Cp4.1LG04g12540.1 (mRNA) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG04g12540.1
TypemRNA
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionprotein FAF-like, chloroplastic
LocationCp4.1LG04: 7965463 .. 7967172 (+)
Sequence length1611
RNA-Seq ExpressionCp4.1LG04g12540.1
SyntenyCp4.1LG04g12540.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGGCCCTCTTCTCGATTCCCATGGCGGACTATAAATACCCACTTTCCTCTTCCCCTTTCGTGGCAACCTCATTGCATCATCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCACTTCCCCTGCTTCCCTTCTTTGCAACTCTGCACCTTCAGCGGTCTCCTTCTTCCGACCGCTGCCGGTTGCTGTAGTTGCTCTTCTAGAAACACTCAAAAAAACCCAACAACACAAAACCAAATCATGTCGCCATTGAAGATTTTTCAAGCTTCCTGCGACGACAAAAAACAGGGCATCGTCGCTGTTCTGTCCTCCGATCCCGACCGCTCCAAATCCCAACCTTCTTCTCTCCGAAGAACACTCTCTGCTGACATGTCGTCCCATAAATGGATTTCCCAAATGAAAAATGTCTCTTCTTGTCAAGAATTTTGTCTCTGGGATTCAATTCAAGAAAGGGAGAAAAAGAAGAAGAAAACAGAGGAGGATAAAACAGAGTCTCCTGATATTTGGGGCTCTGTCATCAAATCTCGCAAGGATTTTAATGCTAATCCTTATGTTCCTCCCCGGAAAATGGGTAAGTGTTTAAGTGAGAAAAGCCTCGAGATTTGTACTGAAAGTCTTGGATCGGAAACCGGCTCTGATGGGTTCTCGTCGTATCCATCATCGGAGAACGGAGATTCCGACGAGGAGGAGTTTGAAGTAGAGGATCAAGGGAAGCCGGTGCAGTATAGCTATAAAAAATCACCCCCCCGCCGGTCGTTTCCTCCCCCGTTGCCGACACTCTCCGGCCCGGATGGGGAGTTAGTACGGATGCAGCCCCGCCGTGACAACGGCCGGTTGGTCCTCGAGGCCATGTCCGTGCCTTCACAGAGCACCTTCTCCGCCCAACGTCAAGATGGCCGCCTCGTTCTCACCCTCGTTAATCGCAAGCCCATTAACGAGGTAAATCACTTAATTAGCAAGCTAATCTGGATCAAATACTGCATTAATTATGTAAACACTCAATAAAGTTTAATCCTTTTGTTCATATTCTTAATCCAGATCAAAGAAGAAGAGAAAAAGATTGAAAATTCATCACAATTACCAAGTGGGTTATTAAAATCCCACAGATTAACATCAATGATGAACAGCAAATCAAGAGTTTTAGCCAACAGGAACCCATCACTCCCCCCACGCCCAAAGACAGCAGCAGCAGCAGCTCAGCCAATAACCCCCTGCGCCGCCCCGCCTACCGCAGCGTCGTTGAATGCCTACGAGTACTACTGGAGGTCGAAGCCGAGCTCGAAAGCGGGGGCAGCGGCTGTGAACCCGCTCGGGCAGCAGGCGGCAGCGTTCAAGAATAGCAACAGGAAGATTATGATATGCAAGAACAGAGCAGTGTATGAAGAAGAGCAAATGTTGGTGATGTCTCCAAAAGGGAGCTGTAAGGAGGTCCGGCGGACGGCGGTGGTTTGGGAGCCACGGTGCATTGCCACATCCTAAGTAGTTGATGATATTCATTTTGGCGAAGGCTGTGTAAGCTAACTGTTGTAGCTGGGTGTGAATTGGGTTCTTAATTTAGTGTTAACTTACCTTTCCACTCTTTGGGGAATTGTGTTGAAAAGAATAAAAAGGTGTTGAAAGAGAAAGAGTGAGAGATTATTACTTTTTATTTGAGAGAAATATTTACCCTTCATGAAGTTTAATAATATATATATAGTTAAATTATTAATTT

mRNA sequence

GGGCCCTCTTCTCGATTCCCATGGCGGACTATAAATACCCACTTTCCTCTTCCCCTTTCGTGGCAACCTCATTGCATCATCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCACTTCCCCTGCTTCCCTTCTTTGCAACTCTGCACCTTCAGCGGTCTCCTTCTTCCGACCGCTGCCGGTTGCTGTAGTTGCTCTTCTAGAAACACTCAAAAAAACCCAACAACACAAAACCAAATCATGTCGCCATTGAAGATTTTTCAAGCTTCCTGCGACGACAAAAAACAGGGCATCGTCGCTGTTCTGTCCTCCGATCCCGACCGCTCCAAATCCCAACCTTCTTCTCTCCGAAGAACACTCTCTGCTGACATGTCGTCCCATAAATGGATTTCCCAAATGAAAAATGTCTCTTCTTGTCAAGAATTTTGTCTCTGGGATTCAATTCAAGAAAGGGAGAAAAAGAAGAAGAAAACAGAGGAGGATAAAACAGAGTCTCCTGATATTTGGGGCTCTGTCATCAAATCTCGCAAGGATTTTAATGCTAATCCTTATGTTCCTCCCCGGAAAATGGGTAAGTGTTTAAGTGAGAAAAGCCTCGAGATTTGTACTGAAAGTCTTGGATCGGAAACCGGCTCTGATGGGTTCTCGTCGTATCCATCATCGGAGAACGGAGATTCCGACGAGGAGGAGTTTGAAGTAGAGGATCAAGGGAAGCCGGTGCAGTATAGCTATAAAAAATCACCCCCCCGCCGGTCGTTTCCTCCCCCGTTGCCGACACTCTCCGGCCCGGATGGGGAGTTAGTACGGATGCAGCCCCGCCGTGACAACGGCCGGTTGGTCCTCGAGGCCATGTCCGTGCCTTCACAGAGCACCTTCTCCGCCCAACGTCAAGATGGCCGCCTCGTTCTCACCCTCGTTAATCGCAAGCCCATTAACGAGATCAAAGAAGAAGAGAAAAAGATTGAAAATTCATCACAATTACCAAGTGGGTTATTAAAATCCCACAGATTAACATCAATGATGAACAGCAAATCAAGAGTTTTAGCCAACAGGAACCCATCACTCCCCCCACGCCCAAAGACAGCAGCAGCAGCAGCTCAGCCAATAACCCCCTGCGCCGCCCCGCCTACCGCAGCGTCGTTGAATGCCTACGAGTACTACTGGAGGTCGAAGCCGAGCTCGAAAGCGGGGGCAGCGGCTGTGAACCCGCTCGGGCAGCAGGCGGCAGCGTTCAAGAATAGCAACAGGAAGATTATGATATGCAAGAACAGAGCAGTGTATGAAGAAGAGCAAATGTTGGTGATGTCTCCAAAAGGGAGCTGTAAGGAGGTCCGGCGGACGGCGGTGGTTTGGGAGCCACGGTGCATTGCCACATCCTAAGTAGTTGATGATATTCATTTTGGCGAAGGCTGTGTAAGCTAACTGTTGTAGCTGGGTGTGAATTGGGTTCTTAATTTAGTGTTAACTTACCTTTCCACTCTTTGGGGAATTGTGTTGAAAAGAATAAAAAGGTGTTGAAAGAGAAAGAGTGAGAGATTATTACTTTTTATTTGAGAGAAATATTTACCCTTCATGAAGTTTAATAATATATATATAGTTAAATTATTAATTT

Coding sequence (CDS)

ATGGCGGACTATAAATACCCACTTTCCTCTTCCCCTTTCGTGGCAACCTCATTGCATCATCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCACTTCCCCTGCTTCCCTTCTTTGCAACTCTGCACCTTCAGCGGTCTCCTTCTTCCGACCGCTGCCGGTTGCTGTAGTTGCTCTTCTAGAAACACTCAAAAAAACCCAACAACACAAAACCAAATCATGTCGCCATTGAAGATTTTTCAAGCTTCCTGCGACGACAAAAAACAGGGCATCGTCGCTGTTCTGTCCTCCGATCCCGACCGCTCCAAATCCCAACCTTCTTCTCTCCGAAGAACACTCTCTGCTGACATGTCGTCCCATAAATGGATTTCCCAAATGAAAAATGTCTCTTCTTGTCAAGAATTTTGTCTCTGGGATTCAATTCAAGAAAGGGAGAAAAAGAAGAAGAAAACAGAGGAGGATAAAACAGAGTCTCCTGATATTTGGGGCTCTGTCATCAAATCTCGCAAGGATTTTAATGCTAATCCTTATGTTCCTCCCCGGAAAATGGGTAAGTGTTTAAGTGAGAAAAGCCTCGAGATTTGTACTGAAAGTCTTGGATCGGAAACCGGCTCTGATGGGTTCTCGTCGTATCCATCATCGGAGAACGGAGATTCCGACGAGGAGGAGTTTGAAGTAGAGGATCAAGGGAAGCCGGTGCAGTATAGCTATAAAAAATCACCCCCCCGCCGGTCGTTTCCTCCCCCGTTGCCGACACTCTCCGGCCCGGATGGGGAGTTAGTACGGATGCAGCCCCGCCGTGACAACGGCCGGTTGGTCCTCGAGGCCATGTCCGTGCCTTCACAGAGCACCTTCTCCGCCCAACGTCAAGATGGCCGCCTCGTTCTCACCCTCGTTAATCGCAAGCCCATTAACGAGATCAAAGAAGAAGAGAAAAAGATTGAAAATTCATCACAATTACCAAGTGGGTTATTAAAATCCCACAGATTAACATCAATGATGAACAGCAAATCAAGAGTTTTAGCCAACAGGAACCCATCACTCCCCCCACGCCCAAAGACAGCAGCAGCAGCAGCTCAGCCAATAACCCCCTGCGCCGCCCCGCCTACCGCAGCGTCGTTGAATGCCTACGAGTACTACTGGAGGTCGAAGCCGAGCTCGAAAGCGGGGGCAGCGGCTGTGAACCCGCTCGGGCAGCAGGCGGCAGCGTTCAAGAATAGCAACAGGAAGATTATGATATGCAAGAACAGAGCAGTGTATGAAGAAGAGCAAATGTTGGTGATGTCTCCAAAAGGGAGCTGTAAGGAGGTCCGGCGGACGGCGGTGGTTTGGGAGCCACGGTGCATTGCCACATCCTAA

Protein sequence

MADYKYPLSSSPFVATSLHHLLLLLLLLLLLHFPCFPSLQLCTFSGLLLPTAAGCCSCSSRNTQKNPTTQNQIMSPLKIFQASCDDKKQGIVAVLSSDPDRSKSQPSSLRRTLSADMSSHKWISQMKNVSSCQEFCLWDSIQEREKKKKKTEEDKTESPDIWGSVIKSRKDFNANPYVPPRKMGKCLSEKSLEICTESLGSETGSDGFSSYPSSENGDSDEEEFEVEDQGKPVQYSYKKSPPRRSFPPPLPTLSGPDGELVRMQPRRDNGRLVLEAMSVPSQSTFSAQRQDGRLVLTLVNRKPINEIKEEEKKIENSSQLPSGLLKSHRLTSMMNSKSRVLANRNPSLPPRPKTAAAAAQPITPCAAPPTAASLNAYEYYWRSKPSSKAGAAAVNPLGQQAAAFKNSNRKIMICKNRAVYEEEQMLVMSPKGSCKEVRRTAVVWEPRCIATS
Homology
BLAST of Cp4.1LG04g12540.1 vs. ExPASy Swiss-Prot
Match: Q0V865 (Protein FAF-like, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g22090 PE=2 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 3.7e-39
Identity = 157/466 (33.69%), Postives = 212/466 (45.49%), Query Frame = 0

Query: 87  KKQGIVAVLSSDPDRSKSQPSSLRRTLSADMSSHKWISQ-----MKNVSSCQEF------ 146
           K+QGIV++L      + S P SLRRT SAD+SS  W+SQ     MK +SS ++       
Sbjct: 18  KQQGIVSILGGISSNTSSAP-SLRRTFSADLSSKTWVSQNGFSPMKRISSSEKLRPDEEE 77

Query: 147 ---------CLWDSIQEREKKKKKTEEDKTESPDIWGSVI------KSRKDFNANPYVPP 206
                     +W  IQ+ +  KKK EE +    D+W S++      +S KD    PYV P
Sbjct: 78  AEEESRSGVDIWAQIQQDKNDKKKEEEIEPGQSDVWSSILSEKKKTESSKDTVPPPYVHP 137

Query: 207 -RKMGKCLSEKSLEICTESLGSETGSDGFSSYPSSENGDSD------------------- 266
             K    LSEKSLEICTESLGSETG DGFSS+ SSE GD++                   
Sbjct: 138 LMKRASSLSEKSLEICTESLGSETGCDGFSSHASSETGDAEIEIHDETNLVVNVTETKVE 197

Query: 267 ---EEEFEVEDQGKPVQYSYKKSPPRRSFPPPLPTLSGPDGELVRMQPRRDNGRLVLEAM 326
              E E  VE +   +  ++    P  SFPPP+ +LS   G  + M+ RRDNGRLVLEA+
Sbjct: 198 EITETEIVVEQESSIIVPNHIIELPPGSFPPPIRSLSSQSGSSLHMKTRRDNGRLVLEAV 257

Query: 327 SVPSQSTFSAQRQDGRLVLTL--VNRKP--------------INEIKEEEKKIENSSQ-- 386
           S+PS + FSA+RQDGRL+LT   ++ +P               +E +EEE++ E+  +  
Sbjct: 258 SMPSHNNFSAKRQDGRLLLTFAEISNEPNYDKEDEIDSEVQWFDEEEEEEEEEEDEEEEA 317

Query: 387 ------LPSGLLKS----------HRL----------------TSMMNSKSRVLANRNPS 446
                  P+GLL            HRL                    ++KS +      S
Sbjct: 318 PDEFAYKPNGLLYKMAQKPIPITVHRLAHKPIGVPKRNSRWPMADEFDTKSDLSTPVVHS 377

Query: 447 LPPRPKTAAAAAQPITPCAAPPT--AASLNAYEYYWRSKPSSKAGAAAVNPLGQQAAAFK 452
           LPPRP+ A  A     P     T  AA  N  +Y W+S  +   G         QA  F 
Sbjct: 378 LPPRPRVAQLARSTKPPSTVDDTVGAACFNTCDYSWKSTNTESFGPNTKTQF--QAQNFV 437

BLAST of Cp4.1LG04g12540.1 vs. ExPASy Swiss-Prot
Match: Q6NMR8 (Protein FANTASTIC FOUR 3 OS=Arabidopsis thaliana OX=3702 GN=FAF3 PE=2 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 9.9e-08
Identity = 70/233 (30.04%), Postives = 111/233 (47.64%), Query Frame = 0

Query: 97  SDPDRSKSQPSSLRRTLSADMSSHKWISQMKN-----VSSCQEFCLWDSIQEREKKKKKT 156
           S P+   SQP  L        SSH   ++ +N      S   +   W S+Q        +
Sbjct: 25  SSPNPHFSQPFGLALKSHLLDSSHAEDTRNRNDDKAAASPVSDSSGWSSLQSLSSGSSSS 84

Query: 157 EEDKTESPDIWGSVIKSRKDFNANPYVPPRKMGKCLSEKSLEICTESLGSETGSDG---- 216
            +  T S            +  ++ YV      + LS++SL +CTE+LGSE+GSD     
Sbjct: 85  TKTTTSS------------EKESSYYVQRPSSCRALSDQSLALCTENLGSESGSDVTDID 144

Query: 217 --FSSYPSSEN-GDSDEEEFEVEDQGKPVQYSYKKSPPRRSFPPPLPTLSGPDGELVRMQ 276
             FS    ++N G++  E   ++        S K+S      PPPL T+ G   + ++M+
Sbjct: 145 ELFSLDVQTKNLGETTTETRTLK--------SRKRSVSPSDLPPPLTTMRG--FQCIQMR 204

Query: 277 PRRDNGRLVLEAMSVPSQS-TFSAQRQDGRLVLTLVNRKPINE-IKEEEKKIE 316
           P R+NGRLV+ A + P ++  F A R +GRL L+++  K  NE ++ EE+ IE
Sbjct: 205 PHRENGRLVMTATNAPPRNGCFQADRSNGRLRLSIL--KDSNEFVENEEETIE 233

BLAST of Cp4.1LG04g12540.1 vs. ExPASy Swiss-Prot
Match: Q9SFG6 (Protein FANTASTIC FOUR 4 OS=Arabidopsis thaliana OX=3702 GN=FAF4 PE=2 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 4.6e-05
Identity = 43/138 (31.16%), Postives = 66/138 (47.83%), Query Frame = 0

Query: 185 KCLSEKSLEICTESLGSETGSDGFSSYPSSENGDSDEEEFEVEDQGKPVQYSYKKSPPRR 244
           + LS++SL +CTESLGSETGSD       S + +    E              +K     
Sbjct: 106 RTLSDESLALCTESLGSETGSDIIHEDMFSISSELQTMETRTTSTTSNPSRQDRKRNTMA 165

Query: 245 SFPPPLPTLSGPDGELVRMQPRRDNGRLVLEAMSVPSQSTFSAQRQDG--RLVLTLVNRK 304
           S PPPL ++ G D   + ++  R+NGRLV+ A   P ++     R +G  RL + + +  
Sbjct: 166 SLPPPLTSMIGFD--CIEVKSHRENGRLVMMATRPPPRNRCLQDRSNGCVRLAILIDSDD 225

Query: 305 PINEIKEEEKKIENSSQL 321
            I    +EEK+ E    +
Sbjct: 226 HIETETKEEKEEEEEETI 241

BLAST of Cp4.1LG04g12540.1 vs. ExPASy Swiss-Prot
Match: Q8GXU9 (Protein FANTASTIC FOUR 2 OS=Arabidopsis thaliana OX=3702 GN=FAF2 PE=2 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 7.8e-05
Identity = 56/169 (33.14%), Postives = 92/169 (54.44%), Query Frame = 0

Query: 164 SVIKSRKDFNANPYVPP---RKMGKCLSEKSLEICTESLGSETGSD-----GFSSYPSSE 223
           S+ ++++D     YV P   R + K L+EKSLE+CTESLG+ETGS+        ++ ++ 
Sbjct: 34  SIAQTKED---KAYVHPMEKRSVSK-LNEKSLEMCTESLGTETGSESGDELSLLAFEATT 93

Query: 224 NGDSDEEEFE-VEDQGKPVQYSYKKSPP---RRSFPPPLPTLSGPDGELVRM-QPRRDNG 283
              +   + +  ED   P      K+PP     SFPPP+  +   D +  RM +   ++G
Sbjct: 94  TPRAPPRQLKPQEDTNLP-----DKTPPMSRNNSFPPPIKFVE--DSKYNRMVRWLGEDG 153

Query: 284 RLVLEAMSVPS-QSTFSAQRQDGRLVLTLVNRKPI---NEIKEEEKKIE 316
           R+V++A+ V S  S F ++R +GRL L L +   +   N  +EEE++ E
Sbjct: 154 RIVVQAIRVSSPPSCFVSERGEGRLRLILTSESSLLSHNHEEEEEEETE 191

BLAST of Cp4.1LG04g12540.1 vs. ExPASy Swiss-Prot
Match: Q9SY06 (Protein FANTASTIC FOUR 1 OS=Arabidopsis thaliana OX=3702 GN=FAF1 PE=2 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 6.6e-04
Identity = 50/177 (28.25%), Postives = 92/177 (51.98%), Query Frame = 0

Query: 156 TESPDIWG-----------SVIKSRK-DFNANPYVPPRKMGKCLSEKSLEICTESLGSET 215
           +++PD+ G            ++++R+ D     YV P +  + +++ SLE+CTESLG+E 
Sbjct: 17  SQNPDVGGWSFLQYFSEPKGIVQNREDDTKKTAYVYPIEK-RSVAKLSLEMCTESLGTEN 76

Query: 216 GSDG---FSSYPSSENGDSDEEEFEVEDQGKPVQYSYKKSPPRRSFPPPLPTLSGPDGEL 275
           GSD     S         S       + Q K   +  +++    SFPPPL +++G +   
Sbjct: 77  GSDSGDEMSLLALEATNISKSPRLTTKPQ-KETSFMTREN----SFPPPLNSVNGFNNSR 136

Query: 276 VRMQPRRDNGRLVLEAMSVPS-QSTFSAQRQDGRLVLTLVNRKPINEIKEEEKKIEN 317
           + ++  +++GRLV++A+ V S    F ++R++GRL L L      ++  EEE + E+
Sbjct: 137 M-VKSYKEDGRLVVQAIRVCSPPRCFVSERREGRLRLCLSQNSLNSQDAEEEFEEED 186

BLAST of Cp4.1LG04g12540.1 vs. NCBI nr
Match: KAG6588246.1 (Protein FAF-like, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 854 bits (2206), Expect = 1.72e-311
Identity = 441/452 (97.57%), Postives = 446/452 (98.67%), Query Frame = 0

Query: 1   MADYKYPLSSSPFVATSLHHLLLLLLLLLLLHFPCFPSLQLCTFSGLLLPTAAGCCSCSS 60
           MADYKYPLSSSPFVATSLHH LLLLLLLLLLHFPCFPSLQLCTFSGLLLPTAAGCCSCSS
Sbjct: 13  MADYKYPLSSSPFVATSLHHHLLLLLLLLLLHFPCFPSLQLCTFSGLLLPTAAGCCSCSS 72

Query: 61  RNTQKNPTTQNQIMSPLKIFQASCDDKKQGIVAVLSSDPDRSKSQPSSLRRTLSADMSSH 120
           RNTQKNP TQNQIMSPLKIFQASCDDKKQGIVAVLSSDPDRSKSQPSSLRRTLSADMSSH
Sbjct: 73  RNTQKNPITQNQIMSPLKIFQASCDDKKQGIVAVLSSDPDRSKSQPSSLRRTLSADMSSH 132

Query: 121 KWISQMKNVSSCQEFCLWDSIQEREKKKKKTEEDKTESPDIWGSVIKSRKDFNANPYVPP 180
           KWISQMKNV+SCQEF LWDSIQEREKKKKKTEEDKTES DIWGSVIKSRKDFNANPYVPP
Sbjct: 133 KWISQMKNVASCQEFGLWDSIQEREKKKKKTEEDKTESADIWGSVIKSRKDFNANPYVPP 192

Query: 181 RKMGKCLSEKSLEICTESLGSETGSDGFSSYPSSENGDSDEEEFEVEDQGKPVQYSYKKS 240
           R+MGKCLSEKSLEICTESLGSETGSDGFSSYPSSENGDSDEEEFEVEDQGKP++YSYKKS
Sbjct: 193 RQMGKCLSEKSLEICTESLGSETGSDGFSSYPSSENGDSDEEEFEVEDQGKPMKYSYKKS 252

Query: 241 PPRRSFPPPLPTLSGPDGELVRMQPRRDNGRLVLEAMSVPSQSTFSAQRQDGRLVLTLVN 300
           PPRRSFPPPLPTLSGPDGELVRMQPRRDNGRLVLEAMSVPSQSTFSAQRQDGRLVLTLVN
Sbjct: 253 PPRRSFPPPLPTLSGPDGELVRMQPRRDNGRLVLEAMSVPSQSTFSAQRQDGRLVLTLVN 312

Query: 301 RKPINEIKEEEKKIENSSQLPSGLLKSHRLTSMMNSKSRVLANRNPSLPPRPKTAAAAAQ 360
           RKPINEIKEEEK+IENSSQLPSGLLKSHRL SMMNSKSRVLANRNPSLPPRPKTAAAAAQ
Sbjct: 313 RKPINEIKEEEKEIENSSQLPSGLLKSHRLASMMNSKSRVLANRNPSLPPRPKTAAAAAQ 372

Query: 361 PITPCAAPPTAASLNAYEYYWRSKPSSKAGAAAVNPLGQQAAAFKNSNRKIMICKNRAVY 420
           PITPCAAPPTAASLNAYEYYWRSKPSSKAGAAAVNPLGQQAAAFKNSNRKIMICKNRAV 
Sbjct: 373 PITPCAAPPTAASLNAYEYYWRSKPSSKAGAAAVNPLGQQAAAFKNSNRKIMICKNRAVN 432

Query: 421 EEEQMLVMSPKGSCKEVRRTAVVWEPRCIATS 452
           EEEQMLVMSPKGSCKEVRRTAVVWEPRCIATS
Sbjct: 433 EEEQMLVMSPKGSCKEVRRTAVVWEPRCIATS 464

BLAST of Cp4.1LG04g12540.1 vs. NCBI nr
Match: KAG7022163.1 (Protein FAF-like, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 846 bits (2185), Expect = 1.80e-308
Identity = 440/453 (97.13%), Postives = 445/453 (98.23%), Query Frame = 0

Query: 1   MADYKYPLSSSPFVATSLHH-LLLLLLLLLLLHFPCFPSLQLCTFSGLLLPTAAGCCSCS 60
           MADYKYPLSSSPFVATSLHH LLLLLLLLLLLHFPCFPSLQLCTFSGLLLPTAAGCCSCS
Sbjct: 1   MADYKYPLSSSPFVATSLHHHLLLLLLLLLLLHFPCFPSLQLCTFSGLLLPTAAGCCSCS 60

Query: 61  SRNTQKNPTTQNQIMSPLKIFQASCDDKKQGIVAVLSSDPDRSKSQPSSLRRTLSADMSS 120
           SRNT+KNP TQNQIMS LKIFQASCDDKKQGIVAVLSSDPDRSKSQPSSLRRTLSADMSS
Sbjct: 61  SRNTKKNPITQNQIMSALKIFQASCDDKKQGIVAVLSSDPDRSKSQPSSLRRTLSADMSS 120

Query: 121 HKWISQMKNVSSCQEFCLWDSIQEREKKKKKTEEDKTESPDIWGSVIKSRKDFNANPYVP 180
           HKWISQMKNV+SCQEF LWDSIQEREKKKKKTEEDKTES DIWGSVIKS KDFNANPYVP
Sbjct: 121 HKWISQMKNVASCQEFGLWDSIQEREKKKKKTEEDKTESADIWGSVIKSCKDFNANPYVP 180

Query: 181 PRKMGKCLSEKSLEICTESLGSETGSDGFSSYPSSENGDSDEEEFEVEDQGKPVQYSYKK 240
           PR+MGKCLSEKSLEICTESLGSETGSDGFSSYPSSENGDSDEEEFEVEDQGKP++YSYKK
Sbjct: 181 PRQMGKCLSEKSLEICTESLGSETGSDGFSSYPSSENGDSDEEEFEVEDQGKPMKYSYKK 240

Query: 241 SPPRRSFPPPLPTLSGPDGELVRMQPRRDNGRLVLEAMSVPSQSTFSAQRQDGRLVLTLV 300
           SPPRRSFPPPLPTLSGPDGELVRMQPRRDNGRLVLEAMSVPSQSTFSAQRQDGRLVLTLV
Sbjct: 241 SPPRRSFPPPLPTLSGPDGELVRMQPRRDNGRLVLEAMSVPSQSTFSAQRQDGRLVLTLV 300

Query: 301 NRKPINEIKEEEKKIENSSQLPSGLLKSHRLTSMMNSKSRVLANRNPSLPPRPKTAAAAA 360
           NRKPINEIKEEEKKIENSSQLPSGLLKSHRL SMMNSKSRVLANRNPSLPPRPKTAAAAA
Sbjct: 301 NRKPINEIKEEEKKIENSSQLPSGLLKSHRLASMMNSKSRVLANRNPSLPPRPKTAAAAA 360

Query: 361 QPITPCAAPPTAASLNAYEYYWRSKPSSKAGAAAVNPLGQQAAAFKNSNRKIMICKNRAV 420
           QPITPCAAPPTAASLNAYEYYWRSKPSSKAGAAAVNPLGQQAAAFKNSNRKIMICKNRAV
Sbjct: 361 QPITPCAAPPTAASLNAYEYYWRSKPSSKAGAAAVNPLGQQAAAFKNSNRKIMICKNRAV 420

Query: 421 YEEEQMLVMSPKGSCKEVRRTAVVWEPRCIATS 452
            EEEQMLVMSPKGSCKEVRRTAVVWEPRCIATS
Sbjct: 421 NEEEQMLVMSPKGSCKEVRRTAVVWEPRCIATS 453

BLAST of Cp4.1LG04g12540.1 vs. NCBI nr
Match: XP_023529951.1 (protein FAF-like, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 737 bits (1903), Expect = 1.01e-266
Identity = 379/379 (100.00%), Postives = 379/379 (100.00%), Query Frame = 0

Query: 74  MSPLKIFQASCDDKKQGIVAVLSSDPDRSKSQPSSLRRTLSADMSSHKWISQMKNVSSCQ 133
           MSPLKIFQASCDDKKQGIVAVLSSDPDRSKSQPSSLRRTLSADMSSHKWISQMKNVSSCQ
Sbjct: 1   MSPLKIFQASCDDKKQGIVAVLSSDPDRSKSQPSSLRRTLSADMSSHKWISQMKNVSSCQ 60

Query: 134 EFCLWDSIQEREKKKKKTEEDKTESPDIWGSVIKSRKDFNANPYVPPRKMGKCLSEKSLE 193
           EFCLWDSIQEREKKKKKTEEDKTESPDIWGSVIKSRKDFNANPYVPPRKMGKCLSEKSLE
Sbjct: 61  EFCLWDSIQEREKKKKKTEEDKTESPDIWGSVIKSRKDFNANPYVPPRKMGKCLSEKSLE 120

Query: 194 ICTESLGSETGSDGFSSYPSSENGDSDEEEFEVEDQGKPVQYSYKKSPPRRSFPPPLPTL 253
           ICTESLGSETGSDGFSSYPSSENGDSDEEEFEVEDQGKPVQYSYKKSPPRRSFPPPLPTL
Sbjct: 121 ICTESLGSETGSDGFSSYPSSENGDSDEEEFEVEDQGKPVQYSYKKSPPRRSFPPPLPTL 180

Query: 254 SGPDGELVRMQPRRDNGRLVLEAMSVPSQSTFSAQRQDGRLVLTLVNRKPINEIKEEEKK 313
           SGPDGELVRMQPRRDNGRLVLEAMSVPSQSTFSAQRQDGRLVLTLVNRKPINEIKEEEKK
Sbjct: 181 SGPDGELVRMQPRRDNGRLVLEAMSVPSQSTFSAQRQDGRLVLTLVNRKPINEIKEEEKK 240

Query: 314 IENSSQLPSGLLKSHRLTSMMNSKSRVLANRNPSLPPRPKTAAAAAQPITPCAAPPTAAS 373
           IENSSQLPSGLLKSHRLTSMMNSKSRVLANRNPSLPPRPKTAAAAAQPITPCAAPPTAAS
Sbjct: 241 IENSSQLPSGLLKSHRLTSMMNSKSRVLANRNPSLPPRPKTAAAAAQPITPCAAPPTAAS 300

Query: 374 LNAYEYYWRSKPSSKAGAAAVNPLGQQAAAFKNSNRKIMICKNRAVYEEEQMLVMSPKGS 433
           LNAYEYYWRSKPSSKAGAAAVNPLGQQAAAFKNSNRKIMICKNRAVYEEEQMLVMSPKGS
Sbjct: 301 LNAYEYYWRSKPSSKAGAAAVNPLGQQAAAFKNSNRKIMICKNRAVYEEEQMLVMSPKGS 360

Query: 434 CKEVRRTAVVWEPRCIATS 452
           CKEVRRTAVVWEPRCIATS
Sbjct: 361 CKEVRRTAVVWEPRCIATS 379

BLAST of Cp4.1LG04g12540.1 vs. NCBI nr
Match: XP_022924675.1 (protein FAF-like, chloroplastic [Cucurbita moschata])

HSP 1 Score: 713 bits (1840), Expect = 4.01e-257
Identity = 368/379 (97.10%), Postives = 372/379 (98.15%), Query Frame = 0

Query: 74  MSPLKIFQASCDDKKQGIVAVLSSDPDRSKSQPSSLRRTLSADMSSHKWISQMKNVSSCQ 133
           MSPLKIFQASCDDKKQGIVAVLSSDPDRSKSQPSSLRRTLSADMSSHKWISQMKNV+SCQ
Sbjct: 1   MSPLKIFQASCDDKKQGIVAVLSSDPDRSKSQPSSLRRTLSADMSSHKWISQMKNVASCQ 60

Query: 134 EFCLWDSIQEREKKKKKTEEDKTESPDIWGSVIKSRKDFNANPYVPPRKMGKCLSEKSLE 193
           EF LWDSIQEREKKKKKTEEDKTES DIWGSVIKSRKDFNANPYVPPR+MGKCLSEKSLE
Sbjct: 61  EFGLWDSIQEREKKKKKTEEDKTESADIWGSVIKSRKDFNANPYVPPRQMGKCLSEKSLE 120

Query: 194 ICTESLGSETGSDGFSSYPSSENGDSDEEEFEVEDQGKPVQYSYKKSPPRRSFPPPLPTL 253
           ICTESLGSETGSDGFSSYPSSENGDSDEEEFEVEDQGKP++YSYKKSPPRRSFPPPLPTL
Sbjct: 121 ICTESLGSETGSDGFSSYPSSENGDSDEEEFEVEDQGKPMKYSYKKSPPRRSFPPPLPTL 180

Query: 254 SGPDGELVRMQPRRDNGRLVLEAMSVPSQSTFSAQRQDGRLVLTLVNRKPINEIKEEEKK 313
           SGPDGELVRMQPRRDNGRLVLEAMSVPSQSTFSAQRQDGRLVLTLVNRKPINEIKEEEKK
Sbjct: 181 SGPDGELVRMQPRRDNGRLVLEAMSVPSQSTFSAQRQDGRLVLTLVNRKPINEIKEEEKK 240

Query: 314 IENSSQLPSGLLKSHRLTSMMNSKSRVLANRNPSLPPRPKTAAAAAQPITPCAAPPTAAS 373
           IENSSQLPSGLLKSHRL SMMN KSRVLANRNPSLPPRPKTAAAAAQPITPCAAPPTAA 
Sbjct: 241 IENSSQLPSGLLKSHRLASMMNRKSRVLANRNPSLPPRPKTAAAAAQPITPCAAPPTAAL 300

Query: 374 LNAYEYYWRSKPSSKAGAAAVNPLGQQAAAFKNSNRKIMICKNRAVYEEEQMLVMSPKGS 433
           LNAYEYYWRSKPSSKAGAAAVNPLGQQ AAFKNSNRKIMICKNRAV EEEQMLVMSPKGS
Sbjct: 301 LNAYEYYWRSKPSSKAGAAAVNPLGQQGAAFKNSNRKIMICKNRAVNEEEQMLVMSPKGS 360

Query: 434 CKEVRRTAVVWEPRCIATS 452
           CKEVRRTAVVWEPRCIATS
Sbjct: 361 CKEVRRTAVVWEPRCIATS 379

BLAST of Cp4.1LG04g12540.1 vs. NCBI nr
Match: XP_022972071.1 (protein FAF-like, chloroplastic [Cucurbita maxima])

HSP 1 Score: 707 bits (1824), Expect = 1.27e-254
Identity = 367/383 (95.82%), Postives = 371/383 (96.87%), Query Frame = 0

Query: 74  MSPLKIFQASCDDKKQGIVAVLSSDPDRSKSQPSSLRRTLSADMSSHKWISQMKNVSSCQ 133
           MSPLKIFQASCDDKKQGIVAVLSSDPDRSKSQPSSLRRTLSADMSSHKWISQMKNV+SCQ
Sbjct: 1   MSPLKIFQASCDDKKQGIVAVLSSDPDRSKSQPSSLRRTLSADMSSHKWISQMKNVASCQ 60

Query: 134 EFCLWDSIQEREKKKKKTEEDKTE----SPDIWGSVIKSRKDFNANPYVPPRKMGKCLSE 193
           EF LWDSIQEREKKKKKTEEDKTE    S DIWGSVIKSRKDFNANPYVPPR MGKCLSE
Sbjct: 61  EFGLWDSIQEREKKKKKTEEDKTEDKTDSADIWGSVIKSRKDFNANPYVPPRPMGKCLSE 120

Query: 194 KSLEICTESLGSETGSDGFSSYPSSENGDSDEEEFEVEDQGKPVQYSYKKSPPRRSFPPP 253
           KSLEICTESLGSETGSDGFSSYPSSENGDSDEEEFEVEDQGKPV+YSYKKSPPRRSFPPP
Sbjct: 121 KSLEICTESLGSETGSDGFSSYPSSENGDSDEEEFEVEDQGKPVKYSYKKSPPRRSFPPP 180

Query: 254 LPTLSGPDGELVRMQPRRDNGRLVLEAMSVPSQSTFSAQRQDGRLVLTLVNRKPINEIKE 313
           LPTLSGPDGELVRMQPRRDNGRLVLEAMSVPSQSTFSAQRQDGRLVLTLVNRKPINEIKE
Sbjct: 181 LPTLSGPDGELVRMQPRRDNGRLVLEAMSVPSQSTFSAQRQDGRLVLTLVNRKPINEIKE 240

Query: 314 EEKKIENSSQLPSGLLKSHRLTSMMNSKSRVLANRNPSLPPRPKTAAAAAQPITPCAAPP 373
           E+KKIENSSQLPSGLLKSHRL SMMNSKSRVLANRNPSLPPRPK AAAAAQPITPCAAPP
Sbjct: 241 EQKKIENSSQLPSGLLKSHRLASMMNSKSRVLANRNPSLPPRPKAAAAAAQPITPCAAPP 300

Query: 374 TAASLNAYEYYWRSKPSSKAGAAAVNPLGQQAAAFKNSNRKIMICKNRAVYEEEQMLVMS 433
           TAASLNAYEYYWRSKPSSKAGAAAVNPLGQQAAAFKNSNRK+MICKNR V EEEQM VMS
Sbjct: 301 TAASLNAYEYYWRSKPSSKAGAAAVNPLGQQAAAFKNSNRKVMICKNRVVNEEEQMFVMS 360

Query: 434 PKGSCKEVRRTAVVWEPRCIATS 452
           PKGSCKEVRRTAVVWEPRCIATS
Sbjct: 361 PKGSCKEVRRTAVVWEPRCIATS 383

BLAST of Cp4.1LG04g12540.1 vs. ExPASy TrEMBL
Match: A0A6J1EFP6 (protein FAF-like, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111432104 PE=3 SV=1)

HSP 1 Score: 713 bits (1840), Expect = 1.94e-257
Identity = 368/379 (97.10%), Postives = 372/379 (98.15%), Query Frame = 0

Query: 74  MSPLKIFQASCDDKKQGIVAVLSSDPDRSKSQPSSLRRTLSADMSSHKWISQMKNVSSCQ 133
           MSPLKIFQASCDDKKQGIVAVLSSDPDRSKSQPSSLRRTLSADMSSHKWISQMKNV+SCQ
Sbjct: 1   MSPLKIFQASCDDKKQGIVAVLSSDPDRSKSQPSSLRRTLSADMSSHKWISQMKNVASCQ 60

Query: 134 EFCLWDSIQEREKKKKKTEEDKTESPDIWGSVIKSRKDFNANPYVPPRKMGKCLSEKSLE 193
           EF LWDSIQEREKKKKKTEEDKTES DIWGSVIKSRKDFNANPYVPPR+MGKCLSEKSLE
Sbjct: 61  EFGLWDSIQEREKKKKKTEEDKTESADIWGSVIKSRKDFNANPYVPPRQMGKCLSEKSLE 120

Query: 194 ICTESLGSETGSDGFSSYPSSENGDSDEEEFEVEDQGKPVQYSYKKSPPRRSFPPPLPTL 253
           ICTESLGSETGSDGFSSYPSSENGDSDEEEFEVEDQGKP++YSYKKSPPRRSFPPPLPTL
Sbjct: 121 ICTESLGSETGSDGFSSYPSSENGDSDEEEFEVEDQGKPMKYSYKKSPPRRSFPPPLPTL 180

Query: 254 SGPDGELVRMQPRRDNGRLVLEAMSVPSQSTFSAQRQDGRLVLTLVNRKPINEIKEEEKK 313
           SGPDGELVRMQPRRDNGRLVLEAMSVPSQSTFSAQRQDGRLVLTLVNRKPINEIKEEEKK
Sbjct: 181 SGPDGELVRMQPRRDNGRLVLEAMSVPSQSTFSAQRQDGRLVLTLVNRKPINEIKEEEKK 240

Query: 314 IENSSQLPSGLLKSHRLTSMMNSKSRVLANRNPSLPPRPKTAAAAAQPITPCAAPPTAAS 373
           IENSSQLPSGLLKSHRL SMMN KSRVLANRNPSLPPRPKTAAAAAQPITPCAAPPTAA 
Sbjct: 241 IENSSQLPSGLLKSHRLASMMNRKSRVLANRNPSLPPRPKTAAAAAQPITPCAAPPTAAL 300

Query: 374 LNAYEYYWRSKPSSKAGAAAVNPLGQQAAAFKNSNRKIMICKNRAVYEEEQMLVMSPKGS 433
           LNAYEYYWRSKPSSKAGAAAVNPLGQQ AAFKNSNRKIMICKNRAV EEEQMLVMSPKGS
Sbjct: 301 LNAYEYYWRSKPSSKAGAAAVNPLGQQGAAFKNSNRKIMICKNRAVNEEEQMLVMSPKGS 360

Query: 434 CKEVRRTAVVWEPRCIATS 452
           CKEVRRTAVVWEPRCIATS
Sbjct: 361 CKEVRRTAVVWEPRCIATS 379

BLAST of Cp4.1LG04g12540.1 vs. ExPASy TrEMBL
Match: A0A6J1I4Y7 (protein FAF-like, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111470708 PE=3 SV=1)

HSP 1 Score: 707 bits (1824), Expect = 6.17e-255
Identity = 367/383 (95.82%), Postives = 371/383 (96.87%), Query Frame = 0

Query: 74  MSPLKIFQASCDDKKQGIVAVLSSDPDRSKSQPSSLRRTLSADMSSHKWISQMKNVSSCQ 133
           MSPLKIFQASCDDKKQGIVAVLSSDPDRSKSQPSSLRRTLSADMSSHKWISQMKNV+SCQ
Sbjct: 1   MSPLKIFQASCDDKKQGIVAVLSSDPDRSKSQPSSLRRTLSADMSSHKWISQMKNVASCQ 60

Query: 134 EFCLWDSIQEREKKKKKTEEDKTE----SPDIWGSVIKSRKDFNANPYVPPRKMGKCLSE 193
           EF LWDSIQEREKKKKKTEEDKTE    S DIWGSVIKSRKDFNANPYVPPR MGKCLSE
Sbjct: 61  EFGLWDSIQEREKKKKKTEEDKTEDKTDSADIWGSVIKSRKDFNANPYVPPRPMGKCLSE 120

Query: 194 KSLEICTESLGSETGSDGFSSYPSSENGDSDEEEFEVEDQGKPVQYSYKKSPPRRSFPPP 253
           KSLEICTESLGSETGSDGFSSYPSSENGDSDEEEFEVEDQGKPV+YSYKKSPPRRSFPPP
Sbjct: 121 KSLEICTESLGSETGSDGFSSYPSSENGDSDEEEFEVEDQGKPVKYSYKKSPPRRSFPPP 180

Query: 254 LPTLSGPDGELVRMQPRRDNGRLVLEAMSVPSQSTFSAQRQDGRLVLTLVNRKPINEIKE 313
           LPTLSGPDGELVRMQPRRDNGRLVLEAMSVPSQSTFSAQRQDGRLVLTLVNRKPINEIKE
Sbjct: 181 LPTLSGPDGELVRMQPRRDNGRLVLEAMSVPSQSTFSAQRQDGRLVLTLVNRKPINEIKE 240

Query: 314 EEKKIENSSQLPSGLLKSHRLTSMMNSKSRVLANRNPSLPPRPKTAAAAAQPITPCAAPP 373
           E+KKIENSSQLPSGLLKSHRL SMMNSKSRVLANRNPSLPPRPK AAAAAQPITPCAAPP
Sbjct: 241 EQKKIENSSQLPSGLLKSHRLASMMNSKSRVLANRNPSLPPRPKAAAAAAQPITPCAAPP 300

Query: 374 TAASLNAYEYYWRSKPSSKAGAAAVNPLGQQAAAFKNSNRKIMICKNRAVYEEEQMLVMS 433
           TAASLNAYEYYWRSKPSSKAGAAAVNPLGQQAAAFKNSNRK+MICKNR V EEEQM VMS
Sbjct: 301 TAASLNAYEYYWRSKPSSKAGAAAVNPLGQQAAAFKNSNRKVMICKNRVVNEEEQMFVMS 360

Query: 434 PKGSCKEVRRTAVVWEPRCIATS 452
           PKGSCKEVRRTAVVWEPRCIATS
Sbjct: 361 PKGSCKEVRRTAVVWEPRCIATS 383

BLAST of Cp4.1LG04g12540.1 vs. ExPASy TrEMBL
Match: A0A0A0M0D7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G635920 PE=3 SV=1)

HSP 1 Score: 466 bits (1198), Expect = 1.11e-159
Identity = 267/407 (65.60%), Postives = 300/407 (73.71%), Query Frame = 0

Query: 74  MSPLKIFQAS-CDDKKQGIVAVLSSDPDRSKSQPSSLRRTLSADMSSHKWISQMKNVSSC 133
           MSPLKIFQAS  +DKKQGIVA LSSD D S+ QPSSLRRTLSADMSSHKWIS MK VSS 
Sbjct: 1   MSPLKIFQASYSNDKKQGIVAALSSDSDHSQIQPSSLRRTLSADMSSHKWISHMKKVSSF 60

Query: 134 QEFCLWDSIQEREKKKKKTEEDKTESPDIWGSVIKSRKDFNAN---PYVPP--RKMGKCL 193
           Q+F              KTE D+TE   +W S++K  +  + N   PYV P  ++ GKCL
Sbjct: 61  QDF-----------HNPKTE-DETEGVGVWSSIVKESRQESKNISDPYVHPLLKRSGKCL 120

Query: 194 SEKSLEICTESLGSETGSDGFSSYPSSENGDSDEEE----------FEVEDQGKPVQYSY 253
           SEKSLEICTESLGSETGSDGFSSYPSSENGD+D+E           FEVE+Q KPV+ SY
Sbjct: 121 SEKSLEICTESLGSETGSDGFSSYPSSENGDTDDENEENMIEGTQTFEVENQWKPVKISY 180

Query: 254 KKSPPRRSFPPPLPTLSGPDGELVRMQPRRDNGRLVLEAMSVPSQSTFSAQRQDGRLVLT 313
           KKSPPRRS PPPLPTLSGPDGE +RMQPRRDNGRLVLEAMSVPSQ+ F AQRQDGRLVLT
Sbjct: 181 KKSPPRRSLPPPLPTLSGPDGETLRMQPRRDNGRLVLEAMSVPSQNNFHAQRQDGRLVLT 240

Query: 314 LVNRKPINEI------------KEEEKKIENSSQLPSGLLKSHRLTSMMNSKSRVLANRN 373
           LV+R  IN++            KE E+K    S+ P GLL +HRL SMM+SK+  LANRN
Sbjct: 241 LVSRHLINQVEKIDDWKMEEEGKENEQKTTKLSEFPRGLLTTHRLASMMSSKTIALANRN 300

Query: 374 PSLPPRPKTAAAAAQPITPCAAPPTAASLNAYEYYWRSKPSSKAGAAAVNPLGQQAAAFK 433
           PSLPPRPK    AAQPI  C APP AASLNAYEYYWRSKP+ KAG     P+GQQ A FK
Sbjct: 301 PSLPPRPK----AAQPIISCVAPPVAASLNAYEYYWRSKPTGKAG----TPVGQQQATFK 360

Query: 434 NSNRKIMICKNRAVYEEEQMLVMSPKGSCKEVRRTAVVWEPRCIATS 452
           +S+RK+MI KNR   EEE+++VM   GSCKEVRRT V WEPRCIATS
Sbjct: 361 SSSRKVMISKNRTANEEEKLVVMCSNGSCKEVRRTVVFWEPRCIATS 387

BLAST of Cp4.1LG04g12540.1 vs. ExPASy TrEMBL
Match: A0A5A7V311 (Protein FAF-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1836G00110 PE=3 SV=1)

HSP 1 Score: 451 bits (1161), Expect = 4.53e-154
Identity = 264/407 (64.86%), Postives = 299/407 (73.46%), Query Frame = 0

Query: 74  MSPLKIFQAS-CDDKKQGIVAVLSSDPDRSKSQPSSLRRTLSADMSSHKWISQMKNVSSC 133
           MSPL IFQAS  +DKKQGIVAVLSSD D S+ Q SSLRRTLSADMSSHKWIS MK  SS 
Sbjct: 1   MSPLMIFQASYSNDKKQGIVAVLSSDSDHSQIQTSSLRRTLSADMSSHKWISLMKKTSSF 60

Query: 134 QEFCLWDSIQEREKKKKKTEEDKTESPDIWGSVIK-SRKDFNA--NPYVPP--RKMGKCL 193
           Q+F              KTE D+TE   +W S+IK SR+D     +PYV P  ++ GKCL
Sbjct: 61  QDF-----------HNPKTE-DETEGVGVWSSIIKESRQDSKPILDPYVHPLLKRSGKCL 120

Query: 194 SEKSLEICTESLGSETGSDGFSSYPSSENGDSDEEE----------FEVEDQGKPVQYSY 253
           SEKSLEICTESLGSETGSDGFSSYPSSENGD+D+E           FE+E+Q KPV+ S+
Sbjct: 121 SEKSLEICTESLGSETGSDGFSSYPSSENGDTDDENEENMIESTQTFEMENQWKPVKISH 180

Query: 254 KKSPPRRSFPPPLPTLSGPDGELVRMQPRRDNGRLVLEAMSVPSQSTFSAQRQDGRLVLT 313
           KKSPPRRS PPPLPTLSGPDGE +RMQPRRDNGRLVLEAMSVPSQ+ F AQRQDGRLVLT
Sbjct: 181 KKSPPRRSLPPPLPTLSGPDGETLRMQPRRDNGRLVLEAMSVPSQNNFHAQRQDGRLVLT 240

Query: 314 LVNRKPINEIK--------EEEK----KIENSSQLPSGLLKSHRLTSMMNSKSRVLANRN 373
           L+NR  IN+++        EEEK    K    S+ P GLL +HRL SMM+SK+  LANRN
Sbjct: 241 LINRHLINQVEKIDEWKMEEEEKGKEQKTTKLSEFPRGLLNTHRLASMMSSKTIALANRN 300

Query: 374 PSLPPRPKTAAAAAQPITPCAAPPTAASLNAYEYYWRSKPSSKAGAAAVNPLGQQAAAFK 433
           PSLPPRPK    A QPI  C APP  ASLNAYEYYWR+KP+ KAG     PLGQQ A FK
Sbjct: 301 PSLPPRPK----AGQPIIGCVAPPVVASLNAYEYYWRTKPTGKAG----TPLGQQPATFK 360

Query: 434 NSNRKIMICKNRAVYEEEQMLVMSPKGSCKEVRRTAVVWEPRCIATS 452
           +S+RK+MI KNR   EEE+++V+   GSCKEVRRT V WEPRCIATS
Sbjct: 361 SSSRKVMISKNRTANEEEKLVVLCSNGSCKEVRRTVVFWEPRCIATS 387

BLAST of Cp4.1LG04g12540.1 vs. ExPASy TrEMBL
Match: A0A1S3BPJ5 (protein FAF-like, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103491936 PE=3 SV=1)

HSP 1 Score: 451 bits (1161), Expect = 4.53e-154
Identity = 264/407 (64.86%), Postives = 299/407 (73.46%), Query Frame = 0

Query: 74  MSPLKIFQAS-CDDKKQGIVAVLSSDPDRSKSQPSSLRRTLSADMSSHKWISQMKNVSSC 133
           MSPL IFQAS  +DKKQGIVAVLSSD D S+ Q SSLRRTLSADMSSHKWIS MK  SS 
Sbjct: 1   MSPLMIFQASYSNDKKQGIVAVLSSDSDHSQIQTSSLRRTLSADMSSHKWISLMKKTSSF 60

Query: 134 QEFCLWDSIQEREKKKKKTEEDKTESPDIWGSVIK-SRKDFNA--NPYVPP--RKMGKCL 193
           Q+F              KTE D+TE   +W S+IK SR+D     +PYV P  ++ GKCL
Sbjct: 61  QDF-----------HNPKTE-DETEGVGVWSSIIKESRQDSKPILDPYVHPLLKRSGKCL 120

Query: 194 SEKSLEICTESLGSETGSDGFSSYPSSENGDSDEEE----------FEVEDQGKPVQYSY 253
           SEKSLEICTESLGSETGSDGFSSYPSSENGD+D+E           FE+E+Q KPV+ S+
Sbjct: 121 SEKSLEICTESLGSETGSDGFSSYPSSENGDTDDENEENMIESTQTFEMENQWKPVKISH 180

Query: 254 KKSPPRRSFPPPLPTLSGPDGELVRMQPRRDNGRLVLEAMSVPSQSTFSAQRQDGRLVLT 313
           KKSPPRRS PPPLPTLSGPDGE +RMQPRRDNGRLVLEAMSVPSQ+ F AQRQDGRLVLT
Sbjct: 181 KKSPPRRSLPPPLPTLSGPDGETLRMQPRRDNGRLVLEAMSVPSQNNFHAQRQDGRLVLT 240

Query: 314 LVNRKPINEIK--------EEEK----KIENSSQLPSGLLKSHRLTSMMNSKSRVLANRN 373
           L+NR  IN+++        EEEK    K    S+ P GLL +HRL SMM+SK+  LANRN
Sbjct: 241 LINRHLINQVEKIDEWKMEEEEKGKEQKTTKLSEFPRGLLNTHRLASMMSSKTIALANRN 300

Query: 374 PSLPPRPKTAAAAAQPITPCAAPPTAASLNAYEYYWRSKPSSKAGAAAVNPLGQQAAAFK 433
           PSLPPRPK    A QPI  C APP  ASLNAYEYYWR+KP+ KAG     PLGQQ A FK
Sbjct: 301 PSLPPRPK----AGQPIIGCVAPPVVASLNAYEYYWRTKPTGKAG----TPLGQQPATFK 360

Query: 434 NSNRKIMICKNRAVYEEEQMLVMSPKGSCKEVRRTAVVWEPRCIATS 452
           +S+RK+MI KNR   EEE+++V+   GSCKEVRRT V WEPRCIATS
Sbjct: 361 SSSRKVMISKNRTANEEEKLVVLCSNGSCKEVRRTVVFWEPRCIATS 387

BLAST of Cp4.1LG04g12540.1 vs. TAIR 10
Match: AT5G22090.1 (Protein of unknown function (DUF3049) )

HSP 1 Score: 164.1 bits (414), Expect = 2.6e-40
Identity = 157/466 (33.69%), Postives = 212/466 (45.49%), Query Frame = 0

Query: 87  KKQGIVAVLSSDPDRSKSQPSSLRRTLSADMSSHKWISQ-----MKNVSSCQEF------ 146
           K+QGIV++L      + S P SLRRT SAD+SS  W+SQ     MK +SS ++       
Sbjct: 18  KQQGIVSILGGISSNTSSAP-SLRRTFSADLSSKTWVSQNGFSPMKRISSSEKLRPDEEE 77

Query: 147 ---------CLWDSIQEREKKKKKTEEDKTESPDIWGSVI------KSRKDFNANPYVPP 206
                     +W  IQ+ +  KKK EE +    D+W S++      +S KD    PYV P
Sbjct: 78  AEEESRSGVDIWAQIQQDKNDKKKEEEIEPGQSDVWSSILSEKKKTESSKDTVPPPYVHP 137

Query: 207 -RKMGKCLSEKSLEICTESLGSETGSDGFSSYPSSENGDSD------------------- 266
             K    LSEKSLEICTESLGSETG DGFSS+ SSE GD++                   
Sbjct: 138 LMKRASSLSEKSLEICTESLGSETGCDGFSSHASSETGDAEIEIHDETNLVVNVTETKVE 197

Query: 267 ---EEEFEVEDQGKPVQYSYKKSPPRRSFPPPLPTLSGPDGELVRMQPRRDNGRLVLEAM 326
              E E  VE +   +  ++    P  SFPPP+ +LS   G  + M+ RRDNGRLVLEA+
Sbjct: 198 EITETEIVVEQESSIIVPNHIIELPPGSFPPPIRSLSSQSGSSLHMKTRRDNGRLVLEAV 257

Query: 327 SVPSQSTFSAQRQDGRLVLTL--VNRKP--------------INEIKEEEKKIENSSQ-- 386
           S+PS + FSA+RQDGRL+LT   ++ +P               +E +EEE++ E+  +  
Sbjct: 258 SMPSHNNFSAKRQDGRLLLTFAEISNEPNYDKEDEIDSEVQWFDEEEEEEEEEEDEEEEA 317

Query: 387 ------LPSGLLKS----------HRL----------------TSMMNSKSRVLANRNPS 446
                  P+GLL            HRL                    ++KS +      S
Sbjct: 318 PDEFAYKPNGLLYKMAQKPIPITVHRLAHKPIGVPKRNSRWPMADEFDTKSDLSTPVVHS 377

Query: 447 LPPRPKTAAAAAQPITPCAAPPT--AASLNAYEYYWRSKPSSKAGAAAVNPLGQQAAAFK 452
           LPPRP+ A  A     P     T  AA  N  +Y W+S  +   G         QA  F 
Sbjct: 378 LPPRPRVAQLARSTKPPSTVDDTVGAACFNTCDYSWKSTNTESFGPNTKTQF--QAQNFV 437

BLAST of Cp4.1LG04g12540.1 vs. TAIR 10
Match: AT5G22090.2 (Protein of unknown function (DUF3049) )

HSP 1 Score: 164.1 bits (414), Expect = 2.6e-40
Identity = 157/466 (33.69%), Postives = 212/466 (45.49%), Query Frame = 0

Query: 87  KKQGIVAVLSSDPDRSKSQPSSLRRTLSADMSSHKWISQ-----MKNVSSCQEF------ 146
           K+QGIV++L      + S P SLRRT SAD+SS  W+SQ     MK +SS ++       
Sbjct: 18  KQQGIVSILGGISSNTSSAP-SLRRTFSADLSSKTWVSQNGFSPMKRISSSEKLRPDEEE 77

Query: 147 ---------CLWDSIQEREKKKKKTEEDKTESPDIWGSVI------KSRKDFNANPYVPP 206
                     +W  IQ+ +  KKK EE +    D+W S++      +S KD    PYV P
Sbjct: 78  AEEESRSGVDIWAQIQQDKNDKKKEEEIEPGQSDVWSSILSEKKKTESSKDTVPPPYVHP 137

Query: 207 -RKMGKCLSEKSLEICTESLGSETGSDGFSSYPSSENGDSD------------------- 266
             K    LSEKSLEICTESLGSETG DGFSS+ SSE GD++                   
Sbjct: 138 LMKRASSLSEKSLEICTESLGSETGCDGFSSHASSETGDAEIEIHDETNLVVNVTETKVE 197

Query: 267 ---EEEFEVEDQGKPVQYSYKKSPPRRSFPPPLPTLSGPDGELVRMQPRRDNGRLVLEAM 326
              E E  VE +   +  ++    P  SFPPP+ +LS   G  + M+ RRDNGRLVLEA+
Sbjct: 198 EITETEIVVEQESSIIVPNHIIELPPGSFPPPIRSLSSQSGSSLHMKTRRDNGRLVLEAV 257

Query: 327 SVPSQSTFSAQRQDGRLVLTL--VNRKP--------------INEIKEEEKKIENSSQ-- 386
           S+PS + FSA+RQDGRL+LT   ++ +P               +E +EEE++ E+  +  
Sbjct: 258 SMPSHNNFSAKRQDGRLLLTFAEISNEPNYDKEDEIDSEVQWFDEEEEEEEEEEDEEEEA 317

Query: 387 ------LPSGLLKS----------HRL----------------TSMMNSKSRVLANRNPS 446
                  P+GLL            HRL                    ++KS +      S
Sbjct: 318 PDEFAYKPNGLLYKMAQKPIPITVHRLAHKPIGVPKRNSRWPMADEFDTKSDLSTPVVHS 377

Query: 447 LPPRPKTAAAAAQPITPCAAPPT--AASLNAYEYYWRSKPSSKAGAAAVNPLGQQAAAFK 452
           LPPRP+ A  A     P     T  AA  N  +Y W+S  +   G         QA  F 
Sbjct: 378 LPPRPRVAQLARSTKPPSTVDDTVGAACFNTCDYSWKSTNTESFGPNTKTQF--QAQNFV 437

BLAST of Cp4.1LG04g12540.1 vs. TAIR 10
Match: AT5G19260.1 (Protein of unknown function (DUF3049) )

HSP 1 Score: 59.7 bits (143), Expect = 7.0e-09
Identity = 70/233 (30.04%), Postives = 111/233 (47.64%), Query Frame = 0

Query: 97  SDPDRSKSQPSSLRRTLSADMSSHKWISQMKN-----VSSCQEFCLWDSIQEREKKKKKT 156
           S P+   SQP  L        SSH   ++ +N      S   +   W S+Q        +
Sbjct: 25  SSPNPHFSQPFGLALKSHLLDSSHAEDTRNRNDDKAAASPVSDSSGWSSLQSLSSGSSSS 84

Query: 157 EEDKTESPDIWGSVIKSRKDFNANPYVPPRKMGKCLSEKSLEICTESLGSETGSDG---- 216
            +  T S            +  ++ YV      + LS++SL +CTE+LGSE+GSD     
Sbjct: 85  TKTTTSS------------EKESSYYVQRPSSCRALSDQSLALCTENLGSESGSDVTDID 144

Query: 217 --FSSYPSSEN-GDSDEEEFEVEDQGKPVQYSYKKSPPRRSFPPPLPTLSGPDGELVRMQ 276
             FS    ++N G++  E   ++        S K+S      PPPL T+ G   + ++M+
Sbjct: 145 ELFSLDVQTKNLGETTTETRTLK--------SRKRSVSPSDLPPPLTTMRG--FQCIQMR 204

Query: 277 PRRDNGRLVLEAMSVPSQS-TFSAQRQDGRLVLTLVNRKPINE-IKEEEKKIE 316
           P R+NGRLV+ A + P ++  F A R +GRL L+++  K  NE ++ EE+ IE
Sbjct: 205 PHRENGRLVMTATNAPPRNGCFQADRSNGRLRLSIL--KDSNEFVENEEETIE 233

BLAST of Cp4.1LG04g12540.1 vs. TAIR 10
Match: AT5G22390.1 (Protein of unknown function (DUF3049) )

HSP 1 Score: 55.1 bits (131), Expect = 1.7e-07
Identity = 42/126 (33.33%), Postives = 68/126 (53.97%), Query Frame = 0

Query: 191 SLEICTESLGSETGSDGFSSYPSSENGDSDEEEFEV--EDQGKPVQYSYK-KSPPRRSFP 250
           SL++CTE LGSE+   G      + +GD D++E EV  +D G   +  ++ +   R+ +P
Sbjct: 78  SLQLCTEGLGSES-YYGLEDEKVNGDGDGDDDEIEVKGKDNGSNDEEEWEPRRKERKEYP 137

Query: 251 PPLPTLSGPDGELVRMQPRRDNGRLVLEAMSVPSQSTFSAQRQDGRLVLTLVNRKPINEI 310
           P +  +S         +  R  GRLVLE + +P +    A R+DGRL L LV  +  ++ 
Sbjct: 138 PAMTRMS--------FKTYRKEGRLVLEEVRIPRREFLRASREDGRLRLKLVQPEDDHDE 194

Query: 311 KEEEKK 314
           +EE +K
Sbjct: 198 EEENEK 194

BLAST of Cp4.1LG04g12540.1 vs. TAIR 10
Match: AT3G06020.1 (Protein of unknown function (DUF3049) )

HSP 1 Score: 50.8 bits (120), Expect = 3.3e-06
Identity = 43/138 (31.16%), Postives = 66/138 (47.83%), Query Frame = 0

Query: 185 KCLSEKSLEICTESLGSETGSDGFSSYPSSENGDSDEEEFEVEDQGKPVQYSYKKSPPRR 244
           + LS++SL +CTESLGSETGSD       S + +    E              +K     
Sbjct: 106 RTLSDESLALCTESLGSETGSDIIHEDMFSISSELQTMETRTTSTTSNPSRQDRKRNTMA 165

Query: 245 SFPPPLPTLSGPDGELVRMQPRRDNGRLVLEAMSVPSQSTFSAQRQDG--RLVLTLVNRK 304
           S PPPL ++ G D   + ++  R+NGRLV+ A   P ++     R +G  RL + + +  
Sbjct: 166 SLPPPLTSMIGFD--CIEVKSHRENGRLVMMATRPPPRNRCLQDRSNGCVRLAILIDSDD 225

Query: 305 PINEIKEEEKKIENSSQL 321
            I    +EEK+ E    +
Sbjct: 226 HIETETKEEKEEEEEETI 241

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q0V8653.7e-3933.69Protein FAF-like, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g22090 PE=... [more]
Q6NMR89.9e-0830.04Protein FANTASTIC FOUR 3 OS=Arabidopsis thaliana OX=3702 GN=FAF3 PE=2 SV=1[more]
Q9SFG64.6e-0531.16Protein FANTASTIC FOUR 4 OS=Arabidopsis thaliana OX=3702 GN=FAF4 PE=2 SV=1[more]
Q8GXU97.8e-0533.14Protein FANTASTIC FOUR 2 OS=Arabidopsis thaliana OX=3702 GN=FAF2 PE=2 SV=1[more]
Q9SY066.6e-0428.25Protein FANTASTIC FOUR 1 OS=Arabidopsis thaliana OX=3702 GN=FAF1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAG6588246.11.72e-31197.57Protein FAF-like, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7022163.11.80e-30897.13Protein FAF-like, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosp... [more]
XP_023529951.11.01e-266100.00protein FAF-like, chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_022924675.14.01e-25797.10protein FAF-like, chloroplastic [Cucurbita moschata][more]
XP_022972071.11.27e-25495.82protein FAF-like, chloroplastic [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1EFP61.94e-25797.10protein FAF-like, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111432104 PE... [more]
A0A6J1I4Y76.17e-25595.82protein FAF-like, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111470708 PE=3... [more]
A0A0A0M0D71.11e-15965.60Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G635920 PE=3 SV=1[more]
A0A5A7V3114.53e-15464.86Protein FAF-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1836G00... [more]
A0A1S3BPJ54.53e-15464.86protein FAF-like, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103491936 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT5G22090.12.6e-4033.69Protein of unknown function (DUF3049) [more]
AT5G22090.22.6e-4033.69Protein of unknown function (DUF3049) [more]
AT5G19260.17.0e-0930.04Protein of unknown function (DUF3049) [more]
AT5G22390.11.7e-0733.33Protein of unknown function (DUF3049) [more]
AT3G06020.13.3e-0631.16Protein of unknown function (DUF3049) [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021410The fantastic four familyPFAMPF11250FAFcoord: 245..298
e-value: 4.7E-18
score: 65.2
IPR021410The fantastic four familyPANTHERPTHR33155FANTASTIC FOUR-LIKE PROTEIN (DUF3049)coord: 82..451
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 199..261
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 199..215
NoneNo IPR availablePANTHERPTHR33155:SF3PROTEIN FAF-LIKE, CHLOROPLASTICcoord: 82..451

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cp4.1LG04g12540Cp4.1LG04g12540gene


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG04g12540.1:five_prime_utr:001Cp4.1LG04g12540.1:five_prime_utr:001five_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG04g12540.1:exon:001Cp4.1LG04g12540.1:exon:001exon
Cp4.1LG04g12540.1:exon:002Cp4.1LG04g12540.1:exon:002exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG04g12540.1:cds:002Cp4.1LG04g12540.1:cds:002CDS
Cp4.1LG04g12540.1:cds:001Cp4.1LG04g12540.1:cds:001CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG04g12540.1:three_prime_utr:001Cp4.1LG04g12540.1:three_prime_utr:001three_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cp4.1LG04g12540.1Cp4.1LG04g12540.1-proteinpolypeptide