRKHGFFVLVNHGVDMELMKAVHECMNEFFTMPLDVKQ 120

Query: 123 RAQRKVGENYGYTNSFIGRFASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGSPFSHHGK 182
           RA RKVGENYGYTNSFIGRFASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGS FSHHGK
Sbjct: 121 RAGRKVGENYGYTNSFIGRFASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHGK 180

Query: 183 LYQECGIALNELGMKIVELLGLSLGISRECFKNFYEDNDSILRLNYYPTCDKPELVLGTG 242
           LYQECGIALNELGMKIVELLGLSLGISRECFKNFYEDNDSILRLNYYPTC KPELVLGTG
Sbjct: 181 LYQECGIALNELGMKIVELLGLSLGISRECFKNFYEDNDSILRLNYYPTCGKPELVLGTG 240

Query: 243 PHSDPTSITILHQDHVSGLQVFVDDQWYSIPPTPDSF---------SLTNGIYKSCLHRA 302
           PHSDPTS+TILHQDHVSGLQVFVDDQWYSIPPTPDSF         SLTNGIYKSCLHRA
Sbjct: 241 PHSDPTSVTILHQDHVSGLQVFVDDQWYSIPPTPDSFVINIGDTFMSLTNGIYKSCLHRA 300

Query: 303 VVNCKESRKSLAFFLCPAHDKVVRAPEELVEKNPPRNYSDYTWEMLLKMTQKDYRADFKT 362
           VVNCKESRKSLAFFLCPAHDKVVRAPEELVEKNPPRNY DYTWEMLL+MTQKDYRADFKT
Sbjct: 301 VVNCKESRKSLAFFLCPAHDKVVRAPEELVEKNPPRNYPDYTWEMLLEMTQKDYRADFKT 360

Query: 363 LEAFMARMATESSL 367
           LEAFMAR+  E SL
Sbjct: 361 LEAFMARVQKEKSL 374

BLAST of Cp4.1LG09g01300.1 vs. ExPASy TrEMBL
Match: A0A6J1G194 (gibberellin 20 oxidase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111449754 PE=3 SV=1)

HSP 1 Score: 730 bits (1884), Expect = 5.35e-260
Identity = 355/374 (94.92%), Postives = 359/374 (95.99%), Query Frame = 0

Query: 3   MALNCMNATESSPALNHVKNREYRPAFCGSDETKIPEDFIWPEEFKAAEVVPELHVPHID 62
           MALNCMNATESSPALN V+NREYRPAFCGSDE+KIPEDFIWPEEFKAAEVVPELHVPHID
Sbjct: 1   MALNCMNATESSPALNQVRNREYRPAFCGSDESKIPEDFIWPEEFKAAEVVPELHVPHID 60

Query: 63  LKKFFSGNESDVEEATRLVDEACRKHGFFVVVNHGVDMELMKGVHECIDEFFTMPLDVKQ 122
           LKKFFSGNESDVEEATRLVDEACRKHGFFVVVNHGVDMELMKGVHECIDEFFTMPLDVKQ
Sbjct: 61  LKKFFSGNESDVEEATRLVDEACRKHGFFVVVNHGVDMELMKGVHECIDEFFTMPLDVKQ 120

Query: 123 RAQRKVGENYGYTNSFIGRFASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGSPFSHHGK 182
           RAQRKVGENYGYTNSFIGRFASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGS FSHHGK
Sbjct: 121 RAQRKVGENYGYTNSFIGRFASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHGK 180

Query: 183 LYQECGIALNELGMKIVELLGLSLGISRECFKNFYEDNDSILRLNYYPTCDKPELVLGTG 242
           LYQECGIALNELGMKIVELLGLSLGISRECFKNFYEDNDSILRLNYYP C+KPELVLGTG
Sbjct: 181 LYQECGIALNELGMKIVELLGLSLGISRECFKNFYEDNDSILRLNYYPICEKPELVLGTG 240

Query: 243 PHSDPTSITILHQDHVSGLQVFVDDQWYSIPPTPDSF---------SLTNGIYKSCLHRA 302
           PHSDPTSITILHQDHVSGLQVFVDDQWYSIPPTPDSF         SLTNGIYKSCLHRA
Sbjct: 241 PHSDPTSITILHQDHVSGLQVFVDDQWYSIPPTPDSFVINIGDTFMSLTNGIYKSCLHRA 300

Query: 303 VVNCKESRKSLAFFLCPAHDKVVRAPEELVEKNPPRNYSDYTWEMLLKMTQKDYRADFKT 362
           VVNCKESRKSLAFFLCPAHDKVVRAPEELVEKNPPRNYSDYTWEMLLKMTQKDYRADFKT
Sbjct: 301 VVNCKESRKSLAFFLCPAHDKVVRAPEELVEKNPPRNYSDYTWEMLLKMTQKDYRADFKT 360

Query: 363 LEAFMARMATESSL 367
           LEAFMAR+  E SL
Sbjct: 361 LEAFMARVQKEKSL 374

BLAST of Cp4.1LG09g01300.1 vs. ExPASy TrEMBL
Match: A0A6J1HUH4 (gibberellin 20 oxidase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467572 PE=3 SV=1)

HSP 1 Score: 713 bits (1840), Expect = 2.59e-253
Identity = 345/374 (92.25%), Postives = 354/374 (94.65%), Query Frame = 0

Query: 3   MALNCMNATESSPALNHVKNREYRPAFCGSDETKIPEDFIWPEEFKAAEVVPELHVPHID 62
           MALNCMNATESSPALN VKNREY PAFCGSDE+KIPE+FIWPEEFKAAEVVPELHVPHID
Sbjct: 1   MALNCMNATESSPALNQVKNREYLPAFCGSDESKIPEEFIWPEEFKAAEVVPELHVPHID 60

Query: 63  LKKFFSGNESDVEEATRLVDEACRKHGFFVVVNHGVDMELMKGVHECIDEFFTMPLDVKQ 122
            KKFFSGNESD+EEATRLVDEACRKHGFFV+VNHGVDMELMK VHEC++EFFTMPLDVKQ
Sbjct: 61  FKKFFSGNESDIEEATRLVDEACRKHGFFVLVNHGVDMELMKAVHECMNEFFTMPLDVKQ 120

Query: 123 RAQRKVGENYGYTNSFIGRFASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGSPFSHHGK 182
           RA RKVGENYGYTNSFIGRFASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGS FSHHGK
Sbjct: 121 RAGRKVGENYGYTNSFIGRFASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHGK 180

Query: 183 LYQECGIALNELGMKIVELLGLSLGISRECFKNFYEDNDSILRLNYYPTCDKPELVLGTG 242
           LYQECGIALNELGMKIVELLGLSLGISRECFKNFYEDNDSILRLNYYPTC KPELVLGTG
Sbjct: 181 LYQECGIALNELGMKIVELLGLSLGISRECFKNFYEDNDSILRLNYYPTCGKPELVLGTG 240

Query: 243 PHSDPTSITILHQDHVSGLQVFVDDQWYSIPPTPDSF---------SLTNGIYKSCLHRA 302
           PHSDPTS+TILHQDHVSGLQVFVDDQWYSIPPTPDSF         SLTNGIYKSCLHRA
Sbjct: 241 PHSDPTSVTILHQDHVSGLQVFVDDQWYSIPPTPDSFVINIGDTFMSLTNGIYKSCLHRA 300

Query: 303 VVNCKESRKSLAFFLCPAHDKVVRAPEELVEKNPPRNYSDYTWEMLLKMTQKDYRADFKT 362
           VVNCKESRKSLAFFLCPAHDKVVRAPEELVEKNPPRNY DYTWEMLL+MTQKDYRADFKT
Sbjct: 301 VVNCKESRKSLAFFLCPAHDKVVRAPEELVEKNPPRNYPDYTWEMLLEMTQKDYRADFKT 360

Query: 363 LEAFMARMATESSL 367
           LEAFMAR+  E SL
Sbjct: 361 LEAFMARVQKEKSL 374

BLAST of Cp4.1LG09g01300.1 vs. ExPASy TrEMBL
Match: A0A6J1G185 (gibberellin 20 oxidase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111449755 PE=3 SV=1)

HSP 1 Score: 670 bits (1729), Expect = 2.08e-236
Identity = 322/343 (93.88%), Postives = 335/343 (97.67%), Query Frame = 0

Query: 356 AFMARMATESSLSAHSNLNQEMKRERRETFGGSEESKVPEYFIWPDEFKGSQLVPELHVP 415
           AF  +MAT+SSLSAH NLNQEMKRERRETFGGS+ S VPEYFIWPDEFKGSQL+PELHVP
Sbjct: 2   AFNCKMATKSSLSAHLNLNQEMKRERRETFGGSDGSMVPEYFIWPDEFKGSQLLPELHVP 61

Query: 416 HIDVNKFLSGNESDVEEATRLVDEACREHGFFVVVNHGVDMELMKGVHECMNEFFTMPLD 475
           HID+NKFLSG+ESDVEEATRLVDEACREHGFFVVVNHGVDMELMKGVHEC++EFFTMPLD
Sbjct: 62  HIDLNKFLSGDESDVEEATRLVDEACREHGFFVVVNHGVDMELMKGVHECIDEFFTMPLD 121

Query: 476 VKQRAKRKVGESYGYTNSFFGRFASKLPWKETFSLRCVAAQNSSTARDYVIDILGSTFSH 535
           VKQRA+RKVGE+YGYTNSFFGRFASKLPWKETFSLRCVAAQNSSTARDYVID LGSTFSH
Sbjct: 122 VKQRAQRKVGENYGYTNSFFGRFASKLPWKETFSLRCVAAQNSSTARDYVIDTLGSTFSH 181

Query: 536 HGKVYQECGKALNDLGMKILEILGLSLGVSREYFKNFYEDNDSILRLNYYPTCDKPEVVL 595
           HGKVYQECGKALNDLGMKILEILGLSLGVSREYFKNFYEDNDSILRLNYYP C+KPEVVL
Sbjct: 182 HGKVYQECGKALNDLGMKILEILGLSLGVSREYFKNFYEDNDSILRLNYYPPCEKPEVVL 241

Query: 596 GTGPHTDPTSVTILHQDHVSGLQVCVNDQWYSIPPTPDSFVVNIGDTFTSLTNGIYKSCI 655
           GTGPHTDPTSVTILHQDHVSGLQVCVNDQWYSIPPTPDSFVVNIGDTFTSLTNGIYKSC+
Sbjct: 242 GTGPHTDPTSVTILHQDHVSGLQVCVNDQWYSIPPTPDSFVVNIGDTFTSLTNGIYKSCV 301

Query: 656 HRAVVNCKEARKSMAFSLCPAHDKVVRAPEELVEKNPPRKYPD 698
           HRAVVNCKEARKSMAF +CPAHDKVVRAPEELVEKNPPRKYPD
Sbjct: 302 HRAVVNCKEARKSMAFFICPAHDKVVRAPEELVEKNPPRKYPD 344

BLAST of Cp4.1LG09g01300.1 vs. ExPASy TrEMBL
Match: A0A6J1HZ92 (gibberellin 20 oxidase 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467571 PE=3 SV=1)

HSP 1 Score: 568 bits (1464), Expect = 2.91e-196
Identity = 275/343 (80.17%), Postives = 305/343 (88.92%), Query Frame = 0

Query: 356 AFMARMATESSLSAHSNLNQEMKRERRETFGGSEESKVPEYFIWPDEFKGSQLVPELHVP 415
           A   ++ATES   A SNLN+EMK E R  FGGS+ESKVPE FIW ++F+ S+L+P L VP
Sbjct: 2   ALNGKVATES---APSNLNEEMKGEYRPPFGGSDESKVPEDFIWSEKFEASELLPVLDVP 61

Query: 416 HIDVNKFLSGNESDVEEATRLVDEACREHGFFVVVNHGVDMELMKGVHECMNEFFTMPLD 475
            ID+ KF+SG++S VEEATRLVDEACR+HG F VVNHGVD+E+M  VH+CMNEFFTMPLD
Sbjct: 62  TIDLEKFMSGDKSYVEEATRLVDEACRQHGIFFVVNHGVDIEMMGRVHDCMNEFFTMPLD 121

Query: 476 VKQRAKRKVGESYGYTNSFFGRFASKLPWKETFSLRCVAAQNSSTARDYVIDILGSTFSH 535
           VKQRAKRKVGESYGYTNSFFGRFAS LPWKETFSLRCVAAQNSS A DYV+D LGS+FSH
Sbjct: 122 VKQRAKRKVGESYGYTNSFFGRFASNLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSH 181

Query: 536 HGKVYQECGKALNDLGMKILEILGLSLGVSREYFKNFYEDNDSILRLNYYPTCDKPEVVL 595
           HGK YQECG ALN+LG KI+E+LGLSLG+SREYFKNFY+DNDSILRLNYYPTCDKPEVVL
Sbjct: 182 HGKAYQECGIALNELGTKIVELLGLSLGISREYFKNFYKDNDSILRLNYYPTCDKPEVVL 241

Query: 596 GTGPHTDPTSVTILHQDHVSGLQVCVNDQWYSIPPTPDSFVVNIGDTFTSLTNGIYKSCI 655
           GTGPHTDPTSVTILHQD VSGLQVC NDQWYSIPP P++FV+NIGDTFTSLTNGIYK CI
Sbjct: 242 GTGPHTDPTSVTILHQDPVSGLQVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYKGCI 301

Query: 656 HRAVVNCKEARKSMAFSLCPAHDKVVRAPEELVEKNPPRKYPD 698
           HRAVVN   ARKS+AF LCP+HDKVVRAPEELVEK+PPRKYPD
Sbjct: 302 HRAVVNSMNARKSLAFFLCPSHDKVVRAPEELVEKSPPRKYPD 341

BLAST of Cp4.1LG09g01300.1 vs. ExPASy TrEMBL
Match: A0A6J1HXL8 (gibberellin 20 oxidase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467570 PE=3 SV=1)

HSP 1 Score: 568 bits (1464), Expect = 2.91e-196
Identity = 275/343 (80.17%), Postives = 305/343 (88.92%), Query Frame = 0

Query: 356 AFMARMATESSLSAHSNLNQEMKRERRETFGGSEESKVPEYFIWPDEFKGSQLVPELHVP 415
           A   ++ATES   A SNLN+EMK E R  FGGS+ESKVPE FIW ++F+ S+L+P L VP
Sbjct: 2   ALNGKVATES---APSNLNEEMKGEYRPPFGGSDESKVPEDFIWSEKFEASELLPVLDVP 61

Query: 416 HIDVNKFLSGNESDVEEATRLVDEACREHGFFVVVNHGVDMELMKGVHECMNEFFTMPLD 475
            ID+ KF+SG++S VEEATRLVDEACR+HG F VVNHGVD+E+M  VH+CMNEFFTMPLD
Sbjct: 62  TIDLEKFMSGDKSYVEEATRLVDEACRQHGIFFVVNHGVDIEMMGRVHDCMNEFFTMPLD 121

Query: 476 VKQRAKRKVGESYGYTNSFFGRFASKLPWKETFSLRCVAAQNSSTARDYVIDILGSTFSH 535
           VKQRAKRKVGESYGYTNSFFGRFAS LPWKETFSLRCVAAQNSS A DYV+D LGS+FSH
Sbjct: 122 VKQRAKRKVGESYGYTNSFFGRFASNLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSH 181

Query: 536 HGKVYQECGKALNDLGMKILEILGLSLGVSREYFKNFYEDNDSILRLNYYPTCDKPEVVL 595
           HGK YQECG ALN+LG KI+E+LGLSLG+SREYFKNFY+DNDSILRLNYYPTCDKPEVVL
Sbjct: 182 HGKAYQECGIALNELGTKIVELLGLSLGISREYFKNFYKDNDSILRLNYYPTCDKPEVVL 241

Query: 596 GTGPHTDPTSVTILHQDHVSGLQVCVNDQWYSIPPTPDSFVVNIGDTFTSLTNGIYKSCI 655
           GTGPHTDPTSVTILHQD VSGLQVC NDQWYSIPP P++FV+NIGDTFTSLTNGIYK CI
Sbjct: 242 GTGPHTDPTSVTILHQDPVSGLQVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYKGCI 301

Query: 656 HRAVVNCKEARKSMAFSLCPAHDKVVRAPEELVEKNPPRKYPD 698
           HRAVVN   ARKS+AF LCP+HDKVVRAPEELVEK+PPRKYPD
Sbjct: 302 HRAVVNSMNARKSLAFFLCPSHDKVVRAPEELVEKSPPRKYPD 341

BLAST of Cp4.1LG09g01300.1 vs. TAIR 10
Match: AT4G25420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 382.1 bits (980), Expect = 9.5e-106
Identity = 186/334 (55.69%), Postives = 230/334 (68.86%), Query Frame = 0

Query: 34  ETKIPEDFIWPEEFKAAEVVPELHVPHIDLKKFFSGNESDVEEATRLVDEACRKHGFFVV 93
           +  IP  FIWP++ K +  V EL VP IDL+   S + S   +A+RL+ EAC+KHGFF+V
Sbjct: 37  QANIPNQFIWPDDEKPSINVLELDVPLIDLQNLLS-DPSSTLDASRLISEACKKHGFFLV 96

Query: 94  VNHGVDMELMKGVHECIDEFFTMPLDVKQRAQRKVGENYGYTNSFIGRFASKLPWKETFS 153
           VNHG+  EL+   HE    FF MPL  KQR  RK GE+ GY +SF GRF++KLPWKET S
Sbjct: 97  VNHGISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLS 156

Query: 154 LR-CVAAQNSSAAHDYVLDTLGSPFSHHGKLYQECGIALNELGMKIVELLGLSLGISREC 213
            R C     S +  DY  D LG  F   GK+YQE   A++ L +KI+ELLGLSLG+ R+ 
Sbjct: 157 FRFCDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDY 216

Query: 214 FKNFYEDNDSILRLNYYPTCDKPELVLGTGPHSDPTSITILHQDHVSGLQVFVDDQWYSI 273
           F+ F+E+NDSI+RLNYYP C KP+L LGTGPH DPTS+TILHQDHV+GLQVFV++QW SI
Sbjct: 217 FREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSI 276

Query: 274 PPTPDSF---------SLTNGIYKSCLHRAVVNCKESRKSLAFFLCPAHDKVVRAPEELV 333
            P P +F         +L+N  YKSCLHRAVVN +  RKSLAFFLCP  D+VV  P EL+
Sbjct: 277 RPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRELL 336

Query: 334 EKNPPRNYSDYTWEMLLKMTQKDYRADFKTLEAF 358
           +    R Y D+TW M L+ TQK YRAD  TL+AF
Sbjct: 337 DSITSRRYPDFTWSMFLEFTQKHYRADMNTLQAF 369

BLAST of Cp4.1LG09g01300.1 vs. TAIR 10
Match: AT5G51810.1 (gibberellin 20 oxidase 2 )

HSP 1 Score: 380.2 bits (975), Expect = 3.6e-105
Identity = 182/343 (53.06%), Postives = 241/343 (70.26%), Query Frame = 0

Query: 34  ETKIPEDFIWPEEFKAAEVVPELHVPHIDLKKFFSGNESDVEEATRLVDEACRKHGFFVV 93
           +++IP  FIWP+E K +  +PEL+VP IDL      ++    EA R++ EAC KHGFF+V
Sbjct: 39  QSQIPNQFIWPDEEKPSIDIPELNVPFIDL-----SSQDSTLEAPRVIAEACTKHGFFLV 98

Query: 94  VNHGVDMELMKGVHECIDEFFTMPLDVKQRAQRKVGENYGYTNSFIGRFASKLPWKETFS 153
           VNHGV   L+   H  ++ FF MPL  KQ+AQRK GE+ GY +SF GRF++KLPWKET S
Sbjct: 99  VNHGVSESLIADAHRLMESFFDMPLAGKQKAQRKPGESCGYASSFTGRFSTKLPWKETLS 158

Query: 154 LRCVAAQNSS-AAHDYVLDTLGSPFSHHGKLYQECGIALNELGMKIVELLGLSLGISREC 213
            +     + S    DY  DTLG  F   GK+YQ+   A++ L +KI+ELLGLSLG++R+ 
Sbjct: 159 FQFSNDNSGSRTVQDYFSDTLGQEFEQFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRDY 218

Query: 214 FKNFYEDNDSILRLNYYPTCDKPELVLGTGPHSDPTSITILHQDHVSGLQVFVDDQWYSI 273
           F+ F+E+NDSI+RLN+YP C  P+L LGTGPH DP+S+TILHQDHV+GLQVFVD+QW SI
Sbjct: 219 FRGFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQDHVNGLQVFVDNQWQSI 278

Query: 274 PPTPDSF---------SLTNGIYKSCLHRAVVNCKESRKSLAFFLCPAHDKVVRAPEELV 333
            P P +F         +L+NGI+KSCLHRAVVN + +RKS+AFFLCP  DKVV+ P +++
Sbjct: 279 RPNPKAFVVNIGDTFMALSNGIFKSCLHRAVVNRESARKSMAFFLCPKKDKVVKPPSDIL 338

Query: 334 EKNPPRNYSDYTWEMLLKMTQKDYRADFKTLEAFMARMATESS 367
           EK   R Y D+TW M L+ TQK YRAD  TL++F   + T ++
Sbjct: 339 EKMKTRKYPDFTWSMFLEFTQKHYRADVNTLDSFSNWVITNNN 376

BLAST of Cp4.1LG09g01300.1 vs. TAIR 10
Match: AT5G07200.1 (gibberellin 20-oxidase 3 )

HSP 1 Score: 372.5 bits (955), Expect = 7.6e-103
Identity = 182/334 (54.49%), Postives = 226/334 (67.66%), Query Frame = 0

Query: 37  IPEDFIWPEEFKAAEVVPELHVPHIDLKKFFSGNESDVEEATRLVDEACRKHGFFVVVNH 96
           IP+ F+WP+  K +  V  L VP IDL  F SG+     EATRLV +A  KHGFF++ NH
Sbjct: 37  IPQQFVWPDHEKPSTDVQPLQVPLIDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNH 96

Query: 97  GVDMELMKGVHECIDEFFTMPLDVKQRAQRKVGENYGYTNSFIGRFASKLPWKETFSLRC 156
           GVD  L+   +  +D FF  P   KQ+AQRK GE+ GY +SF+GRF+SKLPWKET S + 
Sbjct: 97  GVDESLLSRAYLHMDSFFKAPACEKQKAQRKWGESSGYASSFVGRFSSKLPWKETLSFKF 156

Query: 157 VAAQ--NSSAAHDYVLDTLGSPFSHHGKLYQECGIALNELGMKIVELLGLSLGISRECFK 216
              +  +S    D+V   +G  +   GK+YQE   A+N L +KI+ELLG+SLG+ R  FK
Sbjct: 157 SPEEKIHSQTVKDFVSKKMGDGYEDFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFK 216

Query: 217 NFYEDNDSILRLNYYPTCDKPELVLGTGPHSDPTSITILHQDHVSGLQVFVDDQWYSIPP 276
            F+ED+DSI RLNYYP C +PEL LGTGPH DPTS+TILHQD V GLQVFVD++W SIPP
Sbjct: 217 EFFEDSDSIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPP 276

Query: 277 TPDSF---------SLTNGIYKSCLHRAVVNCKESRKSLAFFLCPAHDKVVRAPEELVE- 336
            P +F         +LTNG YKSCLHRAVVN +  RK+ AFFLCP  +KVV+ PEELV  
Sbjct: 277 NPHAFVVNIGDTFMALTNGRYKSCLHRAVVNSERERKTFAFFLCPKGEKVVKPPEELVNG 336

Query: 337 -KNPPRNYSDYTWEMLLKMTQKDYRADFKTLEAF 358
            K+  R Y D+TW M L+ TQK YRAD  TL+ F
Sbjct: 337 VKSGERKYPDFTWSMFLEFTQKHYRADMNTLDEF 370

BLAST of Cp4.1LG09g01300.1 vs. TAIR 10
Match: AT1G60980.1 (gibberellin 20-oxidase 4 )

HSP 1 Score: 344.0 bits (881), Expect = 2.9e-94
Identity = 172/334 (51.50%), Postives = 222/334 (66.47%), Query Frame = 0

Query: 372 NLNQEMKRERR---ETFGGSEESKVPEYFIWPDEFKGSQLVPELHVPHIDVNKFLSGNES 431
           N N+  K   R    T    +   +P+ F+WPD  K S+ VP L VP ID+  FLS +  
Sbjct: 12  NKNKSKKNPLRIFDSTVLNHQPDHIPQEFVWPDHEKPSKNVPILQVPVIDLAGFLSNDPL 71

Query: 432 DVEEATRLVDEACREHGFFVVVNHGVDMELMKGVHECMNEFFTMPLDVKQRAKRKVGESY 491
            V EA RLV EA ++HGFF+V NHGVD  L+   H+ M+ FF  P   K +A+RKVGE+ 
Sbjct: 72  LVSEAERLVSEAAKKHGFFLVTNHGVDERLLSTAHKLMDTFFKSPNYEKLKAQRKVGETT 131

Query: 492 GYTNSFFGRFASKLPWKETFSLRCVAAQN----SSTARDYVIDILGSTFSHHGKVYQECG 551
           GY +SF GRF   LPWKET S      +     S T ++Y+   +G  +   G VYQE  
Sbjct: 132 GYASSFVGRFKENLPWKETLSFSFSPTEKSENYSQTVKNYISKTMGDGYKDFGSVYQEYA 191

Query: 552 KALNDLGMKILEILGLSLGVSREYFKNFYEDNDSILRLNYYPTCDKPEVVLGTGPHTDPT 611
           + +++L +KI+E+LG+SLG+ RE+F+ F+EDN+SI RLNYYP C +P++VLGTGPH DPT
Sbjct: 192 ETMSNLSLKIMELLGMSLGIKREHFREFFEDNESIFRLNYYPKCKQPDLVLGTGPHCDPT 251

Query: 612 SVTILHQDHVSGLQVCVNDQWYSIPPTPDSFVVNIGDTFTSLTNGIYKSCIHRAVVNCKE 671
           S+TIL QD VSGLQV V++QW SIPP P + VVNIGDT  +LTNGIYKSC+HRAVVN + 
Sbjct: 252 SLTILQQDQVSGLQVFVDNQWQSIPPIPQALVVNIGDTLMALTNGIYKSCLHRAVVNGET 311

Query: 672 ARKSMAFSLCPAHDKVVRAPEELVEKNPPRKYPD 699
            RK++AF LCP  DKVV+ P EL      R YPD
Sbjct: 312 TRKTLAFFLCPKVDKVVKPPSEL---EGERAYPD 342

BLAST of Cp4.1LG09g01300.1 vs. TAIR 10
Match: AT1G44090.1 (gibberellin 20-oxidase 5 )

HSP 1 Score: 259.6 bits (662), Expect = 7.1e-69
Identity = 134/314 (42.68%), Postives = 188/314 (59.87%), Query Frame = 0

Query: 390 ESKVPEYFIWPDEFKGSQLVP---ELHVPHIDVNKFLSGNESDVEEATRLVDEACREHGF 449
           +  VP  F WP++     + P   +L +P ID++ FL+GNE++ + A + V +AC  HG 
Sbjct: 40  QPNVPAEFFWPEK----DVAPSEGDLDLPIIDLSGFLNGNEAETQLAAKAVKKACMAHGT 99

Query: 450 FVVVNHGVDMELMKGVHECMNEFFTMPLDVKQRAKRKVGESYGYTNSFFGRFASKLPWKE 509
           F+VVNHG    L +   E  + FF +  D K RA R  G   GYT     RF+S LPW E
Sbjct: 100 FLVVNHGFKSGLAEKALEISSLFFGLSKDEKLRAYRIPGNISGYTAGHSQRFSSNLPWNE 159

Query: 510 TFSLRCVAAQNSSTARDYVIDILGSTFSHHGKVYQECGKALNDLGMKILEILGLSLGV-S 569
           T +L            D++   LG+     G+V+QE   A+N L M ++E+LG+S+G+  
Sbjct: 160 TLTL-AFKKGPPHVVEDFLTSRLGNHRQEIGQVFQEFCDAMNGLVMDLMELLGISMGLKD 219

Query: 570 REYFKNFYEDNDSILRLNYYPTCDKPEVVLGTGPHTDPTSVTILHQDHVSGLQVCVNDQW 629
           R Y++ F+ED   I R NYYP C +PE  LG GPH DPT++T+L QD V GL+V     W
Sbjct: 220 RTYYRRFFEDGSGIFRCNYYPPCKQPEKALGVGPHNDPTAITVLLQDDVVGLEVFAAGSW 279

Query: 630 YSIPPTPDSFVVNIGDTFTSLTNGIYKSCIHRAVVNCKEARKSMAFSLCPAHDKVVRAPE 689
            ++ P P + VVN+GDTF +L+NG Y+SC HRAVVN ++ R+S+ F  CP  DK++  P 
Sbjct: 280 QTVRPRPGALVVNVGDTFMALSNGNYRSCYHRAVVNKEKVRRSLVFFSCPREDKIIVPPP 339

Query: 690 ELVE-KNPPRKYPD 699
           ELVE +   RKYPD
Sbjct: 340 ELVEGEEASRKYPD 348

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q391101.3e-10455.69Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2[more]
Q391115.1e-10453.06Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1[more]
Q391121.1e-10154.49Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=GA20OX3 PE=2 SV=1[more]
O047078.4e-9952.75Gibberellin 20 oxidase 1-A OS=Triticum aestivum OX=4565 GN=GA20ox1A PE=2 SV=1[more]
O047068.4e-9953.87Gibberellin 20 oxidase 1-B OS=Triticum aestivum OX=4565 GN=GA20ox1B PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023542034.10.068.28uncharacterized protein LOC111802006 [Cucurbita pepo subsp. pepo][more]
XP_023541911.11.07e-26496.52gibberellin 20 oxidase 1-like [Cucurbita pepo subsp. pepo][more]
XP_022945557.11.11e-25994.92gibberellin 20 oxidase 1-like [Cucurbita moschata][more]
KAG6574139.11.48e-25794.65Gibberellin 20 oxidase 2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022968301.15.35e-25392.25gibberellin 20 oxidase 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1G1945.35e-26094.92gibberellin 20 oxidase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111449754 PE=3... [more]
A0A6J1HUH42.59e-25392.25gibberellin 20 oxidase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467572 PE=3 S... [more]
A0A6J1G1852.08e-23693.88gibberellin 20 oxidase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111449755 PE=3... [more]
A0A6J1HZ922.91e-19680.17gibberellin 20 oxidase 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11146... [more]
A0A6J1HXL82.91e-19680.17gibberellin 20 oxidase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467570 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT4G25420.19.5e-10655.692-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G51810.13.6e-10553.06gibberellin 20 oxidase 2 [more]
AT5G07200.17.6e-10354.49gibberellin 20-oxidase 3 [more]
AT1G60980.12.9e-9451.50gibberellin 20-oxidase 4 [more]
AT1G44090.17.1e-6942.68gibberellin 20-oxidase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00682IPNSYNTHASEcoord: 600..621
score: 40.08
coord: 635..661
score: 32.66
coord: 437..454
score: 56.57
NoneNo IPR availablePANTHERPTHR47990:SF26GIBBERELLIN 20-OXIDASEcoord: 385..698
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 29..359
coord: 385..698
NoneNo IPR availablePANTHERPTHR47990:SF26GIBBERELLIN 20-OXIDASEcoord: 29..359
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 30..349
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 387..697
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 28..349
e-value: 2.7E-78
score: 265.6
coord: 383..698
e-value: 3.3E-85
score: 288.3
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 221..310
e-value: 7.2E-20
score: 71.4
coord: 577..674
e-value: 1.2E-25
score: 89.9
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 414..518
e-value: 9.4E-23
score: 81.2
coord: 58..162
e-value: 1.0E-23
score: 84.3
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 219..310
score: 13.032937
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 575..675
score: 13.004821

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cp4.1LG09g01300Cp4.1LG09g01300gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG09g01300.1:exon:001Cp4.1LG09g01300.1:exon:001exon
Cp4.1LG09g01300.1:exon:002Cp4.1LG09g01300.1:exon:002exon
Cp4.1LG09g01300.1:exon:003Cp4.1LG09g01300.1:exon:003exon
Cp4.1LG09g01300.1:exon:004Cp4.1LG09g01300.1:exon:004exon
Cp4.1LG09g01300.1:exon:005Cp4.1LG09g01300.1:exon:005exon
Cp4.1LG09g01300.1:exon:006Cp4.1LG09g01300.1:exon:006exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG09g01300.1:cds:001Cp4.1LG09g01300.1:cds:001CDS
Cp4.1LG09g01300.1:cds:002Cp4.1LG09g01300.1:cds:002CDS
Cp4.1LG09g01300.1:cds:003Cp4.1LG09g01300.1:cds:003CDS
Cp4.1LG09g01300.1:cds:004Cp4.1LG09g01300.1:cds:004CDS
Cp4.1LG09g01300.1:cds:005Cp4.1LG09g01300.1:cds:005CDS
Cp4.1LG09g01300.1:cds:006Cp4.1LG09g01300.1:cds:006CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cp4.1LG09g01300.1Cp4.1LG09g01300.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016707 gibberellin 3-beta-dioxygenase activity
molecular_function GO:0046872 metal ion binding