Cp4.1LG04g10920.1 (mRNA) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG04g10920.1 vs. ExPASy Swiss-Prot
Match: Q9LYG9 (Mechanosensitive ion channel protein 10 OS=Arabidopsis thaliana OX=3702 GN=MSL10 PE=1 SV=1)

HSP 1 Score: 672.9 bits (1735), Expect = 4.4e-192
Identity = 372/718 (51.81%), Postives = 504/718 (70.19%), Query Frame = 0

Query: 90  RSFRCTIPKSVVG----SSSSHEIARM--NPYKPPKIPAESAV---RRPSFARSSFSKPK 149
           RS     P+S  G     S S EI+++  +P KPP+ P ++ V   +R SFARS +SKPK
Sbjct: 28  RSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPK 87

Query: 150 SRLVEPPCPHGGSLAEETFAEKSISGSPFCSSTKMDSPARITAVTSPREALKSVPTTPRT 209
           SR V+P CP   S+ EE   E+  +G  F  ++  +   R  +V SP       P TP  
Sbjct: 88  SRFVDPSCPVDTSILEEEVREQLGAGFSFSRASPNNKSNR--SVGSP------APVTPSK 147

Query: 210 PFTGNEEEDDEEVYRTAELKVKERSGKKLTKTILIEWFSFLCLTGCLISTLTVDKLATKE 269
                E+++DEE+Y+  +L  + RS  K++   LIE   F+ +   L+++LT++ L    
Sbjct: 148 VVV--EKDEDEEIYKKVKLNREMRS--KISTLALIESAFFVVILSALVASLTINVLKHHT 207

Query: 270 IWELRLWKWCVLVLVIFSGRLFSRWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIW 329
            W L +WKWCVLV+VIFSG L + WF+  +VFLIE NFLL+RKVLYFV+GL+KSV VFIW
Sbjct: 208 FWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVLYFVHGLKKSVQVFIW 267

Query: 330 LTLVLLAWGLLFDRTSKRSKKGHAILNYVTRALGGSLIGAAIWLVKTLLVKILAASFQCT 389
           L L+L+AW LLF+   KRS     +L  +TR L   L GA  WLVKTLL+KILAA+F   
Sbjct: 268 LCLILVAWILLFNHDVKRSPAATKVLKCITRTLISILTGAFFWLVKTLLLKILAANFNVN 327

Query: 390 RFFDRIQESIFHQYILRTLSGPPLMEMAKGIGRTAGTGQLSFRHLKKVRDGGKERKEEVI 449
            FFDRIQ+S+FHQY+L+TLSG PLME A+ +GR   TG LSF  + K    G  ++++VI
Sbjct: 328 NFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPSTGHLSFATVVK---KGTVKEKKVI 387

Query: 450 DVDKLKKMNQEKISAWTMRGLIHVIRSSGLSTISNTI-ENFRDEELEQKDKEIHSEWEAR 509
           D+ K+ KM +EK+SAWTMR L+  +R+SGLSTIS+T+ E    E  EQ D+EI SE EA 
Sbjct: 388 DMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEGKEQADREITSEMEAL 447

Query: 510 AAAYQIFRNVAKPGSKYIDEDDLFRFMSKEEVDNMLPLFEGAVETGKIKRKTLKNWLVNV 569
           AAAY +FRNVA+P   YI+E+DL RFM KEEVD + PLF+GA ETG+I RK    W+V V
Sbjct: 448 AAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAETGRITRKAFTEWVVKV 507

Query: 570 YVERKSLAHSLNDTKTAIEELNKLASATVAIVIIVEWLLMMGLLTTQVLVFFSSQLLLVV 629
           Y  R++LAHSLNDTKTA+++LNKL +A + +V +V WLL++ + TT+VL+FFS+QL+ + 
Sbjct: 508 YTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVATTKVLLFFSTQLVALA 567

Query: 630 FMFGNTARTVFEGIIFVFVMHPFDVGDRCVVDGVQLIVEEMNILTTIFLRCDNEKIFYPN 689
           F+ G+T + +FE I+FVFVMHP+DVGDRCVVDGV ++VEEMN+LTT+FL+ +NEK++YPN
Sbjct: 568 FIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPN 627

Query: 690 SVLATKPISNFYRSPEMGDSIEFSVDFSTSIESIGALKARIKSYIESKPQFWRPNHSILV 749
           +VLATKPISN++RSP MG+++EFS+ FST +  I  LK RI  Y+E  PQ W P HS++V
Sbjct: 628 AVLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSVVV 687

Query: 750 KEIENVNKMKMGLHVNHTINFQNYGDKSSRRSELVLELKKAFEDLGIKYHLLAQEVQL 798
           KEIEN+NK+KM L+ +HTI FQ   +++ RR+EL L +K+  EDL I Y LL Q++ L
Sbjct: 688 KEIENMNKLKMALYSDHTITFQENRERNLRRTELSLAIKRMLEDLHIDYTLLPQDINL 730

BLAST of Cp4.1LG04g10920.1 vs. ExPASy Swiss-Prot
Match: Q84M97 (Mechanosensitive ion channel protein 9 OS=Arabidopsis thaliana OX=3702 GN=MSL9 PE=1 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 1.3e-175
Identity = 334/688 (48.55%), Postives = 472/688 (68.60%), Query Frame = 0

Query: 115 YKPPKIPA-ESAVRRPSFARSSFSKPKSRLVEPPCPHGGSLAEE----TFAEKSISGSPF 174
           +KPPKIP+ E  VRR S +RS +SKPKSR  E       S  EE    +  E+  +GS  
Sbjct: 67  HKPPKIPSPEGLVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENGGRSLREQFGAGSFA 126

Query: 175 CSSTKMDSPARITAVTSPREALKSVPTTPRTPFTGNEEEDDEEVYRTAELKVKERSGKKL 234
             S    SP          ++ +SV +   +     E +++EE+Y+  +L   +RSG K 
Sbjct: 127 RGSFDRASP--------NNKSNRSVASAALSKVAEEEPDENEEIYKKVKLHRVKRSGMK- 186

Query: 235 TKTILIEWFSFLCLTGCLISTLTVDKLATKEIWELRLWKWCVLVLVIFSGRLFSRWFINC 294
                +E   F+ + G LI +LT+D +    IW L  WKWCVLV+V  SG L + WF++ 
Sbjct: 187 -PLAFLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLSGMLVTNWFMHF 246

Query: 295 LVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLTLVLLAWGLLFDRTSKRSKKGHAILNYV 354
           +VF+IE+N+LL++KVLYFV+GL+K+V VFIW +LVL+AW  LFD   KR++K    L+++
Sbjct: 247 VVFIIEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGDVKRTRKTKRFLDFI 306

Query: 355 TRALGGSLIGAAIWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLMEMAK 414
           T  +   L+G+ ++LVKT  +K+LA+ F    FF+RIQES+FHQY+L+TLSGPPL+E A+
Sbjct: 307 TWTIVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQTLSGPPLIEEAE 366

Query: 415 GIGRTAGTGQLSFRHLKKVRDGGKERKEEVIDVDKLKKMNQEKISAWTMRGLIHVIRSSG 474
            +GR   TG LSF   K     GK + ++VID+ K+ +M QEK+SAWTMR LI  + +SG
Sbjct: 367 NVGRVPSTGHLSFTRTK----DGKVKDKKVIDMGKVHRMKQEKVSAWTMRVLIEAVGTSG 426

Query: 475 LSTISNTIENFRDEELEQKDKEIHSEWEARAAAYQIFRNVAKPGSKYIDEDDLFRFMSKE 534
           +STIS+T++   +++ E+ DKEI +E EA AAAY +F NVAKP   YI+EDDL RFM KE
Sbjct: 427 ISTISSTLDEVNNKK-ERTDKEITNEMEAVAAAYDVFNNVAKPNHNYIEEDDLLRFMIKE 486

Query: 535 EVDNMLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASATVA 594
           EVD +LPL E A +TGKI RKT   W+VNVY  RK++ HSLNDTKTA+++L+KL +  + 
Sbjct: 487 EVDLVLPLIEDA-DTGKITRKTFTEWVVNVYTSRKTIGHSLNDTKTAVKQLDKLITGILT 546

Query: 595 IVIIVEWLLMMGLLTTQVLVFFSSQLLLVVFMFGNTARTVFEGIIFVFVMHPFDVGDRCV 654
           ++  + W++++ + +T++L+ FSSQ L + FM G+T + +FE  +FVFVMHP+DVGDRCV
Sbjct: 547 VITFIVWMVLLDIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFVMHPYDVGDRCV 606

Query: 655 VDGVQLIVEEMNILTTIFLRCDNEKIFYPNSVLATKPISNFYRSPEMGDSIEFSVDFSTS 714
           VDGV L+VEE+++LTT+FL+ DNEK+FYPNSVL +KPISNFYRSP+MGD ++F + FST 
Sbjct: 607 VDGVMLLVEEIDLLTTVFLKIDNEKVFYPNSVLISKPISNFYRSPDMGDYVDFGIAFSTP 666

Query: 715 IESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLHVNHTINFQNYGDKSSR 774
            E IG LK +I  Y+ +  Q W P   ++V+ IEN+NK+ + + V HTINFQ Y +KS R
Sbjct: 667 AEKIGCLKGKIGEYLVANSQHWYPEAQVMVRAIENMNKLVLNILVQHTINFQVYVEKSLR 726

Query: 775 RSELVLELKKAFEDLGIKYHLLAQEVQL 798
           R+ L++ +K+  EDL I Y LL Q+V L
Sbjct: 727 RTALIIAIKRILEDLEIDYTLLPQDVNL 738

BLAST of Cp4.1LG04g10920.1 vs. ExPASy Swiss-Prot
Match: Q9LH74 (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 PE=2 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 4.1e-145
Identity = 304/716 (42.46%), Postives = 444/716 (62.01%), Query Frame = 0

Query: 127 RRPSFARSSFSKPKSRLVEPPCPHGGSLAEETFAEKSISGSPFCSSTKMDSPARITAVTS 186
           ++P  +R   +K KSRL +PP P                  P    T+M S  R     S
Sbjct: 185 KKPMLSR---NKTKSRLQDPPTP----------------THPAIDKTEMKSGRRSGIFKS 244

Query: 187 PREALKSVPTTPRTPFTGNEEEDDEEVYRTAELKVKERSGKKLTKTILIEWFSFLCLTGC 246
               L   P        G EEE++E+ +   +L  +E    KL+  + +EW S + +   
Sbjct: 245 --GFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLP-EEFKRDKLSFWVFLEWISLVLIVTS 304

Query: 247 LISTLTVDKLATKEIWELRLWKWCVLVLVIFSGRLFSRWFINCLVFLIERNFLLKRKVLY 306
           L+ +LT+  L  K  W+L LWKW V VLV+  GRL S W +  +VFL+E+NF  +++VLY
Sbjct: 305 LVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRKRVLY 364

Query: 307 FVYGLRKSVIVFIWLTLVLLAWGLLFDRTSKRSKKGHAILNYVTRALGGSLIGAAIWLVK 366
           FVYG+RKSV   +WL LVLLAW  LFD+  +R  +  A L YVTR L   L+   IWLVK
Sbjct: 365 FVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTA-LRYVTRVLVCLLVALIIWLVK 424

Query: 367 TLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLMEM-------------AKGIGR 426
           T+LVK+LA+SF  + +FDRIQES+F QY++ TLSGPPLME+              K + +
Sbjct: 425 TILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQRMEEEEQQVAEDVKSLEK 484

Query: 427 TAGTG-----QLSFRHLKKV-------RDGGKERKE-EVIDVDKLKKMNQEKISAWTMRG 486
            AG       + + +   KV       R G K  ++ E I +D+LK+MN + +SAW M+ 
Sbjct: 485 LAGAKLPPALKATVKSFMKVGKSPGLNRIGSKRGEDGEGIRIDQLKRMNTKNVSAWNMKR 544

Query: 487 LIHVIRSSGLSTISNTIENFRDEELEQKD-KEIHSEWEARAAAYQIFRNVAKPGSKYIDE 546
           L+++I    +ST+    +N +D   E +D   I SE+EA+ AA +IF NV +PGS+YI  
Sbjct: 545 LMNIILKGAISTLD---QNMQDTTQEDEDATHIRSEYEAKCAARKIFHNVTEPGSRYIYL 604

Query: 547 DDLFRFMSKEEVDNMLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEE 606
           +D  RF+ +EE +  + LFEGA E+ KI +  LKNW+V  + ER++LA +LNDTKTA++ 
Sbjct: 605 EDFLRFLCEEEAERAMALFEGASESDKISKSCLKNWVVKAFRERRALALTLNDTKTAVDR 664

Query: 607 LNKLASATVAIVIIVEWLLMMGLLTTQVLVFFSSQLLLVVFMFGNTARTVFEGIIFVFVM 666
           L+++ +  + I+II+ WLL++G+ TT+ L+  SSQLLLV F+FGN+ +T+FE IIF+FVM
Sbjct: 665 LHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVM 724

Query: 667 HPFDVGDRCVVDGVQLIVEEMNILTTIFLRCDNEKIFYPNSVLATKPISNFYRSPEMGDS 726
           HPFDVGDRC +DGVQL+VEEMNILTT+FLR DN+KI YPNSVL TKPI+N+YRSP+MGD+
Sbjct: 725 HPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDA 784

Query: 727 IEFSVDFSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLHVNHTIN 786
           +EF V  +T  E I A+K RI SY+++K  +W P   I+   ++++N +K+ + + H +N
Sbjct: 785 VEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMN 844

Query: 787 FQNYGDKSSRRSELVLELKKAFEDLGIKYHLLAQEVQLNYV-----SSAAPMVPPS 811
            Q+ G++  RR  L+ E+ K   +L I+Y L    + +  +      +++  +PPS
Sbjct: 845 HQDMGERYIRRGLLLEEVGKTCRELDIEYRLYPLNINVRSLPPTANPTSSDRIPPS 874

BLAST of Cp4.1LG04g10920.1 vs. ExPASy Swiss-Prot
Match: Q9SYM1 (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 PE=1 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 5.9e-144
Identity = 288/644 (44.72%), Postives = 419/644 (65.06%), Query Frame = 0

Query: 199 RTPFTGNEEEDDEEVYRTAELKVKERSGKKLTKTILIEWFSFLCLTGCLISTLTVDKLAT 258
           ++P T  EEE+D+     AE   +E    KL+  I++EW S + +    + TL +  L  
Sbjct: 210 KSPKTQGEEEEDDPF--AAEDLPEEYRKDKLSLWIVLEWLSLILIIAGFVCTLAIPSLRK 269

Query: 259 KEIWELRLWKWCVLVLVIFSGRLFSRWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVF 318
           K++WEL+LWKW  +VLV+  GRL S W +  +VF IERNFLL+++VLYFVYG+RK+V   
Sbjct: 270 KKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFLLRKRVLYFVYGVRKAVQNC 329

Query: 319 IWLTLVLLAWGLLFDRTSKRSKKGHAILNYVTRALGGSLIGAAIWLVKTLLVKILAASFQ 378
           +WL LVLLAW  LFD    ++    A L  VT+     L+G  +WLVKTLLVK+LA+SF 
Sbjct: 330 LWLGLVLLAWHFLFDEKVAKAANTKA-LRVVTKIFVCLLVGFLLWLVKTLLVKVLASSFH 389

Query: 379 CTRFFDRIQESIFHQYILRTLSGPPLMEMAK-------------------GIGRTAG--- 438
            + +FDRIQES+F QY++ TLSGPPL+E+ K                   G+   +G   
Sbjct: 390 MSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEERISVEVKKFQNPGGVEIQSGAQK 449

Query: 439 ----TGQLSFRHLKKVRDGGKERKEEVIDVDKLKKMNQEKISAWTMRGLIHVIRSSGLST 498
               TG+  F        GG   + + I +D L K+N + +SAW M+ L+++IR+  L+T
Sbjct: 450 SPMKTGKSPFLSHVLSNGGGGGGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTT 509

Query: 499 ISNTIENFRDEEL-EQKDKEIHSEWEARAAAYQIFRNVAKPGSKYIDEDDLFRFMSKEEV 558
           +    E  +D  L + K  +I SE+EA+ AA +IF NVAKPGSK+I  +D+ RF+  +E 
Sbjct: 510 LD---EQLQDPSLDDDKGNQIRSEFEAKLAARKIFHNVAKPGSKFIYANDIMRFLPDDEA 569

Query: 559 DNMLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASATVAIV 618
              L LFEGA ET +I + +LKNW+VN + ER++LA +LNDTKTA+  L+K+ +  V I+
Sbjct: 570 LKTLSLFEGASETNRISKSSLKNWVVNAFRERRALALTLNDTKTAVNRLHKMVNIVVGII 629

Query: 619 IIVEWLLMMGLLTTQVLVFFSSQLLLVVFMFGNTARTVFEGIIFVFVMHPFDVGDRCVVD 678
           I+V WL+++G+ +T+ LV  SSQ+++V F+FGN  + VFE II++FV+HPFDVGDRC +D
Sbjct: 630 ILVIWLIILGITSTKFLVVMSSQVVVVAFIFGNMCKIVFESIIYLFVIHPFDVGDRCEID 689

Query: 679 GVQLIVEEMNILTTIFLRCDNEKIFYPNSVLATKPISNFYRSPEMGDSIEFSVDFSTSIE 738
           GVQ++VEEMNILTT+FLR DN+K+ YPNS+L TK I N+YRSP+MGD IEFS+  +T  E
Sbjct: 690 GVQMVVEEMNILTTVFLRFDNQKVVYPNSLLWTKSIGNYYRSPDMGDGIEFSIHITTPAE 749

Query: 739 SIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLHVNHTINFQNYGDKSSRRS 798
            I  +K RI SYIE K   W P   I+ K++E++N +++ +   H +N Q+ G+K +RRS
Sbjct: 750 KIILIKQRITSYIEGKKDHWYPAPMIVFKDMESLNSVRIAVWPTHRMNHQDMGEKWARRS 809

Query: 799 ELVLELKKAFEDLGIKYHLLAQEVQLNYV--SSAAPM---VPPS 811
           +LV E+ K   +L I+Y L   ++ +  +  S+A P+   +PP+
Sbjct: 810 QLVEEIAKICRELDIEYRLYPLDINVRNLPTSTALPVSDRLPPN 847

BLAST of Cp4.1LG04g10920.1 vs. ExPASy Swiss-Prot
Match: Q9LPG3 (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 PE=3 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 2.2e-143
Identity = 312/764 (40.84%), Postives = 459/764 (60.08%), Query Frame = 0

Query: 90  RSFRCTIPKSVV-GSSSSHEIARMNPYKPPKIPAESAVRRPSFARSSFSKPKSRLVEPPC 149
           R  R T+  SV  G SS  E  R++  +  K  +  +  R         K +SRL++PP 
Sbjct: 138 RQNRVTLSPSVKDGDSSEDEENRVDGSEVVKCTSNRSTMRTKTLMK--MKTRSRLMDPPT 197

Query: 150 PHGGSLAEETFAEKSISGSPFCSSTKMDSPARITAVTSPREALKSVPTTPRTPFTGNEEE 209
           P        T+ +     +P   +       R               T P TP  G  ++
Sbjct: 198 P--------TYPDMVSGRTPRSGNLNPGFSGR--------------NTKPGTPNQGGSKD 257

Query: 210 DDEEVYRTAELKVKERSGK-KLTKTILIEWFSFLCLTGCLISTLTVDKLATKEIWELRLW 269
            +EE    +E  + E   K K+   ++IEW   + +   LI +L +  L  K +W+L LW
Sbjct: 258 LEEEEDPFSEEDLPEGLRKEKICVWVIIEWIFLILIIASLICSLVIPYLRGKTLWDLALW 317

Query: 270 KWCVLVLVIFSGRLFSRWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLTLVLLA 329
           KW V+VLV+  GRL S W +   V+ +E NFL ++KVLYFVYG+RK V   +WL LVL+A
Sbjct: 318 KWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYFVYGIRKPVQNCLWLGLVLIA 377

Query: 330 WGLLFDRTSKRSKKGHAILNYVTRALGGSLIGAAIWLVKTLLVKILAASFQCTRFFDRIQ 389
           W  LFD+  +R  +   +L YVT+ L   L+   IWL+KTLLVK+LA+SF  + +FDRIQ
Sbjct: 378 WHFLFDKKVEREMRS-TVLKYVTKVLICLLVAVIIWLIKTLLVKVLASSFHMSTYFDRIQ 437

Query: 390 ESIFHQYILRTLSGPPLMEM-------------AKGIGR----------------TAGTG 449
           ES+F QY++ TLSGPP +E+              + +GR                T G+G
Sbjct: 438 ESLFTQYVIETLSGPPRIEIHIEEEKVANDVKTFEIVGRKLSPLGPKAVSSPPQVTVGSG 497

Query: 450 QLSFRHLKKV-------RDGG-KERKEEVIDVDKLKKMNQEKISAWTMRGLIHVIRSSGL 509
           +L  +   +V       R G  KE  EE I +D L++MN + +SAW M+ L++VI+   L
Sbjct: 498 RLQ-KSPSRVGKSPVLSRSGSKKEGGEEGIRIDHLQRMNTKNVSAWKMKKLMNVIKKGTL 557

Query: 510 STISNTIENFRDEELEQKDKEIHSEWEARAAAYQIFRNVAKPGSKYIDEDDLFRFMSKEE 569
           ST+   I++   +E + K  +I SE+EA+ AA +IF+NVA+PGS+YI  +D  RF+S++E
Sbjct: 558 STLDEQIQDTTTQE-DDKATQIRSEFEAKLAARKIFQNVAEPGSRYIYMEDFMRFLSEDE 617

Query: 570 VDNMLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASATVAI 629
            +  + LFEGA E  KI +  LKNW+VN + ER++LA +LNDTKTA+  L+++    V+I
Sbjct: 618 SERAMDLFEGASECHKISKSCLKNWVVNAFRERRALALTLNDTKTAVNRLHRIVDVLVSI 677

Query: 630 VIIVEWLLMMGLLTTQVLVFFSSQLLLVVFMFGNTARTVFEGIIFVFVMHPFDVGDRCVV 689
           VI++ WLL++G+ TT+ L+  SSQLLLVVF+FGN+ +T+FE +IFVFVMHPFDVGDRC +
Sbjct: 678 VILIIWLLILGIATTKFLLVISSQLLLVVFVFGNSCKTIFEAVIFVFVMHPFDVGDRCEI 737

Query: 690 DGVQLIVEEMNILTTIFLRCDNEKIFYPNSVLATKPISNFYRSPEMGDSIEFSVDFSTSI 749
           DGVQ+IVEEMNILTT+FLR DN+KI YPNS+L TKPI+N+YRSP+M D+IEF V  +T  
Sbjct: 738 DGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPIANYYRSPDMQDAIEFFVHIATPP 797

Query: 750 ESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLHVNHTINFQNYGDKSSRR 809
           E   AL+ RI SY+++K   W P+  I+ +++  +N +K+ +   H +N QN G++  RR
Sbjct: 798 EKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGLNSVKIAMWPTHKMNHQNMGERYVRR 857

Query: 810 SELVLELKKAFEDLGIKYHLLAQEVQLNYVSSAAPM----VPPS 811
            +L+ E+ +   +L I+Y L    + +  + +A P+    +PPS
Sbjct: 858 GQLLEEIGRLCRELDIEYRLYPLNINVKSLPAATPITSDRIPPS 874

BLAST of Cp4.1LG04g10920.1 vs. NCBI nr
Match: XP_023531580.1 (mechanosensitive ion channel protein 10-like [Cucurbita pepo subsp. pepo] >XP_023531581.1 mechanosensitive ion channel protein 10-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1473 bits (3813), Expect = 0.0
Identity = 767/767 (100.00%), Postives = 767/767 (100.00%), Query Frame = 0

Query: 46  MADKEEAKQVVLQILDCEDGGVGASKDLSKISVAKFPDFELKETRSFRCTIPKSVVGSSS 105
           MADKEEAKQVVLQILDCEDGGVGASKDLSKISVAKFPDFELKETRSFRCTIPKSVVGSSS
Sbjct: 1   MADKEEAKQVVLQILDCEDGGVGASKDLSKISVAKFPDFELKETRSFRCTIPKSVVGSSS 60

Query: 106 SHEIARMNPYKPPKIPAESAVRRPSFARSSFSKPKSRLVEPPCPHGGSLAEETFAEKSIS 165
           SHEIARMNPYKPPKIPAESAVRRPSFARSSFSKPKSRLVEPPCPHGGSLAEETFAEKSIS
Sbjct: 61  SHEIARMNPYKPPKIPAESAVRRPSFARSSFSKPKSRLVEPPCPHGGSLAEETFAEKSIS 120

Query: 166 GSPFCSSTKMDSPARITAVTSPREALKSVPTTPRTPFTGNEEEDDEEVYRTAELKVKERS 225
           GSPFCSSTKMDSPARITAVTSPREALKSVPTTPRTPFTGNEEEDDEEVYRTAELKVKERS
Sbjct: 121 GSPFCSSTKMDSPARITAVTSPREALKSVPTTPRTPFTGNEEEDDEEVYRTAELKVKERS 180

Query: 226 GKKLTKTILIEWFSFLCLTGCLISTLTVDKLATKEIWELRLWKWCVLVLVIFSGRLFSRW 285
           GKKLTKTILIEWFSFLCLTGCLISTLTVDKLATKEIWELRLWKWCVLVLVIFSGRLFSRW
Sbjct: 181 GKKLTKTILIEWFSFLCLTGCLISTLTVDKLATKEIWELRLWKWCVLVLVIFSGRLFSRW 240

Query: 286 FINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLTLVLLAWGLLFDRTSKRSKKGHAI 345
           FINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLTLVLLAWGLLFDRTSKRSKKGHAI
Sbjct: 241 FINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLTLVLLAWGLLFDRTSKRSKKGHAI 300

Query: 346 LNYVTRALGGSLIGAAIWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLM 405
           LNYVTRALGGSLIGAAIWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLM
Sbjct: 301 LNYVTRALGGSLIGAAIWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLM 360

Query: 406 EMAKGIGRTAGTGQLSFRHLKKVRDGGKERKEEVIDVDKLKKMNQEKISAWTMRGLIHVI 465
           EMAKGIGRTAGTGQLSFRHLKKVRDGGKERKEEVIDVDKLKKMNQEKISAWTMRGLIHVI
Sbjct: 361 EMAKGIGRTAGTGQLSFRHLKKVRDGGKERKEEVIDVDKLKKMNQEKISAWTMRGLIHVI 420

Query: 466 RSSGLSTISNTIENFRDEELEQKDKEIHSEWEARAAAYQIFRNVAKPGSKYIDEDDLFRF 525
           RSSGLSTISNTIENFRDEELEQKDKEIHSEWEARAAAYQIFRNVAKPGSKYIDEDDLFRF
Sbjct: 421 RSSGLSTISNTIENFRDEELEQKDKEIHSEWEARAAAYQIFRNVAKPGSKYIDEDDLFRF 480

Query: 526 MSKEEVDNMLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLAS 585
           MSKEEVDNMLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLAS
Sbjct: 481 MSKEEVDNMLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLAS 540

Query: 586 ATVAIVIIVEWLLMMGLLTTQVLVFFSSQLLLVVFMFGNTARTVFEGIIFVFVMHPFDVG 645
           ATVAIVIIVEWLLMMGLLTTQVLVFFSSQLLLVVFMFGNTARTVFEGIIFVFVMHPFDVG
Sbjct: 541 ATVAIVIIVEWLLMMGLLTTQVLVFFSSQLLLVVFMFGNTARTVFEGIIFVFVMHPFDVG 600

Query: 646 DRCVVDGVQLIVEEMNILTTIFLRCDNEKIFYPNSVLATKPISNFYRSPEMGDSIEFSVD 705
           DRCVVDGVQLIVEEMNILTTIFLRCDNEKIFYPNSVLATKPISNFYRSPEMGDSIEFSVD
Sbjct: 601 DRCVVDGVQLIVEEMNILTTIFLRCDNEKIFYPNSVLATKPISNFYRSPEMGDSIEFSVD 660

Query: 706 FSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLHVNHTINFQNYGD 765
           FSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLHVNHTINFQNYGD
Sbjct: 661 FSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLHVNHTINFQNYGD 720

Query: 766 KSSRRSELVLELKKAFEDLGIKYHLLAQEVQLNYVSSAAPMVPPSQR 812
           KSSRRSELVLELKKAFEDLGIKYHLLAQEVQLNYVSSAAPMVPPSQR
Sbjct: 721 KSSRRSELVLELKKAFEDLGIKYHLLAQEVQLNYVSSAAPMVPPSQR 767

BLAST of Cp4.1LG04g10920.1 vs. NCBI nr
Match: XP_022933478.1 (mechanosensitive ion channel protein 10-like [Cucurbita moschata] >XP_022933479.1 mechanosensitive ion channel protein 10-like [Cucurbita moschata])

HSP 1 Score: 1456 bits (3770), Expect = 0.0
Identity = 759/767 (98.96%), Postives = 763/767 (99.48%), Query Frame = 0

Query: 46  MADKEEAKQVVLQILDCEDGGVGASKDLSKISVAKFPDFELKETRSFRCTIPKSVVGSSS 105
           MADKEEAKQVVLQILD EDGGVGASKDLSKISVAKFPDFELKETRSFRCTIPKSVVGSSS
Sbjct: 1   MADKEEAKQVVLQILDYEDGGVGASKDLSKISVAKFPDFELKETRSFRCTIPKSVVGSSS 60

Query: 106 SHEIARMNPYKPPKIPAESAVRRPSFARSSFSKPKSRLVEPPCPHGGSLAEETFAEKSIS 165
           SHEIARMNPYKPPKIPAESAVRRPSFARSSFSKPKSRLVEPPCPHG +L EETFAEKSIS
Sbjct: 61  SHEIARMNPYKPPKIPAESAVRRPSFARSSFSKPKSRLVEPPCPHGVNLEEETFAEKSIS 120

Query: 166 GSPFCSSTKMDSPARITAVTSPREALKSVPTTPRTPFTGNEEEDDEEVYRTAELKVKERS 225
           GSPFCSSTKMDSPARITAVTSPREALKSVPTTPRTPFTGNEEEDDEEVYRTAELKVKERS
Sbjct: 121 GSPFCSSTKMDSPARITAVTSPREALKSVPTTPRTPFTGNEEEDDEEVYRTAELKVKERS 180

Query: 226 GKKLTKTILIEWFSFLCLTGCLISTLTVDKLATKEIWELRLWKWCVLVLVIFSGRLFSRW 285
           GKKLTKTILIEWFSFLCLTGCLI+TLTVDKLATKEIWELRLWKWCVLVLVIFSGRLFSRW
Sbjct: 181 GKKLTKTILIEWFSFLCLTGCLIATLTVDKLATKEIWELRLWKWCVLVLVIFSGRLFSRW 240

Query: 286 FINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLTLVLLAWGLLFDRTSKRSKKGHAI 345
           FINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLTLVLLAWGLLFDRTSKRSKKGHAI
Sbjct: 241 FINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLTLVLLAWGLLFDRTSKRSKKGHAI 300

Query: 346 LNYVTRALGGSLIGAAIWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLM 405
           L+YVTRALGGSLIGAAIWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLM
Sbjct: 301 LSYVTRALGGSLIGAAIWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLM 360

Query: 406 EMAKGIGRTAGTGQLSFRHLKKVRDGGKERKEEVIDVDKLKKMNQEKISAWTMRGLIHVI 465
           EMAKGIGRTAGTGQLSFRHLKKVRDGGKERKEEVIDVDKLKKM QEKISAWTMRGLIHVI
Sbjct: 361 EMAKGIGRTAGTGQLSFRHLKKVRDGGKERKEEVIDVDKLKKMKQEKISAWTMRGLIHVI 420

Query: 466 RSSGLSTISNTIENFRDEELEQKDKEIHSEWEARAAAYQIFRNVAKPGSKYIDEDDLFRF 525
           RSSGLSTISNTIENFRDEELEQKDKEIHSEWEARAAAYQIFRNVAKPGSKYIDEDDLFRF
Sbjct: 421 RSSGLSTISNTIENFRDEELEQKDKEIHSEWEARAAAYQIFRNVAKPGSKYIDEDDLFRF 480

Query: 526 MSKEEVDNMLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLAS 585
           MSKEEVDNMLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLAS
Sbjct: 481 MSKEEVDNMLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLAS 540

Query: 586 ATVAIVIIVEWLLMMGLLTTQVLVFFSSQLLLVVFMFGNTARTVFEGIIFVFVMHPFDVG 645
           ATVAIVII+EWLLMMGLLTTQVLVFFSSQLLLVVFMFGNTARTVFEGIIFVFVMHPFDVG
Sbjct: 541 ATVAIVIIIEWLLMMGLLTTQVLVFFSSQLLLVVFMFGNTARTVFEGIIFVFVMHPFDVG 600

Query: 646 DRCVVDGVQLIVEEMNILTTIFLRCDNEKIFYPNSVLATKPISNFYRSPEMGDSIEFSVD 705
           DRCVVDGVQLIVEEMNILTTIFLRCDNEKIFYPNSVLATKPISNFYRSPEMGDSIEFSVD
Sbjct: 601 DRCVVDGVQLIVEEMNILTTIFLRCDNEKIFYPNSVLATKPISNFYRSPEMGDSIEFSVD 660

Query: 706 FSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLHVNHTINFQNYGD 765
           FSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLHVNHTINFQNYGD
Sbjct: 661 FSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLHVNHTINFQNYGD 720

Query: 766 KSSRRSELVLELKKAFEDLGIKYHLLAQEVQLNYVSSAAPMVPPSQR 812
           KSSRRSELVLELKKAFEDLGIKYHLLAQEVQLNYVSSAAPMVPPSQR
Sbjct: 721 KSSRRSELVLELKKAFEDLGIKYHLLAQEVQLNYVSSAAPMVPPSQR 767

BLAST of Cp4.1LG04g10920.1 vs. NCBI nr
Match: XP_022966777.1 (mechanosensitive ion channel protein 10-like [Cucurbita maxima] >XP_022966786.1 mechanosensitive ion channel protein 10-like [Cucurbita maxima])

HSP 1 Score: 1454 bits (3764), Expect = 0.0
Identity = 756/767 (98.57%), Postives = 763/767 (99.48%), Query Frame = 0

Query: 46  MADKEEAKQVVLQILDCEDGGVGASKDLSKISVAKFPDFELKETRSFRCTIPKSVVGSSS 105
           MADKEEAKQVVLQILDCEDGGVGASKDLSKISVAKFPDFELKETRSFRCTIPKSVVGSSS
Sbjct: 1   MADKEEAKQVVLQILDCEDGGVGASKDLSKISVAKFPDFELKETRSFRCTIPKSVVGSSS 60

Query: 106 SHEIARMNPYKPPKIPAESAVRRPSFARSSFSKPKSRLVEPPCPHGGSLAEETFAEKSIS 165
           SHEI+RMNPYKPPKIPAESAVRRPSFARSSFSKPKSRLVEPPCPHG +LAEETFAEKSIS
Sbjct: 61  SHEISRMNPYKPPKIPAESAVRRPSFARSSFSKPKSRLVEPPCPHGVNLAEETFAEKSIS 120

Query: 166 GSPFCSSTKMDSPARITAVTSPREALKSVPTTPRTPFTGNEEEDDEEVYRTAELKVKERS 225
           GSPFCSSTKMDSPARITAVTSPREALKSVPTTPRTPFTGNEEEDDEEVYRTAELKVKERS
Sbjct: 121 GSPFCSSTKMDSPARITAVTSPREALKSVPTTPRTPFTGNEEEDDEEVYRTAELKVKERS 180

Query: 226 GKKLTKTILIEWFSFLCLTGCLISTLTVDKLATKEIWELRLWKWCVLVLVIFSGRLFSRW 285
           GKKLTKTILIEWFSFLCLTGCLI+TLTVDKLATKEIWELRLWKWCVLVLVIFSGRLFSRW
Sbjct: 181 GKKLTKTILIEWFSFLCLTGCLIATLTVDKLATKEIWELRLWKWCVLVLVIFSGRLFSRW 240

Query: 286 FINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLTLVLLAWGLLFDRTSKRSKKGHAI 345
           FINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLTLVLLAWGLLFDRTSKRSKKGHAI
Sbjct: 241 FINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLTLVLLAWGLLFDRTSKRSKKGHAI 300

Query: 346 LNYVTRALGGSLIGAAIWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLM 405
           LNYVTRALGGSLIGAAIWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLM
Sbjct: 301 LNYVTRALGGSLIGAAIWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLM 360

Query: 406 EMAKGIGRTAGTGQLSFRHLKKVRDGGKERKEEVIDVDKLKKMNQEKISAWTMRGLIHVI 465
           EMAKGIGRTAGTGQLSFRHLKKVRDGGKERKEEVIDVDKLKKM QEKISAWTMRGLIHVI
Sbjct: 361 EMAKGIGRTAGTGQLSFRHLKKVRDGGKERKEEVIDVDKLKKMKQEKISAWTMRGLIHVI 420

Query: 466 RSSGLSTISNTIENFRDEELEQKDKEIHSEWEARAAAYQIFRNVAKPGSKYIDEDDLFRF 525
           RSSGLSTISNTIENFR+EELEQKDKEIHSEWEARAAAYQIFRNVAKPGSKYIDEDDLFRF
Sbjct: 421 RSSGLSTISNTIENFREEELEQKDKEIHSEWEARAAAYQIFRNVAKPGSKYIDEDDLFRF 480

Query: 526 MSKEEVDNMLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLAS 585
           MSKEEVDNMLPLFEGAVETGKIKRKTLKNWLVNVY+ERKSLAHSLNDTKTAIEEL+KLAS
Sbjct: 481 MSKEEVDNMLPLFEGAVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELSKLAS 540

Query: 586 ATVAIVIIVEWLLMMGLLTTQVLVFFSSQLLLVVFMFGNTARTVFEGIIFVFVMHPFDVG 645
           ATV IVIIVEWLLMMGLLTTQ+LVFFSSQLLLVVFMFGNTARTVFEGIIFVFVMHPFDVG
Sbjct: 541 ATVVIVIIVEWLLMMGLLTTQILVFFSSQLLLVVFMFGNTARTVFEGIIFVFVMHPFDVG 600

Query: 646 DRCVVDGVQLIVEEMNILTTIFLRCDNEKIFYPNSVLATKPISNFYRSPEMGDSIEFSVD 705
           DRCVVDGVQLIVEEMNILTTIFLRCDNEKIFYPNSVLATKPISNFYRSPEMGDSIEFSVD
Sbjct: 601 DRCVVDGVQLIVEEMNILTTIFLRCDNEKIFYPNSVLATKPISNFYRSPEMGDSIEFSVD 660

Query: 706 FSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLHVNHTINFQNYGD 765
           FSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLHVNHTINFQNYGD
Sbjct: 661 FSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLHVNHTINFQNYGD 720

Query: 766 KSSRRSELVLELKKAFEDLGIKYHLLAQEVQLNYVSSAAPMVPPSQR 812
           KSSRRSELVLELKKAFEDLGIKYHLLAQEVQLNYVSSAA MVPPSQR
Sbjct: 721 KSSRRSELVLELKKAFEDLGIKYHLLAQEVQLNYVSSAALMVPPSQR 767

BLAST of Cp4.1LG04g10920.1 vs. NCBI nr
Match: KAG6588065.1 (Mechanosensitive ion channel protein 10, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1451 bits (3755), Expect = 0.0
Identity = 755/767 (98.44%), Postives = 761/767 (99.22%), Query Frame = 0

Query: 46  MADKEEAKQVVLQILDCEDGGVGASKDLSKISVAKFPDFELKETRSFRCTIPKSVVGSSS 105
           MADKEEAKQVVLQILD EDGGVG SKDLSKISVAKFPDFELKETRSFRCTIPKSVVGSSS
Sbjct: 1   MADKEEAKQVVLQILDYEDGGVGVSKDLSKISVAKFPDFELKETRSFRCTIPKSVVGSSS 60

Query: 106 SHEIARMNPYKPPKIPAESAVRRPSFARSSFSKPKSRLVEPPCPHGGSLAEETFAEKSIS 165
           SHEI+RMNP+KPPKIPAESAVRRPSFARSSFSKPKSRLVEPPCPHG +LAEETFAEKSIS
Sbjct: 61  SHEISRMNPFKPPKIPAESAVRRPSFARSSFSKPKSRLVEPPCPHGVNLAEETFAEKSIS 120

Query: 166 GSPFCSSTKMDSPARITAVTSPREALKSVPTTPRTPFTGNEEEDDEEVYRTAELKVKERS 225
           GSPFCSSTKMDSPARITA TSPREALKSVPTTPRTPFTGNEEEDDEEVYRTAELKVKERS
Sbjct: 121 GSPFCSSTKMDSPARITAATSPREALKSVPTTPRTPFTGNEEEDDEEVYRTAELKVKERS 180

Query: 226 GKKLTKTILIEWFSFLCLTGCLISTLTVDKLATKEIWELRLWKWCVLVLVIFSGRLFSRW 285
           GKKLTKTILIEWFSFLCLTGCLI+TLTVDKLATKEIWELRLWKWCVLVLVIFSGRLFSRW
Sbjct: 181 GKKLTKTILIEWFSFLCLTGCLIATLTVDKLATKEIWELRLWKWCVLVLVIFSGRLFSRW 240

Query: 286 FINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLTLVLLAWGLLFDRTSKRSKKGHAI 345
           FINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLTLVLLAWGLLFDRTSKRSKKGHAI
Sbjct: 241 FINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLTLVLLAWGLLFDRTSKRSKKGHAI 300

Query: 346 LNYVTRALGGSLIGAAIWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLM 405
           LNYVTRALGGSLIGAAIWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLM
Sbjct: 301 LNYVTRALGGSLIGAAIWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLM 360

Query: 406 EMAKGIGRTAGTGQLSFRHLKKVRDGGKERKEEVIDVDKLKKMNQEKISAWTMRGLIHVI 465
           EMAKGIGRTA TGQLSFRHLKKVRDGGKERKEEVIDVDKLKKMNQEKISAWTMRGLIHVI
Sbjct: 361 EMAKGIGRTASTGQLSFRHLKKVRDGGKERKEEVIDVDKLKKMNQEKISAWTMRGLIHVI 420

Query: 466 RSSGLSTISNTIENFRDEELEQKDKEIHSEWEARAAAYQIFRNVAKPGSKYIDEDDLFRF 525
           +SSGLSTISNTIENFRDEELEQKDKEIHSEWEARAAAYQIFRNVAKPGSKYI+EDDLFRF
Sbjct: 421 KSSGLSTISNTIENFRDEELEQKDKEIHSEWEARAAAYQIFRNVAKPGSKYIEEDDLFRF 480

Query: 526 MSKEEVDNMLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLAS 585
           MSKEEVDNMLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLAS
Sbjct: 481 MSKEEVDNMLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLAS 540

Query: 586 ATVAIVIIVEWLLMMGLLTTQVLVFFSSQLLLVVFMFGNTARTVFEGIIFVFVMHPFDVG 645
           ATV IVIIVEWLLMMGLLTTQVLVFFSSQLLLVVFMFGNTARTVFEGIIFVFVMHPFDVG
Sbjct: 541 ATVVIVIIVEWLLMMGLLTTQVLVFFSSQLLLVVFMFGNTARTVFEGIIFVFVMHPFDVG 600

Query: 646 DRCVVDGVQLIVEEMNILTTIFLRCDNEKIFYPNSVLATKPISNFYRSPEMGDSIEFSVD 705
           DRCVVDGVQLIVEEMNILTTIFLRCDNEKIFYPNSVLATKPISNFYRSPEMGDSIEFSVD
Sbjct: 601 DRCVVDGVQLIVEEMNILTTIFLRCDNEKIFYPNSVLATKPISNFYRSPEMGDSIEFSVD 660

Query: 706 FSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLHVNHTINFQNYGD 765
           FSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLHVNHTINFQNYGD
Sbjct: 661 FSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLHVNHTINFQNYGD 720

Query: 766 KSSRRSELVLELKKAFEDLGIKYHLLAQEVQLNYVSSAAPMVPPSQR 812
           KSSRRSELVLELKKAFEDLGIKYHLLAQEVQLNYVSSAAPMVPPSQR
Sbjct: 721 KSSRRSELVLELKKAFEDLGIKYHLLAQEVQLNYVSSAAPMVPPSQR 767

BLAST of Cp4.1LG04g10920.1 vs. NCBI nr
Match: XP_038878911.1 (mechanosensitive ion channel protein 10-like [Benincasa hispida])

HSP 1 Score: 1231 bits (3184), Expect = 0.0
Identity = 640/770 (83.12%), Postives = 699/770 (90.78%), Query Frame = 0

Query: 46  MADKEEAKQVVLQILDCEDGGVGASKDLSKISVAKFPDFELKETRSFRCTIPKSVVGSSS 105
           MADK+  +Q+VL+IL+ E+G V  SKDL+K SV+ FPDF+LKETRSFRCTIP+SVVGSS 
Sbjct: 1   MADKKGMEQLVLRILEGEEG-VDTSKDLTKPSVSSFPDFDLKETRSFRCTIPQSVVGSSP 60

Query: 106 SHEIARMNPYKPPKIPAESAVRRPSFARSSFSKPKSRLVEPPCPHGGSLAEETFAEKSIS 165
           SHEI+RM+P KPPKIP E+A+RRPSFARSSFSKPKSRL+E PCP   SLAEE    KS  
Sbjct: 61  SHEISRMSPLKPPKIPGETAIRRPSFARSSFSKPKSRLIESPCPDSASLAEEKAKAKST- 120

Query: 166 GSPFCSSTKMDSPARITAVTSPREALKSVPTTPRTPF---TGNEEEDDEEVYRTAELKVK 225
                SS KMDSPA+IT VTSP+EALKS P TP+TP    TG+EEEDDEEVY+TAELKVK
Sbjct: 121 ---LYSSPKMDSPAKITTVTSPKEALKSAPITPKTPLIGTTGSEEEDDEEVYKTAELKVK 180

Query: 226 ERSGKKLTKTILIEWFSFLCLTGCLISTLTVDKLATKEIWELRLWKWCVLVLVIFSGRLF 285
           ERSGKKL +T+LIEW +FLCLTGCLI++LT+DKL TKEIW L LWKWCVLVLV F GRLF
Sbjct: 181 ERSGKKLKRTVLIEWVAFLCLTGCLIASLTIDKLVTKEIWGLGLWKWCVLVLVSFCGRLF 240

Query: 286 SRWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLTLVLLAWGLLFDRTSKRSKKG 345
           S+WFINCLVFLIERNFLLKRKVLYFVYGLRKSVI+FIWL LVLLAWGLLFD++SKRSKKG
Sbjct: 241 SQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLGLVLLAWGLLFDQSSKRSKKG 300

Query: 346 HAILNYVTRALGGSLIGAAIWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGP 405
           + ILNY+TRALG SLIGA +WLVKTL+VKILAASFQC RFFDRIQESIFHQYILR LSGP
Sbjct: 301 NEILNYITRALGASLIGAGLWLVKTLMVKILAASFQCARFFDRIQESIFHQYILRILSGP 360

Query: 406 PLMEMAKGIGRTAGTGQLSFRHLKKVRDGGKERKEEVIDVDKLKKMNQEKISAWTMRGLI 465
           P+MEMA+ +GR A TGQLSFRHLKK  DGG E KEEVIDVDKLKKM QEKISAWTMRGLI
Sbjct: 361 PIMEMAESVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLI 420

Query: 466 HVIRSSGLSTISNTIENFRDEELEQKDKEIHSEWEARAAAYQIFRNVAKPGSKYIDEDDL 525
           +VIRSSGLSTISNTIENF++EE EQKDKEI+SEWEARAAAYQIFRNVAKPGSKYIDEDDL
Sbjct: 421 NVIRSSGLSTISNTIENFKEEENEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEDDL 480

Query: 526 FRFMSKEEVDNMLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNK 585
           FRFMSKEE+DN+LPLFEG VETGKIKRKTLKNWLVNVY+ERKSLAHSLNDTKTAIEELNK
Sbjct: 481 FRFMSKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNK 540

Query: 586 LASATVAIVIIVEWLLMMGLLTTQVLVFFSSQLLLVVFMFGNTARTVFEGIIFVFVMHPF 645
           LASA V IVII+EWLL+MG LTTQVLVF SSQ+LLVVFMFGNTARTVFE IIFVFVMHPF
Sbjct: 541 LASAIVLIVIIIEWLLLMGFLTTQVLVFVSSQILLVVFMFGNTARTVFEAIIFVFVMHPF 600

Query: 646 DVGDRCVVDGVQLIVEEMNILTTIFLRCDNEKIFYPNSVLATKPISNFYRSPEMGDSIEF 705
           DVGDRCVVDGVQ++VEEMNILTTIFLR DNEKIFYPNSVLATKPISN+YRSPEM DSIEF
Sbjct: 601 DVGDRCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIEF 660

Query: 706 SVDFSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLHVNHTINFQN 765
           SVDFSTSIESIGALKARIK+Y+ESKPQFWRPNHS++VKEIEN+NKMK+ L VNHTINFQN
Sbjct: 661 SVDFSTSIESIGALKARIKTYLESKPQFWRPNHSVIVKEIENINKMKLALCVNHTINFQN 720

Query: 766 YGDKSSRRSELVLELKKAFEDLGIKYHLLAQEVQLNYVSSAAPMVPPSQR 812
           YGDKSSRRS+LVLELKK FEDLGIKYHLL QEVQLNYV SAA ++P SQR
Sbjct: 721 YGDKSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVGSAASVIPSSQR 765

BLAST of Cp4.1LG04g10920.1 vs. ExPASy TrEMBL
Match: A0A6J1EZV8 (Mechanosensitive ion channel protein OS=Cucurbita moschata OX=3662 GN=LOC111440883 PE=3 SV=1)

HSP 1 Score: 1456 bits (3770), Expect = 0.0
Identity = 759/767 (98.96%), Postives = 763/767 (99.48%), Query Frame = 0

Query: 46  MADKEEAKQVVLQILDCEDGGVGASKDLSKISVAKFPDFELKETRSFRCTIPKSVVGSSS 105
           MADKEEAKQVVLQILD EDGGVGASKDLSKISVAKFPDFELKETRSFRCTIPKSVVGSSS
Sbjct: 1   MADKEEAKQVVLQILDYEDGGVGASKDLSKISVAKFPDFELKETRSFRCTIPKSVVGSSS 60

Query: 106 SHEIARMNPYKPPKIPAESAVRRPSFARSSFSKPKSRLVEPPCPHGGSLAEETFAEKSIS 165
           SHEIARMNPYKPPKIPAESAVRRPSFARSSFSKPKSRLVEPPCPHG +L EETFAEKSIS
Sbjct: 61  SHEIARMNPYKPPKIPAESAVRRPSFARSSFSKPKSRLVEPPCPHGVNLEEETFAEKSIS 120

Query: 166 GSPFCSSTKMDSPARITAVTSPREALKSVPTTPRTPFTGNEEEDDEEVYRTAELKVKERS 225
           GSPFCSSTKMDSPARITAVTSPREALKSVPTTPRTPFTGNEEEDDEEVYRTAELKVKERS
Sbjct: 121 GSPFCSSTKMDSPARITAVTSPREALKSVPTTPRTPFTGNEEEDDEEVYRTAELKVKERS 180

Query: 226 GKKLTKTILIEWFSFLCLTGCLISTLTVDKLATKEIWELRLWKWCVLVLVIFSGRLFSRW 285
           GKKLTKTILIEWFSFLCLTGCLI+TLTVDKLATKEIWELRLWKWCVLVLVIFSGRLFSRW
Sbjct: 181 GKKLTKTILIEWFSFLCLTGCLIATLTVDKLATKEIWELRLWKWCVLVLVIFSGRLFSRW 240

Query: 286 FINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLTLVLLAWGLLFDRTSKRSKKGHAI 345
           FINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLTLVLLAWGLLFDRTSKRSKKGHAI
Sbjct: 241 FINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLTLVLLAWGLLFDRTSKRSKKGHAI 300

Query: 346 LNYVTRALGGSLIGAAIWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLM 405
           L+YVTRALGGSLIGAAIWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLM
Sbjct: 301 LSYVTRALGGSLIGAAIWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLM 360

Query: 406 EMAKGIGRTAGTGQLSFRHLKKVRDGGKERKEEVIDVDKLKKMNQEKISAWTMRGLIHVI 465
           EMAKGIGRTAGTGQLSFRHLKKVRDGGKERKEEVIDVDKLKKM QEKISAWTMRGLIHVI
Sbjct: 361 EMAKGIGRTAGTGQLSFRHLKKVRDGGKERKEEVIDVDKLKKMKQEKISAWTMRGLIHVI 420

Query: 466 RSSGLSTISNTIENFRDEELEQKDKEIHSEWEARAAAYQIFRNVAKPGSKYIDEDDLFRF 525
           RSSGLSTISNTIENFRDEELEQKDKEIHSEWEARAAAYQIFRNVAKPGSKYIDEDDLFRF
Sbjct: 421 RSSGLSTISNTIENFRDEELEQKDKEIHSEWEARAAAYQIFRNVAKPGSKYIDEDDLFRF 480

Query: 526 MSKEEVDNMLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLAS 585
           MSKEEVDNMLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLAS
Sbjct: 481 MSKEEVDNMLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLAS 540

Query: 586 ATVAIVIIVEWLLMMGLLTTQVLVFFSSQLLLVVFMFGNTARTVFEGIIFVFVMHPFDVG 645
           ATVAIVII+EWLLMMGLLTTQVLVFFSSQLLLVVFMFGNTARTVFEGIIFVFVMHPFDVG
Sbjct: 541 ATVAIVIIIEWLLMMGLLTTQVLVFFSSQLLLVVFMFGNTARTVFEGIIFVFVMHPFDVG 600

Query: 646 DRCVVDGVQLIVEEMNILTTIFLRCDNEKIFYPNSVLATKPISNFYRSPEMGDSIEFSVD 705
           DRCVVDGVQLIVEEMNILTTIFLRCDNEKIFYPNSVLATKPISNFYRSPEMGDSIEFSVD
Sbjct: 601 DRCVVDGVQLIVEEMNILTTIFLRCDNEKIFYPNSVLATKPISNFYRSPEMGDSIEFSVD 660

Query: 706 FSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLHVNHTINFQNYGD 765
           FSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLHVNHTINFQNYGD
Sbjct: 661 FSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLHVNHTINFQNYGD 720

Query: 766 KSSRRSELVLELKKAFEDLGIKYHLLAQEVQLNYVSSAAPMVPPSQR 812
           KSSRRSELVLELKKAFEDLGIKYHLLAQEVQLNYVSSAAPMVPPSQR
Sbjct: 721 KSSRRSELVLELKKAFEDLGIKYHLLAQEVQLNYVSSAAPMVPPSQR 767

BLAST of Cp4.1LG04g10920.1 vs. ExPASy TrEMBL
Match: A0A6J1HNW1 (Mechanosensitive ion channel protein OS=Cucurbita maxima OX=3661 GN=LOC111466384 PE=3 SV=1)

HSP 1 Score: 1454 bits (3764), Expect = 0.0
Identity = 756/767 (98.57%), Postives = 763/767 (99.48%), Query Frame = 0

Query: 46  MADKEEAKQVVLQILDCEDGGVGASKDLSKISVAKFPDFELKETRSFRCTIPKSVVGSSS 105
           MADKEEAKQVVLQILDCEDGGVGASKDLSKISVAKFPDFELKETRSFRCTIPKSVVGSSS
Sbjct: 1   MADKEEAKQVVLQILDCEDGGVGASKDLSKISVAKFPDFELKETRSFRCTIPKSVVGSSS 60

Query: 106 SHEIARMNPYKPPKIPAESAVRRPSFARSSFSKPKSRLVEPPCPHGGSLAEETFAEKSIS 165
           SHEI+RMNPYKPPKIPAESAVRRPSFARSSFSKPKSRLVEPPCPHG +LAEETFAEKSIS
Sbjct: 61  SHEISRMNPYKPPKIPAESAVRRPSFARSSFSKPKSRLVEPPCPHGVNLAEETFAEKSIS 120

Query: 166 GSPFCSSTKMDSPARITAVTSPREALKSVPTTPRTPFTGNEEEDDEEVYRTAELKVKERS 225
           GSPFCSSTKMDSPARITAVTSPREALKSVPTTPRTPFTGNEEEDDEEVYRTAELKVKERS
Sbjct: 121 GSPFCSSTKMDSPARITAVTSPREALKSVPTTPRTPFTGNEEEDDEEVYRTAELKVKERS 180

Query: 226 GKKLTKTILIEWFSFLCLTGCLISTLTVDKLATKEIWELRLWKWCVLVLVIFSGRLFSRW 285
           GKKLTKTILIEWFSFLCLTGCLI+TLTVDKLATKEIWELRLWKWCVLVLVIFSGRLFSRW
Sbjct: 181 GKKLTKTILIEWFSFLCLTGCLIATLTVDKLATKEIWELRLWKWCVLVLVIFSGRLFSRW 240

Query: 286 FINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLTLVLLAWGLLFDRTSKRSKKGHAI 345
           FINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLTLVLLAWGLLFDRTSKRSKKGHAI
Sbjct: 241 FINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLTLVLLAWGLLFDRTSKRSKKGHAI 300

Query: 346 LNYVTRALGGSLIGAAIWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLM 405
           LNYVTRALGGSLIGAAIWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLM
Sbjct: 301 LNYVTRALGGSLIGAAIWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLM 360

Query: 406 EMAKGIGRTAGTGQLSFRHLKKVRDGGKERKEEVIDVDKLKKMNQEKISAWTMRGLIHVI 465
           EMAKGIGRTAGTGQLSFRHLKKVRDGGKERKEEVIDVDKLKKM QEKISAWTMRGLIHVI
Sbjct: 361 EMAKGIGRTAGTGQLSFRHLKKVRDGGKERKEEVIDVDKLKKMKQEKISAWTMRGLIHVI 420

Query: 466 RSSGLSTISNTIENFRDEELEQKDKEIHSEWEARAAAYQIFRNVAKPGSKYIDEDDLFRF 525
           RSSGLSTISNTIENFR+EELEQKDKEIHSEWEARAAAYQIFRNVAKPGSKYIDEDDLFRF
Sbjct: 421 RSSGLSTISNTIENFREEELEQKDKEIHSEWEARAAAYQIFRNVAKPGSKYIDEDDLFRF 480

Query: 526 MSKEEVDNMLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLAS 585
           MSKEEVDNMLPLFEGAVETGKIKRKTLKNWLVNVY+ERKSLAHSLNDTKTAIEEL+KLAS
Sbjct: 481 MSKEEVDNMLPLFEGAVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELSKLAS 540

Query: 586 ATVAIVIIVEWLLMMGLLTTQVLVFFSSQLLLVVFMFGNTARTVFEGIIFVFVMHPFDVG 645
           ATV IVIIVEWLLMMGLLTTQ+LVFFSSQLLLVVFMFGNTARTVFEGIIFVFVMHPFDVG
Sbjct: 541 ATVVIVIIVEWLLMMGLLTTQILVFFSSQLLLVVFMFGNTARTVFEGIIFVFVMHPFDVG 600

Query: 646 DRCVVDGVQLIVEEMNILTTIFLRCDNEKIFYPNSVLATKPISNFYRSPEMGDSIEFSVD 705
           DRCVVDGVQLIVEEMNILTTIFLRCDNEKIFYPNSVLATKPISNFYRSPEMGDSIEFSVD
Sbjct: 601 DRCVVDGVQLIVEEMNILTTIFLRCDNEKIFYPNSVLATKPISNFYRSPEMGDSIEFSVD 660

Query: 706 FSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLHVNHTINFQNYGD 765
           FSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLHVNHTINFQNYGD
Sbjct: 661 FSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLHVNHTINFQNYGD 720

Query: 766 KSSRRSELVLELKKAFEDLGIKYHLLAQEVQLNYVSSAAPMVPPSQR 812
           KSSRRSELVLELKKAFEDLGIKYHLLAQEVQLNYVSSAA MVPPSQR
Sbjct: 721 KSSRRSELVLELKKAFEDLGIKYHLLAQEVQLNYVSSAALMVPPSQR 767

BLAST of Cp4.1LG04g10920.1 vs. ExPASy TrEMBL
Match: A0A6J1JDR1 (Mechanosensitive ion channel protein OS=Cucurbita maxima OX=3661 GN=LOC111484882 PE=3 SV=1)

HSP 1 Score: 1212 bits (3135), Expect = 0.0
Identity = 634/770 (82.34%), Postives = 688/770 (89.35%), Query Frame = 0

Query: 46  MADKEEAKQVVLQILDCEDGGVGASKDLSKISVAKFPDFELKETRSFRCTIPKSVVGSSS 105
           MADK+  +QVVL+ILD E+G V A   L+  SVA FPDFE KETRS RC IP+SVV SS 
Sbjct: 1   MADKKGTEQVVLRILDGEEG-VDARNGLTNGSVASFPDFESKETRSVRCAIPQSVVESSP 60

Query: 106 SHEIARMNPYKPPKIPAESAVRRPSFARSSFSKPKSRLVEPPCPHGGSLAEETFAEKSIS 165
           SHEI+RM+  KPPKIP ESAVRRPSFARSSFSKPKSRL+EPPCP G S AEE    KS S
Sbjct: 61  SHEISRMSSLKPPKIPVESAVRRPSFARSSFSKPKSRLIEPPCPDGASSAEENAQAKSTS 120

Query: 166 GSPFCSSTKMDSPARITAVTSPREALKSVPTTPRTPF---TGNEEEDDEEVYRTAELKVK 225
           GSPF SS KMDSPA+I   TSP+E+LKS P TPRTP    TG+EEEDDEEVY+TAELKVK
Sbjct: 121 GSPFSSSPKMDSPAKIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVK 180

Query: 226 ERSGKKLTKTILIEWFSFLCLTGCLISTLTVDKLATKEIWELRLWKWCVLVLVIFSGRLF 285
           E+S KKL + +LIEW +FLC+T CLIS+LT+DKL TKEIW L LWKWCVLVLVIF GRLF
Sbjct: 181 EKSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLF 240

Query: 286 SRWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLTLVLLAWGLLFDRTSKRSKKG 345
           S+WFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWL LVLLAWGLLFD++ KRSKK 
Sbjct: 241 SQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLALVLLAWGLLFDQSIKRSKKV 300

Query: 346 HAILNYVTRALGGSLIGAAIWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGP 405
           + ILNYVTRAL  SLIGA +WLVKTLLVKILAASFQCTRFFDRIQESIFHQYILR LSGP
Sbjct: 301 NEILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGP 360

Query: 406 PLMEMAKGIGRTAGTGQLSFRHLKKVRDGGKERKEEVIDVDKLKKMNQEKISAWTMRGLI 465
           PLMEMA+ +GR A  GQLSFRHLKK RDGG E KEEVIDVDKLKKM Q K+SAWTMRGLI
Sbjct: 361 PLMEMAERVGRVASAGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQAKVSAWTMRGLI 420

Query: 466 HVIRSSGLSTISNTIENFRDEELEQKDKEIHSEWEARAAAYQIFRNVAKPGSKYIDEDDL 525
           +VIRSSGLSTISNTIENF++EE EQKDKEI+SEWEA AAAYQIFRNVAKPGSKYIDE+DL
Sbjct: 421 NVIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDL 480

Query: 526 FRFMSKEEVDNMLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNK 585
           FRFM+KEE+DN+LPLFEG VETGKIKRKTLKNWLVNVY+ERKSLAHSLNDTKTAIEELN+
Sbjct: 481 FRFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNR 540

Query: 586 LASATVAIVIIVEWLLMMGLLTTQVLVFFSSQLLLVVFMFGNTARTVFEGIIFVFVMHPF 645
           LASA V IVII+ WLL+MG LTTQVLVF SSQLLLVVFMFGNTA+T+FE IIFVFVMHPF
Sbjct: 541 LASAFVLIVIIIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPF 600

Query: 646 DVGDRCVVDGVQLIVEEMNILTTIFLRCDNEKIFYPNSVLATKPISNFYRSPEMGDSIEF 705
           DVGDRCVVDGVQ+IVEEMNILTTIFLR DNEKIFYPNSVLATKPISNFYRSPEM DSI+F
Sbjct: 601 DVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDF 660

Query: 706 SVDFSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLHVNHTINFQN 765
           SVDFSTSIESIGALKARIKSY+ESKPQFWRPNHS++VKEIENVNKMK+ L++NHTINFQN
Sbjct: 661 SVDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQN 720

Query: 766 YGDKSSRRSELVLELKKAFEDLGIKYHLLAQEVQLNYVSSAAPMVPPSQR 812
           YGD+SSRRS+LVLELKK FEDLGIKYHLL QEVQLNYVSS APMV  + R
Sbjct: 721 YGDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSSIAPMVSTTSR 769

BLAST of Cp4.1LG04g10920.1 vs. ExPASy TrEMBL
Match: A0A1S3BR08 (Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103492355 PE=3 SV=1)

HSP 1 Score: 1209 bits (3129), Expect = 0.0
Identity = 632/773 (81.76%), Postives = 696/773 (90.04%), Query Frame = 0

Query: 46  MADKEEAKQVVLQILDCEDGGVGASKDLSKISVAKFPDFELKETRSFRCTIPKSVVGSSS 105
           MADK+  +Q+VL+IL+ E+G  G+SKDL+K SV  FPDF+LKETRSFRCTIP+S+VGSS 
Sbjct: 1   MADKKGMEQLVLRILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPRSLVGSSP 60

Query: 106 SHEIARMNPYKPPKIPAESAVRRPSFARSSFSKPKSRLVEPPCPHGGSLAEE-TFAEKSI 165
           SHEI+RM P KPPKIP E+  RR SFA SSFSKPKSRL+EPPCP G SLAEE   A+ S+
Sbjct: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIEPPCPDGESLAEEKALAKSSL 120

Query: 166 SGSPFCSSTKMDSPARITAVTSPREALKSVPTTPRTPF---TGNEEEDDEEVYRTAELKV 225
            GSP     K+DSPA+ITAVTSP+EALK+ P TP+TP    TGNEEEDDEEVY+TAELKV
Sbjct: 121 YGSP-----KVDSPAKITAVTSPKEALKATPITPKTPLIGTTGNEEEDDEEVYKTAELKV 180

Query: 226 KERSGKKLTKTILIEWFSFLCLTGCLISTLTVDKLATKEIWELRLWKWCVLVLVIFSGRL 285
           KE+S K+L KT+++EW +FLCLTGCLI++LT+D L TKEIW L LWKWCVLVLVIF GRL
Sbjct: 181 KEKSEKRLKKTVIVEWVAFLCLTGCLIASLTIDTLVTKEIWGLGLWKWCVLVLVIFCGRL 240

Query: 286 FSRWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLTLVLLAWGLLFDRTSKRSKK 345
           FS+WFINCLVFLIERNFLLKRKVLYFVYGL+KSVI+FIWL LVLLAWGLLFD++SKRSKK
Sbjct: 241 FSQWFINCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKK 300

Query: 346 GHAILNYVTRALGGSLIGAAIWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSG 405
           G+ ILNYVTRALG SLIGA +WLVKTLLVKILAASFQCTRFFDRIQESIFHQYILR LSG
Sbjct: 301 GNEILNYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSG 360

Query: 406 PPLMEMAKGIGRTAGTGQLSFRHLKKVRDGGKERKEEVIDVDKLKKMNQEKISAWTMRGL 465
           PPLMEMA  +GR A TGQLSF+HLK+  D G E KEEVIDVDKLKKM QEKISAWTMRGL
Sbjct: 361 PPLMEMAGRVGRAASTGQLSFKHLKQESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGL 420

Query: 466 IHVIRSSGLSTISNTIENFRDEELEQKDKEIHSEWEARAAAYQIFRNVAKPGSKYIDEDD 525
           I+VIR SGLSTISNTIENF++EE+EQKDKEI+SEWEARAAAYQIFRNVAKPGSKYIDE+D
Sbjct: 421 INVIRGSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEED 480

Query: 526 LFRFMSKEEVDNMLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELN 585
           LFRFMSKEE+DN+LPLFEG  ETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELN
Sbjct: 481 LFRFMSKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELN 540

Query: 586 KLASATVAIVIIVEWLLMMGLLTTQVLVFFSSQLLLVVFMFGNTARTVFEGIIFVFVMHP 645
           KLASA V IVII+EWLL+MG LTTQVLVF SSQ+LLVVFMFGNTARTVFE IIFVFVMHP
Sbjct: 541 KLASAVVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHP 600

Query: 646 FDVGDRCVVDGVQLIVEEMNILTTIFLRCDNEKIFYPNSVLATKPISNFYRSPEMGDSIE 705
           FDVGDRCVVDGVQ++VEEMNILTTIFLR DNEKIFYPNSVLATKPISN+YRSPEM DSI+
Sbjct: 601 FDVGDRCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSID 660

Query: 706 FSVDFSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLHVNHTINFQ 765
           FSVDFSTSIESIGALKARIK+Y+ESKPQFWRPN+S++VKEIENVNKMK+ L VNHTINFQ
Sbjct: 661 FSVDFSTSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQ 720

Query: 766 NYGDKSSRRSELVLELKKAFEDLGIKYHLLAQEVQLNYVSSAAPMVP--PSQR 812
           NYGDKS+RRS+LVLELKK FEDLGIKYHLL Q VQLNY SSAA  VP  PSQR
Sbjct: 721 NYGDKSNRRSDLVLELKKIFEDLGIKYHLLPQAVQLNYASSAAGGVPLPPSQR 768

BLAST of Cp4.1LG04g10920.1 vs. ExPASy TrEMBL
Match: A0A5D3CFQ7 (Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001770 PE=3 SV=1)

HSP 1 Score: 1209 bits (3129), Expect = 0.0
Identity = 632/773 (81.76%), Postives = 696/773 (90.04%), Query Frame = 0

Query: 46  MADKEEAKQVVLQILDCEDGGVGASKDLSKISVAKFPDFELKETRSFRCTIPKSVVGSSS 105
           MADK+  +Q+VL+IL+ E+G  G+SKDL+K SV  FPDF+LKETRSFRCTIP+S+VGSS 
Sbjct: 1   MADKKGMEQLVLRILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPRSLVGSSP 60

Query: 106 SHEIARMNPYKPPKIPAESAVRRPSFARSSFSKPKSRLVEPPCPHGGSLAEE-TFAEKSI 165
           SHEI+RM P KPPKIP E+  RR SFA SSFSKPKSRL+EPPCP G SLAEE   A+ S+
Sbjct: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIEPPCPDGESLAEEKALAKSSL 120

Query: 166 SGSPFCSSTKMDSPARITAVTSPREALKSVPTTPRTPF---TGNEEEDDEEVYRTAELKV 225
            GSP     K+DSPA+ITAVTSP+EALK+ P TP+TP    TGNEEEDDEEVY+TAELKV
Sbjct: 121 YGSP-----KVDSPAKITAVTSPKEALKATPITPKTPLIGTTGNEEEDDEEVYKTAELKV 180

Query: 226 KERSGKKLTKTILIEWFSFLCLTGCLISTLTVDKLATKEIWELRLWKWCVLVLVIFSGRL 285
           KE+S K+L KT+++EW +FLCLTGCLI++LT+D L TKEIW L LWKWCVLVLVIF GRL
Sbjct: 181 KEKSEKRLKKTVIVEWVAFLCLTGCLIASLTIDTLVTKEIWGLGLWKWCVLVLVIFCGRL 240

Query: 286 FSRWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLTLVLLAWGLLFDRTSKRSKK 345
           FS+WFINCLVFLIERNFLLKRKVLYFVYGL+KSVI+FIWL LVLLAWGLLFD++SKRSKK
Sbjct: 241 FSQWFINCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKK 300

Query: 346 GHAILNYVTRALGGSLIGAAIWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSG 405
           G+ ILNYVTRALG SLIGA +WLVKTLLVKILAASFQCTRFFDRIQESIFHQYILR LSG
Sbjct: 301 GNEILNYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSG 360

Query: 406 PPLMEMAKGIGRTAGTGQLSFRHLKKVRDGGKERKEEVIDVDKLKKMNQEKISAWTMRGL 465
           PPLMEMA  +GR A TGQLSF+HLK+  D G E KEEVIDVDKLKKM QEKISAWTMRGL
Sbjct: 361 PPLMEMAGRVGRAASTGQLSFKHLKQESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGL 420

Query: 466 IHVIRSSGLSTISNTIENFRDEELEQKDKEIHSEWEARAAAYQIFRNVAKPGSKYIDEDD 525
           I+VIR SGLSTISNTIENF++EE+EQKDKEI+SEWEARAAAYQIFRNVAKPGSKYIDE+D
Sbjct: 421 INVIRGSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEED 480

Query: 526 LFRFMSKEEVDNMLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELN 585
           LFRFMSKEE+DN+LPLFEG  ETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELN
Sbjct: 481 LFRFMSKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELN 540

Query: 586 KLASATVAIVIIVEWLLMMGLLTTQVLVFFSSQLLLVVFMFGNTARTVFEGIIFVFVMHP 645
           KLASA V IVII+EWLL+MG LTTQVLVF SSQ+LLVVFMFGNTARTVFE IIFVFVMHP
Sbjct: 541 KLASAVVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHP 600

Query: 646 FDVGDRCVVDGVQLIVEEMNILTTIFLRCDNEKIFYPNSVLATKPISNFYRSPEMGDSIE 705
           FDVGDRCVVDGVQ++VEEMNILTTIFLR DNEKIFYPNSVLATKPISN+YRSPEM DSI+
Sbjct: 601 FDVGDRCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSID 660

Query: 706 FSVDFSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLHVNHTINFQ 765
           FSVDFSTSIESIGALKARIK+Y+ESKPQFWRPN+S++VKEIENVNKMK+ L VNHTINFQ
Sbjct: 661 FSVDFSTSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQ 720

Query: 766 NYGDKSSRRSELVLELKKAFEDLGIKYHLLAQEVQLNYVSSAAPMVP--PSQR 812
           NYGDKS+RRS+LVLELKK FEDLGIKYHLL Q VQLNY SSAA  VP  PSQR
Sbjct: 721 NYGDKSNRRSDLVLELKKIFEDLGIKYHLLPQAVQLNYASSAAGGVPLPPSQR 768

BLAST of Cp4.1LG04g10920.1 vs. TAIR 10
Match: AT5G12080.1 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 672.9 bits (1735), Expect = 3.2e-193
Identity = 372/718 (51.81%), Postives = 504/718 (70.19%), Query Frame = 0

Query: 90  RSFRCTIPKSVVG----SSSSHEIARM--NPYKPPKIPAESAV---RRPSFARSSFSKPK 149
           RS     P+S  G     S S EI+++  +P KPP+ P ++ V   +R SFARS +SKPK
Sbjct: 28  RSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPK 87

Query: 150 SRLVEPPCPHGGSLAEETFAEKSISGSPFCSSTKMDSPARITAVTSPREALKSVPTTPRT 209
           SR V+P CP   S+ EE   E+  +G  F  ++  +   R  +V SP       P TP  
Sbjct: 88  SRFVDPSCPVDTSILEEEVREQLGAGFSFSRASPNNKSNR--SVGSP------APVTPSK 147

Query: 210 PFTGNEEEDDEEVYRTAELKVKERSGKKLTKTILIEWFSFLCLTGCLISTLTVDKLATKE 269
                E+++DEE+Y+  +L  + RS  K++   LIE   F+ +   L+++LT++ L    
Sbjct: 148 VVV--EKDEDEEIYKKVKLNREMRS--KISTLALIESAFFVVILSALVASLTINVLKHHT 207

Query: 270 IWELRLWKWCVLVLVIFSGRLFSRWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIW 329
            W L +WKWCVLV+VIFSG L + WF+  +VFLIE NFLL+RKVLYFV+GL+KSV VFIW
Sbjct: 208 FWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVLYFVHGLKKSVQVFIW 267

Query: 330 LTLVLLAWGLLFDRTSKRSKKGHAILNYVTRALGGSLIGAAIWLVKTLLVKILAASFQCT 389
           L L+L+AW LLF+   KRS     +L  +TR L   L GA  WLVKTLL+KILAA+F   
Sbjct: 268 LCLILVAWILLFNHDVKRSPAATKVLKCITRTLISILTGAFFWLVKTLLLKILAANFNVN 327

Query: 390 RFFDRIQESIFHQYILRTLSGPPLMEMAKGIGRTAGTGQLSFRHLKKVRDGGKERKEEVI 449
            FFDRIQ+S+FHQY+L+TLSG PLME A+ +GR   TG LSF  + K    G  ++++VI
Sbjct: 328 NFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPSTGHLSFATVVK---KGTVKEKKVI 387

Query: 450 DVDKLKKMNQEKISAWTMRGLIHVIRSSGLSTISNTI-ENFRDEELEQKDKEIHSEWEAR 509
           D+ K+ KM +EK+SAWTMR L+  +R+SGLSTIS+T+ E    E  EQ D+EI SE EA 
Sbjct: 388 DMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEGKEQADREITSEMEAL 447

Query: 510 AAAYQIFRNVAKPGSKYIDEDDLFRFMSKEEVDNMLPLFEGAVETGKIKRKTLKNWLVNV 569
           AAAY +FRNVA+P   YI+E+DL RFM KEEVD + PLF+GA ETG+I RK    W+V V
Sbjct: 448 AAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAETGRITRKAFTEWVVKV 507

Query: 570 YVERKSLAHSLNDTKTAIEELNKLASATVAIVIIVEWLLMMGLLTTQVLVFFSSQLLLVV 629
           Y  R++LAHSLNDTKTA+++LNKL +A + +V +V WLL++ + TT+VL+FFS+QL+ + 
Sbjct: 508 YTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVATTKVLLFFSTQLVALA 567

Query: 630 FMFGNTARTVFEGIIFVFVMHPFDVGDRCVVDGVQLIVEEMNILTTIFLRCDNEKIFYPN 689
           F+ G+T + +FE I+FVFVMHP+DVGDRCVVDGV ++VEEMN+LTT+FL+ +NEK++YPN
Sbjct: 568 FIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPN 627

Query: 690 SVLATKPISNFYRSPEMGDSIEFSVDFSTSIESIGALKARIKSYIESKPQFWRPNHSILV 749
           +VLATKPISN++RSP MG+++EFS+ FST +  I  LK RI  Y+E  PQ W P HS++V
Sbjct: 628 AVLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSVVV 687

Query: 750 KEIENVNKMKMGLHVNHTINFQNYGDKSSRRSELVLELKKAFEDLGIKYHLLAQEVQL 798
           KEIEN+NK+KM L+ +HTI FQ   +++ RR+EL L +K+  EDL I Y LL Q++ L
Sbjct: 688 KEIENMNKLKMALYSDHTITFQENRERNLRRTELSLAIKRMLEDLHIDYTLLPQDINL 730

BLAST of Cp4.1LG04g10920.1 vs. TAIR 10
Match: AT5G12080.2 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 672.9 bits (1735), Expect = 3.2e-193
Identity = 372/718 (51.81%), Postives = 504/718 (70.19%), Query Frame = 0

Query: 90  RSFRCTIPKSVVG----SSSSHEIARM--NPYKPPKIPAESAV---RRPSFARSSFSKPK 149
           RS     P+S  G     S S EI+++  +P KPP+ P ++ V   +R SFARS +SKPK
Sbjct: 28  RSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPK 87

Query: 150 SRLVEPPCPHGGSLAEETFAEKSISGSPFCSSTKMDSPARITAVTSPREALKSVPTTPRT 209
           SR V+P CP   S+ EE   E+  +G  F  ++  +   R  +V SP       P TP  
Sbjct: 88  SRFVDPSCPVDTSILEEEVREQLGAGFSFSRASPNNKSNR--SVGSP------APVTPSK 147

Query: 210 PFTGNEEEDDEEVYRTAELKVKERSGKKLTKTILIEWFSFLCLTGCLISTLTVDKLATKE 269
                E+++DEE+Y+  +L  + RS  K++   LIE   F+ +   L+++LT++ L    
Sbjct: 148 VVV--EKDEDEEIYKKVKLNREMRS--KISTLALIESAFFVVILSALVASLTINVLKHHT 207

Query: 270 IWELRLWKWCVLVLVIFSGRLFSRWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIW 329
            W L +WKWCVLV+VIFSG L + WF+  +VFLIE NFLL+RKVLYFV+GL+KSV VFIW
Sbjct: 208 FWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVLYFVHGLKKSVQVFIW 267

Query: 330 LTLVLLAWGLLFDRTSKRSKKGHAILNYVTRALGGSLIGAAIWLVKTLLVKILAASFQCT 389
           L L+L+AW LLF+   KRS     +L  +TR L   L GA  WLVKTLL+KILAA+F   
Sbjct: 268 LCLILVAWILLFNHDVKRSPAATKVLKCITRTLISILTGAFFWLVKTLLLKILAANFNVN 327

Query: 390 RFFDRIQESIFHQYILRTLSGPPLMEMAKGIGRTAGTGQLSFRHLKKVRDGGKERKEEVI 449
            FFDRIQ+S+FHQY+L+TLSG PLME A+ +GR   TG LSF  + K    G  ++++VI
Sbjct: 328 NFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPSTGHLSFATVVK---KGTVKEKKVI 387

Query: 450 DVDKLKKMNQEKISAWTMRGLIHVIRSSGLSTISNTI-ENFRDEELEQKDKEIHSEWEAR 509
           D+ K+ KM +EK+SAWTMR L+  +R+SGLSTIS+T+ E    E  EQ D+EI SE EA 
Sbjct: 388 DMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEGKEQADREITSEMEAL 447

Query: 510 AAAYQIFRNVAKPGSKYIDEDDLFRFMSKEEVDNMLPLFEGAVETGKIKRKTLKNWLVNV 569
           AAAY +FRNVA+P   YI+E+DL RFM KEEVD + PLF+GA ETG+I RK    W+V V
Sbjct: 448 AAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAETGRITRKAFTEWVVKV 507

Query: 570 YVERKSLAHSLNDTKTAIEELNKLASATVAIVIIVEWLLMMGLLTTQVLVFFSSQLLLVV 629
           Y  R++LAHSLNDTKTA+++LNKL +A + +V +V WLL++ + TT+VL+FFS+QL+ + 
Sbjct: 508 YTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVATTKVLLFFSTQLVALA 567

Query: 630 FMFGNTARTVFEGIIFVFVMHPFDVGDRCVVDGVQLIVEEMNILTTIFLRCDNEKIFYPN 689
           F+ G+T + +FE I+FVFVMHP+DVGDRCVVDGV ++VEEMN+LTT+FL+ +NEK++YPN
Sbjct: 568 FIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPN 627

Query: 690 SVLATKPISNFYRSPEMGDSIEFSVDFSTSIESIGALKARIKSYIESKPQFWRPNHSILV 749
           +VLATKPISN++RSP MG+++EFS+ FST +  I  LK RI  Y+E  PQ W P HS++V
Sbjct: 628 AVLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSVVV 687

Query: 750 KEIENVNKMKMGLHVNHTINFQNYGDKSSRRSELVLELKKAFEDLGIKYHLLAQEVQL 798
           KEIEN+NK+KM L+ +HTI FQ   +++ RR+EL L +K+  EDL I Y LL Q++ L
Sbjct: 688 KEIENMNKLKMALYSDHTITFQENRERNLRRTELSLAIKRMLEDLHIDYTLLPQDINL 730

BLAST of Cp4.1LG04g10920.1 vs. TAIR 10
Match: AT5G12080.3 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 672.9 bits (1735), Expect = 3.2e-193
Identity = 372/718 (51.81%), Postives = 504/718 (70.19%), Query Frame = 0

Query: 90  RSFRCTIPKSVVG----SSSSHEIARM--NPYKPPKIPAESAV---RRPSFARSSFSKPK 149
           RS     P+S  G     S S EI+++  +P KPP+ P ++ V   +R SFARS +SKPK
Sbjct: 28  RSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPK 87

Query: 150 SRLVEPPCPHGGSLAEETFAEKSISGSPFCSSTKMDSPARITAVTSPREALKSVPTTPRT 209
           SR V+P CP   S+ EE   E+  +G  F  ++  +   R  +V SP       P TP  
Sbjct: 88  SRFVDPSCPVDTSILEEEVREQLGAGFSFSRASPNNKSNR--SVGSP------APVTPSK 147

Query: 210 PFTGNEEEDDEEVYRTAELKVKERSGKKLTKTILIEWFSFLCLTGCLISTLTVDKLATKE 269
                E+++DEE+Y+  +L  + RS  K++   LIE   F+ +   L+++LT++ L    
Sbjct: 148 VVV--EKDEDEEIYKKVKLNREMRS--KISTLALIESAFFVVILSALVASLTINVLKHHT 207

Query: 270 IWELRLWKWCVLVLVIFSGRLFSRWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIW 329
            W L +WKWCVLV+VIFSG L + WF+  +VFLIE NFLL+RKVLYFV+GL+KSV VFIW
Sbjct: 208 FWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVLYFVHGLKKSVQVFIW 267

Query: 330 LTLVLLAWGLLFDRTSKRSKKGHAILNYVTRALGGSLIGAAIWLVKTLLVKILAASFQCT 389
           L L+L+AW LLF+   KRS     +L  +TR L   L GA  WLVKTLL+KILAA+F   
Sbjct: 268 LCLILVAWILLFNHDVKRSPAATKVLKCITRTLISILTGAFFWLVKTLLLKILAANFNVN 327

Query: 390 RFFDRIQESIFHQYILRTLSGPPLMEMAKGIGRTAGTGQLSFRHLKKVRDGGKERKEEVI 449
            FFDRIQ+S+FHQY+L+TLSG PLME A+ +GR   TG LSF  + K    G  ++++VI
Sbjct: 328 NFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPSTGHLSFATVVK---KGTVKEKKVI 387

Query: 450 DVDKLKKMNQEKISAWTMRGLIHVIRSSGLSTISNTI-ENFRDEELEQKDKEIHSEWEAR 509
           D+ K+ KM +EK+SAWTMR L+  +R+SGLSTIS+T+ E    E  EQ D+EI SE EA 
Sbjct: 388 DMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEGKEQADREITSEMEAL 447

Query: 510 AAAYQIFRNVAKPGSKYIDEDDLFRFMSKEEVDNMLPLFEGAVETGKIKRKTLKNWLVNV 569
           AAAY +FRNVA+P   YI+E+DL RFM KEEVD + PLF+GA ETG+I RK    W+V V
Sbjct: 448 AAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAETGRITRKAFTEWVVKV 507

Query: 570 YVERKSLAHSLNDTKTAIEELNKLASATVAIVIIVEWLLMMGLLTTQVLVFFSSQLLLVV 629
           Y  R++LAHSLNDTKTA+++LNKL +A + +V +V WLL++ + TT+VL+FFS+QL+ + 
Sbjct: 508 YTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVATTKVLLFFSTQLVALA 567

Query: 630 FMFGNTARTVFEGIIFVFVMHPFDVGDRCVVDGVQLIVEEMNILTTIFLRCDNEKIFYPN 689
           F+ G+T + +FE I+FVFVMHP+DVGDRCVVDGV ++VEEMN+LTT+FL+ +NEK++YPN
Sbjct: 568 FIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPN 627

Query: 690 SVLATKPISNFYRSPEMGDSIEFSVDFSTSIESIGALKARIKSYIESKPQFWRPNHSILV 749
           +VLATKPISN++RSP MG+++EFS+ FST +  I  LK RI  Y+E  PQ W P HS++V
Sbjct: 628 AVLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSVVV 687

Query: 750 KEIENVNKMKMGLHVNHTINFQNYGDKSSRRSELVLELKKAFEDLGIKYHLLAQEVQL 798
           KEIEN+NK+KM L+ +HTI FQ   +++ RR+EL L +K+  EDL I Y LL Q++ L
Sbjct: 688 KEIENMNKLKMALYSDHTITFQENRERNLRRTELSLAIKRMLEDLHIDYTLLPQDINL 730

BLAST of Cp4.1LG04g10920.1 vs. TAIR 10
Match: AT5G19520.1 (mechanosensitive channel of small conductance-like 9 )

HSP 1 Score: 618.2 bits (1593), Expect = 9.2e-177
Identity = 334/688 (48.55%), Postives = 472/688 (68.60%), Query Frame = 0

Query: 115 YKPPKIPA-ESAVRRPSFARSSFSKPKSRLVEPPCPHGGSLAEE----TFAEKSISGSPF 174
           +KPPKIP+ E  VRR S +RS +SKPKSR  E       S  EE    +  E+  +GS  
Sbjct: 67  HKPPKIPSPEGLVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENGGRSLREQFGAGSFA 126

Query: 175 CSSTKMDSPARITAVTSPREALKSVPTTPRTPFTGNEEEDDEEVYRTAELKVKERSGKKL 234
             S    SP          ++ +SV +   +     E +++EE+Y+  +L   +RSG K 
Sbjct: 127 RGSFDRASP--------NNKSNRSVASAALSKVAEEEPDENEEIYKKVKLHRVKRSGMK- 186

Query: 235 TKTILIEWFSFLCLTGCLISTLTVDKLATKEIWELRLWKWCVLVLVIFSGRLFSRWFINC 294
                +E   F+ + G LI +LT+D +    IW L  WKWCVLV+V  SG L + WF++ 
Sbjct: 187 -PLAFLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLSGMLVTNWFMHF 246

Query: 295 LVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLTLVLLAWGLLFDRTSKRSKKGHAILNYV 354
           +VF+IE+N+LL++KVLYFV+GL+K+V VFIW +LVL+AW  LFD   KR++K    L+++
Sbjct: 247 VVFIIEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGDVKRTRKTKRFLDFI 306

Query: 355 TRALGGSLIGAAIWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLMEMAK 414
           T  +   L+G+ ++LVKT  +K+LA+ F    FF+RIQES+FHQY+L+TLSGPPL+E A+
Sbjct: 307 TWTIVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQTLSGPPLIEEAE 366

Query: 415 GIGRTAGTGQLSFRHLKKVRDGGKERKEEVIDVDKLKKMNQEKISAWTMRGLIHVIRSSG 474
            +GR   TG LSF   K     GK + ++VID+ K+ +M QEK+SAWTMR LI  + +SG
Sbjct: 367 NVGRVPSTGHLSFTRTK----DGKVKDKKVIDMGKVHRMKQEKVSAWTMRVLIEAVGTSG 426

Query: 475 LSTISNTIENFRDEELEQKDKEIHSEWEARAAAYQIFRNVAKPGSKYIDEDDLFRFMSKE 534
           +STIS+T++   +++ E+ DKEI +E EA AAAY +F NVAKP   YI+EDDL RFM KE
Sbjct: 427 ISTISSTLDEVNNKK-ERTDKEITNEMEAVAAAYDVFNNVAKPNHNYIEEDDLLRFMIKE 486

Query: 535 EVDNMLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASATVA 594
           EVD +LPL E A +TGKI RKT   W+VNVY  RK++ HSLNDTKTA+++L+KL +  + 
Sbjct: 487 EVDLVLPLIEDA-DTGKITRKTFTEWVVNVYTSRKTIGHSLNDTKTAVKQLDKLITGILT 546

Query: 595 IVIIVEWLLMMGLLTTQVLVFFSSQLLLVVFMFGNTARTVFEGIIFVFVMHPFDVGDRCV 654
           ++  + W++++ + +T++L+ FSSQ L + FM G+T + +FE  +FVFVMHP+DVGDRCV
Sbjct: 547 VITFIVWMVLLDIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFVMHPYDVGDRCV 606

Query: 655 VDGVQLIVEEMNILTTIFLRCDNEKIFYPNSVLATKPISNFYRSPEMGDSIEFSVDFSTS 714
           VDGV L+VEE+++LTT+FL+ DNEK+FYPNSVL +KPISNFYRSP+MGD ++F + FST 
Sbjct: 607 VDGVMLLVEEIDLLTTVFLKIDNEKVFYPNSVLISKPISNFYRSPDMGDYVDFGIAFSTP 666

Query: 715 IESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLHVNHTINFQNYGDKSSR 774
            E IG LK +I  Y+ +  Q W P   ++V+ IEN+NK+ + + V HTINFQ Y +KS R
Sbjct: 667 AEKIGCLKGKIGEYLVANSQHWYPEAQVMVRAIENMNKLVLNILVQHTINFQVYVEKSLR 726

Query: 775 RSELVLELKKAFEDLGIKYHLLAQEVQL 798
           R+ L++ +K+  EDL I Y LL Q+V L
Sbjct: 727 RTALIIAIKRILEDLEIDYTLLPQDVNL 738

BLAST of Cp4.1LG04g10920.1 vs. TAIR 10
Match: AT3G14810.1 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 516.9 bits (1330), Expect = 2.9e-146
Identity = 304/716 (42.46%), Postives = 444/716 (62.01%), Query Frame = 0

Query: 127 RRPSFARSSFSKPKSRLVEPPCPHGGSLAEETFAEKSISGSPFCSSTKMDSPARITAVTS 186
           ++P  +R   +K KSRL +PP P                  P    T+M S  R     S
Sbjct: 185 KKPMLSR---NKTKSRLQDPPTP----------------THPAIDKTEMKSGRRSGIFKS 244

Query: 187 PREALKSVPTTPRTPFTGNEEEDDEEVYRTAELKVKERSGKKLTKTILIEWFSFLCLTGC 246
               L   P        G EEE++E+ +   +L  +E    KL+  + +EW S + +   
Sbjct: 245 --GFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLP-EEFKRDKLSFWVFLEWISLVLIVTS 304

Query: 247 LISTLTVDKLATKEIWELRLWKWCVLVLVIFSGRLFSRWFINCLVFLIERNFLLKRKVLY 306
           L+ +LT+  L  K  W+L LWKW V VLV+  GRL S W +  +VFL+E+NF  +++VLY
Sbjct: 305 LVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRKRVLY 364

Query: 307 FVYGLRKSVIVFIWLTLVLLAWGLLFDRTSKRSKKGHAILNYVTRALGGSLIGAAIWLVK 366
           FVYG+RKSV   +WL LVLLAW  LFD+  +R  +  A L YVTR L   L+   IWLVK
Sbjct: 365 FVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTA-LRYVTRVLVCLLVALIIWLVK 424

Query: 367 TLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLMEM-------------AKGIGR 426
           T+LVK+LA+SF  + +FDRIQES+F QY++ TLSGPPLME+              K + +
Sbjct: 425 TILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQRMEEEEQQVAEDVKSLEK 484

Query: 427 TAGTG-----QLSFRHLKKV-------RDGGKERKE-EVIDVDKLKKMNQEKISAWTMRG 486
            AG       + + +   KV       R G K  ++ E I +D+LK+MN + +SAW M+ 
Sbjct: 485 LAGAKLPPALKATVKSFMKVGKSPGLNRIGSKRGEDGEGIRIDQLKRMNTKNVSAWNMKR 544

Query: 487 LIHVIRSSGLSTISNTIENFRDEELEQKD-KEIHSEWEARAAAYQIFRNVAKPGSKYIDE 546
           L+++I    +ST+    +N +D   E +D   I SE+EA+ AA +IF NV +PGS+YI  
Sbjct: 545 LMNIILKGAISTLD---QNMQDTTQEDEDATHIRSEYEAKCAARKIFHNVTEPGSRYIYL 604

Query: 547 DDLFRFMSKEEVDNMLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEE 606
           +D  RF+ +EE +  + LFEGA E+ KI +  LKNW+V  + ER++LA +LNDTKTA++ 
Sbjct: 605 EDFLRFLCEEEAERAMALFEGASESDKISKSCLKNWVVKAFRERRALALTLNDTKTAVDR 664

Query: 607 LNKLASATVAIVIIVEWLLMMGLLTTQVLVFFSSQLLLVVFMFGNTARTVFEGIIFVFVM 666
           L+++ +  + I+II+ WLL++G+ TT+ L+  SSQLLLV F+FGN+ +T+FE IIF+FVM
Sbjct: 665 LHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVM 724

Query: 667 HPFDVGDRCVVDGVQLIVEEMNILTTIFLRCDNEKIFYPNSVLATKPISNFYRSPEMGDS 726
           HPFDVGDRC +DGVQL+VEEMNILTT+FLR DN+KI YPNSVL TKPI+N+YRSP+MGD+
Sbjct: 725 HPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDA 784

Query: 727 IEFSVDFSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLHVNHTIN 786
           +EF V  +T  E I A+K RI SY+++K  +W P   I+   ++++N +K+ + + H +N
Sbjct: 785 VEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMN 844

Query: 787 FQNYGDKSSRRSELVLELKKAFEDLGIKYHLLAQEVQLNYV-----SSAAPMVPPS 811
            Q+ G++  RR  L+ E+ K   +L I+Y L    + +  +      +++  +PPS
Sbjct: 845 HQDMGERYIRRGLLLEEVGKTCRELDIEYRLYPLNINVRSLPPTANPTSSDRIPPS 874