Cp4.1LG06g10130.1 (mRNA) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG06g10130.1
TypemRNA
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionAB hydrolase-1 domain-containing protein
LocationCp4.1LG06: 10455004 .. 10459182 (-)
Sequence length2264
RNA-Seq ExpressionCp4.1LG06g10130.1
SyntenyCp4.1LG06g10130.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATTCTTAACACACGGATTCATCATTAAAAGTCTTGCAAGGTTGGAAGCCTCAACTCCGCCGCACGACCCTTTTCTTCCTTTCTTACTCTCACCCACTCCCGCTTACTAAACTCTTGACAATACCGCGATGAACACAACCCCTAATTCCTCGCCTTAACCTTCGTTTTTCATGAGATTTCTTCGATTTCTTATCGTTTTGAGTCGAAAAAAATTCTGGGTTTTGTTCAGAATCAGGTTCTGAATAATGGGTAACAGCCTGATTTGCAAGTCAAAGAAGGATGTGAAAGAGATTGGATCAAGAAGCAAGAGAATGGGTCGGTCGCAGAGGAAGTTGCAGAGCGAGGAAGAGTTTTTGCAGAAGCAAGCACTCTCGTTGGCTTTGCAACAGCTTCAATTGTCTCAGAGGTTTGATGGATCCACTTCGAAAAGAATTGGGTCGACAAGTTCTCGAAGACATAATCTATCCGATCCATTCTGTAATGGGAAGCAGGTGGGTCTTCATTTTGTTTGTTTGTTTGTTTGTTTATATTTGGATCAAGAAAAGTGGGTTATCATGATTAAGCTCTGTTTGATTTCATGGCGGATTGCCTTTTAGAATTGTTCATATTTTATAATCTTTGTGAGTCTACTTTTCAGCTTTAAATAGTTTCTTTTTCCTTTGTGGTTGACCCTTTCTGGCTATCATTACTCTGTTCTTTTTGAGAATGAATGATCTCTGGTGCTTAATTATAGACGTCAGAATCTACCATGTGATGTAAATTATGAAAAGTCTACCAAATCCATGAATAGAAAGTAATAAAGGAAGAACTATGAAAAGTAATCAATGAGTAGGATCTTAATGAGTTCTAGACCTCGTGAGCTATGGTTTGATTCGAACCCCTATGAAGTTTTGACCATTTGTTAGAGAAAAGAGAACGAACGAATGGATCTTATAGAACGAATGGATCTCATGTTCCGTGAATAGCTGGGACATTAAGGAATACNGGGACATTGAGGAATATCCAGAAAAGCATTTAGGGAATCGGTCTGATTCTATCTCTGTTCGTTCCGTTTGAAGAAATGAAGGATCTATTGCATTAACACGATTTTCTTTTCTTCCTCTTAGAATCAGAGCAAGAGAGATTCGAGAGCAGAGGTGACTTCAGGTGAAGCAAATCTGCAGGTGAGGCTTAGAAATGTCTACCTGTTATCAGATTCTGCTACTTTATCACTAATCATCTCACCAGTTCATATCATTTTGCAGGCACTAGATTTTCTTGAAAACGTAAAGGAAAAGAAATTTGTTTTAGTTCATGGTGAAGGGTTTGGAGCTTGGTGTTGGTACAAAACAATCTCTCTACTGGAGGAAGTCGGATTGTCTCCAATAGCCATTGATCTCAAAGGCTCTGGTATTGATCTAACAGATACAAACAAGGTGGATACTCTAGTTGAGTACTCAAAGCCATTGATTGAGTATCTACAAGGCCTTCCTGACAACGAAAAGGTATTAGTGAAGTATCAGAGCTACAAGGCCTTCTAACAGAGTACTTTACCCGCTCATTCTGCTAATTAAAGCTAACAAAACTTGGTGTTTTGAAGGTTGTTCTAGTTGGGCACAGTAGTGGAGGTGCTTGTGTTTCTTATGCTTTAGAACATTTCTCAAACAAAATATCGAAAGCAGTTTACATTTGTGCAACGATGGTGGGTGATGGGCAGAGACCTTTTGATGTGTTCATGGAAGAGGTATTTGATTAACTATGGAACGTTGCTTTTCTTGGAGTTTCAGAAGCTCTCTGTTGTTCATGCTGATGATATAAACTGATTTGCAGCTTGGATCTGAAGAGATTTTTATGAAAGGCTCGAAGTTTTTGATCTATGGGAATGGAAAAGATGAACAGCCTACAGGATTCATGTTTGAGAAAGAGCAAATGAAGGGGTTGTACTTCAATCAATCTCCTACCAAGGTACTTTTCTCACCTTGGCTTTAAATCGAGTTTGATTTAGGATCCTTTTGTATACATTCGTAGTTTCTTATGCTTTAGAGTACCGATGAAGTATCAGAGCTGGGTGATGTTCATGAGCTCCAAGTTTGGTTTAGGATCCTTTTGTATACATTCGTAGTTTCTTCTGCTTTAGAGTACTAGTAAAGTATCAGAGCTAGATGATGTTCATGAGCTCCAAGTTTAGTTTAGGATCCTATTGGTAGTTTCTTATGCTTTAGAGTACTAGTGAAGTAACAAAGATAGGTGATGTTCATGAACTTCGAGTTTGGTTTATGATCCTTTCATGTACATTTGTAGTTTCTCTTGCCTTGGAGTACTAGTGAAGTATTAGAGCTAGGTGATGTTCATGAGCTTCAAGTTTGGTTTAGAATCCTTTCGTATACGTTCATAGTTTCTTCTGCTTTAGAGTACTAGTGAAGTATTAGAGTTAGGTGATGTTCATGAGCTCCGAATTTGGTTAGGATCCTTTCGTATACATTCGTAGTTTCTTCTGCTTTAGTGTACTAGTGAAGTATCAGAGCTAGGTGATGTTCATGAGCTCTGAGTTTGGTTTGGGATTCTTTTGTATACACTCGTAGTTTCTTTTGCTTTTAGAGTACTAGTGAAGTATCATAGCTAGGTAATGTTCCTGAGCTCCAAGTTTGGTTTAGAACCTCTCAGAATACTAGTGAAGTATCAAAGCTAGATGATGTTCAAGATCCTTTCGTATGCACTTGTAGTTTCTTATGCTTTAGAGTACTAGTGAAATATCAGAGCTAGGTGATGTTCGTGAACTCCGAGTTTGGTTTAGGATCCTACTCATAATAGTTTCTTCTACTTTAGAGTACTGGCAACCAAGCTTCTCCATAGAAAACTTGCTCGTTGAAGTTTGTTTTATGCTTCTGAGTTGTGTAAATATATATGAAGATGAGCTAAAGAGCAATATGGTTGGTGGGAATGCAGGATGTGGCTTTGGCAATGGTTTCAATGAGACCCTTCCCATTGGGTCCAGTCATGGAGAAACTCTCACTGTCCCCTGAAAACTACGAAACCGGGCGTCGATTCTTTGTTCAAACGCTCGATGATCACGCACTCTCACCTGATATACAAGAAAAGCTGGTCAGGGAAAACCCACCTGAAAGAGTTTTCAAGATCAAAGGCAGTGATCATTGCCCTTTTTTCTCTAAGCCACAATCACTCCACAAGATCCTCCTGGAAATTGCTCAAATACCATAAACTCTAGGCTTCTCACTCTCTCAATCTGTCTCATAGAAAGAACAAAACAAAGCTTTTTTGTTCCCTTGATTTGATTTTGTAAGTTATTTATTTATATATAGCAGAACCAAAAAAGATCACAGGGCATAATAAAATCATGGATTTGTGCTATGAATGAATATCAGAAAGCAAAGAACACGAGGTTCTTATCCAGAAATGACCAAACAAACACAAAGGGAAGTAAATAAAAGTGATATATTAGTCGATAGAAACACATATATCATAATCATCCATCACATTTTTCATATACGACATGAAAGAGTATTAAGTTTGATGATTCTGATTCACAGCCCCACGCAAAGAATCAAGTACAATTGTGTACTAACCACCCCCAGTGAGATTATGGTAAGCAGAGTACTACTAGGTACTTTCTATACTTCAAGCTCCTAAGGGAAAAGAAACATAGCATTATGACCAAAACATCAAGCAAAGGGAAAGTTAGAATGAATCTCCCTCTTGGCTTTGATCTCGTGTCACGCAAGCCACGTATGTATATACCAGCACGTTCCTCCAGGTGAAGCAGTGTCACACTTGGTATGTTTGGGCAAAAAAATCAGATCATACCAAACCAAAATCCACAAACCATATTTGGTCTTCAACCAAAGAAACTTCGATTTTGTAAAACCAGAAATCCATAGAACGCATATTCGGTCTTCAACCAGAGAACACTTCTGTGTTTTGTAACCACCCGTATACGAAACAATTGCATCTTGCAATCCAGTTCATGAAACCTATTACTGTTACCAACTGCTTCCAGGGCATATCTCCATTTTACATGATGATGCCTTCTGAGGATGTGCATTAAGTCCTCAGAATGGAGATATTCCCCCTGGAAGTTTGAGGAACCATAGTCCTGTCTGGATGCCATTATCTGTTCGATAGCATACACATCGTCACGATCCAATAGAGAACAATAAATAAAACAGCACACAAAATCCAGCC

mRNA sequence

CATTCTTAACACACGGATTCATCATTAAAAGTCTTGCAAGGTTGGAAGCCTCAACTCCGCCGCACGACCCTTTTCTTCCTTTCTTACTCTCACCCACTCCCGCTTACTAAACTCTTGACAATACCGCGATGAACACAACCCCTAATTCCTCGCCTTAACCTTCGTTTTTCATGAGATTTCTTCGATTTCTTATCGTTTTGAGTCGAAAAAAATTCTGGGTTTTGTTCAGAATCAGGTTCTGAATAATGGGTAACAGCCTGATTTGCAAGTCAAAGAAGGATGTGAAAGAGATTGGATCAAGAAGCAAGAGAATGGGTCGGTCGCAGAGGAAGTTGCAGAGCGAGGAAGAGTTTTTGCAGAAGCAAGCACTCTCGTTGGCTTTGCAACAGCTTCAATTGTCTCAGAGGTTTGATGGATCCACTTCGAAAAGAATTGGGTCGACAAGTTCTCGAAGACATAATCTATCCGATCCATTCTGTAATGGGAAGCAGGCACTAGATTTTCTTGAAAACGTAAAGGAAAAGAAATTTGTTTTAGTTCATGGTGAAGGGTTTGGAGCTTGGTGTTGGTACAAAACAATCTCTCTACTGGAGGAAGTCGGATTGTCTCCAATAGCCATTGATCTCAAAGGCTCTGGTATTGATCTAACAGATACAAACAAGGTGGATACTCTAGTTGAGTACTCAAAGCCATTGATTGAGTATCTACAAGGCCTTCCTGACAACGAAAAGGTTGTTCTAGTTGGGCACAGTAGTGGAGGTGCTTGTGTTTCTTATGCTTTAGAACATTTCTCAAACAAAATATCGAAAGCAGTTTACATTTGTGCAACGATGGTGGGTGATGGGCAGAGACCTTTTGATGTGTTCATGGAAGAGCTTGGATCTGAAGAGATTTTTATGAAAGGCTCGAAGTTTTTGATCTATGGGAATGGAAAAGATGAACAGCCTACAGGATTCATGTTTGAGAAAGAGCAAATGAAGGGGTTGTACTTCAATCAATCTCCTACCAAGGATGTGGCTTTGGCAATGGTTTCAATGAGACCCTTCCCATTGGGTCCAGTCATGGAGAAACTCTCACTGTCCCCTGAAAACTACGAAACCGGGCGTCGATTCTTTGTTCAAACGCTCGATGATCACGCACTCTCACCTGATATACAAGAAAAGCTGGTCAGGGAAAACCCACCTGAAAGAGTTTTCAAGATCAAAGGCAGTGATCATTGCCCTTTTTTCTCTAAGCCACAATCACTCCACAAGATCCTCCTGGAAATTGCTCAAATACCATAAACTCTAGGCTTCTCACTCTCTCAATCTGTCTCATAGAAAGAACAAAACAAAGCTTTTTTGTTCCCTTGATTTGATTTTGTAAGTTATTTATTTATATATAGCAGAACCAAAAAAGATCACAGGGCATAATAAAATCATGGATTTGTGCTATGAATGAATATCAGAAAGCAAAGAACACGAGGTTCTTATCCAGAAATGACCAAACAAACACAAAGGGAAGTAAATAAAAGTGATATATTAGTCGATAGAAACACATATATCATAATCATCCATCACATTTTTCATATACGACATGAAAGAGTATTAAGTTTGATGATTCTGATTCACAGCCCCACGCAAAGAATCAAGTACAATTGTGTACTAACCACCCCCAGTGAGATTATGGTAAGCAGAGTACTACTAGGTACTTTCTATACTTCAAGCTCCTAAGGGAAAAGAAACATAGCATTATGACCAAAACATCAAGCAAAGGGAAAGTTAGAATGAATCTCCCTCTTGGCTTTGATCTCGTGTCACGCAAGCCACGTATGTATATACCAGCACGTTCCTCCAGGTGAAGCAGTGTCACACTTGGTATGTTTGGGCAAAAAAATCAGATCATACCAAACCAAAATCCACAAACCATATTTGGTCTTCAACCAAAGAAACTTCGATTTTGTAAAACCAGAAATCCATAGAACGCATATTCGGTCTTCAACCAGAGAACACTTCTGTGTTTTGTAACCACCCGTATACGAAACAATTGCATCTTGCAATCCAGTTCATGAAACCTATTACTGTTACCAACTGCTTCCAGGGCATATCTCCATTTTACATGATGATGCCTTCTGAGGATGTGCATTAAGTCCTCAGAATGGAGATATTCCCCCTGGAAGTTTGAGGAACCATAGTCCTGTCTGGATGCCATTATCTGTTCGATAGCATACACATCGTCACGATCCAATAGAGAACAATAAATAAAACAGCACACAAAATCCAGCC

Coding sequence (CDS)

ATGGGTAACAGCCTGATTTGCAAGTCAAAGAAGGATGTGAAAGAGATTGGATCAAGAAGCAAGAGAATGGGTCGGTCGCAGAGGAAGTTGCAGAGCGAGGAAGAGTTTTTGCAGAAGCAAGCACTCTCGTTGGCTTTGCAACAGCTTCAATTGTCTCAGAGGTTTGATGGATCCACTTCGAAAAGAATTGGGTCGACAAGTTCTCGAAGACATAATCTATCCGATCCATTCTGTAATGGGAAGCAGGCACTAGATTTTCTTGAAAACGTAAAGGAAAAGAAATTTGTTTTAGTTCATGGTGAAGGGTTTGGAGCTTGGTGTTGGTACAAAACAATCTCTCTACTGGAGGAAGTCGGATTGTCTCCAATAGCCATTGATCTCAAAGGCTCTGGTATTGATCTAACAGATACAAACAAGGTGGATACTCTAGTTGAGTACTCAAAGCCATTGATTGAGTATCTACAAGGCCTTCCTGACAACGAAAAGGTTGTTCTAGTTGGGCACAGTAGTGGAGGTGCTTGTGTTTCTTATGCTTTAGAACATTTCTCAAACAAAATATCGAAAGCAGTTTACATTTGTGCAACGATGGTGGGTGATGGGCAGAGACCTTTTGATGTGTTCATGGAAGAGCTTGGATCTGAAGAGATTTTTATGAAAGGCTCGAAGTTTTTGATCTATGGGAATGGAAAAGATGAACAGCCTACAGGATTCATGTTTGAGAAAGAGCAAATGAAGGGGTTGTACTTCAATCAATCTCCTACCAAGGATGTGGCTTTGGCAATGGTTTCAATGAGACCCTTCCCATTGGGTCCAGTCATGGAGAAACTCTCACTGTCCCCTGAAAACTACGAAACCGGGCGTCGATTCTTTGTTCAAACGCTCGATGATCACGCACTCTCACCTGATATACAAGAAAAGCTGGTCAGGGAAAACCCACCTGAAAGAGTTTTCAAGATCAAAGGCAGTGATCATTGCCCTTTTTTCTCTAAGCCACAATCACTCCACAAGATCCTCCTGGAAATTGCTCAAATACCATAA

Protein sequence

MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEFLQKQALSLALQQLQLSQRFDGSTSKRIGSTSSRRHNLSDPFCNGKQALDFLENVKEKKFVLVHGEGFGAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDNEKVVLVGHSSGGACVSYALEHFSNKISKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGSKFLIYGNGKDEQPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPENYETGRRFFVQTLDDHALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIAQIP
Homology
BLAST of Cp4.1LG06g10130.1 vs. ExPASy Swiss-Prot
Match: Q940H7 (Putative methylesterase 12, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES12 PE=2 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 1.3e-145
Identity = 258/349 (73.93%), Postives = 290/349 (83.09%), Query Frame = 0

Query: 1   MGNSLICKSKKDV----KEIGSRSKRMGRSQRKLQSEEEFLQKQALSLALQQLQLSQRFD 60
           MGN +IC  KKDV       GSRSKR+ RSQRKL ++EE L ++ALS+A+ Q Q+SQRFD
Sbjct: 1   MGNRVICMKKKDVVIRSGGDGSRSKRVNRSQRKLLADEENLHRRALSMAIHQAQVSQRFD 60

Query: 61  GSTSKRIGSTSSRRHNLSDPFCNGKQALDFLENVKEKKFVLVHGEGFGAWCWYKTISLLE 120
           GS S+RIGSTSSRR  LSD F N KQ  +FLE++K KKFVLVHGEGFGAWCWYKTI+ LE
Sbjct: 61  GSMSRRIGSTSSRRGTLSDSFSNNKQVPEFLESLKVKKFVLVHGEGFGAWCWYKTIASLE 120

Query: 121 EVGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDNEKVVLVGHSSGGACVS 180
           E GLSP+ +DL GSG ++TD N V TL EYSKPLIE +Q LP  EKV+LVGHS+GGACVS
Sbjct: 121 ESGLSPVTVDLAGSGFNMTDANSVSTLEEYSKPLIELIQNLPAEEKVILVGHSTGGACVS 180

Query: 181 YALEHFSNKISKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGSKFLIYGNGKDEQPTG 240
           YALE F  KISKA++ICATMV DGQRPFDVF +ELGS E FMK S+FLIYGNGKD   TG
Sbjct: 181 YALERFPEKISKAIFICATMVTDGQRPFDVFADELGSAERFMKESQFLIYGNGKDNPATG 240

Query: 241 FMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPENYETGRRFFVQTLDD 300
           FMFEK+ MKGLYFNQSP KD+AL+M+SMRP PLGP+MEKLSLS E Y  GRRF+VQTLDD
Sbjct: 241 FMFEKQHMKGLYFNQSPNKDIALSMISMRPVPLGPMMEKLSLSAERYGKGRRFYVQTLDD 300

Query: 301 HALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIAQIP 346
            ALSPD+QEKLVREN PE VFKIKGSDHCPFFSKPQSLHKILLEIAQIP
Sbjct: 301 LALSPDVQEKLVRENSPEAVFKIKGSDHCPFFSKPQSLHKILLEIAQIP 349

BLAST of Cp4.1LG06g10130.1 vs. ExPASy Swiss-Prot
Match: Q9FVW3 (Putative methylesterase 14, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES14 PE=1 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 1.1e-144
Identity = 251/348 (72.13%), Postives = 290/348 (83.33%), Query Frame = 0

Query: 1   MGNSLICKSKKDVKE---IGSRSKRMGRSQRKLQSEEEFLQKQALSLALQQLQLSQRFDG 60
           MGN +I   KKD K+    GS+SKRM RSQRKL ++EE L ++ALS+A+ Q QLSQRFDG
Sbjct: 1   MGNKIISMMKKDSKDGGGGGSKSKRMNRSQRKLLADEEMLHRRALSMAIHQAQLSQRFDG 60

Query: 61  STSKRIGSTSSRRHNLSDPFCNGKQALDFLENVKEKKFVLVHGEGFGAWCWYKTISLLEE 120
           S S+R+GSTS+R+  LSDPF NGKQ  DF E++  KKFVLVHGEGFGAWCWYK ++ LEE
Sbjct: 61  SMSRRVGSTSTRKRTLSDPFSNGKQVPDFSESLIVKKFVLVHGEGFGAWCWYKMVASLEE 120

Query: 121 VGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDNEKVVLVGHSSGGACVSY 180
            GLSP+ +DL G G ++TDTN V TL EYSKPLI+ L+ LP+ EKV+LVGHS+GGA +SY
Sbjct: 121 SGLSPVTVDLTGCGFNMTDTNTVSTLEEYSKPLIDLLENLPEEEKVILVGHSTGGASISY 180

Query: 181 ALEHFSNKISKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGSKFLIYGNGKDEQPTGF 240
           ALE F  KISKA+++CATMV DGQRPFDVF EELGS E FMK S+FLIYGNGKD+ PTGF
Sbjct: 181 ALERFPEKISKAIFVCATMVSDGQRPFDVFSEELGSAERFMKESQFLIYGNGKDKPPTGF 240

Query: 241 MFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPENYETGRRFFVQTLDDH 300
           MFEK  MKGLYFNQSP KD+ALAM+SMRP PLGP+MEK+SL+ E Y  GRRF+VQTLDD 
Sbjct: 241 MFEKPHMKGLYFNQSPNKDIALAMISMRPVPLGPMMEKVSLTAERYGKGRRFYVQTLDDR 300

Query: 301 ALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIAQIP 346
           ALSPD+QEKLVREN PE VFKIKGSDHCPFFSKPQSLHKILLEIAQIP
Sbjct: 301 ALSPDVQEKLVRENSPEGVFKIKGSDHCPFFSKPQSLHKILLEIAQIP 348

BLAST of Cp4.1LG06g10130.1 vs. ExPASy Swiss-Prot
Match: F4IE65 (Putative methylesterase 13, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES13 PE=2 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 6.1e-82
Identity = 143/292 (48.97%), Postives = 208/292 (71.23%), Query Frame = 0

Query: 58  STSKRIGSTSSRRHNLSDPFCNGKQALD----FLENVKEKKFVLVHGEGFGAWCWYKTIS 117
           S++  + S+SSR+ + +DP     Q +D     +E  + K+FVLVHG GFGAWCWYKTI+
Sbjct: 149 SSAGFVRSSSSRQRSSTDPMIKPNQLVDKELNKVEGSETKRFVLVHGGGFGAWCWYKTIT 208

Query: 118 LLEEVGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDNEKVVLVGHSSGGA 177
           LLE+ G    A++L GSG+   DTN + +L  YSKPL+ + + L   EKV+LVGH  GGA
Sbjct: 209 LLEKHGFQVDAVELTGSGVSSIDTNNITSLAHYSKPLLHFFESLKPTEKVILVGHDFGGA 268

Query: 178 CVSYALEHFSNKISKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGSKFLIYGNGKDEQ 237
           C+SYA+E F  KI+KAV+I A M+ +GQ   D+F ++LGS ++ M+ ++  +Y NGK   
Sbjct: 269 CMSYAMEMFPTKIAKAVFISAAMLANGQSTLDLFNQQLGSNDL-MQQAQIFLYANGKKNP 328

Query: 238 PTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPENYETGRRFFVQT 297
           PT   F++  ++   FNQSP KD+ALA VS+RP P  PV EK+ +S +NY + RRF+++T
Sbjct: 329 PTAVDFDRSLLRDFLFNQSPPKDLALASVSIRPIPFAPVSEKVHVSEKNYGSIRRFYIKT 388

Query: 298 LDDHALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIAQIP 346
           ++D+A+   +QE +++ NPPE+VF++KGSDH PFFS+PQSL+KIL+EI+QIP
Sbjct: 389 MEDYAVPVLLQEAMIKLNPPEQVFQLKGSDHAPFFSRPQSLNKILVEISQIP 439

BLAST of Cp4.1LG06g10130.1 vs. ExPASy Swiss-Prot
Match: Q9FW03 (Putative methylesterase 11, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES11 PE=1 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 4.3e-80
Identity = 160/343 (46.65%), Postives = 224/343 (65.31%), Query Frame = 0

Query: 22  RMGRSQRKLQSEEEFLQKQALSLAL----QQ----------LQLSQRFDGSTSKR---IG 81
           R   S+R  + E+  +Q+ AL+ A     QQ             SQR+ GS SK+     
Sbjct: 46  RSSSSRRDNKFEDALIQEHALAAAAVLFRQQNGGGGSLPFDRSASQRYQGSCSKKNQLPR 105

Query: 82  STSSRRHNLSDPFCNGKQALD---FLENVKEKKFVLVHGEGFGAWCWYKTISLLEEVGLS 141
           S+SSR  + +DP     Q L+    L++++   FVLVHG  FGAWCWYKTI+LLEE G  
Sbjct: 106 SSSSRSRSSTDPLLQPHQFLNQGIKLDDLETNHFVLVHGGSFGAWCWYKTIALLEEDGFK 165

Query: 142 PIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDNEKVVLVGHSSGGACVSYALEH 201
             AIDL G GI+  + N + +L +Y KPL + L+ LP  EKV+LVGH  GGAC+SYA+E 
Sbjct: 166 VTAIDLAGCGINSININGIASLSQYVKPLTDILEKLPIGEKVILVGHDFGGACISYAMEL 225

Query: 202 FSNKISKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGSKFLIYGNGKDEQPTGFMFEK 261
           F +KISKAV++ A M+ +GQ   D+F  + G  ++ M+ ++  IY NG +  PT    +K
Sbjct: 226 FPSKISKAVFLAAAMLTNGQSTLDMFSLKAGQNDL-MRKAQIFIYTNGNENPPTAIDLDK 285

Query: 262 EQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPENYETGRRFFVQTLDDHALSP 321
             +K L FNQSP+KDVALA VSMR  P  PV+EKLSLS  NY + RR++++TL+D+A+  
Sbjct: 286 SLLKDLLFNQSPSKDVALASVSMRSIPFAPVLEKLSLSDANYGSVRRYYIETLEDNAIPV 345

Query: 322 DIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIAQI 345
            +QE ++  +PPE+V+++KG+DH PFFSKPQ+LHK+LLEIA+I
Sbjct: 346 TLQENMINSSPPEKVYRLKGADHAPFFSKPQALHKLLLEIARI 387

BLAST of Cp4.1LG06g10130.1 vs. ExPASy Swiss-Prot
Match: F4I0K9 (Putative methylesterase 15, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES15 PE=2 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 1.6e-79
Identity = 140/292 (47.95%), Postives = 201/292 (68.84%), Query Frame = 0

Query: 58  STSKRIGSTSSRRHNLSDPFCNGKQALD---FLENVKEKKFVLVHGEGFGAWCWYKTISL 117
           ST   + S+S+R+ + +DP     Q LD    +E  + K+FVLVHG GFGAWCWYKTI+L
Sbjct: 148 STYGYVRSSSNRQRSSTDPVLKPNQLLDKELKVEGAETKRFVLVHGGGFGAWCWYKTITL 207

Query: 118 LEEVGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDNEKVVLVGHSSGGAC 177
           LE+ G    A+DL GSG+   DTN + +L +Y KPL+ +   L   EKV+LVGH  GGAC
Sbjct: 208 LEKHGFQVDAVDLTGSGVSSFDTNNITSLAQYVKPLLHFFDTLKPTEKVILVGHDFGGAC 267

Query: 178 VSYALEHFSNKISKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGSKFLIYGNGKDEQP 237
           +SYA+E + +KI+KA++I A M+ + Q   D+F ++  S    M+     +Y NGK   P
Sbjct: 268 MSYAMEMYPSKIAKAIFISAAMLANAQSTLDLFNQQPDSNYDLMEQVHLFLYANGKKNPP 327

Query: 238 TGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPENYETGRRFFVQTL 297
           T   F++  ++  +FNQSP KDVALA VSMRP P  PV+EKL +S +NY + RRF+++T+
Sbjct: 328 TAVDFDRSLLRDFFFNQSPPKDVALASVSMRPIPFAPVVEKLHVSEKNYGSIRRFYIKTM 387

Query: 298 -DDHALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIAQIP 346
            DD+A+   +Q+ +++ NPPE+VF +KGSDH PFFS+PQSL++IL+EI+Q+P
Sbjct: 388 EDDYAVPVSLQDAMIKSNPPEQVFHLKGSDHAPFFSRPQSLNRILVEISQLP 439

BLAST of Cp4.1LG06g10130.1 vs. NCBI nr
Match: XP_023535568.1 (putative methylesterase 12, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 678 bits (1749), Expect = 2.36e-245
Identity = 345/364 (94.78%), Postives = 345/364 (94.78%), Query Frame = 0

Query: 1   MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEFLQKQALSLALQQLQLSQRFDGSTS 60
           MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEFLQKQALSLALQQLQLSQRFDGSTS
Sbjct: 1   MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEFLQKQALSLALQQLQLSQRFDGSTS 60

Query: 61  KRIGSTSSRRHNLSDPFCNGKQ-------------------ALDFLENVKEKKFVLVHGE 120
           KRIGSTSSRRHNLSDPFCNGKQ                   ALDFLENVKEKKFVLVHGE
Sbjct: 61  KRIGSTSSRRHNLSDPFCNGKQNQSKRDSRAEVTSGEANLQALDFLENVKEKKFVLVHGE 120

Query: 121 GFGAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDNE 180
           GFGAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDNE
Sbjct: 121 GFGAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDNE 180

Query: 181 KVVLVGHSSGGACVSYALEHFSNKISKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGS 240
           KVVLVGHSSGGACVSYALEHFSNKISKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGS
Sbjct: 181 KVVLVGHSSGGACVSYALEHFSNKISKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGS 240

Query: 241 KFLIYGNGKDEQPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPE 300
           KFLIYGNGKDEQPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPE
Sbjct: 241 KFLIYGNGKDEQPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPE 300

Query: 301 NYETGRRFFVQTLDDHALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEI 345
           NYETGRRFFVQTLDDHALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEI
Sbjct: 301 NYETGRRFFVQTLDDHALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEI 360

BLAST of Cp4.1LG06g10130.1 vs. NCBI nr
Match: XP_022925472.1 (putative methylesterase 12, chloroplastic isoform X2 [Cucurbita moschata])

HSP 1 Score: 676 bits (1743), Expect = 1.80e-244
Identity = 343/362 (94.75%), Postives = 344/362 (95.03%), Query Frame = 0

Query: 1   MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEFLQKQALSLALQQLQLSQRFDGSTS 60
           MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEFLQKQALSLALQQLQLSQRFDGSTS
Sbjct: 1   MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEFLQKQALSLALQQLQLSQRFDGSTS 60

Query: 61  KRIGSTSSRRHNLSDPFCNGKQ-----------------ALDFLENVKEKKFVLVHGEGF 120
           KRIGSTSSRRHNLSDPFCNGKQ                 ALDFLENVKEKKFVLVHGEGF
Sbjct: 61  KRIGSTSSRRHNLSDPFCNGKQSKRDSRAEVTSGEANLQALDFLENVKEKKFVLVHGEGF 120

Query: 121 GAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDNEKV 180
           GAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDNEKV
Sbjct: 121 GAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDNEKV 180

Query: 181 VLVGHSSGGACVSYALEHFSNKISKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGSKF 240
           VLVGHSSGGACVSYALEHFSNK+SKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGSKF
Sbjct: 181 VLVGHSSGGACVSYALEHFSNKVSKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGSKF 240

Query: 241 LIYGNGKDEQPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPENY 300
           LIYGNGKDEQPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPENY
Sbjct: 241 LIYGNGKDEQPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPENY 300

Query: 301 ETGRRFFVQTLDDHALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIAQ 345
            TGRRFFVQTLDDHALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIAQ
Sbjct: 301 GTGRRFFVQTLDDHALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIAQ 360

BLAST of Cp4.1LG06g10130.1 vs. NCBI nr
Match: XP_022925471.1 (putative methylesterase 12, chloroplastic isoform X1 [Cucurbita moschata])

HSP 1 Score: 675 bits (1741), Expect = 3.91e-244
Identity = 343/364 (94.23%), Postives = 344/364 (94.51%), Query Frame = 0

Query: 1   MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEFLQKQALSLALQQLQLSQRFDGSTS 60
           MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEFLQKQALSLALQQLQLSQRFDGSTS
Sbjct: 1   MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEFLQKQALSLALQQLQLSQRFDGSTS 60

Query: 61  KRIGSTSSRRHNLSDPFCNGKQ-------------------ALDFLENVKEKKFVLVHGE 120
           KRIGSTSSRRHNLSDPFCNGKQ                   ALDFLENVKEKKFVLVHGE
Sbjct: 61  KRIGSTSSRRHNLSDPFCNGKQNQSKRDSRAEVTSGEANLQALDFLENVKEKKFVLVHGE 120

Query: 121 GFGAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDNE 180
           GFGAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDNE
Sbjct: 121 GFGAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDNE 180

Query: 181 KVVLVGHSSGGACVSYALEHFSNKISKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGS 240
           KVVLVGHSSGGACVSYALEHFSNK+SKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGS
Sbjct: 181 KVVLVGHSSGGACVSYALEHFSNKVSKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGS 240

Query: 241 KFLIYGNGKDEQPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPE 300
           KFLIYGNGKDEQPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPE
Sbjct: 241 KFLIYGNGKDEQPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPE 300

Query: 301 NYETGRRFFVQTLDDHALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEI 345
           NY TGRRFFVQTLDDHALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEI
Sbjct: 301 NYGTGRRFFVQTLDDHALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEI 360

BLAST of Cp4.1LG06g10130.1 vs. NCBI nr
Match: KAG6592387.1 (putative methylesterase 12, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 674 bits (1738), Expect = 1.04e-243
Identity = 342/362 (94.48%), Postives = 344/362 (95.03%), Query Frame = 0

Query: 1   MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEFLQKQALSLALQQLQLSQRFDGSTS 60
           MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEFLQKQALSLALQQLQLSQRFDGSTS
Sbjct: 1   MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEFLQKQALSLALQQLQLSQRFDGSTS 60

Query: 61  KRIGSTSSRRHNLSDPFCNGKQ-----------------ALDFLENVKEKKFVLVHGEGF 120
           KRIGSTSSRRHNLSDPFCNGKQ                 ALDFLENVKEKKFVLVHGEGF
Sbjct: 61  KRIGSTSSRRHNLSDPFCNGKQSKRDSRAEVTSGEANLQALDFLENVKEKKFVLVHGEGF 120

Query: 121 GAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDNEKV 180
           GAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPD+EKV
Sbjct: 121 GAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDDEKV 180

Query: 181 VLVGHSSGGACVSYALEHFSNKISKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGSKF 240
           VLVGHSSGGACVSYALEHFSNK+SKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGSKF
Sbjct: 181 VLVGHSSGGACVSYALEHFSNKVSKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGSKF 240

Query: 241 LIYGNGKDEQPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPENY 300
           LIYGNGKDEQPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPENY
Sbjct: 241 LIYGNGKDEQPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPENY 300

Query: 301 ETGRRFFVQTLDDHALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIAQ 345
            TGRRFFVQTLDDHALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIAQ
Sbjct: 301 GTGRRFFVQTLDDHALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIAQ 360

BLAST of Cp4.1LG06g10130.1 vs. NCBI nr
Match: KAG7025202.1 (putative methylesterase 12, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 672 bits (1735), Expect = 2.98e-243
Identity = 341/362 (94.20%), Postives = 344/362 (95.03%), Query Frame = 0

Query: 1   MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEFLQKQALSLALQQLQLSQRFDGSTS 60
           MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEFLQKQALSLALQQLQLSQRFDGSTS
Sbjct: 1   MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEFLQKQALSLALQQLQLSQRFDGSTS 60

Query: 61  KRIGSTSSRRHNLSDPFCNGKQ-----------------ALDFLENVKEKKFVLVHGEGF 120
           KRIGSTSSRRHNLSDPFCNGKQ                 ALDFLENVKEKKFVLVHGEGF
Sbjct: 61  KRIGSTSSRRHNLSDPFCNGKQSKRDSRAEVTSGEGNLQALDFLENVKEKKFVLVHGEGF 120

Query: 121 GAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDNEKV 180
           GAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNKVDTLV+YSKPLIEYLQGLPD+EKV
Sbjct: 121 GAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNKVDTLVDYSKPLIEYLQGLPDDEKV 180

Query: 181 VLVGHSSGGACVSYALEHFSNKISKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGSKF 240
           VLVGHSSGGACVSYALEHFSNK+SKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGSKF
Sbjct: 181 VLVGHSSGGACVSYALEHFSNKVSKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGSKF 240

Query: 241 LIYGNGKDEQPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPENY 300
           LIYGNGKDEQPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPENY
Sbjct: 241 LIYGNGKDEQPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPENY 300

Query: 301 ETGRRFFVQTLDDHALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIAQ 345
            TGRRFFVQTLDDHALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIAQ
Sbjct: 301 GTGRRFFVQTLDDHALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIAQ 360

BLAST of Cp4.1LG06g10130.1 vs. ExPASy TrEMBL
Match: A0A6J1EBT1 (putative methylesterase 12, chloroplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111432764 PE=4 SV=1)

HSP 1 Score: 676 bits (1743), Expect = 8.71e-245
Identity = 343/362 (94.75%), Postives = 344/362 (95.03%), Query Frame = 0

Query: 1   MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEFLQKQALSLALQQLQLSQRFDGSTS 60
           MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEFLQKQALSLALQQLQLSQRFDGSTS
Sbjct: 1   MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEFLQKQALSLALQQLQLSQRFDGSTS 60

Query: 61  KRIGSTSSRRHNLSDPFCNGKQ-----------------ALDFLENVKEKKFVLVHGEGF 120
           KRIGSTSSRRHNLSDPFCNGKQ                 ALDFLENVKEKKFVLVHGEGF
Sbjct: 61  KRIGSTSSRRHNLSDPFCNGKQSKRDSRAEVTSGEANLQALDFLENVKEKKFVLVHGEGF 120

Query: 121 GAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDNEKV 180
           GAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDNEKV
Sbjct: 121 GAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDNEKV 180

Query: 181 VLVGHSSGGACVSYALEHFSNKISKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGSKF 240
           VLVGHSSGGACVSYALEHFSNK+SKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGSKF
Sbjct: 181 VLVGHSSGGACVSYALEHFSNKVSKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGSKF 240

Query: 241 LIYGNGKDEQPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPENY 300
           LIYGNGKDEQPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPENY
Sbjct: 241 LIYGNGKDEQPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPENY 300

Query: 301 ETGRRFFVQTLDDHALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIAQ 345
            TGRRFFVQTLDDHALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIAQ
Sbjct: 301 GTGRRFFVQTLDDHALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIAQ 360

BLAST of Cp4.1LG06g10130.1 vs. ExPASy TrEMBL
Match: A0A6J1ECB0 (putative methylesterase 12, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111432764 PE=4 SV=1)

HSP 1 Score: 675 bits (1741), Expect = 1.89e-244
Identity = 343/364 (94.23%), Postives = 344/364 (94.51%), Query Frame = 0

Query: 1   MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEFLQKQALSLALQQLQLSQRFDGSTS 60
           MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEFLQKQALSLALQQLQLSQRFDGSTS
Sbjct: 1   MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEFLQKQALSLALQQLQLSQRFDGSTS 60

Query: 61  KRIGSTSSRRHNLSDPFCNGKQ-------------------ALDFLENVKEKKFVLVHGE 120
           KRIGSTSSRRHNLSDPFCNGKQ                   ALDFLENVKEKKFVLVHGE
Sbjct: 61  KRIGSTSSRRHNLSDPFCNGKQNQSKRDSRAEVTSGEANLQALDFLENVKEKKFVLVHGE 120

Query: 121 GFGAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDNE 180
           GFGAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDNE
Sbjct: 121 GFGAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDNE 180

Query: 181 KVVLVGHSSGGACVSYALEHFSNKISKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGS 240
           KVVLVGHSSGGACVSYALEHFSNK+SKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGS
Sbjct: 181 KVVLVGHSSGGACVSYALEHFSNKVSKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGS 240

Query: 241 KFLIYGNGKDEQPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPE 300
           KFLIYGNGKDEQPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPE
Sbjct: 241 KFLIYGNGKDEQPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPE 300

Query: 301 NYETGRRFFVQTLDDHALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEI 345
           NY TGRRFFVQTLDDHALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEI
Sbjct: 301 NYGTGRRFFVQTLDDHALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEI 360

BLAST of Cp4.1LG06g10130.1 vs. ExPASy TrEMBL
Match: A0A6J1ICR7 (putative methylesterase 12, chloroplastic isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111472593 PE=4 SV=1)

HSP 1 Score: 659 bits (1700), Expect = 3.11e-238
Identity = 334/362 (92.27%), Postives = 341/362 (94.20%), Query Frame = 0

Query: 1   MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEFLQKQALSLALQQLQLSQRFDGSTS 60
           MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEFL+KQALSLALQQLQLSQRFDGSTS
Sbjct: 1   MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEFLKKQALSLALQQLQLSQRFDGSTS 60

Query: 61  KRIGSTSSRRHNLSDPFCNGKQ-----------------ALDFLENVKEKKFVLVHGEGF 120
           KRIGSTSSRRHNLSDPFCNGKQ                 ALDFLENVKEKKFVLVHGEGF
Sbjct: 61  KRIGSTSSRRHNLSDPFCNGKQSKRVSRAEVTSGEANLQALDFLENVKEKKFVLVHGEGF 120

Query: 121 GAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDNEKV 180
           GAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNKVD+LVEYSKPLIEYLQGLPDNEKV
Sbjct: 121 GAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNKVDSLVEYSKPLIEYLQGLPDNEKV 180

Query: 181 VLVGHSSGGACVSYALEHFSNKISKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGSKF 240
           VLVGHSSGGACVSYALEHFSNK++KAVYICATMVGDGQRPFDVFMEELGSEEIFMK SKF
Sbjct: 181 VLVGHSSGGACVSYALEHFSNKVTKAVYICATMVGDGQRPFDVFMEELGSEEIFMKDSKF 240

Query: 241 LIYGNGKDEQPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPENY 300
           LIYGNGKDE PTGFMFEKEQMKG+YFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSP+NY
Sbjct: 241 LIYGNGKDEPPTGFMFEKEQMKGMYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPKNY 300

Query: 301 ETGRRFFVQTLDDHALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIAQ 345
            TGRRFFVQTL+D ALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIAQ
Sbjct: 301 GTGRRFFVQTLNDRALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIAQ 360

BLAST of Cp4.1LG06g10130.1 vs. ExPASy TrEMBL
Match: A0A6J1IG72 (putative methylesterase 12, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111472593 PE=4 SV=1)

HSP 1 Score: 658 bits (1698), Expect = 6.77e-238
Identity = 334/364 (91.76%), Postives = 341/364 (93.68%), Query Frame = 0

Query: 1   MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEFLQKQALSLALQQLQLSQRFDGSTS 60
           MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEFL+KQALSLALQQLQLSQRFDGSTS
Sbjct: 1   MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEFLKKQALSLALQQLQLSQRFDGSTS 60

Query: 61  KRIGSTSSRRHNLSDPFCNGKQ-------------------ALDFLENVKEKKFVLVHGE 120
           KRIGSTSSRRHNLSDPFCNGKQ                   ALDFLENVKEKKFVLVHGE
Sbjct: 61  KRIGSTSSRRHNLSDPFCNGKQNPSKRVSRAEVTSGEANLQALDFLENVKEKKFVLVHGE 120

Query: 121 GFGAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDNE 180
           GFGAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNKVD+LVEYSKPLIEYLQGLPDNE
Sbjct: 121 GFGAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNKVDSLVEYSKPLIEYLQGLPDNE 180

Query: 181 KVVLVGHSSGGACVSYALEHFSNKISKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGS 240
           KVVLVGHSSGGACVSYALEHFSNK++KAVYICATMVGDGQRPFDVFMEELGSEEIFMK S
Sbjct: 181 KVVLVGHSSGGACVSYALEHFSNKVTKAVYICATMVGDGQRPFDVFMEELGSEEIFMKDS 240

Query: 241 KFLIYGNGKDEQPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPE 300
           KFLIYGNGKDE PTGFMFEKEQMKG+YFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSP+
Sbjct: 241 KFLIYGNGKDEPPTGFMFEKEQMKGMYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPK 300

Query: 301 NYETGRRFFVQTLDDHALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEI 345
           NY TGRRFFVQTL+D ALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEI
Sbjct: 301 NYGTGRRFFVQTLNDRALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEI 360

BLAST of Cp4.1LG06g10130.1 vs. ExPASy TrEMBL
Match: A0A1S3CA81 (putative methylesterase 12, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103498713 PE=4 SV=1)

HSP 1 Score: 631 bits (1627), Expect = 4.63e-227
Identity = 320/365 (87.67%), Postives = 333/365 (91.23%), Query Frame = 0

Query: 1   MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEFLQKQALSLALQQLQLSQRFDGSTS 60
           MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEE+LQKQALSLALQQLQLSQRFDGSTS
Sbjct: 1   MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEYLQKQALSLALQQLQLSQRFDGSTS 60

Query: 61  KRIGSTSSRRHNLSDPFCNGKQ--------------------ALDFLENVKEKKFVLVHG 120
           KRIGSTSSRR NLSDPF NGKQ                    A +FLEN+KEKKFVLVHG
Sbjct: 61  KRIGSTSSRRRNLSDPFSNGKQGKKDSKLCGADVASGEANVQAPEFLENLKEKKFVLVHG 120

Query: 121 EGFGAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDN 180
           EGFGAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTN+V+TL EYSKPL +YLQ LPD+
Sbjct: 121 EGFGAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNRVNTLAEYSKPLTDYLQDLPDD 180

Query: 181 EKVVLVGHSSGGACVSYALEHFSNKISKAVYICATMVGDGQRPFDVFMEELGSEEIFMKG 240
           EKVVLVGHSSGGAC+SYALEHFSNKISKA+Y+CATMV  GQRPFDVFMEELGSEEIFMK 
Sbjct: 181 EKVVLVGHSSGGACLSYALEHFSNKISKAIYVCATMVATGQRPFDVFMEELGSEEIFMKD 240

Query: 241 SKFLIYGNGKDEQPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSP 300
           SKFLIYGNGKD+ PTGFMFEKEQ+KGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSP
Sbjct: 241 SKFLIYGNGKDKPPTGFMFEKEQIKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSP 300

Query: 301 ENYETGRRFFVQTLDDHALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLE 345
           ENY TGRRFFVQTLDDHALSPD+QEKLVR NPPERVFKIKGSDHCPFFSKPQSLHKILLE
Sbjct: 301 ENYGTGRRFFVQTLDDHALSPDVQEKLVRVNPPERVFKIKGSDHCPFFSKPQSLHKILLE 360

BLAST of Cp4.1LG06g10130.1 vs. TAIR 10
Match: AT4G09900.1 (methyl esterase 12 )

HSP 1 Score: 517.3 bits (1331), Expect = 9.4e-147
Identity = 258/349 (73.93%), Postives = 290/349 (83.09%), Query Frame = 0

Query: 1   MGNSLICKSKKDV----KEIGSRSKRMGRSQRKLQSEEEFLQKQALSLALQQLQLSQRFD 60
           MGN +IC  KKDV       GSRSKR+ RSQRKL ++EE L ++ALS+A+ Q Q+SQRFD
Sbjct: 1   MGNRVICMKKKDVVIRSGGDGSRSKRVNRSQRKLLADEENLHRRALSMAIHQAQVSQRFD 60

Query: 61  GSTSKRIGSTSSRRHNLSDPFCNGKQALDFLENVKEKKFVLVHGEGFGAWCWYKTISLLE 120
           GS S+RIGSTSSRR  LSD F N KQ  +FLE++K KKFVLVHGEGFGAWCWYKTI+ LE
Sbjct: 61  GSMSRRIGSTSSRRGTLSDSFSNNKQVPEFLESLKVKKFVLVHGEGFGAWCWYKTIASLE 120

Query: 121 EVGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDNEKVVLVGHSSGGACVS 180
           E GLSP+ +DL GSG ++TD N V TL EYSKPLIE +Q LP  EKV+LVGHS+GGACVS
Sbjct: 121 ESGLSPVTVDLAGSGFNMTDANSVSTLEEYSKPLIELIQNLPAEEKVILVGHSTGGACVS 180

Query: 181 YALEHFSNKISKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGSKFLIYGNGKDEQPTG 240
           YALE F  KISKA++ICATMV DGQRPFDVF +ELGS E FMK S+FLIYGNGKD   TG
Sbjct: 181 YALERFPEKISKAIFICATMVTDGQRPFDVFADELGSAERFMKESQFLIYGNGKDNPATG 240

Query: 241 FMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPENYETGRRFFVQTLDD 300
           FMFEK+ MKGLYFNQSP KD+AL+M+SMRP PLGP+MEKLSLS E Y  GRRF+VQTLDD
Sbjct: 241 FMFEKQHMKGLYFNQSPNKDIALSMISMRPVPLGPMMEKLSLSAERYGKGRRFYVQTLDD 300

Query: 301 HALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIAQIP 346
            ALSPD+QEKLVREN PE VFKIKGSDHCPFFSKPQSLHKILLEIAQIP
Sbjct: 301 LALSPDVQEKLVRENSPEAVFKIKGSDHCPFFSKPQSLHKILLEIAQIP 349

BLAST of Cp4.1LG06g10130.1 vs. TAIR 10
Match: AT1G33990.1 (methyl esterase 14 )

HSP 1 Score: 514.2 bits (1323), Expect = 8.0e-146
Identity = 251/348 (72.13%), Postives = 290/348 (83.33%), Query Frame = 0

Query: 1   MGNSLICKSKKDVKE---IGSRSKRMGRSQRKLQSEEEFLQKQALSLALQQLQLSQRFDG 60
           MGN +I   KKD K+    GS+SKRM RSQRKL ++EE L ++ALS+A+ Q QLSQRFDG
Sbjct: 1   MGNKIISMMKKDSKDGGGGGSKSKRMNRSQRKLLADEEMLHRRALSMAIHQAQLSQRFDG 60

Query: 61  STSKRIGSTSSRRHNLSDPFCNGKQALDFLENVKEKKFVLVHGEGFGAWCWYKTISLLEE 120
           S S+R+GSTS+R+  LSDPF NGKQ  DF E++  KKFVLVHGEGFGAWCWYK ++ LEE
Sbjct: 61  SMSRRVGSTSTRKRTLSDPFSNGKQVPDFSESLIVKKFVLVHGEGFGAWCWYKMVASLEE 120

Query: 121 VGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDNEKVVLVGHSSGGACVSY 180
            GLSP+ +DL G G ++TDTN V TL EYSKPLI+ L+ LP+ EKV+LVGHS+GGA +SY
Sbjct: 121 SGLSPVTVDLTGCGFNMTDTNTVSTLEEYSKPLIDLLENLPEEEKVILVGHSTGGASISY 180

Query: 181 ALEHFSNKISKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGSKFLIYGNGKDEQPTGF 240
           ALE F  KISKA+++CATMV DGQRPFDVF EELGS E FMK S+FLIYGNGKD+ PTGF
Sbjct: 181 ALERFPEKISKAIFVCATMVSDGQRPFDVFSEELGSAERFMKESQFLIYGNGKDKPPTGF 240

Query: 241 MFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPENYETGRRFFVQTLDDH 300
           MFEK  MKGLYFNQSP KD+ALAM+SMRP PLGP+MEK+SL+ E Y  GRRF+VQTLDD 
Sbjct: 241 MFEKPHMKGLYFNQSPNKDIALAMISMRPVPLGPMMEKVSLTAERYGKGRRFYVQTLDDR 300

Query: 301 ALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIAQIP 346
           ALSPD+QEKLVREN PE VFKIKGSDHCPFFSKPQSLHKILLEIAQIP
Sbjct: 301 ALSPDVQEKLVRENSPEGVFKIKGSDHCPFFSKPQSLHKILLEIAQIP 348

BLAST of Cp4.1LG06g10130.1 vs. TAIR 10
Match: AT1G26360.1 (methyl esterase 13 )

HSP 1 Score: 305.8 bits (782), Expect = 4.3e-83
Identity = 143/292 (48.97%), Postives = 208/292 (71.23%), Query Frame = 0

Query: 58  STSKRIGSTSSRRHNLSDPFCNGKQALD----FLENVKEKKFVLVHGEGFGAWCWYKTIS 117
           S++  + S+SSR+ + +DP     Q +D     +E  + K+FVLVHG GFGAWCWYKTI+
Sbjct: 149 SSAGFVRSSSSRQRSSTDPMIKPNQLVDKELNKVEGSETKRFVLVHGGGFGAWCWYKTIT 208

Query: 118 LLEEVGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDNEKVVLVGHSSGGA 177
           LLE+ G    A++L GSG+   DTN + +L  YSKPL+ + + L   EKV+LVGH  GGA
Sbjct: 209 LLEKHGFQVDAVELTGSGVSSIDTNNITSLAHYSKPLLHFFESLKPTEKVILVGHDFGGA 268

Query: 178 CVSYALEHFSNKISKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGSKFLIYGNGKDEQ 237
           C+SYA+E F  KI+KAV+I A M+ +GQ   D+F ++LGS ++ M+ ++  +Y NGK   
Sbjct: 269 CMSYAMEMFPTKIAKAVFISAAMLANGQSTLDLFNQQLGSNDL-MQQAQIFLYANGKKNP 328

Query: 238 PTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPENYETGRRFFVQT 297
           PT   F++  ++   FNQSP KD+ALA VS+RP P  PV EK+ +S +NY + RRF+++T
Sbjct: 329 PTAVDFDRSLLRDFLFNQSPPKDLALASVSIRPIPFAPVSEKVHVSEKNYGSIRRFYIKT 388

Query: 298 LDDHALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIAQIP 346
           ++D+A+   +QE +++ NPPE+VF++KGSDH PFFS+PQSL+KIL+EI+QIP
Sbjct: 389 MEDYAVPVLLQEAMIKLNPPEQVFQLKGSDHAPFFSRPQSLNKILVEISQIP 439

BLAST of Cp4.1LG06g10130.1 vs. TAIR 10
Match: AT3G29770.1 (methyl esterase 11 )

HSP 1 Score: 299.7 bits (766), Expect = 3.1e-81
Identity = 160/343 (46.65%), Postives = 224/343 (65.31%), Query Frame = 0

Query: 22  RMGRSQRKLQSEEEFLQKQALSLAL----QQ----------LQLSQRFDGSTSKR---IG 81
           R   S+R  + E+  +Q+ AL+ A     QQ             SQR+ GS SK+     
Sbjct: 46  RSSSSRRDNKFEDALIQEHALAAAAVLFRQQNGGGGSLPFDRSASQRYQGSCSKKNQLPR 105

Query: 82  STSSRRHNLSDPFCNGKQALD---FLENVKEKKFVLVHGEGFGAWCWYKTISLLEEVGLS 141
           S+SSR  + +DP     Q L+    L++++   FVLVHG  FGAWCWYKTI+LLEE G  
Sbjct: 106 SSSSRSRSSTDPLLQPHQFLNQGIKLDDLETNHFVLVHGGSFGAWCWYKTIALLEEDGFK 165

Query: 142 PIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDNEKVVLVGHSSGGACVSYALEH 201
             AIDL G GI+  + N + +L +Y KPL + L+ LP  EKV+LVGH  GGAC+SYA+E 
Sbjct: 166 VTAIDLAGCGINSININGIASLSQYVKPLTDILEKLPIGEKVILVGHDFGGACISYAMEL 225

Query: 202 FSNKISKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGSKFLIYGNGKDEQPTGFMFEK 261
           F +KISKAV++ A M+ +GQ   D+F  + G  ++ M+ ++  IY NG +  PT    +K
Sbjct: 226 FPSKISKAVFLAAAMLTNGQSTLDMFSLKAGQNDL-MRKAQIFIYTNGNENPPTAIDLDK 285

Query: 262 EQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPENYETGRRFFVQTLDDHALSP 321
             +K L FNQSP+KDVALA VSMR  P  PV+EKLSLS  NY + RR++++TL+D+A+  
Sbjct: 286 SLLKDLLFNQSPSKDVALASVSMRSIPFAPVLEKLSLSDANYGSVRRYYIETLEDNAIPV 345

Query: 322 DIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIAQI 345
            +QE ++  +PPE+V+++KG+DH PFFSKPQ+LHK+LLEIA+I
Sbjct: 346 TLQENMINSSPPEKVYRLKGADHAPFFSKPQALHKLLLEIARI 387

BLAST of Cp4.1LG06g10130.1 vs. TAIR 10
Match: AT1G69240.1 (methyl esterase 15 )

HSP 1 Score: 297.7 bits (761), Expect = 1.2e-80
Identity = 140/292 (47.95%), Postives = 201/292 (68.84%), Query Frame = 0

Query: 58  STSKRIGSTSSRRHNLSDPFCNGKQALD---FLENVKEKKFVLVHGEGFGAWCWYKTISL 117
           ST   + S+S+R+ + +DP     Q LD    +E  + K+FVLVHG GFGAWCWYKTI+L
Sbjct: 148 STYGYVRSSSNRQRSSTDPVLKPNQLLDKELKVEGAETKRFVLVHGGGFGAWCWYKTITL 207

Query: 118 LEEVGLSPIAIDLKGSGIDLTDTNKVDTLVEYSKPLIEYLQGLPDNEKVVLVGHSSGGAC 177
           LE+ G    A+DL GSG+   DTN + +L +Y KPL+ +   L   EKV+LVGH  GGAC
Sbjct: 208 LEKHGFQVDAVDLTGSGVSSFDTNNITSLAQYVKPLLHFFDTLKPTEKVILVGHDFGGAC 267

Query: 178 VSYALEHFSNKISKAVYICATMVGDGQRPFDVFMEELGSEEIFMKGSKFLIYGNGKDEQP 237
           +SYA+E + +KI+KA++I A M+ + Q   D+F ++  S    M+     +Y NGK   P
Sbjct: 268 MSYAMEMYPSKIAKAIFISAAMLANAQSTLDLFNQQPDSNYDLMEQVHLFLYANGKKNPP 327

Query: 238 TGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPENYETGRRFFVQTL 297
           T   F++  ++  +FNQSP KDVALA VSMRP P  PV+EKL +S +NY + RRF+++T+
Sbjct: 328 TAVDFDRSLLRDFFFNQSPPKDVALASVSMRPIPFAPVVEKLHVSEKNYGSIRRFYIKTM 387

Query: 298 -DDHALSPDIQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIAQIP 346
            DD+A+   +Q+ +++ NPPE+VF +KGSDH PFFS+PQSL++IL+EI+Q+P
Sbjct: 388 EDDYAVPVSLQDAMIKSNPPEQVFHLKGSDHAPFFSRPQSLNRILVEISQLP 439

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q940H71.3e-14573.93Putative methylesterase 12, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES... [more]
Q9FVW31.1e-14472.13Putative methylesterase 14, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES... [more]
F4IE656.1e-8248.97Putative methylesterase 13, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES... [more]
Q9FW034.3e-8046.65Putative methylesterase 11, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES... [more]
F4I0K91.6e-7947.95Putative methylesterase 15, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES... [more]
Match NameE-valueIdentityDescription
XP_023535568.12.36e-24594.78putative methylesterase 12, chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_022925472.11.80e-24494.75putative methylesterase 12, chloroplastic isoform X2 [Cucurbita moschata][more]
XP_022925471.13.91e-24494.23putative methylesterase 12, chloroplastic isoform X1 [Cucurbita moschata][more]
KAG6592387.11.04e-24394.48putative methylesterase 12, chloroplastic, partial [Cucurbita argyrosperma subsp... [more]
KAG7025202.12.98e-24394.20putative methylesterase 12, chloroplastic, partial [Cucurbita argyrosperma subsp... [more]
Match NameE-valueIdentityDescription
A0A6J1EBT18.71e-24594.75putative methylesterase 12, chloroplastic isoform X2 OS=Cucurbita moschata OX=36... [more]
A0A6J1ECB01.89e-24494.23putative methylesterase 12, chloroplastic isoform X1 OS=Cucurbita moschata OX=36... [more]
A0A6J1ICR73.11e-23892.27putative methylesterase 12, chloroplastic isoform X2 OS=Cucurbita maxima OX=3661... [more]
A0A6J1IG726.77e-23891.76putative methylesterase 12, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661... [more]
A0A1S3CA814.63e-22787.67putative methylesterase 12, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10349871... [more]
Match NameE-valueIdentityDescription
AT4G09900.19.4e-14773.93methyl esterase 12 [more]
AT1G33990.18.0e-14672.13methyl esterase 14 [more]
AT1G26360.14.3e-8348.97methyl esterase 13 [more]
AT3G29770.13.1e-8146.65methyl esterase 11 [more]
AT1G69240.11.2e-8047.95methyl esterase 15 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 87..343
e-value: 7.5E-59
score: 201.6
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 95..341
IPR000073Alpha/beta hydrolase fold-1PFAMPF12697Abhydrolase_6coord: 95..332
e-value: 1.2E-16
score: 62.0
NoneNo IPR availablePANTHERPTHR10992METHYLESTERASE FAMILY MEMBERcoord: 25..344
NoneNo IPR availablePANTHERPTHR10992:SF785METHYLESTERASE 14, CHLOROPLASTIC-RELATEDcoord: 25..344

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cp4.1LG06g10130Cp4.1LG06g10130gene


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG06g10130.1:three_prime_utr:001Cp4.1LG06g10130.1:three_prime_utr:001three_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG06g10130.1:exon:005Cp4.1LG06g10130.1:exon:005exon
Cp4.1LG06g10130.1:exon:004Cp4.1LG06g10130.1:exon:004exon
Cp4.1LG06g10130.1:exon:003Cp4.1LG06g10130.1:exon:003exon
Cp4.1LG06g10130.1:exon:002Cp4.1LG06g10130.1:exon:002exon
Cp4.1LG06g10130.1:exon:001Cp4.1LG06g10130.1:exon:001exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG06g10130.1:cds:001Cp4.1LG06g10130.1:cds:001CDS
Cp4.1LG06g10130.1:cds:002Cp4.1LG06g10130.1:cds:002CDS
Cp4.1LG06g10130.1:cds:003Cp4.1LG06g10130.1:cds:003CDS
Cp4.1LG06g10130.1:cds:004Cp4.1LG06g10130.1:cds:004CDS
Cp4.1LG06g10130.1:cds:005Cp4.1LG06g10130.1:cds:005CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG06g10130.1:five_prime_utr:001Cp4.1LG06g10130.1:five_prime_utr:001five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cp4.1LG06g10130.1Cp4.1LG06g10130.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009694 jasmonic acid metabolic process
biological_process GO:0009696 salicylic acid metabolic process
molecular_function GO:0080030 methyl indole-3-acetate esterase activity
molecular_function GO:0080032 methyl jasmonate esterase activity
molecular_function GO:0080031 methyl salicylate esterase activity